| potential pathogens in the environment: cultural reactions and nucleic acid studies on klebsiella pneumoniae from clinical and environmental sources. | the phenotypic and nucleic acid properties of klebsiella pneumoniae have been studied on cultures obtained from six different habitats (humans, vegetables, seeds, trees, rivers, and pulp mills). the 19 cultural reactions of 107 isolates varied significantly only in tryptophanase activity and dulcitol fermentation. the percentage of guanine plus cytosine base composition of 41 isolates varied from 53.9 to 59.2%. the range of percentage of guanine plus cytosine base composition for environmental k ... | 1975 | 1098574 |
| membrane filter procedure for enumerating the component genera of the coliform group in seawater. | a facile, quantitative, membrane filter procedure (mc) for defining the distribution of coliform populations in seawater according to the component genera was developed. the procedure, which utilizes a series of in situ substrate tests to obviate the picking of colonies for identification, also provides an estimate of the total coliform density. when pure cultures of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae were suspended in seawater and held at 4 c for 24 h, between 56 ... | 1975 | 1098575 |
| nitrogenase of klebsiella pneumoniae. a stopped-flow study of magnesium-adenosine triphosphate-induce electron transfer between the compeonent proteins. | stopped-flow kinetic data have been obtained for a rapid electron-transfer reaction between the component proteins of nitrogenase from klebsiella pneumoniae, which was induced by mgatp. up to three equivalents of the fe-containing protein were rapidly oxidized by one equivalent of the fe-mo-containing protein in a unimolecular reaction, k2 = 2 x 10(2)s-1. evidence for a tight complex between the component proteins, kd(complex) less than 0.5 mum, which was formed with a rate k1 greater than 1 x 1 ... | 1975 | 1098654 |
| heat destruction of some bacterial strains attached to broiler skin. | 1. heat destruction of bacteria attached to the skin did not occur at a logarithmic rate provided the temperature was above 51 degrees c. 2. it was concluded that the bacteria are protected by their location in the skin surface rather than by polymers produced during attachment. 3. an analogous process is considered to take place during the scalding of broilers, since bacteria which survived were difficult to remove from the carcasses during further processing. | 1975 | 1098746 |
| in vitro and in vivo microbiological evaluation of cephapirin, a new antibiotic. | the microbiological properties of cephapirin, a new semisynthetis cephalosporin, have been studied. this antibiotic, if compared with ampicillin, shows a greater activity against gram positive bacteria, a lack of sensitivity to staphlococcal beta-lactamase, and a lower sensitivity to those produced by gram-negative bacteria. useful therapeutic of cephapirin levels can be detected in human serum 6 h after administration of 500 and 1,000 mg parenterally. | 1975 | 1098870 |
| association of gastroenteritis with urinary tract infection in infancy. | | 1975 | 1099002 |
| isolation of a hybrid f' factor-carrying escherichia coli lactose region and salmonella typhimurium histidine region, f42-400 (f' ts114 lac+, his+): its partial characterization and behavior in salmonella typhimurium. | episome f' ts114 lac+ (f42-114) was transferred into salmonella typhimurium carrying an f'his+ (fs400) episome, and fused episome f' ts114 lac+, his+ (f42-400) was obtained. episome f42-400 could be transferred to s. typhimurium, escherichia coli and klebsiella pneumoniae. identification of the episome was based on: (i) temperature sensitivity of the lac+ and his+ phenotypes; (ii) the fact that f- segregants, obtained after temperature curing or acridine orange curing, were simultaneously lac- a ... | 1975 | 1099076 |
| behavior of a hybrid f' ts114 lac+, his+ factor (f42-400) in klebsiella pneumoniae m5a1. | episome f' ts114 lac+, his+ (f42-400) was transferred from salmonella typhimurium to klebsiella pneumoniae. from the progeny, a strain of k. pneumoniae able to retransfer the episome was obtained. the his+ phenotype in this strain is temperature sensitive. escherichia coli female-specific phages phiii, w31, and t3 were shown to plate on k. pneumoniae. from phiii we obtained two derivatives; phiiik, which plates only on k. pneumoniae, and phiiie, which plates only on e. coli. growth of phages t3 ... | 1975 | 1099077 |
| escherichia coli capsule bacteriophages. vii. bacteriophage 29-host capsular polysaccharide interactions. | different interactions between particles of escherichia coli capsule bacteriophage 29 and its receptor, the e. coli serotype 29 capsular polysaccharide have been studied. the inactivation of phage 29 (8 x 10(3) pfu/ml) by isolated host capsular glycan was found to be physiologically insignificant (50% inactivation dose equals 100 mug after 1 h at 37 c). no adsorption (less than 2 x 10(4) pfu/mug) of the viruses to k29 polysaccharide-coated erythroyctes (at 0 or 37 c) was observed either. the pha ... | 1975 | 1099233 |
