| mobilization of intracellular calcium by methacholine and inositol 1,4,5-trisphosphate in rat parotid acinar cells. | in the rat parotid acinar cell, methacholine caused an increase in [ca2+]i as determined by quin-2 fluorescence. the increase in [ca2+]i was initially independent of, and subsequently dependent on, the presence of extracellular ca2+, indicating mobilization of intracellular ca2+, as well as activation of ca2+ entry. methacholine mobilization of the internal ca2+ pool and stimulation of the initial transient phase of k+ efflux have similar concentration dependencies; the ec50 value for ca2+ mobil ... | 1987 | 3305630 |
| antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups a and b: evaluation of the contributions of f and g glycoproteins to immunity. | the degree of antigenic relatedness between human respiratory syncytial virus (rsv) subgroups a and b was estimated from antibody responses induced in cotton rats by respiratory tract infection with rsv. glycoprotein-specific enzyme-linked immunosorbent assays of antibody responses induced by rsv infection demonstrated that the f glycoproteins of subgroups a and b were antigenically closely related (relatedness, r approximately 50%), whereas the g glycoproteins were only distantly related (r app ... | 1987 | 3305988 |
| bubonic plague. | | 1987 | 3308795 |
| [a personal view of the history of the genus yersinia]. | the first recorded experience australia had of the genus yersinia was the arrival in 1889 of a french expedition led by pasteur's nephew, dr. adrien loir. at that time australia was in the grips of an epidemic of rabbits, and loir's purpose was to eradicate the rabbits by means of fowl plague (pasteurella multocida). sadly, bureaucratic and political obstacles prevailed, and loir was never granted permission to release his biological control agent. alexander yersin had been tempted to join loir' ... | 1987 | 3311629 |
| radioimaging of malignant melanoma xenografts in nude mice using 111in-labeled monoclonal antibody against human malignant melanoma. | | 1987 | 3312335 |
| concentrations of antibodies in paired maternal and infant sera: relationship to igg subclass. | previous studies comparing igg subclass concentrations in cord and maternal sera have indicated that igg1 is transported across the placenta to a greater extent than is igg2. the purpose of our study was to examine the relationship between the transport of igg1 and igg2 and the transport of specific antibodies that are relatively restricted to a particular subclass, either igg1 or igg2. the concentrations of total serum igg1 and igg2 and those of igg-anti-tetanus toxoid (tt) and anti-group a str ... | 1987 | 3312554 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| [serological reaction systems using enzyme-labelled immunospecific reagents]. | different serological test systems, based on the use of enzyme-labeled immunospecific reagents and intended for testing the material under study for the presence of yersinia pestis capsular antigen and antibodies to it, are described. comparative data on the evaluation of their sensitivity to the antigen and antibodies to it in different schemes of enzyme immunoassays (eia) are presented. as shown in this investigation, eia systems for the detection of the antigen and antibodies to it can compri ... | 1987 | 3318229 |
| diisopropanolnitrosamine (dipn) induced rat thyroid lesions. iii. an immunohistochemical study on their functional aspects. | the functional aspects of dipn induced rat thyroid lesions classified as in our previous studies were analyzed by immunohistochemical detection of tg, t4, and t3 in serial or semi-serial paraffin sections. the type 1 lesions, i.e., foci of cellular alteration of individual follicles, showed the same pattern of immunostaining as seen in the surrounding hyperplastic follicles and in normal controls. on the other hand, the type 3 lesions, i.e., carcinomas, were mostly negative for t4 and t3 with on ... | 1987 | 3318273 |
| [medico-genetic studies of human provisional organs]. | | 1987 | 3318529 |
| anti-candida drugs--the biochemical basis for their activity. | the past years have seen a continuous effort toward the synthesis of new antifungal agents. most of them belong to the n-substituted imidazoles and triazoles. another interesting series of antifungals are the allylamines. biochemically, both the azole derivatives and the allylamines belong to the class of ergosterol biosynthesis inhibitors and thus differ from the polyene macrolide antibiotics. indeed, it is now believed that the antifungal action of the polyenes, nystatin and amphotericin b, is ... | 1987 | 3319421 |
| [experience with homemade substrates for mycology]. | the meaning of the words "home-made", "medium" and "soil" is defined. the formulae are given and the manner for preparing lactritmel, lacmel and tritmel is described. the same for horse and cow dung agar soils is done. the lactritmel (20 fold diluted in water) is recommended for the specific diagnosis of candida albicans in its nascent culture. the first results are communicated, as obtained by slide culturing fungi on banana (musa paradisiaca) fruit pulp without previous sterilization. the amus ... | 1987 | 3320629 |
| evidence that dna involved in the expression of nodulation (nod) genes in rhizobium binds to the product of the regulatory gene nodd. | in rhizobium leguminosarum biovar viciae, the regulatory nodulation nodd gene has at least two functions. it constitutively represses its own transcription and in the presence of inducer flavonoid molecules, it activates the expression of two other nod gene transcriptional units, nodabcij and nodfe. upstream of noda and nodf is a conserved sequence, the nod box, which has been implicated in nodd-mediated transcriptional activation of these genes. dna fragments spanning the nod boxes that precede ... | 1987 | 3320955 |
