| conidial vigor vs. viability as predictors of virulence of entomopathogenic fungi. | we tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring>16h to germinate) are less virulent than vigorous conidia exhibiting fast germination (requiring⩽16h to germinate). preparations of beauveria bassiana s.l. strain cg 1027 with variable ratios of vigorous to debilitated conidia were assayed against third-instar larvae of spodoptera frugiperda. as the proportion of debilitated conidia in test preparations increased, lc50 expressed in terms of total viable coni ... | 2015 | 25573792 |
| recombinant pestivirus e2 glycoproteins prevent viral attachment to permissive and non permissive cells with different efficiency. | bovine viral diarrhoea virus (bvdv) is an economically important animal pathogen, which like other pestiviruses has similar molecular biological features to hepaciviruses, including human hepatitis c virus. the pestivirus e2 glycoproteins are the major target for virus-neutralising antibodies, as well as playing a role in receptor binding and host range restriction. in this study, recombinant e2 glycoproteins (re2) derived from three different pestivirus species were examined for their inhibitor ... | 2014 | 24874197 |
| using bicistronic baculovirus expression vector system to screen the compounds that interfere with the infection of chikungunya virus. | chikungunya virus (chikv) is the etiologic agent of chikungunya fever and has emerged in many countries over the past decade. there are no effective drugs for controlling the disease. a bicistronic baculovirus expression system was utilized to co-express chikv structural proteins c (capsid), e2 and e1 and the enhanced green fluorescence protein (egfp) in spodoptera frugiperda insect cells (sf21). the egfp-positive sf21 cells fused with each other and with uninfected cells to form a syncytium is ... | 2016 | 27233279 |
| expression and purification of e2 glycoprotein from insect cells (sf9) for use in serology. | chikungunya virus (chikv) is a mosquito-borne arbovirus which poses a major threat to global public health. definitive chikv diagnosis is crucial, especially in distinguishing the disease from dengue virus, which co-circulates in endemic areas and shares the same mosquito vectors. laboratory diagnosis is mainly based on serological or molecular approaches. the e2 glycoprotein is a good candidate for serological diagnosis since it is the immunodominant antigen during the course of infection, and ... | 2016 | 27233260 |
| anti-influenza a virus activity of uridine derivatives of 2-deoxy sugars. | influenza viruses are important pathogens that cause respiratory infections in humans and animals. apart from vaccinations, antiviral drugs play a significant role in controlling spread of the disease. influenza a virus contains two membrane glycoproteins on the external part of viral envelope: hemagglutinin (ha) and neuraminidase (na), which are crucial for productive infection in target cells. in the present work, two derivatives of tunicamycin - uridine derivatives of 2-deoxy sugars (designat ... | 2013 | 23911991 |
| genome analysis of heliothis virescens ascovirus 3h isolated from china. | no ascovirus isolated from china has been sequenced so far. therefore, in this study, we aimed to sequence the genome of heliothis virescens ascovirus 3h (hvav-3h) using the 454 pyrosequencing technology. the genome was found to be 190,519-bp long with a g+c content of 45.5%. we also found that it encodes 185 hypothetical open reading frames (orfs) along with at least 50 amino acids, including 181 orfs found in other ascoviruses and 4 unique orfs. gene-parity plots and phylogenetic analysis reve ... | 2017 | 28382574 |
| analysis of the activity of virus internal ribosome entry site in silkworm bombyx mori. | internal ribosome entry site (ires) has been widely used in genetic engineering; however, the application in silkworm (bombyx mori) has hardly been reported. in this study, the biological activity of partial sequence of encephalomyocarditis virus (emcv) ires, rhopalosiphum padi virus (rhpv) ires, and the hybrid of ires of emcv and rhpv were investigated in spodoptera frugiperda (sf9) cell line and silkworm tissues. the hybrid ires of emcv and rhpv showed more effective than emcv ires or rhpv ire ... | 2013 | 23644592 |
| bioactivity of the essential oil of an argentine collection of acanthospermum hispidum (asteraceae). | the essential oil of an argentine collection of the annual herb acanthospermum hispidum dc. (asteraceae), which contains the sesquiterpenoids beta-caryophyllene (35.2%), alpha-bisabolol (11.4%), and germacrene d (11.1%) as major constituents, displayed molluscicidal effects on an adult population of biomphalaria peregrina orbigny (ld50 37.8 microg/ml) and produced alteration of the feeding and oviposition behavior of the polyphagous insect spodoptera frugiperda smith when incorporated in the lar ... | 2012 | 22474971 |
| tracking the virus-like particles of macrobrachium rosenbergii nodavirus in insect cells. | macrobrachium rosenbergii nodavirus (mrnv) poses a major threat to the prawn industry. currently, no effective vaccine and treatment are available to prevent the spread of mrnv. its infection mechanism and localisation in a host cell are also not well characterised. the mrnv capsid protein (mrnvc) produced in escherichia coli self-assembled into virus-like particles (vlps) resembling the native virus. thus, fluorescein labelled mrnvc vlps were employed as a model to study the virus entry and loc ... | 2017 | 28194311 |
| virus-like particle of macrobrachium rosenbergii nodavirus produced in spodoptera frugiperda (sf9) cells is distinctive from that produced in escherichia coli. | macrobrachium rosenbergii nodavirus (mrnv) is a virus native to giant freshwater prawn. recombinant mrnv capsid protein has been produced in escherichia coli, which self-assembled into virus-like particles (vlps). however, this recombinant protein is unstable, degrading and forming heterogenous vlps. in this study, mrnv capsid protein was produced in insect spodoptera frugiperda (sf9) cells through a baculovirus system. dynamic light scattering (dls) and transmission electron microscopy (tem) re ... | 2016 | 27860432 |
| expression and purification of epha2 tyrosine kinase domain for crystallographic and nmr studies. | the receptor tyrosine kinase epha2 is overexpressed in several cancers (breast, head and neck, non-small-cell lung cancer). small-molecule-based inhibition of the epha2 kinase domain (kd) is seen as an important strategy for therapeutic intervention. however, obtaining structural information by crystallography or nmr spectroscopy for drug discovery is severely hampered by the lack of pure, homogeneous protein. here, different fragments of the epha2 kd were expressed and purified from both bacter ... | 2016 | 27685543 |
| cell-free eukaryotic systems for the production, engineering, and modification of scfv antibody fragments. | open cell-free translation systems based on escherichia coli cell lysates have successfully been used to produce antibodies and antibody fragments. in this study, we demonstrate the cell-free expression of functional single-chain antibody variable fragments (scfvs) in a eukaryotic and endotoxin-free in vitro translation system based on spodoptera frugiperda (sf21) insect cell extracts. three scfv candidates with different specificities were chosen as models. the first scfv candidate sh527-iia4 s ... | 2014 | 25821419 |