| microbial adjuvant and autoimmunity. i. induction of antibody responses to syngeneic tissue extracts in mice treated with capsular polysaccharide of klebsiella pneumoniae. | with the use of the capsular polysaccharide of klebsiella pneumoniae (cps-k) as a powerful adjuvant, high precipitin responses could be induced in mice to syngeneic eyeball extracts and thyroid gland extracts which were normally nonimmunogenic. only very weak responses were induced to eyeball extracts by freund's complete adjuvant. repeated administrations of the antigens mixed with cps-k at time intervals of 30 days (more than twice for the eyeballs or more than three times for the thyroid glan ... | 1975 | 1099286 |
| antibodies against enteric bacteria in brown bullhead catfish (ictalurus nebulosus, lesueur) inhabiting contaminated waters. | brown bullhead catfish were collected from sewage- and acid mine waste-polluted waters in an attempt to detect antibodies against human enteric bacteria in their sera and to investigate the association of antibody response with environmental conditions. agglutination antigens prepared from isolates obtained from water collected at the same locations as the fish habitats were used to demonstrate such antibodies. the results showed large percentages of reactive sera for common isolates such as esc ... | 1975 | 1099987 |
| comparison of leukocyte count and function in smoking and nonsmoking young men. | leukocyte function and other hematological measurements were tested in 14 smoking and 13 nonsmoking young men free of intercurrent or chronic diseases. leukocyte chemotaxis was depressed in smoking subjects when compared to the same subjects abstaining from cigarettes or to the nonsmokers. smoking did not affect the whole blood bactericidal capacity of leukocytes and serum for staphyloccus aureus or klebsiella pneumoniae. total leukocyte counts, hematocritis, and monocyte counts were higher in t ... | 1975 | 1100522 |
| klebsiella pneumoniae and staphylococcus aureus infection in mice: difference in uremia and ammoniagenesis. | lethal infections by staphylococcus aureus and klebsiella pneumoniae were compared for kidney-related effects in mice. k. pneumoniae caused uremia and an increase in blood ammonia that could reach 2.5 times normal. these events did not occur in mice inoculated with s. aureus. use of germfree animals indicated that most of the increase in ammonia arose from the gut, presumably due to greater availability of urea and ureolysis. injected ornithine restored blood ammonia to nearly normal levels and ... | 1975 | 1100523 |
| studies on sampling methods used in the control of hygiene in poultry processing. | | 1975 | 1100581 |
| resistance to trimethoprim in klebsiellae isolated before its introduction. | | 1975 | 1100591 |
| nature and properties of hexitol transport systems in escherichia coli. | in escherichia coli k-12 the naturally occurring hexitols d-mannitol, d-glucitol, and galactitol are taken up and phosphorylated via three distinct transport systems by a mechanism called either group translocation or vectorial phosphorylation. for every system, a membrane-bound enzyme ii-complex of the phosphoenolpyruvate-dependent phosphotransferase system has been found, each requiring phosphoenolpyruvate, enzyme i, and hpr or alternatively p-hpr as the phosphate donor. cells with a constitut ... | 1975 | 1100608 |
| antibody response of patients with malignancies to bacteremia with gram-negative bacteria. | antibody response, determined by means of the indirect bacterial hemagglutination tests, was studied in 58 consecutive patients with various malignancies whose blood culture yielded growth of enterobacteriaceae or pseudomonas and from whom serum specimens were obtained. of these patients 59% had a significant antibody response. the invading microorganisms were escherichia coli in 33 and klebsiella in 19 subjects, an antibody response being documented with essentially equal frequency (60 and 57% ... | 1975 | 1100659 |
| analysis of acetoin and diacetyl in bacterial culture supernatants by gas-liquid chromatography. | the acetoin and diacetyl contents of culture supernatants of voges-proskauer-positive "viridans" streptotocci, klebsiella pneumoniae and staphylococcus aureus, were determined by a gas liquid chromatographic procedure, in which supernatants were extracted with diethyl ether and diacetyl was measured on columns of 10% (wt/wt) polyethylene glycol 400 (peg 400) at 73 c. acetoin was converted to diacetyl, before analysis, by a simple oxidation procedure with ferric chloride and without a distillatio ... | 1975 | 1100672 |
| [clinical evaluation of diffusion of sodium cephalothin into blood stream following administration into pelvic dead space (author's transl)]. | | 1975 | 1100875 |
| sulfonamide resistance mechanism in escherichia coli: r plasmids can determine sulfonamide-resistant dihydropteroate synthases. | several natural isolate e. coli strains highly resistant to sulfonamides and antibiotics are shown to contain a sulfonamide-resistant dihydropteroate synthase (2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine-diphosphate:4-aminobenzoate 2-amino-4-hydroxydihydropteridine-6-methenyltransferase, ec 2.5.1.15) in addition to the normal sensitive enzyme. the resistant dihydropteroate synthases examined are determined by an r plasmid and are smaller and less heat stable than the normal sulfonamid ... | 1975 | 1101260 |
