| microbial community in microbial fuel cell (mfc) medium and effluent enriched with purple photosynthetic bacterium (rhodopseudomonas sp.). | high power densities have been obtained from mfc reactors having a purple color characteristic of rhodopseudomonas. we investigated the microbial community structure and population in developed purple mfc medium (dpmm) and mfc effluent (dpme) using 16s rrna pyrosequencing. in dpmm, dominant bacteria were comamonas (44.6%), rhodopseudomonas (19.5%) and pseudomonas (17.2%). the bacterial community of dpme mainly consisted of bacteria related to rhodopseudomonas (72.2%). hydrogen oxidizing bacteria ... | 2014 | 24949257 |
| rapid identification of bacteria and candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus pcr coupled with electrospray ionization mass spectrometry. | pcr coupled with electrospray ionization mass spectrometry (pcr/esi-ms) was compared with culture for pathogen detection in peritoneal dialysis (pd)-related peritonitis. of 21 samples of pd effluent, pcr/esi-ms identified microorganisms in 18 (86%) samples, including mycobacterium tuberculosis in 1 culture-negative sample. of 15 double-positive samples, pcr/esi-ms and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. pcr/esi-ms can ... | 2014 | 24430451 |
| klebsiella pneumoniae oropharyngeal carriage in rural and urban vietnam and the effect of alcohol consumption. | community acquired k. pneumoniae pneumonia is still common in asia and is reportedly associated with alcohol use. oropharyngeal carriage of k. pneumoniae could potentially play a role in the pathogenesis of k. pneumoniae pneumonia. however, little is known regarding k. pneumoniae oropharyngeal carriage rates and risk factors. this population-based cross-sectional study explores the association of a variety of demographic and socioeconomic factors, as well as alcohol consumption with oropharyngea ... | 2014 | 24667800 |
| comparative genomic analysis of n2-fixing and non-n2-fixing paenibacillus spp.: organization, evolution and expression of the nitrogen fixation genes. | we provide here a comparative genome analysis of 31 strains within the genus paenibacillus including 11 new genomic sequences of n2-fixing strains. the heterogeneity of the 31 genomes (15 n2-fixing and 16 non-n2-fixing paenibacillus strains) was reflected in the large size of the shell genome, which makes up approximately 65.2% of the genes in pan genome. large numbers of transposable elements might be related to the heterogeneity. we discovered that a minimal and compact nif cluster comprising ... | 2014 | 24651173 |
| utilization of glyphosate as phosphate source: biochemistry and genetics of bacterial carbon-phosphorus lyase. | after several decades of use of glyphosate, the active ingredient in weed killers such as roundup, in fields, forests, and gardens, the biochemical pathway of transformation of glyphosate phosphorus to a useful phosphorus source for microorganisms has been disclosed. glyphosate is a member of a large group of chemicals, phosphonic acids or phosphonates, which are characterized by a carbon-phosphorus bond. this is in contrast to the general phosphorus compounds utilized and metabolized by microor ... | 2014 | 24600043 |
| molecular stress responses to nano-sized zero-valent iron (nzvi) particles in the soil bacterium pseudomonas stutzeri. | nanotoxicological studies were performed in vitro using the common soil bacterium pseudomonas stutzeri to assess the potentially toxic impact of commercial nano-sized zero-valent iron (nzvi) particles, which are currently used for environmental remediation projects. the phenotypic response of p. stutzeri to nzvi toxicity includes an initial insult to the cell wall, as evidenced by tem micrographs. transcriptional analyses using genes of particular relevance in cellular activity revealed that no ... | 2014 | 24586957 |
| a mass spectrometry-based hybrid method for structural modeling of protein complexes. | we describe a method that integrates data derived from different mass spectrometry (ms)-based techniques with a modeling strategy for structural characterization of protein assemblies. we encoded structural data derived from native ms, bottom-up proteomics, ion mobility-ms and chemical cross-linking ms into modeling restraints to compute the most likely structure of a protein assembly. we used the method to generate near-native models for three known structures and characterized an assembly inte ... | 2014 | 24509631 |
| a decade of crystallization drops: crystallization of the cbb3 cytochrome c oxidase from pseudomonas stutzeri. | the cbb3 cytochrome c oxidases are distant members of the superfamily of heme copper oxidases. these terminal oxidases couple o2 reduction with proton transport across the plasma membrane and, as a part of the respiratory chain, contribute to the generation of an electrochemical proton gradient. compared with other structurally characterized members of the heme copper oxidases, the recently determined cbb3 oxidase structure at 3.2 å resolution revealed significant differences in the electron sup ... | 2014 | 24488923 |
| coexistence of nitrifying, anammox and denitrifying bacteria in a sequencing batch reactor. | elevated nitrogen removal efficiencies from ammonium-rich wastewaters have been demonstrated by several applications, that combine nitritation and anammox processes. denitrification will occur simultaneously when organic carbon is also present. in this study, the activity of aerobic ammonia oxidizing, anammox and denitrifying bacteria in a full scale sequencing batch reactor, treating digester supernatants, was studied by means of batch-assays. aob and anammox activities were maximum at ph of 8. ... | 2014 | 24550899 |
| identification of nitrogen-fixing genes and gene clusters from metagenomic library of acid mine drainage. | biological nitrogen fixation is an essential function of acid mine drainage (amd) microbial communities. however, most acidophiles in amd environments are uncultured microorganisms and little is known about the diversity of nitrogen-fixing genes and structure of nif gene cluster in amd microbial communities. in this study, we used metagenomic sequencing to isolate nif genes in the amd microbial community from dexing copper mine, china. meanwhile, a metagenome microarray containing 7,776 large-in ... | 2014 | 24498417 |
| rna sequencing reveals differences between the global transcriptomes of salmonella enterica serovar enteritidis strains with high and low pathogenicities. | salmonella enterica serovar enteritidis is one of the important causes of bacterial food-borne gastroenteritis worldwide. field strains of s. enteritidis are relatively genetically homogeneous; however, they show extensive phenotypic diversity and differences in virulence potential. rna sequencing (rna-seq) was used to characterize differences in the global transcriptome between several genetically similar but phenotypically diverse poultry-associated field strains of s. enteritidis grown in lab ... | 2014 | 24271167 |
| chemical rescue and inhibition studies to determine the role of arg301 in phosphite dehydrogenase. | phosphite dehydrogenase (ptdh) catalyzes the nad(+)-dependent oxidation of phosphite to phosphate. this reaction requires the deprotonation of a water nucleophile for attack on phosphite. a crystal structure was recently solved that identified arg301 as a potential base given its proximity and orientation to the substrates and a water molecule within the active site. mutants of this residue showed its importance for efficient catalysis, with about a 100-fold loss in k cat and substantially incre ... | 2014 | 24498026 |
