| letter: highly purified insulins. | | 1976 | 58350 |
| [alpha fetoprotein--its physiology and clinical significance]. | | 1976 | 58402 |
| ia antigens and fc receptors. | the function of fc receptors (fcr) on mouse spleen cells as detected by a sensitive rosetting system using antibody-labeled bovine erythrocytes as indicator cells was shown to be inhibited by anti-ia antiserum. furthermore, the ia specificities of each i-region sublocus (i-1a, i-1b, i-c) seem to be associated with frr, since fcr function was also inhibited when the blocking anti-ia antiserum recognized only restricted ia specificities. antiserum directed against antigens coded for by the k-end o ... | 1976 | 58439 |
| [effect of a gestagen used for the induction of synchronous estrus and fertilization of cattle]. | the preparations synchrosyn, gestafortin, and bovisynchron, having chlormadinonacetate as a common active ingredient, were used to induce synchronous estrus and fertilization in heifers. a total of 254 animals were involved belonging to the following breeds: bulgarian simmental, bulgarian red, bulgarian brown, and black pied. all three preparations were administered orally: synchrosyn and gestafortin were given with the forage at the rate of 10 mg chlormadinonacetate, and bovisynchron was offere ... | 1975 | 58466 |
| [level of viral antibodies in normal and specific swine and bovine gamma globulin preparations]. | studied were 15 series of normal bovine gamma-globulin, 5 series of normal swine gamma-globulin, 7 series of aujeszky gamma-globulin, 6 series of specific gamma-globulin against edema disease of pigs, 4 series of specific gamma-globulin against paratyphus, 5 series of mixoglobulin-70, and one series of swine poly-globulin. all series of gamma-globulin preparations were studied for the presence of antibodies against mixovirus parainfluenza-3 through the hemagglutinationinhibition reaction, antibo ... | 1975 | 58467 |
| hereditary zinc deficiency (adema disease) in cattle, an animal parallel to acrodermatitis enteropathica. | adema disease and acrodermatitis enteropathica, two parallel syndromes in calves of friesian descent and in man, are described. both are congenital zinc deficiency disorders with a lethal course if left untreated. complete recovery follows oral zinc therapy. symptoms and findings are set out in table i. diseased calves may serve as animal models for further studies on acrodermatitis enteropathica and the biological role of zinc. | 1976 | 58525 |
| characterization of fluorescein isothiocyanate. ii. absorption and fluorescence after conjugation to human- and rabbit-gamma-globulin and bovine serum albumin. | the alteration of extinctions of both fitc isomers by coupling to bovine serum albumin, human, and rabbit-gamma-globulin were studied. the extinction coefficients of these ftc conjugates were established. the emission characteristics of the fitc isomers and their conjugates were investigated under conditions normaly used in fluorescence microscopy. fitc i was found as the most suitable marker for human- and rabbit-gamma-globulin. in contrast the fluorescence of ftc ii-bsa conjugates is stronger ... | 1976 | 58532 |
| indomethacin and the corneal immune response. | to study the effect of treating rabbits with a locally administered noncorticosteroid anti-inflammatory drug, we gave 26 rabbits unilateral subconjunctival injections of 15 mg of indomethacin suspension daily for 16 days, starting one day before the intracorneal injection of the same eye with bovine gamma globulin. we graded the eyes clinically and killed groups of rabbits on postimmunization days 6, 9, 12, and 15. we determined the number of antibody-forming cells in the homolateral cervical ly ... | 1976 | 58559 |
| changes in serum concentration of phagocytosis-stimulating factor in experimentally induced bovine anaplasmosis: preliminary findings. | to determine whether correlation exists between serum concentration of phagocytosis-stimulating factor (psf) and in vivo phagocytic activity, 2 splenectomized steers were inoculated with anaplasma marginale, and their serum psf concentrations were monitored. at the time of the anemic crisis, serum psf concentrations were elevated five to tenfold. | 1976 | 58572 |
| induction of tolerance in adult guinea-pigs by two antigen dosage levels and by antigenic competition. | the effect of prior injection of increasing doses of dinitrophenylated bovine serum albumin (dnp-bsa) mixed in freund's complete (fca) or incomplete (ifa) adjuvants on the antibody response to a subsequent challenge with an optimal immunizing dose of dnp-bsa in fca has been examined and compared with the effect of competition with the unrelated antigen bovine gamma-globulin (bgg). an unresponsive state was induced by pretreatments with either a minimal dose of antigen, a large antigen excess or ... | 1975 | 58574 |
| [presence of antigen sv40/t in the nuclear and cytoplasmic fraction of sv40- and para-transformed cells]. | | 1975 | 58585 |
| [application of microcalorimetry to enzyme kinetic studies]. | | 1975 | 58589 |
| [lipid composition of bovine thyroid. v. lipophilic component of neutral glycolipids]. | | 1975 | 58596 |
