| chemical structure of the adducts formed by the oxidation of benzidine in the presence of phenols. | bioactivation of carcinogens by peroxidases has received increasing attention since the discovery of the oxidation of carcinogens by prostaglandin hydroperoxidase. benzidine and 3,5,3',5'-tetramethylbenzidine are oxidized by horseradish peroxidase and prostaglandin synthase to two-electron oxidation products (di-imines). di-imines readily react with the phenolic anti-oxidant butylated hydroxyanisole to form adducts. in this paper, we have studied the oxidation of benzidine by horseradish peroxid ... | 1982 | 7172422 |
| brain stem innervation of the caudal neurosecretory system. | the innervation of the caudal neurosecretory system of poecilia sphenops (black molly) was studied by use of the retrograde horseradish peroxidase (hrp) method. the structure of the caudal neurosecretory system in this species was well suited for application of hrp procedures. acrylamide/hrp gel implants were placed in the nucleus of the caudal neurosecretory system. two neuronal groups which contained hrp filled cells were found in the brains tem. bilateral projections originate from the dorsal ... | 1982 | 7172207 |
| [basic study on pars plana vitrectomy. part 3, effects of the pars plana vitrectomy on the blood-ocular barrier using horseradish peroxidase technique and the role of prostaglandins as mediator of these effects]. | | 1982 | 7164975 |
| distribution of cell surface saccharides and fibronectin on cultured chick myotubes: relationship to acetylcholine receptor clusters. | this study examines the distribution of glycoconjugates on the surface of cultured chick myotubes with a battery of lectins labeled with ferritin or horseradish peroxidase. in addition, the distribution of a specific glycoprotein, fibronectin, is investigated by immunocytochemistry. particular attention is paid to the localization of these substances in specialized patches on the cell surface previously shown to contain a high density of acetylcholine receptors as demonstrated with horseradish p ... | 1982 | 7160317 |
| differential accessibility to two insect neurones does not account for differences in sensitivity to alpha-bungarotoxin. | the nicotinic acetylcholine receptor probe alpha-bungarotoxin (1.0 x 10(-7) m) blocks the depolarising response to ionophoretic application of acetylcholine onto the cell body membrane of the fast coxal depressor motoneurone (df) of desheathed cockroach (periplaneta americana) metathoracic ganglia, but at the same concentration is completely ineffective in blocking the depolarising action af acetylcholine on dorsal unpaired median (dum) neurones in the same ganglion. the possibility that this is ... | 1982 | 7147226 |
| extravascular circulation in the pituitary of mugil cephalus (teleostei). | extravascular circulation in the pituitary of mugil cephalus was investigated by injecting live fish with horseradish peroxidase and studying the distribution of the enzyme in the gland. the principal components of the extravascular circulatory system are the pericapillary spaces, and, arising from them, the interlobular and circumhypophyseal spaces. extensions of these spaces penetrate the glandular parenchyma of the pars distalis, where they merge with pericellular spaces. in the neurohypophys ... | 1982 | 7127409 |
| ratfish retina--intracellular recordings and hrp injections in an isolated, superfused all-rod retina. | hyperpolarizing responses to light were studied by intracellular recording in the isolated, superfused retina of the ratfish (hydrolagus colliei). two of these hyperpolarizing units were identified as horizontal cells by iontophoretic injection of horseradish peroxidase (hrp). these cells had rather large cell bodies (15 x 30 micrometer), elliptical dendritic arborizations measuring 150 x 300 micrometers and no axons. since their physiological receptive fields were found to be at least 2.15 mm i ... | 1982 | 7123872 |
| optic tract cells projecting to the retina in the teleost, pantodon buchholzi. | horseradish peroxidase was employed to trace retino-fugal and retino-petal connections in the teleost fish, pantodon buchholzi. most of the reciprocal connections found were within the range also observed in previously studied species of teleosts. of particular interest is the discovery of cells located within the optic tract and projecting to the retina. these neurons were investigated electron microscopically. | 1982 | 7116426 |
| intrinsic neurons and neural connections of the pineal organ of the house sparrow, passer domesticus, as revealed by anterograde and retrograde transport of horseradish peroxidase. | in passer domesticus, intrapineal nerve cells were labeled by uptake of microiontophoretically administered horseradish peroxidase (hrp). unipolar nerve cells with a dichotomously branching stem process are the main source of the dominant pinelaofugal component of the pineal tract, whereas multipolar and bipolar neurons appear to represent interneurons. hrp-labeled nerve fibers are observed in the distal division (end-piece) of the pineal organ; they can be regarded either as processes of intrap ... | 1982 | 7083302 |
| [intracellular horseradish peroxidase injection study of connections between primary afferents and spinal cord motor neurons in the frog]. | simultaneous intracellular penetration of a frog motoneuron and a single primary afferent fibre monosynaptically connected with it was used for potential recording followed by hrp injection. subsequent histological reconstruction of labelled motoneurons and their afferents revealed numerous contacts between them. these contacts were found both on the motoneuronal soma and dendrites, being most numerous in the proximal parts of dendrites and in the second-third-order branches. recurrent collatera ... | 1982 | 7063084 |
| elisa for antibody measurement: aspects related to data expression. | in an elisa for antitoxoplasma igg (antigen-coated polystyrene beads, horseradish peroxidase-coupled igg or staphylococcal protein a), 3 modes of expressing the analytical results were considered, i.e. end-point antibody titre, untransformed absorbance reading at a single sample dilution, and antibody-activity unit from a calibration response curve (reference sera as calibrators). criteria of merit for evaluation were defined as (a) stability of data under various conditions relating to both cha ... | 1982 | 7057041 |
| centrifugal innervation of the retina by luteinizing hormone releasing hormone (lhrh)-immunoreactive telencephalic neurons in teleostean fishes. | in cichlid, poecilid and centrarchid fishes luteinizing hormone releasing hormone (lhrh)-immunoreactive neurons are found in a cell group (nucleus olfactoretinalis) located at the transition between the ventral telencephalon and olfactory bulb. processes of these neurons project to the contralateral retina, traveling along the border between the internal plexiform and internal nuclear layer, and probably terminating on amacrine or bipolar cells. horseradish peroxidase (hrp) injected into the eye ... | 1982 | 7044556 |
