| isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (bubalus bubalis) bull spermatozoa. | this study was designed to investigate the occurrence of bacterial species in water buffalo semen at the time of collection/processing and to assess the efficacy of some selected antibiotics (gtls; gentamycin, tylosin and linco-spectin or sp; streptomycin and penicillin) in cryodiluent on bacterial control and quality including in vivo fertility of buffalo spermatozoa. for this purpose, four experiments were conducted. in experiment 1, a total of 11 bacterial species were isolated from buffalo e ... | 2016 | 26892806 |
| evidence of fasciola spp. resistance to albendazole, triclabendazole and bromofenofos in water buffaloes (bubalus bubalis). | fasciolosis caused by fasciola spp. is considered the most important helminth infection of ruminants in tropical countries. anthelmintic resistance has become a global concern. this study compared the efficacy of the commonly used anthelmintics, determined the toxicity level and any indication of resistance. thirty two water buffaloes naturally-infected with fasciola spp. were used to determine the efficacy of triclabendazole (tbz), albendazole (abz), and bromofenofos (bro) using fecal egg count ... | 2015 | 26878627 |
| caspase-9 inhibitor z-lehd-fmk enhances the yield of in vitro produced buffalo (bubalus bubalis) pre-implantation embryos and alters cellular stress response. | the present investigation was done to study the effect of caspase-9 inhibitor z-lehd-fmk, on in vitro produced buffalo embryos. z-lehd-fmk is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival. buffalo ovaries were collected from slaughterhouse and the oocytes were subjected to in vitro maturation (ivm), in vitro fertilization (ivf) and in vitro culture (ivc). the culture medium was supplemented with z-lehd-fmk at different concentrations i ... | 2016 | 26850530 |
| comparative proteomic analysis of mature and immature oocytes of the swamp buffalo (bubalus bubalis). | maternal protein components change markedly during mammalian oogenesis. many of these proteins have yet to be characterized and verified. in this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the swamp buffalo (bubalus bubalis). proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. image analysis indicated that 17 proteins were differentially express ... | 2016 | 26784167 |
| heat stress and antioxidant enzyme activity in bubaline (bubalus bubalis) oocytes during in vitro maturation. | in vitro environments like heat stress usually increase the production of reactive oxygen species in bubaline oocytes which have been implicated as one of the major causes for reduced developmental competence. oocytes during meiotic maturation are sensitive to oxidative stress, and heat stress accelerates cellular metabolism, resulting in the higher production of free radicals. therefore, the aim of present work was to assess the impact of heat stress during meiotic maturation on bubaline cumulu ... | 2016 | 26781547 |
| evaluation of analgesic effect of caudal epidural tramadol, tramadol-lidocaine, and lidocaine in water buffalo calves (bubalus bubalis). | aim of this study was to compare the analgesic effect of tramadol and a combination of tramadol-lidocaine with that produced by lidocaine administration in the epidural space in buffalo calves. in a prospective randomized crossover study, ten male buffalo calves were used to compare the epidural analgesic effect of tramadol (1 mg/kg) and tramadol-lidocaine combination (0.5 mg/kg and 0.11 mg/kg, resp.) with that produced by 2% lidocaine (0.22 mg/kg). loss of sensation was examined by pin-prick te ... | 2015 | 26770870 |
| l-cysteine improves antioxidant enzyme activity, post-thaw quality and fertility of nili-ravi buffalo (bubalus bubalis) bull spermatozoa. | the effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, post-thaw quality parameters and in vivo fertility of nili-ravi buffalo bull spermatozoa were studied. semen samples from 4 buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of l-cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 mm) and frozen in 0.5-ml french straws. the antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase ... | 2016 | 26762772 |
| first report of cryptosporidium species in farmed and wild buffalo from the northern territory, australia. | a molecular epidemiological survey of cryptosporidium from water buffalo (bubalus bubalis) in the northern territory in australia was conducted. fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18s quantitative pcr (qpcr). positives were typed by sequence analysis of 18s nested pcr products. the qpcr prevalence of cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. sequence analysis identified two species: c. pa ... | 2016 | 26758449 |
| biochemical, immunological and kinetic characterization and partial sequence analysis of a thiol proteinase inhibitor from bubalus bubalis kidney: an attempt targeting kidney disorders. | in the present study a thiol proteinase inhibitor was isolated from buffalo kidney making use of ammonium sulphate precipitation and gel filtration chromatography on sephacryl s-100hr column. purified inhibitor is homogeneous as it displayed a single band in gel electrophoresis both under reducing and non-reducing environment and is of 65kda as revealed by gel filtration and sds page. kinetic studies revealed the presence of reversible accompanied with competitive mode of inhibition; showing max ... | 2017 | 26751400 |
| 209 production of buffalo (bubalus bubalis) embryos containing human lysozyme gene. | lysozyme is a ubiquitous enzyme found in all major taxa of living organisms with a diverse role in human and animal health. it plays an important role as a nonspecific immune factor and anti-inflammatory factor and is a part of the innate immune system. research on the expression of recombinant human lysozyme is, thus, potentially valuable to the dairy industry. therefore, the present study was carried out to observe the developmental competence and quality of cloned embryos containing the human ... | 2016 | 28279006 |
| 198 successful transplantation of buffalo (bubalus bubalis) germ cells to homologous recipients. | transplantation of isolated germ cells from a fertile donor male into the seminiferous tubules of infertile recipients can result in donor germ cells-derived sperm production. this technique has the potential to be used as an alternative strategy for producing transgenic livestock with higher efficiency and less time and capital requirement than the current methods. the objective of the present study was to investigate whether the homologous transplantation of isolated buffalo germ cells could r ... | 2016 | 28278995 |
| 121 three-dimensional assessment of early corpus luteum vascularity in buffalo (bubalus bubalis). | the aim of the study was to develop an objective method to assess the vascular flow to the early corpus luteum (cl) in buffaloes using colour doppler ultrasound data. our hypothesis was that 3-dimensional (3d) volumetric analysis of vascularity would demonstrate lower variability between animals compared with conventional 2-dimensional (2d) analysis of single images. wave emergence and ovulation was synchronized in buffalo (n=16) using prostaglandin-gnrh based protocols. colour doppler ultrasono ... | 2016 | 28278918 |