| [estrus synchronization in cattle by means of chlormadinone acetate ("bovisynchron" jenapharm) in the tropics. ii. first results of the estrus synchronization of cattle with "bovisynchron" at the republic of mali]. | the first experiments are reported on oestrus synchronization in cattle in the republic of mali. the animals recieved bovisynchron twice a day orally over a period of 15 days. the synchronization effect was marked; insemination was performed on the 5th and 6th day p.s. the intensity of the oestrus and especially the results of pregnancy are in close relationship to the breeding conditions and the general status of the animals. the oestrus synchronization is considered a favourable biotechnical m ... | 1975 | 58658 |
| [immunofluorescent study of antibodies to streptococcus group a polysaccharides in connective tissue sections]. | purified antibodies against a specific determinant of group a streptococcal polysaccharide were obtained. these antibodies were investigated by the immunofluorescent method on tissue sections of the heart and heart valves. no cross-reactions between group a streptococcal polysaccharide and mammalian connective tissue were revealed. | 1976 | 58683 |
| inhibition of mitochondrial electron transport systems by phosvel and some environmental conversion products. | | 1976 | 58687 |
| characterization of streptococcus bovis bacteriophages. | about 25 streptococcus bovis bacteriophages were isolated from abattoir wastes, bovine rumen fluid, and lysogenic strains of s. bovis. eight phages were selected and characterized by morphology, stability, rate of adsorption, single-step growth curve, serum neutralization, and antigenic relationship. two distinct morphological phage types were found, one of which has not been previously reported for group d streptococci. | 1976 | 58706 |
| factors controlling igm and igg antibody responses to denatured trypanosome dna. | rabbits immunized with complexes of denatured calf thymus dna and methylated bovine serum albumin (mbsa) produced only igm antibodies to denatured dna, confirming the results of previous studies. in contrast, both igm and igg antibodies were induced by mbsa complexes of trypanosoma brucei dna. a similar response was observed by gruenewald and stollar (1973) to t2 bacteriophage dna, in which the normal base cytosine is replaced by hydroxymethylcytosine. t2 bacteriophage dna is known to have a hig ... | 1976 | 58830 |
| bovine leptospirosis. | | 1976 | 58876 |
| the effects of glutaraldehyde treatment and horesradish peroxidase conjugation on the immunoreactivity of bovine myelin encephalitogenic protein. | the effects of the immunoreactivity of bovine myelin encephalitogenic protein (ep) of treatment with glutaraldehyde and conjugation with horeradish peroxidase (hrp) were investigated by complement fixation and immunohistochemistry. both glutaraldehyde treatment and hrp conjugation of ep decreased but did not abolish the reactivity of ep with rabbit anti-ep. conjugation of ep to hrp by the two-step method of avrameas had less detrimental effects on the immunochemical reactivity of ep than did the ... | 1976 | 58926 |
| n-acetylneuraminic acid molecules as possible serotonin binding sites. | | 1976 | 58968 |
| non-protein constituents in myelin basic protein preparations. | | 1976 | 58970 |
| effect of phthalate plasticizer on blood compatibility of polyvinyl chloride. | the amount of a phthalate plasticizer on the surface of a sheet of polyvinyl chloride used in the fabrication of blood storage bags was quantified using attenuated total reflectance spectroscopy, water contact angle measurement, and weight loss due to methanol extraction. water wettability increased as the amount of surface phthalate extracted by methanol increased, which indicates that the accumulation of phthalate on the surface increases hydrophobicity. the extraction of phthalate by methanol ... | 1976 | 58987 |
| is lactase the receptor and uncoating enzyme for infantile enteritis (rota) viruses? | rotaviruses are now regarded as important causes of diarrhoea in man, cattle, pigs, mice, and possibly other animals. characteristically, disease occurs in newborn and young animals, and infection seems limited to the differentiated gut epithelial cells. the major surface polypeptide of the calf scours rotavirus is glycosylated, and highly purified beta-galactosidase (lactase) interacts with the virus in vitro causing removal of the outer shell of the capsid (uncoating). it is suggested that lac ... | 1976 | 59021 |
| chemical mediators of anaphylaxis (histamine, 5-ht, and srs-a) released from horse lung and leukocytes in vitro. | horses were sensitized to bovine plasma in freund's complete adjuvant. leukocytes, separated from venous blood, yielded histamine upon incubation with bovine plasma. ioslated lung fragments incubated with bovine plasma liberated histamine and 5-ht, but not srs-a. pulmonary veins obtained from the same animals contracted to histamine, 5-ht and to antigen (schultz-dale reaction). histamine and 5-ht probably contribute to immediate-type hypersensitivity in horses whereas the role of srs-a is not pr ... | 1976 | 59377 |
