| a trial of nonspecific immunotherapy using systemic c. parvum in treated patients with dukes b and c colorectal cancer. | in view of the relatively poor prognosis for patients after surgery for locally invasive colorectal cancer a trial of repeated systemic infusions of corynebacterium parvum (cp) has been carried out. it is in this group of patients, with a high risk of recurrence from small residues of cancer left by the surgeon, that immunotherapy should have its optimum chance of success. a total of 92 patients were included in a randomized controlled study. the two groups were comparable in terms of tumour sta ... | 1982 | 7041943 |
| actinomycin-d plus 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamine (dtic) with or without intravenous corynebacterium parvum in metastatic malignant melanoma. | chemotherapy and chemoimmunotherapy of stage iv b malignant melanoma were compared in 88 patients. chemotherapy consisted of dtic 250 mg/m2 of the body surface area daily x five days and actinomycin-d 2 mg/m2 on day 1 repeated every 3--4 weeks. chemoimmunotherapy consisted of the same regimen plus c. parvum 2 mg/m2 i.v. daily for 14 days before every third cycle of chemotherapy, plus 2 mg/m2 i.v. daily on days 7 and 14 of each 21--28 day chemotherapy cycle. there was 32 evaluable chemotherapy an ... | 1982 | 7042068 |
| generation of tumor cells with reduced dna content as a result of macrophage tumoricidal activity. | | 1982 | 7042078 |
| regulation of the expression of adoptive tumor rejection immunity by recipient cyclophosphamide-sensitive cells. | peritoneal exudate t-cells from rats immune to 13762a rat mammary tumor conferred specific tumor rejection immunity on normal recipients. the efficiency of systemic adoptive transfer of tumor rejection immunity with immune peritoneal exudate t-cells was improved by cyclophosphamide (cy) pretreatment of recipients. optimal potentiation was obtained with a dose of 50 or 100 mg cy per kg body weight given the day before transfer. cy pretreatment of recipients was effective 1 to 3 days prior to tran ... | 1982 | 7042079 |
| distribution of igm, iga and igg secreting cells in the tissues of normal and tumour-bearing mice. | the levels of igm, iga and igg secreting cells were examined in control, corynebacterium parvum-stimulated and tumour-bearing, normal and athymic (nu/nu) mice. the percentage of iga to igm or igg secreting cells is relatively higher in peripheral blood than in the spleen or peritoneum of normal mice. within tumours, irrespective of their degree of vascularization and immunogenicity, the pattern of ig secreting cells in similar to that seen in peripheral blood and different from that in spleen an ... | 1982 | 7042550 |
| corynebacterium parvum and metastatic breast cancer. | | 1982 | 7044404 |
| a comparison of the toxicity of two formulations of corynebacterium parvum in a double-blind crossover trial in patients with bronchogenic carcinoma. | | 1981 | 7044635 |
| quantitative pyrolysis gas chromatography-mass spectrometry of bacterial cell walls. | | 1982 | 7046504 |
| suppression of polymorphonuclear leukocyte chemotactic factor production in propionibacterium acnes by subminimal inhibitory concentrations of tetracycline, ampicillin, minocycline, and erythromycin. | propionibacterium acnes is the cause of inflammation in acne vulgaris and has been shown to produce potent neutrophil chemoattractants. different strain of p. acnes that were sensitive or resistant to ampicillin, erythromycin, minocycline, and tetracycline were grown in the presence of subminimal inhibitory concentrations of the drugs, and their culture supernatants were assayed for neutrophil chemotactic activity. the presence of subminimal inhibitory concentrations of ampicillin failed to affe ... | 1982 | 7049078 |
| effects of propionibacterium acnes treatment on the course of mycobacterium leprae infection in mice. | studies were carried out to determine the effects of treatment with killed suspensions of propionibacterium acnes (formerly designated corynebacterium parvum) on the course of mycobacterium leprae infection in mice. systemic (intravenous or intraperitoneal) treatment with p. acnes failed to significantly alter the growth of m. leprae in the mouse footpad. in contrast, injections of p. acnes directly into the infected footpad markedly inhibited the growth of the leprosy bacilli regardless of whet ... | 1982 | 7049949 |
| genetic control of propionibacterium acnes-induced protection of mice against babesia microti. | using various strains of inbred mice, we found that propionibacterium acnes-induced protection against the hemoprotozoan parasite babesia microti was controlled by a dominant gene(s) not linked to the major histocompatibility gene (h2) complex of mice. p. acnes-induced resistance to infection was not merely an amplification of the normal immune response to b. microti, since innate resistance to infection was controlled by different genes. expression of the nonspecific protection induced by p. ac ... | 1982 | 7054129 |
| neutrophil chemotaxis by propionibacterium acnes lipase and its inhibition. | the chemoattraction of propionibacterium acnes lipase for neutrophils and the effect of lipase inhibitor and two antibiotic agents on the chemotaxis were evaluated. of the various fractions tested, partially purified lipase (fraction 2c) was the most active cytotaxin produced by p. acnes. serum mediators were not required for the generation of chemotaxis by lipase in vitro. diisopropyl phosphofluoridate at low concentration (10(-4) mm) completely inhibited lipase activity as well as polymorphonu ... | 1982 | 7054130 |
| relationship between cell wall synthesis in propionibacterium acnes and ability to stimulate the reticuloendothelial system. | the addition of chloramphenicol or tetracycline to exponentially growing cells of propionibacterium acnes, which are not normally able to induce splenomegaly until they reach stationary phase, resulted in a rapid development of ability to induce splenomegaly in mice. however, the activity resulting from chloramphenicol treatment was arrested by the addition of penicillin or vancomycin, which showed that the activity was dependent on cell wall synthesis. | 1982 | 7056584 |