| cloning and functional expression of a chitinase cdna from the apple leaf miner moth lithocolletis ringoniella. | insect chitinase plays essential roles in chitin catabolism involved in digestion and molting during insect development. in the current work, we cloned a chitinase cdna, lrcht5, from the apple leaf miner moth lithocolletis ringoniella and characterized its amino acid sequence and protein properties. the l. ringoniella chitinase cdna was 2136 bp in length with an open reading frame of 1737 bp that encodes a polypeptide of 579 amino acid residues with a predicted molecular mass of 64.4 kda and pi ... | 2015 | 25756539 |
| [expression, purification and characterization of catalytic domain of protein kinase d1 in baculovirus-insect cell expression system]. | protein kinase d (pkd) is a novel family of serine/threonine kinases and diacylglycerol (dag) receptors and has been documented in a variety of cellular processes. to get high purity catalytic domain of pkd1 (pkd1-cat) for crystallography study, the gst-tagged pkd1-cat gene was cloned into a baculovirus transfer vector pfastbac1 (donor plasmid). when the recombinant plasmid was transformed into dh10bac competent escherichia coli, which contains a baculovirus shuttle vector (bacmid), transpositio ... | 2014 | 25507482 |
| [expression, purification and characterization of catalytic domain of protein kinase d1 in baculovirus-insect cell expression system]. | protein kinase d (pkd) is a novel family of serine/threonine kinases and diacylglycerol (dag) receptors and has been documented in a variety of cellular processes. to get high purity catalytic domain of pkd1 (pkd1-cat) for crystallography study, the gst-tagged pkd1-cat gene was cloned into a baculovirus transfer vector pfastbac1 (donor plasmid). when the recombinant plasmid was transformed into dh10bac competent escherichia coli, which contains a baculovirus shuttle vector (bacmid), transpositio ... | 2014 | 25423759 |
| robust and low cost uniform (15)n-labeling of proteins expressed in drosophila s2 cells and spodoptera frugiperda sf9 cells for nmr applications. | nuclear magnetic resonance spectroscopy is a powerful tool to study structural and functional properties of proteins, provided that they can be enriched in stable isotopes such as (15)n, (13)c and (2)h. this is usually easy and inexpensive when the proteins are expressed in escherichiacoli, but many eukaryotic (human in particular) proteins cannot be produced this way. an alternative is to express them in insect cells. labeled insect cell growth media are commercially available but at prohibitiv ... | 2014 | 25172991 |
| liposome reconstitution and modulation of recombinant prenylated human rac1 by gefs, gdi1 and pak1. | small rho gtpases are well known to regulate a variety of cellular processes by acting as molecular switches. the regulatory function of rho gtpases is critically dependent on their posttranslational modification at the carboxyl terminus by isoprenylation and association with proper cellular membranes. despite numerous studies, the mechanisms of recycling and functional integration of rho gtpases at the biological membranes are largely unclear. in this study, prenylated human rac1, a prominent m ... | 2014 | 25014207 |
| bmice-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, bombyx mori. | in this study we identified a potential pro-apoptotic caspase gene, bombyx mori(b. mori)ice-2 (bmice-2) which encoded a polypeptide of 284 amino acid residues, including a (169)qacrg(173) sequence which surrounded the catalytic site and contained a p20 and a p10 domain. bmice-2 expressed in escherichia coli (e. coli) exhibited high proteolytic activity for the synthetic human initiator caspase-9 substrates ac-lehd-pna, but little activity towards the effector caspase-3 substrates ac-devd-pna. wh ... | 2014 | 24491540 |
| production and biomedical applications of virus-like particles derived from polyomaviruses. | virus-like particles (vlps), aggregates of capsid proteins devoid of viral genetic material, show great promise in the fields of vaccine development and gene therapy. these particles spontaneously self-assemble after heterologous expression of viral structural proteins. this review will focus on the use of virus-like particles derived from polyomavirus capsid proteins. since their first recombinant production 27 years ago these particles have been investigated for a myriad of biomedical applicat ... | 2013 | 23999392 |
| expression of grape class iv chitinase in spodoptera frugiperda (sf9) insect cells. | most of pathogenesis related (pr) proteins possess complicated structures; hence their active recombinant forms are usually produced in eukaryotic systems. in this study, we employed an insect cell line to express a recombinant form of a previously identified grape pr3 allergen categorised as class iv chitinase. | 2016 | 23481555 |
| expression of recombinant flavin-containing monooxygenases in a baculovirus/insect cell system. | the baculovirus/insect cell heterologous expression system provides an important tool for investigating the catalytic activity of individual drug-metabolizing enzymes toward a particular substrate. in this chapter we describe a baculovirus/insect cell system that we have used for the expression of human and mouse flavin-containing monooxygenases. methods are described for the generation of recombinant baculoviral dnas, via both site-specific transposition in escherichia coli and site-specific re ... | 2006 | 27699678 |
| enhanced pest resistance of maize leaves expressing monocot crop plant-derived ribosome-inactivating protein and agglutinin. | although many insect resistance genes have been identified, the number of studies examining their effects in combination using transgenic systems is limited. this study introduced a construct into maize containing the coding sequence for maize ribosome-inactivating protein (mrip) and wheat germ agglutinin (wga). many transformants produced both the mrip and wga in leaves. mature leaves expressing higher levels of these two proteins were more resistant to feeding by first-instar larvae of fall ar ... | 2012 | 23078237 |
| a baculovirus-mediated strategy for full-length plant virus coat protein expression and purification. | garlic production is severely affected by virus infection, causing a decrease in productivity and quality. there are no virus-free cultivars and garlic-infecting viruses are difficult to purify, which make specific antibody production very laborious. since high quality antisera against plant viruses are important tools for serological detection, we have developed a method to express and purify full-length plant virus coat proteins using baculovirus expression system and insects as bioreactors. | 2013 | 23945471 |
| insect cell surface expression of hemagglutinin (ha) of egyptian h5n1 avian influenza virus under transcriptional control of whispovirus immediate early-1 promoter. | in the present study, whispovirus immediate early 1 promoter (ie-1) was used to initiate surface expression of the hemagglutinin (ha) protein of egyptian h5n1 avian influenza virus (aiv) by using the baculovirus expression vector system. the ha gene and whispovirus ie-1 promoter sequence were synthesized as a fused expression cassette (ie1-ha) and successfully cloned into the pfastbac-1 transfer vector. the recombinant vector was transformed into dh10bac competent cells, and the recombinant bacm ... | 2014 | 25112319 |
| development of influenza h7n9 virus like particle (vlp) vaccine: homologous a/anhui/1/2013 (h7n9) protection and heterologous a/chicken/jalisco/cpa1/2012 (h7n3) cross-protection in vaccinated mice challenged with h7n9 virus. | the recent emergence of severe human illness caused by avian-origin influenza a(h7n9) viruses in china has precipitated a global effort to rapidly develop and test vaccine candidates. to date, non-a(h7n9) h7 subtype influenza vaccine candidates have been poorly immunogenic and difficulties in production of a(h7n9) virus seed strains have been encountered. a candidate recombinant a(h7n9) vaccine consisting of full length, unmodified hemagglutinin (ha) and neuraminidase (na) from the a/anhui/1/201 ... | 2013 | 23891795 |