| protection of cattle against experimental haemorrhagic septicaemia by the capsular antigens of pasteurella multocida, types b and e. | aluminum hydroxide adjuvant vaccines containing endotoxin-free capsular antigens of pasteurella multocida, types b and e, were administered to cattle. dose dependent serological responses were observed which were similar for both antigens. the immunised cattle were subjected to intravenous challenge by a virulent type e strain. all animals which received the highest vaccine dose survived and all unimmunised control animals died and a vaccine dose-response relationship was obtained. the results o ... | 1976 | 59379 |
| platelet retention in glass bead columns: its mechanism and the effect of plasma proteins. | | 1976 | 59397 |
| a combined silver and acetylcholinesterase method for staining intramuscular innervation. | a combined acetylcholinesterase and silver stain for demonstrating the intramuscular innervation of fresh frozen tissue is described. intramuscular nerves, subterminal axons, and motor end plates are simultaneously stained brown or black with minimal staining of connective tissue and muscle fibers in longitudinal sections 30-100 mu thick. the method has been applied to fetal and adult rat, porcine, and bovine skeletal muscle. antemortem and postmortem tissue samples stained equally well. the met ... | 1976 | 59425 |
| specificity of antigenic fractions of tuberculin. | purified protein derivative from mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 v/40 ma in ph 4.2 acetic acid-pyridine buffer. further purification of one of the fractions on sephadex g 25 column and by acid hydrolysis yielded antigen "ps" eliciting tuberculin reaction only in animals vaccinated with m. bovis bcg but not in those sensitized with m. avium and some fast-growing mycobacteria. the specificity of antigen "ps" was confirmed in vitro: the antigen induced ... | 1976 | 59518 |
| non-specific antimicrobial cellular resistance in sensitized guinea pigs. | specific delayed-type hypersensitivity was induced in guinea pigs with bovine albumin + complete freund adjuvant, bovine gamma globulin + complete freund adjuvant and bcg vaccine. the animals were subsequently tested for nonspecific antimicrobial resistance. sensitized and control groups were challenged intraperitoneally with listeria monocytogenes 2 hr after reinjection with the sensitizing antigen. the listeria content of the spleens was determined 1 or 5 days after the infection. the number o ... | 1976 | 59519 |
| antigenic differences among bovine and porcine factor viii concentrates. | three anti-factor-viii antibodies from hemophiliacs were reacted with samples of batches of maws commerical bovine and porcine factor viii concentrates manufactured over a 12-year period. the apparent antibody concentrations varied widely with the different batches of concentrates. the variations are probably due to intrinsic differences in the antigenic nature of the factor viii in the different preparations. with the older porcine concentrates, the low apparent concentrations may be in part du ... | 1976 | 59540 |
| myelin basic protein: a substance that releases immunoreactive growth hormone in vitro. | | 1976 | 59596 |
| inhibition of denaturation of human gamma globulin by a mixture of l-histidine, l-cystine, and copper, and its clinical implication in rheumatoid arthritis. | a mixture of histidine, cystine, and copper mimicked gold thiomalate, n-ethylmaleimide, and p-chloro-mercuribenzoic acid in inhibiting sulfhydryl-disulfide interchange-mediated denaturation of human gamma globulin, bovine serum albumin, and diluted human serum. measurable inhibitory effects were obtained with a mixture of physiologic concentrations of l-histidine, l-cystine, and copper. this work suggests a mechanism by which the hypohistidinemia of rheumatoid arthritis could contribute to the p ... | 1976 | 59600 |
| conformational transitions of non-helical proteins effected by dodecyl sulfate. circular dichroism of alpha1-acid glycoprotein, bence jones protein, carbonic anhydrase b, deoxyribonuclease, pepsinogen, and plasminogen. | circular dichroism (cd) of serum alpha1-acid glycoprotein, urinary bence jones protein, human carbonic anhydrase b, deoxyribonuclease from bovine pancreas, porcine pepsinogen, and plasminogen from human serum was tested in the absence and presence of 0.005-0.05 m sodium dodecyl sulfate. it was found that in all cases the cd spectra of these proteins were modified by the dodecyl sulfate into spectra indicating the presence of a moderate content of alpha-helix. the transitions were enhanced by add ... | 1976 | 59606 |
| a kinetic study of the inhibition of human and bovine trypsins and chymotrypsins by the inter-alpha-inhibitor from human plasma. | human plasma inter-alpha-inhibitor forms 1:1 inactive complexes with human and bovine trypsins (ec 3.4.21.4) and chymotrypsins (ec 3.4.21.1). the association and dissociation rate constants as well as the equilibrium dissociation constants (ki) of the complexes formed of inter-alpha-inhibitor and the four proteases have been measured. the most stable complexes are those formed with the bovine enzymes. for instance, ki = 2.1-10-11 m for bovine trypsin whereas ki = 1.2 - 10-8 m for human trypsin. ... | 1976 | 59607 |