| the aetiology of jaundice in adult patients at port moresby general hospital, papua new guinea. | | 1987 | 3321750 |
| cellular and subcellular distribution of exogenously administered renal renin in rat liver and kidney. | using highly purified 125i-labeled rat renal renin, we have demonstrated that both the liver and kidney are responsible for clearance of renal renin. in the present study, light and electron microscope autoradiography of intravenously administered 125i-labeled rat renal renin was performed in rat liver and kidney to observe the cellular and subcellular distribution of the renin. fifteen and 60 min after the injection, the liver and kidneys were removed and fixed with 0.1 m sodium phosphate buffe ... | 1987 | 3322039 |
| fetal heart rate, arterial pressure, and blood volume responses to cortisol infusion. | the purpose of this study was to determine whether physiological amounts of cortisol affect the fetal cardiovascular system. cortisol (4 micrograms/min) or the vehicle was infused intravenously for 5 h into six chronically catheterized sheep fetuses at 127-143 days gestation (term = 145-150 days). in the cortisol-infused animals, plasma cortisol concentration increased from 2.0 +/- 0.6 (se) to 8.3 +/- 0.9 ng/ml. there was a concomitant decrease in fetal heart rate of 38 beats/min (p less than 1 ... | 1987 | 3322047 |
| mode of action of nitro-heterocyclic compounds on escherichia coli. | the inhibitory and bactericidal activities of several different nitro-heterocyclic compounds, such as nitrofuran, nitronaphthofuran, nitrobenzofuran, nitroimidazole and nitrothiazole, were assessed in vitro. all these substances except nitroimidazole were active against escherichia coli, though to different degrees. under anaerobic test conditions the antibacterial activity increased slightly. nitroreductase-deficient mutants, however, were highly resistant to all nitro-compounds, indicating tha ... | 1987 | 3322751 |
| [frequency of appearance of persistent bvd virus infections and their effects on the cattle population]. | | 1987 | 3322777 |
| nonenzymatic oxidative cleavage of peptide bonds in apoprotein b-100. | incubation of low density lipoprotein (ldl) with endothelial cells converts it to a form that is avidly degraded by macrophages via the acetyl ldl receptor. this modification has previously been shown to be accompanied by extensive breakdown of the major ldl protein (apob-100) to smaller peptides. apob-100 is known to undergo partial degradation during isolation and purification which is commonly attributed to proteolytic enzymes derived from plasma or to contaminant bacteria. in the present stu ... | 1987 | 3323390 |
| immunohistochemical localization of metallothionein in cell nucleus and cytoplasm of fetal human liver and kidney and its changes during development. | the distribution of metallothionein (mt) during human development was investigated using both immunohistochemical and biochemical methods. the level of mt in the fetal liver was higher than the adult liver levels. higher levels of zinc (zn) and copper (cu) were also detected in the fetal liver compared to the adult liver. although cadmium (cd) was present in detectable levels in the human adult liver, none was detected in the human fetal liver. mt was localized in the nucleus and the cytoplasm o ... | 1987 | 3324023 |
| [dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin]. | kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ... | 1987 | 3324953 |
| [dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin]. | kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ... | 1987 | 3324953 |
| [chromosome stability in saccharomycete yeasts]. | mutants with high instability of chromosome iii designated chl+ (chromosome loss) were obtained after irradiation with uv the z4221-3c1 haploid disomic for chromosome iii. the chl+ mutants can be divided into two classes: 1) cl2, cl3, cl7, cl9, cl11, cl12, cl13 with elevated level of spontaneous inter- and intragenic recombination; 2) cl4, cl8 which unstable maintenance of chromosome iii not accompanied with elevation of mitotic recombination frequency. the cl4 and cl8 mutants also reveal, in co ... | 1987 | 3326785 |
| canine lymphoma: clinical considerations. | | 1986 | 3333887 |
| canine lymphoma: clinical considerations. | | 1986 | 3333887 |
| conjugative clearance of 1-naphthol and disposition of its glucuronide and sulfate conjugates in the isolated perfused rat kidney. | extensive extrahepatic metabolism of 1-naphthol (1-n) was shown to occur in the rat in vivo (clearance = 377 ml/min/kg). the renal contribution to the metabolism of 1-n was investigated in the isolated perfused rat kidney (ipk). 1-n was metabolized to its sulfate (1-ns) and glucuronide (1-ng) conjugate by the ipk. the renal clearance of 1-n ranged from 5.36 to 6.24 ml/min/kidney and was 80 to 95% of the p-aminohippuric acid clearance by the ipk. this indicated that the renal extraction ratio of ... | 1988 | 3336003 |
| conjugative clearance of 1-naphthol and disposition of its glucuronide and sulfate conjugates in the isolated perfused rat kidney. | extensive extrahepatic metabolism of 1-naphthol (1-n) was shown to occur in the rat in vivo (clearance = 377 ml/min/kg). the renal contribution to the metabolism of 1-n was investigated in the isolated perfused rat kidney (ipk). 1-n was metabolized to its sulfate (1-ns) and glucuronide (1-ng) conjugate by the ipk. the renal clearance of 1-n ranged from 5.36 to 6.24 ml/min/kidney and was 80 to 95% of the p-aminohippuric acid clearance by the ipk. this indicated that the renal extraction ratio of ... | 1988 | 3336003 |
