| antibacterial activity of herpes simplex virus grown in tissue culture. | | 1963 | 14074583 |
| survival in immune and nonimmune rabbits of cr51-labeled erythrocytes modified by bacterial antigen. | | 1963 | 14080859 |
| [quaternary ammonium bases in the treatment of bacterial and mycotic eye infections]. | | 1963 | 14083282 |
| [hemorrhagic-necrotizing pancreatitis caused by a local shwartzman phenomenon. its differentiation from tryptic pancreatitis]. | | 1963 | 14095267 |
| experimental bladder neck obstruction. iii. bacteriology and histology. | | 1963 | 14102151 |
| differences in antibody response of rabbit to enterobacterial antigen after intravenous and subcutaneous injection with adjuvant. | | 1963 | 14120329 |
| [the effect of streptococcal allergen on the course of the shwartzman phenomenon]. | | 1963 | 14134918 |
| [the effect of immunization with typhoid monovaccine on the bactericidal properties of rabbit skin]. | | 1963 | 14134935 |
| [experimental studies on the effect of antibiotics on the healing of contaminated wounds. (ii. experience with terramycin)]. | | 1964 | 14148605 |
| the protein coating of red cells in experimental immunohaemolytic anaemia. | | 1964 | 14159188 |
| uncoupling of mitochondrial and bacterial respiration by beta-nitrostyrenes and benzal malononitriles. | | 1964 | 14161015 |
| [quantitative bacteriological findings in urine and kidneys of normal rabbits and in experimental pyelonephritis. (a methodologica study)]. | | 1963 | 14166427 |
| endotoxin-like activity of serum from patients with severe localized infections. | | 1964 | 14171815 |
| a substance obtained from a staphylococcus which rapidly enhances resistance to infection. i. observations with staphylococcal and e. coli infections. | | 1964 | 14176312 |
| effect of cysteine on local shwartzman phenomenon. | | 1964 | 14181084 |
| [relation of the development of inflammation to the status of the blood coagulation system]. | | 1964 | 14182495 |
| [the role of antibacterial substances in the body in the determination of antibiotics by the agar diffusion method. the action of antibacterial substances on various microorganisms]. | | 1964 | 14191810 |
| [studies on the allergic mechanism of cholelithiasis and cholecystitis with special reference to the production of allergic reactions in the gallbladder by antigen-antibody system of e. coli components]. | | 1964 | 14194460 |
| some aspects of the antibody response of rabbits to immunization with enterobacterial somatic antigens. | | 1964 | 14194672 |
| the serologic specificity of crude and purified antigen extracts of escherichia coli in hemagglutination reactions with rabbit and human antisera. | | 1964 | 14202246 |
| [effect of chlortetracycline and oxytetracycline on the course of experimental coli bacteria pyelonephritis]. | | 1964 | 14203222 |
| some factors influencing the size of pulmonary emboli. an experimental study. | | 1964 | 14203929 |
| [endotoxin as an adjuvant. ii. effect on the production of agglutinins against leptospiral antigens]. | | 1964 | 14203989 |
| effects of bacterial endotoxin on rabbit platelets. 3. comparison of platelet injury induced by thrombin and by endotoxin. | the platelet injury produced by bacterial endotoxin and thrombin have been compared in studies utilizing citrated rabbit platelet-rich plasma. endotoxin-induced platelet injury is characterized by a lag period, is progressive, and does not produce gross clot formation. thrombin-induced platelet injury is immediate, non-progressive, and is associated with clot formation. the quantity of thrombin required to produce clot formation in this citrated system is less than that required to produce relea ... | 1964 | 14208251 |
| characterization of the pyrogenicity of candida albicans, saccharomyces cerevisiae, and cryptococcus neoformans. | kobayashi, george s. (tulane university, new orleans, la.), and lorraine friedman. characterization of the pyrogenicity of candida albicans, saccharomyces cerevisiae, and cryptococcus neoformans. j. bacteriol. 88:660-666. 1964.-the intravenous injection into rabbits of 10(9) yeast cells of candida albicans, saccharomyces cerevisiae, or cryptococcus neoformans (both slightly and heavily encapsulated forms) induced a febrile response indistinguishable from that elicited by gram-negative bacterial ... | 1964 | 14208504 |
| the comparative enzymology of triosephosphate dehydrogenase. | | 1964 | 14209940 |