| the effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using mehsani buffalo (bubalus bubalis). | in developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. it is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. a metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic mehsani buffalo (bubalus bubalis) fed with varying proportions of roughage. the ex ... | 2016 | 26711277 |
| amnion epithelial cells of buffalo (bubalus bubalis) term placenta expressed embryonic stem cells markers and differentiated into cells of neurogenic lineage in vitro. | aim of the present study was the isolation, culture, and characterization of amniotic membrane-derived epithelial cells (ae) from term placenta collected postpartum in buffalo. we found that cultured cells were of polygonal in shape, resistance to trypsin digestion and expressed cytokeratin-18 indicating that they were of epithelial origin. these cells have negative expression of mesenchymal stem cell markers (cd29, cd44, and cd105) and positive for pluripotency marker (oct4) genes indicated tha ... | 2016 | 26670951 |
| differential developmental competence and gene expression patterns in buffalo (bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells. | the developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (bubalus bubalis) nuclear transfer (nt) embryos from fetal fibroblasts (ffs), amnion mesenchymal stem cells (amscs) and in vitro fertilized (ivf) embryos were compared in the present studies. the in vitro expanded buffalo ffs showed a typical "s" shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. the in vitro cultured undifferentiated amscs showed a doubling t ... | 2016 | 26660476 |
| trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in nili ravi buffaloes (bubalus bubalis). | our objectives were to study the effect of trehalose in extender on (1) antioxidant enzymes profile during cryopreservation (after dilution, before freezing, and after thawing), (2) in vitro quality (after thawing), and (3) in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 20) from four buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60 mm) and frozen in french straws. at ... | 2016 | 26653954 |
| dispositions of enrofloxacin and its major metabolite ciprofloxacin in thai swamp buffaloes. | given the limited information available in this species, the aim of this study was to investigate the pharmacokinetic characteristics of enrofloxacin (er) and its major metabolite ciprofloxacin (cp) in buffaloes, bubalus bubalis. er was administered intravenously (i.v.) or subcutaneously (s.c.) to buffaloes at doses of 5.0 and 7.5 mg/kg bw, and plasma, urine and fecal samples were collected until 48 hr post-administration. the concentrations of er and cp in the plasma, urine and feces were analy ... | 2016 | 26596287 |
| supplementation of slow-release melatonin improves recovery of ovarian cyclicity and conception in summer anoestrous buffaloes (bubalus bubalis). | the role of melatonin as a protective neurohormone against restoring cyclicity in summer anoestrous animals in photoperiod species has gained wider acceptance. this study was designed to uncover the evidence the slow-release melatonin (mlt) has on initiation of ovarian cyclicity and conception rate (cr) in summer anoestrous buffaloes. thus, buffaloes diagnosed as summer anoestrous (absence of overt signs of oestrus, concurrent rectal examination and radioimmunoassay for serum progesterone at 10 ... | 2016 | 26566713 |
| retracted: bone morphogenetic protein 4 (bmp4) induces buffalo (bubalus bubalis) embryonic stem cell differentiation into germ cells. | the aim of the present study was to investigate the effect of bone morphogenetic protein 4 (bmp4) stimulation on differentiation of buffalo embryonic stem (es) cells into germ lineage cells. es cells were subjected to in vitro differentiation in floating and adherent cultures, under different bmp4 concentrations (20, 50 and 100 ngml(-1)) for different culture intervals (4, 8 and 14 days). qpcr analysis revealed that bmp4 at a concentration of 50-100 ngml(-1) for a culture period of 14 days led t ... | 2015 | 26529649 |
| optixcell improves the postthaw quality and fertility of buffalo bull sperm. | the present study was conducted to compare the liposome-containing, animal protein-free, commercially available optixcell extender with the tris-citric-egg yolk extender for postthaw quality and fertility of buffalo semen. semen was collected from five adult nili-ravi buffalo (bubalus bubalis) bulls of similar age group with an artificial vagina (at 42 °c) for 3 weeks (replicates). semen ejaculates from each buffalo bull were divided into two aliquots and diluted (at 37 °c having 50 × 10(6) sper ... | 2016 | 26522702 |
| morphologic and genotypic characterization of psoroptes mites from water buffaloes in egypt. | species delimitation of psoroptes spp. and identity of the parasite in water buffaloes remain poorly defined. in this study, psoroptes infestation on three water buffalo farms in egypt was examined based on morphometric characteristics, especially the opisthosomal setae of adult male mites. clinical investigations showed that 28% (196/700) of the sampled animals had mange infestation. microscopic examinations of 80 skin scrapings indicated the occurrence of psoroptes mites in 17 (21.3%) samples, ... | 2015 | 26517834 |
| actin polymerization: an event regulated by tyrosine phosphorylation during buffalo sperm capacitation. | in the female reproductive tract, the spermatozoa undergo a series of physiological and biochemical changes, prior to gaining the ability to fertilize, that result to capacitation. however, the actin polymerization and protein tyrosine phosphorylation are the two necessary steps for capacitation. in this study, we have demonstrated the actin polymerization and established the correlation between protein tyrosine phosphorylation and actin reorganization during in vitro capacitation in buffalo (bu ... | 2015 | 26514336 |
| buffalo (bubalus bubalis) scnt embryos produced from somatic cells isolated from frozen-thawed semen: effect of trichostatin a on the in vitro and in vivo developmental potential, quality and epigenetic status. | this study examined the effects of trichostatin a (tsa) treatment of reconstructed buffalo embryos, produced by hand-made cloning using somatic cells isolated from over a decade old frozen-thawed semen, on their in vitro and in vivo developmental competence, quality and epigenetic status. following treatment of reconstructed embryos with tsa (0, 50 or 75 nm) for 10 h prior to culture, the cleavage (100.0 ± 0, 94.5 ± 2.3 and 96.1 ± 1.2%, respectively) and blastocyst rate (50.6 ± 2.3, 48.4 ± 2.7 a ... | 2016 | 26503476 |
| impact of buserelin acetate or hcg administration on day 5 post-ovulation on subsequent luteal profile and conception rate in murrah buffalo (bubalus bubalis). | the present study aimed to establish the impact of buserelin acetate or hcg administration on day 5 post-ovulation on subsequent luteal profile and conception rate in buffalo. the buffalo (n=45) were subjected to an estrous synchronization protocol (synthetic analog of pgf2α administered, through intramuscular route, 11 days apart), followed by artificial insemination (ai) during mid to late estrus. on day 5 post-ovulation, buffalo were administered (i.m.) normal saline (control, n=14), busereli ... | 2015 | 26471839 |
| molecular characterization of t-cell immunoglobulin mucin domain-3 and galectin-9 genes of swamp- and riverine-type water buffaloes. | molecular characterization of t-cell immunoglobulin mucin domain-3 (tim-3) and galectin-9 (gal-9) genes of swamp- and riverine-type water buffaloes was conducted to compare these genes with other species; determine the unique characteristic specific in water buffalo; and provide baseline information for the assessment of disease progression in buffalo species. tim-3 and gal-9 genes were amplified, purified, sequenced and characterized. the sequence result of tim-3 in both types of water buffaloe ... | 2015 | 26441033 |