| a study of reactions to corynebacterium parvum (c. parvum) in the brain and dermis of the rat. | a comparative analysis was made of the histological changes caused by corynebacterium parvum (c. parvum) infected into the brain and dermis of normal and c. parvum presensitized rats. control rats were injected with saline. it was shown that for approximately 3 days after the intracerebral injection the brain reacted with a polymorphonuclear cell infiltration. this reaction was replaced in order by lymphocytes, macrophages, and finally by epithelioid cells progressing to granuloma. the histologi ... | 1982 | 7064658 |
| chronic sclerosing osteomyelitis (garré). | eight patients with chronic sclerosing osteomyelitis (garré) were investigated for an average follow-up of 13 years. the disease is a well definable clinical entity affecting children and young adults. secondary lesions occurred in four cases after an average of 5.5 years. five patients with a history of an average of eight years duration were free of symptoms. whereas the remaining three patients with a history of six, 13, and 15 years, respectively, had intermittent recurrences. the roentgenol ... | 1982 | 7067276 |
| purification and certain properties of a bacteriocin from streptococcus mutans. | an inhibition factor from streptococcus mutans strain c3603 (serotype c) was purified and isolated, and its properties indicated that it was a bacteriocin. bacteriocin c3603 is a basic protein with a pi value of 10 and a molecular weight of 4,800. the activity of this bacteriocin was not affected by ph over a range of 1.0 to 12.0 or by storage at 100 degrees c for 10 min at ph 2.0 to 7.0 or storage at 121 degrees c for 15 min at ph 4.0. pronase; papain, phospholipase c, trypsin, and alpha-amylas ... | 1982 | 7068219 |
| relationship between phagocytic activity of macrophages and delayed footpad reaction against syngeneic testicular cells. | the delayed footpad reaction against syngeneic testicular cells was studied in cyclophosphamide (cy)-pretreated mice whose mononuclear phagocyte system (mps) was blocked with colloidal carbon or stimulated with corynebacterium parvum (c. parvum). the delayed footpad reaction was augmented by mps-blockade, whereas it was depressed by mps-stimulation. it was observed that syngeneic testicular cells were phagocytized and degraded more effectively by c. parvum-stimulated macrophages in vitro and tha ... | 1982 | 7069784 |
| inhibition by the landschütz ascites carcinoma of the granulomatous inflammatory response to c. parvum. | i.p. or i.v. administration of corynebacterium parvum (cp) to mf1 mice induces a generalized inflammatory response, associated with marked hepatosplenomegaly and accompanied by a pronounced granulomatous response in the liver. injection of the landschütz ascites carcinoma (lac) 24 h after cp substantially reduced the intensity of the inflammatory response, and decreased both the frequency and size of the hepatic granulomas, as revealed by morphometric analysis of histological sections. the diffe ... | 1982 | 7073950 |
| effect of clindamycin, erythromycin, lincomycin, and tetracycline on growth and extracellular lipase production by propionibacteria in vitro. | two propionibacteria identified as propionibacterium acnes and propionibacterium granulosum were grown anaerobically in the presence of growth subinhibitory concentrations (0.25 and 0.5 minimal inhibitory concentrations) of clindamycin, erythromycin, lincomycin, and tetracycline. viable counts and assays of extracellular lipase were performed on samples taken at 24-h intervals over a 96-h period. the results showed that lincomycin and clindamycin could inhibit the production of the enzyme by bot ... | 1982 | 7081974 |
| hypercoagulability after immunotherapy with corynebacterium parvum in man. | the effect of an immunotherapy with corynebacterium parvum on the blood coagulation system was investigated in a randomized trial of 18 patients with metastatic breast cancer. all patients received cytostatic therapy. additionally, c. parvum was given intravenously on day 15 of the cytostatic cycle (group i) or on day 1 (group ii) or not at all (group iii). fibrinopeptide a increased within 2 h after intravenous administration of c. parvum in groups i and ii and normalized after 24 h (p less tha ... | 1982 | 7082858 |
| changes in glycosidase activities and surface lectin receptors of guinea-pig alveolar macrophages activated by corynebacterium parvum. | | 1982 | 7083271 |
| in vivo porphyrin fluorescence for propionibacterium acnes. a characterization of the fluorescing pigments. | | 1982 | 7084539 |
| effect of adrenoceptor blockade on hemorrhagic necrosis of meth a sarcomata induced by endotoxin or tumor necrosis serum. | an intravenous injection of endotoxin into balb/c mice bearing subcutaneous meth a sarcomata caused hemorrhagic necrosis and reduced growth of the tumors. in a number of instances this was followed by regression. alpha-adrenergic blockade with phentolamine prior to endotoxin did not influence tumor growth, but tended to reduce the incidence of regression when compared to mice merely treated with endotoxin. the frequency of hemorrhagic necrosis was not changed, although the extent of necrosis was ... | 1982 | 7085258 |
| eight year persistence of individual differences in the bacterial flora of the forehead. | eight subjects having a rich (greater than or equal to 100,000 per cm2) population of propionibacterium acnes on the forehead and 7 with a sparse population (great than or equal to 15,000 per cm2) in earlier tests were reexamined after median interval of 8 yr. all of those with an initial rich population had greater than or equal to 100,000 per cm2 on retest. of the 7 with an initial sparse population, 4 again had fewer than 15,000, one had more than 100,000 per cm2 and 2 were intermediate. thre ... | 1982 | 7086176 |
