| spatiotemporal expression and substrate specificity analysis of the cucumber sweet gene family. | the functions of sweet (sugar will eventually be exported transporter) proteins have been studied in a number of crops, but little is known about their roles in cucumber (cucumis sativus l.), a model plant for studying stachyose metabolism and phloem function. here, we identified 17 cucumber sweet genes (cssweets), located on chromosomes 1-6, and classified them into four clades. two genes from each clade were selected for spatiotemporal expression, subcellular localization, and substrate specif ... | 2017 | 29163584 |
| impact of postharvest exogenous γ-aminobutyric acid treatment on cucumber fruit in response to chilling tolerance. | low-temperature storage is generally used to extend postharvest lifetime and to inhibit decay of cucumber fruit, but it also enhances the intensity of chilling injury. the capability of γ-aminobutyric acid to enhance antioxidant enzyme activities and reduce chilling injury was studied in cucumber (cucumis sativus l.) fruit stored at 1 °c for 5 weeks. the purpose of this study was to define if the gaba-induced modification in antioxidant system and phospholipase activity is linked to the reduced ... | 2017 | 29158632 |
| phytotoxicity of pyrethroid pesticides and its metabolite towards cucumis sativus. | pyrethroid pesticides residues have been frequently detected in soils and have been recognized to contribute to soil toxicity. the phytotoxic impact of pesticides was evaluated in cucumis sativus (c. sativus) seeds. percentage of seed germination, root elongation, shoot length and leaf length were considered as endpoints to assess the possible acute phytotoxicity of soil by the exposure to pyrethroid pesticides (cypermethrin, deltamethrin and cyhalothrin) and its metabolite phenoxybenzoic acid ( ... | 2017 | 29156286 |
| effects of exogenous application of cppu, naa and ga4+7 on parthenocarpy and fruit quality in cucumber (cucumis sativus l.). | in protected vegetable fields, plant growth regulators are often used to improve cucumber fruit growth. however, the effects of plant growth regulators on the appearance and nutritional quality of cucumber (cucumis sativus l.) remain largely unknown. in the present study, 100 mg/l n-(2-chloro-4-pyridyl)-n'-phenylurea (cppu), naphthaleneacetic acid (naa) or gibberellin a4+a7 (ga4+7) was applied to the female cucumber flowers 1 day before anthesis and at anthesis. the cppu, naa and ga4+7 treatment ... | 2018 | 29146357 |
| fine mapping of a male sterility gene ms-3 in a novel cucumber (cucumis sativus l.) mutant. | the cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an mmd1 -like gene csa3m006660 that may be responsible for the male sterile in cucumber. a cucumber (cucumis sativus l.) male sterile mutant (ms-3) in an advanced-generation inbred line was identified, and genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, ms-3, which was stably inherited. histological studies suggested that the main cause of the male sterility was ... | 2018 | 29134240 |
| identification and mapping of ts (tender spines), a gene involved in soft spine development in cucumis sativus. | using map-based cloning of ts gene, we identified a new sort of gene involved in the initiation of multicellular tender spine in cucumber. the cucumber (cucumis sativus l.) fruit contains spines on the surface, which is an extremely valuable quality trait affecting the selection of customers. in this study, we elaborated cucumber line nc072 with wild type (wt) hard fruit spines and its spontaneous mutant nc073, possessing tender and soft spines on fruits. the mutant trait was named as tender spi ... | 2018 | 29116330 |
| a valid name for the xishuangbanna gourd, a cucumber with carotene-rich fruits. | herbarium specimens deposited in publicly accessible collections are the basis for all scientific names because only permanent specimens can be re-studied by independent researchers, the very essence of science. re-investigations may be done with morphological, chemical, genomic, computer-tomographic, or other methods. based on new herbarium material, i here provide a name for the xishuangbanna gourd, a plant long cultivated in yunnan because of its large non-bitter fruits, rich in β-carotene. g ... | 2017 | 29033662 |