| meeting report vlpnpv: session 6: development and characterization. | | 2014 | 25458600 |
| binding and entry of a non-enveloped t=4 insect rna virus is triggered by alkaline ph. | tetraviruses are small, non-enveloped, rna viruses that exclusively infect lepidopteran insects. their particles comprise 240 copies of a single capsid protein precursor (cp), which undergoes autoproteolytic cleavage during maturation. the molecular mechanisms of capsid assembly and maturation are well understood, but little is known about the viral infectious lifecycle due to a lack of tissue culture cell lines that are susceptible to tetravirus infection. we show here that binding and entry of ... | 2016 | 27614703 |
| permanent genetic resources added to molecular ecology resources database 1 december 2011-31 january 2012. | this article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: barteria fistulosa, bombus morio, galaxias platei, hematodinium perezi, macrocentrus cingulum brischke (a.k.a. m. abdominalis fab., m. grandii goidanich or m. gifuensis ashmead), micropogonias furnieri, nerita melanotragus, nilaparvata lugens stål, sciaenops ocellat ... | 2012 | 22448966 |
| expression of recombinant rotavirus proteins harboring antigenic epitopes of the hepatitis a virus polyprotein in insect cells. | rotavirus and hepatitis a virus (hav) spread by the fecal-oral route and infections are important in public health, especially in developing countries. here, two antigenic epitopes of the hav polyprotein, domain 2 (d2) and domain 3 (d3), were recombined with rotavirus vp7, generating d2/vp7 and d3/vp7, cloned in a baculovirus expression system, and expressed in spodoptera frugiperda 9 (sf9) insect cells. all were highly expressed, with peak expression 2 days post-infection. western blotting and ... | 2012 | 24130930 |
| comparative proteomics analysis of apoptotic spodoptera frugiperda cells during p35 knockout autographa californica multiple nucleopolyhedrovirus infection. | infection with autographa californica multiple nucleopolyhedrovirus (acmnpv) mutants lacking a functional p35 gene can induce host cell apoptosis, which provides the possibility to use the potential of these viruses in the biological control of pest insects. nonetheless, the proteomics or the protein changes of spodoptera frugiperda (sf9) cells infected with p35 knockout acmnpv have not yet been studied. to further improve the use of acmnpv, we set out to analyze the protein composition and prot ... | 2016 | 26922645 |
| fungal dna virus infects a mycophagous insect and utilizes it as a transmission vector. | mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically via sexual/asexual spores. previously, we reported that a gemycircularvirus, sclerotinia sclerotiorum hypovirulence-associated dna virus 1 (sshadv-1), could infect its fungal host extracellularly. here, we discovered that sshadv-1 could infect a mycophagous insect, lycoriella ingenua, and use it as a transmission vector. virus acquired by larvae feeding on colonies of a virus-infected strain of s. sclerotiorum ... | 2016 | 27791095 |
| chimaeric vp2 proteins from infectious bursal disease virus containing the n-terminal m2e of h9 subtype avian influenza virus induce neutralizing antibody responses to both viruses. | subunit vaccines capable of inducing antibody against both infectious bursal disease virus (ibdv) and h9 subtype avian influenza virus (aiv) were developed. the vp2 protein of ibdv was used as a cargo protein to display a 12-amino-acid immunodominant epitope derived from the n-terminal m2 extracellular domain (nm2e) of the h9 subtype aiv. two chimaeric proteins were constructed by insertion of one copy of the nm2e into the pbc region (vp2bcnm2e(h9)) or by fusing four copies of nm2e to the carbox ... | 2013 | 23607544 |
| enhanced insecticidal activity of chilo iridescent virus expressing an insect specific neurotoxin. | previously we have generated a recombinant chilo iridescent virus (civ) by inserting the green fluorescent protein gene (gfp) into the civ 157l open reading frame (orf) locus and showed that this recombinant (rciv-δ157l-gfp) was fully infectious both in cell culture as well as in insect larvae. this study opened up a new avenue for increasing the speed of kill of civ and other iridoviruses by inserting virulence or toxin genes into the viral genome. in the current study we constructed a recombin ... | 2016 | 27369385 |
| production of japanese encephalitis virus-like particles using the baculovirus-insect cell system. | the production of a secreted form of japanese encephalitis (je) virus-like particles (vlps) using the baculovirus-insect cell system was investigated. a recombinant baculovirus that contained the je virus (jev) prm signal sequence and the genes encoding the precursor (prm) of the viral membrane protein (m) and the envelope glycoprotein (e) was constructed. western blotting and enzyme-linked immunosorbent assay (elisa) of the culture supernatant showed that spodoptera frugiperda sf9 cells infecte ... | 2012 | 22841798 |
| preparative-cum-quantitative mass-directed analysis of swainsonine and its in situ activity against sf-21 cell line. | swainsonine is a polyhydroxy indolizidine alkaloid with various research and potential therapeutic applications. in this work, swainsonine was partially purified (2.5-folds) with acetone-methanol solvent system from metarhizium anisopliae fermentation broth. the partially purified broth was further subjected to mass-directed preparative-cum-quantitative analysis. swainsonine was eluted as ms1 fraction [m + h](+) 174.36 ± 0.21 at 4.91 ± 0.04 min with calculated yield of 7.85 ± 1.59 μg ml(-1) corr ... | 2013 | 23841458 |
| preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of newcastle disease virus. | a recombinant hemagglutinin-neuraminidase (rhn) protein from newcastle disease virus (ndv) with hemagglutination (ha) activity was expressed in spodoptera frugiperda cells using a baculovirus expression system. the rhn protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (hi) test for the detection and titration of ndv-specific antibodies present in chicken sera. the rhn antigen produced high ha titers of 2(13) per 25 μl, which were similar to those of th ... | 2013 | 23820164 |
| fall armyworm-associated gut bacteria modulate plant defense responses. | mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid-mediated defense responses in plants. this study investigated the effects of bacteria from oral secretions of the fall armyworm spodoptera frugiperda on herbivore-induced defenses in tomato and maize plants. using culture-dependent methods, we identified seven different bacterial isolates belonging to the family enterobacteriacea from the oral secretions ... | 2017 | 28027025 |
| haenamindole and fumiquinazoline analogs from a fungicolous isolate of penicillium lanosum. | three amino acid-derived compounds, haenamindole (1) and 2'-epi-fumiquinazolines c (2) and d (3), were isolated from cultures of a fungicolous isolate of penicillium lanosum (myc-1813=nrrl 66231). compound 1 was also encountered in cultures of p. corylophilum (myc-418=nrrl 28126). structure elucidation of these metabolites was based mainly on high resolution mass spectrometry and nmr data analysis. haenamindole (1) was found to be a recently reported diketopiperazine-type metabolite that incorpo ... | 2016 | 27328870 |