| bacteremia in a general hospital. a prospective study of 102 consecutive cases. | a prospective clinical-bacteriological study of 102 consecutive cases of confirmed bacteremia at a copenhagen city general hospital was carried out during 5 months of 1973 with special concern given to focus of infection and acquisition of microorganisms. valid positive cultures were obtained from 7.2 patients per 1000 admissions. 50 of the 102 bacteremias were by all probability acquired in the hospital, mainly due to transurethral manipulations or intravenous lines. pneumonia and hepatobiliary ... | 1975 | 1101372 |
| [protective effects of bacterial immunostimulants in mice infected by "klebsiella pneumoniae" resistant to antibiotics after mutation or by plasmid transfer]. | a streptomycin-resistant strain of k. pneumoniae obtained after mutation in vitro was found to be less virulent than the sensitive strain in mice. however, a single injection of lipopolysaccharides (lps) administered 24 hours before challenge increased the host's resistance to both strains. in contrast, the virulence was not changed in k. pneumoniae accepting r-factors for ampicillin, streptomycin, chloramphenicol, tetracycline and sulphonamide. the plasmids were transferred to k. pneumoniae fro ... | 1975 | 1101801 |
| regulation and genetics of bacterial nitrogen fixation. | | 1975 | 1101806 |
| effect of surfactants on antibiotic resistance. | the effectiveness of surfactants as potentiators of antibiotic activity on several resistant strains of bacteria, selected from clinical sources and laboratory collections, was studied using a tube dilution assay. bacterial strains included members of the enterobacteriaceae and staphylococci. cetyltrimethylammonium bromide (ctab), tween 80 (tw80), a mixture of n-alkyldimethyl betaines (l14), and alpha-(2,4,5-trichlorophenoxy) propionic acid (tcp) were tested in combination with pencillin g (peng ... | 1975 | 1101823 |
| purification and properties of the clotting enzyme from limulus lysate. | | 1975 | 1101893 |
| nitrogenases of klebsiella pneumoniae and azotobacter chroococum. complex formation between the component proteins. | 1. sedimentation velocity analyses of mixtures of highly purified component proteins of azotobacter chroococcum are consistent with the formation of a tight 1 : 1 complex in the absence of na2 s2 o4. 1 : 1 complex formation between complementary proteins from a. chroococcum and klebsiella pneumoniae was also observed. the addition of 5 mm na2 s2 o4 weakened the interaction between the a. chroococcum proteins and also the interaction between complementary proteins of a. chroococcum and k. pneumon ... | 1975 | 1101961 |
| potent combinations of beta-lactam antibiotics using the beta-lactamase inhibition principle. | several penicillins known to be stable to enterobacterial beta-lactamases were tested in combination with beta-lactamase-sensitive penicillins and cephalosporins in a turbidimetric system. nafcillin was found to be the best beta-lactamase inhibitor amongst agents presently available commercially, but the related, new semi-synthetic penicillin brl 1437 (2-isopropoxy-1-naphthylpenicillin) was consistently found to be superior. using 103 ampicillin-resistant coliform bacilli and antibiotic levels a ... | 1975 | 1102266 |
| nitrogenase in azotobacter chroococcum and klebsiella pneumoniae. | | 1975 | 1102367 |
| properties of the r factor r144drd3 in klebsiella pneumoniae strain m5al. | | 1975 | 1102393 |
| dosage adjustments of cefazolin according to the pharmacokinetics of this new cephalosporin. | serum and urine levels of cefazolin were studied: 1. following a single 500mg intra-muscular dose of the drug in normal subjects (n = 10), in patients with renal impairment (n = 12) and in patients undergoing maintenance hemodialysis (n = 10); 2. following a single 500 mg intra-venous dose of the drug in normal subjects (n = 10). in normal subjects, serum half-lives averaged 1.75 hour after intravenous injection (ke = 0.395) and 2.22 hours after intra-muscular injection (ke = 0.312); urinary rec ... | 1975 | 1102452 |
| effect of microbial substances of different origins on the growth of mycobacterium leprae. | mycobacterium leprae multiplied in media enriched with substances originating from other mycobacteria, from non-acid-fast actinomycetales or from gram-positive or gram-negative eubacteriales. most of the m. leprae strains did not grow on a synthetic medium containing the amino acids present in m. smegmatis, but the growth-promoting effect of sonic extracts of this organism indicated that substances of bacterial origin, other than amino acids, do act as growth factors. the identification of the g ... | 1975 | 1102491 |
| incorporation of pantothenate into citrate lyase by a pantothenateless mutant of klebsiella pneumoniae. | a pantothenate-requiring mutant of klebsiella pneumoniae was isolated. the mutant showed an absolute dependence on pantothenate for growth. when grown in the presence of [14c]pantothenate, the mutant incorporated [14c]pantothenate into citrate lyase (3.4 mol/mol of enzyme). analysis of a double-labeled enzyme ([14c]pantothenate and [3h]acetate) by gel electrophoresis in sodium dodecyl sulfate showed that both 3h and 14c were associated solely with the smallest subunit, the acyl carrier protein o ... | 1975 | 1102528 |
| aminoglycoside therapy of gram-negative bacillary meningitis. | the distribution of aminoglycosides in the cerebrospinal fluid (csf) space was examined after intralumbar, intraventricular, and systemic administration during seven episodes of gram-negative bacillary meningitis. six episodes were associated with culture proved ventriculitis. parenteral therapy with gentamicin or tobramycin produced low concentrations of aminoglycoside (less than 1.0 mug/ml) in the lumbar, ventricular, and cisternal csf. administration of 5 to 10 mg of aminoglycoside into the ... | 1975 | 1102982 |