| ultrastructural visualization of galactose in the glycoprotein of gastric surface cells with a peanut lectin conjugate. | the ultrastructural localization of peanut lectin-binding sites in the gastric surface epithelial cell has been studied using a horseradish peroxidase-labelled peanut lectin (pl-hrp) conjugate and other cytochemical techniques. the pl-hrp procedure has visualized glycoprotein with presumed terminal galactose residues in the apical plasmalemma and secreted mucins and has localized such glycoprotein selectively in the 'intermediate golgi cisternae' situated between the saccules of the maturing fac ... | 1982 | 7037694 |
| optical sectioning of hrp-stained molluscan neurons. | the use of high-resolution differential interference contrast(dic) microscopy on cleared whole-mounts of the circumesophageal nervous system from hermissenda crassicornis permits visualization of neuronal morphology in detail without the need for physical sectioning. such optical sectioning, when preceded by intracellular iontophoresis of horseradish peroxidase (hrp) permits rapid and accurate examination of the arborization of electrically characterized neurons. details such as varicosities and ... | 1982 | 7035759 |
| quantitative evaluation of the number of fibers in the right and left fasciculus retroflexus using horseradish peroxidase. | a quantitative evaluation of the number of fibers in the right and left fasciculus retroflexus (fr) was conducted in the frog rana esculenta after injections of horseradish peroxidase (hrp) in the habenular nuclei of the two sides of the brain and in the interpeduncular area in separate animals. it seems that the known morphological asymmetry of the habenular nuclei is not accompanied by a corresponding asymmetry of the fr and that there is an equivalent number of fibers in the fr after hrp inje ... | 1982 | 6984806 |
| the connections of the frog interpeduncular nucleus (itp) demonstrated by horseradish peroxidase (hrp). | | 1982 | 6978264 |
| effects of ascorbate on normal and abnormal leucocyte functions. | the stimulatory effects of ascorbate on neutrophil motility in vitro and in vivo and lymphocyte transformation to mitogens following ingestion or intravenous injection of ascorbate have been found to be related entirely to inhibition of the autooxidative effect of the myeloperoxidase/hydrogen peroxide/halide system (mpo/h2o2/halide system). stimulation of neutrophil migration and lymphocyte transformation following a single intravenous injection of 1 g of ascorbate was associated with inhibition ... | 1982 | 6811483 |
| double labeled-antigen method for demonstration of intracellular antigens in paraffin-embedded tissues. | a double "labeled-antigen" method has been developed for the simultaneous staining of both kappa and lambda light chains in fixed paraffin sections. the method is a two step procedure utilizing a mixture of antisera against kappa and lambda light chains in the first stage, followed by the addition of a mixture of kappa antigen labeled with horseradish peroxidase and lambda antigen labeled with alkaline phosphatase. the selection of substrates yielding reaction products of contrasting color enabl ... | 1982 | 6798106 |
| vascular and tubular renin in the kidneys of mice. | monospecific antisera and the immunocytochemical pap-method have been used to localize renin in the kidneys of mice. with this procedure, reaction product was not only observed in the epitheloid cells of kidney vessels but also in kidney tubules: in the apical portion of proximal tubule cells and in some cells of the connecting and the cortical collecting tubule. to answer the question, whether the occurrence of renin in kidney tubule cells is the consequence of tubular synthesis or that of glom ... | 1982 | 6757200 |
| alterations in the proximal nephron of beige mice with the chédiak-higashi syndrome. | the proximal nephron of c57 beige mice with a genetic defect analagous to the chédiak-higashi syndrome (chs) has been compared with that of normal c57 black mice. the concanavalin a-horseradish peroxidase (con a-hrp) technique stained the brush border of the proximal straight tubule heavily in black mice and weakly in beige mice. in beige mice this method stained the brush border of the proximal convoluted tubules weakly and the brush border of the proximal straight tubules only negligibly. peri ... | 1982 | 6275710 |
| substance p-like immunoreactivity in the parietal eye visual system of the lizard uta stansburiana. | we examined the parietal eye visual system of the iguanid lizard uta stansburiana for the presence of substance p-like immunoreactivity by use of both immunofluorescence and peroxidase-antiperoxidase techniques. in the parietal eye no substance p-containing somata were found; however, its plexiform layer contained small (ca. 1 micron diam) immunoreactive fibers. these fibers apparently originate outside the parietal eye. immunoreactive fibers also were found in the parietal nerve, the dorsal sac ... | 1982 | 6185229 |
| dermatitis herpetiformis with linear iga deposition: ultrastructural localization of in vivo bound iga. | ultrastructural localization of bound iga in the skin of a patient suffering from dermatitis herpetiformis with linear iga deposition at the dermo-epidermal junction was studied using the horseradish peroxidase labelled antibody method. the reaction products were observed as electron-dense materials, not only immediately below the cytomembrane of the basal cells but also in the uppermost dermis just below the basal lamina. the major portion of the lamina lucida was free of the reaction products. ... | 1982 | 6183889 |
| efferent neurons of the lateral-line system and the viii cranial nerve in the brainstem of anurans. a comparative study using retrograde tracer methods. | the octavo-lateral efferent system of several anuran species was studied by means of retrograde transport of horseradish peroxidase. this system is organized similarly in all larval anurans and in all adult aglossids. all have two groups of efferent neurons in the nucleus reticularis medialis between the viiith and the ixth motor nucleus. the caudal group consists of efferent neurons that supply the posterior lateral-line nerve (nllp) and a considerably smaller group of neurons supplying both th ... | 1982 | 6181890 |
| transneuronal uptake of horseradish peroxidase in the central nervous system of dipterous insects. | horseradish peroxidase (hrp) applied to lesioned neurons in the retina and thoracic ganglia of the flies musca, calliphora and drosophila labeled axons terminals, dendrites and perikarya of the severed neurons after anterograde or retrograde passage. in addition, hrp reaction product secondarily labeled intact neurons that are contiguous with injured nerve cells. in many cases labeling of optic lobe neurons remote from primarily filled ones was also seen (here called "tertiary" labeling). hrp la ... | 1982 | 6181889 |
| inhibition by glucocorticoids of endocytosis in a macrophage-like cell line. | a macrophage-like cell line (p388d1) has been used to demonstrate that glucocorticoids inhibit the fluid-phase endocytosis of fluorescein-labeled dextran (fitc-dextran). initial experiments demonstrated that the interaction of fitc-dextran with cells had all the features of fluid-phase uptake, ie, the amount taken up was proportional to the concentration in the medium, the uptake proceeded continuously with time and was blocked at 4 degrees c. dexamethasone (10(-7) m) had no effect on endocytosi ... | 1982 | 6177708 |