| role of rice stripe virus nsvc4 in cell-to-cell movement and symptom development in nicotiana benthamiana. | our previous work has demonstrated that the nsvc4 protein of rice stripe virus (rsv) functions as a cell-to-cell movement protein. however, the mechanisms whereby rsv traffics through plasmodesmata (pd) are unknown. here we provide evidence that the nsvc4 moves on the actin filament and endoplasmic reticulum network, but not microtubules, to reach cell wall pd. disruption of cytoskeleton using different inhibitors altered nsvc4 localization to pd, thus impeding rsv infection of nicotiana bentham ... | 2012 | 23233857 |
| pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications. | pyocyanin is a redox active phenazine pigment produced by pseudomonas aeruginosa, with broad antibiotic activity having pharmacological, aquaculture, agriculture and industrial applications. in the present work cytotoxicity induced by pyocyanin is demonstrated in a human embryonic lung epithelial cell line (l-132), a rainbow trout gonad cell line (rtg-2) and a spodoptera frugiperda pupal ovarian cell line (sf9). for toxicity evaluation, cellular morphology, mitochondrial function (xtt), membrane ... | 2016 | 25091858 |
| codon optimization of the rabbit hemorrhagic disease virus (rhdv) capsid gene leads to increased gene expression in spodoptera frugiperda 9 (sf9) cells. | rabbit hemorrhagic disease (rhd) is contagious and highly lethal. commercial vaccines against rhd are produced from the livers of experimentally infected rabbits. although several groups have reported that recombinant subunit vaccines against rabbit hemorrhagic disease virus (rhdv) are promising, application of the vaccines has been restricted due to high production costs or low yield. in the present study, we performed codon optimization of the capsid gene to increase the number of preference c ... | 2013 | 23820215 |
| insect cell entrapment, growth and recovering using a single-use fixed-bed bioreactor. scaling up and recombinant protein production. | insect cells are largely used for industrial production of vaccines, viral vectors and recombinant proteins as well as in research and development as an important tool for biology and bioprocess studies. they grow in suspension and are semi-adherent cells. among the cell culture systems enabling scalable bioprocess the single-use fixed-bed icellis(®) bioreactors offer great advantages. we have established the conditions for drosophila melanogaster schneider 2 (s2) and spodoptera frugiperda (sf9) ... | 2015 | 26481831 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| non-structural protein p6 encoded by rice black-streaked dwarf virus is recruited to viral inclusion bodies by binding to the viroplasm matrix protein p9-1. | like other members of the family reoviridae, rice black-streaked dwarf virus (rbsdv, genus fijivirus) is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms. rbsdv p9-1 is the key protein for the formation of viroplasms, but little is known about the other proteins of the viroplasm or the molecular interactions amongst its components. rbsdv non-structural proteins were screened for their association with p9-1 using a co-immunoprecipitation assa ... | 2013 | 23636822 |
| downstream processing of virus-like particles: single-stage and multi-stage aqueous two-phase extraction. | the demand for vaccines against untreated diseases has enforced the research and development of virus-like particle (vlp) based vaccine candidates in recent years. significant progress has been made in increasing vlp titres during upstream processing in bacteria, yeast and insect cells. considering downstream processing, the separation of host cell impurities is predominantly achieved by time-intensive ultracentrifugation processes or numerous chromatography and filtration steps. in this work, w ... | 2015 | 25637013 |
| cell-penetrating recombinant peptides for potential use in agricultural pest control applications. | several important areas of interest intersect in a class of peptides characterized by their highly cationic and partly hydrophobic structure. these molecules have been called cell-penetrating peptides (cpps) because they possess the ability to translocate across cell membranes. this ability makes these peptides attractive candidates for delivery of therapeutic compounds, especially to the interior of cells. compounds with characteristics similar to cpps and that, in addition, have antimicrobial ... | 2012 | 24281256 |
| pathogenicity of isolates of serratia marcescens towards larvae of the scarab phyllophaga blanchardi (coleoptera). | serratia marcescens is a gram negative bacterium (enterobacteriaceae) often associated with infection of insects. in order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased phyllophaga spp (coleoptera:scarabaeidae) larvae collected from cornfields in mexico. five isolates were identified as serratia marcescens by 16s rrna gene sequencing and biochemical tests. oral and injection bioassays using healthy p ... | 2015 | 25984910 |
| sv40 in vitro packaging: a pseudovirion gene delivery system. | nuclear extracts of spodoptera frugiperda (sf9) insect cells infected with baculoviruses encode the simian virus 40 (sv40) major coat protein (vp1). as described in this protocol, these extracts are able to package supercoiled plasmid dna or rna interference (rnai) sequences in the presence of mgcl(2), cacl(2), and atp, thus forming sv40 pseudovirions in vitro. such packaging has numerous advantages over other viral and nonviral delivery systems. specifically, it provides a wide host range and h ... | 2012 | 22949719 |
| the sf32 unique gene of spodoptera frugiperda multiple nucleopolyhedrovirus (sfmnpv) is a non-essential gene that could be involved in nucleocapsid organization in occlusion-derived virions. | a recombinant virus lacking the sf32 gene (sf32null), unique to the spodoptera frugiperda multiple nucleopolyhedrovirus (sfmnpv), was generated by homologous recombination from a bacmid comprising the complete viral genome (sfbac). transcriptional analysis revealed that sf32 is an early gene. occlusion bodies (obs) of sf32null contained 62% more genomic dna than viruses containing the sf32 gene, sfbac and sf32null-repair, although sf32null dna was three-fold less infective when injected in vivo. ... | 2013 | 24204916 |
| first study of different insect cells to triatoma virus infection. | the use of viruses for biological control is a new option to be considered. the family dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. the triatoma virus (trv), a member of this family, affects triatomine transmitters of chagas disease, which is endemic in latin america but also expanding its worldwide distribution. to this end, we attempted virus replication in diptera, aedes albopictus (clone c6/36) and lepidoptera spodoptera ... | 2015 | 25481388 |
| chimaeric virus-like particles derived from consensus genome sequences of human rotavirus strains co-circulating in africa. | rotavirus virus-like particles (rv-vlps) are potential alternative non-live vaccine candidates due to their high immunogenicity. they mimic the natural conformation of native viral proteins but cannot replicate because they do not contain genomic material which makes them safe. to date, most rv-vlps have been derived from cell culture adapted strains or common g1 and g3 rotaviruses that have been circulating in communities for some time. in this study, chimaeric rv-vlps were generated from the c ... | 2014 | 25268783 |
