| renal sodium handling after noradrenergic stimulation of the lateral hypothalamic area in rats. | 1. the participation of special nephron segments in the renal control of sodium handling after adrenergic stimulation was investigated by determining lithium clearance in groups of 5-12 male wistar rats (230-300 g) microinjected with noradrenaline into the lateral hypothalamic area (lha). 2. microinjection of noradrenaline (12.5 to 100.0 nmol/microliters) into the lha promoted a significant decrease in proximal sodium reabsorption (controls, 86.5 +/- 1.3; 12.5, 81.4 +/- 2.0; 25.0, 72.6 +/- 2.4; ... | 1992 | 1342841 |
| sex-related differences in cardiovascular responses to common carotid occlusion in conscious rats. | mean arterial pressure and heart rate were determined in conscious, unrestrained groups of 10 male, female and androgenized female wistar rats 20 s (early pressor response) and 1 min (late sustained response) after bilateral carotid artery occlusion. the early pressor response, which is of carotid reflex origin, was 40% greater in female than in male rats (45 +/- 2 vs 63 +/- 3 mmhg, respectively). the late sustained response, which is of central origin (probably ischemic), did not differ between ... | 1992 | 1342843 |
| life history studies of heterophyid trematodes in the neotropical region: ascocotyle (leighia) hadra sp. n. | the life cycle of ascocotyle (leighia) hadra n. sp. was experimentally reproduced, starting from cercariae from naturally infected littoridina parchappei, collected from los ranchos stream, near mercedes city, buenos aires province, argentina. metacercariae were found encysted in the liver and mesentery of experimentally and naturally infected fishes cnesterodon decemmaculatus and jenynsia lineata. adults were obtained experimentally in chicks and mice. the natural host is unknown. the new speci ... | 1992 | 1343669 |
| [bacillary necrosis in larvae of the bivalve euvola ziczac (linnaeus, 1758) caused by a pseudomonas sp]. | the incidence of bacteria as etiological agents of diseases in larvae of bivalve mollusks, is well documented in the literature. in this study, a series of test were performed to identify and estimate pathogenic level of marine bacteria isolated during an epizootic in a larval culture system of the tropical scallop euvola ziczac. such bacteria was identified as pseudomonas sp. belonging to the group alcaligenes. the bacterium produced not only lethal effects on the scallop larvae through infecti ... | 1992 | 1343735 |
| distribution of split 5.8s ribosomal rna in diptera. | although the 5.8s ribosomal rna (rrna) of most eucaryotes consists of 155-170 nucleotides, in two dipterous species the 5.8s rrna consists of two pieces, 123 and 30 nucleotides in length. the distribution of split 5.8s rrna was studied in other diptera and the most closely related order siphonaptera to learn the origin of split 5.8s rrna. four species of mosquitoes, culex tritaeniorhynchus, culex pipiens molestus, aedes albopictus, anopheles sp. (culicidae) had a single 5.8s rrna consisting of a ... | 1992 | 1343775 |
| [natural parasitism of buffaloes in botucatu, sp, brazil. iii. dynamics of gastrointestinal parasitism in cows and their calves]. | gastrointestinal parasitism of 24 buffalo cows before parturition, and post-parturition, their infection and that of their respective calves during the following 30 weeks were studied. willis, hoffmann and whenever possible, the modified gordon & whitlock techniques were used for fecal examinations. toxocara vitulorum eggs were the earliest forms encountered in calves feces, as follows: during the 1st week after birth, 58.33% of the calves were positive, and in the 4th week, 100% of these animal ... | 1992 | 1343798 |
| cotylophoron travassosi sp. n. (trematoda--paramphistomidae) from cattle. | a new species of the genus cotylophoron (trematoda--paramphistomidae) --cotylophoron travassosi sp. n.--is described. the measurements of the worm and its structures are compared with the valid known species. | 1992 | 1343800 |
| description of raillietia flechtmanni sp. n. (acari: gamasida). | raillietia flechtmanni sp. n. is described from the ear canal of its type host the domestic buffalo. the new species parasitizes cattle as well in brazil. | 1992 | 1343804 |
| identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium anabaena sp. pcc 7120. | a nuclease that could be recovered from the supernatant of cultures, as well as from cell-free extracts, of the cyanobacterium anabaena sp. pcc 7120 was identified as a 29 kda polypeptide by its ability to degrade dna after electrophoresis in dna-containing sds-polyacrylamide gels. some clones of a gene library of strain pcc 7120 established in escherichia coli were found to produce the 29 kda nuclease. the nuca gene encoding this nuclease was subcloned and sequenced. the deduced polypeptide, nu ... | 1992 | 1343821 |
| [control of schistosomiasis mansoni in an area of low transmission]. | the schistosomiasis is transmitted by biomphalaria tenagophila in our study area (pedro de toledo, são paulo, brazil). from 1980 to 1990 epidemiological surveys in a population of 4,000 inhabitants, has shown that: prevalences by kato-katz (kkt), immunofluorescence (ft) and intradermal (idt) techniques were 22.8%, 55.5% and 51.8%, respectively; intensity of infection was low, 58.5 eggs per gram of faeces (epg); there were no symptomatic cases; prevalences were higher in mates, children and rural ... | 1992 | 1343901 |
| identification of schistosome-infected snails by detecting schistosomal antigens and dna sequences. | cercarial shedding tests do not provide species identification of the schistosomes concerned and cannot detect prepatent schistosomal infections. we have demonstrated that both immunodetection by elisa of schistosomal antigens in snail hemolymph, and dot hybridization of snail extracts by a dna probe representing highly repeated sequences, proved suitable for detecting infected snails during prepatency as well as patency. a group-specific monoclonal antibody was found to be suitable for detectin ... | 1992 | 1343903 |
| intermediate hosts of schistosoma mansoni in brazil. | the brazilian planorbidical chart is slowly but progressively been increased by new data. distribution of vector species of schistosoma mansoni, according to paraense, 1986, may be thus resumed: biomphalaria glabrata--delimited by parallels 13 and 21 degrees s and meridians 39 and 45 degrees w, area of greater dominance (southeast bahia, oriental hal of minas gerais and espírito santo). it is observed along the coast line of the states of sergipe, alagoas, pernambuco, paraiba and rio grande do n ... | 1992 | 1343914 |
| [bacteria associated with diarrheic episodes in young camels in niger]. | during diarrhoeic episodes of young camels in niger, bacteria such as salmonella enteritidis, e. coli, staphylococcus sp., clostridium perfringens and other bacteria were isolated from stool samples. bacteria roll in this pathology is discussed, and a treatment is proposed, compared with antibiogram results. | 1992 | 1345079 |
