| effect of lysimachia ramosa (primulaceae) on helminth parasites: motility, mortality and scanning electron microscopic observations on surface topography. | the alcoholic extract of lysimachia ramosa wall (primulaceae) was tested in vitro against helminth parasites, fasciolopsis buski and ascaris suum, from porcine hosts and raillietina echinobothrida from domestic fowl. the live adult parasites, collected from a freshly autopsied host, were exposed to different concentrations (5-50mg) of the test plant extract in physiological phosphate-buffered saline (pbs) having 0.1% dimethyl sulphoxide (dmso) at 37+/-1 degrees c. the treated parasites revealed ... | 2010 | 20138704 |
| characterization of a caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from haemonchus contortus (nematoda). | a full-length complementary dna (cdna; designated hc-stp-1) encoding a serine/threonine phosphatase (hc-stp-1) was isolated from haemonchus contortus, a strongylid nematode parasite of small ruminants. hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in females, early larval stages or eggs. the full-length gene (2854 bp) contained ten exons and nine introns, and encoded a cdna of 951 bp. comparisons of the conceptually translated protein (316 amino ac ... | 2010 | 20153820 |
| the increase in seroprevalence to toxocara canis in asthmatic children is related to cross-reaction with ascaris suum antigens. | it has been suggested that the presence of toxocara canis larvae in lungs is an underlying factor in the onset of asthma. although the association of asthma and seropositivity to toxocara has been observed, there are no studies that indicate whether these antibodies are specific against t. canis antigens. | 2010 | 20227158 |
| fate and transport of zoonotic, bacterial, viral, and parasitic pathogens during swine manure treatment, storage, and land application. | members of the public are always somewhat aware of foodborne and other zoonotic pathogens; however, recent illnesses traced to produce and the emergence of pandemic h1n1 influenza virus have increased the scrutiny on all areas of food production. the council for agricultural science and technology has recently published a comprehensive review of the fate and transport of zoonotic pathogens that can be associated with swine manure. the majority of microbes in swine manure are not zoonotic, but se ... | 2010 | 20348375 |
| molecular evidence for sustained transmission of zoonotic ascaris suum among zoo chimpanzees (pan troglodytes). | chimpanzees in the copenhagen zoo frequently excrete ascarid worms onto the cage floor in spite of a regular anthelmintic treatment program. previously it had been shown that the source of the infections was of pig origin. however, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the zoo. we found that isolated eggs were able to embryonate to the infective j3 stage and pcr-rflp analysis on ... | 2010 | 20400228 |
| serological, clinical and epidemiological evaluation of toxocariasis in urban areas of south brazil. | toxocariasis is a worldwide public-health problem that poses major risks to children who may accidentally ingest embryonated eggs of toxocara. the objectives of this study were to investigate the occurrence of anti-toxocara spp. antibodies in children and adolescents and the variables that may be involved, as well as environmental contamination by toxocara spp. eggs, in urban recreation areas of north central mesoregion, paraná state, brazil. from june 2005 to march 2007. a total of 376 blood sa ... | 2010 | 20464126 |
| il-13 is a novel therapeutic target in allergic asthma. | evaluation of: bree a, schlerman fj, wadanoli m et al. il-13 blockade reduces lung inflammation after ascaris suum challenge in cynomolgus monkeys. j. allergy clin. immunol. 119, 1251-1257 (2007). there is abundant evidence supporting il-13 as having a central role in the pathology of allergic asthma. il-13 induces immunological responses relevant to allergic asthma and exerts effects on airway structural cells, leading to remodeling and enhanced contractility of airway smooth muscle. this paper ... | 2007 | 20477017 |
| dynamical insight into caenorhabditis elegans eif4e recognition specificity for mono-and trimethylated structures of mrna 5' cap. | specific recognition and binding of the ribonucleic acid 5' termini (mrna 5' cap) by the eukaryotic translation initiation factor 4e (eif4e) is a key, rate limiting step in translation initiation. contrary to mammalian and yeast eif4es that discriminate in favor of 7-methylguanosine cap, three out of five eif4e isoforms from the nematode caenorhabditis elegans as well as eif4es from the parasites schistosome mansoni and ascaris suum, exhibit dual binding specificity for both 7-methylguanosine-an ... | 2011 | 20535623 |
| effect of ascaris suum infection on performance of fattening pigs. | scientific investigations of production losses in pigs due to roundworms are rarely conducted in commercial farms, despite the fact that this information is needed to decide whether anthelmintic treatment is cost-effective or not. therefore, the aim of our study was to compare performance in fattening pigs treated or not treated for ascaris suum infections. two danish pig farms producing fatteners and showing a. suum-induced liver white spot prevalences of 10-33% were selected for the study. in ... | 2010 | 20541323 |
| matrix metalloproteinase-12 is a therapeutic target for asthma in children and young adults. | matrix metalloproteinase (mmp)-12-mediated pathologic degradation of the extracellular matrix and the subsequent repair cycles influence the airway changes in patients with asthma and chronic obstructive pulmonary disease (copd). the common serine variant at codon 357 of the mmp12 gene (rs652438) is associated with clinical manifestations consistent with more aggressive matrix degradation in other tissues. | 2010 | 20546881 |
| molecular differentiation and phylogenetic relationships of three angiostrongylus species and angiostrongylus cantonensis geographical isolates based on a 66-kda protein gene of a. cantonensis (nematoda: angiostrongylidae). | the phylogenetic relationships and molecular differentiation of three species of angiostrongylid nematodes (angiostrongylus cantonensis, angiostrongylus costaricensis and angiostrongylus malaysiensis) were studied using the ac primers for a 66-kda protein gene of a. cantonensis. the ac primers successfully amplified the genomic dna of these angiostrongylid nematodes. no amplification was detected for the dna of ascaris lumbricoides, ascaris suum, anisakis simplex, gnathostoma spinigerum, toxocar ... | 2010 | 20566366 |
| production of monoclonal antibody against toxocara cati second-stage larvae and its application for the detection of circulating antigens. | toxocariasis is an important zoonotic disease caused by the second-stage larvae of toxocara species. human toxocariasis is usually diagnosed by serological methods. we prepared monoclonal antibody specific for toxocara cati (t. cati) and performed the following experiments using this antibody to increase the sensitivity of our capture elisa for antigen detection. the isotype of monoclonal antibody was igg3. using this monoclonal antibody, a capture elisa was developed. the produced monoclonal an ... | 2010 | 20568996 |
