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drug resistance and lecithinase activity of yersinia enterocolitica isolated from buffalo milk.two-hundred-and-seven samples of raw buffalo milk and 60 samples of pasteurized buffalo milk were screened for presence of yersinia enterocolitica. the prevalence of y. enterocolitica was found to be 24.1% in raw milk, however, no isolation could be made from the pasteurized milk samples. cold enrichment in trypticase soy broth and alkali treatment methods were followed in this study. the majority of the isolates (62%) were found sensitive to all the antibiotics used and only a few (16%) were re ...19892641492
the ail locus is found uniquely in yersinia enterocolitica serotypes commonly associated with disease.yersinia enterocolitica is a heterogeneous group of organisms with more than 50 serotypes and several biotypes. only a few of these serotypes cause gastrointestinal disease in otherwise healthy hosts; these serotypes are the pathogenic serotypes. although y. enterocolitica requires a high-molecular-weight plasmid to cause disease, chromosome-encoded determinants are required for the full expression of virulence. the ability of yersinia spp. to invade eucaryotic cells is thought to be a virulence ...19892642465
yersinia in intestinal biopsy specimens from patients with seronegative spondyloarthropathy: correlation with specific serum iga antibodies. 19892642517
yersinia antigens in synovial-fluid cells from patients with reactive arthritis.we examined synovial-fluid cells from 15 patients with reactive arthritis after yersinia infection for the presence of yersinia antigens. extensive bacterial cultures of the synovial fluid were negative. all the samples were studied by immunofluorescence with use of a rabbit antiserum to yersinia enterocolitica o:3 and a monoclonal antibody to y. enterocolitica o:3 lipopolysaccharide. synovial-fluid cells from 41 patients with other rheumatic diseases served as controls. synovial-fluid cells fro ...19892643047
homology between virf, the transcriptional activator of the yersinia virulence regulon, and arac, the escherichia coli arabinose operon regulator.virulent yersiniae (yersinia pestis, y. pseudotuberculosis, and y. enterocolitica) restrict their growth at 37 degrees c in rich medium deprived of calcium. this property, called calcium dependency, correlates with the secretion of yersinia outer membrane proteins (yops) and with pathogenicity. it is mediated by a 70-kilobase plasmid called pyv. the structural genes of the yops (yop genes), as well as genes involved in the control of their expression (vir genes), have been localized on pyv. in t ...19892644192
automated biochemical identification of bacterial fish pathogens using the abbott quantum ii.the quantum ii, originally designed by abbott diagnostics for automated rapid identification of members of enterobacteriaceae, was adapted for the identification of bacterial fish pathogens. the instrument operates as a spectrophotometer at a wavelength of 492.600 nm. a sample cartridge containing 20 inoculated biochemical chambers is inserted in the path of the analyzing beam. reactions are converted into a 7-digit octal biocode, relayed via a sensor to the memory module, and compared to biocod ...19892644450
[comparison of 2 methods of hemagglutination tests for antibodies against strains of yersinia enterocolitica and yersinia pseudotuberculosis].the authors compare results of the assessment of antibodies against bacterial strains of yersinia enterocolitica and yersinia pseudotuberculosis. the examinations were made by the haemagglutination technique. in the reaction of the antigen human and ram erythrocytes were used as carriers. the work comprised 462 examinations of patients with an assumed yersinia aetiology of the disease and 200 healthy controls. the values obtained during parallel examinations with antigens of yersinia enterocolit ...19892646030
evaluation of two rapid methods to screen pathogens from stool specimens.two rapid methods, the enteric pathogen screen (eps) cards of the automicrobic system (vitek systems, inc., hazelwood, mo) and the bactigen salmonella-shigella latex agglutination (lpa) method (wampole laboratories, cranbury, nj) were compared with conventional biochemical tests to screen stool cultures having suspicious colonial morphologic characteristics for the presence of salmonella and shigella in a study of 481 isolates from stool specimens and 104 stock cultures. compared with convention ...19892646912
[a 3-year evaluation of systematic research on salmonella, shigella, campylobacter and yersinia at a montpellier hospital].this work aims at assessing the prevalence of four bacterial genus that are the most frequently involved in infectious diarrhoea, among patients usually kept apart from this type of pathology (there is not a department of digestive tract diseases, of infectious diseases, of pediatry). among 3.626 stools received in our laboratory from 1.163 patients, 12 strains of campylobacter, 11 strains of salmonella and only one strain of yersinia were isolated. no strain of shigella was found. of the 12 str ...19892648269
liver abscess due to yersinia enterocolitica: case report and review of the literature. 19892649322
enzyme-capture assay for rapid detection of escherichia coli in oysters.enzyme-capture assays (ecas) for escherichia coli beta-d-glucuronidase (gud) were performed directly from 24-h gas-positive lauryl tryptose broth (ltb) fermentation tubes that had been inoculated with oyster homogenate seeded with e. coli. the ltb-eca method yielded results in 1 day that were equivalent to those obtained in 2 days by an ltb and ec-4-methylumbelliferyl-beta-d-glucuronide (ec-mug) method. overall, 62 of 64 (97%) positive ec-mug broths from which e. coli was isolated were correctly ...19892650619