| [entomology of onchocercosis in soconusco, chiapas. 6. quantitative studies of the transmission of onchocerca volvulus by 3 species of simuliidae in a community with high endemicity]. | in guatemala, there is no doubt about the participation of simulium ochraceum as vector of onchocerciasis. however, in mexico practically there are not studies focussed to determine the role of this species in the transmission. the objective of the present investigation was to determine which of the 3 species of simulium founded in the soconusco region of chiapas, is the main, and which were secondary in the transmission of onchocerciasis in that area. the locality of morelos, in the huixtla "mu ... | 1992 | 1345118 |
| regulation of anabaena sp. strain pcc 7120 glutamine synthetase activity in a synechocystis sp. strain pcc 6803 derivative strain bearing the anabaena glna gene and a mutated host glna gene. | the glna gene from synechocystis sp. strain pcc 6803 was cloned by hybridization with the glna gene from anabaena sp. strain pcc 7120, and a deletion-insertion mutation of the synechocystis gene was generated in vitro. a strain derived from synechocystis sp. strain pcc 6803 which contained integrated into the chromosome, in addition to its own glna gene, the anabaena glna gene was constructed. from that strain, a synechocystis sp. glna mutant could be obtained by transformation with the inactiva ... | 1992 | 1345914 |
| taxol: a novel radiation sensitizer. | the investigational antineoplastic agent, taxol, a natural product from the yew, taxus sp. l., is currently being evaluated in a series of phase ii clinical trials. to date, the drug has shown activity against ovarian cancer, lung cancer, and melanoma. taxol is a potent microtubule stabilizing agent that selectively blocks cells in the g2 and m phases of the cell cycle and is cytotoxic in a time-concentration dependent manner. it is well known from radiobiological principles that g2 and m are th ... | 1992 | 1346533 |
| correlation of genetic and physical structure in the region surrounding the i2 fusarium oxysporum resistance locus in tomato. | the dominant gene i2 confers on tomato (lycopersicon esculentum) resistance against the fungus fusarium oxysporum f. sp. lycopersici race 2. a restriction fragment length polymorphism (rflp) marker, tg105, has recently been found to be tightly linked to i2. the potential for cloning this gene by a reverse genetics approach prompted us to describe in both genetic and physical detail the region surrounding the i2 locus on chromosome 11. we have analyzed patterns of segregation of rflp markers on c ... | 1992 | 1346546 |
| influence of the size and protonation state of acidic residue 85 on the absorption spectrum and photoreaction of the bacteriorhodopsin chromophore. | the consequences of replacing asp-85 with glutamate in bacteriorhodopsin, as expressed in halobacterium sp. grb, were investigated. similarly to the in vitro mutated and in escherichia coli expressed protein, the chromophore was found to exist as a mixture of blue (absorption maximum 615 nm) and red (532 nm) forms, depending on the ph. however, we found two widely separated pka values (about 5.4 and 10.4 without added salt), arguing for two blue and two red forms in separate equilibria. both blu ... | 1992 | 1346749 |
| isolation and amino acid sequence of a glutamic acid specific endopeptidase from bacillus licheniformis. | an endopeptidase cleaving specifically at the carboxyl side of acidic amino acid residues, preferentially at glutamic acid, has been isolated from a commercial extract obtained by fermentation with bacillus licheniformis. using ion-exchange chromatography and affinity chromatography on bacitracin-sepharose, it was possible, from 100 ml commercial extract, to isolate 100 mg homogeneous enzyme in a yield of 50%. it is the first description of a large-scale isolation of a glu/asp-specific enzyme. t ... | 1992 | 1346764 |
| chromosomal localization of three pulmonary surfactant protein genes in the mouse. | pulmonary surfactant, a protein-phospholipid mixture, maintains surface tension at the lung epithelium/air interface preventing alveolar collapse during respiration. for mammals appropriate developmental production of surfactant is necessary for adaptation to the air breathing environment. deficiency of pulmonary surfactant results in respiratory distress syndrome (rds), a leading cause of death in premature infants. recently, three lung-specific pulmonary surfactant proteins designated sp-a, sp ... | 1992 | 1346779 |
| distribution and vasomotor effects of neuropeptides in angular oculi and facial veins of reindeer. | 1. the vasomotor responses to neuropeptides of the angular oculi and facial veins of reindeer were examined in vitro and correlated with the neuropeptide distribution in the perivascular nerves, as demonstrated by immunohistochemistry. 2. nerves displaying calcitonin gene related peptide (cgrp)- or neuropeptide y (npy)-like immunoreactivity (-li) were observed in the media of both veins, while very few fibers were immunoreactive to vasoactive intestinal polypeptide (vip) or substance p (sp) in e ... | 1992 | 1348461 |
| ultrastructure of tricornia muhezae n. g., n. sp. (microspora, thelohaniidae), a parasite of mansonia africana (diptera: culicidae) from tanzania. | collections of mansonia africana mosquito larvae were made at one site in n.e. tanzania in 1985 and 1987 and from two additional sites, both within about 2 mi of the original one in 1987. an octosporous microsporidian, present at all three sites, was found in both years infecting from 7 to 22% of larvae. spores (stained in giemsa) measured 3.0 microns +/- 0.25 microns x 2.25 microns +/- 0.26 microns. ultrastructurally, spores were seen to have an anterior rim surrounding a depressed area where t ... | 1992 | 1348541 |
| identification of a surface metalloproteinase on 13 species of leishmania isolated from humans, crithidia fasciculata, and herpetomonas samuelpessoai. | promastigotes of thirteen species of leishmania isolated from human patients, as well as l. enriettii, crithidia fasciculata and herpetomonas samuelpessoai, were examined for the expression of an amphiphilic, surface-oriented metalloproteinase by surface radioiodination of living cells, fractionation by triton x-114 extraction and phase separation, and zymogram analysis by fibrinogen-sds-page. in all species of leishmania, and the two monoxenous trypanosomatid parasites of insects, an ectoprotei ... | 1992 | 1348597 |
| exogenous thy-1.1 expression as a marker for thymocyte maturation in transgenic mice. | we established a line of transgenic mice carrying the exogenous mouse thy-1.1 gene (8.2 kb eco ri genomic dna fragment). in these mice, thy-1.1 was expressed on thymocytes but not on peripheral t cells, presumably due to the lack of cis-acting element(s) on the microinjected genomic dna. even in the thymus, however, while most of the cd3/tcr- thymocytes were positive for the transgenic thy-1.1 gene expression, the cd4+ or cd8+ single positive (sp) thymocytes were composed of two groups, one thy- ... | 1992 | 1348881 |