| culture-inappropriate antibiotic therapy decreases quality of life improvement after sinus surgery. | despite their widespread use, antibiotics have not been shown to improve chronic rhinosinusitis (crs) outcomes. we aimed to determine whether culture-inappropriate postoperative antibiotic therapy was associated with less quality-of-life (qol) improvement following functional endoscopic sinus surgery (fess). | 2014 | 24415647 |
| epidemiology of blood stream infections at a level-1 trauma care center of india. | bloodstream infections (bsis) are one of the major life-threatening infections in hospitals. they are responsible for prolonged hospital stays, high healthcare costs, and significant mortality. the epidemiology of bsis varies between hospitals necessitating analysis of local trends. few studies are available on trauma patients, who are predisposed due to the presence of multiple invasive devices. | 2014 | 24696556 |
| temporal variability of the bioaerosol background at a subway station: concentration level, size distribution, and diversity of airborne bacteria. | naturally occurring bioaerosol environments may present a challenge to biological detection-identification-monitoring (biodim) systems aiming at rapid and reliable warning of bioterrorism incidents. one way to improve the operational performance of biodim systems is to increase our understanding of relevant bioaerosol backgrounds. subway stations are enclosed public environments which may be regarded as potential bioterrorism targets. this study provides novel information concerning the temporal ... | 2014 | 24162566 |
| early-onset neonatal sepsis. | early-onset sepsis remains a common and serious problem for neonates, especially preterm infants. group b streptococcus (gbs) is the most common etiologic agent, while escherichia coli is the most common cause of mortality. current efforts toward maternal intrapartum antimicrobial prophylaxis have significantly reduced the rates of gbs disease but have been associated with increased rates of gram-negative infections, especially among very-low-birth-weight infants. the diagnosis of neonatal sepsi ... | 2014 | 24396135 |
| isolation and identification of plant growth promoting rhizobacteria from cucumber rhizosphere and their effect on plant growth promotion and disease suppression. | plant growth promoting rhizobacteria (pgpr) are the rhizosphere bacteria that may be utilized to augment plant growth and suppress plant diseases. the objectives of this study were to identify and characterize pgpr indigenous to cucumber rhizosphere in bangladesh, and to evaluate their ability to suppress phytophthora crown rot in cucumber. a total of 66 isolates were isolated, out of which 10 (ppb1, ppb2, ppb3, ppb4, ppb5, ppb8, ppb9, ppb10, ppb11, and ppb12) were selected based on their in vit ... | 2015 | 26869996 |
| isolation and identification of plant growth promoting rhizobacteria from cucumber rhizosphere and their effect on plant growth promotion and disease suppression. | plant growth promoting rhizobacteria (pgpr) are the rhizosphere bacteria that may be utilized to augment plant growth and suppress plant diseases. the objectives of this study were to identify and characterize pgpr indigenous to cucumber rhizosphere in bangladesh, and to evaluate their ability to suppress phytophthora crown rot in cucumber. a total of 66 isolates were isolated, out of which 10 (ppb1, ppb2, ppb3, ppb4, ppb5, ppb8, ppb9, ppb10, ppb11, and ppb12) were selected based on their in vit ... | 2015 | 26869996 |
| probing an interfacial surface in the cyanide dihydratase from bacillus pumilus, a spiral forming nitrilase. | nitrilases are of significant interest both due to their potential for industrial production of valuable products as well as degradation of hazardous nitrile-containing wastes. all known functional members of the nitrilase superfamily have an underlying dimer structure. the true nitrilases expand upon this basic dimer and form large spiral or helical homo-oligomers. the formation of this larger structure is linked to both the activity and substrate specificity of these nitrilases. the sequences ... | 2015 | 26779137 |
| probing an interfacial surface in the cyanide dihydratase from bacillus pumilus, a spiral forming nitrilase. | nitrilases are of significant interest both due to their potential for industrial production of valuable products as well as degradation of hazardous nitrile-containing wastes. all known functional members of the nitrilase superfamily have an underlying dimer structure. the true nitrilases expand upon this basic dimer and form large spiral or helical homo-oligomers. the formation of this larger structure is linked to both the activity and substrate specificity of these nitrilases. the sequences ... | 2015 | 26779137 |
| microbicides alter the expression and function of rnd-type efflux pump adeabc in biofilm-associated cells of acinetobacter baumannii clinical isolates. | acinetobacter baumannii is a gram-negative bacterium that causes nosocomial infections worldwide. this microbe's propensity to form biofilms allows it to persist and to survive on clinical abiotic surfaces for long periods. in fact, a. baumannii biofilm formation and its multidrug-resistant nature severely compromise our capacity to care for patients in hospital environments. in contrast, microbicides such as cetrimide (ct) and chlorhexidine (chx) play important roles in the prevention and treat ... | 2015 | 26459900 |
| microbicides alter the expression and function of rnd-type efflux pump adeabc in biofilm-associated cells of acinetobacter baumannii clinical isolates. | acinetobacter baumannii is a gram-negative bacterium that causes nosocomial infections worldwide. this microbe's propensity to form biofilms allows it to persist and to survive on clinical abiotic surfaces for long periods. in fact, a. baumannii biofilm formation and its multidrug-resistant nature severely compromise our capacity to care for patients in hospital environments. in contrast, microbicides such as cetrimide (ct) and chlorhexidine (chx) play important roles in the prevention and treat ... | 2015 | 26459900 |
| in situ analysis of a silver nanoparticle-precipitating shewanella biofilm by surface enhanced confocal raman microscopy. | shewanella oneidensis mr-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. when allowed to anaerobically colonize an ag/agcl solid interface, s. oneidensis has precipitated silver nanoparticles (agnp), thus providing the means for a surface enhanced confocal raman microscopy (secram) investi ... | 2015 | 26709923 |
| transcriptomic profiling of methylococcus capsulatus (bath) during growth with two different methane monooxygenases. | methylococcus capsulatus (bath) is a methanotroph that possesses both a membrane-embedded (pmmo) and a soluble methane monooxygenase (smmo). the expression of these two mmo's is tightly controlled by the availability of copper in the growth medium, but the underlying mechanisms and the number of genes involved in this switch in methane oxidation is not yet fully elucidated. microarray analyses were used to assess the transcriptome in cells producing either pmmo or smmo. a total of 137 genes were ... | 2015 | 26687591 |
