| antioxidant and nitrite-scavenging capacities of phenolic compounds from sugarcane (saccharum officinarum l.) tops. | sugarcane tops were extracted with 50% ethanol and fractionated by petroleum ether, ethyl acetate (etoac), and n-butyl alcohol successively. eight phenolic compounds in etoac extracts were purified through silica gel and sephadex lh-20 column chromatographies, and then identified by nuclear magnetic resonance and electrospray ionization mass spectra. the results showed that eight phenolic compounds from etoac extracts were identified as caffeic acid, cis-p-hydroxycinnamic acid, quercetin, apigen ... | 2014 | 25162956 |
| variation in chilling tolerance for photosynthesis and leaf extension growth among genotypes related to the c4 grass miscanthus ×giganteus. | the goal of this study was to identify cold-tolerant genotypes within two species of miscanthus related to the exceptionally chilling-tolerant c4 biomass crop accession: m. ×giganteus 'illinois' (mxg) as well as in other mxg genotypes. the ratio of leaf elongation at 10 °c/5 °c to that at 25 °c/25 °c was used to identify initially the 13 most promising miscanthus genotypes out of 51 studied. net leaf co2 uptake (a sat) and the maximum operating efficiency of photosystem ii (фpsii) were measured ... | 2014 | 25039073 |
| comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-pcr. | the increasingly used real time quantitative reverse transcription-pcr (qrt-pcr) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). in order to facilitate gene expression studies in sugarcane (saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate reference genes was evaluated. the genorm, normfinder and deltact methods were used for selecting stably expressed internal controls across ... | 2014 | 24823940 |
| extraction optimization by response surface methodology: purification and characterization of phytosterol from sugarcane (saccharum officinarum l.) rind. | a green, simple, and effective method for the extraction of sugarcane lipids from sugarcane rind was investigated by response surface methodology. the optimum conditions of technological progress obtained through response surface methodology were as follows: liquid-to-solid ratio 7.94: 1 ml/g, extraction temperature 50°c and extraction time 5.98 h. the practical sugarcane lipids extraction yield was 6.55 ± 0.28%, which was in good consistence with the predicted extraction yield of 6.47%. the res ... | 2014 | 24648272 |
| a comprehensive genetic map of sugarcane that provides enhanced map coverage and integrates high-throughput diversity array technology (dart) markers. | sugarcane genetic mapping has lagged behind other crops due to its complex autopolyploid genome structure. modern sugarcane cultivars have from 110-120 chromosomes and are in general interspecific hybrids between two species with different basic chromosome numbers: saccharum officinarum (2n = 80) with a basic chromosome number of 10 and s. spontaneum (2n = 40-128) with a basic chromosome number of 8. the first maps that were constructed utilised the single dose (sd) markers generated using rflp, ... | 2014 | 24564784 |
| genetic diversity in sugarcane varieties in brazil based on the ward-modified location model clustering strategy. | we evaluated the genetic diversity of 77 clones of sugarcane used in crosses made by the brazilian interuniversity network for the development of the sugar/energy sector (ridesa) breeding program. characterization of the genotypes was carried out at the ratoon stage, based on eight morphological traits and seven agronomic traits. diversity analysis was carried out beginning with the ward-modified location model. the ideal number of groups was three. groups 1, 2, and 3 were composed of 37, 21, an ... | 2014 | 24535897 |
| in vitro selection and characterization of polyethylene glycol (peg) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (saccharum officinarum l.). | a system for in vitro selection of drought tolerant callus lines in sugarcane was developed. high molecular weight peg was used as selective agent. selected callus line grew better than non-selected callus when grown on different concentrations of peg. the activity of antioxidant enzymes like cat, pox, apx and sod were high in selected callus than in non-selected callus. osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at high ... | 2013 | 24431494 |
| phytochemical contents and antioxidant capacities of different parts of two sugarcane (saccharum officinarum l.) cultivars. | antioxidant capacities and phytochemicals such as phenolics, flavonoids, triterpenoids, phytosterols of four parts of two sugarcane cultivars have been characterised. the total triterpenoid content (ttc), total phenolic content (tpc), total flavonoid content (tfc) and total sterol content (tsc) were different among parts and cultivars of sugarcane. the node of both cultivars contained the highest ttc (2096.02 and 1779.66 mg ursolic acid/100g dw for green-rind and red-rind sugarcane, respectively ... | 2014 | 24423556 |
| effects of sugarcane waste-products on cd and zn fractionation and their uptake by sugarcane (saccharum officinarum l.). | the effects of three sugarcane waste-products from an ethanol production plant on the fractionation of cd and zn in high cd and zn contaminated soil and metal accumulation in sugarcane (saccharum officinarum l.) were studied, using the bcr sequential extraction and aqua regia extraction procedures. a pot experiment was performed for 4 months with four treatments: no-amendments (control), boiler ash (3% w/w), filter cake (3% w/w) and a combination of boiler ash and vinasse (1.5% + 1.5%, w/w). the ... | 2014 | 24217524 |
| phenolic profile, antioxidant potential and dna damage protecting activity of sugarcane (saccharum officinarum). | the present study investigated the antioxidant and phenolic composition of sugarcane. the leaves and juices of thirteen varieties of sugarcane were studied for their antioxidant activity and protective effect on dna damage. 2,2-diphenyl-1-picrylhydrazyl radical (dpph) assay was used to determine the radical scavenging activities in leaves and juices. different varieties of sugarcane showed good antioxidant properties, ic50 values ranged from 20.82 to 27.47 μg/ml for leaves and from 63.95 to high ... | 2014 | 24206679 |
| cloning, expression and characterization of sugarcane (saccharum officinarum l.) transketolase. | pentose phosphate pathway (ppp) composed of two functionally-connected phases, the oxidative and non-oxidative phase. both phases catalysed by a series of enzymes. transketolase is one of key enzymes of non-oxidative phase in which transfer two carbon units from fructose-6-phosphate to erythrose-4-phosphate and convert glyceraldehyde-3-phosphate to xylulose-5-phosphate. in plant, erythrose-4-phosphate enters the shikimate pathway which is produces many secondary metabolites such as aromatic amin ... | 2013 | 24132392 |
| involvement of anthocyanins in the resistance to chilling-induced oxidative stress in saccharum officinarum l. leaves. | whether anthocyanins elevate resistance to chilling-induced oxidative stress in saccharum officinarum l. cv badila seedlings is investigated. plants with four fully expanded leaves were exposed to chilling stress (8 °c/4 °c, 11 h photoperiod) for 3 days and then transferred to rewarming condition (25 °c/20 °c, 11 h photoperiod) for another 2 days. at the end of the chilling period, h2o2 and superoxide radical (o2-) levels increased sharply and were near the same in the central (cl) and the final ... | 2013 | 23932150 |
