| antibodies to htlv-i p24 in african and portuguese populations. | using a radioimmunoassay to detect htlv-i protein antibodies of molecular weight 24,000, we screened populations from algeria (140 subjects), tunisia (442), mali (69), senegal (415), uganda (135), the central african republic (77), the congo (360), and madagascar (193). only four subjects were positive (1 from senegal, 1 from uganda, 2 from the congo). this is a much lower figure than that found by others in africa by the enzyme-linked immunosorbent assay technique. in addition, 319 portuguese b ... | 1985 | 2861895 |
| transformation of primary cultured and co-cultured adult rat liver cells by 3'-methyl-4-dimethylaminoazobenzene. | under various conditions of culture and carcinogen treatment, the transformation of liver cells by 3'-methyl-4-dimethylaminoazobenzene (3'-me-dab) was studied. primary liver cell (plc) cultures from adult male rats and co-cultures with plcs of arl-d8 cells of a liver epithelial-like clear cell line from adult female rats were treated with 0.24 mm 3'-me-dab for 6 days. four of 8 carcinogen-treated plc cultures contained cells with marker chromosomes, and 3 of the 8 cultures contained gamma-glutam ... | 1985 | 2862759 |
| [relationship between hosts and hemorrhagic fever renal syndrome in chang-an county, shan-xi province]. | | 1985 | 2862998 |
| characterization of enhanced intestinal permeability; electrophysiological study on the effects of diclofenac and ethylenediaminetetraacetic acid. | the mechanism of the enhancing effects of diclofenac sodium and disodium ethylenediaminetetraacetate (edta) on intestinal permeability to sulphanilic acid were investigated in the rat isolated jejunum by an electrophysiological technique. the results suggested that diclofenac sodium increased the mucosal-to-serosal flux rate of sulphanilic acid by enhancing both transcellular and paracellular permeabilities while the effect of edta was located only in the paracellular pathway. | 1985 | 2863363 |
| iga response during accidental infection with shigella flexneri. | | 1985 | 2863666 |
| combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis. | | 1985 | 2863679 |
| immunocytochemical studies of somatostatin neurons in brain. | immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. for example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. somatostatin was also the first n ... | 1985 | 2863929 |
| glial differentiation in dissociated cell cultures of neonatal rat brain: noncoordinate and density-dependent regulation of oligodendroglial enzymes. | the mixed glial system of primary cultures of cells dissociated from neonatal rat brain was utilized to study glial differentiation. the investigation was addressed specifically to the possibility of noncoordinate regulation of two manifestations of oligodendroglial differentiation, i.e., activities of glycerol-3-phosphate dehydrogenase (gpdh) and of 2',3'-cyclic nucleotide 3'-phosphohydrolase (cnp), as well as the effects of initial cell density on the time of onset and the intensity of express ... | 1986 | 2870195 |
| transglutaminase and neuronal differentiation. | during mouse brain maturation cellular transglutaminase specific activity increases 2.5 fold from day 3 to adulthood. a more pronounced increase is seen during morphological differentiation of mouse neuroblastoma cells, where serum withdrawal induces neurite outgrowth concomitant with a 10 fold increase in transglutaminase specific activity. in contrast, non-dividing neuroblastoma cells lacking neurites show only a 1.5 fold increase in enzyme specific activity. transglutaminase activity does not ... | 1986 | 2870428 |
| transglutaminase and neuronal differentiation. | during mouse brain maturation cellular transglutaminase specific activity increases 2.5 fold from day 3 to adulthood. a more pronounced increase is seen during morphological differentiation of mouse neuroblastoma cells, where serum withdrawal induces neurite outgrowth concomitant with a 10 fold increase in transglutaminase specific activity. in contrast, non-dividing neuroblastoma cells lacking neurites show only a 1.5 fold increase in enzyme specific activity. transglutaminase activity does not ... | 1986 | 2870428 |
| integrating treatments in schizophrenia. | schizophrenia is a common and severe illness found throughout the world. onset is usually in adolescence and young adulthood, and many individuals have illness-related impairments for life. schizophrenia assaults the very essence of what is distinctly human. the personality is devastated, subjective experience and thought distorted, and the psychological building blocks of everyday life torn asunder. this article discusses diagnostic and conceptual considerations, pharmacotherapy, and interperso ... | 1986 | 2870478 |
| influence of dietary lipids on the effect of chlorpromazine on membrane properties of rabbit red cells. | the effect of diets containing different types of common natural oils on physical properties of red cells was investigated by using rabbits. the rabbits were fed for 18 months on a standard diet in which 8% of its energy content was provided by safflower oil and 32% energy by either more safflower oil or fish oil, linseed oil, olive oil or palm oil. erythrocyte deformability was significantly decreased by the fish oil diet compared with each of the other diets. osmotic fragility was significantl ... | 1986 | 2870499 |
| platelet aggregation caused by carybdea rastonii toxins (crtx-i, ii and iii) obtained from a jellyfish, carybdea rastonii. | the pharmacological mechanisms of platelet aggregation induced by highly toxic proteins (crtx-i, crtx-ii, and crtx-iii) obtained from tentacles of a jellyfish, carybdea rastonii, were investigated. when the partially purified toxin (pcrtx) and crtxs were added to the citrated platelet-rich plasma (prp), aggregation was produced in a concentration-dependent manner. the activity of crtxs was approximately 100 times more potent than pcrtx. the crtxs-induced aggregation was little affected by indome ... | 1986 | 2870502 |