| synaptic vesicle recycling at the neuromuscular junction in the presence of a presynaptic membrane marker. | staining of the presynaptic axonal membrane of the neuromuscular junction with horseradish peroxidase-labeled alpha-bungarotoxin was utilized as a marker for observing directly the fate of this membrane during the process of synaptic vesicle release and recycling. the neuromuscular junctions of frog sartorius-sciatic nerve preparations were stained with horseradish peroxidase-alpha-bungarotoxin and stimulated by electrical stimulation of the nerve, high concentration of external potassium ions, ... | 1982 | 6176905 |
| microanatomy of the subumbrellar motor innervation in aglantha digitale (hydromedusae: trachylina). | the hydrozoan medusa aglantha digitale (müller 1776) has eight syncytical giant motor axons, up to 40 micrometers in diameter, running from the margin, up the inside of the bell towards the apex. giant motor axons injected with lucifer yellow ch are connected with lateral neurons running circumferentially across the subumbrellar muscle. these processes fill with the dye. bundles of 20 to 50 small dye-coupled neurons extend circumferentially along the margin for up to 0.85 mm. giant motor axons i ... | 1982 | 6121630 |
| anatomy of respiratory rhythmic systems in brain stem and cerebellum of the carp. | the afferent and efferent connections of two respiratory rhythmic loci in the dorsal mesencephalic tegmentum were studied by retrograde and anterograde transport of horseradish peroxidase. the injection areas were determined with extracellular activity recording using hrp filled glass micropipettes, and then followed by electrical stimulation and subsequent iontophoretic hrp delivery. one area in the nucleus of the posterior commissure was found to be optic related respiratory in nature and poss ... | 1981 | 7317791 |
| reversible microwave effects on the blood-brain barrier. | low level microwave exposure of chinese hamsters resulted in reversible permeability of the blood-brain barrier (bbb) to horseradish peroxidase (hrp). lesions were grossly visible in random areas of the brain immediately following exposure, but were not as common following a 1 h recovery period and were absent after a 2 h recovery period. the apparent route of increased permeability was via endothelial vesicular transport, since reaction product was not seen passing through the endothelial tight ... | 1981 | 7317776 |
| quenching of enzyme-generated acetone phosphorescence by indole compounds: stereospecific effects of d- and l-tryptophan. photochemical-like effects. | indole compounds efficiently quench the acetone phosphorescence observed during the horseradish peroxidase catalyzed aerobic oxidation of isobutyraldehyde. different types of stern-volmer plots are observed: linear, linear with two slopes, and downward- and upward-curved plots. the complexity probably stems from the operation of both dynamic and static quenching, coupled with different efficiencies of quenching of various acetone triplet populations. binding to the enzyme may also occur, especia ... | 1981 | 7326227 |
| plasticity in a central nervous pathway in xenopus: anatomical changes in the isthmotectal projection after larval eye rotation. | in this paper we present evidence that early eye rotation in xenopus leads to anatomical rearrangements in a portion of the binocular visual system. in the past, electrophysiological mapping had shown that the topography of the ipsilateral visuotectal projection is changed by such eye rotation and that this change requires visual experience. however, knowledge of the anatomical basis for this electrophysiological change was lacking. the identification of the nucleus isthmi as a link in the proje ... | 1981 | 7328201 |
| the accessory optic system of rana pipiens: neuroanatomical connections and intrinsic organization. | the accessory optic system of rana pipiens was investigated by autoradiographic, horseradish peroxidase, and golgi techniques, revealing a complexity of neuroanatomical organization previously unrecognized. retinal afferents project to the nucleus of the basal optic root (nbor) via a primary bundle and more diffuse, medial bundle of optic axons, both of which contain large- and small-diameter fibers. at least six types of retinal ganglion cell contribute to the basal optic root (bor), including ... | 1981 | 7035505 |
| the protoporphyrin-apoperoxidase complex as a horseradish peroxidase analog. a fluorimetric study of the heme pocket. | similarity of the protein tertiary structures of the native horseradish peroxidase (donor: hydrogen-peroxide oxidoreductase, ec 1.11.1.7) and protoporphyrin-apoperoxidase complex has been shown on the basis of identity of the tryptophan fluorescence parameter at ph 2.0-8.0 and of the circular dichroism spectra of the two proteins. absorption and fluorescence spectra have been obtained for protoporphyrin in the complex in the ph range 7.0-1.6. a shift in the apparent pk by 4 units has been observ ... | 1981 | 7317437 |
| the origin of centrifugal fibers to the inner ear in caiman crocodilus. a horseradish peroxidase study. | the origin of acoustic and vestibular efferent fibers was investigated in caiman crocodilus. after injection of horseradish peroxidase (hrp) into the cochlear duct or into the ampullae of the horizontal and anterior semi-circular canals, cells in the medulla oblongata exhibited retrogradely transported hrp reaction product. efferent vestibular fibers were found in the medial reticular nucleus, at the level of the oliva superior. there were more labeled neurons ipsilateral to the injection site. ... | 1981 | 6172755 |
| identification and localization of the motor nuclei and sensory projections of the glossopharyngeal, vagus, and hypoglossal nerves of the cockatoo (cacatua roseicapilla), cacatuidae. | horseradish peroxidase (hrp) has been applied to the proximal severed ends of glossopharyngeal (n ix), vagus (nx), and hypoglossal (n xii) cockatoo in order to localize the motoneurons and sensory projections of these nerves which are involved in the control of the bird's feeding and phonatory behaviors. application of hrp to n ix labeled four rhombencephalic nuclei: (1) a large-celled, retrofacial nucleus supplying m. geniohyoideus, the major tongue extensor; (2) a dorsal nucleus composed of me ... | 1981 | 6274918 |
| pathway selection by embryonic chick motoneurons in an experimentally altered environment. | to characterize cues used by motoneuron axons to reach their appropriate targets, connectivity patterns within the embryonic chick hindlimb have been analysed after early experimental manipulations of the limb or spinal cord. the manipulations altered the anterior-posterior (a.-p.) relationship between motoneurons within the lumbosacral motor column and their specific targets in the limb. primary emphasis was placed on analysing the pathways taken by embryonic motoneuron axons at stages 23-36 wh ... | 1981 | 6121329 |
| pathway selection by chick lumbosacral motoneurons during normal development. | pathways taken by motoneuron axons from the lumbosacral lateral motor column to individual hindlimb muscles have been characterized throughout the normal period of outgrowth and the establishment of specific functional connections in the chick embryo. axon pathways from individual cord segments were identified after injections of horseradish peroxidase (hrp) directly into the cord. labelled motoneuron axons were then traced through the plexus and major nerve trunks to termination sites within th ... | 1981 | 6121327 |