| molecular characterization of interferon regulatory factor 1 in bubalus bubalis. | interferon regulatory factor 1 (irf1) is functionally diverse in the regulation of immune response and is considered to be an important candidate gene for studying disease susceptibility in mammals. in this paper, we characterized the whole sequence of the irf1 gene in river buffalo (bubalus bubalis) and compared genomic and the amino acid sequences between different species. the buffalo irf1 gene was 7099 bp long and organized into 10 exons and nine introns. its molecular structure showed exact ... | 2015 | 26400319 |
| effect of relaxin on fertility parameters of frozen-thawed buffalo (bubalus bubalis) sperm. | the aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. sperm viability, motility, capacitation and the effect of relaxin on the fertilizing ability after heterologous ivf were evaluated. although viability was not affected, relaxin increased (p ... | 2015 | 26372314 |
| correction: correction: genetic evaluation of dual-purpose buffaloes (bubalus bubalis) in colombia using principal component analysis. | | 2015 | 26367259 |
| comparison of non-linear models to describe the lactation curves for milk yield and composition in buffaloes (bubalus bubalis). | in order to describe the lactation curves of milk yield (my) and composition in buffaloes, seven non-linear mathematical equations (wood, dhanoa, sikka, nelder, brody, dijkstra and rook) were used. data were 116,117 test-day records for my, fat (fp) and protein (pp) percentages of milk from the first three lactations of buffaloes which were collected from 893 herds in the period from 1992 to 2012 by the animal breeding center of iran. each model was fitted to monthly production records of dairy ... | 2016 | 26354679 |
| handmade cloned buffalo (bubalus bubalis) embryos produced from somatic cells isolated from milk and ear skin differ in their developmental competence, epigenetic status, and gene expression. | we compared the cloning efficiency of buffalo embryos produced by handmade cloning (hmc) using ear skin- and milk-derived donor cells. the blastocyst rate was lower (p < 0.05) for milk-derived than that for skin-derived embryos, whereas the total cell number and apoptotic index were similar. the global level of h3k9ac was higher (p < 0.05) in skin- than in milk-derived cells, whereas the level of h3k27me3 was similar in the two groups. the global level of h3k9ac was similar between milk-derived ... | 2015 | 26332294 |
| correction: genetic evaluation of dual-purpose buffaloes (bubalus bubalis) in colombia using principal component analysis. | | 2015 | 26312483 |
| evaluation of lipid peroxidation and antioxidant status on fenvalerate, nitrate and their co-exposure in bubalus bubalis. | the toxic effects of pesticides and minerals have been explored in different species, but still there is paucity of information regarding their combined toxicological effects. the present investigation reports oxidative stress induced by oral subacute exposure to fenvalerate (1 mg/kg) and sodium nitrate (20 mg/kg) alone, as well as in combination daily for 21 days in buffalo calves. fenvalerate exposure produced significant elevation in lipid peroxidation (lpo), glutathione peroxidase (gpx), whi ... | 2015 | 26267048 |
| glycome characterization of immunoglobulin g from buffalo (bubalus bubalis) colostrum. | immunoglobulin g (igg) is a major glycoprotein in ruminant colostrum. first day buffalo colostrum protein was purified on sephadex g-100 and its mass was determined by maldi-tof as 147.848 kda. the pmf data of protein subunits revealed its homology to igg, which was supported by the identification of peptide sequences lliygatsr and vyneylpapivr corresponding to light and heavy chains of igg by cid ms/ms analysis. the n-glycan microheterogeneity was established based on chemoselective glycoblotti ... | 2015 | 26239923 |
| [analysis and identification of water soluble components of water buffalo horn]. | this study is to analyze and identify the water soluble components of water buffalo horn (bubali cornu, wbh), and also establish a method for investigating these components. shotgun proteomic analysis identified proteins in wbh aqueous extraction: keratin, collagen, desmoglein, etc. ultrafiltration and lc-ms/ms were used to separate and identify the peptides in wbh aqueous extract, as a result, identified peptides were mainly derived from nonspecific degradation products of keratin and collagen, ... | 2015 | 26234143 |
| genetic evaluation of dual-purpose buffaloes (bubalus bubalis) in colombia using principal component analysis. | genealogy and productive information of 48621 dual-purpose buffaloes born in colombia between years 1996 and 2014 was used. the following traits were assessed using one-trait models: milk yield at 270 days (my270), age at first calving (afc), weaning weight (ww), and weights at the following ages: first year (w12), 18 months (w18), and 2 years (w24). direct additive genetic and residual random effects were included in all the traits. maternal permanent environmental and maternal additive genetic ... | 2015 | 26230093 |
| metaphylactic treatment strategies with toltrazuril and diclazuril and growth performance of buffalo calves exposed to a natural eimeria infection. | five controlled field trials were conducted in southern italy to evaluate the effect of metaphylactic treatment strategies of toltrazuril and diclazuril for the control of coccidiosis in water buffaloes naturally infected by eimeria spp. the 5 farms were divided into two types (a and b) according to their management system (individual or collective breeding of buffalo calves). in the farms of type a (no. 3), the buffalo calves were bred in individual boxes from the birth to the 7th/8th week of a ... | 2015 | 26215929 |
| optimization of buffalo (bubalus bubalis) embryonic stem cell culture system. | in order to retain an undifferentiated pluripotent state, embryonic stem (es) cells have to be cultured on feeder cell layers. however, use of feeder layers limits stem cell research, since experimental data may result from a combined es cell and feeder cell response to various stimuli. | 2015 | 26199905 |
| potential risk factors associated with ill-thrift in buffalo calves (bubalus bubalis) raised at smallholder farms in egypt. | failure to grow (ill-thrift) in calves has a negative effect on animal production and health. the present study was carried out from november, 2009 to may, 2013 to investigate the risk factors of ill-thrift in buffalo calves. a total of 344 calves at 78 smallholder farms were selected randomly. a questionnaire was designed to include managemental, nutritional and disease risk factors. serum selenium, copper, zinc, iron, calcium, phosphorus and magnesium were measured. data were subjected to logi ... | 2015 | 26199751 |
| characterization of arcobacter suis isolated from water buffalo (bubalus bubalis) milk. | during a survey in a dairy plant in italy, the second strain (strain fg 206) of arcobacter suis described in the literature was isolated from raw water buffalo milk. the objective of this study was to confirm the species identification, better define the species by comparing its characteristics with those of the reference strain (f41(t) = cect 7833(t) = lmg 26152(t)) and to investigate its potential clinical relevance by detecting the virulence gene pattern of the new strain. phenotypical charac ... | 2015 | 26187844 |