| high light aggravates functional limitations of cucumber canopy photosynthesis under salinity. | most crop species are glycophytes, and salinity stress is one of the most severe abiotic stresses reducing crop yields worldwide. salinity affects plant architecture and physiological functions by different mechanisms, which vary largely between crop species and determine the susceptibility or tolerance of a crop species to salinity. | 2017 | 29028871 |
| calcium and calmodulin are involved in nitric oxide-induced adventitious rooting of cucumber under simulated osmotic stress. | osmotic stress is a major form of abiotic stress that adversely affects growth and development of plants and subsequently reduces yield and quality of crops. in this study, the effect of nitric oxide (no) and calcium (ca2+) on the process of adventitious rooting in cucumber (cucumis sativus l.) under simulated osmotic stress was investigated. the results revealed that the effect of exogenous no and ca2+ in promoting the development of adventitious roots in cucumber seedlings under simulated osmo ... | 2017 | 29021804 |
| methyl jasmonate-induced emission of biogenic volatiles is biphasic in cucumber: a high-resolution analysis of dose dependence. | methyl jasmonate (meja) is a key airborne elicitor activating jasmonate-dependent signaling pathways, including induction of stress-related volatile emissions, but how the magnitude and timing of these emissions scale with meja dose is not known. treatments with exogenous meja concentrations ranging from mild (0.2 mm) to lethal (50 mm) were used to investigate quantitative relationships among meja dose and the kinetics and magnitude of volatile release in cucumis sativus by combining high-resolu ... | 2017 | 28981785 |
| overexpression of cslea11, a y3sk2-type dehydrin gene from cucumber (cucumis sativus), enhances tolerance to heat and cold in escherichia coli. | as the group ii lea (late embryogenesis abundant) proteins, dehydrins (dhns) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. in this study, a dhn gene named cslea11 was identified and characterized from cucumis sativus. sequence analysis of cslea11 showed that it is a y3sk2-type dhn protein rich in hydrophilic amino acids. expression analyses revealed that the transcription of cslea11 could be significantly induced by heat an ... | 2017 | 28963660 |
| genetic diversity and population structure of cucumis sativus l. by using ssr markers. | the genetic variation, marker attributes and population structure was assessed in 104 genotypes of cucumber using 23 ssr primer pairs. the total number of alleles produced was 67 with an average of 2.91 per locus. allele frequency was in the range of 0.215 to 0.561 with mean value of 0.403, polymorphic information content ranged from 0.158 to 0.495 with the mean of 0.333, marker index ranged from 0.316 to 1.54 with an average value of 0.954 and resolving power ranged from 0.346 to 2.692 with mea ... | 2017 | 28955604 |
| response of a predatory ant to volatiles emitted by aphid- and caterpillar-infested cucumber and potato plants. | in response to herbivory by insects, various plants produce volatiles that attract enemies of the herbivores. although ants are important components of natural and agro-ecosystems, the importance of herbivore-induced plant volatiles (hipvs) as cues for ants for finding food sources have received little attention. we investigated responses of the ant formica pratensis to volatiles emitted by uninfested and insect-infested cucumber (cucumis sativus) and potato (solanum tuberosum) plants. cucumber ... | 2017 | 28951999 |
| molecular characterization, expression analysis and heterologous expression of two translationally controlled tumor protein genes from cucumis sativus. | the translationally controlled tumor protein (tctp) is a family of abundant and ubiquitous proteins involved in several important primary functions. cucumbers harbor two tctp genes, cstctp1 and cstctp2; however, their functional roles remain unclear. in this study, we isolated cstctp1 and cstctp2 (xp-004134215 and xp-004135602, respectively) promoters, full-length cdna and genomic sequences from cucumis sativus. bioinformatics analysis, containing cis-acting elements, structural domains, phyloge ... | 2017 | 28926624 |
