| the origin of brainstem-spinal pathways in the north american opossum (didelphis virginiana). studies using the horseradish peroxidase method. | | 1978 | 8980723 |
| [chemical modification of epsilon-nh2 groups of lysine residues in horseradish peroxidase. accessibility of these groups to different modifying agents]. | modification of epsilon-nh2 groups of lysine residues in horseradish peroxidase by acetic, propionic, butyric, valeric and succinic anhydrides and trinitrobenzenesulfonic acid (tnbs) is carried out. all the anhydrides modify at 0 degrees c four epsilon-nh2 lysine groups, and tnbs--three groups. tnbs modifies all 6 peroxidase epsilon-nh2 groups at 40 degrees c. possible spacing of lysine epsilon-nh2 groups in the enzyme molecule is discussed. | 1978 | 656503 |
| identification of the trochlear motoneurons by retrograde transport of horseradish peroxidase. | | 1978 | 648617 |
| the organization of central auditory pathways in a reptile, iguana iguana. | the present experiments were designed to trace the central auditory pathways in an extant reptile, the new worlkd lizard--iguana iguana, utilizing anterograde axonal degeneration stained by the fink-heimer ('67) method and the retrograde axonal transport of horseradish peroxidase (lavail and lavail, '74). beginning with the projections of the auditory portion of the viiith nerve, the ascending pathways were traced through successive relay nuclei to the telencephalon. the auditory portion of the ... | 1978 | 632382 |
| abnormal transport of horseradish peroxidase from the medial gastrocnemius muscle in dystrophic mice [proceedings]. | | 1978 | 650592 |
| [immunoelectron microscopy in dermatology]. | the principle of immunoelectronmicroscopic studies using horseradish perpoxidase is described. this method, especially the peroxidase-antiperoxidase multistep technique, reveals more details about the exact localization of immunophenomena in different dermatological diseases. the results of immunological investigations performed on the ultra-structural level in bullous diseases, lupus erythermatosus, vasculitis, and psoriasis are summarized and compared with the immunofluorescent and classical e ... | 1978 | 348650 |
| uptake of horseradish peroxydase by the testis of locusta migratoria during the last larval instar; relation with variations of ecdysteroid levels in haemolymph. | by using horseradish peroxydase (hrp) as a tracer, it is shown that the gonial region of the locust testis is an "open" compartment which is almost always freely penetrated by the tracer. during the last larval instar, however, the penetration of hrp decreases and ceases at the time when high levels of ecdysteroids are detected in the haemolymph by radioimmunoassay. a cause and effect relationship between tracer uptake and hormonal level could not be demonstrated by the experiments carried out u ... | 1978 | 639097 |
| kinetic analysis of the acid-alkaline conversion of horseradish peroxidases. | the nature of the acid-alkaline conversion of horseradish peroxidases was studied by measuring four rate constants in reactions, e + h+ (k1) in equilibrium (k2) eh+ and e + h2o (k3) in equilibrium (k4) eh+ + oh-, where eh+ and e denote the acid and alkaline forms of the enzymes. the values of k1, (k2 + k3), and k4 were obtained by measuring the relaxation rates of the acid leads to alkaline and alkaline leads to acid conversions by means of th ph jump method with a stopped-flow apparatus. the va ... | 1978 | 24465 |
| [demonstration of afferent connections of the forebrain in emys orbicularis turtles by the peroxidase method]. | the transport of horseradish peroxidase (hrp) out of the injection site in the dorsal ventricular ridge was studied in turtles emys orbicularis. labeled cells in the forebrain were observed in the paleostriatum among fibers of the lateral forebrain bundle. in the thalamus most of cells containing the granular hrp reaction product were located in the n. rotundus, n. reuniens and perirotundal nuclei (n. dorso-medialis anterior, n. magnocellularis thalami, n (centralis) lateralis, n. dorso-medialis ... | 2010 | 654713 |
| proteolytic and peroxidatic reactions of commercial horseradish peroxidase with myelin basic protein. | degradation of myelin basic protein during incubations with high concentrations of horseradish peroxidase has been demonstrated [johnson & cammer (1977) j. histochem. cytochem.25, 329-336]. possible mechanisms for the interaction of the basic protein with peroxidase were investigated in the present study. because the peroxidase samples previously observed to degrade basic protein were mixtures of isoenzymes, commercial preparations of the separated isoenzymes were tested, and all three degraded ... | 1978 | 77159 |
| interaction of the hemin 2 and 4 substituents with apo horseradish peroxidase. | | 1978 | 637861 |
| calcium-related properties of horseradish peroxidase. | | 1978 | 416823 |
| localization of rabbit's flocculus purkinje cells projecting to the cerebellar lateral nucleus and the nucleus prepositus hypoglossi investigated by means of the horseradish peroxidase retrograde axonal transport. | | 1978 | 19605112 |
| neural spread of herpes simplex virus types 1 and 2 in mice after corneal or subcutaneous (footpad) inoculation. | twelve herpes simplex virus (hsv) strains, 6 of each type, were inoculated subcutaneously into the left hind foot and into the cornea of the right eye of 12-day-old swiss albino mice. the neural spread of virus to trigeminal and spinal ganglia and to brain and spinal cord was studied by demonstration of infective virus, histology and electron microscopy. type 1 and type 2 infections seemed to spread equally well by intra-axonal transport. using a protein tracer (horseradish peroxidase) injected ... | 1978 | 632838 |