nucleotide sequence of the plasminogen activator gene of yersinia pestis: relationship to ompt of escherichia coli and gene e of salmonella typhimurium.we have determined the nucleotide sequence of the 1.4-kilobase dna fragment containing the plasminogen activator gene (pla) of yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. the sequence revealed the presence of a 936-base-pair open reading frame that constitutes the pla gene. this reading frame encodes a 312-amino-acid protein of 34.6 kilodaltons and containing a putative 20-amino-acid signal sequence. the presence of a single large open re ...19892651310
[detection of plague microbes in fleas using immunoenzyme technics and monoclonal antibodies].the paper presents the results of immunoassays for indication of pest causative agents in fleas, this method being compared to the classical and bacteriological methods. experiments proved the feasibility of indication of pest causative agents in fleas by immunoassays. immunoassays were found to excel the classical and bacteriological ones.19892651856
bovine abortion associated with yersinia pseudotuberculosis infection. 19892652872
superior mesenteric vein thrombosis caused by yersinia pseudotuberculosis. 19892655810
evaluation of enzyme immunoassay for the detection of pathogenic yersinia enterocolitica and yersinia pseudotuberculosis strains.to determine the virulence plasmid-harboring strains of yersinia enterocolitica, we prepared antiserum against plasmid-encoded proteins of y. enterocolitica serotype o3 and carried out an enzyme immunoassay (eia) against temperature-inducible released proteins. this serum reacted with proteins released from not only a y. enterocolitica serotype o3 strain but also y. enterocolitica serotype o5:27, o8, and o9 and y. pseudotuberculosis serotype 1b, 2a, 2b, 2c, 3, 4a, 4b, 5a, 5b, 6, 7, and 8 strains ...19892656747
special features of distribution of yersinia pseudotuberculosis in japan.the incidence of yersinia pseudotuberculosis isolation from humans, animals, water, and pork in japan is presented on the basis of a review of the literature and serotyping of 633 strains referred to our laboratory. most of the strains belonged to serogroup 4b, followed in frequency of isolation by serogroups 3, 1b, 5b, 5a, and the others, whereas serogroup 1a has not been detected. although strains were isolated from 11 species of animals, none were isolated from birds.19892656752
analysis of iga antibodies to lipopolysaccharide in yersinia-triggered reactive arthritis. 19892656882
high degree of conservation between ribosomal proteins of yersinia pseudotuberculosis and escherichia coli. 19892657663
two outbreaks of yersinia pseudotuberculosis 5a infection in japan.in the summer of 1984 there occurred 2 outbreaks of infection with yersinia pseudotuberculosis 5a. 58 persons ate meat products, vegetables and rice at a japanese barbecue restaurant. 39/58 (67%) developed symptoms. in 19/39 persons an infection with y. pseudotuberculosis could be diagnosed by stool cultures and/or serological techniques. fever occurred in all patients, exanthema in 68% and abdominal pain in 68%. thus, human yersiniosis due to y. pseudotuberculosis 5a could be a febrile disease ...19892658021
liver abscesses due to yersinia enterocolitica in patients with haemochromatosis.liver abscesses due to yersinia enterocolitica usually occur in patients with a pathological iron overload. a case of multiple liver abscesses in a patient with haemochromatosis is reported and the literature is reviewed.19892659721
yersinia enterocolitica. 19892659991
the toxic effects of desferrioxamine.df has a low general toxicity, perhaps because of its low lipid solubility, kpart 0.01 (porter et al, 1988b). this feature of the molecule may prevent it from penetrating most cells of the body. it appears that there may be a specific mechanism of uptake of the drug by hepatocytes (porter et al, 1987), making the iron in these cells available for excretion via the bile, while the iron excreted in the urine may all come from extracellular chelation, particularly when iron leaves the reticuloendot ...19892660937
yersinia infections in surgical practice.this article reviews the clinical manifestations of yersinia bacteria emphasizing their recent rise in incidence and describing how yersinia infection presents to the general surgeon. geographical variations, patterns of disease, pathogenicity and the problems in diagnosis and management are discussed. this common but often unrecognized infection is usually self-limiting but the very young, the very old and the immunocompromised are at greater risk and prompt treatment is required to save lives.19892660950
thermoregulation-dependent expression of yersinia enterocolitica protein 1 imparts serum resistance to escherichia coli k-12.resistance to the bactericidal action of normal human serum is one of the characteristics of virulent yersinia enterocolitica. this property is attributable to the virulence plasmid harbored by pathogenic strains of the species. serum resistance in y. enterocolitica is thermoregulated, and its expression correlates well with the presence of virulence plasmid-encoded outer membrane proteins. to further examine the biochemical basis underlying resistance, we cloned a large segment (ca. 30 kilobase ...19892661534
ultrasonography and parameters of inflammation in acute appendicitis. a comparison with clinical findings.a prospective study of 110 unselected patients with suspected acute appendicitis comprised ultrasonography and parameters of inflammation--c-reactive protein, white blood cell count, erythrocyte sedimentation rate and yersinia antibody titer. the results were compared with the initial clinical diagnosis. acute appendicitis was confirmed in 28 cases (prevalence 25.4%). the negative laparotomy rate was 11.4% and the appendiceal perforation rate 10.7% clinical judgment and ultrasonography were stat ...19892662692