| streptococcus pneumoniae and three species of anaerobic bacteria in experimental otitis media in guniea pigs. | three species of anaerobic bacteria commonly isolated in upper respiratory tract infections in humans were studied in an animal model of otitis media. the ability of propionibacterium acnes to induce acute otitis media was comparable with that of streptococcus pneumoniae, the classic middle ear pathogen. the response to peptostreptococcus micros was weak and irregular, and no effects of bacteroides asaccharolyticus were observed despite the use of high doses (10(8) colony-forming unite per ear). ... | 1982 | 7086197 |
| [anti-tumour cytostatic and cytotoxic properties of peritoneal exudate cells of conventional and germ-free mice, stimulated or not by "corynebacterium parvum" (author's transl)]. | the treatment by corynebacterium parvum induced an increase of peritoneal cell number in conventional mice but no modifications in germ-free mice. against yc8 tumoral target cells, cytostatic properties of peritoneal cells were of the same intensity in conventional and germ-free after c. parvum treatment. against k.balb cells, c. parvum treatment induced an increase of cytostatic properties from 9 to 93% in conventional mice and from 51 to 84% in germ-free mice. cytotoxic properties were increas ... | 1982 | 7092174 |
| two anti-tumour cytotoxic cells in the peritoneal cavity of rats: natural occurrence, augmentation and partial characterization. | mononuclear cells cytotoxic to methylcholanthrene-induced fibrosarcoma cells were detected in the peritoneal cavities of normal rats by means of 16 h 51cr release and 48 h 125iudr release assays. carriage of a tumour which induces concomitant immunity, or intraperitoneal injections of corynebacterium parvum or proteose-peptone, led to increases in mononuclear cell numbers. injections of c. parvum and proteose-peptone led to increases in cytotoxicity in 51cr release assays. carriage of an immunog ... | 1982 | 7092732 |
| the kinetics of mononuclear phagocytes in normal calves given corynebacterium parvum. | two groups of calves, three in each group, were used to determine the kinetics of mononuclear phagocytes in normal calves and in calves given corynebacterium parvum intravenously, using tritiated thymidine as an in vivo deoxyribonucleic acid label. in normal calves, the mean production time of labelled monocytes in the bone marrow was 36.4 +/- 2.04 hours. the turnover rate of labelled monocytes from the bone marrow into the peripheral blood was 5.4 +/- 0.3% per hour and the disappearance rate of ... | 1982 | 7093810 |
| the cell surface of propionibacterium acnes: effect of specific chemical modifications on the ability of vaccines to produce splenomegaly in mice. | the surface of propionibacterium acnes, vpi 0009, was studied using microelectrophoresis following chemical treatments intended to modify specific charged groups. the effect of specific modifications on ability of cells to induce splenomegaly, an indicator of stimulation of the reticuloendothelial system, was also determined. there was little difference between ph mobility curves of p. acnes vpi 0009 and other strains of propionibacteria which were not able to stimulate the reticuloendothelial s ... | 1982 | 7093818 |
| quantitative studies of the secretion of complement component c3 by resident, elicited and activated macrophages. comparison with c2, c4 and lysosomal enzyme release. | to quantitate the secretion of complement component c3 by guinea pig peritoneal macrophages an enzyme-linked immunosorbent assay was developed. c3 secretion was studied in resident, elicited and activated macrophages and compared with release of hemolytically active c2 and c4, as well as the lysosomal enzyme beta-d-2-acetamido-2-deoxyglucosidase. resident macrophages secreted about 6 ng c3/10(6) cells/h into culture supernatants over a period of 12 h. corynebacterium parvum-activated cells were ... | 1982 | 7094993 |
| effect of diving and diving hoods on the bacterial flora of the external ear canal and skin. | the bacterial flora of the external ear canals and posterior auricular skin surfaces were investigated in a group of 26 divers after 25 dry-suit dives in harbor water and 20 dry-suit dives in clear test tank test. a control group of 16 divers wore rubber hoods 19 times for a similar period (25 to 30 min) but did not dive. the protective effect of 2% acetic acid was tested by instilling it in the left ear of 14 divers and 8 nondivers. staphylococcus epidermidis, propionibacterium acnes, alpha-hem ... | 1982 | 7096559 |
| recovery of microorganisms from synovial and pleural fluids of animals using hyperosmolar media. | l-phase (cwd) broth and plate media were used in parallel with conventional microbiological media during a 3-year period for culturing synovial and pleural fluids of animals. two kinds of recoveries were obtained where parallel conventional methods were negative: (1) parent or normal bacteria, in very low numbers; and (2) type b cwd variants in equally low numbers. organisms in group 1 were: streptococcus zooepidemicus from horses (2x); beta-hemolytic streptococci, lancefield gp. g (2x); staphyl ... | 1982 | 7101719 |
| corynebacterium parvum prevents immunization to admixed irradiated tumor cells by a local process. | immunization of mice with 10(6) irradiated lstra murine leukemia cells (lx) induced weak immunity to subsequent tumor-cell challenge. admixture of low doses (1.4-14 micrograms of c. parvum with the lx usually augmented the immunity. higher doses (1,400-7,000 micrograms) of admixed c. parvum not only failed to augment immunity, but prevented immunization by the contained lx. we investigated the mechanism by which 1,400 micrograms of c. parvum mixed with 10(6) lx prevents immunization by the lx. t ... | 1982 | 7107070 |