| identification of factors influencing the occurrence of milk spot livers in slaughtered pigs: a novel approach to understanding ascaris suum epidemiology in british farmed pigs. | ascariosis is the most important internal parasitism present worldwide in farmed pigs. milk spots are healing lesions occurring when ascaris suum larvae migrate through the liver. this study aimed to identify current husbandry practices (e.g. wet/compound feeding, outdoors/indoors production, bedded/slatted floors) that influence the prevalence of milk spots in batches of slaughtered pigs, accounting for geographical locations and seasonality. farm information was accessed through the british fa ... | 2010 | 20667659 |
| ascaris suum: rnai mediated silencing of enolase gene expression in infective larvae. | ascaris suum is an important parasite of pigs that causes tremendous economic losses globally to agriculture and animal husbandry annually. rna interference (rnai) technology has been described as a successful and useful approach for the elucidation of gene function in parasitic nematodes. in the present study, rnai was used to silence the expression of a gene encoding enolase in a. suum by soaking infective larvae in double-stranded rna derived from an est (representing as-enol-1) selected from ... | 2011 | 20691683 |
| kinetics of inactivation of indicator pathogens during thermophilic anaerobic digestion. | thermophilic anaerobic sludge digestion is a promising process to divert waste to beneficial use, but an important question is the required temperature and holding time to achieve a given degree of pathogen inactivation. in this study, the kinetics of inactivation of ascaris suum and vaccine strain poliovirus type 1 (pvs-1), selected as indicators for helminth ova and enteric viruses respectively, were determined during anaerobic digestion at temperatures ranging from 51 to 56 °c. inactivation o ... | 2010 | 20692678 |
| rnai-mediated silencing of a novel ascaris suum gene expression in infective larvae. | in the present study, the potential of rna interference (rnai) as a gene silencing tool and the resultant effects on ascaris suum larval development was examined by targeting a gene (represented by the est 06g09) specifically expressed in the infective larvae of a. suum. balb/c mice were infected with rnai-treated larvae. the results showed that the target gene was silenced after soaking for 72 h, and the survival rate of the rnai-treated larvae was reduced by 17.25% (p<0.01). a significant diff ... | 2010 | 20717832 |
| mapping neuropeptide expression by mass spectrometry in single dissected identified neurons from the dorsal ganglion of the nematode ascaris suum. | we have developed a method for dissecting single neurons from the nematode ascaris suum, in order to determine their peptide content by mass spectrometry (ms). in this paper, we use maldi-tof ms and tandem ms to enumerate and sequence the peptides present in the two neurons, ala and rid, that comprise the dorsal ganglion. we compare the peptide content determined by ms with the results of immunocytochemistry and in situ hybridization of previously isolated peptides af2, af8 and 6 peptides encode ... | 2010 | 20806053 |
| molecular cloning of an immunogenic protein of baylisascaris procyonis and expression in escherichia coli for use in developing improved serodiagnostic assays. | larva migrans caused by baylisascaris procyonis is an important zoonotic disease. current serological diagnostic assays for this disease depend on the use of the parasite's larval excretory-secretory (es) antigens. in order to identify genes encoding es antigens and to generate recombinant antigens for use in diagnostic assays, construction and immunoscreening of a b. procyonis third-stage larva cdna expression library was performed and resulted in identification of a partial-length cdna clone e ... | 2010 | 20926699 |
| ascaris and ascariasis. | ascaris lumbricoides and ascaris suum are widespread parasitic nematodes of humans and pigs respectively. recent prevalence data suggests that approximately 1.2 billion people are infected. adult worms exhibit an overdispersed frequency distribution in their hosts and individuals harbouring heavy burdens display associated morbidity. in this review, we describe the parasite, its distribution and measures undertaken to control infection. | 2010 | 20934531 |
| molecular genetic evidence for polyandry in ascaris suum. | the aim of this study was to determine whether single ascaris suum female could mate with multiple males. seven sex-linked microsatellite markers were employed and paternal genetic analyses were conducted. totally, 62 offspring individuals from three single females were screened, and the numbers of fathers in each family were determined using allele counting methods and the program gerud, version 2.0. the seven sex-linked microsatellite loci showed high polymorphism and revealed that one out of ... | 2010 | 20949281 |
| pas-1, an ascaris suum protein, modulates allergic airway inflammation via cd8+γδtcr+ and cd4+cd25+foxp3+ t cells. | we have previously demonstrated that pas-1, a 200 kda protein from ascaris suum, has a potent immunomodulatory effect on humoral and cell-mediated responses induced by apas-3 (an allergenic protein from a. suum) or unrelated antigens. in this study, we investigated the mechanisms by which pas-1 is able to induce this effect on an allergic airway inflammation induced by ova in mice. c57bl/6 mice were adoptively transferred on day 0 with seven different pas-1-primed cell populations: pas-1-primed ... | 2010 | 21044123 |
| inactivation of ascaris suum by short-chain fatty acids. | ascaris suum eggs were inactivated in distilled water and digested sludge by butanoic, pentanoic, and hexanoic acids. the fatty acids (short-chain fatty acids [scfa]) were effective only when protonated and at sufficient concentrations. the conjugate bases were not effective at the concentrations evaluated. predictions from an inhibition model (50% inhibitory concentration [ic(50)]) based on quantitative structure-activity relationships were congruent with inactivation data. | 2010 | 21057018 |
| ovicidal activity of seven pochonia chlamydosporia fungal isolates on ascaris suum eggs. | the ovicidal effect of the nematophagous fungus pochonia chlamydosporia on eggs of ascaris suum was tested under laboratory conditions. a. suum eggs were plated on 2% water-agar with seven fungal isolates (isol. 5, isol. 31, isol. 1, vc1, isol. 12, isol. 22 and vc4) and control without fungus. after 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect with ... | 2010 | 21088892 |
| prevalence of gastrointestinal nematodes in growing pigs in kabale district in uganda. | during the last 30 years, pig production in uganda and neighbouring counties has increased markedly. pigs are mainly kept as a source of income for small-scale farmers; however, the pig production is subject to several constraints, one of them being worm infections. a study was carried out in rural communities in kabale district in the south western part of uganda in september and october 2007 in order to estimate the prevalence of gastrointestinal nematode parasites in pigs based on coprologica ... | 2010 | 21088893 |
| ascariasis in japan: is pig-derived ascaris infecting humans? | human ascariasis is caused by infection with the common roundworm ascaris lumbricoides, although the pig roundworm ascaris suum has also been reported to infect humans and develop into the adult stage. to elucidate whether pig-derived ascaris infects humans in japan, 9 ascaris isolates obtained from japanese patients and a further 9 ascaris isolates of pig origin were analyzed to determine their internal transcribed spacer-1 sequences. six of the 9 clinical isolates showed the ascaris genotype w ... | 2010 | 21099099 |