| altered phospholipid composition affects endocytosis in cultured lm fibroblasts. | the phospholipid polar head group composition of lm fibroblast membranes was altered by growing the cells in a chemically defined, serum-free medium containing choline, n,n'-dimethylethanolamine, n-monomethylethanolamine, or ethanolamine. the cells incorporated these bases into their membrane phospholipid such that 29-40% of the total plasma membrane phospholipids contained these polar head groups. alteration of the phospholipid composition correlated with a depression of polystyrene bead phagoc ... | 1981 | 7317390 |
| a glial blood-brain barrier in elasmobranchs. | the blood-brain interface in elasmobranchs was examined using physiological and anatomical techniques. electrolyte analyses of plasma and cerebrospinal fluid (csf) in control fish and in fish with elevated plasma cation concentration (ca, mg or k) demonstrated homeostasis of csf calcium and magnesium concentrations in dogfish and skates and of csf potassium concentration in dogfish. in contrast, csf potassium concentration was not regulated independent of plasma potassium concentration in skates ... | 1981 | 7296300 |
| the interaction of benzhydroxamic acid with horseradish peroxidase and its fluorescent analogs. | | 1981 | 7325673 |
| quantitative data on peroxidatic markers for electron microscopy. with a note on actin identification in paramecium cells. | several important points of heme-peptide cytochemistry were quantitatively analyzed, with particular regard to their use in electron microscopic immunocytochemistry. a simple procedure is presented for the preparation of heme-octapeptide (h-8-p) microperoxidase. h-8-p, hemenonapeptide (h-9-p), and various horseradish peroxidase (hrp) isoenzymes were used for coupling with immunoglobulin (ig)g or the papain-cleavage fragments from igg (fab) molecules. ultracentrifugation and spectrophotometric an ... | 1981 | 7033365 |
| multiple classes of photoreceptors and neurons in the frontal organ of rana pipiens. | an attempt has been made to characterize the photoreceptors and neurons of the frontal organ of rana pipiens and the synaptic contacts of these cells, in order to provide morphological correlates for previous physiological findings. neurons, glial cells, and photoreceptors with two different appearances can be seen in normal anatomical preparations. the photoreceptors make ribbon synaptic contacts onto both neurons and other photoreceptors. retrograde transport of either cobalt or horseradish pe ... | 1981 | 6975783 |
| a new device for making multiple injections of small volumes of horseradish peroxidase into the spinal cord. | a device which allows multiple injections of horseradish peroxidase to be made into the spinal cord with minimal tissue damage is described. the unit is constructed from commercially available capillary glass, silicon vacuum grease and fast drying dental cement. it is cheap and simple to construct and permits the injection of known volumes as small as 7.8 nl. | 1981 | 6172675 |
| efferents to the retina have multiple sources in teleost fish. | multiple efferent systems project to the retina in three species of teleost fish investigated with the horseradish peroxidase technique. these animals are the first vertebrates shown to have more than one central nervous system structure projecting to the retina. the connections discovered may reflect a primitive organization of retina-brain interconnections. | 1981 | 6171033 |
| topographic projections between the nucleus isthmi and the optic tectum in a teleost. navodon modestus. | following horseradish peroxidase injections into the optic tectum of a teleost, navodon modestus, reciprocal and topographic projections between the nucleus isthmi and the ipsilateral optic tectum were determined. the isthmo-tectal fibers diverge to the optic tectum while maintaining the spatial arrangements of the isthmic cells from which the fibers originate. the tecto-isthmic projections also keep the spatial arrangements in the optic tectum. the tectal fibers converge near the nucleus isthmi ... | 1981 | 6169409 |
| horseradish peroxidase c. | horseradish peroxidase c (hrp; ferric) reacts with h2o2 to form compound i, with an equilibrium constant of about 10(14) m-1. two-step reduction of compound i to compound ii and further to the ferric enzyme occurs reversibly at eo' values of 0.90 and 0.93 v (ph 7.0), respectively. the ph dependence of eo' values for each one-electron step, ferrous leads to ferric leads to compound ii leads to compound i indicates that presence of redox-linked ionization at pka values of 7.3 in the ferrous state, ... | 1981 | 7322114 |
| purification and properties of bromoperoxidase from penicillus capitatus. | a bromoperoxidase has been isolated and purified from the marine green algae, penicillus capitatus. the purified enzyme is homogenous as determined by polyacrylamide gel electrophoresis and ultracentrifugation. bromoperoxidase can utilize bromide ions in the presence of hydrogen peroxide and a halogen acceptor for the catalytic formation of carbon-halogen bonds. based on equilibrium ultracentrifugation results the molecular weight of bromoperoxidase is 97,600. sodium dodecyl sulfate polyacrylami ... | 1981 | 7287765 |
| efferent neurons to the labyrinth of salamandra salamandra as revealed by retrograde transport of horseradish peroxidase. | application of horseradish peroxidase to the severed viiith nerve of salamandra salamandra resulted in heavy bilateral labeling of neurons of the medullary reticular formation. these neurons closely resemble the mauthner neuron in their widespread dendritic ramification. in most preparations axon collaterals are seen to leave the medulla via the contralateral viiith nerve. it is suggested that these neurons are labyrinthine efferents. | 1981 | 7322432 |
| locus coeruleus neurons have axons that branch to the forebrain and cerebellum. | utilizing a recently introduced double retrograde axonal tracing technique, the existence of collateralized axonal projections of locus coeruleus neurons to both forebrain and cerebellar cortical structures has been examined in adult mice. following paired injection strategies using horseradish peroxidase and radiolabeled wheat germ agglutinin within portions of the forebrain and cerebellar cortices, substantial numbers of double labeled 'branched' neurons have been found that reside within all ... | 1981 | 6169404 |
| morphology and distribution of touch cell terminals in the skin of the leech. | 1. the receptor terminals of individual mechanosensory neurones responding to light touch (t cells) have been visualized directly in the skin of the leech by injecting horseradish peroxidase (hrp) into their cell bodies in the central nervous system. the axons of injected cells could be followed from their origin in the neuropile of the ganglion to their terminals in the skin. 2. the axons of t cells run through ipsilateral nerve roots in the body wall to the base of the layer of epithelial cell ... | 1981 | 7320936 |
| [effect of temperature and composition of frozen solutions on the rate of peroxidase oxidation of 2,3-dimethyl-1,4-naphthoquinol-1-dimethylphosphate]. | the horseradish peroxidase-catalyzed oxidation of 2.3-dimethyl-1.4-naphthoquinol-1-dimethylphosphate by hydrogen peroxide was investigated within the temperature range of +20 divided by -35 degrees. the increase in the concentration of the buffer solution accelerated the reaction in liquid solutions at 0 degrees and 20 degrees. the unusual shapes of the curves of temperature dependences of the reaction rate in frozen solutions of different composition can probably be due to temperature variation ... | 1981 | 7317533 |