| properties of a lysozyme-dissociated endotoxic fraction from escherichia coli. | mergenhagen, stephan e. (national institute of dental research, bethesda, md.), and george r. martin. properties of a lysozyme-dissociated endotoxic fraction from escherichia coli. j. bacteriol. 88:1169-1174. 1964.-treatment of a phenol-water preparation of c(14)-labeled escherichia coli o91-h21 endotoxin of low solubility with lysozyme at ph 5.0 or 8.0 effected a dissociation of the preparation. such products of dissociation were equally distributed in the chloroform and water phases after extr ... | 1964 | 14219034 |
| [activation of the cell-free synthesis of hemoglobin by ribonucleic acid. ii. action of ribonucleic acid fractions of reticulocytes obtained by centrifugation in saccharose gradient]. | | 1964 | 14220697 |
| [on the prevention of postoperative adhesions between abdominal organs]. | | 1964 | 14225758 |
| some factors influencing urinary excretion of 3-hydroxyanthranilic acid. | | 1964 | 14230395 |
| [use of the reaction with formaldehyde for characterization of certain physico-chemical properties of nucleic acids]. | | 1964 | 14233962 |
| comparison of fluorochromes for the preparation of fluorescent-antibody reagents. | | 1964 | 14234812 |
| [the binding of sulfonamides on protein bodies. 3. relation of protein binding to the ph of the solution, on the composition of the sulfonamides and on the type of the protein body. (2)]. | | 1964 | 14238617 |
| studies on septicemia in extensive burns. | | 1964 | 14239695 |
| [apropos of the pathogenesis and prevention of alimentary nitrate methemoglobinemia in infants]. | | 1964 | 14249173 |
| host-parasite relationships among group a streptococci. 3. depression of reticuloendothelial function by streptococcal pyrogenic exotoxins. | hanna, edgar e. (university of minnesota, minneapolis), and dennis w. watson. host-parasite relationships among group a streptococci. iii. depression of reticuloendothelial function by streptococcal pyrogenic exotoxins. j. bacteriol. 89:154-158. 1965.-measurement of the rate of clearance of colloidal carbon from the circulating blood is an effective and simple technique for assessing the functional state of the reticuloendothelial system (res) of experimental animals. this paper concerns a study ... | 1965 | 14255656 |
| studies on the pathogenesis of the generalized shwartzman reaction. 3. trigger mechanism for the activation of the prothrombin molecule. | | 1964 | 14257961 |
| messenger ribonucleic acid activity in rabbit reticulocyte ribosomes. | | 1964 | 14262466 |
| on the nature of tolerance of endotoxin. | | 1965 | 14264511 |
| indirect fluorescent antibody technique in the serology of toxoplasma gondii. | the indirect fluorescent antibody technique has been used to titrate patients' antibody against toxoplasma gondii. rigorous tests of the immunological validity of the method were made. the reactions of the gamma globulin of human immune serum with toxoplasma gondii and fluorescent rabbit anti-human gamma globulin were thus shown to be specific. thereafter the technique could be used with confidence to detect the reaction between doubling dilutions of patient's serum and smears of unfixed toxopla ... | 1965 | 14276154 |
| relationship between particle size and biological activity of e. coli boivin endotoxin. | | 1965 | 14278175 |
| bacterial antagonism in vitro between v. cholerae and e. coli. | | 1963 | 14299483 |
| [endotoxin as an adjuvant. 3. studies on the production of a serum against the gc system]. | | 1964 | 14305630 |
| chlortetracycline and oxytetracycline in experimental e. coli pyelonephritis. | | 1965 | 14306456 |
| endotoxin effect on heterologous precipitins in immune sera. | | 1965 | 14310751 |
| immunologic studies of antigenically modified lymphocytes from patients with leukemia. | | 1965 | 14339223 |
| [pathogenicity of hemolytic strains of escherichia coli]. | | 1965 | 14348354 |
| the effect of ovarian hormones on the uterine defense mechanism during the early stages of induced infection. | | 1960 | 14400243 |
| studies on the pathogenicity of escherichia coli. ii. the effect of a substance obtained from pseudoeosinophils of rabbits on the organism. | | 1962 | 14478291 |
| the role of immunity in the pathogenesis of experimental hematogenous pyelonephritis. | hematogenous pyelonephritis was produced in rats utilizing multiple strains of escherichia coli, a strain of klebsiella pneumoniae, and a strain of proteus mirabilis. three patterns of hematogenous pyelonephritis occurred which represent an interrelationship between an immune response to the infecting bacteria and the development of obstructive uropathy as a consequence of infection. first, the course of pyelonephritis due to strains of escherichia coli was acute, self-limited, and associated wi ... | 1962 | 14496914 |