| involvement of k(+) channels in the augmented nasal venous responsiveness to nitric oxide in rat model of allergic rhinitis. | one of the factors of nasal obstruction observed in allergic rhinitis is thought to be a dilatation of microveins in nasal mucosa, although the exact mechanism(s) is not fully understood. in nasal mucosae of repeatedly antigen challenged rats, no-induced venodilatation itself is augmented. in the present study, the roles of k(+) channels in sodium nitroprusside (no donor; snp)-induced venodilatation of nasal mucosae in antigen-challenged rats were investigated. | 2010 | 21108952 |
| effects of high pressure processing on hatching of eggs of the zoonotic rat tapeworm hymenolepis diminuta. | high hydrostatic pressure processing (hpp) is an effective non-thermal treatment to remove pathogens from a variety of food and food products. it has been extensively examined using prokaryotic organisms but has had limited study on eukaryotic organisms. treatment using hpp has been shown to be effective in inactivating nematode larvae in food and ascaris suum eggs. nothing is known on the efficacy of hpp on tapeworm cysts or eggs. eggs of important zoonotic tapeworms including echinococcus and ... | 2010 | 21131130 |
| presidential address. sanitizing sewage sludge: the intersection of parasitology, civil engineering, and public health. | | 2010 | 21158605 |
| potential risk of zoonotic infections in recreational areas visited by sus scrofa and vulpes vulpes. case study--wolin island, poland. | the relation between intestinal parasite prevalence in wild boars and red foxes and the sanitary condition of the soil in recreational estates were determined. the analysis was made based on 36 samples of boar faeces and 22 samples of fox faeces, collected in their habitat as well as 60 samples of soil from two recreational areas. two methods were used for faecal samples--flotation and direct faecal smear; and flotation in nano3 for soil samples examination. zoonotic nematode eggs were recovered ... | 2010 | 21174955 |
| neuropeptide physiology in helminths. | parasitic worms come from two distinct, distant phyla, nematoda (roundworms) and platyhelminthes (flatworms). the nervous systems of worms from both phyla are replete with neuropeptides and there is ample physiological evidence that these neuropeptides control vital aspects of worm biology. in each phyla, the physiological evidence for critical roles for helminth neuropeptides is derived from both parasitic and free-living members. in the nematodes, the intestinal parasite ascaris suum and the f ... | 2010 | 21189675 |
| immunizing pigs with ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity. | to determine whether purified ascaris suum haemoglobin (ashb) is a suitable vaccine candidate for the control of ascaris infections, pigs were vaccinated with ashb in combination with quila adjuvant and challenged with a. suum eggs. the number of liver lesions and worms in the intestine was assessed on day 14, 28 and 56 post-infection (p.i.). no significant differences were found in the number of worms recovered between vaccinated and control pigs on any of these days. however, significantly mor ... | 2011 | 21204852 |
| intestinal parasite infections in pigs and beef cattle in rural areas of chungcheongnam-do, korea. | the present study was performed to investigate the infection status of intestinal parasites in pigs and beef cattle in rural areas of chungcheongnam-do, korea. from november 2009 to april 2010, a total of 241 fecal samples of pigs and beef cattle (136 and 105, respectively) were examined by direct smear and centrifugal sedimentation methods. the overall positive rates of intestinal parasites among pigs and beef cattle were 73.5% and 4.8%, respectively, and the double-infection rate was 10.3% in ... | 2010 | 21234241 |
| validation of ethnoveterinary medicinal treatments. | | 2011 | 21269773 |
| phylogeography of ascaris lumbricoides and a. suum from china. | in order to obtain further understanding of genetic structure and evolutionary relationship of ascaris from humans and pigs, phylogeography study on 12 populations from six endemic regions in china was conducted using mitochondrial dna markers (cytochrome c oxidase subunit 1 (cox1) and nad1) and the software programs of dnasp 5.0, arlequin 3.0, mega 4.0, and network 4.5.1.6. results showed that (a) genetic diversity of ascaris varied with hosts and locations, but no distinct geographical distrib ... | 2011 | 21301872 |
| does meatiness of pigs depend on the level of gastro-intestinal parasites infection? | the aim of the present paper was to determine an influence of the presence and a level of intestine parasites infection on the quality of pork carcass expressed by the content of meat in carcass (meatiness) in pigs. the experimental part of the study was conducted on pigs farm produced in a closed cycle. the population in the study included 120 fattening pigs maintained in two keeping systems: group i--60 individuals kept on slatted floor, and group ii--60 individuals kept on deep litter. all th ... | 2011 | 21334086 |
| the role of intelectin-2 in resistance to ascaris suum lung larval burdens in susceptible and resistant mouse strains. | the underlying mechanism of predisposition to ascaris infection is not yet understood but host genetics are thought to play a fundamental role. we investigated the association between the intelectin-2 gene and resistance in f2 mice derived from mouse strains known to be susceptible and resistant to infection. ascaris larvae were isolated from murine lungs and the number of copies of the intelectin-2 gene was determined in f2 mice. intelectin-2 gene copy number was not significantly linked to lar ... | 2011 | 21349217 |
| prevalence and risk factors associated with intestinal parasites in pigs in chongqing, china. | from 2007 to 2009, the prevalence of intestinal parasites was investigated in intensive and extensive pig farms in chongqing, china. a total of 2971 samples from both sexes and five age categories (breeding boars, breeding sows, fatteners, growers and weaners) were evaluated by standard methods for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites. of the 2971 pigs sampled, 362(12.18%) were infected with ascaris suum, 301(10.13%) with trichuris suis, 301(10.13%) with ... | 2011 | 21349561 |
| antigen challenge influences various transcription factors of rat bronchus: protein/dna array study. | although the transcription factors are required for expression of the proinflammatory cytokines and immune proteins which are involved in airway hyperresponsiveness and inflammation of bronchial asthma, the antigen-induced alterations of the transcription factors in bronchi have not yet been revealed. therefore, in order to profile the alteration pattern of the transcription factors after antigen challenge in bronchi, we used protein/dna arrays. rats were sensitized and repeatedly challenged wit ... | 2011 | 21354352 |
| effect of short-term oral and inhaled corticosteroids on airway inflammation and responsiveness in a feline acute asthma model. | the objective of this study was to investigate whether high-dose inhaled fluticasone propionate (fp), alone or in combination with salmeterol (sal), is as effective as oral prednisolone in reducing airway inflammation and obstruction in cats with experimentally-induced acute asthma. six cats sensitised to ascaris suum (as) were enrolled in a prospective controlled therapeutic trial and underwent four aerosol challenges, at 1-month intervals with as allergen. the allergen - stimulated animals rec ... | 2011 | 21354836 |