| pertussis toxin-sensitive and pertussis toxin-insensitive inhibition of parietal cell response to glp-1 and histamine. | we have recently shown that in rat parietal cells the glucagon-like peptide 1 (glp-1) variants 7-36 amide, 1-37, and 1-36 amide stimulate h+ production as indirectly measured by [14c]aminopyrine (ap) accumulation. this response to the glp-1 peptides was intracellularly mediated by activation of adenylate cyclase and by adenosine 3',5'-cyclic monophosphate (camp) as second messenger. in the present study, we compared prostaglandin (pg)e2, somatostatin, and the protein kinase a antagonist rp-adeno ... | 1992 | 1348905 |
| epidemiological role of arthropods detectable in health facilities. | a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ... | 1992 | 1350604 |
| identification and analysis of the ret proto-oncogene promoter region in neuroblastoma cell lines and medullary thyroid carcinomas from men2a patients. | the human ret proto-oncogene (proto-ret), encoding a receptor tyrosine kinase, is highly expressed in neuroblastomas, medullary thyroid carcinomas (mtcs) and pheochromocytomas, which are all tumors of cells originating from the neural crest. in studies on the transcription mechanism of proto-ret, we identified the transcription start site and the promoter region by chloramphenicol acetyl transferase (cat) assay. a sequence upstream from the transcription start site (-167 to +98 bp) showed defini ... | 1992 | 1350670 |
| taxol sensitizes human astrocytoma cells to radiation. | taxol is a chemotherapeutic drug which acts by stabilizing microtubules, preventing normal mitosis and resulting in a block of the cell cycle at g2 and m. the drug is isolated from the yew, taxus sp. l., and is currently being evaluated in a series of phase ii and phase iii clinical trials. taxol blocks cells in the most radiosensitive phases of the cell cycle and thus could act as a cell cycle-specific radiosensitizer. we report the results of combined taxol-radiation exposures in the human gra ... | 1992 | 1350755 |
| an immunohistochemical study of the telencephalon of the senegal bichir (polypterus senegalus). | the telencephalon in ray-finned fish (actinopterygians) is everted, in contrast to the evaginated telencephalic hemispheres in all other vertebrates. in the more derived ray-finned fish, the teleosts, proliferation of neurons and their migration from the ependymal zone of the pallium renders comparisons between telencephalic cell groups of the teleosts and members of other vertebrate groups extremely difficult. the telencephalon of polypterus (a primitive living ray-finned fish), although everte ... | 1992 | 1351063 |
| activation of b virus (herpesvirus simiae) in chronically immunosuppressed cynomolgus monkeys. | three of 14 cynomolgus monkeys given the highest dose of an immunosuppressive drug in a 6-month toxicology study developed b virus (herpesvirus simiae) oral lesions after 3 months of dosing. this necessitated early removal of all high-dose monkeys from the study due to concerns related to b virus. the incidence and severity of parasitic (oesphagostomum sp.) lesions of the large intestine were also increased in high-dose animals. both b virus and oesophagostomum are enzootic in macaques, and the ... | 1992 | 1351111 |
| microbiology of postoperative wound infection: a prospective study of 1770 wounds. | a prospective study of postoperative wound infection was carried out over a 12-month period. intra-operative swabs from the patients' anterior nares, the opened viscus and parietes were cultured using standard bacteriological techniques. of the 1770 wounds studied, 167 (9.4%) became infected. wound infection rates, according to clinical wound types, were clean 5.9%, clean-contaminated 10.7%, contaminated 24.3% and dirty 52.9%. the figures according to microbiological wound types were clean 4.7%, ... | 1992 | 1351494 |
| ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-coa hydratases/isomerases, and acyl-coa thioesterases. | we have deduced the nucleotide sequence of the genes encoding the three components of 4-chlorobenzoate (4-cba) dehalogenase from pseudomonas sp. cbs-3 and examined the origin of these proteins by homology analysis. open reading frame 1 (orf1) encodes a 30-kda 4-cba-coenzyme a dehalogenase related to enoyl-coenzyme a hydratases functioning in fatty acid beta-oxidation. orf2 encodes a 57-kda protein which activates 4-cba by acyl adenylation/thioesterification. this 4-cba:coenzyme a ligase shares s ... | 1992 | 1351742 |
| purification and characterization of an agglutinin of the soft coral sinularia species. | a d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral sinularia sp. by affinity chromatography on acid-treated sepharose 4b and by gel filtration on hplc. sinularian was a glycoprotein containing 11% sugar. it gave a single band corresponding to 78 kda in sds-page, irrespective of a treatment with 2-mercaptoethanol. sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human abo erythrocytes. its hemagglutinating activity was ... | 1992 | 1352264 |
| evidence that some frankia sp. strains are able to cross boundaries between alnus and elaeagnus host specificity groups. | phenotypic and genotypic methods were used to prove the existence of frankia strains isolated from an elaeagnus sp. that are able to cross the inoculation barriers and infect alnus spp. also. repeated cycles of inoculation, nodulation, and reisolation were performed under axenic conditions. frankia wild-type strain ufi 13270257 and three of its coisolates did exhibit complete infectivity and effectiveness on elaeagnus spp. and hippophaë rhamnoides and variable infectivity on alnus spp. microscop ... | 1992 | 1352442 |
| a farnesyl arabinoside as an enhancer of glucose transport in rat adipocytes from a soft coral, sinularia sp. | separation of a lipophilic extract of a soft coral, sinularia sp., assayed by enhancement of glucose transport in rat adipocytes, gave farnesyl 4-o-beta-d-arabinopyranosyl-beta-d-arabinopyranoside-2,2',3- triacetate (1a) whose structure was determined by spectroscopy. enhancers of glucose transport may be useful for the prevention and treatment of diabetic disorders. | 1992 | 1353460 |
| isolation and characterization of a ntrc mutant of bradyrhizobium (parasponia) sp. anu289. | a mutant of bradyrhizobium (parasponia) sp. anu289 affected in the regulation of nitrogen metabolism was isolated. the mutant, designated anu293, was unable to induce ammonium transport (amt), nitrate reductase (nr) or glutamine synthetase ii (gsii) activities under conditions that induce these activities in the wild-type. however, glutamine synthetase i (gsi), which is expressed constitutively in the wild-type, was present at normal levels in the mutant. the mutant also retained the ability to ... | 1992 | 1353784 |
| effects of copper, cadmium and nickel on liver and kidney glutathione redox cycle of rats (rattus sp.). | 1. glutathione redox cycle alterations induced by acute treatment of nickel, cadmium and copper, have been investigated in liver and kidney of adult male rats. 2. in liver, nickel treatment decreased gsh and gssg levels, followed by a significant rebound in gsh content. copper produced drastic reduction in the gsh/gssg ratio, while cadmium treatment altered gssg concentrations. 3. in kidney, the glutathione redox cycle remained unaltered after cadmium or nickel exposure, whereas copper caused si ... | 1992 | 1354092 |