| ionic strength dependence of the oxidation of iodide and ferrocyanide by compound i of horseradish peroxidase. | the kinetics of the oxidation of iodide and ferrocyanide by compound i of horseradish peroxidase have been studied at 25 degrees c as a function of ionic strength and ph. the ionic strength dependencies of the second-order rate constants are tested with an extended form of the debye-hückel equation for the activity coefficients of the reacting species. for the reaction of iodide with compound i it is shown that the ph variation of the rate constant at zero ionic strength is caused mainly by titr ... | 1978 | 624287 |
| lignin synthesis: the generation of hydrogen peroxide and superoxide by horseradish peroxidase and its stimulation by manganese (ii) and phenols. | the enzyme horseradish peroxidase (ec 1.11.1.7) catalyses oxidation of nadh. nadh oxidation is prevented by addition of the enzyme superoxide dismutase (ec 1.15.1.1) to the reaction mixture before adding peroxidase but addition of dismutase after peroxidase has little inhibitory effect. catalase (ec 1.11.1.6) inhibits peroxidase-catalysed nadh oxidation when added at any time during the reaction. apparently the peroxidase uses hydrogen peroxide (h2o2) generated by non-enzymic breakdown of nadh t ... | 1978 | 24414365 |
| the bound auxins: protection of indole-3-acetic acid from peroxidase-catalyzed oxidation. | indole-3-acetic acid (iaa) was oxidized by horseradish peroxidase, but ester and amide conjugates of iaa were not degraded. addition of indoleacetyl-myo-inositol, indoleacetyl-l-aspartate, indoleacetylglycine, indoleacetyl-l-alanine, indoleacetyl-d-alanine, or indoleacetyl-β-alanine did not affect the rate of oxidation of iaa by horseradish peroxidase. peroxidase preparations from pisum sativum l. and zea mays l. behaved similarly in that they rapidly oxidized iaa, but not conjugates found in th ... | 1978 | 24414261 |
| cytochemical localization of exogenous peroxidase in adrenal medullary cells of hamster. | the endocytosis in hamster adrenal medullary cells has been studied by intravenously administered horseradish peroxidase, which was then detected with the graham-karnovsky method. the protein uptake was investigated 30, 60, and 120 min after the peroxidase injection. the exogenous protein was accumulated mostly in the lumen of the sinusoids, around the capillaries as well as at the apical pode of chromaffin reaction product was detectable also in the chromaffin cells. the protein uptake was very ... | 1978 | 754419 |
| efferent connections of the septal area in the pigeon. | the efferent connections of the septum in the pigeon were studied with the use of anterograde (3h-leucine autoradiography) and retrograde (horseradish peroxidase histochemistry) transport methods. it was observed that the dorsal septum projects to the lateral hypothalamus, portions of the periventricular hypothalamus, dorsomedial thalamus and midbrain reticular formation. fibers from the ventral half of the septum (i.e., nucleus of the diagonal band) project to medial and lateral sectors of the ... | 1978 | 737528 |
| equilibria between horseradish peroxidase and aromatic donors. | equilibria between horseradish peroxidase and aromatic hydrogen donors have been analyzed spectrophotometrically and potentiometrically. the donors alter the peroxidase spectrum slightly but reproducibly with changes of two types. donors of the two groups compete for the same binding site with no systematic difference in affinity for the enzyme. donors with one aromatic ring are fairly loosely ligated, kd3-25 mm, but enlargement, or extension of the pi-electron system, increases the affinity. a ... | 1978 | 706949 |
| the influence of taipoxin on cholinergic synapses in the electric organ of torpedo marmorata. | the influences of the snake venom taipoxin on the cholinergic synapses of the electric organ of torpedo mamorata were investigated. 1. in ultrathin sections presynaptic membrane indentations are noted under the influence of taipoxin/nacl. 2. the presynaptic membranes often show small interruptions. 3. in taipoxin/horseradish peroxidase incubated organ pieces typical omega-shaped indentations are found, but on the postsynaptic side. 4. the vesicle density decreased under the influence of the taip ... | 1978 | 681922 |
| [anomalously high pkapp shifts in protoporphyrin ix protonization during its solubilization on the apoperoxidase from horseradish and on surfactant micelles. the effect of a nonpolar environment on the pkaff shift]. | | 1978 | 679814 |
| [electrophysiologic and anatomic demonstration of inhibitory commisural vestibular neurons in the tench (tinca tinca)]. | horseradish peroxydase was injected intracellularly in some of the medullary neurons which, in fish, exhibit a passive hyperpolarizing potential after spinal cord stimulation (this indicates that such neurons both electrical and chemical inhibitions of the mauthner cells). with positive staining, commissural vestibular neurons were identified; their axons cross the midline and they establish connexions with the mauthner cell and with vestibular neurons of both sides. thus the existence of a cros ... | 1978 | 417859 |