| rules of motor innervation in chick embryos with supernumerary limbs. | the positions of motoneurons supplying individual muscles in chick embryos with grafted supernumerary limbs have been identified using retrograde transport of horseradish peroxidase. for a given muscle, motor pool location varied depending on the embryonic origin of the muscle, the position of the limb along the rostrocaudal axis of the body, and the limb's orientation with respect to the body wall. limb muscles derived from the ventral part of the embryonic premuscle mass were always innervated ... | 1981 | 7298909 |
| an elisa method for detecting unexpected antibodies. | this paper reports a new horseradish peroxidase enzyme linked immunosorbant assay (elisa) to detect patient serum alloantibodies. the new methodology utilizes a water-soluble chromogen, o-dianisidine and a temporary solid antibody support, osmotically lysed reagent erythrocytes. in a double blind study the horseradish peroxidase elisa was found to be as sensitive as the conventional antiglobulin test. in parallel dilution studies on serum samples containing anti-kell antibodies, the horseradish ... | 1981 | 7032295 |
| direct electrical connections between transient amacrine cells in the catfish retina. | transient amacrine cells were identified by their intracellularly recorded response to flashes of light. these cells typically respond with a transient depolarization, often followed by a steady-state response during the stimulus. when two electrodes were placed in different transient amacrine cells, current of either polarity passed through one electrode produced a steady-state voltage change that was recorded by the electrode in the nearby cell. following identification of the physiological ty ... | 1981 | 7291987 |
| origin of the parallel fibers in the cerebellar crest overlying the intermediate nucleus of the elasmobranch hindbrain. | this study utilizes anterograde transport of horseradish peroxidase (hrp), golgi staining, and electrophysiological techniques to demonstrate the origin of the parallel fibers which constitute the molecular layer overlying the intermediate nucleus of the dogfish hindbrain. the parallel fibers are shown to arise from granule cells of the auricle. the majority come from the granular area of the lower leaf of the auricles, which is termed the lateral granule cell area of the auricle. a band of para ... | 1981 | 7298897 |
| long-term survival of centrally projecting axons in the optic nerve of the frog following destruction of the retina. | a significant number of unmyelinated axons and their synaptic endings in the frog, rana pipiens, were found to retain a normal morphology long after separation from their cell bodies. at the end of various survival periods following unilateral removal of the retina, horseradish peroxidase (hrp) was administered to the optic nerve stump by a fiber-filling method. in frogs maintained at 20 degrees c, unmyelinated optic nerve axons conducted hrp from the site of application in the orbit to layers a ... | 1981 | 6974743 |
| brainstem afferents to the torus semicircularis of the queensland cane toad (bufo marinus). | the ascending projections to the major midbrain auditory center of anura, the torus semicircularis, have been examined using the technique of retrograde labeling with horseradish peroxidase (hrp). localized iontophoretic injections of hrp were made in one torus semicircularis of queensland cane toads (bufo marinus). visualization of hrp-labeled neurons with tetramethyl benzidine revealed that the torus semicircularis receives its major afferent input from the ipsilateral superior olive. other pr ... | 1981 | 6793645 |
| horseradish peroxidase. complex formation with anions and hydrocyanic acid. | equilibrium binding experiments have been performed with perchlorate, chloride, and acetate in the presence of horseradish peroxidase. the binding of perchlorate and acetate appears to be like that of nitrate, at a site other than the sixth coordination position of the heme iron. competitive experiments using both nitrate and cyanide demonstrate that two different binding sites are present on the enzyme. chloride appears to bind at the sixth coordination position as do both fluoride and cyanide. ... | 1981 | 6268626 |
| a retinopetal nucleus in the preoptic area in a teleost, navodon modestus. | following horseradish peroxidase (hrp) applications to the optic nerve of a teleost (navodon modestus), a retinopetal nucleus was identified in the contralateral preoptic area. the nucleus was composed of small (7-10 microns) round cells. centrifugal fibers from the nucleus were traced to the inner nuclear layer of the ipsilateral retina by both orthograde hrp and fink-heimer methods. the cells in the nucleus showed no neurosecretion. the retinopetal nucleus or neurons could not be found in cara ... | 1981 | 6170383 |
| selective bilateral motor innervation in xenopus tadpoles with one hind limb. | bilateral innervation of a single hindlimb bud was induced by amputating the other limb bud and disrupting the barriers between the two sides. though the routes of the crossed nerves were necessarily abnormal, the motor projections that developed subsequently were normal as determined by horseradish peroxidase tracing. the limb therefore appears to be innervated selectively, each region being invaded and/or synapsed with only by motoneurones at particular locations. the numbers of motoneurones s ... | 1981 | 7334297 |
| [distribution of the afferent elements in the central nervous system of the pond snail (lymnaea stagnalis l.)]. | to study distribution of afferent fibres of all nerves of the cerebral ganglia in the central nervous system (cns) of the pond snail lymnaea stagnalis, cocl2 and horseradish peroxidase solutions were administered. in the cns certain neurons were revealed which could be considered as primary sensitive according to some signs. it has been stated that the sensitive fibres are situated in neuropil as well formed, definitely localized fasciculi. most of them terminate immediately in the cerebral gang ... | 1981 | 7325811 |
| effect of modification of heme propionate groups on the reactivity of horseradish peroxidase. | | 1981 | 7305374 |
| retinal afferents and efferents of an infrared sensitive snake, crotalus viridis. | the retinal afferents and efferents were examined in crotalus viridis. retinofugal fibers were traced by injecting horseradish peroxidase (hrp) or tritiated leucine into the eye, or by removing the eye and staining degenerating axons with silver methods. terminations were seen contralaterally in the suprachiasmatic nucleus, the dorsal and ventral lateral geniculate nuclei (extensive), the pretectal nuclei, including the nucleus posterodorsalis (a very heavy input), the nucleus lentiformis mesenc ... | 1981 | 7288885 |