| transcriptomic profiling of methylococcus capsulatus (bath) during growth with two different methane monooxygenases. | methylococcus capsulatus (bath) is a methanotroph that possesses both a membrane-embedded (pmmo) and a soluble methane monooxygenase (smmo). the expression of these two mmo's is tightly controlled by the availability of copper in the growth medium, but the underlying mechanisms and the number of genes involved in this switch in methane oxidation is not yet fully elucidated. microarray analyses were used to assess the transcriptome in cells producing either pmmo or smmo. a total of 137 genes were ... | 2015 | 26687591 |
| unveiling bacterial interactions through multidimensional scaling and dynamics modeling. | we propose a new strategy to identify and visualize bacterial consortia by conducting replicated culturing of environmental samples coupled with high-throughput sequencing and multidimensional scaling analysis, followed by identification of bacteria-bacteria correlations and interactions. we conducted a proof of concept assay with pine-tree resin-based media in ten replicates, which allowed detecting and visualizing dynamical bacterial associations in the form of statistically significant and ye ... | 2015 | 26671778 |
| impact of oil spills on coral reefs can be reduced by bioremediation using probiotic microbiota. | several anthropogenic factors, including contamination by oil spills, constitute a threat to coral reef health. current methodologies to remediate polluted marine environments are based on the use of chemical dispersants; however, these can be toxic to the coral holobiont. in this study, a probiotic bacterial consortium was produced from the coral mussismilia harttii and was trained to degrade water-soluble oil fractions (wsfs). additionally, we assessed the effect of wsfs on the health of m. ha ... | 2015 | 26658023 |
| antibiotic susceptibility profile of bacilli isolated from the skin of healthy humans. | in the present work, twelve bacilli were isolated from four different regions of human skin from bela population of nagpur district, india. the isolated bacilli were identified by their morphological, cultural and biochemical characteristics. seven isolates were gram negative rods, out of which five were belong to genus pseudomonas. three among the five gram positive isolates were identified as dermabactor and the remaining two bacillus. their antimicrobial susceptibility profile was determined ... | 2015 | 26691469 |
| bioaugmentation of lactobacillus delbrueckii ssp. bulgaricus tistr 895 to enhance bio-hydrogen production of rhodobacter sphaeroides kku-ps5. | bioaugmentation or an addition of the desired microorganisms or specialized microbial strains into the anaerobic digesters can enhance the performance of microbial community in the hydrogen production process. most of the studies focused on a bioaugmentation of native microorganisms capable of producing hydrogen with the dark-fermentative hydrogen producers while information on bioaugmentation of purple non-sulfur photosynthetic bacteria (pnsb) with lactic acid-producing bacteria (lab) is still ... | 2015 | 26613000 |
| is two-stage reimplantation effective for virulent pathogenic infection in a periprosthetic hip? a retrospective analysis. | to investigate the effectiveness of two-stage reimplantation using antibiotic-loaded bone cement (albc) and the risk factors associated with failure to control periprosthetic joint infection (pji). | 2015 | 26495248 |
| microbiological contamination of mobile phones of clinicians in intensive care units and neonatal care units in public hospitals in kuwait. | the objective of this study was to explore the prevalence of microbiological contamination of mobile phones that belong to clinicians in intensive care units (icus), pediatric intensive care units (picus), and neonatal care units (ncus) in all public secondary care hospitals in kuwait. the study also aimed to describe mobile phones disinfection practices as well as factors associated with mobile phone contamination. | 2015 | 26471415 |
| crystal structure of dim-1, an acquired subclass b1 metallo-β-lactamase from pseudomonas stutzeri. | metallo-β-lactamases (mbls) hydrolyze almost all classes of β-lactam antibiotic, including carbapenems-currently first choice drugs for opportunistic infections by gram-negative bacterial pathogens. mbl inhibitor development is complicated by the diversity within this group of enzymes, and by the appearance of new enzymes that continue to be identified both as chromosomal genes and on mobile genetic elements. one such newly discovered mbl is dim-1, a mobile enzyme originally discovered in the op ... | 2015 | 26451836 |
| nipple pain in breastfeeding mothers: incidence, causes and treatments. | persistent nipple pain is one of the most common reasons given by mothers for ceasing exclusive breastfeeding. we aimed to determine the frequency of nipple pain as a reason for consultation, the most common attributed aetiologies, and the effectiveness of the advice and treatment given. | 2015 | 26426034 |
| differences in bacterial community structure in two color morphs of the hawaiian reef coral montipora capitata. | corals harbor diverse bacterial associations that contribute to the health of the host. using 16s rrna pyrosequencing, we compared the bacterial communities of red and orange morphs of the hawaiian coral montipora capitata. although both color morphs shared dominant bacterial genera, weighted and unweighted unifrac analyses showed distinct bacterial communities. a single operational taxonomic unit (otu), classified as vibrio, represented the largest driver of differences between the color morphs ... | 2015 | 26253663 |
| smi2-mediated dimerization of indolylbutenones and synthesis of the myxobacterial natural product indiacen b. | the synthesis and reactivity of indole derivatives substituted in the benzene section was studied. starting materials 4- and 6-iodoindole were conveniently prepared via the batcho-leimgruber route and purified by sublimation. novel vicinally indolyl-substituted cyclopentanols with unexpected cis-configuration were formed by smi2-mediated reductive dimerization of a 4-(indol-6-yl)butenone, obtained by heck reaction. the two indolyl units appear to chelate sm(ii)/(iii) leading to a gauche-type arr ... | 2015 | 26664588 |
| safe usage of cosmetics in bangladesh: a quality perspective based on microbiological attributes. | the present review attempted to emphasize on the microbiological quality of the commonly used cosmetics item by the majority of the bangladeshi community. the abundance of contaminating microorganisms has been quantitatively discussed and the possible health risk has been focused upon usage of these items. only a very few research efforts have been conducted on the cosmetic items in bangladesh so far. the microbiological contamination aspects have been portrayed in this review using the informat ... | 2015 | 26361608 |