| biofragmentable bowel anastomosis ring: comparative efficacy studies in dogs. | three studies were conducted in a total of 178 dogs to determine the efficacy of a biofragmentable bowel anastomosis ring (bar) composed of polyglycolic acid and 12.5% barium sulfate. wound strength and healing of bar, suture, and staple colonic anastomoses were compared for intervals of up to 1 year. the effect of systemic steroid treatment and bar size on anastomotic wound strength and healing was also studied. the bars fragmented at a mean time of 15.06 days postoperatively and were passed in ... | 1988 | 3336869 |
| the cpg dinucleotide and human genetic disease. | reports of single base-pair mutations within gene coding regions causing human genetic disease were collated. thirty-five per cent of mutations were found to have occurred within cpg dinucleotides. over 90% of these mutations were c----t or g----a transitions, which thus occur within coding regions at a frequency 42-fold higher than that predicted from random mutations. these findings are consistent with methylation-induced deamination of 5-methyl cytosine and suggest that methylation of dna wit ... | 1988 | 3338800 |
| identification and mapping of the temperature-inducible, plasmid-encoded proteins of yersinia spp. | the structural genes of the outer membrane polypeptides of yersinia spp. (yops) and the v antigen of plasmid pib1 of yersinia pseudotuberculosis were recently cloned and mapped (a. forsberg, i. bölin, l. norlander, and h. wolf-watz, microb. pathogen. 2:123-137, 1987). the corresponding genes were localized on pyv019 and pyv8081 of yersinia pestis and yersinia enterocolitica, respectively. no obvious differences were observed on comparison of pib1 and pyv019, whereas pyv8081 showed intragenic as ... | 1988 | 3338844 |
| analysis of cellular expression of gangliosides by gene transfection. i: gd3 expression in myc-transfected and transformed 3y1 correlates with anchorage-independent growth activity. | transfection of c-myc dna into rat fibroblastic 3y1 cell line resulted in the neosynthesis of gd3 ganglioside, as has been previously shown to occur after transfection of 3y1 cells with adeno el (nakakuma, h., sanai, y., shiroki, k., and nagai, y. (1984) j. biochem. 96, 1471-1480); in both cases, the products are expressed intranuclearly. moreover, a clear correlation was identified between levels of gd3 expression and colony-forming activity (in soft agar) of myc-transformed 3y1 cells, implying ... | 1988 | 3342047 |
| lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro. | the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ... | 1988 | 3345436 |
| c-h-ras and c-k-ras gene hypomethylation in the livers and hepatomas of rats fed methyl-deficient, amino acid-defined diets. | the extent of methylation of the c-h-ras and c-k-ras oncogenes was compared in neoplastic and preneoplastic livers of rats fed one of several methyl-deficient, amino acid-defined diets for 18 months, with or without a preceding initiating dose of diethylnitrosamine (den). the restriction endonucleases mspi, hpaii, hhai, paer71 and xhoi were used for studying the extent and pattern of dna methylation. the results indicated that both c-h-ras and c-k-ras oncogenes were hypomethylated in all dna sam ... | 1988 | 3345576 |
| cell death in the inner and outer nuclear layers of the developing cat retina. | the occurrence and distribution of pyknotic profiles (dying cells) in the inner (inl) and outer nuclear layers (onl) were mapped from radial sections through 13 kitten retinae aged from the 30th postconceptional day (30pcd) to the 81pcd (17th postnatal day). cell death in the inl occurs in two consecutive waves, each beginning at the area centralis and proceeding toward the retinal edge. the first wave involves cells located in the inner part of the inl, adjacent to the inner plexiform layer. it ... | 1988 | 3346373 |
| [transient improvement of the west syndrome in two cases following rotavirus colitis]. | | 1988 | 3348921 |
| a direct in vivo comparison of the inflammatory properties of human c5a and c5a des arg in human skin. | c5a is an 11,000-da complement-derived inflammatory glycoprotein that has been shown to mediate inflammatory reactions in vitro as well as in vivo in human skin. the c5a degradation product, c5a des arg, is rapidly formed after exposure of c5a to serum carboxypeptidase n and may represent the relevant c5-derived inflammatory peptide in vivo. to examine the biologic activity of human c5a des arg in vivo and to compare it with that seen with human c5a, we purified and characterized homogeneous pre ... | 1988 | 3351304 |
| rat-bite fever in a gerbil breeder. | a case of rat-bite fever caused by streptobacillus moniliformis in a gerbil-breeder is described. such a case has not been previously recorded. | 1988 | 3351317 |
| ascorbic acid specifically increases type i and type iii procollagen messenger rna levels in human skin fibroblast. | in cultured human skin fibroblasts, ascorbic acid stimulates collagen production with no apparent change in the intracellular degradation of newly synthesized procollagen. to understand the basis for this effect, we measured the steady-state levels of type i and type iii procollagen mrnas in cells treated with ascorbic acid. a three- to fourfold increase in collagen synthesis was associated with a two- to threefold increase in the levels of mrnas for both type i and type iii procollagens. these ... | 1988 | 3351329 |