| effects of diphenylhydantoin on killer cell activity and other immunological functions. a sequential study including the interaction of corynebacterium parvum in melanoma patients. | the effects of a single phenytoin dose, given to patients with malignant melanoma, upon peripheral blood counts, serum immunoglobulins, lymphocyte subpopulations and lymphocytotoxicity (using chang target cells) were recorded. sequential blood samples were taken before and 10, 14, 34, 38 and 58 h after the phenytoin. early reductions (p less than 0.05) in lymphocyte count, nk, k and pha induced cytotoxicity, when compared with initial, pre-phenytoin values were noted. immunisation with i.v. c. p ... | 1982 | 7107099 |
| a randomized study of combination chemotherapy (vac-fmc) with or without immunostimulation by corynebacterium parvum in metastatic breast cancer. | a total of 156 patients with metastatic breast cancer were entered into a prospective multi-center trial in september 1975. all patients were treated monthly with vincristine, adriamycin and cyclophosphamide (vac) six times, followed by 5-fluorouracil, methotrexate and cyclophosphamide (fmc) until progression was documented. by random assignment, the patients received 5 mg/m2 corynebacterium parvum (cp) subcutaneously on day 1, in addition to vac/fmc. of the 150 evaluable patients, 33 of 76 (45% ... | 1982 | 7109499 |
| a simplified medium for detecting the effect of lecithin on the growth of propionibacterium acnes. | thirty strains of propionibacterium acnes were assayed to detect the effect of lecithin on their growth and to test the nutritional requirement of this bacterium for this phospholipid. the liquid lecithin medium (llm) and solid lecithin medium (slm), containing purified bovine lecithin (pbl) supported the growth of p. acnes strains assayed. two percent pbl, which was an optimal concentration of lecithin (w/v in llm and slm) for the normal growth of p. acnes, was found to be inhibitory for the gr ... | 1982 | 7109967 |
| splenic abscess caused by propionibacterium acnes. | a 59-year-old male diabetic was admitted with an acute myocardial infarction and had recurrent. propionibacterium acnes bacteremia. fifteen months after the initial admission a splenectomy was required for removal of a large splenic abscess caused by p. acnes. although this organism represents part of the normal skin flora, its presence of blood cultures requires serious evaluation since it may signify clinical disease, not merely contamination of blood cultures by skin flora. | 1982 | 7113264 |
| synergism of the antimicrobial agents miconazole and benzoylperoxide. | | 1982 | 7115614 |
| the natural history of resectable metastatic melanoma (stage iva melanoma). | one-hundred-two patients with malignant melanoma who had distant metastases surgically resected and were judged to be clinically free of disease (m. d. anderson stage iva melanoma) were studied. the median survival for all the patients from time of diagnosis of stage iva disease was 18 months. the site of the resected metastases did not appear to influence survival, being approximately the same for the brain (15 months), lung (16 months), intraabdominal (18 months), and skin and/or lymph nodes ( ... | 1982 | 7116298 |
| role of corynebacterium parvum in the activation of peritoneal macrophages. 1. association between intracellular c. parvum and cytotoxic macrophages. | | 1982 | 7116464 |
| septic arthritis caused by propionibacterium acnes. | the traditional view that propionibacterium acnes is nonpathogenic for man, except as an agent associated with acne vulgaris, has been refuted. propionibacterium acnes has been shown to cause endocarditis, meningitis, and other serious infections. we report a case of p acnes infection causing septic arthritis and contributing to a concurrent soft-tissue infection. this case supports and extends previous observations of the clinical importance of p acnes as a pathogen. a positive culture for p ac ... | 1982 | 7120595 |
| evaluation of a metronidazole disk test for the presumptive identification of anaerobes. | a total of 632 bacterial strains recovered under anaerobic conditions from clinical specimens were tested from their susceptibility to metronidazole by a disk diffusion test using 5 micrograms metronidazole disks. three-hundred-fifty-five of the 632 bacterial strains exhibited susceptibility the metronidazole, and each was determined to be an obligate anaerobe. the remaining 277 isolates showed resistance to the 5 micrograms disk. of these resistant strains, 257 were determined to be facultative ... | 1982 | 7124785 |
| effect of ph on growth rates of rumen amylolytic and lactilytic bacteria. | the relationship between the ph of the medium and specific growth rates, in well-buffered media at 38.5 degrees c, was determined for three strains of butyrivibrio fibrisolvens and for one strain each of streptococcus bovis, selenomonas ruminantium subsp. lactilytica. megasphaera elsdenii, veillonella alcalescens, and propionibacterium acnes. the ph optima for growth were between 6.1 and 6.6 for all six species, and the upper ph limits were between 7.3 and 7.8. the lower limit ph values for grow ... | 1982 | 7125656 |
| effect of c. parvum on the number and activity of macrophages in primary and transplanted murine fibrosarcomas. | | 1982 | 7126431 |
| corynebacterium parvum antibodies in healthy and cancer patients. | | 1982 | 7129515 |
| a comparison of saponin with other adjuvants for the potentiation of protective immunity by a killed plasmodium yoelii vaccine in the mouse. | the protective immunity conferred by subcutaneous injection of outbred cd-1 mice with a killed plasmodium yoelii (ym strain) vaccine was strongly potentiated by saponin. by adjusting the dose of antigen, the number of immunizations and the number of living parasites in the challenge infection, conditions were defined where antigen alone was non-protective but 100% protection was obtained by the addition of saponin. inbred balb/c, cba/ca and c57 b1 mice were much less responsive than the cd-1 mic ... | 1982 | 7145465 |
| [immunopotentiation in the treatment of malignant diseases. cellular immunity in patients with malignoma of the lung treated with corynebacterium parvum]. | | 1982 | 7147792 |