| an-overview on invertase in sugarcane. | saccharum officinarum is one of the most cultivated hybrid varieties among the sugarcane varieties. in sugarcane plant sucrose is the major carbohydrate which can be stored and transported. different physiological and biochemical studies on this crop report that invertase activity and sucrose concentration some how are key limiting step in the process of sucrose accumulation. significant efforts have been made in relation to the sucrose cycle by altering the sucrose phosphate synthetase, sucrose ... | 2013 | 23847400 |
| comparative feedstock analysis in setaria viridis l. as a model for c4 bioenergy grasses and panicoid crop species. | second generation feedstocks for bioethanol will likely include a sizable proportion of perennial c4 grasses, principally in the panicoideae clade. the panicoideae contain agronomically important annual grasses including zea mays l. (maize), sorghum bicolor (l.) moench (sorghum), and saccharum officinarum l. (sugar cane) as well as promising second generation perennial feedstocks including miscanthus×giganteus and panicum virgatum l. (switchgrass). the underlying complexity of these polyploid gr ... | 2013 | 23802002 |
| short-term effects of sugarcane waste products from ethanol production plant as soil amendments on sugarcane growth and metal stabilization. | numerous waste products have been widely studied and used as soil amendments and metal immobilizing agents. waste utilization from ethanol production processes as soil amendments is one of the most promising and sustainable options to help utilize materials effectively, reduce waste disposal, and add value to byproducts. as a consequence, this present work carried out a four-month pot experiment of sugarcane (saccharum officinarum l.) cultivation in cd and zn contaminated soil to determine the e ... | 2013 | 23511210 |
| sugarcane bagasse treated with hydrous ferric oxide as a potential adsorbent for the removal of as(v) from aqueous solutions. | the mechanism of as(v) removal from aqueous solutions by means of hydrated ferric oxide (hfo)-treated sugarcane bagasse (scb-hfo) (saccharum officinarum l.) was investigated. effects of different parameters, such as ph value, initial arsenic concentration, adsorbent dosage, contact time and ionic strength, on the as(v) adsorption were studied. the adsorption capacity of scb-hfo for as(v) was found to be 22.1 mg/g under optimum conditions of ph 4, contact time 3h and temperature 22 °c. initial as ... | 2013 | 23265467 |
| tissue-specific expression and functional role of dehydrins in heat tolerance of sugarcane (saccharum officinarum). | studies on the functional roles of dehydrins (dhns) in heat tolerance of plants are scarce. this study was conducted to immunohistolocalize dhns in leaves of heat-tolerant (cp-4333) and heat-sensitive (hsf-240) sugarcane (saccharum officinarum l.) clones at three phenological stages in order to elucidate their putative roles under heat stress. cp-4333 indicated greater amounts of heat-stable proteins than hsf-240 under heat stress. western blotting revealed the expression of three dhns in cp-433 ... | 2013 | 22886313 |
| halopriming mediated salt and iso-osmotic peg stress tolerance and, gene expression profiling in sugarcane (saccharum officinarum l.). | seed priming is a well known pre-germination strategy that improves seed performance. however, biochemical and molecular mechanisms underlying priming mediated stress tolerance are little understood. here, we report results of the study on growth, physiological characteristics and expression of stress responsive genes in salt primed sugarcane cv. co 86032 plants in response to salt (nacl, 150 mm) or iso-osmotic (-0.7 mpa) polyethylene glycol-peg 8000 (20 % w/v) stress exposure for 15 days. varia ... | 2012 | 22740137 |
| enrichment of genomic dna for polymorphism detection in a non-model highly polyploid crop plant. | large polyploid genomes of non-model species remain challenging targets for dna polymorphism discovery despite the increasing throughput and continued reductions in cost of sequencing with new technologies. for these species especially, there remains a requirement to enrich genomic dna to discover polymorphisms in regions of interest because of large genome size and to provide the sequence depth to enable estimation of copy number. various methods of enriching dna have been utilised, but some re ... | 2012 | 22624722 |
| development of novel chloroplast microsatellite markers for miscanthus species (poaceae). | a set of novel chloroplast microsatellite markers (cpssrs) was developed for the bioenergy crop miscanthus, and their utility in cross-species amplification was evaluated. | 2012 | 22615308 |
| [cloning and expression pattern of a zinc finger protein gene shsap1 in saccharum officinarum]. | in plants, proteins with a20/an1 zinc finger domain are involved in stress responses, named as "stress associated protein" (sap) gene family. based on expressed sequence tag (est) sequences information in badila saccharum officinarum mature related cdna library, we cloned an sap gene from sugarcane full length cdna library, named shsap1 (genbank: accession no. hm991960). to characterize shsap1, we analyzed its genome structure and expression pattern. southern blot analysis showed shsap1 was pres ... | 2011 | 22034815 |
| inference of subgenomic origin of bacs in an interspecific hybrid sugarcane cultivar by overlapping oligonucleotide hybridizations. | sugarcane (saccharum spp.) breeders in the early 20th century made remarkable progress in increasing yield and disease resistance by crossing saccharum spontaneum l., a wild relative, to saccharum officinarum l., a traditional cultivar. modern sugarcane cultivars have approximately 71%-83% of their chromosomes originating from s. officinarum, approximately 10%-21% from s. spontaneum, and approximately 2%-13% recombinant or translocated chromosomes. in the present work, c(0)t-based cloning and se ... | 2011 | 21883018 |
| antioxidant and tyrosinase inhibitory constituents from a desugared sugar cane extract, a byproduct of sugar production. | recycling agricultural resources has become an important issue worldwide promoting the economical value of agricultural production processes. desugared sugar cane extract (dse) from saccharum officinarum is a byproduct obtained during sugar production. two new neolignan glucosides, saccharnan a (1) and saccharnan b (2), together with 10 known phenolics (3-12) were isolated from dse, and their structures were elucidated on the basis of nmr spectral analysis. compounds 3, 4, 8, and 9 showed good a ... | 2011 | 21770441 |
| global climate niche estimates for bioenergy crops and invasive species of agronomic origin: potential problems and opportunities. | the global push towards a more biomass-based energy sector is ramping up efforts to adopt regionally appropriate high-yielding crops. as potential bioenergy crops are being moved around the world an assessment of the climatic suitability would be a prudent first step in identifying suitable areas of productivity and risk. additionally, this assessment also provides a necessary step in evaluating the invasive potential of bioenergy crops, which present a possible negative externality to the bioec ... | 2011 | 21408056 |