| anti-inflammatory activity of oleoresin from brazilian copaifera. | the oleoresin from brazilian copaifera species yielded copalic acid and sesquiterpenes and showed marked anti-inflammatory activity using various experimental models in rats. the oleoresin significantly inhibited carrageenin-induced pedal edema following oral doses from 0.70 to 2.69 ml/kg, but was somewhat less effective than 50 mg/kg calcium phenylbutazone. repeated administration of the oleoresin at a dose of 1.26 ml/kg for a 6-day period reduced granuloma formation with a response comparable ... | 1988 | 3352280 |
| decrease in plasma tryptophan after tryptophan-free amino acid mixtures in man. | male healthy subjects, fasting 12 hours, ingested increasing amounts of a mixture containing a fixed proportion of seven essential amino acids (l-isoleucine 11.5%, l-leucine 18.0%, l-lysine 13.1%, l-methionine 18.0%, l-phenylalanine 18.0%, l-threonine 8.2%, l-valine 13.1%) and lacking tryptophan. the diets produced a rapid fall in plasma tryptophan which was proportional to the total amount of the amino acids ingested. following the highest dose administered (36.6 g) plasma tryptophan fell to a ... | 1988 | 3352467 |
| chorioamnionitis: a study of organisms isolated in perinatal autopsies. | the purpose of this study was to determine the validity of postmortem cultures in perinatal autopsies; in particular, those cases where there is gross or histologic evidence of chorioamnionitis. lung, liver, blood, and placental cultures were obtained from 159 neonatal autopsies with histologic evidence of chorioamnionitis at magee-women's hospital between january 1980 and july 1985. the criterion for chorioamnionitis was a polymorphonuclear leukocytic infiltrate in the placental membranes. the ... | 1988 | 3355095 |
| artificial insemination with fresh semen in beagle bitches. | | 1988 | 3361726 |
| effects of age on the motor unit. a study on single motor units in the rat. | | 1988 | 3364890 |
| species-specific oligonucleotide probes for the identification of human filarial parasites. | species-specific oligonucleotide probes have been constructed for the filarial parasites brugia malayi and brugia pahangi. both parasites contain a 322 base pair repeated dna sequence that is cleaved once by the restriction endonuclease hhai. a consensus repeat sequence was determined from the dna sequence of 15 cloned isolates of each species. although the two repeats have an average homology of 89%, half the differences are clustered in a region of 66 nucleotides that has a homology of only 72 ... | 1988 | 3367934 |
| activity of the anorectic agent 1,3-bis[2-cyano-5-(trifluoromethyl)-phenyl]triazene in in vitro and in vivo liver promoting assays. | a substituted 1,3-diaryltriazene, 1,3-bis[2-cyano-5-(trifluoromethyl)phenyl]triazene (bpt), was studied for promoting activity in vitro and in vivo. bpt inhibited intercellular molecular exchange between cultured hepatocytes and rat liver epithelial cells, although the effect was not consistent. for the in vivo assay, male f344 rats were first exposed to n-2-fluorenylacetamide (faa) for 8 weeks to induce liver altered foci, after which those maintained on control diet for an additional 12 weeks ... | 1988 | 3370626 |
| vasopressin and sympathetic nervous functions both contribute to development and maintenance of hypertension in doca-salt rats. | we evaluated the time course of changes in the relative contribution of arginine-vasopressin (avp) and sympathetic nervous system (sns) during the development of doca-salt hypertension in rats. intravenous administrations of an avp antagonist (d(ch2)5tyr(me) avp; avpa) and hexamethonium (c6) were given at 3 and 7 days, and 2 and 4 weeks after the initiation of treatment with doca-salt to conscious and unrestrained rats. mean arterial pressure (map) was higher and heart rate (hr) tended to be rap ... | 1988 | 3370846 |
| defect in cholesteryl ester transport in serum of patients with uremia receiving maintenance hemodialysis: increased inhibitor activity for cholesteryl ester transfer. | the rate of cholesterol transfer (rct) from high-density lipoproteins (hdls) to very low-density lipoproteins (vldls) and low-density lipoproteins (ldls) was previously found to be lower in the serum of patients with uremia receiving maintenance hemodialysis than in the serum of control subjects, and the defect was associated with the serum density greater than 1.063 gm/ml fraction (hsia et al. j lab clin med 1985;106:53). the present study shows that cholesterol transfer from hdls to vldls and ... | 1988 | 3373112 |
| susceptibility to yersinia pestis in the northern grasshopper mouse (onychomys leucogaster). | the laboratory-born progeny from two geographically distant populations of northern grasshopper mice (onychomys leucogaster) were challenged with yersinia pestis to determine their relative susceptibilities to plague. one of the o. leucogaster populations was associated with a known epizootic focus of the disease and was found to be nearly 2,000 times more resistant to mortality than were members of another population from an area historically free of plague. the ecology and omnivorous behavior ... | 1988 | 3373638 |
| arterial segmentation and subsegmentation in the human spleen. | the segmental nature of the arterial tree of the human spleen was analyzed in 181 subjects of both sexes, who had died of various accidental causes. based on the observation of the pattern of the terminal and polar splenic branches, selective arteriographs and corrosion casts, and taking account of the ideas reported in the literature, we proposed that the spleen is divided in arterial segments and subsegments. segments are the territories corresponding to both the primary branches of the spleni ... | 1988 | 3376732 |
| severe chlamydia trachomatis pneumonia in a patient with no immune deficiency. | a 40-year-old patient with no immune deficiency was admitted because of severe bilateral pneumonia and pleural effusion. the diagnosis of chlamydia trachomatis pneumonia was confirmed by isolation of the pathogen and the appearance of serum antibodies to chlamydia species. | 1988 | 3377617 |