| seagrass (zostera marina) colonization promotes the accumulation of diazotrophic bacteria and alters the relative abundances of specific bacterial lineages involved in benthic carbon and sulfur cycling. | seagrass colonization changes the chemistry and biogeochemical cycles mediated by microbes in coastal sediments. in this study, we molecularly characterized the diazotrophic assemblages and entire bacterial community in surface sediments of a zostera marina-colonized coastal lagoon in northern china. higher nitrogenase gene (nifh) copy numbers were detected in the sediments from the vegetated region than in the sediments from the unvegetated region nearby. the nifh phylotypes detected were mostl ... | 2015 | 26209674 |
| engineering food crops to grow in harsh environments. | achieving sustainable agriculture and producing enough food for the increasing global population will require effective strategies to cope with harsh environments such as water and nutrient stress, high temperatures and compacted soils with high impedance that drastically reduce crop yield. recent advances in the understanding of the molecular, cellular and epigenetic mechanisms that orchestrate plant responses to abiotic stress will serve as the platform to engineer improved crop plants with be ... | 2015 | 26380074 |
| insights into the bacterial community and its temporal succession during the fermentation of wine grapes. | grapes harbor complex microbial communities. it is well known that yeasts, typically saccharomyces cerevisiae, and bacteria, commonly the lactic acid fermenting oenococcus oeni, work sequentially during primary and secondary wine fermentation. in addition to these main players, several microbes, often with undesirable effects on wine quality, have been found in grapes and during wine fermentation. however, still little is known about the dynamics of the microbial community during the fermentatio ... | 2015 | 26347718 |
| hypothetical protein avin_16040 as the s-layer protein of azotobacter vinelandii and its involvement in plant root surface attachment. | a proteomic analysis of a soil-dwelling, plant growth-promoting azotobacter vinelandii strain showed the presence of a protein encoded by the hypothetical avin_16040 gene when the bacterial cells were attached to the oryza sativa root surface. an avin_16040 deletion mutant demonstrated reduced cellular adherence to the root surface, surface hydrophobicity, and biofilm formation compared to those of the wild type. by atomic force microscopy (afm) analysis of the cell surface topography, the delet ... | 2015 | 26276116 |
| bacterial diversity and bioremediation potential of the highly contaminated marine sediments at el-max district (egypt, mediterranean sea). | coastal environments worldwide are threatened by the effects of pollution, a risk particularly high in semienclosed basins like the mediterranean sea that is poorly studied from bioremediation potential perspective especially in the southern coast. here, we investigated the physical, chemical, and microbiological features of hydrocarbon and heavy metals contaminated sediments collected at el-max bay (egypt). molecular and statistical approaches assessing the structure of the sediment-dwelling ba ... | 2015 | 26273661 |
| unprecedented silver resistance in clinically isolated enterobacteriaceae: major implications for burn and wound management. | increased utilization of inorganic silver as an adjunctive to many medical devices has raised concerns of emergent silver resistance in clinical bacteria. although the molecular basis for silver resistance has been previously characterized, to date, significant phenotypic expression of these genes in clinical settings is yet to be observed. here, we identified the first strains of clinical bacteria expressing silver resistance at a level that could significantly impact wound care and the use of ... | 2015 | 26014954 |
| transcriptomic and proteomic profiling of anabaena sp. strain 90 under inorganic phosphorus stress. | inorganic phosphorus (pi) is one of the main growth-limiting factors of diazotrophic cyanobacteria. due to human activity, the availability of pi has increased in water bodies, resulting in eutrophication and the formation of massive cyanobacterial blooms. in this study, we examined the molecular responses of the cyanobacterium anabaena sp. strain 90 to phosphorus deprivation, aiming at the identification of candidate genes to monitor the pi status in cyanobacteria. furthermore, this study incre ... | 2015 | 26025890 |
| genomics and ecophysiology of heterotrophic nitrogen-fixing bacteria isolated from estuarine surface water. | the ability to reduce atmospheric nitrogen (n2) to ammonia, known as n2 fixation, is a widely distributed trait among prokaryotes that accounts for an essential input of new n to a multitude of environments. nitrogenase reductase gene (nifh) composition suggests that putative n2-fixing heterotrophic organisms are widespread in marine bacterioplankton, but their autecology and ecological significance are unknown. here, we report genomic and ecophysiology data in relation to n2 fixation by three e ... | 2015 | 26152586 |
| tetrachloromethane-degrading bacterial enrichment cultures and isolates from a contaminated aquifer. | the prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (ccl₄) for more than three decades was followed by terminal restriction fragment length polymorphism (t-rflp) during pump-and-treat remediation at the contamination source. bacterial enrichments and isolates were obtained under selective anoxic conditions, and degraded 10 mg·l(-1) ccl₄, with less than 10% transient formation of chloroform. dichloromethane and chloromethane were not detected. s ... | 2015 | 27682092 |
| serr spectroelectrochemical study of cytochrome cd1 nitrite reductase co-immobilized with physiological redox partner cytochrome c552 on biocompatible metal electrodes. | cytochrome cd1 nitrite reductases (cd1nirs) catalyze the one-electron reduction of nitrite to nitric oxide. due to their catalytic reaction, cd1nirs are regarded as promising components for biosensing, bioremediation and biotechnological applications. motivated by earlier findings that catalytic activity of cd1nir from marinobacter hydrocarbonoclasticus (mhcd1) depends on the presence of its physiological redox partner, cytochrome c552 (cyt c552), we show here a detailed surface enhanced resonan ... | 2015 | 26091174 |
| rapid and reliable identification of gram-negative bacteria and gram-positive cocci by deposition of bacteria harvested from blood cultures onto the maldi-tof plate. | rapid identification of the causative agent(s) of bloodstream infections using the matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) methodology can lead to increased empirical antimicrobial therapy appropriateness. herein, we aimed at establishing an easier and simpler method, further referred to as the direct method, using bacteria harvested by serum separator tubes from positive blood cultures and placed onto the polished steel target plate for rapid identification by mal ... | 2015 | 26084329 |
| gene deletions resulting in increased nitrogen release by azotobacter vinelandii: application of a novel nitrogen biosensor. | azotobacter vinelandii is a widely studied model diazotrophic (nitrogen-fixing) bacterium and also an obligate aerobe, differentiating it from many other diazotrophs that require environments low in oxygen for the function of the nitrogenase. as a free-living bacterium, a. vinelandii has evolved enzymes and transporters to minimize the loss of fixed nitrogen to the surrounding environment. in this study, we pursued efforts to target specific enzymes and further developed screens to identify indi ... | 2015 | 25888177 |
