| [bacteremia caused by campylobacter jejuni resistant to nalidixic acid and fluoroquinolones]. | | 1993 | 8461378 |
| evaluation of commercial antisera for serotyping heat-labile antigens of campylobacter jejuni and campylobacter coli. | commercial antisera for serotyping 22 heat-labile antigens of campylobacter jejuni and campylobacter coli were evaluated by using 66 isolates from human and nonhuman sources. test results were compared with results of tests using antisera produced at the centers for disease control (cdc), atlanta, ga. all strains (three isolates of each of the 22 serotypes) were typeable with the cdc antisera. of 66 test strains, 39 (59%) were typed as the same serotype with both sets of antisera. twenty-four st ... | 1993 | 8463402 |
| increased resistance to quinolone in catalonia, spain. | from 1989 to 1991, the level of resistance to ciprofloxacin in our hospital increased from 0.47% to 6.7% in opportunistic enterobacteriaceae, from 9.9% to 16% in pseudomonas aeruginosa and from 8.27% to 31.8% in campylobacter jejuni-coli. we also observed an increase in quinolone consumption from 1.1 in 1989 to 1.5 defined daily doses per 1000 inhabitants per day in 1991. | 1993 | 8467626 |
| a point source outbreak of campylobacter infection related to bird-pecked milk. | a point source outbreak of campylobacter jejuni affected 11 children in a day nursery. milk consumed by the children was known to have been pecked by magpies on occasions. illness was significantly associated with consumption of milk on a single morning. examination of milk from a bottle pecked after the outbreak yielded campylobacters. the level of contamination was approximately six cells of c. jejuni per 500 ml of milk. | 1993 | 8472769 |
| the role of management systems in the epidemiology of thermophilic campylobacters among poultry in eastern zone of tanzania. | a total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using skirrow's protocol. the organisms were isolated from 90 (35.3%) of all samples. among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. significantly large numbers of isolations, 68 of 14 ... | 1993 | 8472771 |
| colonization of broiler chickens by waterborne campylobacter jejuni. | chickens on a broiler farm in southern england were found to be colonized with campylobacter jejuni of a single serotype, lior 1 penner 4. the farm was the sole supplier of a local slaughterhouse associated with a campylobacter outbreak in 1984 caused by this serotype. the serotype persisted on the farm for at least 18 months after the outbreak; its prevalence in the human population served by the farm remained high until it disappeared from the farm in 1986. the possible sources and routes of t ... | 1993 | 8476300 |
| arcobacter (campylobacter) butzleri-associated diarrheal illness in a nonhuman primate population. | after dna hybridization identified an isolate from an ill rhesus macaque (macaca mulatta) as arcobacter (campylobacter) butzleri, we initiated a study to determine whether a. butzleri was associated with diarrheal disease in nonhuman primates at the yerkes primate research center. by using campy-cva medium incubated at 35 degrees c, 15 a. butzleri isolates were obtained from 14 macaques; 7 macaques were coinfected with campylobacter coli and campylobacter jejuni. a. butzleri was not isolated fro ... | 1993 | 8478115 |
| in-vitro activity of dirithromycin in comparison with other new and established macrolides. | improvements with regard to the in-vitro activity of new macrolides are marginal and apply mainly to haemophilus spp., moraxella catarrhalis and neisseria gonorrhoeae (e.g. azithromycin is two to eight times more active than erythromycin) and to non-enterococcal streptococci (e.g. clarithromycin is two to four times more active than erythromycin). the increase in activity against staphylococci is even less striking, being restricted to a few species and limited to clarithromycin (twice as active ... | 1993 | 8478311 |
| incidence and control of campylobacter in foods. | campylobacter jejuni is known as the most common human enteropathogen in developed countries. the epidemiology of the organism has now been adequately determined and indicates that campylobacteriosis is a zoonotic disease. pets, water, and contaminated foods are the main sources of sporadic infections in humans, and no single animal food source can be excluded as a potential vehicle for infection of humans. the available information indicates that 50 to 70% of cases of enteritis in man are assoc ... | 1993 | 8484918 |
| production of hyperimmune bovine colostrum against campylobacter jejuni. | serial immunization of dairy cows with campylobacter jejuni resulted in an enhanced serum antibody response and production of hyperimmune colostrum in all vaccinated animals. an approximate 10-fold decrease in the camp. jejuni-specific antibody titres in colostrum was observed within 2 d post-partum. the lyophilized colostral concentrate fed to newborn calves resulted in a rapid increase in serum antibody response. specific camp. jejuni immunoglobulins could be detected in these animals for a fu ... | 1993 | 8486562 |