| toxocara and ascaris seropositivity among patients suspected of visceral and ocular larva migrans in the netherlands: trends from 1998 to 2009. | toxocara canis, toxocara cati and ascaris suum are roundworms of dogs, cats and pigs, respectively, that can also infect humans. these zoonotic helminths have a worldwide distribution and are also endemic in the netherlands. infection with toxocara sp. may result in visceral larva migrans (vlm) or ocular larva migrans (olm) caused by the migrating larvae. although a. suum has been reported to occasionally mature to the adult stage in humans, clinical cases of vlm suspected to be caused by a. suu ... | 2011 | 21365288 |
| lactobacillus rhamnosus hn001 attenuates allergy development in a pig model. | probiotics have been studied as immunomodulatory agents of allergy. several human probiotic trials tracking the development of eczema and other forms of allergy have yielded inconsistent results. a recent infant study demonstrated that pre and postnatal lactobacillus rhamnosus hn001 (hn001) supplementation decreased the prevalence of eczema and ige associated eczema. however, the influence of hn001 on the incidence of wheeze, asthma, and/or other allergic manifestations has yet to be reported. | 2011 | 21386995 |
| a specific antibody to neuropeptide af1 (knefirfamide) recognizes a small subset of neurons in ascaris suum: differences from caenorhabditis elegans. | a monoclonal antibody, af1-003, highly specific to the ascaris suum neuropeptide af1 (knefirfamide), was generated. this antibody binds strongly to af1 and extremely weakly to other peptides with c-terminal firfamide: af5 (sgkptfirfamide), af6 (firfamide), and af7 (agprfirfamide). it does not recognize 35 other af (a. suum fmrfamide-like) peptides at the highest concentration tested, nor does it recognize fmrfamide. when crude peptide extracts of a. suum are fractionated by two-step hplc, the on ... | 2011 | 21452223 |
| on the mode of action of emodepside: slow effects on membrane potential and voltage-activated currents in ascaris suum. | background and purpose nematode parasites infect humans and domestic animals. treatment and prophylaxis relies on anthelmintic drugs because there are no effective vaccines. emodepside, a cyclooctadepsipeptide, is a more recently introduced drug which has a novel mode of action involving a calcium-activated potassium slo-1 channel in c. elegans, sometimes mediated by a latrophilin (lat) receptor. emodepside breaks resistance to the classical anthelmintics. the purpose of our experiments was to e ... | 2011 | 21486286 |
| discovery of neuropeptides in the nematode ascaris suum by database mining and tandem mass spectrometry. | liquid chromatography coupled with tandem mass spectrometry (lc-ms/ms) was used to discover peptides in extracts of the large parasitic nematode ascaris suum. this required the assembly of a new database of known and predicted peptides. in addition to those already sequenced, peptides were either previously predicted to be processed from precursor proteins identified in an a. suum library of expressed sequence tags (ests) or newly predicted from a library of a. suum genome survey sequences (gsss ... | 2011 | 21524146 |
| solution structure of a repeated unit of the aba-1 nematode polyprotein allergen of ascaris reveals a novel fold and two discrete lipid-binding sites. | nematode polyprotein allergens (npas) are an unusual class of lipid-binding proteins found only in nematodes. they are synthesized as large, tandemly repetitive polyproteins that are post-translationally cleaved into multiple copies of small lipid binding proteins with virtually identical fatty acid and retinol (vitamin a)-binding characteristics. they are probably central to transport and distribution of small hydrophobic compounds between the tissues of nematodes, and may play key roles in nut ... | 2011 | 21526216 |
| neurobiology of plant parasitic nematodes. | the regulatory constraints imposed on use of chemical control agents in agriculture are rendering crops increasingly vulnerable to plant parasitic nematodes. thus, it is important that new control strategies which meet requirements for low toxicity to non-target species, vertebrates and the environment are pursued. this would be greatly facilitated by an improved understanding of the physiology and pharmacology of these nematodes, but to date, these microscopic species of the phylum nematoda hav ... | 2011 | 21538093 |
| effects of palmitoylethanolamide on the cutaneous allergic inflammatory response in ascaris hypersensitive beagle dogs. | palmitoylethanolamide (pea) is an endogenous lipid mediator with anti-inflammatory and anti-hyperalgesic properties. the main objective of the present study was to evaluate the effects of pea on the cutaneous allergic inflammatory reaction induced by different immunological and non-immunological stimuli in hypersensitive dogs. six spontaneously ascaris hypersensitive beagle dogs were challenged with intradermal injections of ascaris suum extract, substance p and anti-canine ige, before and after ... | 2011 | 21601500 |
| complete mitochondrial genomes of baylisascaris schroederi, baylisascaris ailuri and baylisascaris transfuga from giant panda, red panda and polar bear. | roundworms of the genus baylisascaris are the most common parasitic nematodes of the intestinal tracts of wild mammals, and most of them have significant impacts in veterinary and public health. mitochondrial (mt) genomes provide a foundation for studying epidemiology and ecology of these parasites and therefore may be used to assist in the control of baylisascariasis. here, we determined the complete sequences of mtdnas for baylisascaris schroederi, baylisascaris ailuri and baylisascaris transf ... | 2011 | 21621593 |
| high pressure processing treatment prevents embryonation of eggs of trichuris vulpis and ascaris suum and induces delay in development of eggs. | high hydrostatic pressure processing (hpp) is an effective non-thermal treatment used to inactivate pathogens from a variety of food and food products. it has been extensively examined using prokaryotic organisms and protozoan's but has had limited study on metazoans. treatment using hpp has been shown to be effective in inactivating nematode larvae in food and preventing embryonation of ascaris suum eggs. we conducted experiments using eggs of the canine whipworm trichuris vulpis collected from ... | 2011 | 21632180 |
| rnai effector diversity in nematodes. | while rna interference (rnai) has been deployed to facilitate gene function studies in diverse helminths, parasitic nematodes appear variably susceptible. to test if this is due to inter-species differences in rnai effector complements, we performed a primary sequence similarity survey for orthologs of 77 caenorhabditis elegans rnai pathway proteins in 13 nematode species for which genomic or transcriptomic datasets were available, with all outputs subjected to domain-structure verification. our ... | 2011 | 21666793 |
| fate of the pathogen indicators phage φx174 and ascaris suum eggs during the production of struvite fertilizer from source-separated urine. | human urine has the potential to be a sustainable, locally and continuously available source of nutrients for agriculture. phosphate can be efficiently recovered from human urine in the form of the mineral struvite (mgnh(4)po(4)·6h(2)o). however, struvite formation may be coupled with the precipitation of other constituents present in urine including pathogens, pharmaceuticals, and heavy metals. to determine if struvite fertilizer presents a microbiological health risk to producers and end users ... | 2011 | 21807394 |