| effects of pressure and temperature on the reactions of horseradish peroxidase with hydrogen cyanide and hydrogen peroxide. | reactions of ferric horseradish peroxidase with hydrogen cyanide and hydrogen peroxide were studied as a function of pressure. activation volumes are small and differ in sign (delta v = 1.7 +/- 0.5 ml/mol for peroxidase + hcn and -1.5 +/- 0.5 ml/mol for peroxidase + h2o2). the temperature dependence of cyanide binding to horseradish peroxidase was also determined. a comparison is made of relevant parameters for cyanide binding and compound i formation. | 1981 | 6269668 |
| the laminar origin and distribution of the crossed tectoreticular pathways. | the superior colliculus is the source of a prominent descending pathway which crosses the midline in the mesencephalon and projects to the paramedian pontine reticular formation. the primary goal of the present study was to identify the cells in the superior colliculus of the grey squirrel which give rise to this pathway by using a combination of anterograde and retrograde tracing techniques. results from the anterograde studies demonstrated the course and terminal distribution of this pathway a ... | 1981 | 6169813 |
| vestibular efferent neurons and catecholamine cell groups in the reticular formation of the pigeon. | this study was designed to investigate the relationship between vestibular efferent neurons and catecholamine cell groups within the reticular formation of the pigeon. horseradish peroxidase (hrp) was injected into the endolymphatic space of one labyrinth. the brain was perfused with glyoxylic acid and serial sections were examined with a fluorescence microscope. after documentation of the location of fluorescent cells the sections were reacted for hrp with tetramethylbenzidine. fluorescent cell ... | 1981 | 6974836 |
| nucleus laminaris of the torus semicircularis: projection to spinal cord in reptiles. | neurons in nucleus laminaris of the torus semicircularis were retrogradely labeled following application of horseradish peroxidase (hrp) to the cervical spinal cord in two lizards (gekko gecko and iguana iguana) and a turtle (pseudemys scripta). different patterns of cell labeling were seen among the species studied and may be related to the distinctive differences in head and body movements seen in these animals during defensive, aggressive and social behaviors. | 1981 | 6270598 |
| lhrh systems in brain of platyfish. | the luteinizing hormone-releasing hormone (lhrh) system of the platyfish xiphophorus has been studied using immunohistochemistry and retrograde transport of horseradish peroxidase (hrp). three different populations of lhrh-positive cell bodies are present in the brain, one in the ventral telencephalon at the border to the olfactory bulb (nucleus of olfactoretinalis), one lateral to the n. preopticus (nucleus preopticus basalis lateralis) and one in the midbrain. lhrh neurons from the nucleus olf ... | 1981 | 7023610 |
| auditory responses in the zebra finch's motor system for song. | vocal learning in songbirds is critically dependent on auditory information. intracellular electrophysiological recordings, combined with horseradish peroxidase staining of single cells has revealed neurons within one central nervous system vocal control nucleus, hyperstriatum ventrale, pars caudale (hvc) that show responses to auditory stimuli. auditory and non-auditory neurons fall into distinct morphological classes, based on soma size, dendritic field structure, and efferent projections. the ... | 1981 | 6168335 |
| delayed luminescence of luminol initiated by a membrane-bound peroxidase. | the luminescense of the luminol-h2o2 system was initiated by either free or membrane-bound horseradish peroxideae (hrp). the instantaneous luminescene decayed rapidly and was followed by the delayed luminescence in the presence of excess luminol. the delayed luminescence was characterized by a chain reaction, in which luminescence intensity increased exponentially. membrane-bound hrp demonstrated that the delayed luminescence took place even in the absence of hrp if the instantaneous luminescenc ... | 1981 | 24233883 |
| physiological and anatomical evidence for a discontinuous representation of the trunk in s-i of tree shrews. | microelectrode mapping methods revealed that the representation of the body surface in the first somatosensory area of cortex, s-i, of the tree shrew is unique in that only the ventral trunk was found in the usual location of the trunk representation in cortex of the dorsolateral surface of the cerebral hemisphere. instead, the dorsal trunk was found as an extension of the representation of the posterior leg in cortex on the medial wall. the separation of the representation of the trunk occurs a ... | 1981 | 7276249 |
| organization of somatosensory input to the deep collicular laminae in hamster. | retrograde transport of horseradish peroxidase (hrp) was used to delineate the sources of somatosensory input to the hamster's superior colliculus. cells in the ipsilateral somatosensory cortex and contralateral dorsal horn of the spinal cord, dorsal column nuclei, lateral cervical nucleus, internal basilar nucleus, nucleus of the spinal trigeminal tract and deep layers of the superior colliculus were labeled following hrp injections centered in the deep tectal laminae. the response characterist ... | 1981 | 7271988 |
| [kinetics of peroxidase recombination with carbon dioxide in the +20 to -70 degree c temperature interval]. | using flash photolysis, horseradish peroxidase rebinding of carbon monoxide has been observed in the temperature range between 20 and -70 degrees c. a lag-period of about 50 ms immediately after photodissociation takes place. this lag-period is assumed to be due to the formation of the intermediate protein form with the low reactivity. following rebinding is bimolecular process with the rate constant 4 x 10(3) m-1 s-1 usually observed for the peroxidase reaction with carbon monoxide. for some pe ... | 2013 | 6797479 |
| [stimulating effect of focused ultrasound on auditory fibers of the acoustic nerve in the frog rana temporaria]. | after mechanical destruction of the receptor apparatus, application of focused ultrasound (frequency 2.34 mhz) to the auditory fibers of the frog elicited the electrical activity in the auditory midbrain centers (torus semicircularis). action potentials evoked by focused ultrasound were similar to those evoked by activation of the intact contralateral labyrinth. after introduction of horseradish peroxidase into the destroyed auditory capsule, fibers activated by ultrasound were detected. therefo ... | 2006 | 6170194 |
| receptor-mediated endocytosis in cultured fibroblasts: cryptic coated pits and the formation of receptosomes. | concentrative receptor-mediated endocytosis of many specific ligands by cultured fibroblasts occurs through the coated pit-receptosome pathway. the formation of receptosomes was studied using two impermeant electron-dense labels for the cell surface, ruthenium red and concanavalin a-horseradish peroxidase. these studies show that at 4 degrees c, virtually all coated structures near the plasma membrane are in communication with the cell surface, and are not isolated coated vesicles. on warming ce ... | 1981 | 6169759 |
| axonal branching pattern and coupling mechanisms of the cerebral giant neurones in the snail, lymnaea stagnalis. | the axonal branching pattern of the two cerebral giant neurones (cgcs) of lymnaea stagnalis was studied with intrasomatically applied horseradish peroxidase. the cells are symmetrical. each cgc projects to the ipsilateral n. labialis medius and n. arteriae labialis, the subcerebral commissure, and to all ipsi- and contralateral buccal nerves. the contralateral buccal nerves are reached via the ipsilateral cerebro-buccal connective and the buccal commissure. the cgc fire action potentials 1:1 in ... | 1981 | 6168740 |