| distribution of neurons projecting to the retina of caiman crocodilus. | horseradish peroxidase (hrp) was injected into the vitreous of the eye, the orbital cavity, or the optic tectum of caiman crocodilus. following intravitreal injections, retrograde transport of the enzyme was observed bilaterally, but predominantly contralaterally, in a large oblong field of cells at the isthmic level of the midbrain, bounded medially by the trochlear nucleus and laterally by the nucleus isthmi. control injections of hrp into the orbital cavity and eye muscles labelled motoneuron ... | 1978 | 81087 |
| analysis of acid-base properties of peroxidase and myoglobin. | two heme-linked groups of horseradish peroxidases, characterized by pka = 5.8 (isoenzyme a) and 7.3 (isoenzyme c), are believed to be distal amino acid residues and, judging by their acid-base properties in various derivatives, influence the functions of these isoenzymes. in contrast, in myoglobin ionization of the distal histidine does not influence its reactivity. accordingly, we conclude that the interaction of the distal base with a 6th ligand is weak in myoglobin but very strong in peroxida ... | 1978 | 27955 |
| nuclear magnetic resonance studies of high-spin ferric hemoproteins. | 220 mhz proton fourier transform (ft) nmr with quadrature phase detection (qpd) technique is applied to observe largely hyperfine-shifted signals of various hemoproteins and hemoenzymes in ferric high-spin state. the binding of f-, ocn-, scn-, and ch3oh to the ferric heme iron in high-spin state in various hemoproteins has been studied by the use of ft/qpd technique at 220 mhz. the binding of formate ion to metmyoglobin (metmb) has also been studied. the spectrum of the formate complex was compa ... | 1978 | 27954 |
| [effect of n-alkylimidazoles on oxidation of o-dianizidine catalyzed by horseradish peroxidase]. | effect of a number of n-alkylimidazoles (from n-methyl to n-octylimidazole) on peroxidase oxidation of o-dianizidine at ph 8.0 is studied. alkylimidazoles studied are found to activate the reaction under given conditions, the activation type being non-competitive ka and (alpha) values are similar for all the activators, which suggests a similar mechanism of their action. similar ka values suppose an insignificant role of hydrophobic interactions in the binding of n-alkylimidazoles with the enzym ... | 1978 | 26428 |
| probing axial ligands in ferric haemoproteins: an esr study of myoglobin and horseradish peroxidase in h217o. | | 1977 | 202277 |
| the visual cortex of the opossum: the retrograde transport of horseradish peroxidase to the lateral geniculate and lateral posterior nuclei. | the visual cortex of opossum was studied by injecting horseradish peroxidase into the cortex and identifying labeled neurons in the thalamus. the results show that the lateral geniculate nucleus projects to area 17 in a topographical manner: the rostral lateral geniculate is represented in caudal striate cortex, and the dorsal extremity of the lateral geniculate, which probably corresponds to the zero vertical meridian, is represented along the border of area 18. small injections in area 17 prod ... | 1977 | 73403 |
| membrane alterations during cornification of mammalian squamous epithelia: a freeze-fracture, tracer, and thin-section study. | tight junctions (zonulae occludentes) create a pericellular barrier to the diffusion of large molecules in non-keratinizing mammalian epithelia. however, in cornifying epithelia such as the epidermis, the importance of tight-junctional elements versus secreted intercellular lipid for barrier function is uncertain. in an attempt to resolve this question, we compared membrane structure in the stratum granulosum and stratum corneum of epidermis, esophagus, and vagina of newborn and adult humans and ... | 1977 | 339780 |
| circular dichroism studies on native and artificial horseradish peroxidases. | | 1977 | 914868 |
| effect of indole-3-acetic acid on the kinetics of horseradish peroxidase catalyzed scopoletin oxidation. | the kinetics of horseradish peroxidase catalyzed of scopoletin oxidation were observed to be sigmoidal. the apparent kms for scopoletin is 0.9 mm. inhibition of scopoletin oxidation by indole-3-acetic acid (iaa) appears to be non competitive. non competitive inhibition by iaa suggests a more significant role of the peroxidase protein matrix in regulating the activity of the heme moiety. | 1977 | 923704 |
| [silymarin--an inhibitor of horseradish peroxidase (author's transl)]. | silybin and silychristin non-competitively inhibit the horseradish peroxidase in vitro. silydianin on the other hand is a competitive inhibitor of the enzyme. | 1977 | 923693 |
| nuclear magnetic resonance studies of hemoproteins. acid-alkaline transition, ligand binding characteristics, and structure of the heme environments in horseradish peroxidase. | | 1977 | 20945 |
| descending input from the pneumotaxic system to the lateral respiratory nucleus of the medulla. an anatomical study with the horseradish peroxidase technique. | | 1977 | 19605040 |
| high resolution of peroxidase-indoleacetic acid oxidase isoenzymes from horseradish by isoelectric focusing. | several improved techniques for isoelectric focusing of isoenzymes in polyacrylamide gel slabs were developed. using these techniques, three commercial sources of horseradish peroxidase were each examined with three commercial sources of carrier ampholytes to determine their respective isoenzyme profiles.a much higher degree of isoenzyme resolution was obtained than reported previously. a composite of all of the various tests gave a total of 42 peroxidase isoenzymes. commercial sources of horser ... | 1977 | 16660185 |