| morphological changes of ascaris spp. eggs during their development outside the host. | abstract information on the infective stage of ascaris lumbricoides and the pathological damage caused by the parasite is widely available in the literature. however, information about early embryonic development of a. lumbricoides and its life cycle outside the host is limited. the purpose of this study was to describe the morphological changes within the developing embryo during incubation in vitro at 28 c, as well as to explore differences in eggs viability during incubation. ascaris suum egg ... | 2011 | 21801007 |
| biological control of ascaris suum eggs by pochonia chlamydosporia fungus. | ascaris suum is a gastrointestinal nematode parasite of swines. the aim of this study was to observe pochonia chlamydosporia fungus on biological control of a. suum eggs after fungus passage through swines gastrointestinal tract. eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate vc4; group 2, treated with the fungus isolate vc1 and group 3 did not receive fungus (control). in the treated groups, each animal received a 9 g single ... | 2011 | 21796329 |
| molecular and catalytic properties of an arginine kinase from the nematode ascaris suum. | we amplified the cdna coding for arginine kinase (ak) from the parasitic nematode ascaris suum, cloned it in pmal plasmid and expressed the enzyme as a fusion protein with the maltose-binding protein. the whole cdna was 1260 bp, encoding 400 amino acids, and the recombinant protein had a molecular mass of 45,341 da. ascaris suum recombinant ak showed significant activity and strong affinity ( k _{m}^{arg} = 0.126\hairsp mm) for the substrate l-arginine. it also exhibited high catalytic efficienc ... | 2011 | 21781373 |
| new insights into the mechanism of fertilization in nematodes. | fertilization results from the fusion of male and female gametes in all sexually reproducing organisms. much of nematode fertility work was focused on caenorhabditis elegans and ascaris suum. the c. elegans hermaphrodite produces a limited number of sperm initially and then commits to the exclusive production of oocytes. the postmeiotic differentiation called spermiogenesis converts sessile spermatids into motile spermatozoa. the motility of spermatozoa depends on dynamic assembly and disassembl ... | 2011 | 21749902 |
| the polyprotein allergens of nematodes (npas) - structure at last, but still mysterious. | we are engaged in structural and functional studies of several types of lipid binding protein that are only found in nematodes. amongst these are the nematode polyprotein allergens (npas) and we now report the solution structure of aba-1a (as-npa-a1), the most repeated unit within the npa array of ascaris suum, which is almost identical in amino acid sequence to that of ascaris lumbricoides. the protein forms a slightly flattened, compact, globular fold consisting of a long central helix that pa ... | 2011 | 21741973 |
| sedimentation of helminth eggs in water. | helminth parasite eggs in low quality water represent health risks when used for irrigation of crops. the settling velocities of helminth eggs (ascaris suum, trichuris suis, and oesophagostomum spp.) and wastewater particles were experimentally determined in tap water and in wastewater using owen tubes. the settling velocities of eggs in tap water was compared with theoretical settling velocities calculated by stoke's law using measurements of size and density of eggs as well as density and visc ... | 2011 | 21741063 |
| risk factors associated with occurrence of nematodes in free range pigs in busia district, kenya. | nematode infections are a serious constraint to pig production, especially where free range pig keeping is practiced. this study investigated the epidemiology of nematodes in free range pigs in busia district, kenya. three hundred and six pigs from 135 farms were sampled for faeces that were analysed for nematode eggs per gram (epg) of faeces using the mcmaster technique. the nematode eggs were also identified to genus and species based on morphology. a questionnaire on risk factors was also adm ... | 2011 | 21833678 |
| helminth parasites in pigs: new challenges in pig production and current research highlights. | helminths in pigs have generally received little attention from veterinary parasitologists, despite ascaris suum, trichuris suis, and oesophagostomum sp. being common worldwide. the present paper presents challenges and current research highlights connected with these parasites. in danish swine herds, new indoor production systems may favour helminth transmission and growing knowledge on pasture survival and infectivity of a. suum and t. suis eggs indicates that they may constitute a serious thr ... | 2011 | 21684689 |
| deep small rna sequencing from the nematode ascaris reveals conservation, functional diversification, and novel developmental profiles. | eukaryotic cells express several classes of small rnas that regulate gene expression and ensure genome maintenance. endogenous sirnas (endo-sirnas) and piwi-interacting rnas (pirnas) mainly control gene and transposon expression in the germline, while micrornas (mirnas) generally function in post-transcriptional gene silencing in both somatic and germline cells. to provide an evolutionary and developmental perspective on small rna pathways in nematodes, we identified and characterized known and ... | 2011 | 21685128 |
| eosinophilic pneumonia due to visceral larva migrans possibly caused by ascaris suum: a case report and review of recent literatures. | we report the case of a 62-year-old man who developed eosinophilic pneumonia due to visceral larva migrans (vlm) that was possibly caused by ascaris suum. the patient, a resident of the middle kyushu area who was found of eating raw porcine liver, complained of dry cough without dyspnea. the chest radiography showed a migration of infiltrative shadow. transbronchial lung biopsy of the right middle lobe revealed massive infiltration of eosinophils. the multi-dot enzyme-linked immunosorbent assay ... | 2011 | 21937827 |
| changes in cyclic nucleotides, locomotory behavior, and body length produced by novel endogenous neuropeptides in the parasitic nematode ascaris suum. | recent technical advances have rapidly advanced the discovery of novel peptides, as well as the transcripts that encode them, in the parasitic nematode ascaris suum. here we report that many of these novel peptides produce profound and varied effects on locomotory behavior and levels of cyclic nucleotides in a. suum. we investigated the effects of 31 endogenous neuropeptides encoded by transcripts afp-1, afp-2, afp-4, afp-6, afp-7, and afp-9-14 (afp: ascaris fmrfamide-like precursor protein) on ... | 2011 | 21854812 |
| distribution and characteristics of abfs, cecropins, nemapores, and lysozymes in nematodes. | several groups of antimicrobial effector molecules have been identified in nematodes, but most studies have been limited to caenorhabditis elegans and, to a lesser extent, ascaris suum. although these two species are not closely related, they are not representative of overall nematode diversity. this study utilized available sequence information to investigate whether four groups of antimicrobial effectors (defensin-like antibacterial factors [abfs], cecropins, saposin domain-containing proteins ... | 2011 | 21978453 |