| blood-finding strategy of a capillary-feeding sandfly, lutzomyia longipalpis. | salivary gland homogenates of adult female lutzomyia longipalpis inhibited platelet aggregation induced by adp and collagen. apyrase (atp diphosphohydrolase) activity was prominent, requiring ca2+ but not mg2+ and a ph optimum of 8.0. human as well as rabbit hosts developed a well delimited erythema, evident 2-3 min after initial probing and lasting for as long as 2 days. erythema, not accompanied by itching or swelling, developed in previously exposed hosts as well as in those not previously ex ... | 1986 | 2870860 |
| blood-finding strategy of a capillary-feeding sandfly, lutzomyia longipalpis. | salivary gland homogenates of adult female lutzomyia longipalpis inhibited platelet aggregation induced by adp and collagen. apyrase (atp diphosphohydrolase) activity was prominent, requiring ca2+ but not mg2+ and a ph optimum of 8.0. human as well as rabbit hosts developed a well delimited erythema, evident 2-3 min after initial probing and lasting for as long as 2 days. erythema, not accompanied by itching or swelling, developed in previously exposed hosts as well as in those not previously ex ... | 1986 | 2870860 |
| pharmacokinetics of t-2 toxin and its metabolite ht-2 toxin, after intravenous administration in dogs. | the pharmacokinetics of t-2 toxin and ht-2 toxin were investigated comparatively in five dogs, after iv administration of the toxins (0.4 mg/kg). t-2 toxin was very rapidly and almost completely biotransformed to ht-2 toxin (fm = 83.6 +/- 3.9%). the following mean pharmacokinetic parameters were determined in this study for t-2 toxin and ht-2 toxin, respectively: half-life 5.3 +/- 2.1 and 19.6 +/- 4.7 min, clearance 0.107 +/- 0.056 and 0.167 +/- 0.074 liters/min/kg, and volume of distribution 0. ... | 1986 | 2870902 |
| pharmacokinetics of t-2 toxin and its metabolite ht-2 toxin, after intravenous administration in dogs. | the pharmacokinetics of t-2 toxin and ht-2 toxin were investigated comparatively in five dogs, after iv administration of the toxins (0.4 mg/kg). t-2 toxin was very rapidly and almost completely biotransformed to ht-2 toxin (fm = 83.6 +/- 3.9%). the following mean pharmacokinetic parameters were determined in this study for t-2 toxin and ht-2 toxin, respectively: half-life 5.3 +/- 2.1 and 19.6 +/- 4.7 min, clearance 0.107 +/- 0.056 and 0.167 +/- 0.074 liters/min/kg, and volume of distribution 0. ... | 1986 | 2870902 |
| two bradykinin binding sites with picomolar affinities. | bradykinin (bk) and related peptides exert a wide range of effects on several organ systems. we have attempted to sort out these effects by studying the binding interaction of [3h]bk at the membrane level with in vitro receptor binding techniques. high specific activity [3h]bk and an enzyme inhibitor "cocktail" has enabled us to label two bk binding sites with different affinity and peptide specificity in several guinea-pig tissues. in the guinea-pig ileum the high-affinity site has an equilibri ... | 1986 | 2871174 |
| potent antagonism of escherichia coli, bacteroides ovatus, fusobacterium varium, and enterococcus faecalis, alone or in combination, for enteropathogens in anaerobic continuous flow cultures. | interactions between representative strains of four predominant resident bacteria of the human colon, escherichia coli, enterococcus faecalis, bacteroides ovatus, and fusobacterium varium, and strains of seven enteropathogens, yersinia enterocolitica, shigella flexneri, salmonella typhimurium, vibrio parahaemolyticus, v. cholerae serogroup non o1, staphylococcus aureus, and clostridium perfringens, were examined in studies with an anaerobic continuous flow culture system and medium resembling th ... | 1986 | 2875188 |
| the neurobiologic consequences of down syndrome. | trisomy of the whole or distal part of human chromosome 21 (hsa 21) (ts21) results in down syndrome (ds), which is characterized in part by mental retardation and associated neurological abnormalities. structural abnormalities observed frequently include reduced brain weight, decreased number and depth of sulci in the cerebral cortices, neuronal heterotopias, and reduced numbers of specific populations of neurons, such as granule cells, in the cerebral cortices. abnormalities in the structure of ... | 1986 | 2875770 |
| the fine structure of atypical ciliated cells in the human gastric epithelium. | gastric mucosa obtained from the body and pyloric portions of the human stomach were observed by light and transmission electron microscopy. ciliated cells were found in two of 18 subjects examined, one patient with gastric ulcer and the other one with gastric adenocarcinoma. the ciliated cells were found in epithelia at sites away from the main lesions. the tissues containing ciliated cells showed intestinal metaplasia combined with mild chronic gastritis in both cases. the epithelial layer fac ... | 1986 | 2876543 |
| evidence for a migratory capability of rat kupffer cells to portal tracts and hepatic lymph nodes. | the present study concerns the migratory ability of kupffer cells in the rat. phagocytic cells were labeled with colloidal carbon or gold, these markers being administered intravenously either into a tail vein, which resulted in generalized reticuloendothelial uptake, or in low dose into the portal vein, which produced uptake by kupffer cells alone. cells containing marker were observed in the portal tracts and in hepatic lymph nodes from 1 to 3 days after injection into the portal vein. the dir ... | 1986 | 2876547 |
| 6,7-dichloro-1-(3,4,5-trimethoxybenzyl)-1,2,3,4-tetrahydroisoquinoline . a structurally novel beta-adrenergic receptor blocking agent. | replacement of the catecholic hydroxyl groups of the beta-adrenergic receptor agonist 6,7-dihydroxy-1-(3,4,5-trimethoxybenzyl)-1,2,3,4-tetrahydroisoquinoline (trimetoquinol) with chloro substituents results in a compound with marked beta-adrenoceptor antagonist properties. this, therefore, parallels the similar transformation of the beta-adrenoreceptor agonist isoproterenol into the antagonist dichloroisoproterenol. in a test for inhibition of isoproterenol-induced enhancement of the rate of con ... | 1986 | 2878080 |