[yersinia enterocolitica infections and thalassemia major in children].we report two cases of severe yersinia enterocolitica infection in children with homozygous thalassemia. one patient had septicemia and the other had mesenteric adenitis. two factors can enhance the infectivity of yersinia enterocolitica in children with thalassemia: iron overload and deferoxamine therapy. laparotomy and cefotaxime-netilmicin therapy were successful in the patient with mesenteric adenitis. in the patient with septicemia, cefotaxime-netilmicin, then doxycycline-netilmicin failed, ...19892662880
yersinia pseudotuberculosis and arthritis.in 1986 and 1987 nine patients with a raised antibody titre to yersinia pseudotuberculosis were found. two of these results were almost certainly due to the cross reactivity between some y pseudotuberculosis serogroups and some salmonella serotypes. of the other seven patients with otherwise unexplained y pseudotuberculosis serology, three suffered from severe arthritis and two had symptoms suggestive of sacroiliitis. our data and published reports suggest that reactive arthritis might follow bo ...19892662919
yersinia enterocolitica in donor blood: a case report and review.routine sterility control of a unit of leukocyte-depleted erythrocyte concentrate yielded growth of yersinia enterocolitica serotype o:3. plasma of the donor showed a high titer of agglutinins against the homologous organism. although the donor was apparently well at the time of donation, he had a history of protracted terminal ileitis treated by surgery. the recipient of the contaminated blood was taking broad-spectrum antibiotics and did not experience any adverse effect. fourteen other cases ...19892663913
[cloning and study of the function of reca-like gene of yersinia pestis in escherichia coli cells].a 2 kb fragment of yersinia pestis genome cloned in escherichia coli cells of the strain hb101 contains a gene able to complement the reca-dependent deficiency of e. coli cells in uv-resistance, resistance to alkylating agents, uv- and mnng-induced mutability. cellular capability for homologous recombination in crosses with hfrh donor, derepressed synthesis of bacteriocins (colicin e1 and pesticin 1) is also complemented by the fragment in e. coli reca-strains. the obtained data suggest the func ...19892664489
[evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae].m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ...19892665580
partial purification and characterization of phospholipase c from yersinia enterocolitica.about 34% of the strains of yersinia enterocolitica isolated from raw milk were found to produce lecithinase. a selected strain produced phospholipase c at 22 degrees c and 37 degrees c; production was optimum at 37 degrees c in the stationary phase (14-16 h). a decrease in phospholipase c activity at various storage temperatures (-5 degrees c, 4 degrees c, 37 degrees c) was also observed, although the enzyme was active over a wide range of temperature (5-65 degrees c) and ph (3.5-7.5). the phos ...19892666377
[yersinia infection simulating acute appendicitis]. 19892666715
intestinal infections: current aspects and new possibilities of prophylaxis and treatment.intestinal infections of still high importance from the hygienic-epidemiological aspects and the recent results obtained in this field have been surveyed. recently recognized enteric pathogens (campylobacter, yersinia, rotavirus, clostridium difficile, etc.) as well as the newest data on the pathomechanism of intestinal infections have been discussed. these data not only have increased our knowledge of the pathology but also resulted in basic, favourable changes in therapy. detailed information ...19892667192
acute interstitial nephritis associated with yersinia pseudotuberculosis infection.we report two cases of acute interstitial nephritis associated with yersinia pseudotuberculosis infection. the patients had fever, abdominal pain, vomiting and acute renal failure coinciding with elevated agglutination antibody titer for y. pseudotuberculosis. renal biopsy revealed interstitial nephritis in both patients. although it is well known that yersiniosis sometimes affects glomeruli, this is the first report to demonstrate acute interstitial nephritis in patients with y. pseudotuberculo ...19892667363
chronic non-specific diarrhea of infancy successfully treated with trimethoprim-sulfamethoxazole.fifteen children who fulfilled the criteria of chronic non-specific diarrhea of infancy were evaluated for intestinal bacterial overgrowth. in 10 of 11 successfully investigated children we found bacterial overgrowth of the small intestine by upper respiratory tract microflora. in 9 of 10 children (group i) treated with trimethoprim-sulfamethoxazole the diarrhea ceased immediately, whereas in all children in group ii (n = 5; 3 children excluded because of growth of yersinia enterocolitica) treat ...19892669120
importance of intestinal mucosal immunity and luminal bacterial cell wall polymers in the aetiology of inflammatory joint diseases.the distal intestine contains bacterial cell wall polymers capable of inducing acute and chronic polyarthritis if systemically distributed. parenteral injection of peptidoglycan-polysaccharide (pg-ps) polymers from certain bacterial species produces spontaneously relapsing erosive synovitis in susceptible rat strains, and normally subarthropathic amounts of pg-ps and lipopolysaccharide (endotoxin) can reactivate arthritis initially induced by pg-ps. these experimental results illustrate the infl ...19892670253
the relationship between klebsiella infection and ankylosing spondylitis.ankylosing spondylitis (as) is probably produced by repeated episodes of klebsiella-reactive arthritis, usually in hla-b27-positive individuals. this concept is based on immunological, microbiological and serological considerations. immunological studies based on anti-b27 tissue typing sera and anti-b27 monoclonal antibodies indicate that hla-b27 cross-reacts with antigens found in klebsiella, salmonella, shigella and yersinia micro-organisms. salmonella, shigella and yersinia gut infections are ...19892670258