| the silencing suppressor (nss) protein of the plant virus tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects. | in this work, we showed that cell death induced by a recombinant (vacnss) autographa californica multiple nucleopolyhedrovirus (acmnpv) expressing the silencing suppressor (nss) protein of tomato spotted wilt virus (tswv) was enhanced on permissive and semipermissive cell lines. the expression of a heterologous gene (firefly luciferase) during co-infection of insect cells with vacnss and a second recombinant baculovirus (vagppolhfluc) was shown to increase when compared to single vagppolhfluc in ... | 2015 | 26323262 |
| nanostructural characterization of sf9 cells during virus-like particles generation. | sf9 cells (a clonal isolate of spodoptera frugiperda sf21 cells) are commonly used to generate recombinant virus-like particles (vlps). for vlps generation, sf9 cells are infected with recombinant baculoviruses (rbv) expressing desired proteins. during rbv infections, sf9 cells have changes in cell diameters and surface structures. in this study, for the first time, we investigated nanostructural changes of sf9 cells using atomic force microscopy (afm) during vlps generation containing toxoplasm ... | 2016 | 27111226 |
| structure and bioactivity of a modified peptide derived from the lps-binding domain of an anti-lipopolysaccharide factor (alf) of shrimp. | the lipopolysaccharide binding domain (lbd) in anti-lipopolysaccharide factor (alf) is the main functional element of alf, which exhibits antimicrobial activities. our previous studies show that the peptide lbdv, synthesized based on the modified sequence of lbd (named lbd2) from fcalf2, exhibited an apparently enhanced antimicrobial activity. to learn the prospect of lbdv application, the characteristics of lbdv were analyzed in the present study. the lbdv peptide showed higher antimicrobial an ... | 2016 | 27213409 |
| cecropins as a marker of spodoptera frugiperda immunosuppression during entomopathogenic bacterial challenge. | an antimicrobial peptide (amp) of the cecropin family was isolated by hplc from plasma of the insect pest, spodoptera frugiperda. its molecular mass is 3910.9 da as determined by mass spectrometry. thanks to the est database spodobase, we were able to describe 13 cdnas encoding six different cecropins which belong to the sub-families ceca, cecb, cecc and cecd. the purified peptide identified as cecb1 was chemically synthesized (sycecb1). it was shown to be active against gram-positive and gram-n ... | 2012 | 22487443 |
| chitosan and its quaternized derivative as effective long dsrna carriers targeting shrimp virus in spodoptera frugiperda 9 cells. | rna interference (rnai) is a promising strategy to combat shrimp viral pathogens at lab-scale experiments. development of effective orally delivered agents for double-stranded (ds)rna is necessary for rnai application at farm level. since continuous shrimp cell lines have not been established, we are developing a dsrna-delivery system in spodoptera frugiperda (sf9) cells for studying in vitro rnai-mediated gene silencing of shrimp virus. sf9 cells challenged with yellow head virus (yhv) were use ... | 2012 | 22575788 |
| evaluation of corn germplasm lines for multiple ear-colonizing insect and disease resistance. | ear-colonizing insects and diseases that reduce yield and impose health threats by mycotoxin contaminations in the grain, are critical impediments for corn (zea mays l.) production in the southern united states. ten germplasm lines from the germplasm enhancement of maize (gem) program in ames, ia, and raleigh, nc, and 10 lines (derived from gem germplasm) from the texas agricultural experiment station in lubbock, tx, were examined in 2007 and 2008 with local resistant and susceptible controls. f ... | 2012 | 22928329 |
| conspecific and heterospecific aboveground herbivory both reduce preference by a belowground herbivore. | insect herbivores damage plants both above- and belowground, and interactions in each realm can influence the other via shared hosts. while effects of leaf damage on aboveground interactions have been well-documented, studies examining leaf damage effects on belowground interactions are limited, and mechanisms for these indirect interactions are poorly understood. we examined how leaf herbivory affects preference of root-feeding larvae [acalymma vittatum f. (coleoptera: chrysomelidae)] in cucumb ... | 2015 | 26313185 |
| extractives of seeds of the meliaceae: effects onspodoptera frugiperda (j.e. smith),acalymma vittatum (f.), andartemia salina leach. | hexane and ethanol extracts of seeds from 22 species of plants of the family meliaceae from a number of countries were prepared. the extracts were submitted to antifeedant and toxicity bioassays utilizing fall armyworm [spodoptera frugiperda (j.e. smith)] (lepidoptera: noctuidae) larvae and striped cucumber beetle [acalymma vittatum (f.)] (coleoptera: chrysomelidae) adults. toxicity tests were also performed with brine shrimp,anemia salina leach. feeding inhibition and mortality produced by some ... | 1987 | 24301362 |
| five new cytotoxic metabolites from the marine fungus neosartorya pseudofischeri. | the marine fungus neosartorya pseudofischeri was isolated from acanthaster planci from the south china sea. in a preliminary bioactivity screening, the crude methanol extract of the fungal mycelia showed significant inhibitory activity against the sf9 cell line from the fall armyworm spodoptera frugiperda. five novel compounds, including 5-olefin phenylpyropene a (1), 13-dehydroxylpyripyropene a (4), deacetylsesquiterpene (7), 5-formyl-6-hydroxy-8-isopropyl-2- naphthoic acid (9) and 6,8-dihydrox ... | 2016 | 26771621 |
| suitability of creeping bentgrass and bermudagrass cultivars for black cutworms and fall armyworms (lepidoptera: noctuidae). | the black cutworm, agrotis ipsilon hufnagel, and fall armyworm, spodoptera frugiperda smith (lepidoptera: noctuidae), are common turfgrass pests of golf courses in the southeastern united states. heat-tolerant bentgrass (agrostis stolonifera l.) cultivars are expanding the range of bentgrass further south, but these cultivars have not been studied for their potential host plant resistance to black cutworm or fall armyworm. the goals of the study were to investigate feeding response of black cutw ... | 2015 | 26470340 |
| pest protection conferred by a beta vulgaris serine proteinase inhibitor gene. | proteinase inhibitors provide a means of engineering plant resistance to insect pests. a beta vulgaris serine proteinase inhibitor gene (bvsti) was fused to the constitutive camv35s promoter for over-expression in nicotiana benthamiana plants to study its effect on lepidopteran insect pests. independently derived bvsti transgenic tobacco t2 homozygous progeny were shown to have relatively high bvsti gene transcript levels. bvsti-specific polyclonal antibodies cross-reacted with the expected 30 k ... | 2013 | 23468963 |
| multispecies trapping ofhelicoverpa (heliothis) zea, spodoptera frugiperda, pseudaletia unipuncta, andagrotis ipsilon (lepidoptera: noctuidae). | multispecies sex pheromone trapping (trapping of more than one species in the same trap) for the bollworm,helicoverpa (heliothis) zea (boddie); fall armyworm,spodoptera frugiperda (j.e. smith); armyworm,pseudaletia unipuncta (haworth); and the black cutworm,agrotis ipsilon (hufnagel) was evaluated. baiting of individual traps with all possible combinations of the four species taken two at a time caused a reduction in the catches of at least one of any two species involved when compared to traps ... | 1990 | 24263443 |
| acute and chronic toxicities of an annonin-based commercial bioinsecticide and a joint mixture with a limonoid-based formulation to the fall armyworm. | the bioactivity of a newly registered annonin-based formulation (anosom® 1 ec) was investigated for use against the fall armyworm spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae), both singly and mixed with a limonoid-based formulation (azamax® 1.2 ec) using dietary exposure bioassays. anosom® 1 ec caused significant mortality in s. frugiperda larvae exposed to treated artificial media, with the activity level dependent on both the concentration and the exposure time. in addition to e ... | 2017 | 27730568 |