| a sensitive histochemical method for light-and electron-microscopic demonstration of horseradish peroxidase. | | 1977 | 915248 |
| a new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. | | 1977 | 72746 |
| fine structural and ultracytochemical studies on the lymphocytes in three types of genetic mucopolysaccharidoses. | lymphocytes from 6 patients with 3 types of genetic mucopolysaccharidoses (hurler's syndrome, hunter's syndrome and morquio's syndrome) contained numberous vacuoles in their cytoplasm. the size of the vacuoles ranged from approximately 300 nm to 750 nm. the percentage of the lymphocytes with vacuoles varied from 10% to 38%. the vacuoles showed acid phosphatase activity, which indicated their lysosommal nature. staining with dialyzed iron solution usually localized acid mucosubstance in the perip ... | 1977 | 412306 |
| differential termination of large-diameter and small-diameter primary afferent fibers in the spinal dorsal gray matter as indicated by labeling with horseradish peroxidase. | | 1977 | 19605029 |
| a simple apparatus for the injection of small (nanolitre) volumes of horseradish peroxidase. | | 1977 | 19605023 |
| peroxidase and gold complexes of lectins for double labeling of surface-binding sites by electron microscopy. | double labeling experiments were performed for visualization of the binding sites of concanavalin a and anti-ahel (the lectin from helix pomatia). the anti-ahel was labeled with the colloidal gold whereas the membrane bound concanavalin a was demonstrated by an affinity technique using horseradish peroxidase. the two markers used could be clearly distinguished electron microscopically. the specificity of the cell surface double labeling was demonstrated in the control experiments. a topological ... | 1977 | 915241 |
| the nucleus corporis pontobulbaris of the north american opossum. | the nucleus of the pontobulbar body (pbu) in the north american opossum is located, for the most part, adjacent to the motor root of the trigeminal nerve. material prepared by degeneration and autoradiographic methods shows that the pbu receives projections from the facial motor-sensory cortex, red nucleus, spinal cord and cerebellum. the latter fibers probably take origin within the fastigial nucleus. each of the afferent connections ends in a restricuted part of the pbu, but there is considera ... | 1977 | 903428 |
| concanavalin a-binding by cells of the early chick embryo. | the surfaces of cells from the early embryo of the chick were examined using electron microscope techniques for the visualization of concanavalin a-binding sites. horseradish peroxidase and ferritin labelled concanavalin a were used to determine the distribution of the binding sites. all surfaces of the epiblast and hypoblast layers which were accessible to concanavalin a showed the presence of binding sites in stage 1 embryos. the ventral surface of the epiblast showed a high lectin affinity wh ... | 1977 | 561795 |
| kinetics of antibody and immunoglobulin-producing cells appearing in popliteal lymph nodes of mice stimulated with horseradish peroxidase. | | 1977 | 561126 |
| horseradish peroxidase transport from primate dental pulps. | | 1977 | 75216 |
| an electron microscopic investigation of the surface coat of the electrocyte of electrophorus electricus. | the surface coat of the electrocyte of the main electric organ of electrophorus electricus was studied using cytochemical methods (periodic acid-silver methanamine, periodic acid-chromic acid-silver methenamine, periodic acid-thiosemicarbazide-silver proteinate, concanavalin a - horseradish peroxidase, ruthenium red, alcian-blue lanthanum nitrate, colloidal iron hydroxide and cationized ferritin). the surface of the electrocyte presents perpendicularly oriented tubular invaginations of the cell ... | 1977 | 72610 |
| cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labeled alpha-bungarotoxin. | the cytochemical localization of acetylcholine (ach) receptors at the neuromuscular junction was investigated with a procedure utilizing alpha-bungarotoxin (alpha-btx) labeled directly with horseradish peroxidase (hrp). following incubation of tissues in the conjugate and reaction for peroxidase, activity was observed on the junctional folds of the motor endplate. a uniform layer of reaction product approximately 15 nm thick occurred over the apical portions of the junctional folds. membranes at ... | 1977 | 912399 |
| vestibular projections to the nucleus intercalatus of staderini mapped by retrograde transport of horseradish peroxidase. | medullary afferent projections to the nucleus intercalatus of staderini have been studied by retrograde transport of horseradish peroxidase (hrp) from highly localized injections. this nucleus receives afferent projections particularly from the medial and descending vestibular nuclei as well as from the nucleus praepositus hypoglossi of both sides. the nucleus intercalatus of staderini represents therefore an area of integration for the vestibular systems of both sides. | 1977 | 19605012 |
| electron microscopic labeling of intercellular spaces in isolated tissue specimens with horseradish peroxidase. | | 1977 | 929637 |
| magnetic circular dichroism studies on acid and alkaline forms of horseradish peroxidase. | the heme vicinities of the acid and alkaline forms of native (fd(iii)) horseradish peroxidase were investigated in terms of the magnetic circular dichroism (mcd) spectroscopy. the mcd spectrum of the acid form of native horseradish peroxidase was characteristic of a ferric high spin heme group. the resemblance in the mcd spectrum between the acid form and acetato-iron (iii)protoporphyrin ix dimethyl ester suggests that the heme iron of the acid form has the electronic structure similar to that i ... | 1977 | 19085 |