| water buffalo as sentinel animals for schistosomiasis surveillance. | | 2015 | 26170512 |
| molecular characterization and expression of buffalo (bubalus bubalis) dead-box family vasa gene and mrna transcript variants isolated from testis tissue. | vasa is a member of the dead-box protein family that plays an indispensable role in mammalian spermatogenesis, particularly during meiosis. in the present study, we isolated, sequenced, and characterized vasa gene in buffalo testis. here, we demonstrated that vasa mrna is expressed as multiple isoforms and uses four alternative transcriptional start sites (tsss) and four different polyadenylation sites. the tsss identified by 5'-rna ligase-mediated rapid amplification of cdna ends (rlm-5'-race) ... | 2015 | 26127001 |
| cross talk between kgf and kitlg proteins implicated with ovarian folliculogenesis in buffalo bubalus bubalis. | molecular interactions between mesenchymal-derived keratinocyte growth factor (kgf) and kit ligand (kitlg) are essential for follicular development. these factors are expressed by theca and granulosa cells. we determined full length coding sequence of buffalo kgf and kitlg proteins having 194 and 274 amino acids, respectively. the recombinant kgf and kitlg proteins were solubilized in 10 mm tris, ph 7.5 and 50 mm tris, ph 7.4 and purified using ni-nta column and gst affinity chromatography, resp ... | 2015 | 26083339 |
| liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (bubalus bubalis) semen. | demand for alternative of egg yolk in freezing extenders have increased in recent years due to variability in egg yolk composition, risk of microbial contamination and presence of steroid hormones. the alternative to egg yolk-based extender (ey) can be soya lecithin-based extender (sl) and liposome-based extender (lp). however, the efficacy of sl is still a matter of debate. few studies have been performed on the effect of lp but to date evaluation of buffalo semen cryopreserved in lp has not be ... | 2015 | 26065809 |
| establishment of trophectoderm cell lines from buffalo (bubalus bubalis) embryos of different sources and examination of in vitro developmental competence, quality, epigenetic status and gene expression in cloned embryos derived from them. | despite being successfully used to produce live offspring in many species, somatic cell nuclear transfer (nt) has had a limited applicability due to very low (>1%) live birth rate because of a high incidence of pregnancy failure, which is mainly due to placental dysfunction. since this may be due to abnormalities in the trophectoderm (te) cell lineage, te cells can be a model to understand the placental growth disorders seen after nt. we isolated and characterized buffalo te cells from blastocys ... | 2015 | 26053554 |
| production of a cloned buffalo (bubalus bubalis) calf from somatic cells isolated from urine. | this study was aimed at isolation of cells from urine and skin on the ventral part of the tails of healthy adult female buffaloes (bubalus bubalis), an area rarely exposed to solar radiation, establishment of the cells in culture, and their use as donor cells for production of buffalo embryos by handmade cloning (hmc). the blastocyst rate and total cell number of urine- and tail skin-derived embryos were similar to those of control embryos derived from ear skin cells; however, their apoptotic in ... | 2015 | 26053516 |
| effect of pour-on alphacypermethrin on feed intake, body condition score, milk yield, pregnancy rates, and calving-to-conception interval in buffaloes. | the aims of this study were to assess the efficacy of alphacypermethrin (acyp) on pediculosis due to haematopinus tuberculatus and to evaluate the influence of the treatment on productive and reproductive performance in buffaloes (bubalus bubalis) reared in an intensive system. the trial was performed on 56 pluriparous buffaloes at 86.8 ± 8.1 d in milk. the animals underwent individual louse count and were divided into 2 homogenous groups according to louse count, age, number of lactations, days ... | 2015 | 26020206 |
| buffalo (bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro. | recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. the aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. we have observed that both presumptive epith ... | 2015 | 26019121 |
| chromium supplements in the feed for lactating murrah buffaloes (bubalus bubalis): influence on nutrient utilization, lactation performance, and metabolic responses. | this study was conducted to evaluate the effect of different levels of inorganic chromium (cr) on nutrient intake, nutrient utilization, milk mineral contents, and blood biochemical in lactating murrah buffaloes. twenty-four multifarious murrah buffaloes were blocked into four groups having six animals in each group and fed for 150 days post-partum. feeding regimen was same in all the groups except that treatment groups were supplemented with 0.0, 0.5, 1.0, and 1.5 mg inorganic cr/kg dry matter ... | 2015 | 26013395 |
| isolation, characterization and functional analysis of full length p53 cdna from bubalus bubalis. | p53 plays a pivotal role in maintaining the genomic integrity of the cell and has an important role in cellular transformation. we isolated and cloned a full length p53 cdna (bp53) from water buffalo in expression vectors designed to generate tagged proteins with flag or gfp. bp53 was found to be 1161 nucleotide long and codes for 386 amino acid residues with 79% homology with human p53 containing 393 amino acids. although bp53 has some inherent differences in amino acid composition in different ... | 2015 | 26003295 |
| localization of igf proteins in various stages of ovarian follicular development and modulatory role of igf-i on granulosa cell steroid production in water buffalo (bubalus bubalis). | the present study aimed to determine the expression of insulin like growth factor (igf) genes in the bubaline ovarian follicles and modulatory role of igf-i on progesterone production from granulosa cells (gc) of pre-ovulatory follicle in vitro. according to size, follicles were classified into four groups: gi (small), gii (medium), giii (large) and giv (preovulatory). all igf genes were expressed in both gc and theca interna (ti) cells. the relative expression of igf-i and igf receptor i (igfr- ... | 2015 | 25951771 |
| relationship between seminal plasma tuberoinfundibular peptide of 39 residues and sperm functional attributes in buffalo (bubalus bubalis). | the buffalo seminal plasma protein profile and its relationship with sperm quality have not been studied in detail. thus, the aim of the present study was to profile buffalo seminal plasma proteins and to assess the relationship between differentially expressed proteins and sperm characteristics. semen samples (n = 44) were collected from 11 murrah buffalo bulls (four ejaculates from each animal) and seminal plasma protein profiling was performed by sodium dodecyl sulfate-polyacrylamide gel elec ... | 2015 | 25940576 |
| efficiency of antifreeze glycoproteins for cryopreservation of nili-ravi (bubalus bubalis) buffalo bull sperm. | experiments were conducted to evaluate the effect of antarctic fish antifreeze glycoproteins, (afgp) size 1-5 (34-10.5 kda) and 7-8 (3.2 and 2.4 kda) in extender on buffalo bull sperm at cooling (4 °c) and at post thawing. semen was collected from three nili-ravi buffalo bulls with artificial vagina for 3 weeks. qualifying ejaculates from each buffalo bull were diluted (at 37 °c having 50×10(6) sperm/ml) in tris-citric acid extender containing afgp at 0 (control), 0.1, 1 and 10 μg/ml. an aliquot ... | 2015 | 25863987 |