| phenotypic modification of human glioma and non-small cell lung carcinoma by glucocorticoids and other agents. | glucocorticoids are cytostatic for human glioma grown at a high cell density in cell culture. the effect is not cytotoxic, appears to involve a modification of the cell surface, and has been detected with methyl prednisolone, dexamethasone, and beta-methasone. glucocorticoids were also found to reduce malignancy-associated properties (plasminogen activator and endothelial mitogenesis) and enhance differentiation (glutamyl synthetase activity and high affinity gaba uptake). cytostasis was also se ... | 1986 | 2879508 |
| plasma levels of gastrin, somatostatin, and cholecystokinin immunoreactivity during pregnancy and lactation in dogs. | plasma levels of gastrinlike, somatostatinlike, and cholecystokininlike immunoreactivity were determined by radioimmunoassay in peripheral blood from 11 beagles during pregnancy and in response to suckling during lactation. cholecystokinin was determined in pooled and in individual plasma samples after separation of cholecystokinin and gastrin by high-performance liquid chromatography. gastrin and somatostatin levels were significantly increased during the 8-wk-long dog pregnancy; peak values we ... | 1987 | 2880780 |
| media for isolating shigella. | | 1987 | 2881104 |
| differences in adhesiveness among type 1 fimbriate strains of enterobacteriaceae revealed by an in vitro hep2 cell adhesion model. | ten type 1 fimbriate strains of enterobacteriaceae were examined in an in vitro adhesion assay with hep2 epithelial cells. the range of hep2 cell adhesiveness, which was characteristic for each strain, was affected by motility, type 1 fimbriation and production of mannose sensitive haemagglutinin. nevertheless, not all type 1 fimbriate strains adhered well in this model. the findings are discussed with regard to the possibility that different type 1 fimbriate enterobacteria, though all are manno ... | 1986 | 2881915 |
| bw a575c, a chemically novel agent with angiotensin converting enzyme inhibitor and beta-adrenoceptor-blocking properties. | bw a575c (n-(1-(s)-carboxy-5-[4(3-isopropylamino-2-(r, s)-hydroxypropoxy)indole-2- carboxamido]pentyl)-(r, s)-alanyl-(s)-proline) is a chemically novel agent which exhibits in a single molecule both angiotensin converting enzyme (ace) inhibition and beta-adrenoceptor-blocking properties. bw a575c produced a competitive blockade of heart rate responses to isoprenaline in a guinea-pig right atrial preparation (pkb 7.18 +/- 0.05, cf. pindolol 8.9 +/- 0.7). bw a575c inhibited a partially purified pr ... | 1987 | 2882805 |
| glutathione metabolizing enzyme activities in human thyroid. | glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ... | 1987 | 2883074 |
| a competitive receptor binding radioassay for beta-1 and beta-2 adrenergic agents. | a rapid and sensitive competitive receptor binding assay for beta-1 and beta-2 adrenergic binding for adrenergic agents has been developed. the steps that are critical for the success of the assay are given in detail so that the assay can be set up in any routine laboratory with relative ease. the rationale behind the use of specific reagents is discussed. the assay requires microgram quantities of test compound, a radiolabeled specific beta adrenergic antagonist [3h]dihydroalprenolol (dha), and ... | 1987 | 2883662 |
| anti-surface antibodies to entamoeba histolytica and their role in complement lysis. | | 1986 | 2884957 |
| beta-blockade reverses regional dysfunction in ischemic myocardium. | to determine the protective effect of oxprenolol-induced beta-blockade on the compromised myocardium (critical constriction of the left anterior descending coronary artery) against the adverse effect of high concentrations of halothane, halothane dose-response curves were obtained in six dogs in each of three phases: preconstriction (control), critical constriction, and critical constriction with the addition of 0.3 mg/kg intravenous oxprenolol. the extent of depression of ventricular function w ... | 1987 | 2886076 |
| composite effects of actinonin when inhibiting enkephalin-degrading enzymes. | actinonin which has been found to be an inhibitor of aminopeptidase m (ec 3.4.11.2) also inhibited enkephalinase a (ec 3.4.24.11) and enkephalin aminopeptidase which were partially purified from the corpus striatum membrane of guinea-pig brain. the ic50 values were 5.6 microm for enkephalinase a and 0.39 microm for enkephalin aminopeptidase. actinonin also inhibited with an ic50 value of 1.1 microm dipeptidyl aminopeptidase tested on whole brain homogenate of rats in the presence of thiorphan an ... | 1987 | 2886348 |
| azodisalicylate (azodisal sodium) causes intestinal secretion. comparative study of the effect of azodisalicylate, sulfasalazine, 5-aminosalicylic acid and sulfapyridine on the water and electrolyte transfer and the morphology of the rat ileum and colon in vivo. | azodisalicylate (ads) is one of the possible successors of sulfasalazine in the treatment of ulcerative colitis. the following results were obtained when comparing the influence of ads on net water and electrolyte transfer in tied-off loops of the rat ileum and colon in vivo with sulfsalazine, 5-aminosalicylic acid, and sulfapyridine. (1) sulfasalazine, 5-aminosalicylic acid and sulfapyridine had no effect on water and electrolyte transfer of the healthy intestinal mucosa in concentrations up to ... | 1987 | 2887473 |
| activation of a common effector system by different brain neurotransmitter receptors in xenopus oocytes. | xenopus oocytes possess 'native' muscarinic receptors, which give rise to oscillatory chloride currents; similar responses are elicited by activation of foreign receptors to serotonin, glutamate and noradrenaline, expressed in oocytes after injection of messenger rna from rat brain. when low concentrations of two agonists are applied together, the combined response is greater than would be expected from the sum of the responses to each agonist applied alone. potentiation of acetylcholine by sero ... | 1987 | 2888117 |
| bacterial diarrhoea and treatment. | | 1988 | 2893218 |
| sex as a risk factor for htlv-i spread among intravenous drug abusers. | | 1988 | 2895250 |