| a perfusion method of incubation to demonstrate horseradish peroxidase in bone. | a perfusion method of incubation to show horseradish peroxidase in the bone of young mice is presented. after perfusion fixation, the incubation medium is perfused from the descending aorta into the entire lower half of the animal. from the vessels there is good penetration of the medium into all tissues. this allows the preparation of any one perfused bone to ground-, semithin-, and ultrathin sections. differences in peroxidase distribution in the entire bone suggest regional differences in vas ... | 1977 | 914657 |
| the connections of the trigeminal and facial motor nuclei in the brain of the carp (cyprinus carpio l.) as revealed by anterograde and retrograde transport of horseradish peroxidase. | the connections of the rostral and caudal parts of the trigeminal and facial motor nuclei in the carp were studied with the horseradish peroxidase technique. following ionophoretic peroxidase injections in these motor nuclei, retrogradely labeled cells were observed together with anterogradely labeled motor cell processes. several cellular areas in thalamus, cerebellum and medulla oblongata were shown to project to the v and vii motor nuclei. labeled cells were found in the inferior lobe and the ... | 1977 | 903419 |
| anatomical and physiological characterization of avian sympathetic cardiac efferents. | in the preceding paper6 describing the right sympathetic cardiac nerve of the pigeon we reported a fast compound action potential component with no chronotropic effect, and single unit recordings from sympathetic postganglionic neurons demonstrated that this action potential component was not reflected in the antidromic postganglionic latencies. electron microscopy then indicated the presence of numerous myelinated fibers in the nerve, and together these findings suggested the existence of a sub ... | 1977 | 884549 |
| nuclear magnetic resonance studies of hemoproteins. interaction of horseradish peroxidase with indolepropionic acid. | | 1977 | 891962 |
| foveal sparing. new anatomical evidence for bilateral representation of the central retina. | the pattern of retinal ganglion cell projections to the dorsal lateral geniculate nuclei has been demonstrated with horseradish peroxidase neuronography. a 1 degree-wide strip centered on the vertical meridian has been found in which ipsilaterally and contralaterally projecting ganglion cells intermingle. this strip expands to a width of 3 degrees at the fovea, since mixing of horseradish peroxidase-labeled and unlabeled ganglion cells was found in a band approximately 0.5 degrees wide along bot ... | 1977 | 407893 |
| [kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase]. | a kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase within the ph range of 3.7-9.0 has been carried out. it was shown that the reaction of o-dianisidine peroxidase oxidation obeys the michaelis--menten kinetics; the kcat and km values within the ph range used were determined. the optimum of peroxidase catalytic activity during o-dianisidine oxidation was observed at ph 5.0-6.0. the kinetic pattern of the reaction is discussed. it was demonstr ... | 1977 | 20989 |
| [the reaction of holo-, apo- and coenzyme of the peroxidase to heating (author's transl)]. | the thermal reaction of the two components of the horseradish peroxidase--the apoenzyme and the prosthetic group--with that of the holoenzyme were compared. from this we conclude that the thermal inactivation of the peroxidase in aqueous solution is not caused by a change of the apoprotein alone, but rather by participation of the entire three-dimensional structure including the prosthetic group. spectrophotometric studies of the inactivation process in the holoenzyme revealed that the absorbanc ... | 1977 | 910551 |
| ultrastructural localization of a nerve growth factor-horseradish peroxidase (ngf-hrp) coupling product after retrograde axonal transport in adrenergic neurons. | | 1977 | 69476 |
| perihypoglossal and secondary vestibular projections to lobules vi and vii of the cerebellar cortex: an hrp study. | horseradish peroxidase (hrp) was injected into (1) lobule vi; (2) lobule vii; (3) lobule vi and vii of the cat's cerebellar vermis. marked cells were found in two groups of nuclei related to the vestibulo-ocular system; (a) the three perihypoglossal nuclei: praepositus hypoglossi, nucleus of roller and intercalatus of staderini and (b) two areas of the secondary vestibular complex located within the medial and the descending vestibular nuclei. it is concluded that fibers from the perihypoglossal ... | 1977 | 19604979 |
| an enzyme-linked immunoassay of testosterone. | an enzyme-linked immunoassay of testosterone is described. the conjugation of testosterone with high specific activity horseradish per-oxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. in this work attention has been paid to keeping the assay method as simple as possible. t ... | 1977 | 919013 |
| characterization of the pigeon isthmo-tectal pathway by selective uptake and retrograde movement of radioactive compounds and by golgi-like horseradish peroxidase labeling. | | 1977 | 69469 |