| draft genome sequence of the toluene-degrading pseudomonas stutzeri strain st-9. | strain st-9 was isolated from toluene-contaminated soil (samaria, israel). the draft genome has an estimated size of 4.8 mb, exhibits an average g+c content of 60.37%, and is predicted to encode 4,183 proteins, including a gene cluster for aromatic hydrocarbon degradation. it is assigned to genomovar 3 of pseudomonas stutzeri. | 2015 | 26044424 |
| chronic infection by mucoid pseudomonas aeruginosa associated with dysregulation in t-cell immunity to outer membrane porin f. | pseudomonas aeruginosa (pa) is an environmental pathogen that commonly infects individuals with cystic fibrosis (cf) and non-cf bronchiectasis, impacting morbidity and mortality. to understand the pathobiology of interactions between the bacterium and host adaptive immunity and to inform rational vaccine design, it is important to understand the adaptive immune correlates of disease. | 2015 | 25789411 |
| utilization of d-lactate as an energy source supports the growth of gluconobacter oxydans. | d-lactate was identified as one of the few available organic acids that supported the growth of gluconobacter oxydans 621h in this study. interestingly, the strain used d-lactate as an energy source but not as a carbon source, unlike other lactate-utilizing bacteria. the enzymatic basis for the growth of g. oxydans 621h on d-lactate was therefore investigated. although two putative nad-independent d-lactate dehydrogenases, gox1253 and gox2071, were capable of oxidizing d-lactate, gox1253 was the ... | 2015 | 25862219 |
| identification of gram-negative bacteria and genetic resistance determinants from positive blood culture broths by use of the verigene gram-negative blood culture multiplex microarray-based molecular assay. | bloodstream infection is a serious condition associated with significant morbidity and mortality. the outcome of these infections can be positively affected by the early implementation of effective antibiotic therapy based on the identification of the infecting organism and genetic markers associated with antibiotic resistance. in this study, we evaluated the microarray-based verigene gram-negative blood culture (bc-gn) assay in the identification of 8 genus or species targets and 6 genetic resi ... | 2015 | 25994165 |
| novel dna packaging recognition in the unusual bacteriophage n15. | phage lambda's cosb packaging recognition site is tripartite, consisting of 3 ters binding sites, called r sequences. ters binding to the critical r3 site positions the terl endonuclease for nicking cosn to generate cohesive ends. the n15 cos (cos(n15)) is closely related to cos(λ), but whereas the cosb(n15) subsite has r3, it lacks the r2 and r1 sites and the ihf binding site of cosb(λ). a bioinformatic study of n15-like phages indicates that cosb(n15) also has an accessory, remote rr2 site, wh ... | 2015 | 25956737 |
| rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons. | transposon mutagenesis with next-generation sequencing (tnseq) is a powerful approach to annotate gene function in bacteria, but existing protocols for tnseq require laborious preparation of every sample before sequencing. thus, the existing protocols are not amenable to the throughput necessary to identify phenotypes and functions for the majority of genes in diverse bacteria. here, we present a method, random bar code transposon-site sequencing (rb-tnseq), which increases the throughput of mut ... | 2015 | 25968644 |
| nitrogen removal characteristics of a newly isolated indigenous aerobic denitrifier from oligotrophic drinking water reservoir, zoogloea sp. n299. | nitrogen is considered to be one of the most widespread pollutants leading to eutrophication of freshwater ecosystems, especially in drinking water reservoirs. in this study, an oligotrophic aerobic denitrifier was isolated from drinking water reservoir sediment. nitrogen removal performance was explored. the strain was identified by 16s rrna gene sequence analysis as zoogloea sp. n299. this species exhibits a periplasmic nitrate reductase gene (napa). its specific growth rate was 0.22 h-1. obvi ... | 2015 | 25946341 |
| high production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant escherichia coli. | recently, the compatible solute 1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine) has attracted considerable interest due to its great potential as a protecting agent. to overcome the drawbacks of high salinity in the traditional bioprocess of ectoine using halophilic bacteria, various attempts have been made to engineer ectoine biosynthesis in nonhalophilic bacteria. unfortunately, the yields of ectoine in these producers are still low and hardly meet the demands of large sca ... | 2015 | 25886618 |
| similar pah fate in anaerobic digesters inoculated with three microbial communities accumulating either volatile fatty acids or methane. | urban sludge produced on wastewater treatment plants are often contaminated by organic pollutants such as polycyclic aromatic hydrocarbons (pah). their removal under methanogenic conditions was already reported, but the factors influencing this removal remain unclear. here, we determined the influence of microbial communities on pah removal under controlled physico-chemical conditions. twelve mesophilic anaerobic digesters were inoculated with three microbial communities extracted from ecosystem ... | 2015 | 25874750 |
| insights into ongoing evolution of the hexachlorocyclohexane catabolic pathway from comparative genomics of ten sphingomonadaceae strains. | hexachlorocyclohexane (hch), a synthetic organochloride, was first used as a broad-acre insecticide in the 1940s, and many hch-degrading bacterial strains have been isolated from around the globe during the last 20 years. to date, the same degradation pathway (the lin pathway) has been implicated in all strains characterized, although the pathway has only been characterized intensively in two strains and for only a single hch isomer. to further elucidate the evolution of the lin pathway, we have ... | 2015 | 25850427 |
| genome sequence of organophosphorus pesticide-degrading bacterium pseudomonas stutzeri strain yc-yh1. | pseudomonas stutzeri strain yc-yh1, isolated from pesticide-polluted soil, efficiently degrades organophosphorus pesticides (opps) such as chlorpyrifos, parathion-methyl, triazophos, and parathion. here, we report the genome sequence (4.83 mb) of p. stutzeri yc-yh1 to facilitate further investigation of the opp-degrading mechanism. | 2015 | 25814604 |
| isolation and identification of indigenous plant growth promoting rhizobacteria from himalayan region of kashmir and their effect on improving growth and nutrient contents of maize (zea mays l.). | introduction and exploitation of plant growth promoting rhizobacteria (pgpr) in agro-ecosystems enhance plant-microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. the present study was conducted to isolate and identify pgprs associated with maize (zea mays l.) from twenty sites of himalayan region of hajira-rawalakot, azad jammu and kashmir (ajk), pakistan. a total of 100 isolates were isolated from these sites, out of which eight ... | 2015 | 25852667 |