the yopm gene of yersinia pestis encodes a released protein having homology with the human platelet surface protein gpib alpha.in yersinia pestis kim, there are 11 yops (yersinial outer membrane proteins) encoded by the low-ca2+ response virulence plasmid pcd1. only yops m and n are found in easily detectable amounts in the culture medium. in this study, we located and characterized the yopm gene to obtain clues about its role in the virulence of y. pestis. rabbit antibody was raised against yops m and h, copurified from the supernatant of y. pseudotuberculosis 43(pgw600, pcd1 yope::mu di1[apr lac]). this antiserum was ...19892670888
francisella philomiragia comb. nov. (formerly yersinia philomiragia) and francisella tularensis biogroup novicida (formerly francisella novicida) associated with human disease.over a 12-year period, 16 human strains of a gram-negative, catalase-positive, halophilic, aerobic, nonmotile, small coccoid bacterium were received for identification. on the bases of biochemical characteristics and cellular fatty acid profiles, 14 of these strains were similar to the "philomiragia" bacterium (yersinia philomiragia, species incertae sedis). additional characteristics were growth on thayer-martin agar but no growth or sparse, delayed growth on macconkey agar; oxidase positive; a ...19892671019
pathogenic escherichia coli: a new etiology for acute ileitis in children.acute ileitis in children is frequently diagnosed at the time of laparotomy for acute abdominal pain and is usually due to inflammatory bowel disease, yersinia, or campylobacter infections. we report a case in which a pathogenic strain of escherichia coli was responsible for the disease. the diagnosis, microbiology, and epidemiology are discussed.19892671334
[clinical aspects, pathogenesis and treatment of yersinia infections]. 19892671487
[the first case of pseudotuberculosis found in china].this paper reports the first case of pseudotuberculosis in china. from the patient's stool, a strain of yersinia pseudotuberculosis was isolated. the isolate has all the classical characteristics of yersinia pseudotuberculosis and belongs to serotype iv.19892673528
ultrasound and ct of multifocal liver abscesses caused by yersinia enterocolitica.we present a patient with idiopathic liver hemochromatosis and mild secondary cirrhosis complicated by yersinia sepsis and miliary liver abscesses proven by echography and ct.19892674227
biological and toxic properties of deferoxamine. 19892675081
[conformational stability and immunochemical properties of yersinin--a basic protein of the outer membrane of the pseudotuberculosis microbe].using spectroscopic methods (circular dichroism and intrinsic protein fluorescence) and immunoenzyme assay, changes in the spatial and antigenic structure of yersinin, porin from outer membrane of yersinia pseudotuberculosis, were studied in solutions of ionic and non-ionic detergents at various temperatures and low ph values. yersinin was shown to retain its secondary structure under various denaturation conditions, the content of regular structural patterns depending on specific action of the ...19892675850
[antibacterial and bactericidal activities of japanese green tea].we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ...19892677434
[new data on the pathogenesis of reactive arthritis]. 19892680440
yersinia pseudotuberculosis infection as a cause of reactive arthritis as seen in a genitourinary clinic: case report.patients are often referred to the genitourinary clinic for screening for urethral infections after a clinical diagnosis of reiter's syndrome or reactive arthritis. we report a case of reactive polyarthritis in which serological evidence of yersinia pseudotuberculosis infection was found in the absence of the other common precipitating organisms, such as chlamydia spp, klebsiella spp, shigella spp, campylobacter spp, or yersinia enterocolitica.19892680895
[analysis of the plasmid composition of yersinia pseudotuberculosis strains and its use for typing pseudotuberculosis pathogens].electrophoresis in agarose gel has been used to study the plasmid spectra of 854 yersinia pseudotuberculosis strains isolated from different sources. the plasmids found in the microbial strains are represented by the elements with molecular masses 82; 57; 45; 5.5; 4.4; 3.5; 2.7; 2.4; 2.3 md. the variable spectra of plasmids is peculiar only for serovar i of yersinia pseudotuberculosis. plasmids p45 and p82 are classified as the main, while other plasmids as auxiliary ones. in accord with the cla ...19892682219
haemochromatosis and aldosterone deficiency presenting with yersinia pseudotuberculosis septicaemia.a 50 year old man presented with a pyrexial illness following a holiday abroad. yersinia pseudotuberculosis was isolated from blood culture. response to appropriate antibiotic therapy was prompt and complete, but full recovery was complicated by an episode of hyperkalaemia, hyponatraemia and the passing of large volumes of dilute urine. three years previously he had developed diabetes mellitus and hypogonadotrophic hypogonadism. investigation on recovery showed underlying haemochromatosis and al ...19892682586
[the differential diagnosis of salmonella abortus ovis and yersinia pseudotuberculosis in sheep abortions].55 strains of salmonella abortus ovis and 3 strains of yersinia pseudotuberculosis (two serotypes i, one serotype ii), isolated out of aborted ovine fetuses from northern bavaria, were investigated and compared culturally, biochemically and serologically. the identification of s. abortus ovis (group b) could not be achieved by use of api 20 e. the code-number 4004500/47 identified s. typhi (group d) only. y. pseudotuberculosis serotype ii but not serotype i agglutinated with the polyvalent and t ...19892683516