| morphology of draining lymph nodes after local immune stimulation with c. parvum: comparison of pelvic nodes in carcinoma of cervix and popliteal and inguinal nodes of guinea-pig. | morphological changes are described in pelvic lymph nodes excised 10 days after c. parvum (cp) treatment of patients with cervical carcinoma. guinea-pig popliteal and inguinal lymph nodes were investigated from days 1 to 10 after an injection of 70 micrograms cp into the footpad. eosinophils were detected from the first few hours after stimulation, initially in the marginal sinus, then in the medullary sinuses and subsequently in the efferent lymphatics. from day 2 to day 6, histiocyte accumulat ... | 1982 | 7150473 |
| serum biochemical changes in c. parvum-injected mice bearing the landschütz ascites carcinoma. | mice injected i.p. with 1.4 mg corynebacterium parvum (c. parvum) displayed minor transient impairment of hepatic function, as evidenced within 5-8 days by significant elevations in serum aspartate aminotransferase and reductions in total protein, albumin and alkaline phosphatase. inoculation of animals with the landschütz ascites carcinoma (lac) caused similar but more marked and prolonged liver injury with serological evidence of nephrotoxicity at advanced stages of tumour growth. injection of ... | 1982 | 7150511 |
| macrophage functional heterogeneity. | | 1982 | 7158494 |
| influence of macrophage activation on the synthesis of complement components c2, c3, c4. | | 1982 | 7158498 |
| the effect of c. parvum therapy on intratumoral macrophage subpopulations. | | 1982 | 7158508 |
| have extemporaneously compounded topical antibiotics a place? the clindamycin saga. | | 1982 | 7161090 |
| a note on a selective medium for the isolation of cutaneous propionibacteria. | | 1982 | 7161221 |
| a study of anaerobic bacteria in meibomian abscess. | | 1982 | 7161584 |
| effect of corynebacterium parvum on bone marrow macrophage colony production, peripheral blood leukocytes, and histologic changes of tissues in calves. | an iv injection of formalin-killed corynebacterium parvum in calves did not cause a marked increase in the number of bone marrow macrophage colonies or peripheral blood monocytes, granulocytes, or lymphocytes. in treated calves, the spleen increased in weight and there were macrophage infiltrations in liver, lungs, spleen, and lymph nodes. lymphoid hyperplasia was observed in bronchial-associated lymphoid tissue, cortical areas of the thymus, and in primary and secondary follicles and the paraco ... | 1982 | 7165173 |
| corynebacterium parvum: antibodies measured with the enzyme-linked immunosorbent assay (elisa) in normal human sera and sera from cancer patients undergoing treatment. | an enzyme-linked immunosorbent assay (elisa) was developed to monitor the antibody response to corynebacterium parvum during cancer immunotherapy. firstly, in sera from 176 clinically healthy individuals not treated with c. parvum, elevated elisa extinction values were observed from the age of 11 years onwards for both sexes; these high values were probably due to preexisting antibodies to c. parvum or related organisms. consequently, in order to evaluate c. parvum antibody levels after treatmen ... | 1982 | 7165281 |
| the effect of intravenous corynebacterium parvum on gut associated mononuclear phagocytes in normal and tumour bearing rats. | corynebacterium parvum, which has been used in the treatment of human colorectal cancer, probably exerts its action through cells of the mononuclear phagocyte system (mps). in this study the effect of systemically administered c. parvum has been measured on gut associated mps cells in normal and colorectal cancer bearing rats. mps cells are not normally found in samples of lymph obtained after cannulation of the thoracic duct (tdc). however, after total extirpation of the mesenteric lymph nodes, ... | 1982 | 7165995 |
| nonspecific cytotoxic cells induced in vivo and their comparison to culture-induced cytotoxic cells. | nonspecific cytotoxic cells in peritoneal exudate cells obtained from mice injected with p. acnes and other stimulants were characterized using mm2 ascites mammary carcinoma as target cells which were insensitive to lytic activity of natural killer cells and of activated macrophages. mm2-lytic activity was recovered in nonadherent cells after incubation on glass surface for 1 hr, although it was recovered only partly in nonadherent fraction after extensive adherence. the characteristics of the r ... | 1982 | 7169668 |
| exoenzymes of propionibacterium acnes. | thirty strains of propionibacterium acnes were grown in basal salt medium containing lecithin as a lipid substrate and in other media. the cultures were assayed for production of lipase (measured as fatty acid esterase) and other exoenzymes. lipase was assayed spectrophotometrically; other enzymes were assayed using the api zym system (analytab products inc., plainview, ny). substance for lipase were alpha- and beta-naphthol esters of propionic, butyric, valeric, caprylic, lauric, myristic, and ... | 1982 | 7172137 |
| [experimental studies on the immunomodulation of propionibacterium acnes]. | | 1982 | 7181661 |
| [stimulating immunity with the use of corynebacterium parvum after resection of carcinoma of bronchial origin. clinical aspects]. | | 1982 | 7182936 |
| [antitumor effect of corynebacterium parvum on c3h mouse bearing mh-134 tumor]. | the antitumor effect of corynebacterium parvum (c. parvum) was examined by using c3h/he mouse bearing mh-134 ascites hepatoma. the optimal dose of c. parvum was 0.4mg/day intraperitoneally for 5 consecutive days from the 5th day after tumor inoculation. the cell-mediated immunity in this treatment was also investigated by delayed type hypersensitivity (dth) and macrophage migration inhibition activity (mi). according to the results of both dth and mi, it was assumed that c. parvum yielded an ant ... | 1982 | 7184438 |