| chimeric dr fimbriae with a herpes simplex virus type 1 epitope as a model for a recombinant vaccine. | the potential of the major structural protein drae of escherichia coli dr fimbriae has been used to display an 11-amino-acid peptide of glycoprotein d derived from herpes simplex virus (hsv) type 1. the heterologous sequence mimicking an epitope from glycoprotein d was inserted in one copy into the drae gene in place of a predicted 11-amino-acid sequence in the n-terminal region of surface-exposed domain 2 within the conserved disulfide loop (from cys21 to cys53). the inserted epitope was displa ... | 2003 | 14500468 |
| identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids. | we identified and characterized a novel staphylococcal enterotoxin-like putative toxin, which is named ser. nucleotide sequencing analysis of the ser gene revealed that ser was most closely related to the seg gene. the ser gene product, ser, was successfully expressed as a recombinant protein in an escherichia coli expression system, and recombinant ser (rser) showed significant t-cell stimulation activity. the ser production in ser-harboring staphylococcus aureus strains was confirmed by wester ... | 2003 | 14500536 |
| ana o 2, a major cashew (anacardium occidentale l.) nut allergen of the legumin family. | we recently cloned and described a vicilin and showed it to be a major cashew allergen. additional ige-reactive cashew peptides of the legumin group and 2s albumin families have also been reported. here, we attempt to clone, express and characterize a second major cashew allergen. | 2003 | 14555856 |
| early development of cavernomatous vasculatures in portal venous thrombosis: morphometric kinetics in rabbit model. | we tried to establish an animal model of portal venous thrombosis in order to analyze the ensuing pathological changes of the liver. an emulsion with escherichia coli endotoxin and lipiodol-ultra-fluide was injected into the portal vein of the left anterior lobe of the rabbit liver. the target lobe and portal vein were time-sequentially examined. in the experimental groups, it was found that fibrin thrombi were formed in the portal vein within 48 h after the injection and thrombi persisted for o ... | 2003 | 14563428 |
| phase i study of detoxified escherichia coli j5 lipopolysaccharide (j5dlps)/group b meningococcal outer membrane protein (omp) complex vaccine in human subjects. | we previously observed that a detoxified escherichia coli o111, rc chemotype j5 lipopolysaccharide (j5dlps)/group b meningococcal outer membrane protein (omp) vaccine protected animals from experimental lethal sepsis when immune antibodies were given passively as treatment at the onset of fever or when vaccine was given actively as prophylaxis. to test the safety and immunogenicity of this vaccine, we administered doses of 5, 10 and 25 microg (based on dlps) of vaccine at days 0, 28 and 56 to 24 ... | 2003 | 14575770 |
| genetic and phylogenetic characterization of the type ii cyclobutane pyrimidine dimer photolyases encoded by leporipoxviruses. | shope fibroma virus and myxoma virus encode proteins predicted to be type ii photolyases. these are enzymes that catalyze light-dependent repair of cyclobutane pyrimidine dimers (cpds). when the shope fibroma virus s127l gene was expressed in an escherichia coli strain lacking functional cpd repair pathways, the expressed gene protected the bacteria from 70-75% of the ultraviolet (uv) light-induced cytotoxic dna damage. this proportion suggests that leporipoxvirus photolyases can only repair cpd ... | 2003 | 14592755 |
| [antibody sandwich enzyme-linked immunoadsorbent assay for determination of recombinant e. coli l-asparaginase in rat plasma and its pharmacokinetics]. | to establish antibody sandwich enzyme-linked immunoadsorbent assay for determination of recombinant e. coli l-asparaginase in rat plasma and study its pharmacokinetics. | 2003 | 14628454 |
| expression of the major outer membrane protein (momp) of chlamydophila abortus, chlamydophila pecorum, and chlamydia suis in escherichia coli using an arabinose-inducible plasmid vector. | the ompa genes encoding the 40 kda major outer membrane protein (momp) of chlamydophila (ch.) abortus, ch. pecorum, and chlamydia (c.) suis were cloned into the arabinose-inducible plasmid vector pbadmychis, and recombinant momps (rmomp) from the three chlamydial species were expressed at high levels in escherichia (e.) coli. the proteins lacking the 22 aa n-terminal signal peptide were expressed as insoluble cytoplasmic inclusion bodies which were readily purified using immobilized metal-affini ... | 2003 | 14633208 |