| antibiotic resistnace and r factors in klebsiella pneumoniae. | strains of klebsiella pneumoniae isolated from sewage were examined for their resistance spectra and for the presence of r factors. infectious drug resistance was demonstrated in 104 (80%) of the 130 strains tested. resistance to chloramphenicol, kanamycin or tetracycline was usually r factor-determined. resistance to gentamicin was not encountered in this species. | 1975 | 1103329 |
| bacteremia arising from the urinary tract. | | 1975 | 1103413 |
| [isolation of l-forms of klebsiella from patients with scleroma]. | | 1975 | 1103512 |
| bacterial flora in relation to cataract extraction. iii. postoperative flora. | the conjunctival flora of 499 patients operated for cataract was studied on the 4th and 7th postoperative days and compared with the flora examined previously on admission to the hospital and at the time of surgery (fahmy et al. 1975b, c). antibiotics had been administered approximately 18 hours before operation, at the conclusion of surgery, and then on the 4th postoperative day after the cultures had been taken. after a significant fall in incidence, including all kinds of bacteria at the time ... | 1975 | 1103562 |
| gram-negative bacillary meningitis. | all cases of unusual types of gram-negative bacillary meningitis in a university hospital over a five year period were retrospectively analyzed. these patients comprised 4.2 per cent of cases of bacterial meningitis among all patients, 69 per cent of neurosurgical cases and 42 per cent of neonatal cases. the over-all mortality was 40.3 per cent. the two most common bacterial isolates were escherichia coli in patients younger than one year and klebsiella species in patients above that age. infect ... | 1975 | 1103620 |
| effects of an antibacterial soap on the ecology of aerobic bacterial flora of human skin. | the effects of ad lib use of an antibacterial soap containing 1.0% trichlorocarbanilide and 0.5% trifluoromethyldichlorocarbanilide on the bacterial flora of six skin sites of 132 subjects were measured by comparison with the flora of 93 control subjects who avoided the use of topical antibacterials. each subject was examined once. the test soap produced significant reductions in geometric mean counts of the total aerobic flora on the back, chest, forearm, calf, and foot; counts were also reduce ... | 1975 | 1103729 |
| [turbidimetric microbiological semiautomatic determination of erythromycin]. | | 1975 | 1103905 |
| [breeding hygienic studies in horse breeding in the rhinevalley-palatinate during the breeding period 1973 and 1974]. | | 1975 | 1104321 |
| acetylene reduction by klebsiella neumoniae in air related to colony dimorphism on low fixed nitrogen. | | 1975 | 1104769 |
| [studies on the disc method for the determination of bacterial sensitivity to sulfobenzylpenicillin (author's transl)]. | the mic''s of sulfobenzylpenicillin (sb-pc) were determined by the two-fold serial agar-dilution method for 108 bacterial strains of 21 species. the diameters of inhibition zones of these bacterial strains by the 30 mug and 200 mug sb-pc discs were also measured. the relation between the mic and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about ... | 1975 | 1104912 |
| [in vitro examination on antibacterial activities of broad-spectrum antibiotics against gram-negative clinical isolates (author's transl)]. | in vitro studies were undertaken on clinically isolated strains of various bacteria to examine the antibacterial activity of ampicillin (ab-pc), carbenicillin (cb-pc), sulbenicillin (sb-pc) cephalothin (cet), cefazolin (cez) and gentamicin (gm). fifty three % of escherichia coli strains 53.3% of citrobacter sp., 80% of proteus mirabilis, 42.9% of morganella and 80% of bacteroides sp. were susceptible (mic being not higher than 12.5 mug/ml) to ab-pc. the eight other species were not so susceptibl ... | 1975 | 1104913 |
| [adjuvant action of capsular polysaccharide of klebsiella pneumoniae on antibody response--the role of adjuvant in initiation and amplification of the antibody-forming system]. | | 1975 | 1104919 |
| [delayed hypersensitivity reaction to capsular polysaccharides of klebsiella pneumonia]. | | 1975 | 1104920 |
| adjuvant action of capsular polysaccharide of klebsiella pneumoniae on antibody response. iv. the roles of antigen and adjuvant for induction of primary and secondary antibody responses and for development of immunological memory to bovine serum albumin. | a study was performed to clarify the roles of primary and secondary injections of antigen and adjuvant (capsular polysaccharide of klebsiella pneumoniae, cps-k) in induction of antibody responses and in the development of immunological memory in mice to bovine serum albumin (bsa). a primary injection of bsa alone neither induced significant primary antibody response nor increased immunological memory for a secondary antibody response but, if primary injections of bsa and cps-k were performed sim ... | 1975 | 1104933 |
| r-factors in urinary tract infection. | | 1975 | 1104976 |
| vesico-ureteric reflux and renal scarring. | | 1975 | 1104981 |