| effect of mixed solvents on the formation of horseradish peroxidase compound i. the importance of diffusion-controlled reactions. | in aqueous glycerol solutions, the rate constant for the formation of compound i of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. however, whem m-chloroperbenzoic acid is used to form compound i, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. the problems in obtaining accurate information about diffusion-controlled reactions are discussed as is the possible significance of diffusion control. ethanol has been used in previo ... | 1977 | 880290 |
| slow rate transport of horseradish peroxidase in toads. | | 1977 | 69468 |
| automated fluorometric analysis of galactose in blood. | in galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. we describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. galactose is oxidized by galactose oxidase to d-galacto-hexadialdose and h2o2 and measured as the highly fluorescent condensation product of homovanillic acid formed when h2o2 is acted upon by horseradish peroxidase. the procedure is 10-fold more s ... | 1977 | 870260 |
| [thermal inactivation and storage behavior of technologically important enzymes. iii. effect of reagents added to peroxidase and lipoxygenase]. | the influence of milieu factors on the thermal inactivation of peroxidase and lipoxigenase was investigated. cationogenic, anionogenic, non-ionogenic and amphoteric tensides were more or less effective in inactivating horseradish peroxidase. most effective in this respect were lecithine and monoglyceride, both capable of swelling in water. in presence of lecithine, peroxidase was inactivated already at 0 degrees c and ph 4.0. linoleic acid was more efficient in an oxygen stream than in presence ... | 1977 | 408986 |
| histochemical and ultrastructural studies in fibrodysplasia ossificans progressiva (myositis ossificans progressiva). | by light microscopy the subdermal nodule of a patient with fibrodysplasia ossificans progressiva (fop) had a fibromatoid histologic appearance. the cytoplasm of the cells stained strongly for mannose-rich glycoprotein with the concanavalin a-horseradish peroxidase (con a-hrp) method. the tumors also exhibited abundant hyaluronidase-digestible mucopolysaccharide in the interstitium with various basic staining reagents. this material appeared to consist principally of hyaluronic acid or chondroiti ... | 1977 | 141214 |
| [thermal inactivation and storage behavior of technologically important enzymes. i. horseradish and spinach peroxidase]. | the thermal inactivation and storage behaviour for horseradish and spinach peroxidases were investigated in defined systems, in spinach also within its natural environment. the inactivation curves of either enzyme show a sharp bend which is clearly visible at low, but not at higher temperatures. the d-values were taken from the inactivation curves. z-values resulting from the d-values were 25.5 degrees c for horseradish peroxidase, 13 degrees c for isolated peroxidase and 18 degrees c for peroxi ... | 1977 | 19887 |
| evaluation of the horseradish peroxidase-scopoletin method for the measurement of hydrogen peroxide formation in biological systems. | | 1977 | 883627 |
| the organization of the pulvinar in the grey squirrel (sciurus carolinensis). i. cytoarchitecture and connections. | the posterior neocortex in the grey squirrel, sciurus carolinensis, includes an extensive region which receives projections from the pulvinar. previous studies have demonstrated that this cortical region can be subdivided on the basis of differences in cytoarchitecture and electrophysiologically defined representations of the visual field. the main purpose of the present paper was to determine whether these cortical subdivisions could be related to corresponding subdivisions in the pulvinar. the ... | 1977 | 856889 |
| a new and sensitive staining method for axonally transported horseradish peroxidase (hrp) in the pigeon visual system. | | 1977 | 67876 |
| active site models of horseradish peroxidase compound i and a cytochrome p-450 analogue: electronic structure and electric field gradients. | | 1977 | 853195 |
| mechanism of thyroxine-mediated oxidation of reduced nicotinamide adenine dinucleotide in peroxidase-h2o2 system. | the oxidation of reduced nicotinamide adenine dinucleotide (nadh) by the horseradish peroxidase (hrp)-h2o2 system is greatly increased by the addition of thyroxine or related compounds. on the basis of a study of the rate of nadh oxidation in the presence of various concentrations of thyroxine, it is clear that thyroxine acts as a catalyst for nadh oxidation. spectral changes of a hrp-h2o2 complex (compound i) indicate that thyroxine acts as an electron donor to both compounds i and ii. the rate ... | 1977 | 192281 |
| analysis and computer simulation of aerobic oxidation of reduced nicotinamide adenine dinucleotide catalyzed by horseradish peroxidase. | under suitable experimental conditions the aerobic oxidation of nadh catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. a trace amount of h2o2 present in the nadh solution brought about initial burst in the formation of oxyperoxidase. about 2 mol of oxyperoxidase was formed per mol of h2o2. when a considerable amount of the ferric enzyme still remained, the initial burst was followed by an induction phase. in ... | 1977 | 192280 |