| biochemical and immunological characterization of bacterially expressed and refolded plasmodium falciparum 42-kilodalton c-terminal merozoite surface protein 1. | protection against plasmodium falciparum can be induced by vaccination in animal models with merozoite surface protein 1 (msp1), which makes this protein an attractive vaccine candidate for malaria. in an attempt to produce a product that is easily scaleable and inexpensive, we expressed the c-terminal 42 kda of msp1 (msp1(42)) in escherichia coli, refolded the protein to its native form from insoluble inclusion bodies, and tested its ability to elicit antibodies with in vitro and in vivo activi ... | 2003 | 14638762 |
| isolation and characterization of an rip (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity. | ribosome-inactivating proteins (rips) are n-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rrna, thus arresting protein synthesis at the translocation step. in the present study, a protein termed tobacco rip (trip) was isolated from tobacco (nicotiana tabacum) leaves and purified using ion exchange and gel filtration chromatography in combination with yeast ribosome depurination assays. trip has a molecular mass of 26 kd as evidenced by sodium dodecyl sulfate ... | 2004 | 14671015 |
| [cloning, expressing and functional analysis of sjmf4, a novel schistosoma japonicum gene]. | based on the phenomenon of the natural anti-schistosomiasis in microtus fortis, the sera from normal microtus fortis were employed to immunoscreen the cdna library of adult schistosoma japonicum (chinese strain), two positive clones were obtained, race technique was further applied to amplify one of the clones, and a cdna fragment with an orf was identified. sequencing revealed that it was a novel gene of schistosoma japonicum, and it was named sjmf4 (schistosoma japonicum microtus fortis 4). th ... | 2003 | 14673501 |
| high-level expression of recombinant rabbit cytochrome p450 2e1 in escherichia coli c41 and its purification. | cytochrome p450 2e1 (cyp2e1) is of great interest because of its important role in the oxidation of numerous drugs and carcinogens. the yields of cyp2e1 obtained by the traditional recombinant expression systems have been relatively poor. we report here the development of a system for high-level expression of rabbit cyp2e1 in escherichia coli strain c41 (de3). expression of the membrane-bound cyp2e1 by the plw01-p450 expression plasmid, which utilizes a t7 promoter, is markedly improved by emplo ... | 2004 | 14680963 |
| serpin production using rapid in vitro transcription/translation systems. | in vitro transcription/translation systems are becoming more useful to produce proteins and determine the protein function. expression of proteins in vitro provides a rapid and controllable system to express proteins. the ability to control the system by addition of other compounds has allowed for the production of proteins that had been difficult to produce by conventional expression systems. more recently, the development of continuous in vitro systems has allowed the production of larger quan ... | 2004 | 14698632 |
| genetic analysis of the cell division protein ftsi (pbp3): amino acid substitutions that impair septal localization of ftsi and recruitment of ftsn. | ftsi (also called pbp3) of escherichia coli is a transpeptidase required for synthesis of peptidoglycan in the division septum and is one of several proteins that localize to the septal ring. ftsi comprises a small cytoplasmic domain, a transmembrane helix, a noncatalytic domain of unknown function, and a catalytic (transpeptidase) domain. the last two domains reside in the periplasm. we used pcr to randomly mutagenize ftsi, ligated the products into a green fluorescent protein fusion vector, an ... | 2004 | 14702319 |
| role of commensal bacteria in development of gut-associated lymphoid tissues and preimmune antibody repertoire. | intestinal bacteria are required for development of gut-associated lymphoid tissues (galt), which mediate a variety of host immune functions, such as mucosal immunity and oral tolerance. in rabbits, the intestinal microflora are also required for developing the preimmune ab repertoire by promoting somatic diversification of ig genes in b cells that have migrated to galt. we studied the mechanism of bacteria-induced galt development. bacteria were introduced into rabbits in which the appendix had ... | 2004 | 14707086 |
| the rabbit as a new reservoir host of enterohemorrhagic escherichia coli. | we investigated the prevalence of enterohemorrhagic escherichia coli (ehec) in rabbits acquired from two commercial vendors and a local petting zoo. fecal samples from 34 dutch belted (db) and 15 new zealand white (nzw) rabbits were cultured; and isolates were biotyped, serotyped, tested by polymerase chain reaction (pcr), and genotyped by repetitive-element sequence-based pcr (rep-pcr). seven (25%) of 28 db rabbits acquired from one commercial source were positive for ehec, including o153:h- an ... | 2003 | 14720401 |