| downregulation of dna methyltransferase 1 in zona-free cloned buffalo (bubalus bubalis) embryos by small interefering rna improves in vitro development but does not alter dna methylation level. | aberrant epigenetic reprogramming, especially genomic hypermethylation, is implicated as the primary reason behind the failure of the cloning process during somatic cell nuclear transfer (scnt). we transfected one-cell-stage zona-free buffalo embryos produced by handmade cloning with 50 nm dnmt1 small interfering rna (sirna), using lipofectamine, to knockdown the dna methyltransferase 1 (dnmt1) gene. sirna treatment decreased (p<0.001) the expression level of dnmt1 mrna and dnmt1 protein in the ... | 2015 | 25826721 |
| sustained delivery of exogenous melatonin influences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (bubalus bubalis). | high ambient temperature during summer in tropical and subtropical countries predisposes water buffaloes (bubalus bubalis) to develop oxidative stress having antigonadotropic and antisteroidogenic actions. melatonin is a regulator of seasonal reproduction in photoperiodic species and highly effective antioxidant and free radical scavenger. therefore, a study was designed to evaluate the effect of sustained-release melatonin on biomarkers of oxidative stress i.e., the serum malondialdehyde (mda) ... | 2015 | 25805692 |
| evaluation of thermal comfort, physiological, hematological, and seminal features of buffalo bulls in an artificial insemination station in a tropical environment. | this study aimed to assess the variation over time in thermal comfort indices and the behavior of physiological parameters related to thermolysis, blood parameters, and semen in natura of buffalo bulls reared in tropical climate. the study was carried out in an artificial insemination station under a humid tropical climate (afi according to köppen). ten water buffalo bulls (bubalus bubalis) were used during the 5 months (april to august) of study. the environmental temperature humidity index (th ... | 2015 | 25801015 |
| effect of donor cell type on developmental competence, quality, gene expression, and epigenetic status of interspecies cloned embryos produced using cells from wild buffalo and oocytes from domestic buffalo. | this study compared the cloning efficiency of donor cells of fibroblast and epithelial origin isolated from ear skin of a wild buffalo (bubalus arnee) and used with cytoplasts from domestic buffalo (bubalus bubalis) in interspecies scnt by hand-made cloning. the cleavage (93.0 ± 2.8% vs. 85.6 ± 2.4%) and blastocyst rates (50.6 ± 4.0% vs. 20.5 ± 2.6%) were higher (p < 0.05) for fibroblasts than those for epithelial cells, whereas the total cell number (490 ± 42 and 492 ± 95, respectively) and apo ... | 2015 | 25799471 |
| comparative genomic analysis of buffalo (bubalus bubalis) nod1 and nod2 receptors and their functional role in in-vitro cellular immune response. | nucleotide binding and oligomerization domain (nod)-like receptors (nlrs) are innate immune receptors that recognize bacterial cell wall components and initiate host immune response. structure and function of nlrs have been well studied in human and mice, but little information exists on genetic composition and role of these receptors in innate immune system of water buffalo--a species known for its exceptional disease resistance. here, a comparative study on the functional domains of nod1 and n ... | 2015 | 25786158 |
| genetic analysis of river, swamp and hybrid buffaloes of north-east india throw new light on phylogeography of water buffalo (bubalus bubalis). | this study analysed buffaloes from north-east india and compared their nuclear and mitochondrial dna variations with buffaloes of mainland india, china, mediterranean and south-east asia. microsatellite genotypes of 338 buffaloes including 210 from six north-east indian buffalo populations and three mainland indian breeds were analysed to evaluate their genetic structure and evolutionary relationships. phylogenetic analysis and multidimensional scaling plot of pairwise fst revealed the clusterin ... | 2015 | 25780854 |
| dietary inorganic chromium in summer-exposed buffalo calves (bubalus bubalis): effects on biomarkers of heat stress, immune status, and endocrine variables. | the aim of this study was to evaluate the effect of different levels of inorganic chromium (cr) on heat stress, immune response, and hormonal variation in murrah buffalo calves during the summer season. twenty-four growing murrah buffalo calves were randomly allocated into four treatments for a period of 120 days. feeding regimen was same in all the groups, except the buffalo calves in treatment groups were additionally supplemented with 0.5, 1.0, and 1.5 mg of inorganic cr/kg dry matter. buffal ... | 2015 | 25762098 |
| polymorphisms in oxytocin and α1a adrenergic receptor genes and their effects on production traits in dairy buffaloes. | the use of molecular markers may auxiliary the buffalo breeding. the oxytocin (oxt) and the adrenergic receptor α1a (adra1a) may be involved in milk ejection in ruminants. the aim of this study was to verify the existence of polymorphisms in the oxt and adra1a genes and their associations with milk production traits. a total of 220 buffaloes were genotyped using pcr-rflp for both genes. the snp identified in the adra1a gene was associated with protein percentage in dairy buffaloes. this is the f ... | 2015 | 25695522 |
| isolation and functional characterization of buffalo (bubalus bubalis) β-casein promoter for driving mammary epithelial cell-specific gene expression. | therapeutic proteins are produced in microbes, mammalian cell lines, and body fluids by applying recombinant dna technology. they are required for compensating the deficiency of essential proteins in patients. animal bioreactors producing such valuable bio-pharmaceuticals in body fluids have lately emerged as efficient and cost-effective expression systems. promoters, along with other regulatory elements of genes coding for milk proteins, have been cloned from few species for directing the expre ... | 2015 | 25678138 |
| potential functional gene diversity involved in methanogenesis and methanogenic community structure in indian buffalo (bubalus bubalis) rumen. | understanding the methanogen community structure and methanogenesis from bubalus bubalis in india may be beneficial to methane mitigation. our current understanding of the microbial processes leading to methane production is incomplete, and further advancement in the knowledge of methanogenesis pathways would provide means to manipulate its emission in the future. in the present study, we evaluated the methanogenic community structure in the rumen as well as their potential genes involved in met ... | 2015 | 25663664 |
| phenotypic characterization and multivariate analysis to explain body conformation in lesser known buffalo (bubalus bubalis) from north india. | phenotypic characterization and body biometric in 13 traits (height at withers, body length, chest girth, paunch girth, ear length, tail length, length of tail up to switch, face length, face width, horn length, circumference of horn at base, distances between pin bone and hip bone) were recorded in 233 adult gojri buffaloes from punjab and himachal pradesh states of india. traits were analysed by using varimax rotated principal component analysis (pca) with kaiser normalization to explain body ... | 2015 | 25656215 |