| [catalytic properties and thermostability of horseradish peroxidase covalently bound with sepharose by carbohydrate residues of the enzyme]. | a procedure for isolation of stabilized preparations of peroxidase, covalently bound with ah-sepharose 4b by the enzyme carbohydrate residues, which had been previously oxidized by iodine acid, is proposed. the catalytic properties and thermostability of soluble oxidized and immobilized peroxidase has been studied and a comparison of these properties with those of the native enzyme has been made. it has been demonstrated that the oxidation of the carbohydrate residues dose not affect eh km value ... | 1977 | 889967 |
| [development of arrangement and degradation of the system of rough endoplasmic reticulum in antibody-producing plasma cells]. | during the immune response of the cells of murine omentum to horseradish peroxidase which had been administered intraperitoneally together together with endotoxin or endotoxoid acting as an adjuvans the plasma cells showed several different patterns of the rough endoplasmic reticulum [rer] system. irregular to bizarre rer structures occurred plasma cells during the initial phase of the immune reaction only, whereas the plasma cells found at later stages had characteristic highly organized rer sy ... | 1977 | 597922 |
| [retinal projections in the lamprey, lampetra fluviatilis (horseradish perioxidase transport method in the optic nerve)]. | after the injection of horseradish peroxidase into the eye of the lamprey l. fluviatilis, the anterograde transport of the enzyme was found in the optic tract itself reaching the optic tectum and via single fibers--the geniculate nucleus. the labeled fibers were also found in the axial optic tract terminating in the geniculate nucleus and in the nucleus of the posterior commissurae. retrograde transport and labeling of the tracer were observed in cells of the dorsal and central tegmentum. | 2006 | 331759 |
| retrograde axonal transport of horseradish peroxidase from cornea to trigeminal ganglion. | horseradish peroxidase (hrp) was dripped on the scarified left cornea of adult mice. twenty-four hours later the animals were fixed by vascular perfusion and frozen sections cut from both trigeminal ganglia. after incubation for peroxidase activity labelled nerve cells were restricted to the medial ophthalmic part of the ganglion ipsilateral to hrp administration. if the scarification was omitted no neuronal labelling was observed. this labelling of the neurons is most probably the result from a ... | 1977 | 300978 |
| plant glycoprotein biosynthesis. uridine diphosphate n-acetyl-glucosaminyltransferase from horseradish root. | a particulate enzyme preparation from horseradish root tissue was shown to catalyze the transfer of 2-acetamido-2-deoxy-d-[14c1]glucose from uridine diphosphate 2-acetamido-2-deoxy-d-[14c1]glucose to an exogenous acceptor molecule derived from horseradish peroxidase. the acceptor was produced from purified peroxidase by the action of a mixture of glycoside hydrolases covalently bound to sepharose. the membrane preparation containing the transferase was purified approximately 12-fold by aqueous t ... | 1977 | 403958 |
| polymerisation of oestradiol by potassium ferricy anide and by horseradish peroxidase. | | 1977 | 862904 |
| vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice. | the transfer of protein from the cerebral ventricles to the parenchymal bloodstream in mice was studied by electron microscopy. after perfusion with the protein tracer horseradish peroxidase (hrp; m.w. approx. 40,000) through the cerebral ventricles, the tracer penetrated the ependymal lining of the ventricles and was found in the extracellular space of the neuropil close to the ependyma. hrp was also seen in the vascular basement membrane and in endothelial vesicles opening at the abluminal end ... | 1977 | 843935 |
| amino-acid sequences of heme-linked, histidine-containing peptides of five peroxidases from horseradish and turnip. | in a previous paper we have characterized five plant peroxidases, p1, p2, p3 and p7 of turnip and horseradish isoperoxidase c by peptide mapping studies, and only found two highly homologous sequences present in all. both contained histidine. the findings supported previous suggestions of two histidine sequences nearthe peroxidase heme prostetic group. in the present paper we present the amino acid sequences around the histidine residues of all four turnip peroxidases, i. e. of 25 residues aroun ... | 1977 | 849740 |
| the organization of the facial nucleus of the brush-tailed possum (trichosurus vulpecula). | the facial nucleus of the brush-tailed possum has been studied using nissl staining and the horseradish peroxidase (hrp) retrograde tracing technique. in nissl stained sections the nucleus is seen to comprise five distinct subnuclei. injections of hrp into individual facial muscle groups have shown that these subnuclei reflect the peripheral innervation pattern of efferents from this nucleus. although in most cases, injection of hrp into a single facial muscle group resulted in the labelling of ... | 1977 | 65363 |
| efferent projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis). | the projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis) have been analyzed with anterograde degeneration and autoradiographic methods for identifying axonal projections, and with the horseradish peroxidase method for identifying the distribution of neurons from which these projections originate. the cytoarchitectonic features of the paleocortical areas which receive projections from the main and the accessory olfactory bulb have also been described. the effer ... | 1977 | 65361 |
| free radical intermediate in the n-demethylation of aminopyrine by horseradish peroxidase-hydrogen peroxide. | | 1977 | 190040 |
| sensitized photooxidation of low spin horseradish peroxidase. | | 1977 | 191009 |