| enhancement of contractile responses to partial alpha-adrenoceptor agonists during warming in rat aorta. | we examined the effects of warming on the contractile responses to full and partial alpha-adrenoceptor agonists in rat aorta. the contractions elicited by norepinephrine and methoxamine were not affected during warming (40 degrees c, 42 degrees c), whereas those induced by clonidine and st 587 were significantly enhanced. kcl-induced contractions of rat aorta were not affected by warming. the dissociation constants of clonidine and st 587 at 40 degrees c were not different from those at 37 degre ... | 1987 | 2895427 |
| direct effects of alpha 2-adrenergic receptor stimulation on intravascular systemic capacity in the dog. | the role of alpha 2-adrenergic receptor stimulation in the regulation of systemic vascular capacity and venous return, a major determinant of cardiac output, is not well understood. with the influence of the central nervous system isolated from the systemic circulation, the direct peripheral vascular effects of two specific, chemically distinct alpha 2-adrenergic receptor agonists, uk 14,304 and b-ht 920, were investigated in 19 dogs on total cardiopulmonary bypass with constant arterial perfusi ... | 1988 | 2895736 |
| the alpha 2-adrenoceptor agonists clonidine, tl99 and dpi enhance vasoconstrictor responses to sympathetic nerve stimulation and noradrenaline in the rat tail artery preparation. | 1. clonidine (1-30 nmol/l) enhanced the vasoconstrictor responses to sympathetic nerve stimulation in isolated perfused artery preparations from the rat tail. higher concentrations of clonidine produced vasoconstriction in most artery preparations and reduced the response to sympathetic nerve stimulation. 2. the enhancing effect of clonidine was not affected by prazosin but was blocked by idazoxan, indicating that it was due to activation of alpha 2-adrenoceptors. 3. the alpha 2-adrenoceptor ago ... | 1987 | 2896082 |
| human t-cell leukemia/lymphoma viruses: atl and aids. | | 1985 | 2897327 |
| relationship of free intracellular calcium to the cytolytic activity of entamoeba histolytica. | entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or n-acetyl-d-galactosamine-inhibitable surface lectin (gal/galnac adherence lectin). free intracellular ca2+ concentration [( ca2+]i) was measured in living amebae and target cells during amebic cytolysis of chinese hamster ovary (cho) cells and human polymorphonuclear neutrophils by utilizing the ca2+ probe fura-2 and computer-enhanced digit ... | 1988 | 2897335 |
| antimicrobial susceptibility of bordetella pertussis (part i). | in this review of the literature data are collected from the more recent studies on the susceptibility of bordetella pertussis to penicillins, cephalosporins, other beta-lactam antibiotics, aminoglycosides, tetracyclines, erythromycin, josamycin, co-trimoxazole and various other antibiotics. the methods of susceptibility testing of b. pertussis are discussed and suggestions for standardization are made. | 1988 | 2897337 |
| the high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains. | proteoglycans are constituents of the cell surface that may play important roles in the regulation of cell behavior. here we report that the 250-kda receptor subunit that binds the multifunctional protein, transforming growth factor-beta 1 (tgf-beta 1), contains chains of heparan sulfate and chondroitin sulfate and thus is a proteoglycan. digestion of tgf-beta 1-receptor complexes with glycosaminoglycan (gag)-specific degradative enzymes yield core proteins of 115-140 kda. cell monolayers that h ... | 1988 | 2897367 |
| a selective inhibitor of camp-specific phosphodiesterase, ro 20-1724, has no effect on the quantal release of acetylcholine from the mouse phrenic nerve. | the indirect twitch response of the mouse isolated phrenic nerve-diaphragm preparation, partially paralysed by tubocurarine, was restored only by about 10% by ro 20-1724 at 2 to 280 microm. the solvent vehicle, dimethylsulphoxide, also showed the same effect to a similar extent. intracellular recordings with glass microelectrodes revealed that ro 20-1724 (40 microm) affected neither the resting membrane potential, the amplitude and frequency of miniature endplate potentials nor the amplitude of ... | 1988 | 2897454 |
| apa i and sst i rflps at the insulin-like growth factor ii (igf2) locus on chromosome 11. | | 1988 | 2897668 |
| human herpesvirus 6 and the henle-koch postulates. | | 1988 | 2901654 |
| interaction between anti-influenza viral polymerase antibodies and rnp particles using the in vitro transcription process and an immunogold labelling technique. | immunogold labelling and in vitro transcription of influenza virus vrna have been used to analyse the interaction of anti-influenza polymerase antibodies with influenza-ribonucleoprotein (rnp) complexes. the polymerase proteins (p proteins) were localized exclusively at one end of the rnp segments. in the course of transcription the amount of p protein decreased significantly. the in vitro transcriptase activity y of influenza a virus rnp complexes in the presence of anti-polymerase antibodies t ... | 1988 | 2904734 |
| blockade of spinal pathways decreases pre- and postganglionic discharge differentially. | although discharge of many sympathetic nerves decreases substantially on transection of the spinal cord in cats, firing of splenic and mesenteric postganglionic nerves continues unabated (r. l. meckler and l. c. weaver, brain res. 338: 123-135, 1985; r. d. stein and l. c. weaver. j. physiol. lond. 396: 155-172, 1988). therefore, ongoing sympathetic outflow directed to the splanchnic circulation was proposed to be less dependent on supraspinal excitatory drive than that directed to other vascular ... | 1988 | 2904774 |
| pharmacological properties of the positive inotropic and alpha 1-adrenoceptor blocking agent saterinone. | the pharmacological properties of saterinone [+/-)-1,2-dihydro-5-[4-[2-hydroxy-3-[4-(2-methoxyphenyl)-1-piperazinyl] propoxy]phenyl]-6-methyl-2-oxo-3-pyridine-carbonitrile, bdf 8634) were investigated in isolated organs of the guinea pig and in human platelets. saterinone was found to be a potent antagonist at vascular alpha 1-adrenoceptors with a pa2-value of 8.46 +/- 0.12. besides its affinity for alpha 1-adrenoceptors saterinone exerted a positive inotropic effect in the isolated papillary mu ... | 1988 | 2906245 |