| phosphorus speciation in sequentially extracted agro-industrial by-products: evidence from x-ray absorption near edge structure spectroscopy. | the phosphorus (p) in agro-industrial by-products--a potential source of freshwater eutrophication but also a valuable fertilizer--needs to be speciated to evaluate its fate in the environment. we investigated to what extent x-ray absorption near edge structure (xanes) spectroscopy at the p k- and l2.3-edges reflected differences in sequentially extracted filter cakes from sugarcane (saccharum officinarum l.) (fic) and niger seed (guizotia abyssinica cass.; nic) processing industry in ethiopia. ... | 2013 | 21284316 |
| c4 plants as biofuel feedstocks: optimising biomass production and feedstock quality from a lignocellulosic perspective. | the main feedstocks for bioethanol are sugarcane (saccharum officinarum) and maize (zea mays), both of which are c(4) grasses, highly efficient at converting solar energy into chemical energy, and both are food crops. as the systems for lignocellulosic bioethanol production become more efficient and cost effective, plant biomass from any source may be used as a feedstock for bioethanol production. thus, a move away from using food plants to make fuel is possible, and sources of biomass such as w ... | 2011 | 21205189 |
| modeling of phosphorus loads in sugarcane in a low-relief landscape using ontology-based simulation. | water flow and p dynamics in a low-relief landscape manipulated by extensive canal and ditch drainage systems were modeled utilizing an ontology-based simulation model. in the model, soil water flux and processes between three soil inorganic p pools (labile, active, and stable) and organic p are represented as database objects. and user-defined relationships among objects are used to automatically generate computer code (java) for running the simulation of discharge and p loads. our objectives w ... | 2013 | 21043280 |
| phylogenetic relationships among saccharum clones in pakistan revealed by rapd markers. | forty sugarcane genotypes (clones), including elite lines, commercial cultivars of saccharum officinarum and s. barberi clones, were fingerprinted with 30 rapd markers, using a pcr-based marker assay. the genetic distance for rapd data was determined according to nei, and relationships between accessions were graphed in a dendrogram. genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. the lowest genetic distance was found between genotypes us-406 a ... | 2010 | 20799164 |
| setaria viridis: a model for c4 photosynthesis. | c(4) photosynthesis drives productivity in several major food crops and bioenergy grasses, including maize (zea mays), sugarcane (saccharum officinarum), sorghum (sorghum bicolor), miscanthus x giganteus, and switchgrass (panicum virgatum). gains in productivity associated with c(4) photosynthesis include improved water and nitrogen use efficiencies. thus, engineering c(4) traits into c(3) crops is an attractive target for crop improvement. however, the lack of a small, rapid cycling genetic mod ... | 2010 | 20693355 |
| gish characterization of erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids. | within erianthus, a genus close to saccharum, the species e. arundinaceus has the potential to contribute valuable traits to sugarcane, including adaptation to biotic and abiotic stresses and ratooning ability. sugarcane breeders have tried for a long time to use erianthus species in their breeding programs but until recently were constrained by a lack of fertile saccharum x erianthus hybrids. we report here for the first time the chromosome composition of fertile saccharum officinarum x e. arun ... | 2010 | 20616864 |
| genetic studies of "noble cane" for identification and exploitation of genetic markers. | forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of saccharum officinarum and clones of s. barberi were fingerprinted with 50 ssr markers using a pcr-based marker assay. nei's genetic distances for ssr data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. the shortest genetic distance of 0.60 was seen betw ... | 2010 | 20533196 |
| dna profiling of sugarcane genotypes using randomly amplified polymorphic dna. | dna profiles of 40 sugarcane genotypes were constructed with 30 rapd markers. sugarcane genotypes of both saccharum officinarum and s. barberi were included in this study. multiple alleles were detected from each rapd; there was a high level of polymorphism. on average, 7.93 alleles were produced per primer, giving a total of 238 alleles. the genetic distances between these genotypes were assessed with the popgene dna sequence analysis software. a dendrogram was constructed from these data; cult ... | 2010 | 20391332 |
| adsorption behavior of heavy metals onto chemically modified sugarcane bagasse. | a new process for the xanthation of sugarcane (saccharum officinarum) bagasse was investigated for the separation of cadmium, lead, nickel, zinc and copper from their aqueous solutions. adsorption capacity of the charred xanthated sugarcane bagasse (cxsb) was found to be significantly more than the several biosorbents reported in the literatures. the modified material was characterized by ftir and elemental analysis. the kinetics of sorption of the tested metals was fast, reaching equilibrium wi ... | 2010 | 20006923 |
| genetic diversity of saccharum officinarum accessions in pakistan as revealed by random amplified polymorphic dna. | genetic diversity of 20 sugarcane accessions in pakistan was studied using 21 random amplified polymorphic dna markers. the mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. this probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage of these varieties. among the varieties, none was found to be totally distinct and divergent from the others ... | 2009 | 19937582 |
| impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane. | a cephalosporin antibiotic, cefotaxime (omnatax™) promoted somatic embryogenesis and subsequent shoot regeneration in vitro from spindle in sugarcane irrespective of the genotypes as (coj 83, coj 88 and coj 64) culturered on ms medium with 2,4-d (2.5 mgl(-1)) and kinetin (0.5 mgl(-1)). seven different concentrations of cefotaxime (100, 200, 300, 400, 500, 600 and 700 mgl(-1)) were tested to find the optimal concentration of cefotaxime for somatic embryogenesis from callus cultures. among the thr ... | 2009 | 23572935 |
| long-term water quality trends after implementing best management practices in south florida. | a mandatory best management practices (bmp) program was implemented in the everglades agricultural area (eaa) farms basin-wide in 1995 as required by the everglades forever act to reduce p loads in drainage water reaching the everglades ecosystem. all farms in the eaa basin implement similar bmps, and basin wide p load reductions have exceeded the 25% reduction required by law; however, differences remain in water quality between subbasins. our objective was to determine long-term trends in p lo ... | 2013 | 19549945 |
| enhancing plant uptake of polychlorinated biphenyls and cadmium using tea saponin. | the potential of natural surfactant tea saponin to enhance uptake of polychlorinated biphenyls (pcbs) and cadmium (cd) by zea mays l. and saccharum officinarum l. was investigated. with addition of tea saponin at 0.01% in solution culture, the concentrations of pcb 14, pcb 18, pcb 77 and pcb 156 in root of corn seedling were 2.72, 2.68, 1.94 and 2.40 times as those of treatments without adding any surfactant, respectively. application of tea saponin to the soil significantly elevated pcb 5 accum ... | 2009 | 19477119 |