| interaction of antiglucocorticoid ru 486 with rat kidney glucocorticoid receptor. | [3h]ru 486 competes with dexamethasone for rat kidney glucocorticoid receptor (gr) occupancy in vitro, exhibiting a higher association constant for binding to gr than [3h] dexamethasone. unlike [3h]dexamethasone-receptor complexes which dissociate rapidly at 37 degrees c even in the presence of molybdate, [3h]ru 486-receptor complexes remained more stable both in the presence and in the absence of molybdate. interestingly, sulfhydryl reagents such as n-ethylmaleimide, iodoacetamide and tosyllysy ... | 1988 | 3377791 |
| yersinia pestis: a case of mistaken identity. | | 1988 | 3380589 |
| the defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion. | this paper reports a study of the locomotor behaviour of the lymphocytes from 17 patients with chronic lymphocytic leukaemia (cll). the cells were studied both immediately after separation from blood and after culture for 24 to 48 h with a range of growth activators. cells direct from blood were tested for polarization in fetal calf serum (fcs 20%), phorbol myristate acetate (pma 10(-7)m) and colchicine (10(-5)m). the polarization of lymphocytes from cll patients with high white cell (wbc) count ... | 1988 | 3383451 |
| the correlation between plasma gastrin levels and abomasal acid secretion in cows. | | 1988 | 3386108 |
| [ecological and biological maintenance of sapronoses exemplified by melioidosis]. | the growth and death of pseudomonas pseudomallei, the causative agent of melioidosis, in the soil and the antigenic properties of this microorganism in the soil, in culture media, and in the body of animals have been studied. as revealed in this study, p. pseudomallei can grow in nonsterile soil substrates without the loss of virulence and changes in its antigenic structure. in the body of animals this microorganism rapidly adapts its virulence to host species by the transformation of its antige ... | 1988 | 3389005 |
| [morphological study of the damaging effect of an eb vaccine strain of the plague microbe in inbred mice]. | the data obtained during the study of the morphological changes in the internal organs and blood of 6 mouse strains at the early stages of anti-plague immunity are presented. it has been established that the introduction of 5.10(3) and 1.10(5) m. b. of the ev vaccine strain resulted in the development of morphological changes typical of residual virulence. the degree of these changes depended on the haplotype of the animals and the dose used. the most resistant to injury induced by ev strain wer ... | 1988 | 3390604 |
| morphology and contractile properties of smooth muscle cells isolated from the dog carotid artery. | collagenase and elastase treatment was used to isolate vascular smooth muscle cells from canine carotid artery. their structure and function were compared to those in situ. morphological studies showed that these cells when relaxed in situ were 120-133 micron mean length, connected by numerous typical gap junctions, covered by a basal lamina and like other smooth muscles in structure. after isolation, the median length of single cells was 82 micron. there was structural evidence of some contract ... | 1988 | 3390615 |
| micromanipulation in human reproductive technology. | | 1988 | 3396693 |
| anabolic effects on rate, composition and energetic efficiency of growth in cattle fed forage and grain diets. | the effects of anabolic implants on rate, composition and energetic efficiency of growth were determined in steers fed diets varying in forage and grain content. santa gertrudis-cross steers averaging 337 kg were group-fed (n = 72) or individually fed (n = 45) ad libitum one of three diets and either not implanted or implanted (90-d intervals) with ralgro or synovex-s implants. steers were fed to a similar empty body weight (463 kg). initial empty body composition of individually fed steers was ... | 1988 | 3403412 |
| the a- and b-chains of carboxypeptidase i from germinated barley originate from a single precursor polypeptide. | carboxypeptidase i from germinated barley (hordeum vulgare) grain consists of two peptide chains linked by disulfides; the a- and b-chains contain 266 and 148 amino acid residues, respectively (sorensen, s. b., breddam, k., and svendsen, i. (1986) carlsberg res. commun. 51, 475-485). a cdna library prepared from mrna isolated from scutella of 2-day germinated barley has now been screened with a mixed oligonucleotide encoding a peptide fragment of the a-chain. nucleotide sequence analysis of a 14 ... | 1988 | 3403516 |
| evolution of plague virulence. | | 1988 | 3405295 |
| effect of pregnancy on ritodrine pharmacokinetics. | dosing regimens for ritodrine are based in large part on pharmacokinetic studies performed with nonpregnant subjects. pregnancy is characterized by changes in renal blood flow, plasma volume, protein concentration, and hepatic function. these physiologic changes frequently alter drug pharmacokinetics. to define the effect of pregnancy on ritodrine kinetics, we compared ritodrine pharmacokinetics in four pregnant and four nonpregnant rhesus monkeys. significant differences were demonstrated in th ... | 1988 | 3407687 |
| expression of fibronectin on clonally related transformed and control sublines from an epithelial cell strain and a tumor line of mouse alveolus. | the cellular expression of fibronectin and the importance of fibronectin for the morphology of cultured cells were studied in a lung epithelial cell system. the cells used were clonally related control nal1a, spontaneously transformed nal1as cells of the nal1a cell strain, and transformed clonal sublines of the cell line nal1am and the tumor cell line nul1. fibronectin was detected on the surface of nal1a cells by surface iodination; fibronectin synthesis, secretion, and pericellular accumulatio ... | 1988 | 3409226 |