| functional maturation of recent thymic emigrants in the periphery: development of alloreactivity correlates with the cyclic expression of cd45rc isoforms. | the transition from fully developed cd4+cd8- single-positive (sp) thymocytes into fully mature recirculating peripheral t cells is both poorly understood with regard to the expression of restricted isoforms (cd45r) of the leukocyte common antigen and in terms of t cell function. the present investigation monitored the extrathymic development of cd4+cd8- sp thymocytes in euthymic recipients using allotype-marked donor cells and monoclonal antibody ox22 which recognizes an epitope on the c exon of ... | 1992 | 1355430 |
| effects of selective tachykinin receptor antagonists on capsaicin- and tachykinin-induced bronchospasm in anaesthetized guinea-pigs. | bronchospasm induced by i.v. injection of equieffective doses of acetylcholine, capsaicin or selective tachykinin receptor agonists ([sar9]sp sulfone or [beta-ala8]neurokinin a (nka-4-10)) (for nk1 and nk2 receptors, respectively) was studied in anaesthetized guinea-pigs. the nk1 and nk2 receptor antagonists, (+/-)-cp96,345 (3 mumol/kg i.v.) and men 10,376 (3 mumol/kg i.v.), selectively abolished the bronchoconstriction induced by the respective agonist, showing that both nk1 and nk2 receptors m ... | 1992 | 1355435 |
| alcohol-preferring rats have fewer dopamine d2 receptors in the limbic system. | dopamine d2 receptors in nucleus accumbens, olfactory tubercle and caudate nucleus of sardinian ethanol-preferring (sp), and non-preferring (snp) rats were compared by using [3h]ym-09151-2 binding. sp rats exhibited, in each area, lower density of d2 receptors than snp and unselected wistar (uw) rats. the results suggest that reduction in d2 receptors in sp rats may be relevant to their innate preference for alcohol. | 1992 | 1355967 |
| plasmid mediated metal and antibiotic resistance in marine pseudomonas. | pseudomonas sp isolated from the bay of bengal (madras coast) contained a single large plasmid (pmr1) of 146 kb. plasmid curing was not successful with mitomycin c, sodium dodecyl sulfate, acridine orange, nalidixic acid or heat. transfer of mercury resistance from marine pseudomonas to escherichia coli occurred during mixed culture incubation in liquid broth at 10(-4) to 10(-5) ml(-1). however, transconjugants lacked the plasmid pmr1 and lost their ability to resist mercury. transformation of p ... | 1992 | 1356041 |
| hypotensive effect associated with a phospholipase c-delta 1 gene mutation in the spontaneously hypertensive rat. | to identify the genes responsible for blood pressure in the spontaneously hypertensive rat strain, we performed a cosegregation analysis between the genotype and blood pressure in a set of male f2 rats obtained by crossmating shr with wistar-kyoto rats, a parental normotensive strain. our investigation revealed that the phospholipase c-delta 1 polymorphism, which resulted in missense mutation, cosegregates with the lower blood pressure in shr, and that plc-delta 1 gene is located on chromosome 8 ... | 1992 | 1358065 |
| nucleotide sequence and expression in escherichia coli of the cephalosporin acylase gene of a pseudomonas strain. | the gene encoding cephalosporin acylase, which hydrolyzes 7-beta-(4-carboxybutanamido)-cephalosporanic acid (gl-7aca) to 7-aminocephalosporanic acid (7aca) and glutaric acid, was cloned from a pseudomonas sp. strain v22 and expressed in escherichia coli, in a two-cistron system, and the enzyme was purified and characterized. the purified enzyme was composed of two non-identical subunits, their molecular weights were estimated by sds-page to be 40,000 and 22,000, and had a pi of 4.6. the amino ac ... | 1992 | 1358202 |
| transient expression of somatostatin peptide is a widespread feature of developing sensory and sympathetic neurons in the embryonic rat. | previous studies from this and other laboratories demonstrated that many embryonic sensory ganglion cells in the rat transiently express the catecholamine synthesizing enzyme tyrosine hydroxylase (th), a trait not expressed by most mature sensory neurons. we, therefore, sought to determine whether transient expression was uniquely associated with catecholaminergic traits, or, alternatively, whether embryonic ganglion cells transiently expressed peptidergic properties as well. of the four peptide ... | 1992 | 1359005 |
| effects of exercise and food restriction in pregnant and newborn rats. pre-pregnancy maximum oxygen consumption. | 1. in the present study, the effects of exercise and food restriction in pregnant and newborn rats were investigated. 2. the following groups were formed: adequate food supply, with and without exercise (ae and an) and 30% food restriction, with and without exercise (re and rn). 3. exercise was performed throughout the pregnancy on a treadmill at a speed of 18 m/min for 30 min/day, which represented 84% of maximum vo2. 4. the results show that food restriction affected body weight gain while exe ... | 1992 | 1359947 |
| inhibitory effect of a microcystis sp (cyanobacteria) toxin on development of preimplantation mouse embryos. | 1. a soluble toxin, purified from the algae bloom of an eutrophic lake dominated by microcystis, is a very effective inhibitor of early embryo development in a dose-response relationship. 2. two- and 8-cell mouse embryos under the influence of microcystis toxin do not reach the developmental stages of morula and blastocyst, respectively. 3. actin cortex is disorganized without change in the microtubules structure. 4. results are discussed in terms of the possible mechanisms by which the toxin ar ... | 1992 | 1360361 |
| patterns of glutamate, glycine, and gaba immunolabeling in four synaptic terminal classes in the lateral superior olive of the guinea pig. | the goal of this study was to correlate synaptic ultrastructure with transmitter specificity and function in the lateral superior olive (lso), a nucleus that is thought to play a major role in sound localization. this was accomplished by means of postembedding immunogold immunocytochemistry. four classes of synaptic terminals were identified in the lso. they were distinguishable from one another both morphologically and on the basis of their different patterns of immunolabeling for glutamate, gl ... | 1992 | 1360986 |
| characterization of the murine icam-1 gene. | intercellular adhesion molecule-1 (icam-1, cd54) is a cell adhesion molecule that interacts with the leukocyte beta 2 integrins, lfa-1 and mac-1. murine inflammatory models are being utilized increasingly to define the role that icam-1 induction plays in the initiation of inflammation. we have isolated murine genomic clones that contain the icam-1 gene including over 2 kb of 5' flanking sequence. the gene for murine icam-1 spans over 13 kb and is composed of seven exons and six introns. each of ... | 1992 | 1362180 |