| early cleavage of handmade cloned buffalo (bubalus bubalis) embryos is an indicator of their developmental competence and quality. | following ivf, embryos which cleave early have been shown to have higher developmental competence and quality than those that cleave relatively later across many species. we investigated the effect of time of cleavage on the developmental competence, quality, epigenetic status and gene expression in buffalo embryos produced by handmade cloning (hmc). following classification of embryos as early cleaving (ec) or late cleaving (lc) based on whether they had cleaved or not at 24 h post in vitro cul ... | 2015 | 25604613 |
| acui identifies water buffalo csn3 genotypes by rflp analysis. | | 2014 | 25572073 |
| reproductive characteristics and thyroidal function in relation with season in khuzestan buffalo (bubalus bubalis) bulls. | high ambient temperature is the major constraint on buffalo productivity. the aim of this study was to evaluate the reproductive performance and thyroid gland function in winter and summer seasons in khuzestan buffalo bulls. six male indigenous buffaloes of khuzestan with nearly the same age (2-3 years old) and weight were used. semen and blood samples through jugular vein were collected, every two weeks throughout the summer and winter seasons. the thyroid hormones and thyrotropin stimulating h ... | 2014 | 25568719 |
| comparative efficacy of e-17β and gnrh administration on day 0 of a controlled internal drug release (cidr) based protocol on synchrony of wave emergence, ovulation and conception rates in murrah buffalos (bubalus bubalis). | the study was undertaken to compare the efficacy of an estradiol-17β + cidr based protocol with the conventional ovsynch + cidr based protocol for synchrony of wave emergence and ovulation in murrah buffalos. in group i (n=25), on day 0 (beginning of experiment), buffaloes were administered a cidr device (1.38 g p4) and concurrently received 1.5 mg e-17β. on day 9, the cidr was removed and a prostaglandin (pg) f2α analogue (500 µg) was administered. on day 11, buffaloes were administered a gonad ... | 2015 | 27175151 |
| sequence characterization and comparative analysis of the gastrotropin gene in buffalo (bubalus bubalis). | in this study, we compared the complete sequence of the fabp6 gene from an animal representing the murrah breed of the river buffalo (bubalus bubalis) with the gene sequence from different mammals. the buffalo fabp6 gene is 6105 bp in length and is organized into four exons (67, 176, 90, and 54 bp), three introns (1167, 1737, and 2649 bp), a 5ꞌutr (93 bp), and a 3ꞌutr (72 bp). a total of 22 repetitive elements were identified at the intronic level, and four of these (l1mc, l1m5, mirb, and charli ... | 2014 | 25526214 |
| a comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (bubalus bubalis) embryos. | the efficiency of two cell types, namely adult fibroblasts, and amniotic fluid stem (afs) cells as nuclear donor cells for somatic cell nuclear transfer by hand-made cloning in buffalo (bubalus bubalis) was compared. the in vitro expanded buffalo adult fibroblast cells showed a typical "s" shape growth curve with a doubling time of 40.8 h and stained positive for vimentin. the in vitro cultured undifferentiated afs cells showed a doubling time of 33.2 h and stained positive for alkaline phosphat ... | 2016 | 25501536 |
| igf-1 attenuates lps induced pro-inflammatory cytokines expression in buffalo (bubalus bubalis) granulosa cells. | interaction between immune and endocrine system is a diverse process influencing cellular function and homeostasis in animals. negative energy balance (neb) during postpartum period in dairy animals usually suppresses these systems resulting in reproductive tract infection and infertility. these negative effects could be due to competition among endocrine and immune signaling pathways for common signaling molecules. the present work studied the effect of igf-1 (50 ng/ml) on lps (1 μg/ml) mediate ... | 2015 | 25433435 |
| characterization of leptin receptor gene in bubalus bubalis and association analysis with body measurement traits. | leptin has a pleiotropic effect on regulating appetite, energy metabolism, growth, reproduction, body composition and immunity. this property supports leptin and its receptor as candidate genes for evaluating genetic polymorphisms to associate with growth, milk yield and other economic traits. the aim of this study is to characterize the leptin receptor gene in bubalus bubalis, to identify single-nucleotide polymorphism (snp) sites in different coding and non-coding regions and to analyse potent ... | 2015 | 25431006 |
| treatment of buffalo (bubalus bubalis) donor cells with trichostatin a and 5-aza-2'-deoxycytidine alters their growth characteristics, gene expression and epigenetic status and improves the in vitro developmental competence, quality and epigenetic status of cloned embryos. | we examined the effects of treating buffalo skin fibroblast donor cells with trichostatin a (tsa), a histone deacetylase (hdac) inhibitor, and 5-aza-2'-deoxycytidine (5azadc), a dna methyltransferase (dnmt) inhibitor, on the cells and embryos produced by hand-made cloning. treatment of donor cells with tsa or 5azadc resulted in altered expression levels of the hdac1, dnmt1, dnmt3a, p53, caspase3 and caspase9 genes and global levels of acetylation of lysine at position 9 or 14 in histone 3 (h3k9/ ... | 2016 | 25409339 |
| effect of dietary protein on intake, nutrients utilization, nitrogen balance, blood metabolites, growth and puberty in growing bhadawari buffalo (bubalus bubalis) heifers. | fifteen bhadawari buffalo heifers of 207 ± 9.78 kg mean body weight were randomly distributed into three dietary groups to evaluate the effect of protein level on nutrient utilization, nitrogen (n) balance, growth rate, blood metabolites, and puberty. all animals were offered wheat straw-berseem diets supplemented with concentrate mixtures of similar energy (2.7 mcal/kg) and different protein levels (14.3-22%). animals of standard-protein group (spg) were offered protein and energy as per requir ... | 2015 | 25407740 |
| effect of additional chromium supplementation on health status, metabolic responses, and performance traits in periparturient murrah buffaloes (bubalus bubalis). | this study was conducted to determine the effects of inorganic chromium (cr) on body condition, metabolic responses, lactation performance, and reproductive parameters in periparturient murrah buffaloes. twenty-four multiparous advanced pregnant murrah buffaloes were randomly assigned to four treatment diets. feeding regimen was the same in all the groups, except that the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of cr/kg dry matter (dm) ... | 2015 | 25388756 |
| assessment of genetic variability and structuring of riverine buffalo population (bubalus bubalis) of indo-gangetic basin. | the buffalo population of uttar pradesh (up) constitutes 26.1% of the total buffalo population of india, yet this population has not been classified into distinct breeds or subpopulations due to lack of systematic study. genetic variation at 30 microsatellite loci was examined and statistical analysis was carried out to reveal genetic diversity, demographic parameters of these buffaloes and to investigate the existence of population substructures underlying geographical distribution. the mean nu ... | 2015 | 25380468 |