[the plasmid composition and pathogenetic characteristics of yersinia pseudotuberculosis strains isolated from human subjects against a background of epidemic and sporadic morbidity].112 newly isolated clinical cultures of y. pseudotuberculosis have been studied. the strains have been characterized by the presence of plasmids and pathogenicity signs associated with plasmids. the results of the study have confirmed the decisive role of the plasmid with a molecular weight of 44-48 md in the virulence of y. pseudotuberculosis. the plasmid with a molecular weight of 82 md, previously attributed the role of an epidemic marker, has also been found to be widely spread. our study ha ...19892683522
[findings of yersiniosis in the crimea]. 19892683523
[magnetic immunoenzyme analysis of yersinia pestis antigens].the detection of y. pestis cells in magnetic enzyme immunoassay is carried out with the use of magnetic polyacrylamide microgranules. in the assay system for the determination of the antigen commercial y. pestis antigens, peroxidase-labeled antibodies, the substrate mixture consisting of sodium salt of 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid and h2o2 in citrate-phosphate buffer solution, ph 4.5, are used. the sensitivity of the method is 5 x 10(4) microbial bodies per ml.19892683524
detection with synthetic oligonucleotide probes of nucleotide sequence variations in the genes encoding enterotoxins of escherichia coli.we examined variations in the genes encoding heat-stable enterotoxin (st) and heat-labile enterotoxin (lt) in 88 strains of escherichia coli isolated from individuals with traveler's diarrhea to find suitable sequences for use as oligonucleotide probes. four oligonucleotide probes of the gene encoding st of human origin (stib or sth), one oligonucleotide probe of the gene encoding st of porcine origin (stia or stp), and three oligonucleotide probes of the gene encoding lt of human origin (ltih) ...19892685027
spontaneous bacterial peritonitis due to yersinia enterocolitica in secondary alcoholic hemochromatosis.yersinia enterocolitica is a rare cause of systemic infections, including spontaneous bacterial peritonitis. most reported cases have been in patients with iron overload or host defenses compromised by chronic illnesses. we report the case of an alcoholic patient with hemochromatosis who developed spontaneous bacterial peritonitis due to yersinia enterocolitica.19892685097
[outbreaks of pseudotuberculosis and intestinal yersiniosis among soviet specialists and members of their families in the mongolian people's republic].in summer and winter of 1986 two outbreaks of alimentary enteric diseases occurred among soviet specialists and members of their families in ulan bator. these diseases were identified as yersinia infections registered in mongolia for the first time. in july 114 children in a kindergarten fell ill after being fed with salad prepared from vegetables and spring onions supplied from a nearby state farm. 20 y. pseudotuberculosis cultures, serovar 1, were isolated, and in 25 persons antibodies to the ...19892686302
clonal diversity and relationships among strains of yersinia enterocolitica.allelic variation in the chromosomal genome of 81 isolates of yersinia enterocolitica and single isolates of yersinia intermedia, yersinia frederiksenii, yersinia mollaretii, and yersinia kristensenii was assessed by analysis of electrophoretically demonstrable polymorphism in 21 genes encoding metabolic enzymes. eighteen distinctive multilocus genotypes (electrophoretic types [ets]) were identified. clustering of the ets from a matrix of pairwise genetic distances, based on the 21 enzyme loci, ...19892687316
cat-contaminated environmental substances lead to yersinia pseudotuberculosis infection in children.a 1-year-old boy was infected with yersinia pseudotuberculosis serotypes 1b and 3, and his 3-year-old brother was infected with y. pseudotuberculosis serotype 1b; both had drunk water from puddles in a garden of their housing district of miyoshi city, hiroshima prefecture, japan. the y. pseudotuberculosis serotype 1b and 3 strains isolated from soil from the dried-up puddles and sand and feces from the sandbox proved to be from a stray cat. the restriction endonuclease patterns of the plasmid in ...19892687319
[clinical aspects of yersinia pseudotuberculosis infections].during 2 years 27 cases of yersinia pseudotuberculosis infections were diagnosed in the infectious ward. on the basis of literary data and her own cases the author discusses in details the clinical aspects and varying forms of yersinia pseudotuberculosis infections. the attention is drawn to the fact that the pathogen is widespread, to the incidence of human infections and to the difficulties of differential diagnostics.19892689952
cloning and characterisation of the serc and aroa genes of yersinia enterocolitica, and construction of an aroa mutant.a gene library of yersinia enterocolitica 8081 was constructed in the cosmid vector phc79. recombinants containing the aroa gene, encoding 5-enolpyruvylshikimate 3-phosphate synthase, were identified by complementation of the aroa mutation in escherichia coli k-12 strain ab2829. all six recombinant plasmids which complemented aroa also complemented the serc mutation in e. coli k-12 strain kl282. tn5 mutagenesis suggested serc encoding 3-phosphoserine aminotransferase was the proximal gene in an ...19892691337
yersinia pseudotuberculosis enteritis in four calves.a yersinia pseudotuberculosis enteritis in four yearling calves is described. this is the first report of this organism causing enteric disease in domestic cattle in north america.19892691537
immunological and bacteriological aspects of reactive arthritis.it is apparent that in the development of reactive arthritis the patient fails in his first line of defence against the invading microorganism. this results in persistence of the microorganism probably in or close to the intestinal epithelium. microbial antigens may appear also in the circulation, perhaps as part of the immune complexes, or within cellular elements. through these transportation mechanisms they enter the synovium, triggering an inflammatory process that leads to reactive arthriti ...19892692127