| [effects of prazosin, sgb-1534, dobutamine and isoproterenol on congestive heart failure in dogs]. | effects of prazosin, dobutamine, isoproterenol and sgb-1534, a new alpha-blockade, on congestive heart failure (chf) in dogs were investigated. the model was made by protease injection into the left ventricular free wall, saline loading, and dextran and methoxamine infusions. by this maneuver, left atrial pressure (lap), systemic vascular resistance (svr) and left ventricular end-diastolic pressure (lvedp) were markedly increased, aortic blood flow (aobf) was decreased, and systemic blood pressu ... | 1988 | 2906315 |
| malaria as anthropo-ecosystem. part i: general concept. | | 1988 | 2906647 |
| trials with portable screen rooms modified for use as animal-baited net traps for mosquito collection. | trials in larimer county, colorado during july and august 1984, with recreational screen rooms modified as large animal-baited mosquito traps are described. the two units tested are free-standing, portable and require no external support. in all-night trials, 462.5 mosquitoes/trap night were captured with horse bait compared with 367/trap night with cdc light traps. in 2-hour evening comparisons, mosquitoes collected per trapping period totalled 416 for horse-bait traps, 132 for light traps, and ... | 1985 | 2906667 |
| [the action of azepoxole on central alpha 2-adrenoreceptors]. | the effect of adrenomimetics on the central alpha 2-adrenoreceptors responsible for mydriasis was studied. it was found that alpha 2-adrenomimetic azepoxole (bht-933) exerts no effect on the central alpha 2-adrenoreceptors providing mydriasis. | 1988 | 2906881 |
| reducing the illegal sale of cigarettes to minors. | this study reports on an effort to stop the illegal sale of cigarettes to minors. in santa clara county, calif, 412 stores and 30 vending machines were visited by 18 minors aged 14 through 16 years with the intent to purchase cigarettes; they were successful at 74% of the stores and 100% of the vending machines. after an aggressive six-month campaign using communitywide media, direct merchant education, contact with the chief executive officers of chain stores and franchise operations owned by m ... | 1989 | 2908999 |
| effect of aging on populations of estrogen receptor-containing cells in the rat uterus. | estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. cell numbers and density were assessed in the various uterine layers. it was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, and epithelium, no significant cell loss occurred in any region. receptor content per cell clearly decreased with age in the endometrial stroma, while only in the myometrium was there ... | 1989 | 2909390 |
| protective role of serum antibody in immunity to chlamydial genital infection. | female guinea pigs were injected intraperitoneally with pooled immunoglobulin derived from animals immunized to the chlamydial agent of guinea pig inclusion conjunctivitis. genital infections in animals receiving pooled immunoglobulin from immune animals were markedly decreased with regard to the number of inclusions detected compared with control animals. these data indicated that serum-derived antibody was able to provide a degree of protection against a chlamydial genital tract infection. | 1989 | 2909492 |
| newer techniques of laryngeal reinnervation. | we relate the experience obtained in the use of the right superior laryngeal nerve (motor branch)--cricothyroid muscle pedicle flap in dogs in an attempt to reinnervate the right posterior cricoarytenoid muscle (pca). the right vocal cord was paralyzed by severance and removal of 2.5 cm of the right recurrent laryngeal nerve. evaluation 6 months postoperatively revealed the vocal fold remobilization on the right side to have an average of about one half the mobility of the left, normal side. aft ... | 1989 | 2910195 |
| polyclonal anti-idiotypes influence macrophage chemotaxis in coxsackievirus-induced murine myocarditis. | investigation into the mechanism(s) whereby monocytes-macrophages are mobilized to the myocardium of coxsackievirus (cvb3)-infected mice was approached by assessing the role of anti-idiotypic antibodies against specific antiviral igg molecules. balb/c mice preinoculated with polyclonal anti-idiotypic, syngeneic antibodies resulted in significantly decreased myocarditis after virus challenge. on the other hand, oil-induced peritoneal exudate cells obtained from virus-exposed animals demonstrated ... | 1989 | 2911021 |
| polyamines are sufficient to drive the transport of the precursor of ornithine carbamoyltransferase into rat liver mitochondria: possible effect on mitochondrial membranes. | the polyamines spermidine, spermine and putrescine, by themselves, at physiological concentrations, induce the transport of the precursor of ornithine carbamoyltransferase into isolated rat liver mitochondria. the presence of polyamines in the transport medium results in the approach of both mitochondrial membranes, suggesting a possible role of these molecules in the transport of the precursor of ornithine carbamoyltransferase into mitochondria, by the formation and/or stabilization of mitochon ... | 1989 | 2912450 |
| failure of vitamin e to protect the retina against damage resulting from bright cyclic light exposure. | cumulative light-mediated damage to the retina over a long time period may be involved in the development of age-related retinopathies. light is thought to produce retinal damage by initiating autoxidative reactions among the molecular components of the retina. experiments were therefore conducted (1) to confirm that long-term differences in cyclic light intensity affect the rate of age-related photoreceptor cell loss from the retina; and (2) to determine whether the antioxidant, vitamin e, is a ... | 1989 | 2912912 |
| aplysia synaptosomes. i. preparation and biochemical and morphological characterization of subcellular membrane fractions. | we prepared and characterized subcellular membrane fractions from the cns of aplysia californica that are enriched in isolated nerve terminals (synaptosomes). ganglia were homogenized in 1.1 m sucrose and fractionated on a 2-step sucrose gradient, yielding 50 micrograms protein/animal in the synaptosomal fraction (p3), which was enriched 3-fold in plasma membrane as compared with the initial homogenate. quantitative morphometry of electron micrographs revealed that p3 contained 25% intact synapt ... | 1989 | 2913212 |
| improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin. | as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ... | 1989 | 2914405 |
| the protective effect of thromboxane synthetase inhibition on renal function in systemic sepsis. | to study the role of thromboxane in systemic sepsis and renal failure, peritonitis was induced surgically in 22 sheep, leading to local and systemic sepsis. a selective thromboxane synthetase inhibitor, u63,557a (upjohn co, kalamazoo, mi) was given before surgery in five animals and 30 minutes after surgery in five animals. a typical picture of volume-loaded, normotensive, vasodilated septic shock developed in all animals. twenty four hours after induction of sepsis, the control group showed a m ... | 1989 | 2916566 |
| ph-dependent uptake and macrofilaricidal effects of chloroquine on adult filarial parasites in vitro. | uptake and macrofilaricidal effects of chloroquine (cq) and other aminoquinolines were found to be highly ph dependent in brugia pahangi, acanthocheilonema viteae, onchocerca volvulus, and dirofilaria immitis. using [3h]cq, it was found that all of the parasites took up more drug under alkaline conditions (rpmi 1640 at ph 8.4) than in neutral (ph 7.4) or acidic (ph 6.8) media. differences were seen in the amount of drug taken up among the filariae studied. b. pahangi and a. viteae took up 7 time ... | 1989 | 2917628 |
| hepatic structural correlates of liver fibrosis: a morphometric analysis. | liver fibrosis is an important feature of many liver diseases, and the assessment of fibrosis is essential for diagnosis and prognosis. liver needle biopsy, however, tends to sample preferentially the soft parenchyma, which poorly reflects the true extent of fibrosis. therefore, we have searched for capsule and parenchymal features that are correlated with the fibrosis. to test for these correlations, we used a morphometric analysis of a rat model of liver fibrosis. we demonstrated that, of the ... | 1989 | 2920817 |
| degradation of type ix collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells. | the degradation of type ix collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (mmps) purified from the culture medium of rheumatoid synovial cells. neither mmp-1 (collagenase) nor mmp-2 (so-called 'gelatinase') could digest type ix collagen, but mmp-3 (stromelysin) readily degraded it into smaller fragments. this suggests that mmp-3 may be responsible for the pathological degradation and/or normal turnover of type ix collage ... | 1989 | 2920840 |
| sensitivity of campylobacter pylori to colloidal bismuth subcitrate. | | 1989 | 2921366 |
| release of atrial natriuretic peptide by atrial distension. | a heterologous radioimmunoassay was used to measure the concentration of immunoreactive atrial natriuretic peptide (ianp) in plasma from the femoral artery of eight chloralose anaesthetized dogs. mitral obstruction which increased left atrial pressure by 11 cmh2o increased plasma ianp from 97 +/- 10.3 (mean +/- se) to 135 +/- 14.3 pg/ml. pulmonary vein distension increased heart rate but did not increase plasma ianp. bilateral cervical vagotomy and administration of atenolol (2 mg/kg) did not pr ... | 1985 | 2931169 |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| cellular and vessel wall morphology of cerebral cortical arterioles after short-term diabetes in adult rats. | microvascular pathology is one of the major problems associated with prolonged diabetes mellitus. the purpose of the present study was to determine if anatomical cellular pathology of the cerebral cortical microvessels could be detected very early after the onset of a streptozotocin-induced model of diabetes. male rats at age 14-16 weeks were hyperglycemic (greater than 250 mg/dl) for 4 weeks prior to scanning and transmission electron microscopic analysis of the external and internal cell and v ... | 1985 | 2935212 |
| cnbr cleavage of the light chain of human complement factor i and alignment of the fragments. | the light chain and heavy chain of reduced and alkylated human complement factor i were purified by high-pressure gel-permeation chromatography. cnbr cleavage of factor i light chain yielded four major fragments, which were purified by gel filtration. n-terminal sequence analysis of the cnbr-cleavage fragments allowed identification of 200 of the approx. 240 amino acid residues of the light chain. an alignment is proposed, based on sequence analysis of peptides obtained after cleavage at arginin ... | 1986 | 2937401 |
| effects of heparan sulfate removal on attachment and reattachment of fibroblasts and endothelial cells. | human skin fibroblasts and calf aorta endothelial cells were grown as tissue culture monolayers in the presence of [35s]sulfate in order to label the glycosaminoglycan portions of proteoglycans for investigation of their role in cell attachment. the [35s]glycosaminoglycans were then selectively removed from the cell monolayers by the addition of various glycosaminoglycan-degrading enzymes. as previously described, in contrast to trypsin treatment none of these enzymes removed any cells from the ... | 1986 | 2937448 |
| chromophore/protein interaction in bacterial sensory rhodopsin and bacteriorhodopsin. | retinal analogues with altered conjugated double bond systems or altered stereochemistry were incorporated into the phototaxis receptor sensory rhodopsin (sr) and the light-driven proton pump bacteriorhodopsin (br) from halobacterium halobium. wavelength shifts in absorption ("opsin shifts") due to analogue interaction with the protein microenvironment demonstrate that the same overall electrostatic and steric properties of the retinal binding-site structures exist in both proteins despite their ... | 1986 | 2937462 |
| cultured human endothelial cells synthesize a plasma membrane protein complex immunologically related to the platelet glycoprotein iib/iiia complex. | we have previously demonstrated that endothelial cells synthesize a plasma membrane protein indistinguishable from platelet glycoprotein (gp) iia. the present study provides evidence for a further analogy between the platelet and the endothelial cell membrane by showing that cultured endothelial cells also synthesize a membrane protein complex immunologically related to the platelet gp iib/gp iiia complex. this evidence is based on the following observations: (1) c17, a murine monoclonal antipla ... | 1986 | 2937471 |