| low-frequency ultrasound absorption in aqueous solutions of hemoglobin, myoglobin, and bovine serum albumin: the role of structure and ph. | new measurements on ultrasound absorption of aqueous solutions of the three globular proteins, bovine hemoglobin, horse heart myoglobin, and bovine serum albumin, at 20 degrees c are reported for the frequency range below 1.2 mhz and, in the case of hemoglobin and bovine serum albumin, to a limited extent for the range 2-30 mhz. the effect of protein conformation has been investigated by use of a range of denaturants and by change of ph. a much more detailed description of protein interactions e ... | 1988 | 3411029 |
| a plague epizootic in the white-tailed prairie dogs (cynomys leucurus) of meeteetse, wyoming. | surveillance for sylvatic plague (yersinia pestis) was conducted near meeteetse, wyoming (usa) from 24 may to 14 june 1985. ten species of fleas were collected from white-tailed prairie dogs (cynomys leucurus), and from their burrows and associated rodents. five of these flea species and two adult prairie dogs were positive for plague. the progression of this plague epizootic appeared to be slower and the intensity was less than in previous epizootics in other prairie dog colonies. the plague ep ... | 1988 | 3411698 |
| glutaraldehyde-preserved venous valve transplantation in the dog. | transplantation of femoral vein grafts was performed on 33 mongrel dogs to assess graft patency and valvular function after storage in glutaraldehyde. the grafts were removed from the donor, flushed with room temperature heparinized lactated ringer's solution, and then stored in a 0.2% glutaraldehyde solution for 16 hr. at the time of grafting, the veins were again flushed with lactated ringer's and anastomosed orthotopically to the recipient. an arteriovenous fistula was also created. postopera ... | 1988 | 3411953 |
| relation of changes in amount and type of dietary fat to fecapentaenes in premenopausal women. | correlation studies suggest that fecal mutagenicity is increased in groups eating high-fat diets, the same groups who are often found to have high colorectal cancer incidence and mortality. the fecapentaenes are the best characterized class of fecal mutagens, but the relationship of dietary fat intake to the excretion of these potent genotoxins is unknown. we studied the effect of changes in amount and type of dietary fat on fecapentaene levels in 31 premenopausal women 20-40 years of age who pa ... | 1988 | 3412371 |
| relation of changes in amount and type of dietary fat to fecapentaenes in premenopausal women. | correlation studies suggest that fecal mutagenicity is increased in groups eating high-fat diets, the same groups who are often found to have high colorectal cancer incidence and mortality. the fecapentaenes are the best characterized class of fecal mutagens, but the relationship of dietary fat intake to the excretion of these potent genotoxins is unknown. we studied the effect of changes in amount and type of dietary fat on fecapentaene levels in 31 premenopausal women 20-40 years of age who pa ... | 1988 | 3412371 |
| effects of temporal frequency on the contrast sensitivity of the human accommodation system. | contrast thresholds were determined for the initiation of accommodative responses to drifting sine-wave stimuli (4.1 c/deg) over a range of temporal frequencies (0-14.0 hz). accommodation was monitored with a dynamic infrared optometer. psychophysical detection thresholds were also determined for the same stimuli. a comparison of the contrast thresholds for detection with those for the initiation of accommodation indicates that these two tasks are mediated by mechanisms of comparable sensitivity ... | 1988 | 3414014 |
| experimental contact sensitization with 3,4,5-trichloropyridazine. | the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ... | 1988 | 3416588 |
| genetic characterization of leishmania isolates at 37 enzyme loci. | | 1988 | 3417374 |
| optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes. | four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ... | 1988 | 3417404 |
| spinal cord penetration of intrathecally administered cytarabine and methotrexate: a quantitative autoradiographic study. | carcinomatous meningitis is an increasingly common complication of systemic cancer. treatment usually involves the use of intrathecal methotrexate or cytarabine, which have been reported to be toxic to the spinal cord. this study was performed to determine the penetration and distribution of intrathecally administered chemotherapy within the spinal cord. tritiated methotrexate and cytarabine were administered intraventricularly and by lumbar puncture to new zealand white rabbits. the extent and ... | 1988 | 3418727 |
| the effects of aspirin-like drugs on the nutritional status of pregnant rats and offspring. | previous studies have shown that salicylates and protein-calorie malnutrition independently compromise maturation and growth of infants. in the present study, pregnant rats were fed normal-and low-protein diets with and without aspirinlike drug treatments. the effects of a low-protein (8 percent) diet included decreases in total serum proteins in both dams and their offspring. decreases in weight gains, serum albumin and globulin levels, and blood urea nitrogen (bun) concentrations were detected ... | 1988 | 3418736 |