| bioremoval of zinc and manganese by bacterial biofilm: a bioreactor-based approach. | industrialization has led to the disposal of a massive amount of heavy metals every year, showing perilous effects on humans, marine life and agricultural products. there are numerous chemical and biological approaches available but their implementation is limited due to high cost and low efficiency. therefore, the present study was focused on the biofilm- based bioremoval of heavy metals (zinc and manganese) using indigenous bacteria isolated from the tannery sludge obtained from ranipet, tamil ... | 2017 | 28915490 |
| wilted cucumber plants infected by fusarium oxysporum f. sp. cucumerinum do not suffer from water shortage. | fusarium wilt is primarily a soil-borne disease and results in yield loss and quality decline in cucumber (cucumis sativus). the main symptom of fusarium wilt is the wilting of entire plant, which could be caused by a fungal toxin(s) or blockage of water transport. to investigate whether this wilt arises from water shortage, the physiological responses of hydroponically grown cucumber plants subjected to water stress using polyethylene glycol (peg, 6000) were compared with those of plants infect ... | 2017 | 28911018 |
| the expression of a candidate cucumber fruit sugar starvation marker gene cssef1 is enhanced in malformed fruit induced by salinity. | the cucumber (cucumis sativus l.) gene cucumis sativus somatic embryogenesis zinc finger 1 (cssef1) was suggested to be a good marker gene for sugar starvation in fruit. the expression of this gene in fruits is dramatically upregulated in plants that have suffered either complete defoliation or prolonged darkness. cssef1 was initially discovered as a gene that was upregulated during somatic embryogenesis. we examined the difference in fruit parts and the effect of pollination on the upregulation ... | 2017 | 28878495 |
| interactions between aluminium, iron and silicon in cucumber sativus l. grown under acidic conditions. | aluminium (al) is one of the major stressors for plants in acidic soils, negatively affecting plant growth and nutrient balances. significant efforts have been undertaken to understand mechanisms of al tolerance in plants. however, little is known of the relevance of iron (fe) and silicon (si) nutrition under al stress conditions. the objectives of this study were to determine whether effects induced by fe and si are of importance for limitation of al moving via xylem in plants (cucumis sativus ... | 2017 | 28818756 |
| genome-wide identification and transcriptional expression analysis of cucumber superoxide dismutase (sod) family in response to various abiotic stresses. | superoxide dismutase (sod) proteins are widely present in the plant kingdom and play important roles in different biological processes. however, little is known about the sod genes in cucumber. in this study, night sod genes were identified from cucumber (cucumis sativus) using bioinformatics-based methods, including 5 cu/znsods, 3 fesods, and 1 mnsod. gene structure and motif analysis indicated that most of the sod genes have relatively conserved exon/intron arrangement and motif composition. p ... | 2017 | 28808654 |
| differential scanning calorimetric and spectroscopic studies on the thermal and chemical unfolding of cucumber (cucumis sativus) phloem exudate lectin. | in plants, chitooligosaccharide-binding phloem exudate lectins play an important role in the defense mechanism against parasites. here, we investigated the thermal and chaotrope-induced unfolding of cucumber (cucumis sativus) phloem exudate lectin (cpl). circular dichroism (cd) spectroscopic studies indicate that the secondary and tertiary structures of cpl are essentially unaltered up to 90°c. consistent with this, differential scanning calorimetric studies revealed that cpl is highly thermosta ... | 2018 | 28778525 |
| 24-epibrassinolide alleviates organic pollutants-retarded root elongation by promoting redox homeostasis and secondary metabolism in cucumis sativus l. | environmental pollution by organic pollutants (ops) has become a global concern due to its detrimental effects on the environment and human health. as plants are used to remediate contaminated sites, understanding the responses of plants to various ops and fortification of plant tolerance are of great significance. in this work, we studied the biochemical and molecular responses of cucumber plants to three well-known ops, 2,4,6-trichlorophenol, chlorpyrifos and oxytetracycline in the absence or ... | 2017 | 28774551 |