| expression of mrna encoding igf-i, igf-ii, type-i,and ii igf-receptors and igf-binding proteins-1-4 during ovarian follicular development in buffalo (bubalus bubalis). | the present study was designed to investigate the expression pattern of igf-i, igf-ii, type-i and ii igf-receptors, and igfbp-1-4 in different stages of buffalo ovarian preantral follicles (pfs), antral follicles (afs), ovulatory follicles (ofs), and immature (im) and in vitro matured (mo) oocytes. buffalo ovaries were collected from local abattoir, pfs (200-250 µm), afs (1-3 mm), and ofs (5-8 mm) were isolated by mechanical method. pfs, afs, ofs, and oocytes were lysed to release mrna, reverse ... | 2015 | 25380459 |
| effects of in vitro zinc sulphate additive to the semen extender on water buffalo (bubalusbubalis) spermatozoa before and after freezing. | the objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and dna stability) after cryopreservation. | 2014 | 25379162 |
| assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes. | the present study was designed to investigate the sperm damages occurring in acrosome, plasma membrane, mitochondrial activity, and dna of fresh, equilibrated and frozen-thawed buffalo semen by fluorescent probes. the stability of sperm acrosome and plasma membrane stability, mitochondrial activity and dna status were assessed by fluorescein conjugated lectin pisum sativum agglutinin, annexin-v/propidium iodide, jc-1 and tunel assay, respectively, under the fluorescent microscope. the damages pe ... | 2016 | 25373338 |
| the effect of buffalo cd14 shrna on the gene expression of tlr4 signal pathway in buffalo monocyte/macrophages. | cd14 plays a crucial role in the inflammatory response to lipopolysaccharide (lps), which interacts with tlr4 and md-2 to enable cell activation, resulting in inflammation. upstream inhibition of the inflammation pathway mediated by bacterial lps, toll-like receptor 4 (tlr4) and cluster of differentiation antigen 14 (cd14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. to explore the effect of cd14 downregulation on the expression of tlr4 signaling ... | 2014 | 25355240 |
| expression and localization of aquaporin-1 along the intestine of colostrum suckling buffalo calves. | aquaporin-1 (aqp1), a six-transmembrane domain protein, belongs to a highly conserved group of proteins called aquaporins known to regulate permeability across cell membranes. although the role of aqp1 has been extensively studied, its specific activity along the gastrointestinal tract in animals during early postnatal development is poorly known. this study investigates the expression of aqp1 mrna and protein in the small and large intestine of water buffalo calves after colostrum ingestion usi ... | 2015 | 25348329 |
| expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (bubalus bubalis) and the effect of insulin-like growth factor i on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro. | this study investigated the expression and localization of insulin-like growth factor (igf) system at different stages of buffalo cl and the role of igf-i in stimulating vascular endothelial growth factor (vegf) and progesterone (p4) production in cultured luteal cells. the mrna expression of igf system, vegf, steroidogenic acute regulatory protein, p450scc, and hydroxysteroid dehydrogenase (hsd) was investigated by quantitative real-time polymerase chain reaction (pcr). protein expression of ig ... | 2015 | 25304995 |
| identification of pregnancy-associated glycoproteins by peptide mass fingerprinting in water buffalo (bubalus bubalis). | ruminant placentas synthesize pregnancy-associated glycoproteins (pags) during pregnancy, which serve as biomarkers of pregnancy. the present study was conducted to verify, whether pags are expressed in buffalo placenta by using lectin-based affinity chromatography and peptide mass finger printing (pmf). fetal cotyledonary tissues were collected from gravid uteri procured from slaughtered house. proteins were extracted and subjected to wheat germ agglutinin (wga) lectin affinity chromatography t ... | 2014 | 25296505 |
| morphology of the lingual papillae of egyptian buffalo (bubalus bubalis). | the mammalian tongue has different functions for feeding. we investigated the morphological characteristics of the surface of the tongue of egyptian buffalo (bubalus bubalis) using scanning electron microscopy. the lingual surface exhibited different degrees of specialization. the tongue can be divided into the apex, body, and root. the lingual prominence was observed on the posterior area of the lingual body. the lenticular, conical, and vallate papillae were observed on the caudal part of the ... | 2014 | 25274404 |
| seasonal variations in developmental competence and relative abundance of gene transcripts in buffalo (bubalus bubalis) oocytes. | hot season is a major constraint to production and reproduction in buffaloes. the present work aimed to investigate the effect of season on ovarian function, developmental competence, and the relative abundance of gene expression in buffalo oocytes. three experiments were conducted. in experiment 1, pairs of buffalo ovaries were collected during cold season (cs, autumn and winter) and hot season (hs, spring and summer), and the number of antral follicles was recorded. cumulus oocyte complexes (c ... | 2014 | 25156970 |
| role of linker histone h1c during the reprogramming of chinese swamp buffalo (bubalus bubalis) embryos produced by somatic cell nuclear transfer. | during reprogramming, there is exchange of histone h1c and the oocyte-specific linker histone, and h1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (scnt). the aim of the present study was to investigate the role of the h1c gene in scnt reprogramming in chinese swamp buffalo (bubalus bubalis) using rna interference (rnai). chinese swamp buffalo h1c gene sequences were obtained and h1c-rnai vectors were designed, synthesised and then transfect ... | 2016 | 25145348 |
| a comparison of flotac and cff techniques in detecting gastrointestinal parasites in water buffaloes (bubalus bubalis). | the objective of the study was to compare the usefulness of flotac and centrifugal fecal flotation (cff) techniques. more specifically, the taxonomic classes (nematoda and cestoda) of endoparasites present in fecal samples of buffaloes are identified, the sensitivity and specificity of flotac relative to cff are calculated, and the agreement of both techniques is evaluated using kappa statistics. fresh fecal samples from 220 buffaloes in 10 municipalities were collected. sheather's sugar was use ... | 2014 | 25115063 |
| 'soya milk tris-based phytoextender reduces apoptosis in cryopreserved buffalo (bubalus bubalis) spermatozoa'. | the objective of this study was to investigate the effect of newly developed soya milk tris (smt)-based phytoextender as an alternative to egg yolk tris (eyt) extender used for cryopreservation of buffalo (bubalus bubalis) spermatozoa on apoptosis. fresh buffalo semen (control without dilution) was cryopreserved in conventional eyt (20% egg yolk v/v in tris) and smt (25% soya milk v/v in tris) extender and used for the assessment of expression of apoptotic proteins. proteins extracted from a tot ... | 2014 | 25112668 |