| kanamycin and gentamicin treatment of neonatal sepsis and meningitis. | mortality from neonatal meningitis due to gram-negative microorganisms remains 50% despite use of aminoglycoside antibiotics. blood was obtained on 238 occasions from 77 neonates with putative or documented sepsis; paired blood and cerebrospinal fluid (csf) samples were obtained on 14 occasions from ten neonates with meningitis. kanamycin and gentamicin were measured by a radioisotopic assay procedure. kanamycin was administered at 15 mg/kg/day in three divided doses intravenously; serum concent ... | 1975 | 1105375 |
| investigations on the efficacy of surface disinfection and surface cleaning procedures. 2. laboratory testing of the efficacy under conditions simulating those of real-life. | using a standardised method (impression method by means of 'rodac' plates and glass as well as ceramic surfaces as germ carriers) we tested for the efficacy of 3 surface disinfectants (aldehydes, aldehydes + detergent substances, phenol derivatives) and 3 disinfectant cleaning agents (aldehydes + detergent substances + wax) and 2 per cent soft soap solution on staphylococcus aureus, klebsiella pneumoniae and sarcina lutea. the method enabled us to calculate the actual germ count reduction and al ... | 1975 | 1106069 |
| investigations on the efficacy of surface disinfection and surface cleaning procedures. 3. evaluation of the results of in-use and laboratory tests. | 3 disinfectants and 3 disinfectant cleaning agents were subjected to comparative tests as to their germicidal activity. these were (a) laboratory tests with germ carriers and (b) in-use tests with sarcina-contaminated floor surfaces in a medical institute. the results, details of which are given in the two preceding publications, were assessed under the following angles: validity of the test methods, requisite changes of the test procedures and practical usefulness of the different types of prep ... | 1975 | 1106070 |
| [some problems of nomenclature and taxonomy of the enterobacteriaceae]. | | 1975 | 1106093 |
| the enzymology of the formation and breakdown of citrate. | | 1975 | 1106128 |
| evaluation of coli-count samplers for possible use in standard couting of total and fecal coliforms in recreational waters. | millipore coli-count samplers were used to enumerate colonies of laboratory cultureunts than standard membrane0filter procedures for both total and fecal coliforms. althought the samplers are useful for semiquantitative analysis as indicated by the manufacturer, they are not suitable examinations of recreational waters. | 1975 | 1106326 |
| hyperbilirubinaemia and bacterial infection in the newborn. a prospective study. | the incidence of bacterial infection associated with unexplained hyperbilirubinaemia was determined prospectively in 69 infants under 2 weeks of age. blood and urine cultures were obtained from all patients, and 22 patients had their csf cultured. bacterial infection was documented in only 2 infants, who had asymptomatic gram-negative urinary tract infection. | 1975 | 1106333 |
| production of gluconic acid and 2-ketogluconic acid by klebsiella aerogenes ncta 418. | 2-ketogluconic acid and, to a lesser extent, gluconic acid were found to be major products of glucose catabolism by phosphate-limited cultures of klebsiella aerogenes nctc 418, and together accounted for up to 46% of the glucose carbon that was metabolized. although the concentrations of both acids increased substantially at low growth rates, their specific rates of synthesis decreased markedly, ad did the proportion of glucose converted into these products. determination of the affinity constan ... | 1975 | 1106345 |
| search for bacterial l forms in some diseases of human pathology. | the authors report their investigations performed in the attempt to isolate bacterial l forms or protoplasts from human materials (urine, blood) and relate their presence with an underlying pathology, and discuss the results obtained. | 1975 | 1106727 |
| maintenance and amplification of cell-associated immunological memory in in vivo culture system. | | 1975 | 1106873 |
| extrachromosomal deoxyribonucleic acid in r factor-harboring enterobacteriaceae. | extrachromosomal deoxyribonucleic acid (dna) from 24 different r factor-harboring enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. the r factors represented 15 different patterns of transferable drug resistance found in enterobacteria from an enclosed geographic area. all of the strains contained extrachromosomal, circular dna molecules within the range of 0.4 to 52 mum. more than one size class of circular dna molecules was observed in ... | 1976 | 1107322 |
| construction of intergeneric hybrids using bacteriophage p1cm: transfer of the klebsiella aerogenes ribitol dehydrogenase gene to escherichia coli. | study of many of the interesting properties of klebsiella aerogenes is limited by the lack of a well-characterized genetic system for this organism. our investigations of the evolution of the enzyme ribitol dehydrogenase (ec 1.1.1.56) in k. aerogenes would be greatly facilitated by the availability of such a system, and we here report two approaches to developing one. we have isolated mutants sensitive to the coliphage p1, which will efficiently tranduce genetic markers between such sensitive st ... | 1976 | 1107331 |