| new insights into the roles of cucumber tir1 homologs and mir393 in regulating fruit/seed set development and leaf morphogenesis. | tir1-like proteins act as auxin receptors and play essential roles in auxin-mediated plant development processes. the number of auxin receptor family members varies among species. while the functions of auxin receptor genes have been widely studied in arabidopsis, the distinct functions of cucumber (cucumis sativus l.) auxin receptors remains poorly understood. to further our understanding of their potential role in cucumber development, two tir1-like genes were identified and designated cstir1 ... | 2017 | 28747179 |
| comparative rna-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation. | waterlogging is a serious abiotic stress to plant growth because it results in the decline in the supplement of oxygen to submerged tissues. although cucumber (cucumis sativus l.) is sensitive to waterlogging, its ability to generate adventitious roots (ars) facilitates gas diffusion and increases plant survival when the oxygen concentration is decreased. to gain a better understanding of the molecular mechanisms that enable de novo ar primordia emergence upon waterlogging, the rna sequencing-ba ... | 2017 | 28747176 |
| gravitropism interferes with hydrotropism via counteracting auxin dynamics in cucumber roots: clinorotation and spaceflight experiments. | roots of land plants show gravitropism and hydrotropism in response to gravity and moisture gradients, respectively, for controlling their growth orientation. gravitropism interferes with hydrotropism, although the mechanistic aspects are poorly understood. here, we differentiated hydrotropism from gravitropism in cucumber roots by conducting clinorotation and spaceflight experiments. we also compared mechanisms regulating hydrotropism and auxin-regulated gravitropism. clinorotated or microgravi ... | 2017 | 28722158 |
| identification of all homoeologous chromosomes of newly synthetic allotetraploid cucumis × hytivus and its wild parent reveals stable subgenome structure. | allopolyploidy and homoeologous recombination are two important processes in reshaping genomes and generating evolutionary novelties. newly formed allopolyploids usually display chromosomal perturbations as a result of pairing errors at meiosis. to understand mechanisms of stabilization of allopolyploid species derived from distant chromosome bases, we investigated mitotic stability of a synthetic cucumis allotetraploid species in relation to meiosis chromosome behavior. the cucumis × hytivus is ... | 2017 | 28688040 |
| expression analysis of argonaute, dicer-like, and rna-dependent rna polymerase genes in cucumber (cucumis sativus l.) in response to abiotic stress. | posttranscriptional control of gene expression can be achieved through rna interference when the activities of dicer-like (dcl), argonaute (ago) and rna-dependent rna polymerase (rdr) proteins are significant. in this study, we analysed the expression of seven ago, five dcl and eight rdr genes in cucumber under cold, heat, hormone, salinity and dehydration treatments using quantitative reverse-transcription pcr (qrt-pcr). all csago, csdcl and csrdr genes were differentially expressed under abiot ... | 2017 | 28674223 |
| variation in cucumber (cucumis sativus l.) fruit size and shape results from multiple components acting pre-anthesis and post-pollination. | morphological, qtl, and gene expression analyses indicate variation in cucumber fruit size and shape results from orientation, timing, and extent of cell division and expansion, and suggest candidate gene factors. variation in cucumber (cucumis sativus l.) fruit size and shape is highly quantitative, implicating interplay of multiple components. recent studies have identified numerous fruit size and shape quantitative trait loci (qtl); however, underlying factors remain to be determined. we exam ... | 2017 | 28623561 |
| suppression of cucumber stachyose synthase gene (cssts) inhibits phloem loading and reduces low temperature stress tolerance. | stachyose is the main transporting sugar in phloem of raffinose family oligosaccharides-transporting species. stachyose synthase (sts) is a key enzyme for stachyose biosynthesis, but the gene encoding sts is poorly characterized in cucumber (cucumis sativus l.), which is a model plant for studying stachyose metabolism and phloem function. in this research, stachyose synthase gene (cssts) from cucumber was isolated and its physiological functions were analyzed. cssts expressed mainly in the phloe ... | 2017 | 28608281 |