| binding of 3,5-diiodotyrosine to serum proteins. | the reversible binding of 3,5-diiodotyrosine (dit) to human and bovine serum protein and to purified human serum prealbumin and human and bovine albumin has been studied by equilibrium dialysis. maximum binding occurred at ph 8.6-9.0. human serum bound dit less than did bovine serum. adult ox and fetal calf sera showed similar binding. the main dit-binding protein of human serum was prealbumin. it showed a single affinity site with a ka of 0.85 x 10(6) m-1 at ph 8.6 and 0.40 x 10(6) m-1 at ph 7. ... | 1976 | 59643 |
| aspects of the secondary antibody response to ox insulin in the hartley guinea-pig; the use of chemically modified ox insulin to delineate the antigenic determinants of ox insulin. | the use of a quinea-pig model to study the immunogenicity of the insulin molecule is presented. the hartley guinea-pig has been shown consistently to form antibody to ox insulin, when given in a water-in-oil emulsion containing pertussis vaccine as adjuvant. after log transformation of standardized antibody titres to iodo-ox insulin, a valid statistical comparison of the antibody response to different ox insulin preparations could be made. antibody cross-reacting with ox insulin, but not iodo-ox ... | 1976 | 59648 |
| immunological investigations of antigenicity and specificity of soluble collagen fractions. iv. anaphylaxis and allergy experiments. | collagen prepared from rat, calf, or pig skin was tested on guinea pigs for active cutaneous anaphylaxis. only one fraction of calf skin collagen gave a slight, positive reaction. no positive reactions were observed in 21 subjects submitted to patch tests and 12 subjects submitted to scratch tests with pig skin collagen. therefore preparations of pig skin collagen may be used for wound treatment without any risk. | 1976 | 59667 |
| the extensive cross-reaction of several syngeneic rat-anti-bp antiserums with myelin basic proteins (bp) of other species. | | 1976 | 59689 |
| immunochemistry of bovine fibrinogen--ii. analysis by immunoadsorption of the antibody response against three large cyanogen bromide peptides. | | 1976 | 59691 |
| immunological relationships among hexosaminidases of different species. | | 1976 | 59695 |
| recognition of the cattle chromosomes by the q- and g-banding techniques. | | 1976 | 59716 |
| identification of the 1/29-translocation in the swedish red and white (srb) cattle breed by utilization of new staining techniques. | | 1976 | 59717 |
| a novel approach for enzyme histochemical and autoradiographic studies on single cells. | a simple technique that does not involve the use of heat has been developed to fix cells or cell organelles. the cells or organelles are mixed with a bovine serum albumin solution, gelled by the addition of a suitable fixative, and then either embedded or frozen. the gelled mixture contains well preserved cells or organelles that are evenly dispersed, thus eliminating the problems of pellet packing. the technique was excellent for ultrastructural autoradiography where radioactive materials bound ... | 1976 | 59720 |
| a quantitative assay of covalently bound cephalosporin derivatives in cephalosporin-protein conjugates. | | 1976 | 59725 |
| a fragment comprising the last third of bovine serum albumin which accounts for almost all the antigenic reactivity of the native protein. | the fragmentation of native bovine serum albumin by trypsin has been studied in aqueous solution under various conditions with regard to the yield and size of the fragments obtained. from a partial tryptic hydrolysate at ph 8.2 (40 degrees, 1 hour), a homogeneous fragment was isolated in high yield by gel filtration on sephadex g-100, followed by chromatography on deae-cellulose. the molecular weight of the fragment by gel filtration on calibrated sephadex g-100 columns and by sodium dodecyl su ... | 1976 | 59728 |
| modulation of cellular-immune responses in vivo and in vitro by histamine receptor-bearing lymphocytes. | histamine, one of the mediators involved in the ige-mediated reaction, was demonstrated to influence in vivo and in vitro components of cellular-immune reactions in orthochlorbenzoyl-bovine gamma globulin-immune guinea pigs. 10(-3) m histamine reduced by half the size of a delayed hypersensitivity skin test at 24 h. inhibition of skin reactivity by histamine could be partially reversed by h-1 receptor antagonists such as chlorpheniramine and completely prevented by h-2 receptor antagonists such ... | 1976 | 59738 |
| staining technique for definite identification of individual cattle chromosomes in routine analysis. | the purpose of the present communication is to draw attention to the great applicability of a recently introduced r-banding technique for definite identification of cattle chromosomes in routine analysis. banding patterns as well as densitometric measurements of normal bull chromosomes and the so-called 1/29 translocation are demonstrated. | 1976 | 59749 |
| studies on the genetic control of antibody affinity: the independent control of antibody levels and affinity in biozzi mice. | the levels (abt) and relative affinity (kr) of antibody produced to protein antigens injected in saline have been measured in the 22nd generation of the biozzi high (ab/h) and low (ab/l) antibody-producing mice. no significant differences in affinity were observed between the two lines of mice (p 0.10) but the levels of antibody (abt) differed significantly (p 0.0025) when immunized with antigen in saline; however, both ab/h and ab/l mice were able to mount a high affinity response to protein an ... | 1976 | 59780 |