| targeted single nucleotide polymorphism (snp) discovery in a highly polyploid plant species using 454 sequencing. | discovering single nucleotide polymorphisms (snps) in specific genes in a heterozygous polyploid plant species, such as sugarcane, is challenging because of the presence of a large number of homologues. to discover snps for mapping genes of interest, 454 sequencing of 307 polymerase chain reaction (pcr) amplicons (> 59 kb of sequence) was undertaken. one region of a four-gasket sequencing run, on a 454 genome sequencer flx, was used for pooled pcr products amplified from each parent of a quantit ... | 2009 | 19386042 |
| stem juice production of the c4 sugarcane (saccharum officinarum) is enhanced by growth at double-ambient co2 and high temperature. | two cultivars of sugarcane (saccharum officinarum cv. cp73-1547 and cp88-1508) were grown for 3 months in paired-companion, temperature-gradient, sunlit greenhouses under daytime [co2] of 360 (ambient) and 720 (double ambient) micromol mol(-1) and at temperatures of 1.5 degrees c (near ambient) and 6.0 degrees c higher than outside ambient temperature. leaf area and biomass, stem biomass and juice and co2 exchange rate (cer) and activities of ribulose bisphosphate carboxylase-oxygenase (rubisco) ... | 2009 | 19217687 |
| nitrogen fixation and translocation in young sugarcane (saccharum officinarum l.) plants associated with endophytic nitrogen-fixing bacteria. | the tracer (15)n(2) was used to investigate sites of n(2) fixation and the possible translocation of the fixed n. young sugarcane plants (saccharum officinarum l.) from a stem cutting were exposed to (15)n(2)-labeled air in a 500 ml plastic cylinder. plants fed (15)n(2) for 7 days were grown in normal air for a further chase period. after 21 days, about half of the n originating in the stem cutting had been transported to the shoot and roots, suggesting that the cutting played a role in supplyin ... | 2009 | 21566377 |
| supply and demand: sink regulation of sugar accumulation in sugarcane. | sugarcane (saccharum spp. hybrids) accumulates sucrose to high concentrations and, as a result, has been the focus of extensive research into the biochemistry and physiology of sucrose accumulation. despite this, the relationship between source leaf photosynthetic activity and sucrose accumulation in the culm sink is not well understood. the observations that photosynthetic activity declines during culm maturation in commercial cultivars and that high-sucrose-accumulating noble ancestral genotyp ... | 2009 | 19050062 |
| nonpoint source of nutrients and herbicides associated with sugarcane production and its impact on louisiana coastal water quality. | a watershed analysis of nonpoint-source pollution associated with sugarcane (saccharum officinarum l.) production was conducted. runoff water samples following major rainfall events from two representative sugarcane fields (sc1 and sc2) were collected and analyzed. the impact of runoff on two receiving water bodies, st. james canal (sjc) and bayou chevreuil (bc) in a drainage basin (baratarian basin), was studied. results show that runoff flow/rainfall ratios at the sc1 were significantly higher ... | 2012 | 18948481 |
| genetic control of yield related stalk traits in sugarcane. | a major focus of sugarcane variety improvement programs is to increase sugar yield, which can be accomplished by either increasing the sugar content of the cane or by increasing cane yield, as the correlation between these traits is low. we used a cross between an australian sugarcane variety q165, and a saccharum officinarum accession, ij76-514, to dissect the inheritance of yield-related traits in the complex polyploid sugarcane. a population of 227 individuals was grown in a replicated field ... | 2008 | 18719878 |
| verification test of sensory analyses of comb and strained honeys produced as pure and feeding intensively with sucrose (saccharum officinarum l.) syrup. | the aims of the study were to discriminate comb and strained honeys produced by the standard beekeeping method (control), shaking method (pure blossom honey), and feeding intensively (100kg/colony) with sucrose (adulterated honey) syrup by using sensory analysis and to develop a method to be used in identification of unknown or suspicion honey samples. in the study, twenty trained panelists assessed honey samples in relation to their properties including taste, odor, color, aroma, viscosity, dis ... | 2008 | 26050005 |
| growth at elevated co(2) delays the adverse effects of drought stress on leaf photosynthesis of the c(4) sugarcane. | sugarcane (saccharum officinarum l. cv. cp72-2086) was grown in sunlit greenhouses at daytime [co(2)] of 360 (ambient) and 720 (elevated)mumolmol(-1). drought stress was imposed for 13d when plants were 4 months old, and various photosynthetic parameters and levels of nonstructural carbohydrates were determined for uppermost fully expanded leaves of well-watered (control) and drought stress plants. control plants at elevated [co(2)] were 34% and 25% lower in leaf stomatal conductance (g(s)) and ... | 2009 | 18462832 |
| genetic analysis for brix weight per stool and its component traits in sugarcane (saccharum officinarum). | brix weight per stool (bw) of sugarcane is a complex trait, which is the final product of a combination of many components. diallel cross experiments were conducted during a period of two years for bw and its five component traits, including stalk diameter (sd), stalk length (sl), stalk number (sn), stalk weight (sw), and brix scale (bs) of sugarcane. phenotypic data of all the six traits were analyzed by mixed linear model and their phenotype variances were portioned into additive (a), dominanc ... | 2007 | 18257118 |
| construction of a genetic linkage map for saccharum officinarum incorporating both simplex and duplex markers to increase genome coverage. | saccharum officinarum l. is an octoploid with 80 chromosomes and a basic chromosome number of x = 10. it has high stem sucrose and contributes 80% of the chromosomes to the interspecific sugarcane cultivars that are grown commercially for sucrose. a genetic linkage map was developed for s. officinarum (clone ij76-514) using a segregating population generated from a cross between q165 (a commercial sugarcane cultivar) and ij76-514. in total, 40 aflp and 72 ssr primer pairs were screened across th ... | 2007 | 17893734 |
| domestication to crop improvement: genetic resources for sorghum and saccharum (andropogoneae). | both sorghum (sorghum bicolor) and sugarcane (saccharum officinarum) are members of the andropogoneae tribe in the poaceae and are each other's closest relatives amongst cultivated plants. both are relatively recent domesticates and comparatively little of the genetic potential of these taxa and their wild relatives has been captured by breeding programmes to date. this review assesses the genetic gains made by plant breeders since domestication and the progress in the characterization of geneti ... | 2007 | 17766842 |
| kinetic model of sucrose accumulation in maturing sugarcane culm tissue. | biochemically, it is not completely understood why or how commercial varieties of sugarcane (saccharum officinarum) are able to accumulate sucrose in high concentrations. such concentrations are obtained despite the presence of sucrose synthesis/breakdown cycles (futile cycling) in the culm of the storage parenchyma. given the complexity of the process, kinetic modelling may help to elucidate the factors governing sucrose accumulation or direct the design of experimental optimisation strategies. ... | 2007 | 17555779 |