| mammalian d-aspartyl endopeptidase: a scavenger for noxious racemized proteins in aging. | the accumulation of d-isomers of aspartic acid (d-asp) in proteins during aging has been implicated in the pathogenesis of alzheimer's disease, cataracts, and arteriosclerosis. here, we identified a specific lactacystin-sensitive endopeptidase that cleaves the d-asp-containing protein and named it d-aspartyl endopeptidase (daep). daep has a multi-complex structure (mw: 600kda) and is localized in the inner mitochondrial membrane of mouse and rabbit, but daep activity was not detected in escheric ... | 2004 | 14741696 |
| selection of human cytochrome p450 1a2 mutants with enhanced catalytic activity for heterocyclic amine n-hydroxylation. | cytochrome p450 (p450) 1a2 is the major enzyme involved in the metabolism of 2-amino-3,5-dimethylimidazo[4,5-f]quinoline (meiq) and other heterocyclic arylamines and their bioactivation to mutagens. random mutant libraries of human p450 1a2, in which mutations were made throughout the entire open reading frame, were screened with escherichia coli dj3109pnm12, a strain designed to bioactivate meiq and detect mutagenicity of the products. mutant clones with enhanced activity were confirmed using q ... | 2004 | 14744142 |
| conformation and glycosylation of a megalin fragment correlate with nephritogenicity in heymann nephritis. | active heymann nephritis (ahn), a rat model of autoimmune glomerulonephritis, is induced by immunization with autologous megalin, a 600-kda cell surface glycoprotein isolated from crude renal extracts. recombinant proteins containing a 563-residue n-terminal sequence of megalin were obtained from escherichia coli and baculovirus-insect cell expression systems. rats immunized with the soluble, secreted protein encoded by a baculovirus construct elicited high titer anti-megalin autoantibodies and ... | 2004 | 14764706 |
| [cloning, expression and purification of rabbit metallothionein-i gene in escherichia coli]. | the cdna encoding the rabbit metallothionein-i was amplified by rt-pcr from the rabbit liver induced by cadmium and cloned into prokaryotic fusion expression vector pqe40. then it was transformed into escherichia coli m15. positive expression clones were detected by colony blotting. target protein solubility was determined by western blotting analysis. the optimal induction condition of the level of protein expression with iptg induction was established by sds-page electrophoresis and imagemaste ... | 2004 | 15022470 |
| effects of the angiotensin-converting enzyme inhibitor perindopril on endothelial injury and hemostasis in rabbit endotoxic shock. | to assess the effects of the angiotensin-converting enzyme (ace) inhibitor (acei) perindopril on prolonged endothelial cell dysfunction in a rabbit endotoxic model. | 2004 | 15024568 |
| construction of prokaryotic expression system of ureb gene from a clinical helicobacter pylori strain and identification of the recombinant protein immunity. | to clone ureb gene from a clinical isolate of helicobacter pylori and construct a prokaryotic expression system of the gene and identify immunity of the expressed recombinant protein. | 2004 | 15052678 |
| heterologous expression of a gene encoding a 35 kda protein of mycobacterium avium paratuberculosis in escherichia coli. | the full-length open reading frame coding for a potentially immunogenic 35 kda protein of mycobacterium avium paratuberculosis was generated using polymerase chain reaction technology. the gene was inserted in-frame into escherichia coli expression plasmid pqe32. the resulting recombinant plasmid ppmp35 was transformed into e. coli m15. analysis of the e. coli induced with isopropyl-beta-d-thiogalactopyranoside revealed that the protein accumulated into the cytoplasm as insoluble inclusion bodie ... | 2004 | 15074767 |
| loop relaxation, a mechanism that explains the reduced specificity of rabbit 20alpha-hydroxysteroid dehydrogenase, a member of the aldo-keto reductase superfamily. | the aldo-keto reductase rabbit 20alpha-hydroxysteroid dehydrogenase (rb20alpha-hsd; akr1c5) is less selective than other hsds, since it exerts its activity both on androgens (c19 steroids) and progestins (c21 steroids). in order to identify the molecular determinants responsible for this reduced selectivity, binary (nadph) and ternary (nadp(+)/testosterone) complex structures were solved to 1.32a and 2.08a resolution, respectively. inspection of the cofactor-binding cavity led to the identificat ... | 2004 | 15123423 |