| pathological changes in the rabbit ileal loop model caused by campylobacter jejuni from human colitis. | four strains of campylobacter jejuni isolated from children with inflammatory diarrhoea were assayed in the rabbit ileal loop model of infectious diarrhoea. all caused inflammatory reactions with severe macroscopic and microscopic damage in infected rabbit ileal tissue similar to that observed in the patients by endoscopy and histological analysis of colonic biopsies. haemoglobin and other proteins were observed in loop fluids, consistent with leakage of serum from damaged mucosa. loop fluids al ... | 1993 | 8487288 |
| serologic evidence of previous campylobacter jejuni infection in patients with the guillain-barré syndrome. | to determine if patients with the guillain-barré syndrome are likely to have had campylobacter jejuni infection before onset of neurologic symptoms. | 1993 | 8489109 |
| serodiagnosis of helicobacter pylori infection in children by an indirect immunofluorescence test. | the objective of this study was to evaluate the accuracy of an indirect immunofluorescence (iif) test for serodiagnosis of helicobacter pylori infection in children and to determine how the test is affected by the presence of antibodies against campylobacter jejuni. we studied 65 consecutive children (two with endoscopically confirmed duodenal ulcer) and a series of 18 children with duodenal ulcer. thirty children were h. pylori negative, as determined by culture, by the preformed urease test, a ... | 1993 | 8492250 |
| [campylobacter jejuni sepsis in a patient with hiv infection and chronic hepatopathy]. | | 1993 | 8499522 |
| immunological activity of lipopolysaccharide of helicobacter pylori on human peripheral mononuclear blood cells in comparison to lipopolysaccharides of other intestinal bacteria. | lipopolysaccharide of helicobacter pylori was tested for its mitogenicity and for its ability to stimulate cytokine release in human peripheral blood mononuclear cells (pbmc) of healthy and h. pylori-infected blood donors. mitogenicity in pbmc induced by h. pylori lps was similar to that induced by campylobacter jejuni lipopolysaccharide, but lower than that induced by escherichia coli lipopolysaccharide in the h. pylori negative blood donor group. furthermore, h. pylori lps was able to induce t ... | 1993 | 8499895 |
| heat-labile and heat-stable haemolysins of campylobacter jejuni. | during studies on the virulence mechanisms of campylobacter jejuni clinical isolates it became apparent that some strains produced one or more haemolysins and some did not. there was no great difference between group c (cholera-like) strains and group d (dysentery-like) strains. the protein haemolysin(s) showed a spectrum of activity against erythrocytes from different animals; with maximum activity against rabbit and minimal activity against chicken erythrocytes. the results suggested a two-sta ... | 1993 | 8499897 |
| primary swine intestinal cells as a model for studying campylobacter jejuni invasiveness. | swine small-intestinal enterocytes were used to test the invasiveness of campylobacter jejuni. the cells were removed from the small intestines of 6-h-old piglets by enzymatic digestion. two clinical c. jejuni isolates invaded swine enterocytes at significantly higher frequencies than an escherichia coli control strain. the recovered colonies of c. jejuni t13192 appeared to be highly mucoid and invaded tissue culture cells (int 407) at higher frequency (0.14%) than the parental strain (0.003%). ... | 1993 | 8500914 |
| [campylobacter jejuni bacteremia and leukocytoclastic vasculitis in a cirrhotic patient]. | | 1993 | 8512981 |
| early diagnosis of typhoid fever by an enzyme immunoassay using salmonella typhi outer membrane protein preparations. | an enzyme immunoassay (eia) for detection of serum antibodies in patients with typhoid fever was developed using salmonella typhi outer membrane protein (omp) preparations as antigen. acute phase (first week) sera from adult typhoid fever patients were tested as well as sera from the following control groups: adult travellers with diarrhea caused by enterotoxigenic escherichia coli, children infected with campylobacter jejuni, healthy mexican adult blood donors, and adults with septicemia caused ... | 1993 | 8513812 |
| a campylobacter jejuni homolog of the lcrd/flbf family of proteins is necessary for flagellar biogenesis. | a campylobacter jejuni homolog of the lcrd/flbf family of genes was cloned and sequenced. the nucleotide sequence of the gene, called flba, predicted a protein of 78,864 da, with significant homology to a group of related proteins including the yersinia pestis lcrd, salmonella typhimurium inva, and caulobacter crescentus flbf proteins. the greatest homology was seen with the c. crescentus flbf protein, with an overall amino acid sequence homology of 57%. an insertion mutation in the c. jejuni 81 ... | 1993 | 8514397 |
| early colonic damage and invasion of campylobacter jejuni in experimentally challenged infant macaca mulatta. | experimental challenge studies with campylobacter jejuni were conducted in 3.5-month-old infant macaca mulatta. one infant monkey (92-1) was challenged with 2.7 x 10(10) cfu of strain 78-37. a second infant was infected intentionally by natural transmission. the infants developed diarrhea 32 h after challenge of infant 92-1. electron microscopic observations indicate that cell invasion is the primary mechanism of colon damage and diarrheal disease caused by c. jejuni. intracellular c. jejuni wer ... | 1993 | 8515112 |