| synergy between rhinacanthins from rhinacanthus nasutus in inhibition against mosquito cytochrome p450 enzymes. | the cytochrome p450 monooxygenases play a major role in insecticide detoxification and become a target for development of insecticide synergists. in this study, a collection of rhinacanthins (rhinacanthin-d, -e, -g, -n, -q, and -h/i) purified from rhinacanthus nasutus, in addition to previously purified rhinacanthin-b and -c, were isolated. these compounds displayed various degrees of inhibition against benzyloxyresorufin-o-debenzylation mediated by cyp6aa3 and cyp6p7 which were implicated in py ... | 2015 | 25869958 |
| structure–function relationships of inhibition of mosquito cytochrome p450 enzymes by flavonoids of andrographis paniculata. | the cytochrome p450 monooxygenases are known to play a major role in pyrethroid resistance, by means of increased rate of insecticide detoxification as a result of their overexpression. inhibition of detoxification enzymes may help disrupting insect detoxifying defense system. the anopheles minimus cyp6aa3 and cyp6p7 have shown pyrethroid degradation activity and been implicated in pyrethroid resistance. in this study inhibition of the extracts and constituents of andrographis paniculata nees. l ... | 2014 | 25015047 |
| cell culture for production of insecticidal viruses. | while large-scale culture of insect cells will need to be conducted using bioreactors up to 10,000 l scale, many of the main challenges for cell culture-based production of insecticidal viruses can be studied using small-scale (20-500 ml) shaker/spinner flasks, either in free suspension or using microcarrier-based systems. these challenges still relate to the development of appropriate cell lines, stability of virus strains in culture, enhancing virus yields per cell, and the development of seru ... | 2016 | 27565495 |
| pyramids of qtls enhance host-plant resistance and bt-mediated resistance to leaf-chewing insects in soybean. | qtl-m and qtl-e enhance soybean resistance to insects. pyramiding these qtls with cry1ac increases protection against bt-tolerant pests, presenting an opportunity to effectively deploy bt with host-plant resistance genes. plant resistance to leaf-chewing insects minimizes the need for insecticide applications, reducing crop production costs and pesticide concerns. in soybean [glycine max (l.) merr.], resistance to a broad range of leaf-chewing insects is found in pi 229358 and pi 227687. pi 2293 ... | 2016 | 26724806 |
| factors influencing expression of antixenosis in soybean to anticarsia gemmatalis and spodoptera frugiperda (lepidoptera: noctuidae). | this study aimed to evaluate some factors that influence the expression of antixenosis in soybean genotypes against anticarsia gemmatalis hübner and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae). free-choice and no-choice feeding assays were performed with the resistant soybean genotype iac 100 and the susceptible genotype brsgo 8360 using a. gemmatalis and s. frugiperda larvae. the following factors that may affect expression of resistance were evaluated: one larva versus two lar ... | 2015 | 26470136 |
| extensive transcription analysis of the hyposoter didymator ichnovirus genome in permissive and non-permissive lepidopteran host species. | ichnoviruses are large dsdna viruses that belong to the polydnaviridae family. they are specifically associated with endoparasitic wasps of the family ichneumonidae and essential for host parasitization by these wasps. we sequenced the hyposoter didymator ichnovirus (hdiv) encapsidated genome for further analysis of the transcription pattern of the entire set of hdiv genes following the parasitization of four different lepidopteran host species. the hdiv genome was found to consist of at least 5 ... | 2014 | 25117496 |
| improved replication of the baculovirus anticarsia gemmatalis nucleopolyhedrovirus (agmnpv) in vitro using proteins from lonomia obliqua hemolymph. | the baculovirus anticarsia gemmatalis nucleopolyhedrovirus (agmnpv), a member of the family baculoviridae, has been widely applied as a biopesticide for the control of the velvetbean caterpillar, a pest of soybean crop field. baculoviruses are considered safe and efficient agents for this purpose, because they do not infect vertebrates, being safe for the health of humans and animals, as well as to the environment. the objective of this work was to identify proteins obtained from lonomia obliqua ... | 2015 | 24969017 |
| effects of rag1 on the preference and performance of soybean defoliators. | the rag1 gene confers antibiotic resistance to soybean aphid, aphis glycines matsumura (hemiptera: aphididae), and in 2010, varieties expressing rag1 were released for commercial use in the united states. we do not know how rag1 varieties will influence the broader community of defoliating insects that inhabit soybean fields. in 2010 and 2011, the preference and performance of pest insects that defoliate soybeans [glycines max (l.) merr] were tested using rag1 and aphid-susceptible varieties. th ... | 2013 | 24498760 |
| xanthine toxicity to caterpillars synergized by allopurinol, a xanthine dehydrogenase/oxidase inhibitor. | xanthine (2,6-dioxypurine), which occurs in certain legumes and other plants, was fed in artificial diet to larvae of two noctuid moth species, a legume specialist,anticarsia gemmatalis, and a generalist,spodoptera frugiperda. in addition, diets either lacked or contained allopurinol (4-hydroxypyrazolo(3,4-d)-pyrimidine), an inhibitor of xanthine dehydrogenase and oxidase, enzymes that convert xanthine to uric acid. xanthine alone (up to 2% fresh mass, fm) had little deleterious effect on either ... | 1993 | 24248717 |
| influence of pheromone trap color and design on capture of male velvetbean caterpillar and fall armyworm moths (lepidoptera: noctuidae). | three pheromone traps were evaluated in paired field trials for effectiveness in capturing wild male velvetbean caterpillar moths (vbc),anticarsia gemmatalis hübner, and fall armyworm moths (faw),spodoptera frugiperda (j.e. smith), using a wind-oriented trapping device. the traps were: (1) the standard multicolored bucket trap consisting of a forest green canopy, yellow funnel, white bucket and open (i.e., single wire) pheromone holder; (2) a forest green monocolored bucket trap; and (3) the mul ... | 1989 | 24272181 |
| detoxification of isothiocyanate allelochemicals by glutathione transferase in three lepidopterous species. | glutathione transferase activity towards various plant isothiocyanates was studied in larvae of the two generalists, fall armyworm [spodoptera frugiperda (j.e. smith)], and cabbage looper [trichoplusia ni (hübner)], and the specialist, velvetbean caterpillar (anticarsia gemmatalis hübner) using the midgut soluble fraction as enzyme source. the generalists, but not the specialist, are adapted to feeding on isothiocyanate-containing crucifers. allyl and benzyl isothiocyanate were found to be metab ... | 1988 | 24276210 |
| microsomal oxidation of allelochemicals in generalist (spodoptera frugiperda) and semispecialist (anticarsia gemmatalis) insect. | midgut microsomes prepared from larvae of the fall armyworm [spodoptera frugiperda (j.e smith)], a generalist insect, and the velvetbean caterpillar (anticarsia gemmatalis hubner), a semispecialist, were used to study their oxidative activity toward a variety of allelochemicals. allelochemicals such as terpenoids, alkaloids, indoles, glucosinolates, flavonoids, coumarins, cardenolides, phenylpropenes, and a ketohydrocarbon were all metabolized by the microsomal cytochrome p-450 monooxygenases in ... | 1987 | 24301885 |