| use of monoclonal antibodies that recognize p60 for identification of listeria monocytogenes. | listeria monocytogenes causes major food-borne outbreaks of disease worldwide. specific identification of this microorganism is of utmost importance to public health and industry. listeria species are known to secrete a 60-kda protein collectively termed p60, which is encoded by the iap (invasion-associated protein) gene and secreted in large quantities into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division. due to these properties, p60 is an idea ... | 2004 | 15138168 |
| gemifloxacin: a new fluoroquinolone. | gemifloxacin is a dual targeted fluoroquinolone with potent in vitro activity against gram-positive, -negative and atypical human pathogens--pathogens considered to be important causes of community-acquired respiratory tract infections. gemifloxacin demonstrates impressive minimal inhibitory concentrations (mic 90 ) values against clinical isolates of streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, chlamydia pneumoniae and legionella spp., with mic 90 values reported to ... | 2004 | 15155113 |
| autodisplay of active sorbitol dehydrogenase (sdh) yields a whole cell biocatalyst for the synthesis of rare sugars. | whole cell biocatalysts are attractive technological tools for the regio- and enantioselective synthesis of products, especially from substrates with several identical reactive groups. in the present study, a whole cell biocatalyst for the synthesis of rare sugars from polyalcohols was constructed. for this purpose, sorbitol dehydrogenase (sdh) from rhodobacter sphaeroides, a member of the short-chain dehydrogenase/reductase (sdr) family, was expressed on the surface of escherichia coli using au ... | 2004 | 15185373 |
| [the expression of truncated yggg protein and preparation of its polyclonal antibody]. | to express truncated yggg protein (typ) in escherichia coli and to prepare anti-type antibody. | 2004 | 15191718 |
| [preparation and characterization of anti-human il-15 monoclonal antibody]. | to prepare monoclonal antibody (mab) against human interleukin-15 (hil-15) and identify its characterization. | 2004 | 15191721 |
| immunization with pfemp1-dbl1alpha generates antibodies that disrupt rosettes and protect against the sequestration of plasmodium falciparum-infected erythrocytes. | a family of parasite antigens known as plasmodium falciparum erythrocyte membrane protein 1 (pfemp1) is believed to play an important role in the binding of infected erythrocytes to host receptors in the micro-vasculature. available data advocates the existence of a subset of very adhesive (rosetting, auto-agglutinating) and antigenic pfemp1s implicated as virulence factors. serum antibodies that disrupt rosettes are rarely found in children with severe malaria but are frequent in those with mil ... | 2004 | 15246600 |
| production of the subdomains of the plasmodium falciparum apical membrane antigen 1 ectodomain and analysis of the immune response. | the apical membrane antigen 1 of plasmodium falciparum is one of the leading candidate antigens being developed as a vaccine to prevent malaria. this merozoite transmembrane protein has an ectodomain that can be divided into three subdomains (d i, d ii, and d iii). we have previously expressed a major portion of this ectodomain and have shown that it can induce antibodies that prevent merozoite invasion into red blood cells in an in vitro growth and invasion assay. to analyze the antibody respon ... | 2004 | 15271904 |
| induction of protective immunity against lethal anthrax challenge with a patch. | transcutaneous immunization (tci) is a needle-free technique that delivers antigens and adjuvants to potent epidermal immune cells. to address critical unmet needs in biodefense against anthrax, we have designed a novel vaccine delivery system using a dry adhesive patch that simplifies administration and improves tolerability of a subunit anthrax vaccine. | 2004 | 15272406 |
| [expression of adenylate kinase of schistosoma japonicum and evaluation on the immunoreactivity of the recombinant protein]. | to express and purify the schistosoma japonicum adenylate kinase (ak) protein of which the cdna sequence was subcloned into the pet32a(+) soluble expression plasmid, and to evaluate its immunoreactivity. | 2004 | 15283267 |
| high-level expression and purification of a recombinant hbd-1 fused to lmm protein in escherichia coli. | in this work, we present the production of an active 43 aa recombinant human beta-defensin-1 (rhbd-1(43)) in escherichia coli ad202 cells using specific plmm1-rhbd-1 expression system. unique solubility properties of the c-terminal fragment of light meromyosin (lmm) allowed us to overcome foreseeable problems with isolation procedures and toxicity caused by rhbd-1 to the host organism. as a result, the majority of fusion protein (lmm-rhbd-1(43)) was obtained in the soluble state, isolated by a l ... | 2004 | 15294300 |