| control of microflora in stallion semen with a semen extender. | sterile equipment was used to collect ten ejaculates from each of ten normal stallions and quantitative and qualitative bacterial counts were made within 15 min after collection. the mean bacterial population in undiluted semen was found to be 573,000 +/- 374,000 organisms/ml. the bacterial content of semen diluted with two parts sterile physiological saline, or with two parts of a cream-gelatin extender, was measured within 15 min after collection and again after 2 hr at 25 degrees c. the numbe ... | 1975 | 1107540 |
| editorial: iatrogenic nonsuppurative infected thrombophlebitis. | | 1976 | 1107609 |
| antenatal infection: adequate protection against hyaline membrane disease? | it has been argued that fetal and placental infections decrease the incidence of hyaline membrane disease (hmd). however, others contend that this is not so. we performed a rigidly controlled clinicopathologic investigation of one group of infants with evidence of severe antenatal infection compared with another group free of infection. this study shows that placental infection correlated positively with neonatal sepsis and that in this series of patients neither infection nor prolonged rupture ... | 1976 | 1107908 |
| [klebsiella-pneumonia in low-birth-weight newborn infants. cumulative incidence in a premature department]. | | 1976 | 1107937 |
| evaluation of amniotic fluid for aerobic and anaerobic bacteria. | studies of 117 pregnant women, 83 at term, were instituted to determine the bacteriologic state of amniotic fluid, utilizing both standard aerobic and anaerobic technics. a high association of postpartum infection was found in women who had long periods of premature reptured membranes and many vaginal examinations. significant organisms including anaerobes, were isolated in many of these instances. based on the findings of these studies, it is recommended that amniocentesis for aerobic and anaer ... | 1976 | 1108641 |
| amikacin therapy of infections in neutropenic patients. | amikacin, a new aminoglycoside antibiotic, was utilized in the treatment of 49 cases of infection which occurred in 39 neutropenic cancer patients. thirty-four patients (69 per cent) responded to this antibiotic. pneumonia and septicemia were the most common types of infection treated and the response rates were 65 per cent and 75 per cent, respectively. gram-negative bacili were responsible for 93 per cent of the identified infections and 74 per cent responded. e. coli, ps. aeruginosa, and orga ... | 1975 | 1108649 |
| [study of the transferable factors of antibiotic resistance in the enterobacteria isolated at the regional hospital center of nantes]. | characteristics of transferable resistance of 72 entero-bacteriaceae resistant to one more antibiotic, isolated from february to september 1972, showed that this was common. multi-resistant strains permit one to obtain transfer of various characteristics either alone or together in 76 per cent of cases, whereas strains with less than three characteristics, transferred in only 9 per cent of cases. the transfers were uncommon in the case of proteus compared with other enterobacteriaceae and, in pa ... | 1975 | 1108714 |
| treatment of respiratory klebsiella pneumoniae infection in mice with aerosols of kanamycin. | aerosols of kanamycin resulted in greater survival of mice challenged with respiratory klebsiella pneumoniae than the same dosage given intramuscularly. determinations of viable bacteria in the blood and lungs revealed that aerosolized kanamycin was most effective when infection was confined to the lungs. after the organisms had spread to other areas, however, aerosol therapy was still more effective than intramuscular therapy, but only one-half the infected mice survived. | 1975 | 1108778 |
| isolation of galantins i and ii, water-soluble basic peptides. studies on antibiotics from the genus bacillus. iii. | two water-soluble basic antibiotics named galantins i and ii were isolated from a strain resembling bacillus pulvifaciens. both antibiotics are peptides containing glycine, alanine, ornithine, lysine and some unknown ninhydrin-positive components. an approximate empirical formula c50 plus and minus h98plus and minus 2o17n16 is indicated for galantin i. these are active against some gram-positive, acid-fast and gram-negative bacteria. | 1975 | 1112763 |
| citrate uptake in membrane vesicles of klebsiella aerogenes. | in whole cells of klebsiella aerogenes grown anaerobically on citrate as sole carbon source, citrate uptake is followed by rapid catabolism of the substrate via the inducible citrate fermentation pathway. membrane vesicles prepared from such cells take up citrate but do not catabolize it. vesicles process d-lactate dehydrogenase and the na+-requiring oxalacetate decarboxylase. citrate is taken up in the presence of na+, and other monovalent cations, such as nh4+, rb+, cs+, or k+, do not substitu ... | 1975 | 1112775 |
| a maximal predictive classification of klebsielleae and of the yeasts. | the concepts of the numerical method of maximal predictive classification are illustrated with classifications of 13 species of enterobacteria and of 434 species of yeast. the method seeks to classify into a specified number of classes (k) such that more correct statements can be made about the constituent members than with any other classification. the best choice of k relates to the separation of the classes as measured by the average number of correct statements made for an individual assigne ... | 1975 | 1113078 |