| solid-state fermentation for production of a bioherbicide from diaporthe sp. and its formulation to enhance the efficacy. | in this study, a bioherbicide was produced by solid-state fermentation (ssf) using diaporthe sp. adjuvants were employed in a formulation to enhance the herbicidal activity towards the target (cucumis sativus). the study was divided into two steps: (1) the fermentation condition for bioherbicide production was assessed; (2) evaluation of different formulations containing palm oil, tween® 80 and span® 80, in order to increase phytotoxicity. in step 1, the maximum herbicidal activity (1.23% of the ... | 2017 | 28593519 |
| comparative study of lectin domains in model species: new insights into evolutionary dynamics. | lectins are present throughout the plant kingdom and are reported to be involved in diverse biological processes. in this study, we provide a comparative analysis of the lectin families from model species in a phylogenetic framework. the analysis focuses on the different plant lectin domains identified in five representative core angiosperm genomes (arabidopsisthaliana, glycine max, cucumis sativus, oryza sativa ssp. japonica and oryza sativa ssp. indica). the genomes were screened for genes enc ... | 2017 | 28587095 |
| procoagulant serine glycoprotease from cucumis sativus l.: action on human fibrinogen and fibrin clot. | upon examination of the fruit extract of cucumis sativus l. for its pharmacological benefits, it was previously observed that it has potential proteolytic, fibrinogenolytic and procoagulant activities. these properties can be attributed to the presence of the protease. in this regard, the present study comprised of purification and characterization of protease. purification of the enzyme involved ammonium sulfate precipitation followed by gel filtration and ion exchange chromatography. the purif ... | 2017 | 28555432 |
| overexpression of the rubisco activase gene improves growth and low temperature and weak light tolerance in cucumis sativus. | rubisco activase (rca) is an important enzyme that can catalyze the carboxylation and oxygenation activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), which is involved in the photosynthetic carbon reduction cycle. here, we studied the effects of changes in rca activity on photosynthesis, growth and development, as well as the low temperature and weak light tolerance of rca overexpressing transgenic cucumber (cucumis sativus) plants. csrca overexpression increased the plant h ... | 2017 | 28543370 |
| the solanum tuberosum kst1 partial promoter as a tool for guard cell expression in multiple plant species. | to date, guard cell promoters have been examined in only a few species, primarily annual dicots. a partial segment of the potato (solanum tuberosum) kst1 promoter (kst1 partial promoter, kst1ppro) has previously been shown to confer guard cell expression in potato, tomato (solanum lycopersicum), citrus [troyer citrange (c. sinensis×poncirus trifoliata)], and arabidopsis (arabidopsis thaliana). here, we describe an extensive analysis of the expression pattern of kst1ppro in eight (previously repo ... | 2017 | 28531314 |
| a mutant in the csdet2 gene leads to a systemic brassinosteriod deficiency and super compact phenotype in cucumber (cucumis sativus l.). | a novel dwarf cucumber mutant, scp-2, displays a typical br biosynthesis-deficient phenotype, which is due to a mutation in csdet2 for a steroid 5-alpha-reductase. brassinosteroids (brs) are a group of plant hormones that play important roles in the development of plant architecture, and extreme dwarfism is a typical outcome of br-deficiency. most cucumber (cucumis sativus l.) varieties have an indeterminate growth habit, and dwarfism may have its value in manipulation of plant architecture and ... | 2017 | 28516384 |
| adapting the vegetative vigour terrestrial plant test for assessing ecotoxicity of aerosol samples. | plants, being recognized to show high sensitivity to air pollution, have been long used to assess the ecological effects of airborne contaminants. however, many changes in vegetation are now generally attributed to atmospheric deposition of aerosol particles; the dose-effect relationships of this process are usually poorly known. in contrast to bioindication studies, ecotoxicological tests (or bioassays) are controlled and reproducible where ecological responses are determined quantitatively. in ... | 2017 | 28502046 |