| tatbc-independent tata/tat substrate interactions contribute to transport efficiency. | the tat system can transport folded, signal peptide-containing proteins (tat substrates) across energized membranes of prokaryotes and plant plastids. a twin-arginine motif in the signal peptide of tat substrates is recognized by tatc-containing complexes, and tata permits the membrane passage. often, as in the model tat systems of escherichia coli and plant plastids, a third component - tatb - is involved that resembles tata but has a higher affinity to tatc. it is not known why most tata disso ... | 2015 | 25774531 |
| assessment of microbiological contamination in the work environments of museums, archives and libraries. | museums, archives and libraries have large working environments. the goal of this study was to determine microbial contamination in these work places and estimate the influence of microclimatic parameters and total dust content on microbial contamination. in addition, research included evaluation of ergosterol concentration and fungal bioaerosol particle size distribution. numbers of micro-organisms in the air and on the surfaces in museums were higher (2.1 × 10(2)-7.0 × 10(3) cfu/m(3) and 1.4 × ... | 2015 | 26346115 |
| evolution of molybdenum nitrogenase during the transition from anaerobic to aerobic metabolism. | molybdenum nitrogenase (nif), which catalyzes the reduction of dinitrogen to ammonium, has modulated the availability of fixed nitrogen in the biosphere since early in earth's history. phylogenetic evidence indicates that oxygen (o2)-sensitive nif emerged in an anaerobic archaeon and later diversified into an aerobic bacterium. aerobic bacteria that fix n2 have adapted a number of strategies to protect nif from inactivation by o2, including spatial and temporal segregation of nif from o2 and res ... | 2015 | 25733617 |
| recent development in production and biotechnological application of microbial enzymes. | | 2015 | 25821792 |
| hadal biosphere: insight into the microbial ecosystem in the deepest ocean on earth. | hadal oceans at water depths below 6,000 m are the least-explored aquatic biosphere. the challenger deep, located in the western equatorial pacific, with a water depth of ∼11 km, is the deepest ocean on earth. microbial communities associated with waters from the sea surface to the trench bottom (0∼10,257 m) in the challenger deep were analyzed, and unprecedented trench microbial communities were identified in the hadal waters (6,000∼10,257 m) that were distinct from the abyssal microbial commun ... | 2015 | 25713387 |
| most hydrocarbonoclastic bacteria in the total environment are diazotrophic, which highlights their value in the bioremediation of hydrocarbon contaminants. | eighty-two out of the 100 hydrocarbonoclastic bacterial species that have been already isolated from oil-contaminated kuwaiti sites, characterized by 16s rrna nucleotide sequencing, and preserved in our private culture collection, grew successfully in a mineral medium free of any nitrogenous compounds with oil vapor as the sole carbon source. fifteen out of these 82 species were selected for further study based on the predominance of most of the isolates in their specific sites. all of these spe ... | 2015 | 25740314 |
| reduction of silver ions by cell free extracts of westiellopsis sp. | biosynthesis of silver nanoparticles using westiellopsis sp. (a15), a filamentous cyanobacterium belonging to the family fischerellaceae, has been demonstrated. aqueous silver ions (ag(+)) when exposed to the culture filtrate of westiellopsis were reduced in the solution, which were characterized by biophysical measures utilizing the uv-vis spectroscopy, scanning electron microscopy (sem), and ftir. the nanoparticles exhibited the maximum absorbance at 420 nm in uv-vis spectroscopy, while the se ... | 2015 | 25722724 |
| highly efficient in vitro biosynthesis of silver nanoparticles using lysinibacillus sphaericus mr-1 and their characterization. | silver nanoparticles (agnps) have been widely used in diverse fields due to their superior properties. currently the biosynthesis of agnps is in the limelight of modern nanotechnology because of its green properties. however, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized agnps. in this work, a rapid mass agnp biosynthesis method using the cell-free extract of a novel bacterial strain, lysinibacillus sphaericus mr-1, which has been isolated from a ch ... | 2015 | 27877754 |
| synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant deinococcus radiodurans, their characterization, and determination of bioactivity. | there has been rapid progress in exploring microorganisms for green synthesis of nanoparticles since microbes show extraordinary diversity in terms of species richness and niche localization. microorganisms are easy to culture using relatively inexpensive and simple nutrients under varied conditions of temperature, pressure, ph, etc. in this work, deinococcus radiodurans that possesses the ability to withstand extremely high radiation and desiccation stress has been employed for the synthesis of ... | 2015 | 25673991 |
| ecology and biotechnology of selenium-respiring bacteria. | in nature, selenium is actively cycled between oxic and anoxic habitats, and this cycle plays an important role in carbon and nitrogen mineralization through bacterial anaerobic respiration. selenium-respiring bacteria (serb) are found in geographically diverse, pristine or contaminated environments and play a pivotal role in the selenium cycle. unlike its structural analogues oxygen and sulfur, the chalcogen selenium and its microbial cycling have received much less attention by the scientific ... | 2015 | 25631289 |
| abuo, a tolc-like outer membrane protein of acinetobacter baumannii, is involved in antimicrobial and oxidative stress resistance. | although acinetobacter baumannii is well accepted as a nosocomial pathogen, only a few of the outer membrane proteins (omps) have been functionally characterized. in this study, we demonstrate the biological functions of abuo, a homolog of tolc from escherichia coli. inactivation of abuo led to increased sensitivity to high osmolarity and oxidative stress challenge. the δabuo mutant displayed increased susceptibility to antibiotics, such as amikacin, carbenicillin, ceftriaxone, meropenem, strept ... | 2015 | 25512405 |
| genomic insights into intrinsic and acquired drug resistance mechanisms in achromobacter xylosoxidans. | achromobacter xylosoxidans is an opportunistic pathogen known to be resistant to a wide range of antibiotics; however, the knowledge about the drug resistance mechanisms is limited. we used a high-throughput sequencing approach to sequence the genomes of the a. xylosoxidans type strain atcc 27061 and a clinical isolate, a. xylosoxidans x02736, and then we used different bioinformatics tools to analyze the drug resistance genes in these bacteria. we obtained the complete genome sequence for a. xy ... | 2015 | 25487802 |
| cephalosporin side chain idiosyncrasies: a case report of ceftriaxone-induced agranulocytosis and review of literature. | | 2015 | 26034758 |
| optimization of the medium for the production of extracellular amylase by the pseudomonas stutzeri isl b5 isolated from municipal solid waste. | the management of municipal solid waste is one of the major problems of the present world. the use of microbial enzymes for sustainable management of the solid waste is the need of the time. in the present study, we have isolated a potent amylase producing strain (isl b5) from municipal solid waste. the strain was identified as pseudomonas stutzeri (p. stutzeri) both biochemically and by 16s rdna sequencing. the optimization studies revealed that the strain isl b5 exhibited maximum activity in t ... | 2016 | 28096816 |