| [recent studies on the in vivo and in vitro activity of gentamicin]. | the effect of gentamycin was investigated at the university of modena phthisiology and respiratory diseases department. in vitro activity against most pathological gram-positive and gram-negative bacteria was observed. a dose of 4-5 mg/kg proved very effective in cases of bacteria-induced bronchopneumopathy. the drug was also active against penicillin-resistant staphylococci and gram-negative bacteria with a known resistance to ordinary antibiotics. | 1975 | 1113913 |
| fragments of klebsiella bacteriophage no. 11. | | 1975 | 1114709 |
| citrate lyase from streptococcus diacetilactis. association with its acetylating enzyme. | citrate lyase (ec 4.1.3.6) was purified 38-fold from cell-free extracts of streptococcus diacetilactis. the enzyme was homogeneous in analytical ultracentrifugation and polyacrylamide gel electrophoresis. the final enzyme preparation contained acetate: hs-citrate lyase ligase--an acetylating enzyme which converts inactive hs-citrate lyase into enzymatically active acetyl-s-citrate lyase. this enzyme activity was purified 25-fold over the crude extract and seemed to be associated with citrate lya ... | 1975 | 1115558 |
| catecholamine and phagocytic responses in infected mice exposed to hyperbaric helium-oxygen atmospheres. | exposure of mice to exotic gaseous environments (97% helium and 3% oxygen) maintained at 20% cand 8 ata (100 psig) increased host susceptibility to aerosol infection with klebsiella pneumoniae. the animals became ill earlier than the controls and the respiratory ld50 for the hyperbaric mice was about 50% smaller than for controls under ambient conditions. furthermore, hyperbaricity induced an acute depression in phagocytic activity and a marked elevation in the circulating levels of epinephrine, ... | 1975 | 1115719 |
| regulation of arylsulfatase synthesis by sulfur compounds in klebsiella aerogenes. | in klebsiella aerogenes, arylsulfatase synthesis was repressed by inorganic sulfate, sulfite, sulfide, thiosulfate, and cysteine, but not by methionine under normal growth conditions. we isolated cysteine-requiring mutants (cys minus), and mutants (atss minus, atsr minus) in which the regulation of arylsulfatase synthesis was altered. in the cysteine auxotroph, enzyme synthesis was also repressed by inorganic sulfate or cysteine. kinetic studies on mutants of the cysteine auxotroph showed that i ... | 1975 | 1116990 |
| a transvesical approach to total seminal vesiculectomy: a case report. | | 1975 | 1117507 |
| transbronchial catheterization of pulmonary abscesses. | a major indication for bronchoscopy in patients with pulmonary abscess is the need to establish adequate drainage. often, associated bronchial swelling precludes direct entry into the cavity with standard suctioning devices. satisfactory drainage may require numerous bronchoscopic procedures, prolonging hospitalization and subjecting the patient to the complications of pyogenic pulmonary abscess. we have recently adopted the technique of passing angiography catheters through the rigid bronchosco ... | 1975 | 1119880 |
| changes in cutaneous flora after wet occlusion. | aerobic flora from wet-occluded forearms of six volunteers was sampled the day before treatment, on the 3rd day when dressings were removed, and daily, when possible, for 8 days thereafter. erythema was not present. all bacterial colonies appearing on appropriate dilution plates were identified with the aid of a replica-plating technique. flora of each individual increased to over 10-4 colony-forming units/cm2 as a result of wet-occlusion, but counts rapidly fell by about 10-2 units once dressin ... | 1975 | 1122426 |
| on the molecular weights of the three nonidentical subunits of citrate lyase from klebsiella aerogenes. | the molecular weights of the three nonidentical subunits of citrate lyase of klebsiella aerogenes have been determined by three methods: sedimentation equilibrium in 6 m guanidinium chloride, sodium dodecyl sulfate gel electrophoresis, and gel filtration on 6 percent agarose column in 6 m guanidinium chloride. the molecular weights of the subunits, names i, ii, and iii (or acyl carrier protein) in order of elution from the agarose column, were 54,500, 32,000, and 11,000, respectively. the agaros ... | 1975 | 1123340 |
| the effect of operating-room environment on the infection rate after charnley low-friction total hip replacement. | after 300 charnley low-friction arthroplasties done in two hospitals by two surgical teams using clean-air enclosures but no preoperative, operative, or postoperative antibiotics, there were three deep wound infections, two caused by staphylococcus albus, coagulase negative, and one by klebsiella. two of these infections occurred in hips previously operated on and one was in a hip with no previous surgery. these findings suggest that two operating-room environments using the same basic principle ... | 1975 | 1123376 |
| the flora of renal haemodialysis shunt sites. | during investigations of the microbial flora of the skin over haemodialysis shunt sites it has not proved possible to predict clinical infection by a preceding colonization of the shunt site with a pathogenic organism. the normal non-pathogenic flora of the sites is not specifically related to the flora of other sites on the body though staphylococcus aureus on a shunt site appeared to be acquired principally from the nose when the shunt was in the arm or from the perineum when the shunt was in ... | 1975 | 1123436 |