| developmentally programmed, rna-directed genome rearrangement in tetrahymena. | developmentally programmed genome rearrangement has been observed in a variety of eukaryotes from vertebrates to worms to protists, and it provides an interesting exception to the general rule of the constancy of the genome. dna elimination in the ciliated protozoan tetrahymena is one of the most well-characterized programmed genome rearrangement events. dna elimination in the newly formed macronucleus of tetrahymena is epigenetically regulated by the dna sequence of the parental macronucleus. d ... | 2011 | 22103557 |
| A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum. | C-type lectins (CTLs) are a group of proteins which bind to carbohydrate epitopes in the presence of Ca(2+), which have been described in a wide range of species. In this study, a cDNA sequence coding a putative CTL has been identified from the cDNA library constructed from the pig round worm Ascaris suum lung L3 (LL3) larvae, which was designated as A. suum C-type lectin-1 (As-CTL-1). The 510 nucleotide open reading frame of As-CTL-1 cDNA encoded the predicted 169 amino acid protein including a ... | 2011 | 22006188 |
| Determinants of nucleotide-binding selectivity of malic enzyme. | Malic enzymes have high cofactor selectivity. An isoform-specific distribution of residues 314, 346, 347 and 362 implies that they may play key roles in determining the cofactor specificity. Currently, Glu314, Ser346, Lys347 and Lys362 in human c-NADP-ME were changed to the corresponding residues of human m-NAD(P)-ME (Glu, Lys, Tyr and Gln, respectively) or Ascaris suum m-NAD-ME (Ala, Ile, Asp and His, respectively). Kinetic data demonstrated that the S346K/K347Y/K362Q c-NADP-ME was transformed ... | 2011 | 21980421 |
| nucleic acid transfection and transgenesis in parasitic nematodes. | summarytransgenesis is an essential tool for assessing gene function in any organism, and it is especially crucial for parasitic nematodes given the dwindling armamentarium of effective anthelmintics and the consequent need to validate essential molecular targets for new drugs and vaccines. two of the major routes of gene delivery evaluated to date in parasitic nematodes, bombardment with dna-coated microparticles and intragonadal microinjection of dna constructs, draw upon experience with the f ... | 2011 | 21880161 |
| ascaris suum draft genome. | parasitic diseases have a devastating, long-term impact on human health, welfare and food production worldwide. more than two billion people are infected with geohelminths, including the roundworms ascaris (common roundworm), necator and ancylostoma (hookworms), and trichuris (whipworm), mainly in developing or impoverished nations of asia, africa and latin america. in humans, the diseases caused by these parasites result in about 135,000 deaths annually, with a global burden comparable with tha ... | 2011 | 22031327 |
| Salmeterol or doxycycline do not inhibit acute bronchospasm and airway inflammation in cats with experimentally-induced asthma. | The objective of this study was to determine if inhaled salmeterol, a long-acting ß(2)-adrenergic agonist, and oral doxycycline, a tetracycline antibiotic displaying matrix metalloproteinase (MMP) inhibitory activity, reduce airway inflammation and obstruction in cats with experimentally-induced asthma. Eight Ascaris suum (AS)-sensitised cats were enrolled in a prospective study in which they underwent four AS-challenges at 1month intervals. The challenged animals were given no treatment or were ... | 2011 | 22136877 |
| national monitoring of ascaris suum related liver pathologies in english abattoirs: a time-series analysis, 2005-2010. | ascaris suum is the most important internal parasite in farmed pigs world-wide. in england, the bpex pig health scheme (bphs) monitors the prevalence of ascariosis in slaughtered finished pigs by identifying milk spots - the healing lesions caused by a. suum larvae migration through the liver. this study investigates the trend of milk spot lesions from july 2005 to december 2010 to identify the progress made by the industry in controlling this parasitic disease. for visual explorations, the mont ... | 2011 | 21889266 |
| structural basis for nematode eif4e binding an m(2,2,7)g-cap and its implications for translation initiation. | metazoan spliced leader (sl) trans-splicing generates mrnas with an m(2,2,7)g-cap and a common downstream sl rna sequence. the mechanism for eif4e binding an m²²⁷g-cap is unknown. here, we describe the first structure of an eif4e with an m(2,2,7)g-cap and compare it to the cognate m⁷g-eif4e complex. these structures and nuclear magnetic resonance (nmr) data indicate that the nematode ascaris suum eif4e binds the two different caps in a similar manner except for the loss of a single hydrogen bond ... | 2011 | 21965542 |
| Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad. | Ascaris suum (large roundworm of pigs) is a parasitic nematode that causes substantial losses to the meat industry. This nematode is suitable for biochemical studies because, unlike C. elegans, homogeneous tissue samples can be obtained by dissection. It has large sperm, produced in great numbers that permit biochemical studies of sperm motility. Widespread study of A. suum would be facilitated by more comprehensive genome resources and, to this end, we have produced a gonad transcriptome of A. ... | 2011 | 21962222 |
| comparative analyses of the complete mitochondrial genomes of ascaris lumbricoides and ascaris suum from humans and pigs. | ascaris lumbricoides and ascaris suum are parasitic nematodes living in the small intestine of humans and pigs, and can cause the disease ascariasis. for long, there has been controversy as to whether the two ascaridoid taxa represent the same species due to their significant resemblances in morphology. however, the complete mitochondrial (mt) genome data have been lacking for a. lumbricoides in spite of human and animal health significance and socio-economic impact globally of these parasites. ... | 2012 | 22075400 |
| mitochondrial fumarate reductase as a target of chemotherapy: from parasites to cancer cells. | recent research on respiratory chain of the parasitic helminth, ascaris suum has shown that the mitochondrial nadh-fumarate reductase system (fumarate respiration), which is composed of complex i (nadh-rhodoquinone reductase), rhodoquinone and complex ii (rhodoquinol-fumarate reductase) plays an important role in the anaerobic energy metabolism of adult parasites inhabiting hosts. the enzymes in these parasite-specific pathways are potential target for chemotherapy. we isolated a novel compound, ... | 2011 | 22226661 |
| in vitro and in vivo efficacy of monepantel (aad 1566) against laboratory models of human intestinal nematode infections. | few effective drugs are available for soil-transmitted helminthiases and drug resistance is of concern. in the present work, we tested the efficacy of the veterinary drug monepantel, a potential drug development candidate compared to standard drugs in vitro and in parasite-rodent models of relevance to human soil-transmitted helminthiases. | 2011 | 22216366 |
| investigation of parasitic and bacterial diseases in pigs with analysis of hematological and serum biochemical profile. | the present study was undertaken to evaluate various disease conditions prevalent in slaughtered pigs and zoonotic importance. the study was conducted on two hundred non-descript pigs slaughtered at an organized slaughter house, mumbai. the animals included in the study were randomly selected. post mortem examination of the animals was performed to note various disease conditions and tissues were collected for histopathology. direct examination of stool was found negative for parasites. gross an ... | 2011 | 23542948 |