| [somatotopic relations between the olfactory mucosa and the olfactory bulb in the triton]. | the axons of receptors located in the olfactory neuroepithelium are known to synapse with mitral cell dendrites in the glomerular layer of the ipsilateral olfactory bulb, but few previous studies showed a clear-cut topological organization of this projection. we therefore injected horseradish peroxidase (hrp) into restricted areas of the olfactory mucosa in adult tritons, triturus cristatus. the animals were perfused with glutaraldehyde 17 h later; the mucosa, the olfactory nerve and bulb were r ... | 1981 | 7260633 |
| fluctuating responses at a central synapse: n of binomial fit predicts number of stained presynaptic boutons. | binomial predictions provided a better description than the poisson law of fluctuating unitary inhibitory postsynaptic potentials evoked in the goldfish mauthner cell by impulses in presynaptic interneurons. the number of terminal boutons established on this target cell by each horseradish peroxidase-filled interneuron corresponded to the value of the binomial parameter n. | 1981 | 6266015 |
| ascending projections of the brain stem reticular formation in a nonmammalian vertebrate (the lizard varanus exanthematicus), with notes on the afferent connections of the forebrain. | in the present study an attempt has been made to analyze the ascending reticular projections in the lizard varanus exanthematicus by means of the horseradish peroxidase (hrp) technique. reticular projections ascending to the telencephalon were found to arise in the mesencephalon, but not caudal to the mesorhombencephalic border. hrp injections into the dorsal thalamus have demonstrated retrogradely labeled cells in the mesencephalic reticular formation, particularly at the level of the oculomoto ... | 1981 | 7263959 |
| the use of horseradish peroxidase-lectin conjugates to monitor the purity of plasma-membrane preparations obtained from isolated cells. | the plasma membranes of viable murine el4 tumour cells were labelled with horseradish peroxidase-conjugated wheat-germ agglutinin. after disruption of the labelled intact cells, plasma-membrane purification could be monitored by ultrastructural examination of the various fractions for positive reaction product on the membrane vesicles. | 1981 | 7326013 |
| long-term survival of glial segments during nerve regeneration in the leech. | nerve injury that severs axons also disrupts ensheathing glial cells. specifically, crushing or cutting the leech nerve cord separates the glial cell's nucleated portion from an anucleate recording, by intracellular injection of lucifer yellow dye and horseradish peroxidase (hrp) as tracers, and by electron microscopy. the nucleated portion of the glial cell did not divide, degenerate, or grow appreciably. the severed glial stump remained isolated from the nucleated portion but maintained its re ... | 1981 | 7023608 |
| rohon-beard neurons arise from a substitute ancestral cell after removal of the cell from which they normally arise in the 16-cell frog embryo. | in 16-cell xenopus embryos, horseradish peroxidase (hrp) was injected into blastomere d1.2 on one side. no rohon-beard neurons originated from d1.2 in either of the two patterns of cleavage that were studied (jacobson, m. (1981) j. neurosci. 1: 918-922). in other embryos, after injection of hrp into d1.2, the neighboring ventral blastomere v1.2, from which 68 to 90% of rohon-beard neurons normally originate, was removed. in the cases that developed normally to larval stages 32 to 34, the number ... | 1981 | 7346596 |
| rohon-beard neuron origin from blastomeres of the 16-cell frog embryo. | clonal origins of rohon-beard neurons in xenopus were determined quantitatively by injecting horseradish peroxidase into individual blastomeres at the 16-cell stage and later counting labeled and unlabeled rohon-beard neurons. two different patterns of cleavage were selected. in pattern x, all rohon-beard neurons originated from three blastomeres (v1.1, v1.2, and v2.2) on each side; in pattern y, all rohon beard neurons originated from two blastomeres (v1.2 and v2.2) on each side. counts of roho ... | 1981 | 7346595 |
| the growth and organization of the optic nerve and tract in juvenile and adult goldfish. | the optic nerves, tracts, and tecta of goldfish, 1 to 5 years old, have been studied anatomically using light and electron microscopy, horseradish peroxidase (hrp), and tritiated proline radioautography. the aims were to document an earlier inference that fibers are added to the nerve continually and to describe the growth and organization of the pathway. (1) the numbers of optic fibers were counted in electron micrographs of the nerve. there were about 120,000, 165,000 and 180,000 in 1-, 3-, an ... | 1981 | 7346585 |
| isolation and properties of basic isoenzymes of horseradish peroxidase. | six basic isoenzymes, e1 to e6, of horseradish peroxidase were isolated and purified by cm-sephadex column chromatography, and were then crystallized. the crystalline basic isoenzymes had a lower molecular weights than the neutral isoenzyme c, but the molecular weights of their protein moieties were similar to that of the latter. the lower molecular weights of the basic isoenzymes were thus essentially due to smaller contents of carbohydrate. of the six isoenzymes, four isoenzymes, e3 to e6, had ... | 1981 | 7298597 |
| secondary vestibulocerebellar projections to flocculonodular lobe in a prosimian primate, galago senegalensis. | the organization of vestibulocerebellar projections to the flocculonodular and adjacent cortices were studied in galago using horseradish peroxidase (hrp). implants of hrp pellets or injections (0.14-0.95 microliter) were placed in floccular, parafloccular, nodular, and uvular cortices. following survival times of 18-25 hours animals were killed via transcardiac perfusion of heparinized saline followed by a buffered solution of paraformaldehyde and glutaraldehyde. tissues were processed using da ... | 1981 | 7287921 |
| projections from the superior colliculus and the neocortex to the pulvinar nucleus in galago. | we have studied the projections from the superior colliculus and the neocortex to the pulvinar nucleus in galago senegalensis by using the retrograde transport of horseradish peroxidase (hrp). injections of various parts of the pulvinar complex, both the inferior and superior divisions, both the tectorecipient zone and the nontectorecipient zone as defined by glendenning et. al. ('75), produce labeled cells in the lower tier of stratum griseum superficiale. the distribution of labeled cells in t ... | 1981 | 7287920 |
| a simple and inexpensive method for 3-dimensional visualization of neurons reconstructed from serial sections. | neurons stained by the intracellular injection of horseradish peroxidase or fluorescent dyes are often reconstructed from serial sections as a projection onto a 2-dimensional plane, with consequent loss of depth information. we have developed a simple, rapid and inexpensive method that retains depth information and permits visualization of neuronal structure in 3 dimensions, either as stereo pairs of red-green anaglyph drawings. the procedure does not require computer assistance and can be accom ... | 1981 | 7024652 |