| pcdd and pcdf emissions from simulated sugarcane field burning. | the emissions from simulated sugarcane (saccharum officinarum) field burns were sampled and analyzed for polychlorinated dibenzodioxins and dibenzofurans (pcdds and pcdfs). sugarcane leaves from hawaii and florida were burned in a manner simulating the natural physical dimensions and biomass density found during the practice of preharvest field burning. eight composite burn tests consisting of 3-33 kg of biomass were conducted, some with replicate samplers. emission factor calculations using sam ... | 2006 | 17120546 |
| physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane (saccharum officinarum) sprouts. | global increase in ambient temperature is a critical factor for plant growth. in order to study the changes in growth over short intervals, various primary and secondary metabolites, and their relationships with thermotolerance, 1-month-old sugarcane (saccharum officinarum) sprouts were grown under control conditions (28 degrees c) or under heat-stress conditions (40 degrees c), and measurements were made at six 12-h intervals. heat stress greatly reduced dry matter and leaf area of sprouts init ... | 2007 | 17024517 |
| on-line identification of further flavone c- and o-glycosides from sugarcane (saccharum officinarum l., gramineae) by hplc-uv-ms. | hplc-uv-ms (apci-ms/ms and cid/ms) was utilised for the identification of eight additional flavone glycosides from sugarcane (saccharum officinarum l., gramineae) extracts of bagasse, leaves and juice ('garapa'). relevant information about substitution patterns was obtained through uv detection using post-column addition of shift reagents, while tandem ms provided structural information confirming the proposed structures of the c-glycosides vitexin, orientin, luteolin-8-c-(rhamnosylglucoside), 4 ... | 2012 | 17019935 |
| genetic segregation of microsatellite markers in saccharum officinarum and s. spontaneum. | genetic mapping techniques can be used to study the interaction between two different genomes after hybridization. this study investigated a saccharum officinarum (green german or gg, 2n approximately 11x approximately 110) x s. spontaneum (ind 81-146 or ind, 2n approximately 7x approximately 56) interspecific cross. segregation of 193 microsatellite (ssr) loci was evaluated in the f(1) progeny of 169 full-sibs of the cross. following the two-way pseudo-testcross strategy and 'cross pollination' ... | 2006 | 16912699 |
| plant growth inhibitors isolated from sugarcane (saccharum officinarum) straw. | several compounds related with plant defense and pharmacological activities have been isolated from sugarcane. straw phytotoxins and their possible mechanisms of growth inhibition are largely unknown. a bioassay-guided fractionation of the phytotoxic constituents leachated from a sugarcane straw led to the isolation of trans-ferulic (trans-fa), cis-ferulic (cis-fa), vanillic (va) and syringic (sa) acids. the straw leachates and their identified constituents significantly inhibited root growth of ... | 2006 | 16777531 |
| quantitative trait loci identified for sugar related traits in a sugarcane (saccharum spp.) cultivar x saccharum officinarum population. | the identification of markers linked to quantitative trait loci (qtls) for increased sugar accumulation could improve the effectiveness of current breeding strategies in sugarcane. progeny from a cross between a high sucrose producing cultivar, (denotes australian plant breeding rights), and a saccharum officinarum clone, ij76-514 were grown in two field experiments in different years, and evaluated in the early and mid-season phases of crop maturity, to identify robust qtls in affecting sucrose ... | 2006 | 16508765 |
| effect of d-003, a mixture of very-long-chain aliphatic acids purified from sugarcane wax, on cerebral ischemia in mongolian gerbils. | d-003 is a mixture of very-high-molecular-weight aliphatic acids purified from sugar cane wax (saccharum officinarum), which inhibits platelet aggregation and lipid peroxidation. the objective of the present study was to evaluate the effect of d-003 on cerebral ischemia induced by ischemia-reperfusion (i-r) in mongolian gerbils. two experimental series were conducted. the first series investigated the effects of d-003 on cerebral edema, neurological symptoms, and mortality in mongolian gerbils w ... | 2005 | 16379559 |
| assessment of herbicide leaching risk in two tropical soils of reunion island (france). | application of organic chemicals to a newly irrigated sugarcane (saccharum officinarum l.) area located in the semiarid western part of reunion island has prompted local regulatory agencies to determine their potential to contaminate ground water resources. for that purpose, simple indices known as the ground water ubiquity score (gustafson index, gus), the retardation factor (rf), the attenuation factor (af), and the log-transformed attenuation factor (aft) were employed to assess the potential ... | 2006 | 15758106 |
| assessment of variables controlling nitrate dynamics in groundwater: is it a threat to surface aquatic ecosystems? | the impact of fertilised cropping on nitrate-n dynamics in groundwater (gw) was assessed in a catchment from piezometers installed: (i) to different depths, (ii) in different soil types, (iii) on different positions on landscape, and (iv) compared with the australian and new zealand environmental and conservation council guideline values provided for different aquatic ecosystems. the gw and no(3)-n concentration dynamics were monitored in 39 piezometer wells, installed to 5-90 m depth, under fer ... | 2005 | 15757708 |
| unraveling the genome structure of polyploids using fish and gish; examples of sugarcane and banana. | we review here the progress that has been achieved using molecular cytogenetics to analyze the genome structure of sugarcane (saccharum spp) and banana (musa spp), two crops that are polyploid, of interspecific origin and with chromosomes not distinguishable by their gross morphology. in saccharum, molecular cytogenetics enabled us to determine the basic chromosome number of two species, saccharum officinarum and s. spontaneum, involved in the origin of modern cultivars, to quantify the proporti ... | 2005 | 15753555 |
| a combination of aflp and ssr markers provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar. | sugarcane varieties are complex polyploids carrying in excess of 100 chromosomes and are derived from interspecific hybridisation between the domesticated saccharum officinarum and the wild relative s. spontaneum. a map was constructed in denotes variety covered by australian plant breeding rights., an australian cultivar, from a segregating f1 population, using 40 amplified fragment length polymorphism (aflp) primer combinations, five randomly amplified dna fingerprints (raf) primers and 72 sim ... | 2005 | 15700149 |
| structural features and transcript-editing analysis of sugarcane (saccharum officinarum l.) chloroplast genome. | the complete nucleotide sequence of the chloroplast genome of sugarcane (saccharum officinarum) was determined. it consists of 141,182 base-pairs (bp), containing a pair of inverted repeat regions (ir(a), ir(b)) of 22,794 bp each. the ir(a) and ir(b) sequences separate a small single copy region (12,546 bp) and a large single copy (83,048 bp) region. the gene content and relative arrangement of the 116 identified genes (82 peptide-encoding genes, four ribosomal rna genes, 30 trna genes), with th ... | 2004 | 15526204 |