| development of an enzyme-linked-receptor assay based on syrian hamster β2-adrenergic receptor for detection of β-agonists. | β-adrenergic agonists (β-agonists) are illegally used in animal husbandry, threatening the health of consumers. to realize multianalyte detection of β-agonists, a β2-adrenergic receptor (β2-ar) was cloned from syrian hamster lung and heterogeneously expressed by spodoptera frugiperda (sf9) cells. the recombinant β2-ar was purified from intracellular soluble proteins of infected sf9 cells, and was utilized to establish an enzyme-linked-receptor assay (elra) to detect a group of β-agonists simulta ... | 2014 | 24853343 |
| a comprehensive study on apoptosis induction by azadirachtin in spodoptera frugiperda cultured cell line sf9. | the induction of apoptosis by azadirachtin, a well-known botanical tetranortriterpenoid isolated from the neem tree (azadirachta indica a. juss) and other members of the meliaceae, was investigated in spodoptera frugiperda cultured cell line (sf9). morphological changes in sf9 cells treated by various concentrations of azadirachtin were observed at different times under light microscopy. morphological and biochemical analysis indicated that sf9 cells treated by 1.5 μg/ml azadirachtin showed typi ... | 2015 | 25828604 |
| effect of meliaceous seed extracts on growth and survival ofspodoptera frugiperda (j.e. smith). | hexane and ethanol extracts of seeds from 10 plant species (including neem-azadirachta indica a. juss.) of the family meliaceae were incorporated into artificial diet at various doses and fed to fall armyworm [spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae)] larvae in nochoice tests. all produced significant mortality, reduced larval growth rate, increased time to pupation, or all three, at some concentration. the two highest doses of all of the ethanol extracts caused 100% mortalit ... | 1989 | 24271431 |
| herbivore damage and prior egg deposition on host plants influence the oviposition of the generalist moth trichoplusia ni (lepidoptera: noctuidae). | female insects have the difficult task of locating host plants that maximize the survival and success of their offspring. in this study, the oviposition preferences of the cabbage looper moth, trichoplusia ni (hübner), for soybean plants, glycine max (l.), under various treatments-undamaged, mechanically damaged, damaged by t. ni or spodoptera frugiperda (smith) larvae or by bemisia tabaci (gennadius) adults, egg-free plants, and plants previously oviposited by conspecific or heterospecific fema ... | 2016 | 27591288 |
| potato tuber herbivory increases resistance to aboveground lepidopteran herbivores. | plants mediate interactions between aboveground and belowground herbivores. although effects of root herbivory on foliar herbivores have been documented in several plant species, interactions between tuber-feeding herbivores and foliar herbivores are rarely investigated. we report that localized tuber damage by tecia solanivora (guatemalan tuber moth) larvae reduced aboveground spodoptera exigua (beet armyworm) and spodoptera frugiperda (fall armyworm) performance on solanum tuberosum (potato). ... | 2016 | 27147449 |
| an elicitor in caterpillar oral secretions that induces corn seedlings to emit chemical signals attractive to parasitic wasps. | regurgitate of corn-fed beet armyworm (baw) caterpillars,spodoptera exigua, when applied to damaged sites of corn (zea mays) seedlings, causes the release of relatively large amounts of terpenes by the seedlings several hours later. this plant response could be induced by merely placing the cut stem of seedlings in a solution of baw regurgitate for 12 hr, a response that could not be induced by placing seedlings in water only. regurgitate of baw fed various diets, including a minimal diet of fil ... | 1993 | 24248945 |
| genetic basis of allochronic differentiation in the fall armyworm. | very little is known on how changes in circadian rhythms evolve. the noctuid moth spodoptera frugiperda (lepidoptera: noctuidae) consists of two strains that exhibit allochronic differentiation in their mating time, which acts as a premating isolation barrier between the strains. we investigated the genetic basis of the strain-specific timing differences to identify the molecular mechanisms of differentiation in circadian rhythms. | 2017 | 28264650 |
| a characterization of structural proteins expressed by bombyx mori bidensovirus. | bombyx mori bidensiovirus (bmbdv) is a species of bidensovirus that has been was placed into a new genus within the new family bidnaviridae by the international committee on taxonomy of viruses. bmbdv causes fatal flacherie disease in silkworms, which causes large losses to the sericulture industry. bmbdv contains two sets of complementary linear single-stranded dnas of approximately 6.5kb (viral dna 1, vd1) and 6.0kb (viral dna 2, vd2). vd1 and vd2 are encapsidated in separate icosahedral non-e ... | 2017 | 28057460 |
| production of human type ii collagen using an efficient baculovirus-silkworm multigene expression system. | human type ii collagen is a macromolecular protein found throughout the human body. the baculovirus expression vector system is one of the most ideal systems for the routine production and display of recombinant eukaryotic proteins in insect, larvae, and mammalian cells. we use this system to express a full-length gene, human type ii collagen cdna (4257 bp), in cultured spodoptera frugiperda 9 cells (sf9), bombyx mori cells, and silkworm larvae. in this study, the expression of human type ii col ... | 2016 | 27669694 |
| a new cell line derived from embryonic tissues of holotrichia parallela (coleoptera:scarabaeidae). | holotrichia parallela is an important agricultural underground insect pest and also an edible and medicinal insect. establishing a new cell line of h. parallela will provide a rapid and convenient tool for the studies on its physiology, pathology, and gene functions. in this study, by using the embryonic tissue of h. parallela as the material, we established a new cell line named hp-e-1. the microscopic observation of its morphological characteristics revealed that its cellular morphology was ma ... | 2016 | 27083164 |
| insect larvae: a new platform to produce commercial recombinant proteins. | in biotechnology, the expression of recombinant proteins is a constantly growing field and different hosts are used for this purpose. some valuable proteins cannot be produced using traditional systems. insects from the order lepidoptera infected with recombinant baculovirus have appeared as a good choice to express high levels of proteins, especially those with post-translational modifications. lepidopteran insects, which are extensively distributed in the world, can be used as small protein fa ... | 2016 | 26956108 |
| insect cell transformation vectors that support high level expression and promoter assessment in insect cell culture. | a somatic transformation vector, pdp9, was constructed that provides a simplified means of producing permanently transformed cultured insect cells that support high levels of protein expression of foreign genes. the pdp9 plasmid vector incorporates dna sequences from the junonia coenia densovirus that are involved in integration of the densovirus in insect cell chromosomes and a promoter/enhancer system that results in high levels of expression. the plasmid also contains two markers that permit ... | 2016 | 26794473 |