| functional organization of the oculomotor nucleus in the baboon. | the functional organization of the oculomotor nucleus was investigated using horseradish peroxidase (hrp) histochemistry. in a series of baboons, injections of hrp were made into the skeletal muscles supplied by the oculomotor nerve (medial rectus, superior rectus, inferior rectus, inferior oblique, and the levator palpebrae superioris). after a 48-hour survival time the animals were sacrificed via perfusion-fixation and the brains treated according to the tetramethylbenzidine (tmb)-hrp method o ... | 1981 | 6269415 |
| mapping of neuronal contacts with intracellular injection of horseradish peroxidase and lucifer yellow in combination. | a technique for the simultaneous visualization in the light microscope of processes of neurons filled with horseradish peroxidase and lucifer yellow in combination has been developed. the technique is applied to determine the location, number and distribution of presumptive synaptic sites between neurons in the leech central nervous system. | 1981 | 7020878 |
| organization of thalamic afferents to anterior dorsal ventricular ridge in turtles. i. projections of thalamic nuclei. | dorsal ventricular ridge (dvr) is a thalamorecipient, subcortical telencephalic structure in reptiles and birds. although there is a fair amount of information about sources of afferents to dvr, little is known about the relationship of projections from individual thalamic nuclei to the organization of the structure. this study examines the relationship between thalamic projections and both areal and zonal divisions of anterior dvr (advr; balaban, '78a) of emydid turtles with orthograde degenera ... | 1981 | 7251947 |
| development of the pyramidal tract in the hamster. i. a light microscopic study. | the development of the pyramidal tract and other projections from the sensorimotor cortex was studied in the postnatal hamster with both (3h) proline and horseradish peroxidase (hrp) as anterograde tracers. in the 1-day-old animal labeled axons extend as far as the pons. other corticofugal fibers have penetrated into the corpus striatum and the thalamus. by 2 days postnatally, the pyramidal tract has grown to midmedullary levels and there is substantial retrograde (hrp) and anterograde labeling ... | 1981 | 7251945 |
| substituted hemins as probes for structure-function relationships in horseradish peroxidase. | low temperature visible spectra of compounds i from peroxidases reconstituted with protohemin, 2-formyl-4-vinyldeuterohemin, 2-vinyl-4-formyldeuterohemin, 2,4-dimethyldeuterohemin, and 2,4-diacetyldeuterohemin reveal that these fe(iv) porphyrin pi-cation radicals take the 2a2u of peroxidase-type electronic ground state. compound i of deuterohemin horseradish peroxidase, however, takes the 2a1u or catalase type pi-cation radical electronic ground state. since deuterohemin horseradish peroxidase p ... | 1981 | 7240251 |
| conduction of velocity groups in the optic nerve of the north american opossum (didelphis virginiana): retinal origins and central projections. | the axonal conduction velocity groups in the optic nerve of the north american opossum were analyzed electrophysiologically and related to soma size groups of ganglion cells in terms of their retinal origin and laterality of projection. on the basis of analysis of field potentials and single unit responses recorded at the optic disc, three velocity groups were identified (d1, d2, and d3) and estimated to have average conduction velocities of 12, 8, and 5 meters/second. from recordings of the fie ... | 1981 | 6168667 |
| the recognition of premalignant change in jejunal mucosal biopsies of patients with malabsorption. | the peroral small intestinal biopsies of 5 patients with chronic malabsorption leading to a fatal course were examined retrospectively for the presence of abnormal (? premalignant) cells. techniques employed for their identification included routine histology, immunofluorescence, immunohistochemistry (horseradish peroxidase) and electron microscopy. the biopsies of 3 other patients, 2 with known lymphoma and 1 with alpha chain disease were examined for any similarity in the ultrastructural appea ... | 1981 | 7334546 |
| arterial air embolism: structural effects on the gerbil brain. | air injection into the carotid artery of adult mongolian gerbils caused, within 10 minutes, multifocal brain lesions. the extracellular spaces were widened and neurons, oligodendrocytes and myelin sheaths remained unchanged. the "delayed" effects of air embolism (first seen after 3 h) were similar to those observed in gerbils after unilateral carotid ligation. the histologic alterations after 3 h consisted of astrocytic swelling and shrinkage/necrosis of neuronal soma. the observations reported ... | 2016 | 7314163 |
| [cooxidation of potassium ferrocyanide and o-dianisidine by hydrogen peroxide catalyzed by horseradish peroxidase. substrate-substrate activation]. | the kinetics of cooxidation of potassium ferrocyanide and o-dianisidine by hydrogen peroxide catalyzed by horseradish peroxidase were studied. the peroxidation of potassium ferrocyanide is activated by o-dianisidine. a scheme illustrating the direct involvement of the enzyme in substrate-substrate activation is proposed. a method for determination of the rate constants of the first electron transfer from o-dianisidine to peroxidase (i. e. reduction of peroxidase e1 to e2) and of the constants fo ... | 1981 | 7272350 |
| comparison of chromogens for the determination of horseradish peroxidase as a marker in enzyme immunoassay. | o-phenylenediamine, 2,2'-azino-di(3-ethylbenzthiazoline sulphonic acid-6) (abts), o-dianisidine and 4-aminoantipyrine were compared as chromogens for the determination of horseradish peroxidase. highest sensitivity in the determination of horseradish peroxidase-igg conjugates in dissolved form was obtained with o-phenylenediamine. when these conjugates were used in a two-site binding enzyme immunoassay for hepatitis b surface antigen (hbsag), the steepest calibration curve and the lowest detecti ... | 1981 | 7035603 |
| binding of peanut lectin to germinal-centre cells: a marker for b-cell subsets of follicular lymphoma? | the binding of horseradish-peroxidase-labelled peanut lectin (hrp-pnl) to cryostat sections of tonsil, lymphoma lymph nodes, reactive lymph nodes and miscellaneous tumours demonstrated that pnl binds selectively to lymphocytes in germinal centres. lymph nodes from 21 patients with non-hodgkin's lymphomas were phenotyped as cell suspensions for pnl binding, and the following surface markers: e rosetting, c3d, sig, ok markers of t-cell subsets, ig heavy-chain and light-chain classes. there was a p ... | 1981 | 6789856 |
| olfactory receptor cell staining using horseradish peroxidase. | iontophoretic injection of horseradish peroxidase into severed olfactory nerve fascicles has been used to stain salamander olfactory receptor cell somata, their associated nerves, and their axonal terminations in the glomerular layer of the olfactory bulb. this technique gives homogeneous, golgi-like staining of individually identifiable receptor cells and has permitted preliminary mapping of the topographical relationship between the loci of receptors in the olfactory mucosa and sites of their ... | 1981 | 6168214 |