| humoral immune responses in rana catesbiana frogs and tadpoles. | rana catesbiana adult frogs and tadpoles were immunized with the bacteriophage f2, 0x-174, and t4 and the haptens 2,4 dinitrophenyl (dnp) and fluorescein (ftc). the haptens were conjugated with bovine serum albumin (bsa), bovine gamma globulin (bgg), or horsehoe crab hemocyanin (hycn). sera were obtained from immunized animals at invervals up to six months after immunization. the antibody activities were measured by bacteriophage neutralization techniques. sucrose density gradients were used to ... | 1976 | 59790 |
| [relationships between conformation of antigen and specificity of antibody in radioimmunoassay for steroid hormone. ii. synthesis of 6-hydroxytestosterone 6-hemisuccinate-bovine serum albumin conjugates (author's transl)]. | | 1976 | 59803 |
| anaphylactoid reaction to single-component pork insulin. | | 1976 | 59824 |
| effect of bacterial lipopolysaccharides on anti-trinitrophenyl antibody-producing cells. nonspecific modification of the affinity at the cellular level. | the effect of lipopolysaccharide (lps) on anti-trinitrophenyl (tnp) direct plaque-forming cells (pfc) in the spleen of mice and the affinity of antibodies produced by these pfc were examined. simultaneous injection of bacterial lps and tnp-coupled sheep red blood cells(srbc) induced an obvious increase in anti-tnp pfc numbers and heightened the antibody affinity at cellular levels. the higher the doses of lps, the greater the effects. concomitant injection of lps in tnp-coupled homologous mouse ... | 1975 | 59829 |
| characterization of the collagen synthesized by endothelial cells in culture. | [14c]proline and [14c]lysine were incorporated into collagen by cultures of endothelial cells derived from calf aortae. the isomer 3-hydroxy[14c]proline accounted for 10% of the total hydroxy[14c]proline in the collagen isolated from the medium. approximately 81% of the hydroxy[14c]lysine isolated from the medium was glycosylated, and 91% of the glycosylated hydroxy[14c]lysine was in the form of the disaccharide glucosylgalactose. gel filtration chromatography or acrylamide gel electrophoresis i ... | 1976 | 59926 |
| experimental studies on drug sensitization. ii. production of antiacetylsalicylyl antibodies and their detection by passive hemagglutination. | antiacetylsalicylyl antibodies were produced in rabbits and guinea-pigs by innoculation of acetylsalicylic acid incorporated in freund adjuvant. these antibodies were readily detected by a passive hemagglutination test using rabbit erythrocytes incubated with acetylsalicylic acid at alkaline ph. acetylsalicylyl conjugates with human gammaglobulin, bovine gammaglobulin and rabbit gammaglobulin were also prepared by incubating the proteins with acetylsalicylic acid at alkaline ph. in parallel expe ... | 1976 | 59932 |
| comparative in vitro studies of a number of anti-mastitis antibiotics. | in vitro activities of penicillin, ampicillin, dihydrostreptomycin, nafcillin, cloxacillin and commercially available combinations of these antibiotics against a variety of gram-positive mastitis-causing micro-organisms were examined. as a rule, penicillin proved to be more active against penicillin-sensitive strains of micro-organisms than ampicillin and nafcillin and far more so than cloxacillin. in combination with di-hydrostreptomycin, which in itself showed hardly any activiy, the activity ... | 1976 | 59958 |
| the specificity of antisera raised by oestradiol-17beta-3-hemisuccinyl-bovine serum albumin. | the preparation of pure oestradiol-17beta-3-hemisuccinyl-bovine serum albumin conjugate is described. contrary to previous findings this antigen raised reasonably specific antisera in rabbits which possessed a cross reaction of only 2.0% with oestrone, and 0.8% with oestriol. the production of this specific antisera is considered to be due to the high purity of the antigen. the role of the c-3 phenolic hemisuccinyl linkage of the antigen in raising this specific antisera is discussed. | 1976 | 59968 |
| dry period teat seal. | | 1976 | 59999 |
| [normalization of thyroid gland function in cows]. | | 1976 | 60001 |
| production of an equine anti-bovine leukocyte serum. | a method is described for production of an equine anti-bovine leukocyte serum (eabls). leukocytes were harvested from the milk of cow's udders which had been irritated with endotoxin. the washed leukocytes as antigens were administered to 2 horses in a series of subcutaneous and intravenous injections. there was a variably progressive increase in total serum proteins and a decrease in albumin/blobulin ratios, but the most pronounced change was an increase in beta2-globulins. accompanying these c ... | 1976 | 60071 |
| [additive activity of ige and igga anaphylactic antibodies in rats (author's transl)]. | anaphylactic antibodies of two different classes are produced in rats: ige and igga. when injected into the skin of normal animals, ige remains at the site of injection for two weeks or more, while igga diffuses out within hours. in order to determine whether the anaphylactic reaction produced by one class of antibody is modified by the simultaneous presence of the other class of antibody, sites were prepared in normal rats with ige antibody and 24 hours later igga antibody was injected into the ... | 1976 | 60078 |