| the association of changes in dna methylation with temperature-dependent sex determination in cucumber. | cucumber (cucumis sativus l.) is characterized by its diverse and flexible sexual types. here, we evaluated the effect of low temperature (lt) exposure on cucumber femaleness under short-day conditions. shoot apices were subjected to whole-genome bisulfate sequencing (wgbs), mrna-seq, and srna-seq. the results showed that temperature had a substantial and global impact on transposable element (te)-related small rna-directed dna methylation (rddm) mechanisms, resulting in large amounts of chh-typ ... | 2017 | 28498935 |
| unravelling cadmium toxicity and nitric oxide induced tolerance in cucumis sativus: insight into regulatory mechanisms using proteomics. | nitric oxide (no) is a signal molecule that can mediate a wide range of physiological processes against cadmium (cd) toxicity in plants. however, little information can be used to reveal the global and systematic mitigative mechanism of no in improving cd stress tolerance of cucumber plants. in the present study, we used isobaric tag for relative and absolute quantification (itraq) analysis to identify 1691 proteins, which can be used to determine the role of no in regulating the molecular chang ... | 2017 | 28494308 |
| comparative metabolic response between cucumber (cucumis sativus) and corn (zea mays) to a cu(oh)2 nanopesticide. | due to their unique properties, copper-based nanopesticides are emerging in the market. thus, understanding their effect on crop plants is very important. metabolomics can capture a snapshot of cellular metabolic responses to a stressor. we selected maize and cucumber as model plants for exposure to different doses of cu(oh)2 nanopesticide. gc-tof-ms-based metabolomics was employed to determine the metabolic responses of these two species. results revealed significant differences in metabolite p ... | 2017 | 28493687 |
| fine mapping identifies csgcn5 encoding a histone acetyltransferase as putative candidate gene for tendril-less1 mutation (td-1) in cucumber. | next-generation sequencing-aided map-based cloning delimited the cucumber tendril - less1 ( td - 1 ) locus into a 190.7-kb region in chromosome 6 harboring a putative, novel-function candidate gene encoding a histone acetyltransferase ( csgcn5 ). the tendril initiated from the lateral meristem is an important and characteristic organ for the species in the cucurbitaceae family including cucumber (cucumis sativus l.). while the tendril has its evolutionary significance, it also poses a nuisance i ... | 2017 | 28466109 |
| qtl mapping of domestication and diversifying selection related traits in round-fruited semi-wild xishuangbanna cucumber (cucumis sativus l. var. xishuangbannanesis). | qtl analysis revealed 11 qtl underlying flowering time and fruit size variation in the semi-wild xishuangbanna cucumber, of which, ft6.2 and fs5.2 played the most important roles in determining photoperiod-dependent flowering time and round-fruit shape, respectively. flowering time and fruit size are two important traits in domestication and diversifying selection in cucumber, but their genetic basis is not well understood. here we reported qtl mapping results on flowering time and fruit size wi ... | 2017 | 28439621 |
| effect of short-term water deficit stress on antioxidative systems in cucumber seedling roots. | cucumber is one of the most popular vegetables, and have little tolerance for water stress. the antioxidant defense system is one of major drought defense and adaptive mechanisms in plants, however, relatively few data are available regarding antioxidant systems in responses of cucumber to water deficit. the effect of short-term drought stress on the antioxidant system, lipid peroxidation and water content in cucumber seedlings roots was investigated. | 2014 | 28510935 |
| temporal shifts in gene flow and seed set: evidence from an experimental population of cucumis sativus. | | 1984 | 28563773 |
| contrasting gene flow patterns and genetic subdivision in adjacent populations of cucumis sativus (cucurbitaceae). | | 1983 | 28568124 |