| differential chromosome pairing affinities at meiosis in polyploid sugarcane revealed by molecular markers. | chromosome pairing at meiosis is an essential feature in cell biology, which determines trait inheritance and species evolution. complex polyploids may display diverse pairing affinities and offer favorable situations for studying meiosis. the genus saccharum encompasses diverse forms of polyploids with predominantly bivalent pairing. we have focused on a modern cultivar of sugarcane, r570, and taken advantage of a particular single copy probe (bnl 12.06) revealing 11 alleles by restriction frag ... | 2004 | 15292909 |
| genetic diversity and relationships in native hawaiian saccharum officinarum sugarcane. | commercial sugarcane hybrid cultivars currently in production are high-yielding, disease-resistant, millable canes and are the result of years of breeding work. in hawaii, these commercial hybrids are quite distinct from many saccharum officinarum canes still in existence that were brought to the islands and cultivated by the native polynesians. the actual genetic relationships among the native canes and the extent to which they contributed to the commercial hybrid germplasm has been the subject ... | 2008 | 15247312 |
| genome remodelling in three modern s. officinarumxs. spontaneum sugarcane cultivars. | this study provides evidence that nuclear and chromosome remodelling has taken place in sugarcane, a vegetative crop with a complex genome derived from interspecific hybridizations between saccharum officinarum and s. spontaneum. detailed knowledge on the chromosomal compositions of the three clones analysed was acquired. (1) all hybrid cultivars were found to be aneuploid, affecting both parental genomes (having chromosomes in addition to full genomes), with chromosome numbers from 2n=102-106 i ... | 2004 | 14990623 |
| response of sugarcane to treated wastewater of oil refinery. | the crop of sugarcane (saccharum officinarum) was grown at the agricultural farm of the mathura oil refinery in a simple randomized block design. the experimental plots were irrigated with ground water (gw) or treated wastewater (tw) as and when required the plants gave better response to the latter than the former. the quantity of the required nutrients was comparatively more in tw than gw. the soil receiving wastewater did not show any significant change in its physico-chemical characteristics ... | 2003 | 12974454 |
| new phenolic compounds from kokuto, non-centrifuged cane sugar. | five new phenolic compounds, 4-(beta-d-glucopyranosyloxy)-3,5-dimethoxyphenyl-propanone (8), 3-[5-[(threo) 2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxybenzofuranyl]-propanoic acid (12), 2-[4-(3-hydroxy-1-propenyl)-2,6-dimethoxyphenoxy]-3-hydroxy-3-(4-hydroxy-3,5-dimethoxyphenyl)propyl-beta-d-glucopyranoside (13), 4-[(erythro) 2,3-dihydro-3(hydroxymethyl)-5-(3-hydropropyl)-7-methoxy-2-benzofuranyl]-2,6-dimethoxyphenyl-beta-d-glucopyranoside (14), 9-o-beta-d-xylopyranoside o ... | 2003 | 12729002 |
| comparative analysis of qtls affecting plant height and flowering among closely-related diploid and polyploid genomes. | quantitative trait loci (qtls) affecting plant height and flowering were studied in the two saccharum species from which modern sugarcane cultivars are derived. two segregating populations derived from interspecific crosses between saccharum officinarum and saccharum spontaneum were genotyped with 735 dna markers. among the 65 significant associations found between these two traits and dna markers, 35 of the loci were linked to sugarcane genetic maps and 30 were unlinked dna markers. twenty-one ... | 2002 | 12416611 |
| behaviour of ribosomal genes and nucleolar domains during activation in sugarcane (saccharum officinarum l.) root primordia: from the unsoaked quiescent state to the steady state of proliferation. | changes in the organisation of ribosomal genes and nucleolar protein components were analysed in sugarcane (saccharum officinarum l. cv cristalina) from the time the quiescent primordia of the radical bands of nodes were stimulated to proliferate by water imbibition, until the meristematic population reached the steady state of proliferation in the growing roots. the kinetics of proliferation was evaluated by flow cytometry, and by the mitotic indexes, in roots of different lengths. all the quie ... | 2002 | 12152792 |
| coiled bodies in nuclei from plant cells evolving from dormancy to proliferation. | to characterise the coiled bodies in meristematic nuclei of saccharum officinarum, immunofluorescence labelling with antibodies against components of the splicing (u2b'' and sm core protein b) and pre-rrna processing (fibrillarin) complexes was used in cells from the dormant root primordia and from roots at different times after activation to the steady state of proliferation. the number, size and distribution of coiled bodies varied in the meristematic tissue depending on cell activity. while g ... | 2002 | 12068973 |
| growth of am fungi on in vitro root organ culture of sorghum vulgare and saccharum officinarum. | spores of gl mosseae and gig gigantea germinated on minimal medium produced extraradical mycelium. gl. mosseae infected roots of s. officinarum in in vitro condition were inoculated in m medium with in vitro cultured roots of sorghum vulgare (test roots). from the infected root of s. officinarum, the mycelium developed and it infected the test roots. the roots developed new mycelia and further the mycelia produced a few hyaline spores. in ms medium combined with soil extract, root exudate, thiam ... | 2001 | 12018527 |
| effect of maturity on chlorophyll, tannin, color, and polyphenol oxidase (ppo) activity of sugarcane juice (saccharum officinarum var. yellow cane). | a study was conducted to determine the effect of sugarcane maturation on the contents of chlorophyll, tannin, and polyphenol oxidase (ppo) activity and on color change of sugarcane juice. the maturation period of the cane studied was between 3 and 10 months after planting. different parts of the cane, namely, the top, middle, and bottom portions, were analyzed. results obtained indicated that there were significant (p < 0.01) decreases in total chlorophyll a and b and tannin contents during matu ... | 2002 | 11879045 |
| influence of ph and industrial activity on total zinc concentrations in agricultural soils, sewage sludges, and beach sands: relationship with plant (saccharum officinarum) availability. | | 2002 | 11815792 |
| sugar cane (saccharum officinarum l) burning and asthma in southeast louisiana, usa. | | 2002 | 11815785 |
| expression of different abscisic acid-responsive genes during somatic embryogenesis in sugarcane (saccharum officinarum). | we have examined the expression of four genes during somatic embryogenesis in two cultivars of sugarcane, saccharum officinarum, one drought resistant (ja-605) and the other one sensitive (c-8751), as well as in embryogenic and nonembryogenic tissues treated with abscisic acid (aba) or after drought stress. three of the cdnas probed, from plasmids pma1049, pma2005 and cm1, were lea genes and the other one, from a barley hemoglobin gene, was induced in other species under stress conditions. only ... | 2001 | 11732312 |