| kinetics of in vitro bovine keratohyalin synthesis. | a system is described for the study of the in vitro synthesis of bovine keratohyalin for periods of up to 24 h. keratohyalin granules appeared morphologically unaltered during culture, although histochemical stains for rna indicate a markedly diminished rna content by 6 h. incorporation of tritiated histidine began slowly, then became linear between 4 and 24 h in serum-containing media. however, following pre-incubation in serum-free media, increased incorporation occurred from time zero. the in ... | 1976 | 60121 |
| [galactose transfer in the membranes of human milk fat globules (author's transl)]. | galactosyltransferase which catalyzes the transfer from udp-galactose to either endogeneous glycoproteins, free n-acetylglucosamine or n-acetylglucosaminyl residues in the carbohydrate portion of glycoproteins, or to glucose when alpha-lactalbumin is added, occurs in human milk fat globule membranes. various treatments (washing of membranes, freezing and thawing) did not affect this activity. in the presence of triton x-100, the enzyme shows appreciable latency, this detergent was then used to s ... | 1976 | 60134 |
| manganese metabolism in cows and goats. | when 54mncl2 was incubated with fresh bovine or caprine serum for 20 h and the serum subjected to electrophoresis at ph 9.5, the 54mn bound to transferrin and alpha2-macroglobulin in proportions which varied with the temperature of incubation and the temperature of electrophoresis. between 0 and 37 degrees c, the higher the temperature of incubation the larger the proportion bound to transferrin and the lower the proportion bound to alpha2-macroglobulin. the temperature at which electrophoresis ... | 1976 | 60137 |
| [effects of gestagens on the early phases of pregnancy in cattle--clinical experiences. 3. 2 double-blind studies under field conditions: attempt at comparative discussion]. | | 1976 | 60210 |
| inhibition by 5-hydroxytryptamine of anaphylactic histamine release from bovine granulocytes. | a suspension of granulocytes from calves sensitised to horse plasma released histamine when exposed to the antigen. histamine release was strongly inhibited by 5-hydroxytryptamine in a dose-related fashion. 5-ht is known to be released from bovine lungs in vitro, and may regulate the release of histamine in vivo during anaphylaxis. | 1976 | 60245 |
| degradation of encephalitogenic protein in aerobic ascorbic acid solutions. | aerobic ascorbic acid solutions are capable of extensively cleaving the peptide chain of the myelin basic protein. cleavage occurred most readily with c-terminal to aspartic acid, serine, threonine, glutamic acid and leucine residues. | 1976 | 60250 |
| localization of bovine pancreatic polypeptide (bpp)-like immunoreactivity in rat pancreatic monolayer culture. | antiserum to bovine pancreatic polypeptide (bpp) has been used for immunofluorescent staining in the light microscope. with this technique it is possible to detect the presence of specific cells in monolayer culture from neonatal rat pancreas which contain bpp or a closely related peptide. | 1976 | 60252 |
| isolation from peripheral nerve of a protein similar to the glial fibrillary acidic protein. | | 1976 | 60257 |
| influence of bovine tsh on plasma thyroxine levels and thyroid function in brook trout, salvelinus fontinalis (mitchill). | | 1976 | 60268 |
| immunological release of histamine from bovine leucocytes. unusual adrenergic modulation. | sensitized bovine granulocytes release histamine when exposed to specific antigen. in comparison with in vitro systems in several other species, the modulation of this release by adrenergic agents is unique. beta-adrenoceptor stimulation potentiates (rather than inhibits), whereas alpha-adrenoceptor stimulation inhibits (rather than potentiates) histamine release. adrenaline, which is generally considered to be a physiological antagonist of the anaphylactic reaction, potentiated histamine releas ... | 1976 | 60282 |
| analysis of protein-labeling solutions (99m tc) by zonal electrophoresis. | | 1976 | 60295 |
| effect of orally administered duodenal fluid susceptibility of newborn calves to an escherichia coli challenge. | this study was to determine if duodenal contents inoculated orally could alter symptomatic diarrhea produced by escherichia coli [09:k(a)] in neonatal calves. inoculum was obtained 2 h postprandially from duodenal-cannulated milk-fed calves. thirty holstein bull calves obtained within 3 h of birth were assigned alternately to a 3 x 2 factorial experiment. calves received either 1) no e. coli challenge, 2) challenge at 12 h, or 3) challenge at 24 h each with or without 200 ml duodenal fluid orall ... | 1976 | 60352 |
| the surface nature of proteins of a bovine enterovirus, before and after neutralization. | the surface nature of the proteins of a bovine enterovirus have been determined by using 125i and pyridoxal phosphate-sodium borohydride labelling techniques. as found previously, 125i labels only vp1 in intact capsid particles, whereas reaction with pyridoxal phosphate followed by reduction with tritiated sodium borohydride labels vp1, vp2 and vp3. only vp4 is found to have no surface tyrosine, histidine or lysine available for reaction. after neutralization with homologous antisera, however, v ... | 1976 | 60463 |