| the effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on bifidobacteria and campylobacter in piglets around weaning. | the restrictions on the use of antibiotic and anthelmintic treatments in organic pig farming necessitate alternative non-medical control strategies. therefore, the antibiotic and parasite-reducing effect of a fructan-rich (prebiotic) diet of dried chicory was investigated in free-ranging piglets. approximately half of 67 piglets from nine litters were experimentally infected with ascaris suum and trichuris suis in the suckling period (1 to 7 weeks of age) and 58 of the piglets were challenged da ... | 2011 | 22440024 |
| three independent techniques localize expression of transcript afp-11 and its bioactive peptide products to the paired avk neurons in ascaris suum : in situ hybridization, immunocytochemistry, and single cell mass spectrometry. | we utilized three independent techniques, immunocytochemistry (icc), single cell mass spectrometry (ms), and in situ hybridization (ish), to localize neuropeptides and their transcripts in the nervous system of the nematode ascaris suum . af11 (sdigisepnflrfa) is an endogenous peptide with potent paralytic effects on a. suum locomotory behavior. a highly specific antibody to af11 showed robust immunostaining for af11 in the paired avk neurons in the ventral ganglion. we traced the processes from ... | 2012 | 23509978 |
| an anomaly within the genital system in a female ascaris suum goeze. | the authors describe an individual of the female ascaris suum goeze with a unique genital system. a female with such an anomaly was found during laboratory classes of invertebrate zoology at the faculty of biology and environmental protection, ncu. the specimen was about 200 mm long, and the total length of the reproductive system was 1970 mm. further comparative analysis between unchanged and changed individuals revealed differences in the length of individual sections of the studied system, as ... | 2012 | 23444801 |
| cloning and characterization of hypoxia-inducible factor-1 subunits from ascaris suum - a parasitic nematode highly adapted to changes of oxygen conditions during its life cycle. | the parasitic nematode ascaris suum successfully adapts to a significant decrease in oxygen availability during its life cycle by altering its metabolic system dramatically. however, little is known about the regulatory mechanisms of adaptation to hypoxic environments in a. suum. in multicellular organisms, hypoxia-inducible factor-1 (hif-1), a heterodimeric transcription factor composed of hif-1α and hif-1β subunits, is a master regulator of genes involved in adaptation to hypoxia. in the prese ... | 2012 | 23268347 |
| survival of weed seeds and animal parasites as affected by anaerobic digestion at meso- and thermophilic conditions. | anaerobic digestion of residual materials from animals and crops offers an opportunity to simultaneously produce bioenergy and plant fertilizers at single farms and in farm communities where input substrate materials and resulting digested residues are shared among member farms. a surplus benefit from this practice may be the suppressing of propagules from harmful biological pests like weeds and animal pathogens (e.g. parasites). in the present work, batch experiments were performed, where survi ... | 2012 | 23266071 |
| a globin domain in a neuronal transmembrane receptor of caenorhabditis elegans and ascaris suum: molecular modeling and functional properties. | we report the structural and biochemical characterization of glb-33, a putative neuropeptide receptor that is exclusively expressed in the nervous system of the nematode caenorhabditis elegans. this unique chimeric protein is composed of a 7-transmembrane domain (7tm), glb-33 7tm, typical of a g-protein-coupled receptor, and of a globin domain (gd), glb-33 gd. comprehensive sequence similarity searches in the genome of the parasitic nematode, ascaris suum, revealed a chimeric protein that is sim ... | 2015 | 25666609 |
| duplex quantitative real-time pcr assay for the detection and discrimination of the eggs of toxocara canis and toxocara cati (nematoda, ascaridoidea) in soil and fecal samples. | toxocarosis is a zoonotic disease caused by toxocara canis (t. canis) and/or toxocara cati (t. cati), two worldwide distributed roundworms which are parasites of canids and felids, respectively. infections of humans occur through ingestion of embryonated eggs of t. canis or t. cati, when playing with soils contaminated with dogs or cats feces. accordingly, the assessment of potential contamination of these areas with these roundworms eggs is paramount. | 2012 | 23216873 |
| beta carbonic anhydrases: novel targets for pesticides and anti-parasitic agents in agriculture and livestock husbandry. | the genomes of many insect and parasite species contain beta carbonic anhydrase (β-ca) protein coding sequences. the lack of β-ca proteins in mammals makes them interesting target proteins for inhibition in treatment of some infectious diseases and pests. many insects and parasites represent important pests for agriculture and cause enormous economic damage worldwide. meanwhile, pollution of the environment by old pesticides, emergence of strains resistant to them, and their off-target effects a ... | 2014 | 25174433 |
| characterization of the ca2+-gated and voltage-dependent k+-channel slo-1 of nematodes and its interaction with emodepside. | the cyclooctadepsipeptide emodepside and its parent compound pf1022a are broad-spectrum nematicidal drugs which are able to eliminate nematodes resistant to other anthelmintics. the mode of action of cyclooctadepsipeptides is only partially understood, but involves the latrophilin lat-1 receptor and the voltage- and calcium-activated potassium channel slo-1. genetic evidence suggests that emodepside exerts its anthelmintic activity predominantly through slo-1. indeed, slo-1 deficient caenorhabdi ... | 2014 | 25521608 |
| serine proteases of parasitic helminths. | serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. a number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development and nutrition, host tissues and cell invasion, anticoagulation, and immune evasion. in this review, we de ... | 2015 | 25748703 |
| are ascaris lumbricoides and ascaris suum a single species? | since the original description and naming of ascaris lumbricoides from humans by linnaeus in 1758 and later of ascaris suum from pigs by goeze 1782, these species have been considered to be valid. four hypotheses relative to the conspecificity or lack thereof (and thus origin of these species) are possible: 1) ascaris lumbricoides (usually infecting humans) and ascaris suum (recorded mostly from pigs) are both valid species, with the two species originating via a speciation event from a common a ... | 2012 | 22348306 |
| cloning and characterization of a novel sigma-like glutathione s-transferase from the giant panda parasitic nematode, baylisascaris schroederi. | baylisascaris schroederi, an intestinal nematode of the giant panda, is the cause of the often fatal disease, baylisascariasis. glutathione s-transferases (gsts) are versatile enzymes that can affect parasite survival and parasite-host interactions and, are therefore, potential targets for the development of diagnostic tests and vaccines. | 2015 | 25613998 |