| molecular characterization of the gene encoding glutamine synthetase in the cyanobacterium calothrix sp. pcc 7601. | in order to study the regulation of the synthesis of glutamine synthetase in response to changes in environmental parameters (light and nitrogen sources), we have cloned and sequenced the glna gene from the filamentous cyanobacterium calothrix pcc 7601. this gene consists of 472 codons and encodes a polypeptide of m(r) 52,290 highly homologous to that from anabaena pcc 7120, but more distant from those identified from other procaryotes. the relative abundance of the two glna transcripts (1.6 and ... | 1992 | 1362348 |
| colocalization of peptide- and tyrosine hydroxylase-like immunoreactivities with fos-immunoreactive neurons in rat central amygdaloid nucleus after immobilization stress. | the central amygdaloid nucleus (ace) is part of the amygdaloid body, and it has been shown to participate in several stress related reactions. the ace is densely innervated by tyrosine hydroxylase- (th), corticotropin releasing factor- (crf), calcitonin gene-related peptide- (cgrp), neurotensin- (nt), somatostatin- (som), enkephalin- (enk), substance p- (sp), vasoactive intestinal polypeptide- (vip) and cholecystokinin- (cck) immunoreactive (ir) nerve terminals. in addition, the ace contains num ... | 1992 | 1362516 |
| the management of acute, serous and chronic otitis media: the role of anaerobic bacteria. | otitis media (om) is a common childhood disease and one which can cause significant morbidity. a knowledge of the pathogens responsible for om enables the most appropriate treatment regimen to be selected and thus minimizes further complications which may require hospital admission and surgery. the microbiology of acute, serous and chronic om is reviewed, with particular regard to the role of anaerobic bacteria. anaerobes, mainly gram-positive cocci, have been recovered from 25% of the ear aspir ... | 1992 | 1362753 |
| water mite (arrenurus sp.) parasitizing mosquitoes in district shahjahanpur, u.p. | | 1992 | 1363318 |
| mycotic aneurysm rupture: report of four cases. | "mycotic" aneurysm was originally described by osler in 1885. it occurs in a normal or atherosclerotic artery from septic emboli in patients with infective endocarditis. however, now the term "mycotic" aneurysm is applied to all cases of aneurysms caused by any organisms. from september 1988 to november 1990, four cases of ruptured mycotic aneurysm were diagnosed at our institute. three were males and one was a female; they were elderly with atherosclerosis of the aorta. the diagnosis was establ ... | 1992 | 1364221 |
| the oxygen-centered radicals scavenging activity of sulfasalazine and its metabolites. a direct protection of the bowel. | oxygen-centered radicals, such as superoxide (o2-) and hydroxyl radicals (.oh) generated by phagocytes have been suggested to be involved in the pathogenesis of chronic inflammations of the bowel, such as crohn's disease and colitis ulcerosa. recently, sulfasalazine (sasp) and its metabolites have been reported to exert their effects as a direct scavenger of oxygen-centered radicals in the bowel. to scavenge oxygen-centered radicals in vivo, however, sasp and its metabolites have to react with o ... | 1992 | 1364279 |
| [fungi isolated from diseased medicinal plants]. | one hundred and forty-four fungal isolates were obtained from diseased paeonia albiflora pall. var. trichocarpa bung., astragalus membranaceus bung., lithospermum erythrorhizon sieb. et zucc., ledebouriella seseloides wolff and bupleurum falcatum l. which were collected in the test field of tsukuba medicinal plant research station, national institute of hygienic sciences. most of them were identified into 15 genera containing 8 species. fungal species presumed to be pathogens of the host plants ... | 1992 | 1364438 |
| localization of methanol dehydrogenase in two strains of methylotrophic bacteria detected by immunogold labeling. | antibodies to methanol dehydrogenase purified from methylobacterium sp. strain am1 and methylomonas sp. strain a4 were raised. the antibody preparations were used in indirect immunogold labeling studies. with this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of the methylotroph methylobacterium sp. strain am1 and to the intracytoplasmic membrane of the methanotroph methylomonas sp. strain a4. antibody cross-reactivity to other methylotrophic ... | 1992 | 1365400 |
| trimethyl lead degradation by free and immobilized cells of an arthrobacter sp. and by the wood decay fungus phaeolus schweinitzii. | the continuing production of leaded petrol generates liquid wastes containing recalcitrant trialkyl lead, for which no suitable chemical treatment has been formulated. this investigation explores the feasibility of using microorganisms to catalyse the rate-limiting step of trimethyl lead degradation to dialkyl lead; this disproportionates chemically to give, ultimately, pb2+ which is treatable by classical methods. an arthrobacter sp. and a wood decay macrofungus, phaeolus schweinitzii provide n ... | 1990 | 1366364 |
| isolation and characterization of a 2-(2,4-dichlorophenoxy) propionic acid-degrading soil bacterium. | the 2-(2,4-dichlorphenoxy)propionic acid (2,4-dp)-degrading bacterial strain mh was isolated after numerous subcultivations of a mixed culture obtained by soil-column enrichment and finally identified as flavobacterium sp. growth of this strain was supported by 2,4-dp (maximum specific growth rate 0.2 h-1) as well as by 2,4-dichlorophenoxyacetic acid (2,4-d), 4-(2,4-dichlorophenoxy)butyric acid (2,4-db), and 2-(4-chloro-2-methylphenoxy)propionic acid (mcpp) as sole sources of carbon and energy u ... | 1990 | 1366457 |
| kinetic studies of acetate fermentation by methanosarcina sp. msta-1. | the effect of three parameters (initial acetate concentration, temperature and ph) on the acetoclastic reaction was studied with the thermophilic methanogenic bacterium methanosarcina sp. msta-1. the optimum temperature for growth ranged around 55 degrees c, and optimum ph was 6.5-7.5, giving a minimum generation time of 12.6-13.9 h (mu max = 0.050-0.055 h-1) and a maximum value of the specific acetate consumption rate (qmaxs) of 14-20 mmol/g cells per hour. contrary to the methane yield, the gr ... | 1990 | 1366458 |
| bioconversions in aqueous two-phase systems. | bioconversions involving enzymes and/or microbial cells in aqueous two-phase systems are reviewed. the partitioning of biocatalysts, substrates, and products is discussed in relation to their size. the efficiency of retaining biocatalysts in aqueous two-phase systems is summarized in relation to other methods of recirculating. the influence of phase components on the activity and the stability of enzymatic biocatalysts is exemplified with penicillin acylase and the cellulolytic enzyme system, an ... | 1990 | 1366522 |
| expression of an erwinia sp. gene encoding diphenyl ether cleavage in escherichia coli and an isolated acinetobacter strain pe7. | an acinetobacter strain pe7 with the ability to grow on salicyclic acid and to degrade diphenyl ethers was isolated from a petroleum waste pit in louisiana. a cloned erwinia sp. dpe gene encoding diphenyl ether cleavage was introduced into pe7 in order to enhance its degradative ability. a broad-host-range expression plasmid, pdpe2388, was constructed by inserting an sspi-hpai fragment from a dpe gene-containing plasmid, pdpe7321, into the kanamycin resistance gene of plasmid pkt230. the dna fra ... | 1990 | 1366541 |