mechanisms involved in the pathogenesis of yersinia infections.yersinia enterocolitica and yersinia pseudotuberculosis are food-borne enterobacterial pathogens which may initiate rheumatoid diseases. by molecular genetic analysis of the pathogenicity of these species virulence gene loci could be identified on the chromosome and on a plasmid. plasmid-encoded proteins mediate cell adherence, phagocytosis resistance, survival in serum, cytotoxicity, and collagen binding. y. enterocolitica of serotype 0:8 is mouse-lethal and arthritogenic for lewis rats. mouse ...19892692128
reactivity between monoclonal anti-hla-b27 antibodies and bacterial components: is there a consensus of findings?studies addressing the molecular aspects of the postulated cross-reactivity between the hla-b27 antigens and the bacteria known to precipitate arthritis (e.g., shigella, salmonella, yersinia and campylobacter) are reviewed. in conclusion, the necessity for further research into the pathogenesis of arthritis is stressed.19892692129
the role of proteae in diarrhea.a survey was undertaken on the occurrence of protease in the human fecal flora and its coincidence with other well-documented enteropathogens such as campylobacter, salmonella, shigella, staphylococcus aureus, yersinia, protozoa and rotavirus. a total of 2000 fecal specimens was investigated, 1000 from patients suffering from diarrhea and 1000 from healthy persons which served as controls. proteus mirabilis was isolated more frequently from diarrhea cases than from healthy people. the difference ...19892692584
incidence of sporadic infections due to enterotoxigenic escherichia coli (etec) in the population of hamburg, north germany.in two studies (1982 and 1985/86), the presence of enterotoxigenic e. coli (etec) and other enteropathogenic bacteria was investigated in 779 stool specimens from children and adults with and without diarrhoeal disease. etec was isolated from 2% of diseased children and 1.6% of adult patients, but also from 0.5% of asymptomatic persons. mixed infections with two different etec strains or with other enteropathogenic bacteria were identified in 4 of 9 infected persons. the etec strains belonged to ...19892692585
mammalian cell adhesion functions and cellular penetration of enteropathogenic yersinia species.the entry of enteropathogenic yersinia into cultured mammalian cells has been studied in order to gain insight into the mechanism of bacterial penetration into host cells during infection. there exist at least three pathways for entry by yersinia into mammalian cells, the most efficient of which is promoted by invasin, the product of the inv gene. invasin is an outer membrane protein that attaches to a mammalian cell receptor, initiating the entry process. several receptors that bind invasin hav ...19892693898
the yersinia yop regulon.growth of yersiniae is restricted at 37 degrees c in the absence of calcium ions. this phenomenon correlates with the massive release of a set of proteins called yops. growth restriction and yops production are governed by a 70 kb plasmid called pyv. yop genes are distributed throughout pyv and constitute a thermoactivated regulon controlled by the gene virf. the transcription activator virf is a member of a new family of regulators including those of the arabinose and rhamnose operons as well a ...19892693899
[various approaches to studying molecular-genetic mechanisms of low-temperature adaptation of microbial populations].the effect of biologically low temperature (12 degrees c) on the parameters of microbial population such as survival, catalase activity and its isoenzyme spectrum have been investigated on the models of escherichia coli and yersinia pseudotuberculosis. the quantity of nucleic acids, plasmid spectrum and temperature effect on the level of plasmid dna spiralization were studied under these conditions. the process of molecular genetic adaptation of bacterial populations having the broad temperature ...19892693957
isolation and properties of carboxylesterase p4 from yersinia pseudotuberculosis.the carboxylesterase p4 produced by yersinia pseudotuberculosis was purified 330-fold by gel permeation and deae-trisacryl chromatography with a final yield of 21%. the apparent molecular weight, as determined by fast-protein liquid chromatography, was 45 kda. the hydrolytic activity of esterase p4 was higher with the 1-naphthyl esters than with the 2-naphthyl esters of acetic, propionic and butyric acids. the apparent km values were identical for 1-naphthyl acetate and 1-naphthyl propionate (0. ...19892694247
[interleukin-1-inducing activity of the polysaccharide-containing antigens of the cell wall in yersinia pestis].the induction of the synthesis of interleukin-1 (il-1) in human monocytes under the influence of the endotoxic preparations (lps) and y. pestis basis somatic antigen has been experimentally studied. the results obtained in this study make it possible to come to the conclusion that the capacity of the endotoxin of y. pestis cell wall, consisting of lps of type r, for inducing the synthesis of il-1 in human monocytes is not different from the corresponding capacity of salmonella and shigella lps, ...19892694696
[acute diarrhea: recent etiopathogenetic findings].acute diarrhoeas in children represent still now a very serious problem for public health either in developing or in industrialized countries. in the last years great progress has been made in the aetiology and epidemiology of this disease. the most important results, which are worth recording, obtained in this field are: the detection of rotavirus as a more common agent causing infectious enteritis in children; the "rediscover" of some pathogenic agents (campylobacter and yersinia) ignored sinc ...19892698467
[the diagnostic value of immunoenzyme analysis in pseudotuberculosis]. 19892698536
[dynamic clinico-laboratory indices of physical and mental work capacity in pseudotuberculosis]. 19892698537