| electron microscopic observations of cell coat of childhood leukemic cells using concanavalin a-horseradish peroxidase method. | | 1977 | 327742 |
| horseradish peroxidase. xxv. an electron spin resonance study of the low temperature photochemical reaction of compound i. | | 1977 | 189760 |
| protein-accumulating cells and dilated cisternae of the endoplasmic reticulum in three glucosinolate-containing genera: armoracia, capparis, drypetes. | three glucosinolate-containing species, armoracia rusticana gaertner, meyer et scherbius (brassicaceae), capparis cynophallophora l. (capparaceae) and drypetes roxburghii (wall.) hurusawa (euphorbiaceae), are shown by both light and electron microscopy to contain protein-accumulating cells (pac). the pac of armoracia and copparis (former "myrosin cells") occur as idioblasts. the pac of drypetes are usual members among axial phloem parenchyma cells rather than idioblasts. in drypetes the vacuoles ... | 1977 | 24420656 |
| involvement of malate, monophenols, and the superoxide radical in hydrogen peroxide formation by isolated cell walls from horseradish (armoracia lapathifolia gilib.). | peroxidase associated with isolated horseradish cell walls catalyzes the formation of h2o2 in the presence of nadh. the reaction is stimulated by various monophenols, especially of coniferyl alcohol. nadh can be provided by a bound malate dehydrogenase. this system is capable of polymerizing coniferyl alcohol yielding an insoluble dehydrogenation polymer. nadh was found to be oxidized by two different mechanisms, one involving mn(2+), monophenol, and the superoxide radical o2 (·-) in a reaction ... | 1977 | 24420401 |
| photochemical dimerization of ferulic acid by chloroplasts from sorghum. | sorghum (sorghum bicolor) chloroplasts, lamellar fragments, and triton x-100 solubilized preparations catalyze a blue and red light-sensitized oxidation of ferulic acid to its beta-beta-linked dimer and its hydrolysis product, the acid dimer. exogenous superoxide dismutase had no effect, and catalase and 1 to 10 mm kcn inhibited this photooxidative dimerization only in detergent-treated chloroplasts. it is postulated that the final oxidant is h(2)o(2), formed by light-induced photosystem i elect ... | 1977 | 16659796 |
| selective enzymatic hydrolysis of nerve terminal phospholipids by beta-bungarotoxin: biochemical and morphological studies. | beta-bungarotoxin, a presynaptic protein neurotoxin isolated from the venom of the snake bungarus multicinctus, modifies release of neurotransmitter at the neuromuscular junction. the toxin has a potent phospholipase activity toward both natural membranes and phospholipid liposomes. studies of ionic requirements and selective chemical modification demonstrate that beta-bungarotoxin can only modify synaptic transmission under conditions in which the phospholipase is active. morphological studies ... | 1977 | 928454 |
| technical considerations on the use of horseradish peroxidase as a neuronal marker. | | 1977 | 917271 |
| retrograde transport of horseradish peroxidase from the nucleus posterior medialis hypothalami to the hippocampus and the medial septum in the pigeon. | | 1977 | 896046 |
| hydroxylation of tyrosine by plant peroxidase and mushroom tyrosinase, with and without hydrazine, to retard the oxidation of dopa. | tyrosine was oxidized to dopa by horseradish peroxidase and mushroom tyrosinase. the first step of hydroxylation of tyrosine in the synthesis of melanin was demonstrated by isolation of dopa from the reaction mixture using hydrazine as a selective retardant. | 1977 | 410043 |
| the chemical properties of out-of-equilibrium states of proteins and the role of these states in protein functioning. | the out-of-equilibrium states of several iron-containing proteins (cytochromes c of different origin, haemoglobin, myoglobin, ferredoxin and other non-haem iron proteins, cytochrome c oxidase, horseradish peroxidase) were recorded after fast changes in the active centre (electron reduction of iron, ligand dissociation). strained states result in which the active centre has already been changed and undergone vibrational relaxation but the main part of protein globule is in the 'old', now out-of-e ... | 1977 | 215393 |
| covalent structure of the glycoprotein horseradish peroxidase (ec 1.11.1.7). | | 1976 | 1001465 |
| [detection of antinuclear factor with horseradish peroxidase-conjugated antibody]. | | 1976 | 798712 |
| centrifugal fibers to the eye in a nonavian vertebrate: source revealed by horseradish peroxidase studies. | a source of efferent fibers to the eye of snakes of the genus thamnophis has been identified by the use of the retrograde transport of horseradish peroxidase. cell bodies of the contralateral nucleus of the ventral supraoptic decussation accumulate horseradish peroxidase after intraocular but not intraorbital injections. intraocular injections also result in anterograde transport of horseradish peroxidase to retinofugal axon terminals. intraorbital injections result in accumulation of horseradis ... | 1976 | 63147 |
| rhombo-mesencephalic connections in the fast conducting electrosensory system of the mormyrid fish, gnathonemus petersii. an hrp study. | using retrograde horseradish peroxidase (hrp) axonal transport direct rhombo-mesencephalic connections were revealed in gnathonemus petersii. by hrp injection in the neuropil of the extrolateral mesencephalic ganglion the neurons of the lateral line lobe nucleus were labeled. the pathway is mainly composed of crossed fibers. | 1976 | 19604893 |