| nadh production from nad+ with a formate dehydrogenase system involving immobilized cells of a methylotrophic arthrobacter strain. | a convenient and economical method of nadh production from nad+ has been established using a formate dehydrogenase system involving immobilized cells of a methanol-utilizing bacterium. arthrobacter sp, km62. four kinds of cell entrapment were studied. an immobilized cell preparation showing a high nadh production activity was obtained by entrapment in a kappa-carrageenan gel lattice. the nadh-producing activity of the immobilized cells was investigated under various conditions. the nadh-producin ... | 1990 | 1366576 |
| induction and purification of kievitone hydratase from fusarium solani f. sp. phaseoli. | fusarium solani f. sp. phaseoli is capable of detoxifying the major isoflavonoid phytoalexins produced by its host plant phaseolus vulgaris. one of the enzymic activities involved is kievitone hydratase (khase), a secreted glycoprotein which catalyses the conversion of kievitone to the less fungitoxic derivative, kievitone hydrate. even under conditions of substrate induction, the enzyme is expressed at levels that are too low for satisfactory purification. therefore, several other isoflavonoids ... | 1990 | 1366757 |
| cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins. | a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ... | 1990 | 1366910 |
| production of l-tryptophan from d,l-5-indolylmethylhydantoin by resting cells of a mutant of arthrobacter species (dsm 3747). | the reaction parameters and the stereospecificity of the enzymatic cleavage of d,l-5-indolylmethylhydantoin in producing l-tryptophan with resting cells of arthrobacter sp. dsm 3747 were studied. when intact cells were tested, the optimal ph was between 8.5 and 9.0 and the optimal temperature was 50 degrees c. both, l-n-carbamoylase and hydantoinase could be stabilized over 24 h at 30 and 40 degrees c by the addition of d,l-5-indolylmethylhydantoin. furthermore, the hydantoinase was stable over ... | 1990 | 1366911 |
| cp-82,996, a novel diglycoside polyether antibiotic related to monensin and produced by actinomadura sp. | a new polyether antibiotic cp-82,996 (c50h86o16) was isolated by solvent extraction from the fermentation broth of actinomadura sp. (atcc 53764). following purification by silica gel column chromatography and crystallization, the structure of cp-82,996 was determined by a single crystal x-ray analysis. the structure is closely related to monensin, but is unique in that it contains two sugar groups, whereas monensin has none. the 1h and 13c nmr chemical shifts and assignments for cp-82,996 were e ... | 1990 | 1366920 |
| coupled reductive and oxidative degradation of 4-chloro-2-nitrophenol by a co-immobilized mixed culture system. | the restriction of oxygen transfer in ca-alginate beads used for the immobilization of microbial cells was applied to a coupled reductive and oxidative microbial degradation of the xenobiotic 4-chloro-2-nitrophenol (cnp). the conversion of cnp by enterobacter cloacae under anaerobic conditions led to the formation of 4-chloro-2-aminophenol (cap, 81%) and 4-chloro-2-acetaminophenol (caap, 16%) after 50 h incubation. cap, the main reduction product, was further degraded under aerobic conditions by ... | 1990 | 1366971 |
| dna sequence analysis of endoglucanase genes from pseudomonas fluorescens subsp. cellulosa and pseudomonas sp. ncib 8634. | the dna of two previously isolated recombinant clones, one from pseudomonas sp. ncib 8634 (= cellvibrio mixtus) (ppc71) and another from pseudomonas fluorescens subsp. cellulosa (ppfc4) that express endoglucanase activity in e. coli was sequenced. plasmid ppc71 had three open reading frames, two of which include portions of plasmid pbr322. the third open reading frame occurs entirely within the pseudomonas dna insert and encodes a protein with a molecular mass of 5845 da. the dna insert in ppfc4 ... | 1990 | 1366996 |
| degradation of isomeric monochlorobenzoates and 2,4-dichlorophenoxyacetic acid by a constructed pseudomonas sp. | a 4-chlorobenzoate-degrading pseudomonas sp. us1 was mated with a strain of escherichia coli jmp 397 (harbouring the plasmid pjp4). an ex-conjugant designated pseudomonas sp. us1 ex that could utilize all the isomeric monochlorobenzoates and 2,4-dichlorophenoxyacetate was obtained. the ex-conjugant released stoichiometric amounts of chloride when grown on these chloroaromatics as sole sources of carbon and energy. | 1991 | 1367232 |
| wolffish antifreeze protein from transgenic drosophila. | we have expressed two antifreeze protein genes from the atlantic wolffish, anarhichas lupus, in drosophila melanogaster by placing them under the divergent transcriptional control of the host yolk polypeptide (1 and 2) gene promoters. both genes were joined to the central promoter region by fusion within the dna encoding the signal polypeptides. the chimeric genes were introduced into flightless mutant drosophila through p-element transformation. transformed adult females from individual lines a ... | 1990 | 1367435 |
| isolation and characterization of acetonitrile utilizing bacteria. | bacteria utilizing high concentrations of acetonitrile as the sole carbon source were isolated and identified as chromobacterium sp. and pseudomonas aeruginosa. maximum growth was attained after 96 h of incubation and p. aeruginosa grew slightly faster than chromobacterium sp. the strains were able to grow and oxidize acetonitrile at concentrations as high as 600 mm. however, higher concentrations inhibited growth and oxygen uptake. degradation studies with (14c)acetonitrile indicated 57% of ace ... | 1990 | 1367463 |
| detection of listeria species and listeria monocytogenes using polymerase chain reaction. | five oligonucleotide sequences are described that were used as primers in the polymerase chain reaction (pcr) to amplify specific sequences from listeria dna. when all five primers were used in combination, three pcr products were possible; a listeria specific product that occurs with dna from any listeria sp., a listeria monocytogenes specific product that occurs only in the presence of dna from this organism and a universal product that is found using dna from any bacterial source. the occurre ... | 1990 | 1367467 |
| improvement of glidobactin a production by polyangium brachysporum. | glidobactins a, b, and c are lipopeptide antitumor antibiotics produced by the gliding bacterium polyangium brachysporum sp. nov. no. k481-b101. the production of glidobactin a was examined in shake flasks and laboratory fermentors. medium screening and optimization led to approximately five fold increases in glidobactin a titers in shake flasks and a ten fold increase in titers in 40-l batch fermentations. utilization of a stepped glucose feeding protocol resulted in glidobactin a titers of 186 ... | 1990 | 1367482 |
| enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein. | using molecular genetic techniques, a fusion protein has been produced which contains the cellulose-binding domain (cbd) of an exoglucanase (cex) from cellulomonas fimi fused to a beta-glucosidase (abg) from agrobacterium sp. the cbd functions as an affinity tag for the simultaneous purification and immobilization of the enzyme on cellulose. binding to cellulose was stable for prolonged periods at temperatures from 4 degrees c to at least 50 degrees c, at ionic strengths from 10 mm to greater th ... | 1991 | 1367528 |
| biodegradation of 4-chlorophenol by adsorptive immobilized alcaligenes sp. a 7-2 in soil. | alcaligenes sp. a 7-2 immobilized on granular clay has been applied in a percolator to degrade 4-chlorophenol in sandy soil. good adsorption rates on granular clay were achieved using cell suspensions with high titres and media at ph 8.0. the influence of various parameters such as aeration rate, ph, temperature, concentration of 4-chlorophenol and size of inoculum on the degradation rate were investigated. during fed-batch fermentations under optimal culture conditions, concentrations of 4-chlo ... | 1991 | 1367580 |
| on-line monitoring of marine cyanobacterial cultivation based on phycocyanin fluorescence. | a novel on-line fluorescence monitoring system for marine cyanobacterial cultivation was developed. this method is based on the measurement of intracellular phycocyanin content, which is the major light harvesting protein. a fluorescence spectrophotometer, equipped with a flow cell connected with a culture liquid recycling tube was used. experiments were carried out using a marine unicellular cyanobacteria synechococcus sp. nkbg 042902 isolated from japanese coastal sea water. we have optimized ... | 1991 | 1367694 |
| production of androsta-1,4-diene-3,17-dione from cholesterol using immobilized growing cells of mycobacterium sp. nrrl b-3683 adsorbed on solid carriers. | living cells of mycobacterium sp. nrrl b-3683 were immobilized by adsorption on different types of solid carriers in order to produce androsta-1,4-diene-3,17-dione (add) from cholesterol. activated alumina proved to be the most preferred carrier for long-term operation when glucose and peptone were added to the reaction medium. in a repeated-batch process, the maximum productivity of add was about 0.19 g/l per day with a molar conversion rate of 77% when 1.0 g/l of cholesterol was added to the r ... | 1992 | 1368063 |
| n-terminal amino acid sequence of brevibacterium sp. r312 wide-spectrum amidase. | a wide-spectrum amidase from brevibacterium sp. r312 was partially purified. the enzyme subunit was purified by reversed phase hplc and the n-terminal amino acid sequence was found to be identical to that of pseudomonas aeruginosa aliphatic amidase. | 1991 | 1368108 |
| degradation of 3-chloro-2-methylpropionic acid by xanthobacter sp. cimw 99. | both stereoisomers of 3-chloro-2-methylpropionic acid (cmpa) and its methyl esters (mecmpa) serve as growth substrates for a bacterial isolate (xanthobacter sp. cimw 99) when supplied as sole source of carbon and energy. biodegradation of dl-cmpa and dl-mecmpa was shown to be via a common pathway; an initial, constitutive, esterase converted the methyl ester to the corresponding carboxylic acid. further metabolism required the activation of cmpa involving a coa-, atp-, mg(2+)-dependent chloroacy ... | 1991 | 1368112 |
| ph-dependent flagella formation by facultative alkaliphilic bacillus sp. c-125. | a facultative alkaliphilic strain of bacillus sp. c-125 grown at alkaline ph had many sinuous peritrichous flagella and was highly motile. however, most of the cells grown initially at ph 7 were non-motile and possessed few straight flagella. the amount of flagellin was low when the organism was grown at ph 7, suggesting that non-motility is due to poor synthesis of flagellin. the molecular mass of the flagellin was 37 kda and the isoelectric point was ph 5.0. the amino acid composition of the f ... | 1992 | 1368134 |
| cloning and nucleotide sequence of the maltopentaose-forming amylase gene from pseudomonas sp. ko-8940. | the gene coding for the maltopentaose-(g5)-forming amylase of pseudomonas sp. ko-8940 was cloned into escherichia coli and its nucleotides were sequenced. it was expected that a long open reading frame composed of 1,842-bp that encoded 614 amino acid residues for secretory precursor polypeptide including the typical signal sequence with an nh2-terminal was the gene. an extract of escherichia coli carrying the cloned g5-forming amylase gene had amylolytic activity with which produced only g5 from ... | 1992 | 1368136 |
| initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acids, and orthanilic acid in alcaligenes sp. strain o-1. | alcaligenes sp. strain o-1 grew with benzene sulfonate (bs) as sole carbon source for growth with either nh4+ or nh4+ plus orthanilate (2-aminobenzene sulfonate, os) as the source(s) of nitrogen. the intracellular desulfonative enzyme did not degrade 3- or 4-aminobenzene sulfonates in the medium, although the enzyme in cell extracts degraded these compounds. we deduce the presence of a selective permeability barrier to sulfonates and conclude that the first step in sulfonate metabolism is transp ... | 1990 | 1368142 |
| ether-cleaving enzyme and diol dehydratase involved in anaerobic polyethylene glycol degradation by a new acetobacterium sp. | a strictly anaerobic, homoacetogenic bacterium was enriched and isolated from anoxic sewage sludge with polyethylene glycol (peg) 1000 as sole source of carbon and energy, and was assigned to the genus acetobacterium on the basis of morphological and physiological properties. the new isolate fermented ethylene glycol and peg's with molecular masses of 106 to 1000 to acetate and small amounts of ethanol. the peg-degrading activity was not destroyed by proteinase k treatment of whole cells. in cel ... | 1991 | 1368155 |
| nucleotide sequence of the gene for an alkaline endoglucanase from an alkalophilic bacillus and its expression in escherichia coli and bacillus subtilis. | the gene for an alkaline endoglucanase from the alkalophilic bacillus sp. ksm-64 was cloned into the hindiii site of pbr322 and expressed in escherichia coli hb101. the nucleotide sequence of a 4.1-kb region of the hindiii insert had two open reading frames, orf-1 and orf-2. the protein deduced from orf-1 was composed of 244 amino acids with an m(r) of 27,865. subcloning analysis proved that the alkaline endoglucanase was encoded by orf-2 (822 amino acids with an m(r) of 91,040). upstream from o ... | 1992 | 1368251 |
| purification and characterization of a thermostable alkaline protease from alkalophilic thermoactinomyces sp. hs682. | protease secreted into the culture medium by alkalophilic thermoactinomyces sp. hs682 was purified to an electrophoretically homogeneous state through only two chromatographies using butyl-toyopearl 650m and sp-toyopearl 650s columns. the purified enzyme has an apparent relative molecular mass of 25,000 according to gel filtration on a sephadex g-75 column and sds-page and an isoelectric point above 11.0. its proteolytic activity was inhibited by active-site inhibitors of serine protease, dfp an ... | 1992 | 1368301 |