| studies on antitumor effect of corynebacterium parvum and pustulan on lewis lung carcinoma in mice. | antitumor effect of two biological adjuvants and their supported mechanism was studied in mice inoculated s.c. with lewis lung carcinoma (ll) cells. the studies included in vivo tests: median survival time (mst) evaluation, tumor weight inhibition (twi) and splenohepatomegaly measurements. the nonspecific in vitro cytostatic effect of peritoneal adherent cells stimulated with adjuvants was measured by inhibition of 3h thymidine incorporation into tumor cells. in in vivo studies the reproducible ... | 1982 | 7184489 |
| [pathogenic role and antibiotic sensitivity of propionibacterium acnes]. | | 1982 | 7187208 |
| requirements for successful immunotherapy and chemoimmunotherapy of a murine model of ovarian cancer. | a comprehensive study of nonspecific immunotherapy has been conducted in an established murine model of ovarian cancer in order to determine the relative effectiveness of commonly used bacterial immunostimulants, the importance of the route and schedule of administration of these agents, and their effects in combination with chemotherapy. implants of 10(5) or 10(6) ovarian tumor cells i.p. kill all syngeneic c3heb/fej mice within 25 days. corynebacterium parvum (700 microgram/mouse i.p. 24 hr af ... | 1981 | 7193085 |
| doxorubicin, cisplatin, and corynebacterium parvum in non-small cell bronchogenic carcinoma. | forty previously untreated patients with stage iii non-small cell bronchogenic carcinoma were treated with doxorubicin (adriamycin) at a dose of 60 mg/m2 iv on day 1 and cisplatin (cis-diamminedichloroplatinum[ii]) at a dose of 60-80 mg/m2 iv on day 1, every 4 weeks. each patient was randomized to receive iv corynebacterium parvum either on day 1 or on day 14 of each 4-week cycle. there were no complete responses and only seven of 40 (17.5%) patients achieved a partial response, with an overall ... | 1980 | 7193520 |
| studies on pathogenicity and persistence of propionibacterium acnes in mice. | the pathogenicity of propionibacterium acnes was studied using immunodeficient and germ-free (gf) mice. p. acnes injected i.p. to immunocompetent (ddy and balb/c-nu/+) and -deficient mice (cba/n and balb/c-nu/nu) decreased rapidly and only as small number were recovered from organs from day 7 on. the organisms injected similarly into gf-immunocompetent (gf-cf1) mice also decreased but when injected into gf-immunodeficient (gf-balb/c-nu/nu) mice a small number persisted as late as week 4. when gf ... | 1981 | 7200303 |
| rapid temperature programmed gas-liquid chromatography of volatile fatty acids (c1-c7) for the identification of anaerobic bacteria. | a gas liquid chromatography method for the separation of 10 volatile fatty acids (c1-c7 and isomers) has been improved by using oven temperature programmed conditions. in our conditions, the proprietary stationary phase sp 1220 introduced by supelco inc., gave sharp separation of volatile fatty acids in less than 8 min. this method was suitable for analyses with both thermal conductivity and flame ionization detectors. | 1980 | 7202642 |
| immunomodulation of chemically induced fibrosarcoma by corynebacterium parvum. | | 1980 | 7203570 |
| propionibacterium acnes infection following subdural tap. | the case of an infant who had subdural hematomas that became infected with propionibacterium acnes is reported. this is the second reported case of intracranial propionibacterium acnes infection resulting from diagnostic or therapeutic manipulation. | 1981 | 7211765 |
| intrapleural immunotherapy with corynebacterium parvum in recurrent malignant pleural effusions. | twenty-one patients with proven recurrent malignant pleural effusions were randomly allocated to treatment groups receiving either intrapleural corynebacterium parvum in a dose of 7 mg or intrapleural mustine (20 mg). the designated intrapleural therapy was repeated on one occasion if further pleural aspiration was required. corynebacterium parvum (nine patients) proved superior to mustine (12 patients) in suppressing the reaccumulation of pleural fluid, and was associated with only minimal side ... | 1980 | 7221983 |
| serum zinc levels in lung cancer patients. | serum zinc concentrations were determined in 26 extensive squamous cell lung cancer patients and were tested for correlations with survival, response to therapy, nutritional status indices, and various host defense characteristics. subnormal serum zinc levels were found in 24 of the 26 patients. the mean serum zinc concentration was 43.2/micrograms 100 ml +/- 3.6 s.e.m. (normal = 80-100 micrograms/100 ml). a significant (p = 0.007) survival advantage was demonstrated for those patients with pret ... | 1981 | 7226079 |
| potentiation of macrophage tumor cytostasis by tumor-induced ascites. | | 1981 | 7229369 |
| prostaglandin formation in bacteria : a reevaluation. | we report here that production and release of pge2 do not occur in common bacteria. the apparent production in the presence of arachidonic acid, previously reported (1) may be explained by pge2 contamination and autooxidation of the aa used. the presence of pge2 like material in some but not all isolates of propionibacterium acnes is confirmed. | 1981 | 7232767 |
| some "pharmacologic' data about corynebacterium parvum. | | 1980 | 7232843 |
| propionibacterium acnes central nervous system shunt infection. commercial blood culture medium-dependent isolation of the bacterium. | a 58-year-old woman had a central nervous system shunt infection and septicemia caused by propionibacterium acnes. during a two-year period, many becton-dickinson blood cultures (18 of 39) were positive for p. acnes, but all bactec blood cultures (15) were negative. parallel cultures in the two commercial media performed simultaneously on the same blood samples several times resulted in a positive becton-dickinson culture and a negative bactec culture. this case reemphasizes that some bacterial ... | 1981 | 7234759 |