| mycoplasma hyopneumoniae p65 surface lipoprotein is a lipolytic enzyme with a preference for shorter-chain fatty acids. | mycoplasma hyopneumoniae is the most significant bacterial pathogen of the respiratory tract of swine. p65 is an immunodominant surface lipoprotein of m. hyopneumoniae that is specifically recognized during disease. analysis of the translated amino acid sequence of the gene encoding p65 revealed similarity to the gdsl family of lipolytic enzymes. to examine the lipolytic activity of p65, the gene was cloned and expressed in escherichia coli after truncation of the prokaryotic lipoprotein signal ... | 2004 | 15317784 |
| transcriptome of enterohemorrhagic escherichia coli o157 adhering to eukaryotic plasma membranes. | using a dna microarray, we determined changes in enterohemorrhagic escherichia coli o157:h7 gene expression during binding to plasma membranes. analysis of the complete transcriptomes of the bound bacteria revealed increased levels of stress-associated mrnas and decreased levels of mrna encoding proteins involved in translation and type iii secretion. | 2004 | 15322044 |
| biological activity, membrane-targeting modification, and crystallization of soluble human decay accelerating factor expressed in e. coli. | decay-accelerating factor (daf, cd55) is a glycophosphatidyl inositol-anchored glycoprotein that regulates the activity of c3 and c5 convertases. in addition to understanding the mechanism of complement inhibition by daf through structural studies, there is also an interest in the possible therapeutic potential of the molecule. in this report we describe the cloning, expression in escherichia coli, isolation and membrane-targeting modification of the four short consensus repeat domains of solubl ... | 2004 | 15322283 |
| penetration of newer quinolones in the empyema fluid. | the degree of penetration of newer quinolones into the pleural fluid has not been studied. the objective of the present study was to determine the degree to which moxifloxacin and levofloxacin penetrate into empyemic pleural fluid using a new rabbit model of empyema. an empyema was created via the intrapleural injection of turpentine (1 ml), followed 24 h later by instillation of 2 ml (1 x 10(10)) escherichia coli bacteria (atcc 35218) into the pleural space of new zealand white rabbits. after a ... | 2004 | 15358708 |
| tnf alpha measurement in rat and human whole blood as an in vitro method to assay pyrogens and its inhibition by dexamethasone and erythromycin. | to ensure the safety of potential drugs, pyrogen tests are traditionally performed in rabbits. new methods have been developed as alternatives to the test to reduce the use of experimental animals. among these methods there are the limulus amoebocyte lysate test and the determination of cytokine production by human leukocytes and whole blood. when exposed to a range of concentrations of endotoxins, human and rat whole blood release tnfalpha at amounts that are detectable by a commercially availa ... | 2004 | 15389671 |
| molecular cloning, genomic organization and functional characterization of a new short-chain potassium channel toxin-like peptide bmtxks4 from buthus martensii karsch(bmk). | scorpion venom contains many small polypeptide toxins, which can modulate na(+), k(+), cl(-), and ca(2+) ion-channel conductance in the cell membrane. a full-length cdna sequence encoding a novel type of k(+)-channel toxin (named bmtxks4) was first isolated and identified from a venom gland cdna library of buthus martensii karsch (bmk). the encoded precursor contains 78 amino acid residues including a putative signal peptide of 21 residues, propeptide of 11 residues, and a mature peptide of 43 r ... | 2004 | 15452884 |
| use of molecular simulation for mapping conformational cyp2e1 epitopes. | the identification of the epitopes recognized by autoantibodies against cytochrome p450s (cyps) associated with drug-induced hepatotoxicity is difficult because of their conformational nature. in the present investigation, we used a novel approach based on the analysis of the whole molecule antigenic capacity following single amino acid substitutions to identify the conformational epitopes on cyp2e1. a molecular model of cyp2e1 was generated based on the cyp2c5 crystal structure, and potential m ... | 2004 | 15456790 |
| detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor. | biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ... | 2004 | 15466560 |