| profile of patients from a veterans administration medical center. | data were analyzed to establish a general patient profile that could be the basis for specific profiles to be utilized for infectious disease and microbiologic studies. the analysis revealed that the average patient in this institution is a 57-year-old, white, married, protestant man residing in oklahoma.a personal-interview survey of 42 inpatients showed that 95 percent had smoked for a number of years, and of the smokers, 60 percent were current smokers while the remaining 40 percent had stopp ... | 1988 | 3418737 |
| virus-pesticide interactions with murine cellular immunity after sublethal exposure to dieldrin and aminocarb. | interaction of two potential immunosuppressive factors, sublethal pesticide exposure and viral inhibition of lymphocyte mitogenesis, was examined in mixed lymphocyte reaction (mlr). inbred (c57bl/6 x a/j)f mice, semisusceptible to mouse hepatitis virus 3 (mhv3) infection were exposed to selected pesticides and subsequently infected with the mhv3 virus. the mortality of animals was examined as a function of pesticide exposure. two pesticides were selected for further studies: the organochlorine p ... | 1988 | 3418741 |
| common genetic pools between human populations. | a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ... | 1988 | 3422217 |
| native genomic blotting: high-resolution mapping of dnase i-hypersensitive sites and protein-dna interactions. | dnase i-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. we have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic dna on native polyacrylamide gels prior to electroblotting and hybridization. this improved method has been used to scan the promoter and coding region of a cell-cycle-dependent human histone h4 gene with an accuracy of +/-5-1 ... | 1988 | 3422413 |
| plague meningitis--a retrospective analysis of cases reported in the united states, 1970-1979. | meningitis caused by yersinia pestis developed in 6 (6%) of a total of 105 patients with plague reported to the centers for disease control from 1970 to 1979. five of the six cases occurred in children aged 10 to 15 years. all six patients received antibiotic therapy before meningitis developed, which appeared between the 9th and 14th days after the onset of acute illness in five of the six patients. there were no neurologic sequelae. the antigenic and biochemical profiles of the y pestis strain ... | 1987 | 3424819 |
| interactions between host cells and yersiniae. | | 1986 | 3426159 |
| [comparative studies of humane electric stunning of different types of poultry for slaughter]. | | 1987 | 3426532 |
| ophthalmic bioequivalence of steroid/antibiotic combination formulations. | this study compared the relative ocular bioavailability in rabbits of the antibiotic/steroid combination of 0.3 per cent tobramycin and 0.1 per cent fluorometholone acetate to each of the two single-entity products. two separate studies were conducted, one measuring fluorometholone acetate in cornea and aqueous humour through 240 min and the other measuring tobramycin in the cornea through 120 min. the results for fluorometholone acetate show that the combination product is 15 per cent higher in ... | 1987 | 3427210 |
| monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane. | an accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented which involves the use of 18o-labeled internal standards. the method is employed for the determination of hydroperoxides in rat liver microsomes after aerobic incubation with fe2+/adp and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. the amounts found after artificial microsomal "lipid peroxidation" are almost two orders of magnitude large ... | 1987 | 3431344 |
| a rapid and safe procedure for preparing the murine toxins of the plague bacillus (yersinia pestis). | the murine toxins a and b of y. pestis were purified from a crude extract of the nonencapsulated strain m23np of y. pestis using hplc gel filtration. toxins a and b had molecular weights of 240,000 and 120,000, respectively. both toxins had isoelectric points of 3.8. the mouse ld50 of toxin a was 2.5 micrograms, that of toxin b was 1.8 micrograms. of the protein injected on the column, approximately 9% was toxin, including 2.9% toxin a and 6.1% toxin b. the crude m23np extract was used periodica ... | 1987 | 3433308 |
| [numerical analysis of the phenotypic properties and total genomic characteristics of strains of yersinia pestis related to different subspecies]. | the numerical analysis of the phenotypical properties of y. pestis strains, classified with 5 subspecies by 60 signs, was carried out. in comparing the properties of strains belonging to the main (nomenclature) subspecies with strains of other subspecies, the similarity index varied within the range 82-95%. a high degree of genetic affinity between 21 y. pestis strains of five subspecies was demonstrated by the method of molecular dna-dna hybridization. the level of dna homology with respect to ... | 1987 | 3434043 |
| enzyme activity changes in rat soleus motoneurons and muscle after synergist ablation. | quantitative enzyme histochemical methods have been used to determine the effect of ablation of synergists on the oxidative metabolism of the alpha-motoneurons and muscle fibers of the rat soleus. sixty days postablation, the nadh-tetrazolium reductase (nadh-tr) activity of soleus motoneurons decreased 12.5% from 0.327 +/- 0.005 (mean +/- se; optical density units) to 0.286 +/- 0.007. in the muscle fibers, the alpha-glycerophosphate dehydrogenase activity (glycolytic enzyme) decreased from 0.114 ... | 1987 | 3436865 |