| letter: "impossible" serum gentamicin levels. | | 1975 | 1125682 |
| letter: massive pulmonary gangrene. | | 1975 | 1125903 |
| acute thyroiditis with gas formation. | | 1975 | 1127327 |
| experience with detailed surveillance of nosocomial infection. | infection rates have been determined in a 332 bed university hospital using an efficient surveillance program. this computerized program analyzed, summarized and returned infection reports in six days. the efficiency rate of this system was 80 per cent as determined by prevalence survey. the mean nosocomial infection rate for the 12 month study period was 10.8 per cent. neurology, medicine, neurosurgery, surgery and urology ahd the highest attack rates. urinary tract infections composed 44 per c ... | 1975 | 1129686 |
| susceptibility of clinical isolates of enterobacteriaceae to bl-s640, a new oral cephalosporin. | the in vitro activity of bl-s640, a 7-(2-aryl-2-aminoacetamido)-3-(heterocyclic-thiomethyl) cephalosporin, was evaluated against 338 clinical isolates of enterobacteriaceae in comparison with ampicillin, cephalothin, cefazolin, and cephalexin. against escherichia coli, bl-s640 was as active as cefazolin and more active than ampicillin, cephalothin, and cephalexin. bl-s640 was as effective as the other cephalosporins tested and far more active than ampicillin against klebsiella and was more activ ... | 1975 | 1137392 |
| trimethoprim-sulfamethoxazole in chronic bronchitis. | authenic tracheobronchial secretions/exudates (tbse) were aspirated under direct vision via a sterile catheter passed through a fiberoptic bronchoscope from patients with chronic obstructive pulmonary disease complicated by chronic bronchitis. tbse, saliva and blood were obtained during long-term administration of trimethoprim-sulfamethoxazole (tmp-smx) and were assayed for drug content. before and during treatment tbse were cultured qualitatively and quantitatively for aerobic and anaerobic bac ... | 1975 | 1137833 |
| modification of polysaccharides containing uronic acid residues. | klebsiella type 47 capsular polysaccharide has side chains attached to the main chain via d-glucuronic acid residues. the side chains have been removed to yield an essentially linear polysaccharide by the following sequence of reactions: (1) substitution of hydroxyl and carboxyl groups with methyl vinyl ether; (2) beta-elimination by treatment with base; (3) removal of modified uronic acid residues and protecting groups by mild acid hydrolysis. the possibility of modifying other uronic acid-cont ... | 1975 | 1137839 |
| induction by sodium of the citrate fermentation enzymes in klebsiella aerogenes. | | 1975 | 1140396 |
| [constrictive pericarditis in infants]. | | 1975 | 1143967 |
| faecal stasis and bacteriuria: experimental research in rats. | | 1975 | 1145908 |
| nodular subcutaneous fat necrosis. a manifestation of silent pancreatitis. | a 57-year-old man with asymptomatic pancreatitis developed tender subcutaneous nodules with fat necrosis. these nodules were the sole clinical manifestation of the underlying disease. this case demonstrates that silent pancreatitis should be considered in the differential diagnosis of painful leg nodules. | 1975 | 1147633 |
| introduction: complement diseases in man. | | 1975 | 1148384 |
| massive empyema due to citrobacter diversus. | citrobacter diversus is a gram-negative rod member of the enterobacteriacease family. a patient is described from whom this organism was isolated twice in pure culture from empyema fluid. our isolates of citrobacter diversus were resistant to ampicillin and carbenicillin and sensitive to cephalothin. citrobacter diversus should be distinguished from citrobacter freundii, enterobacter cloacae and klebsiella pneumoniae. this organism joins streptococcus pyogenes, bacteroids species, anaerobic stre ... | 1975 | 1149508 |
| bacteremia in febrile children under 2 years of age: results of cultures of blood of 600 consecutive febrile children seen in a "walk-in" clinic. | | 1975 | 1151561 |
| dual pathways of glycerol assimilation in klebsiella aerogenes ncib418: their regulation and possible functional significance. | klebsiella aerogenes ncib 418 assimilates glycerol via alternative pathways: one involves a glycerol kinase with a high affinity for glycerol (apparent km = 1-2x10(-6)m), and the second a glycerol dehydrogenase with a much lower affinity for its substrate (apparent km=2-4x10(-2)m). in variously-limited chemostat cultures, one or the other pathway predominated. thus, aerobic carbon-limited organisms contained only the glycerol kinase pathway whereas aerobic sulphate-limited or ammonia-limited o ... | 1975 | 1156097 |
| [occurances of microbes of the family enterobacteriaceae in drugs. ii. findings and properties of the genera enterobacter and klebsiella]. | | 1975 | 1157106 |
| fosfomycin in treatment of chronic urinary tract infection. | a study was made of fosfomycin in the treatment of chronic urinary infection in 51 patients. 72% of the 67 bacteria isolated and tested in the laboratory with discs containing 50 mug of fosfomycin were found to be sensitive to the antibiotic. the majority of e. coli, klebsiella, p. mirabilis and ps. aeruginosa strains were sensitive to the antibiotic. generally, 60 mg of fosfomycin/kgbody weight was administered intramuscularly 6-hourly for 7-15 days. a clinical and bacteriological cure was obta ... | 1975 | 1157571 |