| functional and phylogenetic characterization of proteins detected in various nematode intestinal compartments. | the parasitic nematode intestine is responsible for nutrient digestion and absorption, and many other processes essential for reproduction and survival, making it a valuable target for anthelmintic drug treatment. however, nematodes display extreme biological diversity (including occupying distinct trophic habitats), resulting in limited knowledge of intestinal cell/protein functions of fundamental or adaptive significance. we developed a perfusion model for isolating intestinal proteins in asca ... | 2015 | 25609831 |
| peptidases compartmentalized to the ascaris suum intestinal lumen and apical intestinal membrane. | the nematode intestine is a tissue of interest for developing new methods of therapy and control of parasitic nematodes. however, biological details of intestinal cell functions remain obscure, as do the proteins and molecular functions located on the apical intestinal membrane (aim), and within the intestinal lumen (il) of nematodes. accordingly, methods were developed to gain a comprehensive identification of peptidases that function in the intestinal tract of adult female ascaris suum. peptid ... | 2015 | 25569475 |
| proteomic analysis of the excretory-secretory products from larval stages of ascaris suum reveals high abundance of glycosyl hydrolases. | ascaris lumbricoides and ascaris suum are socioeconomically important and widespread parasites of humans and pigs, respectively. the excretory-secretory (es) molecules produced and presented at the parasite-host interface during the different phases of tissue invasion and migration are likely to play critical roles in the induction and development of protective immune and other host responses. | 2013 | 24098821 |
| sensitivity and efficiency of selected coproscopical methods-sedimentation, combined zinc sulfate sedimentation-flotation, and mcmaster method. | coproscopical methods used in veterinary-parasitological diagnostics were validated according to their sensitivity (se) and egg recovery rate [efficiency (ef)]. validation of the combined sedimentation-flotation method and the modified mcmaster method was performed by using feces spiked with eggs of ancylostoma caninum, uncinaria stenocephala, cooperia oncophora, cyathostomins, ascaris suum, toxascaris leonina, toxocara canis, trichuris vulpis, moniezia expansa, and anoplocephala perfoliata. for ... | 2016 | 26997342 |
| detailed transcriptome description of the neglected cestode taenia multiceps. | the larval stage of taenia multiceps, a global cestode, encysts in the central nervous system (cns) of sheep and other livestock. this frequently leads to their death and huge socioeconomic losses, especially in developing countries. this parasite can also cause zoonotic infections in humans, but has been largely neglected due to a lack of diagnostic techniques and studies. recent developments in next-generation sequencing provide an opportunity to explore the transcriptome of t. multiceps. | 2012 | 23049872 |
| microfluidic platform for electrophysiological recordings from host-stage hookworm and ascaris suum larvae: a new tool for anthelmintic research. | the screening of candidate compounds and natural products for anthelmintic activity is important for discovering new drugs against human and animal parasites. we previously validated in caenorhabditis elegans a microfluidic device ('chip') that records non-invasively the tiny electrophysiological signals generated by rhythmic contraction (pumping) of the worm's pharynx. these electropharyngeograms (epgs) are recorded simultaneously from multiple worms per chip, providing a medium-throughput read ... | 2016 | 27751868 |
| molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm ancylostoma ceylanicum. | nematodes are unable to synthesize fatty acids de novo and must acquire them from the environment or host. it is hypothesized that two unique classes of fatty acid and retinol binding proteins that nematodes produce (fatty acid and retinol binding (far) and nematode polyprotein antigen/allergen (npa)) are used to meet this need. a partial cdna has been cloned corresponding to four subunits of a putative ancylostoma ceylanicum npa (acenpa). the translated amino acid sequence of acenpa shares sequ ... | 2014 | 25481749 |
| molecular cloning and analysis of ancylostoma ceylanicum glutamate-cysteine ligase. | glutamate-cysteine ligase (gcl) is a heterodimer enzyme composed of a catalytic subunit (gclc) and a modifier subunit (gclm). this enzyme catalyses the synthesis of γ-glutamylcysteine, a precursor of glutathione. cdnas of the putative glutamate-cysteine ligase catalytic (ace-gclc) and modifier subunits (ace-gclm) of ancylostoma ceylanicum were cloned using the race-pcr amplification method. the ace-gclc and ace-gclm cdnas encode proteins with 655 and 254 amino acids and calculated molecular mass ... | 2014 | 25092620 |
| evaluation of the use of c-terminal part of the schistosoma mansoni 200kda tegumental protein in schistosomiasis diagnosis and vaccine formulation. | schistosoma mansoni tegument is involved in essential functions for parasite survival and represents a target for screening candidates for vaccine and diagnosis. our group using reverse vaccinology selected six candidates, previously demonstrated by proteomics studies to be expressed in the parasite tegument, among them was sm200. in this work we have cloned and expressed a recombinant form of sm200 c-terminal (1069-1520) region. the efficacy of rsm200 (1069-1520) in the diagnosis of schistosomi ... | 2014 | 24560833 |
| draft genome of neurotropic nematode parasite angiostrongylus cantonensis, causative agent of human eosinophilic meningitis. | angiostrongylus cantonensis is a bursate nematode parasite that causes eosinophilic meningitis (or meningoencephalitis) in humans in many parts of the world. the genomic data from a. cantonensis will form a useful resource for comparative genomic and chemogenomic studies to aid the development of diagnostics and therapeutics. we have sequenced, assembled and annotated the genome of a. cantonensis. the genome size is estimated to be ∼260 mb, with 17,280 genomic scaffolds, 91x coverage, 81.45% for ... | 2015 | 25910624 |
| trichinella spiralis: monoclonal antibody against the muscular larvae for the detection of circulating and fecal antigens in experimentally infected rats. | in this work we search for antigens of trichinella spiralis in sera and stool of rats experimentally infected. the kinetic of antibodies to excretory and secretory (es) antigens of muscle larvae (ml) was also determined. wistar rats were infected with 15 ml per gram of body weight and blood samples were collected weekly for 10 weeks. antibodies were studied using an indirect elisa. for detection of circulating antigens and coproantigens, a sandwich elisa was developed with the use of polyclonal ... | 2012 | 23026455 |
| haemoglobin, a new major allergen of anisakis simplex. | gastro-allergic anisakiasis and anisakis sensitisation associated chronic urticaria are diseases which differ in their ige and igg4 responses against both crude extract and specific allergens. anisakis and ascaris are closely related nematodes that usually cause problems with specificity in immunodiagnostics. in this study we measured ige and igg4 antibodies against anisakis simplex sensu lato (s. l.) and ascaris suum haemoglobins in sera of 21 gastro-allergic anisakiasis and 23 chronic urticari ... | 2015 | 25683373 |