| mechanism of interaction of myelin basic protein and s-100 protein: metal binding and fluorescence studies. | | 1976 | 60466 |
| letter: immune complexes in kidneys of rats treated with antiserum to luteinising hormone. | | 1976 | 60680 |
| letter: one-hour blood-xylose test in diagnosis of cow's-milk allergy. | | 1976 | 60681 |
| evidence for a gonadotropin from nonpregnant subjects that has physical, immunological, and biological similarities to human chorionic gonadotropin. | substances from urinary extracts of normal, nonpregnant subjects and human pituitary gonadotropin preparations were found to react similarly to human chorionic gonadotropin (hcg) in a radioimmunoassay system that is highly specific for hcg and without crossreactivity to human luteinizing hormone (hlh). the antiserum was produced in a rabbit immunized with a bovine albumin conjugate of the unique carboxyl-terminal peptide (residues 123-145) isolated from a tryptic digest of the reduced, s-carboxy ... | 1976 | 60763 |
| detection of beta2-microglobulin in sera from diverse vertebrate species by the use of a heterologous radioimmunoassay. | the strong cross-reaction between rabbit and human beta2-microglobulins, as detected by goat antisera, has permitted the development of heterologous radioimmunoassays to detect homologues of beta2-microglobulin (beta2mu) in the sera of various vertebrate species. the assay based on the inhibition of the binding reaction between goat anti-rabbit beta2 mu and human beta2 mu divided the sera examined into two groups, one giving high levels of inhibition (approximately 80%). and the other lower leve ... | 1976 | 60779 |
| [effect of temperature and proteolytic enzymes on the immunological properties of 2 acid neurospecific antigens]. | the immunological properties of water-soluble acid neurospecific antigens a and d of the bull brain were studied as affected by heating and proteolytical enzymes (trypsin, mixture of trypsin and alpha-chymotrypsin, protease from streptomyces griseus). the immunological activity of antigen a is not changed essentially after its heating for 20 min at a temperature of 70-80 and 24-hour proteolysis with trypsin. heating at 90-100 c as well as hydrolysis with protease from streptomyces griseus and mi ... | 1976 | 60816 |
| [antigenic analysis and immunological studies of the uterotropic bacterial strains sh6 and 01 isolated from aborted cows]. | the uterotropic strains sh6 and o1 were studied immunoelectrophoretically to establish their antigenic relationship. the more readily diffusing component of the o-antigenic fraction of the strains proved immunologically identical with lpa of the r brucella organisms, while the slower component showed common determinants with the o-antigenic fraction of yersinia pseudotuberculosis. immunodiffusion revealed that the o-antigenic fraction of the o1 strain and lpa of the s brucella organisms contain ... | 1976 | 60821 |
| mixed leukocyte reaction in rats. effect of immunization with bovine encephalitogenic protein and freund's complete adjuvant. | rats of certain strains immunized with bovine encephalitogenic protein (ep) in freund's complete adjuvant develop an impairment of the mixed leukocyte reaction (mlr) similar to that seen in rabbits with experimental autoimmune encephalomyelitis and in humans with certain diseases, including multiple sclerosis. the rats mount a cell-bound response to ep, but encephalomyelitis does not develop. the component causing the impairment was analysed in a culture system using inbred rat strains and f1 hy ... | 1976 | 60845 |
| [demonstration of immune complex components in kidney glomeruli in bovine virus diarrhea mucosal disease]. | | 1976 | 60850 |
| calcium and stimulus secretion coupling in the neurohypophysis. v. the effects of the ca2+ ionophores a23187 and x537 a on vasopressin release and 45ca2+ efflux; interactions with sodium and a verapamil analogue (d600). | slices from ox neurohypophyses were incubated in a calcium-free medium with the ionophores a23187 or x537a. x537a (5 x 10(-5) mol/l) caused a marked release of vasopressin, neurophysin and protein to the medium. a23187 (2 x 10(-5) mol/l) did not cause any release by itself, but when ca2+ was added to the medium in the presence of the ionophore, an increase in the release of vasopressin, neuorphysin and protein occurred. release of lactate dehydrogenase and peptidase were not affected by the iono ... | 1976 | 60866 |
| insecticides in human breast milk. | fifty samples of human breast milk were analysed by gas chromatography and thin layer chromatography for ddt (dichlordiphenyltrichloraethan), hexachlorobenzol, benezene-hexachlorides, dieldrin, aldrin and heptachlorepoxide. the three first substances were found in all samples in amounts varying from quite small up to eleven times the who recommended maximum for cow milk. the other substances were found in fewer milk samples, however, in some of these samples they were found in relatively high am ... | 1976 | 60871 |
| release of active substances during anaphylaxis. | | 1976 | 60877 |