| a follow up of t-cell subsets and of anti-m. leprae antibody titer as measured by the fla-abs test in melanesian leprosy patients under polychemotherapy. | melanesian leprosy patients from new caledonia were studied for the following parameters during the course of polychemotherapy: peripheral blood t-cell subsets, as identified in an immunofluorescence assay with monoclonal antibodies okt3 ("pan-t"), okt4 ("helper/inducer"), and okt8 ("cytotoxic-suppressor"), and anti-mycobacterium leprae antibodies in the serum, as measured by the fluorescent leprosy antibody absorption test. a group of melanesian healthy subjects with no known exposure to m. lep ... | 1986 | 2940306 |
| [risk factors in the epidemiology of dysentery]. | the general tendency of a decrease in the morbidity rates of dysentery induced by shigella sonnei and shigella flexneri (separately) from monday to the end of the week (saturday-sunday) has been revealed, and at the same time the "infection risk" for both kinds of salmonellosis has been found to fall on the last days of the week (friday-sunday). | 1986 | 2940778 |
| dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility. | in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ... | 1986 | 2942039 |
| isolation, characterization and utilization of the fifth component (c5) of porcine complement. | the fifth component (c5) of porcine complement was isolated in immunochemically pure, homogeneous and native form. the isolated component was characterized, it has a molecular weight of 187,000 (under non-reducing conditions) and two non-identical chains of 112,000 and 76,000 under reducing conditions, respectively. the isolated c5 can be used for the detection of the c5b receptors on murine macrophages. our findings are in good agreement with the results obtained using a rabbit and a human mode ... | 1986 | 2943607 |
| ultracytochemical evidence for a proton-pump adenosine triphosphatase in chick osteoclasts. | the distribution of mg++-atpase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline ph cytochemical methods at the electron-microscopic level. reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. levamisole, ouabain, and vanadate did not affect the enzymatic activity. para-chloromercuribenzoic acid (pcmb) prevented staining of mitochondria, ruffled border, and most cytoplasmic vesicles. tri-n-butyltin ... | 1986 | 2944590 |
| [effects of cycleanine dimethobromide on heart conduction system]. | | 1985 | 2945367 |
| interaction studies in mice of sch 29851, a potential non-sedating antihistamine, with commonly used therapeutic agents. | sch 29851 was studied for its ability to interact with selected drugs used in man. the non-sedating antihistamine terfenadine and the sedating antihistamine diphenhydramine were also studied for comparison. sch 29851 at 80 mg/kg po in mice, a dose about 50 times its ed50 for blocking histamine-induced paw edema in the same species, potentiated the anticonvulsant effects of diazepam. at the high dose of 320 mg/kg po, about 80 times its ed50 for antihistamine effects, sch 29851 potentiated the abi ... | 1986 | 2945410 |
| effect of mononuclear cell factors on calcium transport in sarcoplasmic reticulum vesicles. | the effect of supernatants from cultures of mitogen-stimulated human mononuclear cells on calcium transport by sarcoplasmic reticulum was examined. calcium transport was assayed by measuring the time course of calcium accumulation by sarcoplasmic reticulum incubated with supernatants from stimulated mononuclear cells was 20% less than that by vesicles exposed to control supernatants (p less than 0.001). in contrast, no difference in calcium-dependent atpase activity was noted between vesicles in ... | 1986 | 2946307 |
| structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model. | physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ... | 1986 | 2946570 |
| structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model. | physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ... | 1986 | 2946570 |
| docosahexaenoic acid status of preterm infants at birth and following feeding with human milk or formula. | the docosahexaenoic acid (dha) status of preterm infants (less than 32 wk gestation) was measured as the molar percent of dha in individual red blood cell phospholipids: 1) in cord venous blood immediately following delivery, 2) after infants were receiving greater than 60 kcal x kg x day of energy from oral-gastric feedings, and 3) at a mean of 7 wk later. infants on full feeding received either preterm human milk or formula. the dha concentration of all phospholipid classes declined between bi ... | 1986 | 2947455 |
| [effects of my-5116 on experimental animal models of type i-type iv allergic reactions and the formation of ige antibody]. | the effects of a new anti-allergic agent, my-5116: isoamyl 5,6-dihydro-7,8-dimethyl-4,5-dioxo-4h-pyrano [3,2-c] quinoline-2-carboxylate, on experimental animal models of type i approximately type iv allergic reactions and the formation of ige antibody were investigated. my-5116 administered intraperitoneally at doses of 30 and 100 mg/kg suppressed significantly the homologous pca in guinea pigs. my-5116 administered intraperitoneally at the dose of 100 mg/kg also suppressed significantly the het ... | 1986 | 2947841 |
| neurosecretion of human hypothalamic immunoreactive beta-endorphin: in vitro regulation by dopamine. | an in vitro perifusion system was used to investigate immunoreactive beta-endorphin (beta-end-i) release from adult human hypothalami in response to dopamine (da) and the da receptor antagonist haloperidol (hal). administration of a 1 microm pulse of da consistently elicited a mean (+/- se) 88 +/- 9% increase (p less than 0.05, n = 5) in beta-end-i release, whereas 1 microm hal had no effect. administration of 1 microm da during three perifusions in which 1 microm hal was added to the medium fai ... | 1987 | 2951610 |