| analysis of sucrose accumulation in the sugar cane culm on the basis of in vitro kinetic data. | sucrose accumulation in developing sugar cane (saccharum officinarum) is accompanied by a continuous synthesis and cleavage of sucrose in the storage tissues. despite numerous studies, the factors affecting sucrose accumulation are still poorly understood, and no consistent pattern has emerged which pinpoints certain enzyme activities as important controlling steps. here, we develop an approach based on pathway analysis and kinetic modelling to assess the biochemical control of sucrose accumulat ... | 2001 | 11513743 |
| dormancy and proliferation in saccharum officinarumxs. spontaneum hybrids which differ in the number of the introgressed s. spontaneum chromosomes. | proliferating cells remain transiently blocked at different cycle compartments until specific stressors are removed or until the cells become adapted to their presence. this paper investigates the efficiency of cycle blocks in three sugarcane hybrids with the full noble cane (saccharum officinarum) genome (2n=8x=80) but differing in the number of introgressed s. spontaneum (2n=8x=64) chromosomes. the my5514, b42231 and c236-51 cultivars possess 20, 30 and 40 additional s. spontaneum chromosomes, ... | 2001 | 11432938 |
| magnetic resonance imaging (mri) of saccharum officinarum l. (sugarcane) during its growth for sucrose content. | magnetic resonance imaging (mri) was employed to monitor changes in image intensities in stems of sugarcane which reflect on the increase in sucrose concentration. contrast in images originates in the increase of sucrose concentration in the aqueous phase of the predominant parenchyma cells and physiological changes. in matured stems mixed mr intensity patterns were observed in transverse planes. we associate this due to the reflection of vascular bundles in ground parenchyma cells which constit ... | 2000 | 11324162 |
| policosanol modulates hmg-coa reductase activity in cultured fibroblasts. | cholesterol biosynthesis is strictly controlled by 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase. | 2013 | 11282173 |
| molecular contribution to selection of intergeneric hybrids between sugarcane and the wild species erianthus arundinaceus. | erianthus arundinaceus has great potential as a germplasm source for better ratoonability, vigour, tolerance to environmental stresses, and disease resistance in sugarcane. many unsuccessful attempts have been made to introduce these characters into modern sugarcane cultivars. we report on significant progress made since molecular tools were implemented. sequence-tagged pcr, revealing size variation in the 5s rdna cluster, was performed on intact leaf tissue to identify genuine hybrids six weeks ... | 2000 | 11195335 |
| metabolite monitoring in plants with double-quantum filtered chemical shift imaging. | 1h spectroscopic imaging in combination with double-quantum filtering with magnetic field gradients is used for in vivo applications in humans and animals. because of its high selectivity and strong reduction of water signal it is also a useful tool for monitoring the distribution of specific metabolites in plants. the development and application of a double-quantum selective spectroscopic imaging sequence for detecting the sucrose distribution in the stem of sugar cane (saccharum officinarum l. ... | 2000 | 11141184 |
| distribution of plant-parasitic nematodes on sugarcane in louisiana and efficacy of nematicides. | a survey conducted from may 1995 through august 1998 revealed diverse nematode communities in louisiana sugarcane fields. high populations of mesocriconema, paratrichodorus, pratylenchus, and tylenchorhynchus were widespread in nine sugarcane production parishes. comparisons of plant cane and ratoon sugarcane crops indicated that nematode community levels increase significantly in successive ratoon crops. nematicide trials evaluated the efficacy of aldicarb, ethoprop, and phorate against indigen ... | 2000 | 19271000 |
| detailed alignment of saccharum and sorghum chromosomes: comparative organization of closely related diploid and polyploid genomes. | the complex polyploid genomes of three saccharum species have been aligned with the compact diploid genome of sorghum (2n = 2x = 20). a set of 428 dna probes from different poaceae (grasses) detected 2460 loci in f1 progeny of the crosses saccharum officinarum green german x s. spontaneum ind 81-146, and s. spontaneum pin 84-1 x s. officinarum muntok java. thirty-one dna probes detected 226 loci in s. officinarum la purple x s. robustum molokai 5829. genetic maps of the six saccharum genotypes, ... | 1998 | 9832541 |
| comparative mapping of andropogoneae: saccharum l. (sugarcane) and its relation to sorghum and maize. | comparative genetic maps of papuan saccharum officinarum l. (2n = 80) and s. robustum (2n = 80) were constructed by using single-dose dna markers (sdms). sdm-framework maps of s. officinarum and s. robustum were compared with genetic maps of sorghum and maize by way of anchor restriction fragment length polymorphism probes. the resulting comparisons showed striking colinearity between the sorghum and saccharum genomes. there were no differences in marker order between s. officinarum and sorghum. ... | 1997 | 9405600 |
| using genomic slot blot hybridization to assess intergeneric saccharum x erianthus hybrids (andropogoneae - saccharinae). | the use of genomic slot blot hybridization enabled the differentiation of hybrids from selfs in saccharum x erianthus intergeneric crosses in which saccharum was used as the female parent. based on the genomic in situ hybridization technique, slot blots of dna from the parents and the progeny were blocked with the saccharum parent dna and hybridized with the labelled male erianthus genomic dna. this technique allowed a rapid screening for hybrids and was sensitive enough to detect a 1/20 dilutio ... | 1997 | 18464838 |
| rflp mapping in cultivated sugarcane (saccharum spp.): genome organization in a highly polyploid and aneuploid interspecific hybrid. | sugarcane cultivars are polyploid, aneuploid, interspecific hybrids between the domesticated species saccharum officinarum and the wild relative s. spontaneum. cultivar chromosome numbers range from 100 to 130 with approximately 10% contributed by s. spontaneum. we have undertaken a mapping study on the progeny of a selfed cultivar, r570, to analyze this complex genome structure. a set of 128 restriction fragment length polymorphism probes and one isozyme was used. four hundred and eight markers ... | 1996 | 8849904 |
| detection of rdna sites in sugarcane by fish. | hybridization sites of an rdna probe coding for the 5.8s, 18s and 26s genes were detected on the chromosomes of sugarcane and a related genus, erianthus, using fluorescence in situ hybridization. one unpaired and five paired hybridization sites were detected in a saccharum spp. hybrid. a first introgression hybrid (i1) between saccharum officinarum and saccharum spontaneum had seven pairs of hybridization sites. a clone of erianthus arundinaceus showed six hybridization sites in somatic tissue. | 1995 | 8528591 |
| identification and characterisation of sugarcane intergeneric hybrids, saccharum officinarum x erianthus arundinaceus, with molecular markers and dna in situ hybridisation. | molecular markers were used to characterise sugarcane intergeneric hybrids between s. officinarum and e. arundinaceus. very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged pcr. two enzyme systems (got and mdh b) and pcr amplification revealing spacer-size variation in the 5s-rdna cluster were found most convenient. specific characterisation of the two genomic components was possible using rflp and in situ hybridi ... | 1995 | 24169780 |