| biodegradation of 3-methyldiphenylether (mde) by hydrogenophaga atypical strain qy7-2 and cloning of the methy-oxidation gene mdeabcd. | 3-methyldiphenylether (mde) is an important alkyl-substituted diphenyl ether compound that is widely used as an intermediate in the synthesis of pyrethroid insecticides. an efficient mde-degrading strain qy7-2, identified as hydrogenophaga atypical, was isolated from activated sludge for the first time. strain qy7-2 can utilize mde as the sole carbon and energy source and completely mineralize mde. the degradation pathway of mde was proposed in the strain through metabolites identification. a ge ... | 2016 | 27995977 |
| proclat, a new bioinformatics tool for in silico protein reclassification: case study of drab, a protein coded from the dratgb operon in azospirillum brasilense. | azopirillum brasilense is a plant-growth promoting nitrogen-fixing bacteria that is used as bio-fertilizer in agriculture. since nitrogen fixation has a high-energy demand, the reduction of n2 to nh4(+) by nitrogenase occurs only under limiting conditions of nh4(+) and o2. moreover, the synthesis and activity of nitrogenase is highly regulated to prevent energy waste. in a. brasilense nitrogenase activity is regulated by the products of drag and drat. the product of the drab gene, located downst ... | 2016 | 28105917 |
| integrated anaerobic-aerobic biodegradation of multiple contaminants including chlorinated ethylenes, benzene, toluene, and dichloromethane. | complete bioremediation of soils containing multiple volatile organic compounds (vocs) remains a challenge. to explore the possibility of complete bioremediation through integrated anaerobic-aerobic biodegradation, laboratory feasibility tests followed by alternate anaerobic-aerobic and aerobic-anaerobic biodegradation tests were performed. chlorinated ethylenes, including tetrachloroethylene (pce), trichloroethylene (tce), cis-dichloroethylene (cis-dce), and vinyl chloride (vc), and dichloromet ... | 2016 | 28042183 |
| integrated anaerobic-aerobic biodegradation of multiple contaminants including chlorinated ethylenes, benzene, toluene, and dichloromethane. | complete bioremediation of soils containing multiple volatile organic compounds (vocs) remains a challenge. to explore the possibility of complete bioremediation through integrated anaerobic-aerobic biodegradation, laboratory feasibility tests followed by alternate anaerobic-aerobic and aerobic-anaerobic biodegradation tests were performed. chlorinated ethylenes, including tetrachloroethylene (pce), trichloroethylene (tce), cis-dichloroethylene (cis-dce), and vinyl chloride (vc), and dichloromet ... | 2016 | 28042183 |
| unusual five copies and dual forms of nrdb in "candidatus liberibacter asiaticus": biological implications and pcr detection application. | "candidatus liberibacter asiaticus" (clas), a non-culturable α-proteobacterium, is associated with citrus huanglongbing (hlb, yellow shoot disease) currently threatening citrus production worldwide. here, the whole genome sequence of clas strain a4 from guangdong of china was analyzed. five copies of nrdb, encoding β-subunit of ribonucleotide reductase (rnr), a critical enzyme involving bacterial proliferation, were found. three nrdb copies were in long form (nrdb(l), 1,059 bp) and two were in s ... | 2016 | 27958354 |
| 'pseudomonas saudiphocaensis' sp. nov., a new bacterial species isolated from currency notes collected during the hajj pilgrimage in 2012 at makkah, saudi arabia. | we report here the main characteristics of 'pseudomonas saudiphocaensis' strain 20_bn(t) (csur p1224), a new species of the pseudomonas genus that was isolated from currency notes collected during the hajj pilgrimage in 2012 at makkah, saudi arabia. | 2016 | 28070338 |
| 'pseudomonas saudiphocaensis' sp. nov., a new bacterial species isolated from currency notes collected during the hajj pilgrimage in 2012 at makkah, saudi arabia. | we report here the main characteristics of 'pseudomonas saudiphocaensis' strain 20_bn(t) (csur p1224), a new species of the pseudomonas genus that was isolated from currency notes collected during the hajj pilgrimage in 2012 at makkah, saudi arabia. | 2016 | 28070338 |
| disrupting ros-protection mechanism allows hydrogen peroxide to accumulate and oxidize sb(iii) to sb(v) in pseudomonas stutzeri ts44. | microbial antimonite [sb(iii)] oxidation converts toxic sb(iii) into less toxic antimonate [sb(v)] and plays an important role in the biogeochemical sb cycle. currently, little is known about the mechanisms underlying bacterial sb(iii) resistance and oxidation. | 2016 | 27884113 |
| comparison of clinico-microbiological profile and treatment outcome of in-house and referred post cataract surgery endophthalmitis in a tertiary care center in south india. | the purpose of the study is to compare the clinico-microbiological profile and treatment outcome of in-house vs referred cases of post cataract surgery endophthalmitis in a tertiary eye care facility in south india. | 2016 | 27882513 |
| polycyclic aromatic hydrocarbon-induced changes in bacterial community structure under anoxic nitrate reducing conditions. | although bacterial anaerobic degradation of mono-aromatic compounds has been characterized in depth, the degradation of polycyclic aromatic hydrocarbons (pahs) such as naphthalene has only started to be understood in sulfate reducing bacteria, and little is known about the anaerobic degradation of pahs in nitrate reducing bacteria. starting from a series of environments which had suffered different degrees of hydrocarbon pollution, we used most probable number (mpn) enumeration to detect and qua ... | 2016 | 27877167 |
| prosthetic vascular graft infection and prosthetic joint infection caused by pseudomonas stutzeri. | pseudomonas stutzeri is infrequently isolated from clinical specimens, and if isolated, more likely represents colonization or contamination rather than infection. despite this, there are dozens of case reports which describe clinically significant p. stutzeri infections at variable sites. a 69-year-old man had a p. stutzeri infection of a prosthetic vascular graft infection, which he received in panama city. he was successfully treated with a single antipseudomonal agent for 6 weeks and the rem ... | 2016 | 27942461 |
| metagenome-assembled genome sequence of pseudomonas stutzeri strain co183 isolated from a coalbed methane well. | a near-complete pseudomonas stutzeri draft genome was extracted from a coalbed metagenome. the draft genome described herein provides insight into the functional pathways encoded by this bacterium and its potential role in coalbed methane environments. | 2016 | 27811112 |
| steady at the wheel: conservative sex and the benefits of bacterial transformation. | many bacteria are highly sexual, but the reasons for their promiscuity remain obscure. did bacterial sex evolve to maximize diversity and facilitate adaptation in a changing world, or does it instead help to retain the bacterial functions that work right now? in other words, is bacterial sex innovative or conservative? our aim in this review is to integrate experimental, bioinformatic and theoretical studies to critically evaluate these alternatives, with a main focus on natural genetic transfor ... | 2016 | 27619692 |