| effects of the landschütz ascites carcinoma and ascitic fluid on macrophage activity in c. parvum-injected mice. | i.p. administration of 1.4 mg corynebacterium parvum (c. parvum) 24 h before inoculation of landschütz ascites carcinoma (lac) cells significantly impaired growth of the tumour in mf1 mice. the injection of tumour cells caused a transient inhibition of the activity of the mononuclear phagocyte system (mps) in both normal and c. parvum-treated hosts, as evidenced by impaired clearance of colloidal carbon from the bloodstream and reduction in hepatic phagocytosis of 51cr-labelled sheep erythrocyte ... | 1981 | 7236491 |
| coumarin or warfarin treatment of mice does not increase the microbicidal or tumoricidal capacities of macrophages. | benzopyrones have been shown to affect several functions of macrophages. we examined the effects of two benzopyrones, coumarin and warfarin, on the capacity of mouse macrophages to inhibit microorganisms and tumour target cells. mice were treated with daily i.v. doses of either drug. then the mice were challenged with lethal doses of toxoplasma gondii or peritoneal macrophages from these mice were challenged in vitro with t. gondii or tumour target cells survival of coumarin or warfarin-treated ... | 1981 | 7236495 |
| augmentation of natural killer cell activity in spleens of infant, aged, and low responder strain mice by corynebacterium parvum. | murine natural killer (nk) cell activity is both age- and strain-dependent. nk activity does not appear in murine spleen cells until three weeks after birth. activity peaks at approximately 10 weeks, decreasing thereafter with mice over one year old showing significantly reduced levels. mice showing low or no nk activity because of age (aged and infant mice, respectively) can be stimulated to show significant levels of nk lysis by i.p. injection of formalin killed corynebacterium parvum (cp). in ... | 1981 | 7238649 |
| prostaglandin-like substances in propionibacterium acnes. v. activity profiles using cascade superfusion bioassay and platelet aggregation. | | 1981 | 7238744 |
| comparison of cytotoxic and microbicidal function of bronchoalveolar and peritoneal macrophages. | studies were carried out with mice to explore in vitro the effector function(s) of macrophages from two different anatomical compartments (peritoneal cavity and lungs). the cytotoxic capacity of macrophages was measured by determining their cytostatic and cytocidal effects on el-4 tumour target cells, and the microbicidal capacity of macrophages was measured by determining their ability to kill or inhibit the intracellular protozoan, toxoplasma gondii. neither peritoneal macrophages (pm) nor bro ... | 1981 | 7239556 |
| preventive effect of propionibacterium acnes on metastasis in mice rendered tolerant to tumor-associated transplantation antigens. | the present study was carried out to investigate the preventive effect of propionibacterium acnes (p. acnes) on metastasis formation of c3h/he mice which has been rendered tolerant to tumor-associated transplantation antigen (tata) of syngeneic x5563 plasmacytoma. c3h/he mice inoculated intravenously (iv) with 10(6) 7000 r x-irradiated x5563 tumor cells 3 times at 4-day intervals. these pretreated mice could not develop immune resistance against x5563 tumor even after the appropriate immunizatio ... | 1980 | 7248045 |
| corynebacterium parvum treatment of p388 tumor-bearing mice. i. lysosomal enzyme levels in adherent peritoneal cells and peritoneal lavage fluid. | bdf1 mice treated with corynebacterium parvum (c. parvum) 2 days before an implant of 106 p388 leukemic cells had up to an 110% increase in survival time above control; bacillus calmette-guérin (bcg) treatment was ineffective. acid phosphatase and beta-glucuronidase were measured in adherent peritoneal lavage cells and beta-glucuronidase in peritoneal lavage fluid form mice treated with c. parvum or bcg 2 days before the implant of p388 cells. in the presence of the tumor, adherent peritoneal ce ... | 1981 | 7252179 |
| corynebacterium parvum treatment of p388 tumor-bearing mice. ii. lysosomal enzyme levels associated with p388 tumor cells. | the number of tumor cells recovered form the peritoneal cavity of mice administered corynebacterium parvum (c. parvum), bacillus calmette-guérin (bcg) or saline 2 days before tumor implant was assessed on days 1, 2, 3, 5 and 7 following the intraperitoneal (ip) administration of 10(6) p388 leukemic cells. c. parvum-treated mice manifested a significant decrease in the number of tumor cells recovered from the peritoneal cavity on days 3-7, while bcg-treated mice had tumor cell yields comparable t ... | 1981 | 7252180 |
| delayed hypersensitivity reactions vs chemotherapy and immunotherapy responses in women with ovarian adenocarcinoma. | fifty-one previously untreated women with figo stages iii and iv ovarian adenocarcinoma underwent sequential monthly skin testing utilizing keyhole limpet hemocyanian, varidase, mumps, monilia, and a purified protein derivative tuberculin (ppd) prior to each course of systemic chemotherapy (with or without corynebacterium parvum). the purpose of the study was to evaluate the patients' ability to demonstrate a delayed cutaneous hypersensitivity reaction to these sensitizing antigens and response ... | 1981 | 7253658 |
| influence of the "corynebacterium parvum" vaccine and stilbostat on intratesticular development of walker 256 carcinosarcoma in hyper- or hypothyroidized wistar rats. | this work studies the effect of nonspecific antitumoral immunity produced by the "corynebacterium parvum" vaccine (c.p.) and stilbostal on the development of walker 256 carcinosarcoma intratesticularly grafted to hyper- or hypothyroidized wistar rats. the thyroid hormone level was altered by administration of thyroxin, alkiron or surgical thyroidectomy. the c.p. vaccine was given in a mixture with tumoral cells suspension and the treatment with the stilbostat drug was started 5 days from graftin ... | 1981 | 7256164 |