[prostaglandins and cyclic nucleotides in the gastrointestinal form of yersiniosis].in spite of the resemblance of the clinical picture of gastrointestinal yersiniosis and acute dysentery, material differences underlie the pathogenesis of these diseases. yersiniosis is marked by the predominance of an increase in the content of pgf2 alpha, whereas acute dysentery by an increase in the content of pge, which may be accounted for by greater intensity of the allergic manifestations in yersiniosis patients as compared with dysentery. shigellosis runs its course in the presence of th ...19892699095
[bacterial etiological factors of diarrhea in the stool of infants hospitalized in january 1987].the results are presented of the second part of the studies conducted for monitoring of the variability of bacterial factors causing diarrhoea in the youngest children. the presently reported study comprised 42 babies aged from 7 days to 18 months treated in hospital in january 1987. the number of children with diarrhoea hospitalized in this period was relatively smaller than in january 1985, while the incidence of enteropathogenic bacteria in stools was very similar. they included mainly klebsi ...19892701337
lcrh, a gene necessary for virulence of yersinia pestis and for the normal response of y. pestis to atp and calcium.we are investigating the functions of the three proteins encoded by the v operon (lcrgvh) of the low-calcium response virulence plasmid pcd1 of yersinia pestis kim5. the purpose of this study was to define the role of the 18-kilodalton protein encoded by lcrh, the third gene of the v operon. using marker exchange mutagenesis, we constructed a y. pestis mutant that failed to express the lcrh protein. this lcrh- mutant was "atp blind" in that it failed to show altered growth and v-antigen expressi ...19892707857
[prostaglandins and cyclic nucleotides in the dynamic development of an experimental poisoning syndrome caused by yersinia pestis lipopolysaccharide].this work deals with the influence of y. pestis lipopolysaccharide (lps), introduced intraperitoneally in a dose of 2 ld50, on the content of prostaglandins (pg), such as pge, pgf2 alpha and 6-keto-pgf1 alpha, thromboxane, camp and cgmp in the liver, lungs and blood plasma of guinea pigs in the process of the development of experimental intoxication. the content of thromboxane in blood plasma increased 2.4-fold in 2 hours after intoxication and remained elevated for as long as 5 hours. other par ...19892711790
structural variations and growth potential of yersinia enterocolitica under different culture conditions.the growth potential and the polypeptide composition of yersinia enterocolitica serotype 0:3 isolated from patients with uncomplicated diarrhoea, reactive arthritis or septicemia were evaluated under different culture conditions. the expression of polypeptides varied with presence of the virulence-associated 40-48 mdal plasmid, growth medium, growth temperature and gas composition of the culture (air, carbon dioxide, oxygen). also the initial growth medium at 26 degrees c, before temperature shi ...19892713134
comparison of bacteria with and without plasmid-encoded proteins as antigens for measurement of immunoglobulin m, g, and a antibodies to yersinia enterocolitica by enzyme-linked immunosorbent assay.yersinia enterocolitica serovar o:3 bacteria with and without plasmid-encoded proteins were compared as antigens in an enzyme-linked immunosorbent assay. good correlations between the two antigen preparations were obtained for immunoglobulin m (igm), igg, and iga antibodies of patients with yersiniosis. for routine diagnostic purposes, these antigens are considered equal.19892715330
occurrence of yersinia enterocolitica in the tokyo tama zoo.yersinia enterocolitica serogroups o5a and o8 were isolated from fecal samples of one colony of japanese macaques (macaca fuscata) from the tokyo tama zoo (japan). serogroup o5a was detected in brown bear (ursus arctos) prior to the isolation from the macaques. serogroup o5a organisms also were isolated from the japanese macaques' breeding area. serogroup o5a and o8 isolates were not pathogenic. serogroup o8 isolates did not possess the o7 or o19 antigens.19892716113
[the study of intra-articular infectious agents in reactive arthritis].reactive arthritis is differentiated from infectious arthritis by the lack of intraarticular infectious agents. recently 2 groups, using different techniques have demonstrated intra-articular antigens in cases of reactive arthritis associated with chlamydia and yersinia infections. in this article we report the preliminary results of screening cells from synovial fluid for dna of certain microorganisms by in situ hybridization. our findings provide complementary evidence of the intra-articular p ...19892718145
infectious complications due to transfusion acquired yersinia enterocolitica. 19892718240
[reiter's syndrome in patients with yersiniosis].reiter's syndrome is a rare pathology in patients with yersiniosis and occurs in 0.9% of all clinical forms of the disease. yersiniosis etiology was established in 7 patients with reiter's syndrome. the diagnosis was confirmed serologically. treatment consisted of levomycetin, gentamycin, in one patient--tetracycline as well as non-steroidal anti-inflammatory drugs indomethacin, metindol. all patients recovered completely within 1-2 months. no recurrences were noted within a 6-18 month follow-up ...19892718432
flaccid quadriparesis associated with yersinia enterocolitis-induced hypokalemia.a 23-year-old woman presented with diarrhea, flaccid quadriparesis, a low serum potassium level, and an elevated creatine kinase level. a stool culture yielded yersinia enterocolitica, and a muscle biopsy was compatible with a hypokalemic myopathy. the patient's diarrhea responded to sulfamethoxazole and trimethoprim therapy. we suggest that y enterocolitica be added to the group of intestinal pathogens capable of producing hypokalemia and rhabdomyolysis.19892719512