| [involvement of the growth hormone in rapid increases of the potassium concentration of the serum]. | as a rule, the infusion of arginine hydrochloride is followed by an increase in serum potassium. the investigation is concerned with the short-term effect of growth hormone, which can be stimulated by arginine (0.6 g/kg body weight), on potassium concentrations in rats and patients with cerebral death. the administration of human somatotropin causes a significant rise in potassium concentrations in patients with cerebral death (0.4 iu/kg body weight) and in rats (4.0 iu/kg body weight). in compa ... | 1987 | 3439594 |
| tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells. | when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ... | 1987 | 3440028 |
| effects of pomc-derived peptides on aldosterone secretion in vivo. | 1. to investigate a role for peptides derived from the precursor molecule pro-opiomelanocortin (pomc) on the control of aldosterone secretion (asr), alpha-, beta-, gamma 1-, and gamma 2-melanocyte stimulating hormone (msh), corticotropin-like intermediate lobe peptide (clip) or beta-endorphin were infused into the adrenal arterial supply of sheep with an adrenal cervical autotransplant. 2. none of the peptides had any significant effect on aldosterone secretion rate in na replete, unstressed, co ... | 1987 | 3440325 |
| genetic mapping of eleven spo genes essential for ascospore formation in the fission yeast schizosaccharomyces pombe. | sporulation-deficient mutants of the fission yeast schizosaccharomyces pombe were isolated from a homothallic strain mutagenized with ethyl methanesulfonate. complementation tests defined two new genetic loci (spo19 and spo20) essential for ascospore formation, in addition to the 18 known spo loci (bresch et al. 1968). a novel mapping procedure using random spore analysis prior to tetrad analysis allowed us to map 11 spo genes. four genes (spo3, spo15, spo19 and spo20) were mapped on chromosome ... | 1986 | 3442824 |
| [research on yersinia pestis in rodents and other small animals in a plague focus of the northeastern brazil from 1966 to 1982]. | | 1987 | 3445109 |
| [adenylate cyclase of the causative agent of plague: its purification and properties]. | three forms of adenylate cyclase have been detected in y. pestis: membrane-bound, cytoplasmic and extracellular. extracellular adenylate cyclase has been purified so as to achieve a homogeneous state, and some of its physicochemical parameters have been investigated. in the process of purification the initial preparation of this enzyme has been subjected to heating at 100 degrees c for 15 minutes, fractionation with ammonium sulfate, and gel filtration on sephadex g-100. the homogeneity of adeny ... | 1987 | 3445726 |
| [use of erythrocyte diagnostic agents in the universal system of serological reactions]. | a method of parallel serologic studies of the material for the detection of specific antigen or antibodies is proposed. this method is not inferior to the known systems of serologic tests (passive hemagglutination-antigen neutralization tests, passive hemagglutination--antibody neutralization tests, etc) in sensitivity and economy, but exceeds all of them in informative value. the method has been tested both experimentally and in the epizootic study of plague infection used as a model. a serolog ... | 1987 | 3445733 |
| lactation response to short-term abomasal infusion of choline, inositol, and soy lecithin. | five lactating holstein cows averaging 13 wk postpartum were used in a latin square design to examine the effect of daily abomasal infusion of choline (22 g), myo-inositol (37 g), soy oil (325 ml), or crude soy lecithin (900 ml) on lactation performance. dry matter intake was reduced by infusion of soy lecithin as compared with infusion of water (18.1 and 21.1 kg/d, respectively). plasma beta-hydroxybutyrate concentration was increased when cows received the myo-inositol or soy lecithin infusion ... | 1987 | 3448106 |
| t-2 toxemia and brain prostaglandins. | t-2 toxin is a trichothecene mycotoxin which is a member of a family of closely related sesquiterpenoids. it was recently shown that t-2 toxemia is associated with elevated plasma levels of eicosanoids. to study further the effect of t-2 on the cyclooxygenase pathway of arachidonate we examined the release of pge2, txb2 and 6-keto-fgf1 alpha from brain tissue exposed to t-2 toxin in vivo or in vitro. administration of t-2 toxin (0.75 or 2 mg/kg) to conscious rats caused a transient increase in t ... | 1986 | 3457405 |
| active smoking depresses prostaglandin synthesis in human gastric mucosa. | to determine the effect of smoking on gastroduodenal mucosal prostaglandin synthesis, endoscopies were done after an overnight fast on 10 nonsmokers, 12 active smokers who smoked four cigarettes in the hour before endoscopy, and then 11 of the smokers who refrained from smoking for 12 hours. biopsy samples of fundic, antral, and duodenal mucosae were incubated, and the accumulation of prostaglandin e2 and 6-keto-prostaglandin f1 alpha in the incubation medium was measured by radioimmunoassay. we ... | 1986 | 3457547 |
| replacement of 5-methylcytosine by cytosine: a possible mechanism for transient dna demethylation during differentiation. | in an earlier study it was discovered that when friend erythroleukemia cells (felc) were exposed to a variety of chemical agents capable of inducing differentiation, their dna underwent genome-wide transient demethylation. in an attempt to elucidate the biochemical mechanism responsible for this phenomenon we have induced felc with 5 mm hexamethylenebisacetamide and labeled the dna in vivo with a density label, 5-bromodeoxyuridine, and a radioactive label, deoxy[5-3h]cytidine. newly replicated d ... | 1986 | 3458243 |
| promotion of tubulin assembly by carboxyterminal charge reduction. | | 1986 | 3460425 |
| the bacteriology of sheep periodontitis with special reference to actinbacillus actinomycetemcomitans. | | 1986 | 3466409 |