| correlation of propionibacterium acnes populations with the presence of triglycerides on nonhuman skin. | the skins of mice, rats, rabbits, sheep, guinea pigs, and dogs were cultured for propionibacterium acnes. only the sebaceous regions (perianal gland) of guinea pigs harbored a significant p. acnes population. analysis of the lipid from this region revealed a significant percentage of triglycerides, compounds lacking in the sebum of the other animals. | 1981 | 7259157 |
| organ distributions and clearance studies of 99mtechnetium-labeled corynebacterium parvum in patients with leukemia. | the clearance, metabolism and localization of corynebacterium parvum (c. parvum) labeled with 99mtechnetium (99mtc) given intravenously was studied in various leukemia patients to develop the method and to evaluate reticuloendothelial systems (res) function. a computer program was utilized to characterize the clearance. c. parvum 0.05 mg was labeled aseptically with 10 mci of 99mtc with a reducing agent. it was injected intravenously over 5 s and clearance was characterized over the next 60 min. ... | 1981 | 7263121 |
| use of mini-microaggregated albumin to study reticuloendothelial system (res) function in c. parvum-treated animals. | mini-microaggregated albumin colloid (particle size less than 80 a) and microaggregated albumin colloid (particle size 500-1000 a) made from human serum albumin were conjugated with 99mtechnetium and used to study reticuloendothelial system (res) function in animals treated intravenously with c. parvum. the uptake in blood, liver and spleen were studied in untreated mice and in mice at various times after treatment with 150 or 300 micrograms of c. parvum. the blood clearance of these materials w ... | 1981 | 7263122 |
| chemoimmunotherapy (dtic and corynebacterium parvum) for disseminated malignant melanoma. | | 1981 | 7273032 |
| activation of the alternative pathway of complement in human serum by propionibacterium acnes (corynebacterium parvum) cell fractions. | activation of the alternative pathway of complement is known to be initiated by bacterial structures. we have fractionated propionibacterium acnes cells, purified various cell fractions, and tested their complement-activating ability in human serum chelated with ethyleneglycol bis-(beta-aminoethylether)-n,n1-tetraacetic acid. the majority of complement-activating activity was localized in the wall fraction. this activity was resistant to lipid extraction, protease, rnase, dnase and lysozyme trea ... | 1981 | 7275277 |
| regional variations in density of cutaneous propionibacteria: correlation of propionibacterium acnes populations with sebaceous secretion. | cutaneous propionibacterium acnes populations were quantitatively measured in 33 young adults and compared with the rate and composition of sebum secretion in nine skin regions. bacteriological and lipid analyses were performed on the forehead, cheek, anterior chest, abdomen, lower back, volar forearm, upper inner arm, thigh, and calf. p. acnes populations in these sites correlated significantly with the total amount of lipid produced (r = 0.77) as well as with di- and triglycerides (r = 0.68), ... | 1980 | 7276142 |
| immunity to transplantable nitrosourea-induced neurogenic tumors. i. potentiation of tumor immunity with corynebacterium parvum. | various injection schedules of c. parvum and tumor cells of the nitrosourea-induced malignant neurinoma, tr-481, were used to induce tumor immunity in syngeneic cdf rats. although subcutaneous injection of the poorly immunogenic tr-481 cells alone or with c. parvum caused retardation of growth of 2 x 10(5) tr-481 cells injected 1-3 weeks later, no significant difference in tumor size or incidence was obtained, as judged by tumor growth at 8 weeks. in contrast, injection of tr-481 with c. parvum ... | 1981 | 7276992 |
| dtic and actinomycin d with and without c. parvum immunotherapy in advanced malignant melanoma. | | 1981 | 7281240 |
| adriamycin-activated macrophages as tumor growth inhibitors. | flow cytometry has been used to study the influence of adriamycin- and corynebacterium parvum-elicited cytostatic macrophages on the cell cycle of hela cells. macrophages harvested from the peritoneal cavity of mice given i.p. injections of adriamycin retain cytostatic activity for 3 to 4 days in vivo. many of these macrophages fluoresced with the red emission spectra characteristic of adriamycin. the decline in the presence of macrophages exhibiting red fluorescent cytoplasmic particles paralle ... | 1981 | 7284993 |
| morphological and functional aspects of active specific immunotherapy of established pulmonary metastases in guinea pigs. | | 1981 | 7285011 |
| metabolic host reaction in response to the proliferation of nonmalignant cells versus malignant cells in vivo. | | 1981 | 7285018 |
| inhibitory effect of propionibacterium acnes-activated macrophages on the tumor metastasis enhanced by tumor-specific immunosuppression. | the present study deals with the mechanism by which propionibacterium acnes (p. acnes) prevents metastasis formation in c3h/he mice which had been rendered tolerant to tumor-associated transplantation antigen (tata) of syngeneic x5563 plasmacytoma by intravenous (iv) inoculation of 10(6) x-irradiated tumor cells 3 times at 4-day intervals. in these tata-tolerant mice, systemic metastasis formation was enhanced after intradermal (id) implantation of viable tumor cells, even if the tumor was resec ... | 1981 | 7286546 |
| the isolation of a lectin-like molecule from corynebacterium parvum (nctc 10390). | a mannose specific lectin has been isolated by affinity chromatography from the cell wall of c. parvum. polyacrylamide gel electrophoresis indicates that the lectin molecules lie in the molecular weight range 57,000--72,000. it appears that c. parvum like e. coli and salmonellae express lectins that bind to cells expressing mannose in their membranes. this may partly account for the interaction between c. parvum and the macrophage leading to the various immunological phenomena associated with c. ... | 1981 | 7288872 |