conservative management of a yersinia enterocolitica hepatic abscess.yersinia hepatic abscesses are rare, and are more likely to occur in patients with iron overload. a diabetic patient with haemochromatosis presented with a hepatic abscess due to yersinia enterocolitica, which was treated successfully by the use of a single antibiotic.19892719618
evaluation of dna colony hybridization and other techniques for detection of virulence in yersinia species.the virulence of yersiniae varies according to (i) species and biotype and (ii) possession of a 67- to 72-kilobase virulence plasmid. y. pestis, y. pseudotuberculosis, and biotypes 1b, 2, 3, 4, and 5 of y. enterocolitica are inherently virulent but express full virulence only when in possession of a virulence plasmid. other yersinia species and biotypes 1a and 3b of y. enterocolitica are seldom implicated in disease. in this study, we prepared dna probes from eight nonoverlapping regions of the ...19892723033
production of bacteriocin-like antagonism by clinical isolates of yersinia enterocolitica.fourteen clinical isolates of yersinia enterocolitica serotype o:3 and four well-documented virulent strains of serotypes o:3, o:8, and o:9 were biotyped and examined for plasmid-associated autoagglutination and calcium dependency and for epithelial cell adherence. these strains were tested for the production of bacteriocin-like antagonism by using tryptone soya blood agar at room temperature and at 37 degrees c. by using the cross-streaking method, three clinical isolates produced inhibitory su ...19892723036
suncus murinus. observations on ecology, distribution, status to plague in bombay.the common house shrew suncus murinus has been shown to play an important role in maintenance and perpetuation of plague infection by earlier plague workers. with the control of human plague there is no knowledge about foci of plague in small mammals associated with man. present study was carried out to fill in this lacuna. studies carried out in the present paper reveal that s. murinus does not harbour any plague infection in bombay. this species is widely distributed in bombay and is found to ...19892723422
fatty acid composition of lipopolysaccharides of the strains of different species of yersinia.the fatty acid composition of lipopolysaccharides of the strains of y. enterocolitica, y. intermedia, y. frederiksenii and y. ruckeri studied during cultivation on meat-peptone agar is characterized by the predominance of 3-hydroxytetradecanoic and dodecanoic acids. closely related to the mentioned bacteria is the strain of y. kristensenii which is distinguished only by its higher level of hexadecanoic acid. the strains of y. pseudotuberculosis and the vaccine strain of y. pestis have a uniform ...19892723424
[2 cases of parotitis in yersinia infection in adults]. 19892724879
[yersinia parotitis in children]. 19892726387
[entrapping of hydrophobized plague capsular antigen into large unilamellar liposomes].the entrapping of a plague capsular antigen into the liposomes, prepared by the "reverse phase" evaporating method, was investigated. both the native antigen and the antigen modified by palmitoylchloride were used. the entrapping of the modified antigen into the liposomes was greater than that of the native one: 46.1 +/- 5.4% and 3.7 +/- 2.7%, respectively. the highest content of the antigen on the membrane surface was observed when using palmitoylized protein and liposomes with egg lecithin, ch ...19892728118
[involvement of the eyes in yersiniosis].the results of clinical examinations of 15 patients with yersiniasis are analyzed. besides the involvement of the anterior segment of the eye, known before, the authors have detected for the first time 3 cases of the fundus oculi involvement; the impairments resemble multiplaquoid epitheliopathy. the clinical description is supplied with fluorescent angiograms. yersiniasis is regarded as a possible etiologic factor responsible for multiplaquoid epitheliopathy; this extends the scope of our knowl ...19892728169
[thymine- and thymidine-dependence of yersinia pestis mutants with altered thymidylate synthetase].thymine- and thymidine-dependent mutants of y. pestis strain ev-76 have been isolated and characterized. obtaining y. pestis thymine-dependent mutants in trimethoprim-containing media with full nutritional value in the presence of thymine and thymidine and the capacity of natural strains from the foci of infection in transcaucasia and mongolia to grow in such media indicate that y. pestis has gene tpp controlling thymidine phosphorylase, but this enzyme is strongly suppressed under normal condit ...19892728696
[yersinia pestis l-forms in rodents and ectoparasites].y. pestis l-forms and bacterial forms persist in the body of great gerbils for 40 days. l-forms are poorly phagocytized and can persist in phagocytes for a long time. in guinea pigs immunized with vaccine ev, y. pestis antigen could be detected till day 160. an unstable l-form was isolated from ornithodoros mites 3 years after their experimental infection with y. pestis. bacterial forms persist in mites for 1-3 years. for 5 years y. pestis antigen is regularly detected in a high percentage of mi ...19892728699
acute infective endocarditis due to yersinia enterocolitica. 19892729327
[study of trimethoprim sensitivity in strains of yersinia pestis isolated from various natural foci of plague].the effect of exogenic addition of purines and pyrimidines on trimethoprim sensitivity was studied in 134 vaccinal and virulent strains of the plague causative agent isolated at various periods in 12 natural plague foci. 74 strains proved to be sensitive to low concentrations of trimethoprim in the cultivation media irrespective of the presence or absence of thymine or thymidine in them. in this respect the strains differed from many other bacterial species which in the presence of thymidine or ...19892730213
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