| evaluation of bovine spermatozoal morphologic features after staining or fixation. | two experiments were conducted to evaluate effects of 3 stains and 2 fixatives on morphologic features of bovine spermatozoa. in experiment 1, the morphologic features of acrosomes of raw and incubated, extended spermatozoa were evaluated after staining with hancock's blom's or wells-awa's stains or after fixation with buffered glutaraldehyde. evaluations were done of stained smears by bright field microscopy and of fixed, unstained preparations, by differential interference contrast microscopy, ... | 1976 | 60893 |
| inhibition of specific binding of dnp (dinitrophenyl) determinant to lymphoid-cell receptors by related and unrelated compounds : quantitative studies in vitro. | the effect of related and unrelated compounds on the specific binding of dinitrophenyl-coupled bacteriophage (dnp-t4) to lymphoid cell receptors has been examined and compared with the effect on the neutralization of dnp-t4 by anti-dnp serum. spleen cells and sera from balb/c mice immunized with dnp-bovine serum albumin were used. the binding of dnp-t4 to the cells was inhibited by dnp-eacp, di-dnp-lys, dnp-tyr, dnp-p(ornith) and dnp-bsa (among the dnp-derivatives tested), tnp-bsa, ars-p(tyr) an ... | 1976 | 60907 |
| development of immuneeoglobulin and antibody-forming cells in different stages of the immun response. | after primary immunization of mice, rats and rabbits with antigens (horse radish peroxidase, bovine serum albumin and muchroom tyrosinase) emulsified in complete or incomplete freund's adjuvant, both cells synthesizing immunoglobulin without detectable antibody function and antibody-producing cells were detected. the first cells which appeared were synthesizing and secreting igg and igm immunoglobulins without antibody function. these cells were progressively replaced by cells synthesizing and s ... | 1976 | 60908 |
| [proceedings: bovine thyroid ribonuclease (type iv)]. | | 1976 | 60923 |
| study of messenger-like rna extracted from hela cells polysomes. ii. identification of the types of rna eluted from methylated albumin kieselguhr columns. | together with the elution pattern of pure messenger rna molecules of various origin, the labelling kinetics of rapidly labelled heterogeneously sedimenting rna (hsrna) extracted from polysomes of hela cells have been studied by chromatogrphy on columns made of methylated bovine serum albumin adsorbed on kieselguhr. hsrna is eluted within three peaks-ip, q2p and tdp-following in that order the increase of nac1 concentration in the eluting buffer. besides peak tdp which results from an experiment ... | 1976 | 60930 |
| [proceedings: phospholipase a1, phospholipase a2 and lipase activities in bovine thyroid mitochondrial fractions]. | | 1976 | 60936 |
| proceedings: activation characteristics of phosphorylase phosphatase preparations from bovine adrenal cortex after ethanol treatment. | | 1976 | 60954 |
| [proceedings: lipid composition of bovine thyroid gland. vi permethylation and periodate oxidation studies of neutral glycolipids]. | | 1976 | 60961 |
| [proceedings: lipid composition of bovine thyroid gland. vii. anomer configuration of glycoside bonds in neutral glycolipids]. | | 1976 | 60962 |
| influence of the alpha-globulin fraction isolated from calf serum on graft-versus-host reaction in strongly histoincompatible mice. | the influence of a two alpha-globulin fractions isolated from calf serum by the method of mowbray on the graft-v-host reaction (gvh) in the f1 hybrids of inbred mouse strains differing in h-2 locus after administration of parental lymphatic cells was studied. some of the mice were x-irradiated with 500 r before administration of parental cells. the studied fractions exerted strong suppressive action on the splenomegalic reaction and proliferative phenomena in the spleen, accompanied by intensif ... | 1976 | 60985 |
| further studies on the influence of calf alpha-globulin on survival of skin allografts in mice. | a single injection of 10 mg of alpha-globulin fraction isolated from calf serum prolonged survival of skin allografts in mice provided the preparation was injected two days before grafting. an immunosuppressive effect was also obtained by injecting alpha-globulin in a total dose of 13 mg in the second week after grafting in mice pretreated with antithymocyte serum; on the other hand, such an effect was not obtained when alpha-globulin was injected in the early days after grafting in doses of up ... | 1976 | 60986 |
| effect of ionophores on intralysosomal ph. | 1. the effect of ionophores on the intralysosomal ph (as estimated from the distribution of a weak acid or base), on the distribution of 42k+ across the lysosomal membrane, and on the intralysosomal degradation of 125i-labelled bovine serum albumin has been studied. 2. nigericin and x537a equilibrate both 42k+ and h+ across the lysosomal membrane. gramicidin equilibrates h+ across the lysosomal membrane, this equilibration being more effective in a nacl than in a kcl medium. thus all three ionop ... | 1976 | 61045 |
| [antigen specific to spermatozoal cell membrane and male sterility--isolation of specific antigen]. | | 1976 | 61084 |
| [some aspects of rabies in the 18th century]. | | 1976 | 61094 |