| substrate specificity of thermostable alkaline protease from bacillus sp. no. ah-101. | | 1992 | 1368308 |
| molecular cloning and expression of a streptomyces sarcosine oxidase gene in streptomyces lividans. | a genomic library of streptomyces sp. kb210-8sy, prepared in the plasmid vector pacyc184, was screened to obtain the gene encoding sarcosine oxidase with probes based on the amino acid sequence of the protein. a plasmid psoxs13, which was isolated from a clone identified by hybridization with the probes, contained a 8.4-kb insert of streptomyces dna. when the 2.0-kb miui/ecorv dna fragment of psoxs13 was inserted into the streptomyces vector pij680 and introduced into s. lividans, the transforma ... | 1992 | 1368326 |
| action pattern of bacillus sp. no. 7-m chitosanase on partially n-acetylated chitosan. | the hydrolyzate of partially n-acetylated chitosan by bacillus sp. no. 7-m chitosanase was separated by gel filtration on bio-gel p-2. sugar compositions and sequences of the oligosaccharides were identified by exo-splitting with beta-glcnase, fast atom bombardment mass spectroscopy, and proton nmr spectroscopy. in addition to chitooligosaccharides, (glcn)2, (glcn)3, and (glcn)4, hetero-chitooligosaccharides such as (glcn)2.glcnac.(glcn)2, glcn.glcnac.(glcn)3, (glcn)2.glcnac.(glcn)3, and glcn.gl ... | 1992 | 1368330 |
| cloning, expression, and characterization of a minor alkaline protease from bacillus sp. no. ah-101. | | 1992 | 1368333 |
| oxygen-dependent desulphation of monomethyl sulphate by agrobacterium sp. m3c. | agrobacterium sp. m3c, previously isolated from canal-water for its ability to grow on monomethyl sulphate, degraded this ester with stoichiometric liberation of inorganic sulphate. in contrast with the biodegradation of monomethyl sulphate in hyphomicrobium sp., and of other longer-chain alkyl sulphates in pseudomonas spp., the pathway in agrobacterium appeared not to involve a sulphatase enzyme capable of catalysing ester-bond hydrolysis. no such sulphatase was detectable under a range of cond ... | 1990 | 1368469 |
| identification of metabolites from degradation of naphthalene by a mycobacterium sp. | a mycobacterium sp. isolated from oil-contaminated sediments was previously shown to mineralize 55% of the added naphthalene to carbon dioxide after 7 days of incubation. in this paper, we report the initial steps of the degradation of naphthalene by a mycobacterium sp. as determined by isolation of metabolites and incorporation of oxygen from 18o2 into the metabolites. the results indicate that naphthalene is initially converted to cis- and trans-1,2-dihydroxy-1,2-dihydronaphthalene by dioxygen ... | 1990 | 1368473 |
| evidence for two distinct phosphonate-degrading enzymes (c-p lyases) in arthrobacter sp. glp-1. | arthrobacter sp. glp-1 can utilize a wide range of organophosphonates as its sole source of phosphorus. the in-situ formation of sarcosine and methane from glyphosate and methanephosphonic acid respectively was studied. these two processes are differentially induced during phosphorus-deprivation. methanephosphonic acid strongly inhibits glyphosate degradation (i50 10 microm), but glyphosate has very little effect on methane generation (i50 150 mm). the pattern of inhibition by other organophosph ... | 1991 | 1368477 |
| beta-eliminative cleavage of the acidic polysaccharide of fusarium sp. m7-1 by an enzyme preparation of cellulomonas sp. | by digestion with an enzyme preparation derived from a culture filtrate of cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and beta 1----2 mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of fusarium sp. m7-1 by acetolysis. the unsaturated disaccharide produced was isolated and identified as 4-deoxy-l-threo-hex-4-enopyranouronosyl alpha(1----2)-d-galactose. the same unsaturated sugar linked to the polysa ... | 1990 | 1368507 |
| structure of rice-straw arabinoglucuronoxylan and specificity of streptomyces xylanase toward the xylan. | rice-straw treated with 5% ammonia water was hydrolyzed with the beta-xylanase of streptomyces sp. e-86, and four kinds of hetero-oligosaccharides, two arabinoxylo- and two glucuronoxylo-oligosaccharides, were isolated from the hydrolysate by charcoal column, gel filtration, ion-exchange, and aminopropyl silica column chromatographies. the structures of the oligosaccharides were identified as 3(2)-alpha-l-arabinofuranosyl-xylobiose, 3(2)-alpha-l-arabinofuranosylxylotriose, 2(3)-4-o-methyl-alpha- ... | 1990 | 1368510 |
| comparison of cd composition produced by chimeric cgtases. | using 12 chimeric cyclomaltodextrin glucanotransferases (cgtases) constructed from two genes, the cgtase gene from the alkalophilic bacillus sp. strain no. 38-2 (cgtase 38-2 gene) and the cgtase gene from the alkalophilic bacillus sp. strain no. 17-1 (cgtase 17-1 gene), we compared the effect of those chimeric enzymes on cyclodextrin (cd) production, especially on the composition of the cds produced. it was found that the n-terminal and the c-terminal segments were important for cd production. c ... | 1990 | 1368519 |
| expression of the beta-cyclodextrin glucanotransferase gene of an alkalophilic bacillus sp. #1011 in escherichia coli cells and characterization of the synthesized enzyme. | to express efficiently the gene for extracellular beta-cyclodextrin glucanotransferase (beta-cgtase) of an alkalophilic bacillus sp. #1011 using the e. coli promoters (tac, trp and pl promoters), three dna fragments starting from the nucleotide positions +1, -18, and -48 of the translation initiation site of the gene were prepared and they were fused with the promoters. the maximum production of the enzyme, which was located mainly (90%) in the periplasm of the e. coli strain, was observed in th ... | 1990 | 1368532 |
| purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1. | arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ... | 1990 | 1368550 |
| purification and some properties of endo-1,3-beta-d-xylanase from pseudomonas sp. pt-5. | an endo-1,3-beta-d-xylanase (1,3-beta-d-xylan xylanohydrolase, ec 3.2.1.32) was purified from the culture fluid of pseudomonas sp. pt-5 by ammonium sulfate fractionation, deae-sepharose cl-6b, toyopearl hw-50s, and butyl-toyopearl 650 m column chromatography. the purified enzyme gave a single band on polyacrylamide gel disc electrophoresis and its molecular weight was 35,000 by sds-polyacrylamide gel electrophoresis. the enzyme was stable from ph 5.5 to 8.0 and had its maximum activity at ph 7.5 ... | 1990 | 1368551 |
| cloning and expression of the creatinase gene from flavobacterium sp. u-188 in escherichia coli. | the gene coding for creatinase (creatine amidinohydrolase, ec 3.5.3.3) was isolated from flavobacterium sp. u-188. the primary structure of creatinase deduced from the nucleotide sequence showed a protein (molecular weight, 42, 651) composed of 378 amino acids. the creatinase gene was over-expressed in escherichia coli under the control of the lac promoter and the amount of this enzyme was over 20% of the soluble protein in the cell. | 1990 | 1368564 |
| identification of o-alpha-d-glucopyranosyluronate-(1----2)-d-galactose isolated from the acidic polysaccharide of fusarium sp. m7-1. | | 1990 | 1368570 |