| reversible harmless interruption of testicular blood supply in the ram. | an effective method of interrupting testicular blood flow temporarily and repeatedly in the ram has been developed. blockade of flow has been achieved mechanically by an inflatable occluder placed around the testicular artery at the level of the spermatic cord. the effect of the blockade on total testicular blood supply was investigated using doppler flowmetry and a percutaneous xenon-133 injection method. with both approaches, the blood flow changes after inflation or deflation of the occluders ... | 1987 | 2953703 |
| [a case of severe colitis with positive hemocultures for shigella flexneri]. | | 1987 | 2954108 |
| balloon-dilatable arterial banding prosthesis: experimental study. | two arterial banding prostheses were developed that could be dilated by an intraluminal balloon dilator. one prosthesis consisted of radiopaque umbilical tape with a pleat secured by four 8-0 silk sutures thinly coated with silicone type a medical adhesive. this design was used to band the main pulmonary artery in five dogs, the subclavian artery in five, and the aorta in one. the second prosthesis, a stainless steel fatigued helix encased in a siliconized shield, was used to band the main pulmo ... | 1987 | 2954182 |
| [genetic characteristics and protective properties of neamin-resistant mutant salmonella typhimurium and s. dublin of the nea(r) str(s) and nea(r) str(r) 500 classes]. | the genetic analysis of attenuated mutants, class nea(r) str(s), with the use of bacteriophage p 22 has shown that mutation rendering the mutants resistant to neamine is localized in gene nea a. in experiments with the intraperitoneal infection of mice, the appearance of this mutation in s. typhimurium and s. dublin virulent strains has been found to lead to the decrease of virulence in 100% of clones. on the basis of the data obtained in this investigation, region str-spc in s. typhimurium and ... | 1987 | 2955615 |
| [role of the m. canis carrier state of healthy animals in the epidemiology of zoonotic microsporum infection]. | | 1987 | 2956788 |
| possible cellular mechanism for cerebral vasospasm after experimental subarachnoid hemorrhage in the dog. | this study was undertaken to examine some of the cellular ionic mechanisms responsible for the cerebral vasospasm that occurs as a consequence of subarachnoid hemorrhage (sah). after cisternal injection of autologous blood we documented spasm of the basilar artery upon angiography from 4 to 7 d postictus in six dogs. when these basilar arteries were isolated we observed marked membrane depolarization and enhanced electrical spike activity compared with controls. the slope of the membrane potenti ... | 1987 | 2957393 |
| the use of angiodynography to quantify blood flow in the canine aorta. | previous attempts to quantify blood flow by means of ultrasound have been hampered by inaccurate diameter measurements and failure to account for the parabolic nature of the cross-sectional velocity profile. angiodynography (adg) provides a b-mode image of vessel walls, and the outer flow envelope is constantly monitored to provide phasic diameter measurements throughout the cardiac cycle. frequency shifts are color-coded to provide a real-time visual display of varying velocity patterns across ... | 1987 | 2957515 |
| experimental evaluation of a new albumin-impregnated knitted dacron prosthesis. | the performance of a new albumin-impregnated, knitted vascular graft stored damp in saline was assessed by a comparative study in the canine aorta. knitted grafts interposed into the descending thoracic aorta for 4 weeks included: albumin-coated and stored damp in saline (damp albuminated) (n = 6), albumin-coated and freeze-dried (dry albuminated) (n = 5), collagen-coated (n = 4), and uncoated (n = 4). the results of macroscopic and microscopic evaluation were subjected to one-way analysis of va ... | 1987 | 2962524 |
| identification of a unique lymphocyte subpopulation in the sheep uterus. | a panel of monoclonal antibodies was used to define the lymphocyte subpopulations in the sheep uterus at various stages of the oestrous cycle. a striking finding was that the majority of lymphocytes in the uterine and endometrial glandular epithelia belonged to a unique lymphocyte subpopulation that expressed the cd45r antigen but was negative for major histocompatibility complex (mhc) class ii molecules and expressed low or undetectable levels of the cd5 antigen. when examined under the electro ... | 1988 | 2962930 |
| the pattern of atrial natriuretic peptide release during ventricular tachycardia in man. | 1. plasma levels of atrial natriuretic peptide (anp) are high in many patients with tachycardia, but patterns of release with onset and termination of tachycardia and relationships to haemodynamic recordings are not clear. blood for anp measurements was therefore drawn from the coronary sinus, femoral artery and femoral vein, and simultaneous haemodynamic recordings were made in five patients before, during and after induction of stable ventricular tachycardia for 30 min. 2. tachycardia induced ... | 1987 | 2963719 |
| high affinity aldosterone binding sites (type i receptors) in rat heart. | 1. the use of the receptor stabilizing agent sodium molybdate, and of ru26988 to exclude [3h]-aldosterone binding from type ii glucocorticoid receptors, has enabled the characterization of high affinity type i aldosterone binding sites in rat atrial and ventricular cytosols. 2. in adult male and female rats the affinity of binding (kd 4 degrees c) is approximately 1-2 nmol/l for both atria and ventricles; specificity of binding is similar to that for type i sites in classical aldosterone target ... | 1987 | 2966024 |
| a proposed mechanism of action for alg and atg in severe acquired aplastic anaemia. | in this study we have shown that various batches of anti-lymphocyte globulin (alg), anti-thymocyte globulin (atg) and human intravenous immunoglobulin (iv ig) all contain antibodies with the capacity to block lymphocyte receptors for the fc region of igg, i.e., fc gamma receptors. these antibodies may exert an effect on fc gamma receptor bearing suppressor t cell function in vivo. since t cells have been implicated in the pathogenesis of acquired severe aplastic anaemia, it is conceivable that a ... | 1988 | 2967377 |