| halotolerant rhizobacteria promote growth and enhance salinity tolerance in peanut. | use of plant growth promoting rhizobacteria (pgpr) is a promising strategy to improve the crop production under optimal or sub-optimal conditions. in the present study, five diazotrophic salt tolerant bacteria were isolated from the roots of a halophyte, arthrocnemum indicum. the isolates were partially characterized in vitro for plant growth promoting traits and evaluated for their potential to promote growth and enhanced salt tolerance in peanut. the 16s rrna gene sequence homology indicated t ... | 2016 | 27790198 |
| picodroplet partitioned whole genome amplification of low biomass samples preserves genomic diversity for metagenomic analysis. | whole genome amplification (wga) is a challenging, key step in metagenomic studies of samples containing minute amounts of dna, such as samples from low biomass environments. it is well known that multiple displacement amplification (mda), the most commonly used wga method for microbial samples, skews the genomic representation in the sample. we have combined mda with droplet microfluidics to perform the reaction in a homogeneous emulsion. each droplet in this emulsion can be considered an indiv ... | 2016 | 27716450 |
| reconstruction of a nearly complete pseudomonas draft genome sequence from a coalbed methane-produced water metagenome. | the draft genome sequence of pseudomonas stutzeri strain k35 was separated from a metagenome derived from a produced water microbial community of a coalbed methane well. the genome encodes a complete nitrogen fixation pathway and the upper and lower naphthalene degradation pathways. | 2016 | 27795237 |
| kinetics of nitrous oxide (n2o) formation and reduction by paracoccus pantotrophus. | nitrous oxide (n2o) is a powerful greenhouse gas emitted from wastewater treatment, as well as natural systems, as a result of biological nitrification and denitrification. while denitrifying bacteria can be a significant source of n2o, they can also reduce n2o to n2. more information on the kinetics of n2o formation and reduction by denitrifying bacteria is needed to predict and quantify their impact on n2o emissions. in this study, kinetic parameters were determined for paracoccus pantotrophus ... | 2016 | 27699705 |
| continuous monitoring of the liver graft temperature: relationship between bacterial contamination of the perfusion fluid and early outcome. | a potential mechanism of the infection would be an infected donor, contamination at the time of the infusion and/or packaging, back-table procedure, and finally during the transplantation, all are potential sources of infection. the aim of our study is to analyze the incidence and significance of infection in the preservation solution according with the graft temperature. the second aim was to analyze the impact graft temperature on the clinical infections and the ischemia reperfusion injury. | 2016 | 27867949 |
| next generation sequencing data of a defined microbial mock community. | generating sequence data of a defined community composed of organisms with complete reference genomes is indispensable for the benchmarking of new genome sequence analysis methods, including assembly and binning tools. moreover the validation of new sequencing library protocols and platforms to assess critical components such as sequencing errors and biases relies on such datasets. we here report the next generation metagenomic sequence data of a defined mock community (mock bacteria archaea com ... | 2016 | 27673566 |
| microbial structure and nitrogen compound conversions in aerobic granular sludge reactors with non-aeration phases and acetate pulse feeding. | a technological system was developed for efficient nitrogen removal from real digester supernatant in a single reactor with shortened aeration to increase the economical aspects of wastewater treatment. the supernatant (600 mg tkn/l, low cod/n ratio of 2.2) was treated in batch reactors with aerobic granules (gsbrs) to test how one, two, or three non-aeration phases and acetate pulse feeding in the cycle affect the morphological and microbial properties of biomass. introduction of one non-aerati ... | 2016 | 27662853 |
| titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers. | soils are facing new environmental stressors, such as titanium dioxide nanoparticles (tio2-nps). while these emerging pollutants are increasingly released into most ecosystems, including agricultural fields, their potential impacts on soil and its function remain to be investigated. here we report the response of the microbial community of an agricultural soil exposed over 90 days to tio2-nps (1 and 500 mg kg(-1) dry soil). to assess their impact on soil function, we focused on the nitrogen cycl ... | 2016 | 27659196 |
| silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. | recent advances in nanoscience and nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. silver nanoparticles (agnps) are one of the most vital and fascinating nanomaterials among several metallic nanoparticles that are involved in biomedical applications. agnps play an important role in nanoscience and nanotechnology, particularly in nanomedicine. although several noble metals have been used for various purposes, agnps have been ... | 2016 | 27649147 |
| the genetic analysis of an acinetobacter johnsonii clinical strain evidenced the presence of horizontal genetic transfer. | acinetobacter johnsonii rarely causes human infections. while most a. johnsonii isolates are susceptible to virtually all antibiotics, strains harboring a variety of β-lactamases have recently been described. an a. johnsonii aj2199 clinical strain recovered from a hospital in buenos aires produces per-2 and oxa-58. we decided to delve into its genome by obtaining the whole genome sequence of the aj2199 strain. genome comparison studies on aj2199 revealed 240 unique genes and a close relation to ... | 2016 | 27548264 |
| bacillus pumilus cyanide dihydratase mutants with higher catalytic activity. | cyanide degrading nitrilases are noted for their potential to detoxify industrial wastewater contaminated with cyanide. however, such application would benefit from an improvement to characteristics such as their catalytic activity and stability. following error-prone pcr for random mutagenesis, several cyanide dihydratase mutants from bacillus pumilus were isolated based on improved catalysis. four point mutations, k93r, d172n, a202t, and e327k were characterized and their effects on kinetics, ... | 2016 | 27570524 |
| cadmium resistance and uptake by bacterium, salmonella enterica 43c, isolated from industrial effluent. | cadmium resistant bacterium, isolated from industrial wastewater, was characterized as salmonella enterica 43c on the basis of biochemical and 16s rrna ribotyping. it is first ever reported s. enterica 43c bared extreme resistance against heavy metal consortia in order of pb(2+)>cd(2+)>as(3+)>zn(2+)>cr(6+)>cu(2+)>hg(2+). cd(2+) stress altered growth pattern of the bacterium in time dependent manner. it could remove nearly 57 % cd(2+) from the medium over a period of 8 days. kinetic and thermodyn ... | 2016 | 27491862 |
| pnas plus significance statements. | | 2016 | 27466402 |