| subcloning of the 200-kda porphyromonas gingivalis antigen gene and inhibition of hemagglutination by an antibody against the recombinant protein. | porphyromonas gingivalis is a major etiologic agent of periodontitis and exhibits hemagglutinating and adherence activities. we previously succeeded in molecular cloning the 200-kda cell-surface antigenic protein (200-k ap), designated pmd101, that is recognized in sera from periodontitis patients, and identified the 200-k ap as a hemagglutinin a (haga) derivative. haga is one of the hemagglutinins known to be a useful vaccine against periodontitis. haga has four large, contiguous, direct repeat ... | 2004 | 15508749 |
| enhancement of 7-methoxyresorufin o-demethylation activity of human cytochrome p450 1a2 by molecular breeding. | alkylresorufins are model substrates for cytochrome p450 (p450) 1a2. the ability of human p450 1a2 to catalyze 7-methoxyresorufin o-demethylation was improved by screening of random mutant libraries (expressed in escherichia coli) on the basis of 7-methoxyresorufin o-demethylation. after three rounds of mutagenesis and screening, the triple mutant e163k/v193m/k170q yielded a kcat > five times faster than wild type p450 1a2 in steady-state kinetic analysis using either isolated membrane fractions ... | 2004 | 15519301 |
| heterogeneous nuclear ribonucleoprotein a1 is a novel internal ribosome entry site trans-acting factor that modulates alternative initiation of translation of the fibroblast growth factor 2 mrna. | alternative initiation of translation of the human fibroblast growth factor 2 (fgf-2) mrna at five in-frame cug or aug translation initiation codons requires various rna cis-acting elements, including an internal ribosome entry site (ires). here we describe the purification of a trans-acting factor controlling fgf-2 mrna translation achieved by several biochemical purification approaches. we have identified the heterogeneous nuclear ribonucleoprotein a1 (hnrnp a1) as a factor that binds to the f ... | 2005 | 15525641 |
| expression of an immunologically reactive merozoite surface protein (msp-1(42)) in e. coli. | the 42-kda carboxyl-terminal processing fragment of plasmodium falciparum merozoite surface protein-1 (pfmsp-1(42)) is one of the anti-malarial vaccine candidate antigens. in the present study, recombinant msp-1(42) was expressed as a fusion protein in a novel e. coli host. the average yield of the recombinant protein was 48 mg/l of bacterial culture. the antigenicity and immunogenicity of the purified protein were evaluated by comparing the results with those obtained from a well-characterized ... | 2004 | 15535968 |
| molecular detection of yersinia pestis isolates of indian origin by using pla specific monoclonal antibodies. | monoclonal antibodies (mabs) were generated against the recombinant plasminogen activator (pla) protein of yersinia pestis. these mabs detected pla in all the 18 isolates of y. pestis obtained from the sputum of pneumonic plague patients and from the liver and spleen of rodents from plague-affected areas of india during 1994-1995 as well as in seven of the eight isolates obtained from rodents in the surveillance regions of hosur and palmner in india during 1998 by simple dot-elisa. in immunoblot ... | 2005 | 15582689 |
| 99mtc-labeled interleukin-8 for scintigraphic detection of pulmonary infections. | interleukin (il)-8 is a chemotactic cytokine that binds with high affinity to receptors on neutrophils. previously we showed that (99m)tc-labeled il-8 is highly suitable for scintigraphic imaging in rabbit models of im infection and of colitis. | 2004 | 15596698 |
| attaching-effacing bacteria in animals. | enteric bacteria with a demonstrable or potential ability to form attaching-effacing lesions, so-called attaching-effacing (ae) bacteria, have been found in the intestinal tracts of a wide variety of warm-blooded animal species, including man. in some host species, for example cattle, pigs, rabbits and human beings, attaching-effacing escherichia coli (aeec) have an established role as enteropathogens. in other host species, ae bacteria are of less certain significance. with continuing advances ... | 2005 | 15629476 |
| purification of enterocytozoon bieneusi from stools and production of specific antibodies. | enterocytozoon bieneusi is clinically the most significant of the microsporidia in humans, causing chronic diarrhea wasting and cholangitis in individuals with human immunodeficiency virus infection and aids. little progress on this infection has been made because of the inability to propagate e. bieneusi in vitro and in vivo, which limits the source of parasite spores to the stools of infected human patients. given the size and shape of the e. bieneusi spores (1.1 to 1.6 by 0.7 to 1.0 microm) a ... | 2005 | 15634999 |