| comparative transcriptome analysis of lufenuron-resistant and susceptible strains of spodoptera frugiperda (lepidoptera: noctuidae). | the evolution of insecticide resistance in spodoptera frugiperda (lepidoptera: noctuidae) has resulted in large economic losses and disturbances to the environment and agroecosystems. resistance to lufenuron, a chitin biosynthesis inhibitor insecticide, was recently documented in brazilian populations of s. frugiperda. thus, we utilized large-scale cdna sequencing (rna-seq analysis) to compare the pattern of gene expression between lufenuron-resistant (luf-r) and susceptible (luf-s) s. larvae in ... | 2015 | 26589731 |
| genome wide screening of rnai factors of sf21 cells reveal several novel pathway associated proteins. | rna interference (rnai) leads to sequence specific knock-down of gene expression and has emerged as an important tool to analyse gene functions, pathway analysis and gene therapy. although rnai is a conserved cellular process involving common elements and factors, species-specific differences have been observed among different eukaryotes. identification of components for rnai pathway is pursued intensively and successful genome-wide screens have been performed for components of rnai pathways in ... | 2014 | 25199785 |
| a draft genome assembly of the army worm, spodoptera frugiperda. | spodoptera is an agriculturally important pest insect and studies in understanding its biology have been limited by the unavailability of its genome. in the present study, the genomic dna was sequenced and assembled into 37,243 scaffolds of size, 358 mb with n50 of 53.7 kb. based on degree of identity, we could anchor 305 mb of the genome onto all the 28 chromosomes of bombyx mori. repeat elements were identified, which accounts for 20.28% of the total genome. further, we predicted 11,595 genes, ... | 2014 | 24984256 |
| cloning and functional characterization of the lymantria dispar initiator caspase dronc. | ld652y cells from the gypsy moth, lymantria dispar, are extremely sensitive to various apoptotic stimuli, whereas bm-n cells from the silkworm, bombyx mori, are relatively resistant to apoptotic stimuli. we previously cloned and characterized a b. mori homologue (bm-dronc) of drosophila melanogaster dronc. in the present study, we cloned and characterized an l. dispar homologue of dronc (ld-dronc) comparatively with bm-dronc. the open reading frame of ld-dronc consisted of 1329bp that was predic ... | 2013 | 23743202 |
| micrornaome of spodoptera frugiperda cells (sf9) and its alteration following baculovirus infection. | micrornas (mirnas) as small non-coding rnas play important roles in many biological processes such as development, cell signalling and immune response. studies also suggest that mirnas are important in host-virus interactions where the host limits virus infection by differentially expressing mirnas that target essential viral genes. here, we identified conserved and new mirnas from spodoptera frugiperda cells (sf9) using a combination of deep sequencing and bioinformatics as well as experimental ... | 2013 | 23407421 |
| molecular cloning and characterization of the α-glucosidase ii from bombyx mori and spodoptera frugiperda. | the α-glucosidase ii (gii) is a heterodimer of α- and β-subunits and important for n-glycosylation processing and quality control of nascent glycoproteins. although high concentration of α-glucosidase inhibitors from mulberry leaves accumulate in silkworms (bombyx mori) by feeding, silkworm does not show any toxic symptom against these inhibitors and n-glycosylation of recombinant proteins is not affected. we, therefore, hypothesized that silkworm gii is not sensitive to the α-glucosidase inhibi ... | 2013 | 23376632 |
| evolutionary history of x-tox genes in three lepidopteran species: origin, evolution of primary and secondary structure and alternative splicing, generating a repertoire of immune-related proteins. | the proteins of the x-tox family have imperfectly conserved tandem repeats of several defensin-like motifs known as cysteine-stabilized αβ (cs-αβ) motifs. these immune-related proteins are inducible and expressed principally in hemocytes, but they have lost the antimicrobial properties of the ancestral defensins from which they evolved. we compared x-tox gene structure and expression in three lepidopteran species (spodoptera frugiperda, helicoverpa armigera and bombyx mori). synteny and phylogen ... | 2013 | 23142192 |
| a baculovirus isolated from wild silkworm encompasses the host ranges of bombyx mori nucleopolyhedrosis virus and autographa californica multiple nucleopolyhedrovirus in cultured cells. | a baculovirus, named bomanpv s2, was isolated from a diseased larva of the wild silkworm, bombyx mandarina. notably, bomanpv s2 exhibited a distinguishing feature in that its host range covered that of both bombyx mori nucleopolyhedrosis virus (bmnpv) and autographa californica multiple nucleopolyhedrovirus (acmnpv) in cultured cells. it could replicate in cells of b. mori (bm5 and bmn), spodoptera frugiperda (sf9) and trichoplusia ni (tn-5b1-4). however, occlusion-derived virions of bomanpv s2 ... | 2012 | 22855783 |
| acetylcholinesterase inhibitory activity of haplophyton cimicidum. | in oaxaca, méxico, haplophyton cimicidum is used in combination with other plants as part of a folk strategy against arthropod pests of crops and cattle. methanolic and crude alkaloid (acid-base extraction) extracts of h. cimicidum leaves were analysed by thin-layer chromatography (tlc) and assayed in vitro for acetylcholinesterase (ache) inhibitory activity using spodoptera frugiperda homogenates as source of enzyme. derivatised chromatographic plates indicated the presence of indoles and alkal ... | 2014 | 24484055 |
| modification of p115rhogef ser(330) regulates its rhogef activity. | p115rhogef is a member of a family of rho-specific guanine nucleotide exchange factors that also contains a regulator of g protein signaling homology domain (rh-rhogefs) that serves as a link between gα13 signaling and rhoa activation. while the mechanism of regulation of p115rhogef by gα13 is becoming well-known, the role of other regulatory mechanisms, such as post-translational modification or autoinhibition, in mediating p115rhogef activity is less well-characterized. here, putative phosphor ... | 2013 | 23816534 |
| redescription of campoletis sonorensis (cameron, 1886) (hymenoptera, ichneumonidae, campopleginae), parasitoid of spodoptera frugiperda (j. e. smith, 1797) (lepidoptera, noctuidae) in brazil. | the fall armyworm spodoptera frugiperda (lepidoptera; noctuidae) is a voracious pest of numerous crops of economic importance throughout the new world. in brazil, its larvae are attacked by several species of parasitoid wasps, making them potential candidate as biological control agents against this pest. a survey of the parasitoid fauna on s. frugiperda in maize crops throughout brazil reveals two species of campoletis, which are morphologicaly very similar species. in this paper we combine the ... | 2015 | 26675917 |
| redescription of campoletis sonorensis (cameron, 1886) (hymenoptera, ichneumonidae, campopleginae), parasitoid of spodoptera frugiperda (j. e. smith, 1797) (lepidoptera, noctuidae) in brazil. | abstractthe fall armyworm spodoptera frugiperda (lepidoptera; noctuidae) is a voracious pest of numerous crops of economic importance throughout the new world. in brazil, its larvae are attacked by several species of parasitoid wasps, making them potential candidate as biological control agents against this pest. a survey of the parasitoid fauna on s. frugiperda in maize crops throughout brazil reveals two species of campoletis, which are morphologicaly very similar species. in this paper we com ... | 2015 | 26560660 |