| differential expression of homeobox c11 protein in water buffalo bubalus bubalis and its putative 3d structure. | the homeobox (hox) family complex contains 39 genes, clustered into four groups (a-d) all expressing in sequential manner. the hox proteins are transcriptional factors involved in regulation of pattern formation of the anterio-posterior body axis across the species. most of the hox family genes have been studied with respect to their organization and expression during the embryonic stages. however, expression pattern of homeobox c11 (hoxc11) gene in the 5' region, particularly in higher mammals ... | 2014 | 25080327 |
| quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (bubalus bubalis) embryos and in putative embryonic stem cells derived from them. | parthenogenetically produced embryos and embryonic stem (es) cells derived from them offer a unique model for investigating the role of transcription factors in embryonic genome activation (ega), pluripotent lineage specification and in pluripotency and self-renewal of es cells because of the unique nature of these embryos. there is little information on the quantitative expression of important genes in parthenogenetically produced embryos and in es cells derived from them. the present study exa ... | 2014 | 25077841 |
| efficiency to reach age of puberty and behaviour of buffalo heifers (bubalus bubalis) kept on pasture or in confinement. | in order to evaluate the influence of rearing system (free-ranging (fr) v. confinement (c)) on buffalo heifer efficiency to reach age of puberty and on behavioural and immune functions, two experiments were conducted from september 2010 to october 2011. in experiment i, 32 subjects aged 8 to 9 months at the start of experiment were used. a total of 16 animals (group c) were group housed in an indoor slatted floor pen (4 m2/animal) with an outdoor paddock (4 m2/animal); 16 others grazed on a medi ... | 2014 | 25076110 |
| carcass characteristics and meat quality of swamp buffaloes (bubalus bubalis) fattened at different feeding intensities. | twenty-four male 1-year old swamp buffaloes (bubalus bubalis) were randomly allocated to 4 groups. one group grazed on guinea grass (gg) and another on guinea grass and the legume stylosanthes guianensis (gl). the other two groups were kept in pens and fed freshly cut guinea grass and concentrate at an amount of 1.5% (gc1.5) and 2.0% (gc2.0) of body weight, respectively. the effect of the different feeding intensities on carcass characteristics and meat quality were assessed. the mean body weigh ... | 2014 | 25049987 |
| body condition, energy balance and immune status of periparturient murrah buffaloes (bubalus bubalis) supplemented with inorganic chromium. | periparturient murrah buffaloes were used to determine whether body condition, energy balance and immune status are affected by inorganic cr supplementation. twenty-four murrah buffaloes were blocked into four groups having six animals in each group and fed for 60 days pre-partum to 150 days post-partum. feeding regimen was same in all the groups except that these were supplemented with 0.0, 0.5, 1.0 and 1.5 of cr per kilogram of dry matter (dm) in the four respective groups. buffaloes were weig ... | 2014 | 25037066 |
| genome-wide profiling of sperm dna methylation in relation to buffalo (bubalus bubalis) bull fertility. | the dna methylation pattern in spermatozoa of buffalo bulls of different fertility status was investigated. spermatozoa isolated dna from two groups of buffalo bulls (n = 5), selected based on their artificial insemination-generated conception rate data followed by ivf efficiency, were studied for global methylation changes using a custom-designed 180 k buffalo (bubalus bubalis) cpg island/promoter microarray. a total of 96 individual genes with another 55 genes covered under cpg islands were fo ... | 2014 | 25023295 |
| insights into resistome and stress responses genes in bubalus bubalis rumen through metagenomic analysis. | buffalo rumen microbiota experience variety of diets and represents a huge reservoir of mobilome, resistome and stress responses. however, knowledge of metagenomic responses to such conditions is still rudimentary. we analyzed the metagenomes of buffalo rumen in the liquid and solid phase of the rumen biomaterial from river buffalo adapted to varying proportion of concentrate to green or dry roughages, using high-throughput sequencing to know the occurrence of antibiotics resistance genes, genet ... | 2014 | 24985977 |
| construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (bubalus bubalis) mammary epithelial cell line. | the aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hins) expression in transgenic buffalo for therapeutic use. we have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitro cultured buffalo mammary epithelial cells (bumecs). human pro-insulin coding region was isolated from human genomic dna by intron skipping pcr primer and furin cleavage site was i ... | 2014 | 24969480 |
| developmental competence and expression pattern of bubaline (bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro. | to determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5 °c) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. | 2014 | 24938361 |
| evaluation of antifreeze protein iii for cryopreservation of nili-ravi (bubalus bubalis) buffalo bull sperm. | lower fertility in buffaloes with frozen-thawed semen is attributed to sperm damage that is believed to be due to formation of ice crystals during freeze/thaw process. it was hypothesized that antifreeze proteins in the extender may improve the post thaw quality of buffalo bull sperm. for this purpose, two separate experiments were conducted to evaluate antifreeze proteins iii (afp iii) at 0 (control), 0.1, 1 and 10 μg/ml (experiment i) and 0 (control), 0.01, 0.1 and 1 μg/ml (experiment ii) for ... | 2014 | 24925471 |
| effects of trichostatin a on in vitro development and dna methylation level of the satellite i region of swamp buffalo (bubalus bubalis) cloned embryos. | trichostatin a (tsa), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several species. this brings our attention to investigation of the effects of tsa on developmental potential of swamp buffalo cloned embryos. swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp buffalo enucleated oocytes. after fusion, reconstructed oocytes were treated with 0, 25 or 50 nm tsa for 10 h. the results showed ... | 2014 | 24909601 |
| morphogenesis of mammary glands in buffalo (bubalus bubalis). | the present research was elucidated on the morphogenesis of mammary gland of buffalo during prenatal development. total of 16 foetuses ranging from 1.2 cm (34 days) to 108 cm cvrl (curved crown rump length) (317 days) were used for study. the study revealed that mammary line was first observed at 1.2 cm cvrl (34 days), mammary hillock at 1.7 cm (37 days), and mammary bud at 2.6 cm cvrl (41 days) foetuses. epidermal cone was found at 6.7 cm cvrl (58 days) whereas primary and secondary ducts were ... | 2014 | 24876967 |
| energy metabolites, lipid variables and lactation performance of periparturient murrah buffaloes (bubalus bubalis) fed on diet supplemented with inorganic chromium. | the aim of this study was to elucidate the effects of chromium (cr) supplementation as inorganic cr (crcl3·6h2o) on energy balance, lipid peroxidation, and lactation performance in periparturient murrah buffaloes. twenty-four multiparous murrah buffaloes according to lactation, parity, body mass, and expected calving date were divided equally. experimental buffaloes were randomly assigned to four treatment diets: a control diet and three diets with an inorganic cr supplementation at 0.5, 1.0, an ... | 2014 | 24833279 |