| production of transgenic sugarcane (saccharum officinarum l.) plants by intact cell electroporation. | we describe an efficient procedure for genetic transformation of commercial sugarcane varieties poj 2878 and ja 60-5. the transformation protocol is based on electroporation of a plasmid conferring gus activity into cell clusters isolated from embryogenic calli. six to eight weeks after electroporation, ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and southern hybridization analysis. electroporation of intact ... | 1995 | 24186765 |
| chromosome assortment in saccharum. | recent work has revealed random chromosome pairing and assortment in saccharum spontaneum l., the most widely distributed, and morphologically and cytologically variable of the species of saccharum. this conclusion was based on the analysis of a segregating population from across between s. spontaneum 'ses 208' and a spontaneously-doubled haploid of itself, derived from anther culture. to determine whether polysomic inheritance is common in saccharum and whether it is observed in a typical bipar ... | 1994 | 24178110 |
| protein variation accompanies leaf dedifferentiation in sugarcane (sacchamm officinarum) and is influenced by genotype. | tissue culture lines (morphogenic and nonmorphogenic) were established in five genotypes of saccharum officinarum l. changes in protein expression after dedifferentiation of leaf tissue into callus were investigated by two-dimensional gel electrophoresis of cellular proteins. the findings demonstrated that protein expression was regulated both quantitatively and qualitatively in all five genotypes of sugarcane. sixty-three dedifferention-proteins were identified, of which thirty-three were commo ... | 1994 | 24193522 |
| enhanced gus gene expression in cereal/grass cell suspensions and immature embryos using the maize uhiquitin-based plasmid pahc25. | transient gus (β-glucuronidase) expression was visualized in cell suspensions of triticum aestivum, zea mays, pennisetum glaucum, saccharum officinarum, pennisetum purpureum and panicum maximum after microprojectile bombardment with pbargus and pahc25 plasmid dnas. pbargus contains the gus (uida) gene coding region driven by the adh1 promoter and the adh1 intron 1, as well as the bar gene coding region driven by the camv 35s promoter and the adh1 intron 1. pahc25 contains the gus and bar gene co ... | 1993 | 24196107 |
| bacterial flora in the rhizosphere of sugarcane plant, saccharum officinarum. | streptomycin-resistant bacteria were recovered from the root-free soils under sugarcane plants. nitrosomonas species and azotobacter chrococcum were also present in greater numbers in rhizosphere soils as compared to root-free soils up to 30 cm deep. | 1991 | 24425034 |
| protein polymorphism in sugarcane revealed by two-dimensional gel analysis. | in order to identify molecular markers for the analysis of the sugarcane genome, proteins extracted from apical segments of shoot tissues were resolved by a combination of equilibrium (ief) and nonequilibrium (nephge) two-dimensional polyacrylamide gel electrophoresis. a number of taxa of the "saccharum complex" group (saccharum species and the related genera of andropogoneae) with presumed contributions to the sugarcane genome were surveyed. protein profiles were compared to a reference map con ... | 1990 | 24226346 |
| characterization of the heat shock response in cultured sugarcane cells : i. physiology of the heat shock response and heat shock protein synthesis. | effect of heat shock on the growth of cultured sugarcane cells (saccharum officinarum l.) was measured. heat shock (hs) treatment at 36 to 38 degrees c (2 hours) induced the development of maximum thermotolerance to otherwise nonpermissive heat stress at 54 degrees c (7 minutes). optimum thermotolerance was observed 8 hours after heat shock. development of thermotolerance was initiated by treatments as short as 30 minutes at 36 degrees c. temperatures below 36 degrees c or above 40 degrees c fai ... | 1989 | 16666866 |
| analysis of mitochondrial dna from somatic hybrid cell lines of saccharum officinarum (sugarcane) and pennisetum americanum (pearl millet). | mitochondrial dna (mtdna) from cell suspension cultures of two intergeneric somatic hybrids of pennisetum americanum (pearl millet) + saccharum officinarum (sugarcane) was examined by restriction endonuclease digestion and hybridization with sorghum mtdna cosmids. the mtdna of one somatic hybrid was indistinguishable from that of pearl millet, while the second exhibited a combination of parental mtdnas, suggesting mitochondrial fusion. several novel, possibly recombinant, mtdna restriction fragm ... | 1987 | 24301313 |
| somatic hybridization in the gramineae: saccharum officinarum l. (sugarcane) and pennisetum americanum (l.) k. schum. (pearl millet). | somatic hybrid cell lines with embryogenic capacity were obtained by fusion of protoplasts isolated from an embryogenic cell line of sugarcane with inactivated protoplasts of an s-(2-aminoethyl)-l-cysteine (aet-cys)-resistant cell line of pearl millet. initial selection for putative hybrids was performed by plating protoplast-derived microcolonies onto medium containing 300 mug of aet-cys per ml. calli growing in the presence of aet-cys were screened with respect to their electrophoretic pattern ... | 1986 | 16593739 |
| differences in repeated dna sequences between callus and shoot tissue of sugarcane (saccharum officinarum l). | | 1986 | 3770795 |
| in vitro propagation of a saccharum officinarum (l.) and sclerostachya fusca (roxb.) a. camus hybrid. | callus induction and plant differentiation were obtained in an intergeneric hybrid of saccharum officinarum and sclerostachya fusca. the sub clones showed morphological variation. chromosome numerical variation was not observed but structural aberrations were noticed in some sub clones. the study indicates the use of tissue culture technique for inducing intergeneric gene transfer in saccharum hybrids. | 1984 | 24258543 |
| plant regeneration via somatic embryogenesis in sugarcane. | plants, regenerated from callus cultures of sugarcane (saccharum officinarum l.) clone ij76-316, originated through somatic embryogenesis. callus cultures were established from primordial leaves and apical meristems on murashige and skoog medium (ms) supplemented with 3 mg 1(-1) 2,4-dichlorophenoxy acetic acid and 100 ml 1(-1) coconut water (msc3). nodular calli formed within 2 weeks of culture. calli were maintained on msc3 medium by transfer every 3 to 4 weeks. somatic embryogenesis occurred a ... | 1983 | 24257849 |
| purification and properties of thermostable beta-xylosidase from immature stalks of saccharum officinarum l. (sugar cane). | thermostable beta-xylosidase was purified from immature sugar cane stalks to an electrophoretically homogeneous form by ammonium sulfate fractionation, ion-exchange chromatography on deae-cellulose and p-cellulose columns, heat treatment (70 degrees c, 20 min) and gel filtration on a sephadex g-100 column. the purification was about 165-fold in specific activity with a high recovery of 43%. the apparent molecular weight of the enzyme, as determined by gel filtration, was 62,000. in sds-polyacryl ... | 1982 | 6819293 |