| role of campylobacter jejuni flagella as colonization factors for three-day-old chicks: analysis with flagellar mutants. | campylobacter jejuni, an important cause of human gastrointestinal infection, is a major food-borne pathogen in the united states and worldwide. since poultry becomes colonized and/or contaminated during the early stages of production and is a major food-borne source for this organism, we studied the role of c. jejuni flagella on the ability of the bacterium to colonize the chicken gastrointestinal tract. three-day-old chicks were orally challenged with a motile wild-type strain of c. jejuni in9 ... | 1993 | 8517729 |
| campylobacter bacteraemia in england and wales, 1981-91. | routine surveillance of infection in england and wales detected 394 cases of campylobacter bacteraemia in 11 years. this represented an average incidence of 1.5 per 1000 intestinal campylobacter infections, with a range of 0.3/1000 in children aged 1-4 years to 5.9/1000 in patients aged 65 years or more. definitive identification of 257 isolates showed that 89% were campylobacter jejuni or c. coli; other species were c. fetus (8.6%), c. lari (0.8%), c. upsaliensis (0.8%), helicobacter (campyloba ... | 1993 | 8519321 |
| the colonization of broiler chickens with campylobacter jejuni: some epidemiological investigations. | between june 1990 and july 1991, broiler chickens from 49 flocks from 23 farms were examined for the carriage of campylobacter jejuni at slaughter. thirty-seven flocks (76%) were campylobacter-positive. prevalence of campylobacter-colonization was not associated with any of a variety of factors such as water source and broiler house floor structure. there was also no apparent seasonal variation in carriage. investigations on one farm indicated that dipping boots in disinfectant before workers en ... | 1993 | 8519325 |
| arcobacter cryaerophilus and thermophilic campylobacters in a sewage treatment plant in italy: two secondary treatments compared. | microaerophilic organisms were monitored in sewage effluent undergoing two secondary treatments: air and oxygen-activated sludge. the mean numbers of arcobacter cryaerophilus and thermophilic campylobacters detected in incoming sewage were 5639/100 ml and 1720/100 ml respectively. secondary treatment in air tanks reduced the population of a. cryaerophilus by 97.1% and of thermophilic campylobacters by 99.08%, whereas treatment in oxygen tanks reduced the bacteria 97.8% and 99.63% respectively, s ... | 1993 | 8519328 |
| [presence of virulence factors and pathovars in strains of campylobacter jejuni isolated from patients with diarrhea and healthy children]. | to study the prevalence of virulence factors (adhesion, invasion, cytotoxicity and hemolytic activity) and to establish the presence of pathovars (virulence phenotype) in c. jejuni strains isolated in pediatric patients with inflammatory and secretory diarrhea and asymptomatic carriers. | 1995 | 8519832 |
| nucleotide sequence and characterization of peb4a encoding an antigenic protein in campylobacter jejuni. | the 29-kda protein peb4, a major antigen of campylobacter jejuni, is present in all c. jejuni strains tested and elicits an antibody response in infected patients. by screening a lambda gt11 library of chromosomal dna fragments of c. jejuni strain 81-176 in escherichia coli y1090 cells with antibody raised against purified peb4, a recombinant phage with a 2-kb insert expressing an immunoreactive protein of 29 kda was isolated. dna sequence analysis revealed that the insert contains two complete ... | 1995 | 8525063 |
| efficacy of a lactic acid/sodium benzoate wash solution in reducing bacterial contamination of raw chicken. | raw chicken wings inoculated with salmonella, campylobacter jejuni, listeria monocytogenes, staphylococcus aureus, or escherichia coli o157:h7 were washed in water (control) or a solution of a 0.5% lactic acid/0.05% sodium benzoate (lb) (ph 2.64) for 30 min. viable cells of pathogenic bacteria and naturally occurring psychrotrophic bacteria on wings were enumerated after 0, 2, 4, 6, and 8 days of storage at 4 degrees c. lower populations of pathogenic and psychrotrophic bacteria were detected on ... | 1995 | 8527331 |
| emergence of multidrug resistance in campylobacter jejuni isolates from three patients infected with human immunodeficiency virus. | single-drug resistance to tetracycline, doxycycline, erythromycin, or fluoroquinolones in campylobacter isolates recovered from humans has been documented worldwide. multidrug resistance to these antibiotics is rare in campylobacter jejuni. we report the sequential development of multidrug resistance in c. jejuni isolates from three patients who were infected with human immunodeficiency virus. multiple isolates recovered from stool specimens from these patients were ribotyped, and antibiotic sus ... | 1995 | 8527556 |
| infectious gastroenteritis: are they all the same? | campylobacter jejuni and salmonella spp are the most frequently cultured micro-organisms in infectious gastroenteritis among patients hospitalized at the departments of gastroenterology and geriatrics. as a whole, the hospitalized patient population with campylobacter gastroenteritis is a younger one, compared to the salmonella-infected group. both pathogens can be associated with a biochemical pancreatitis, which is usually without clinical importance. however, serious complications can occur, ... | 1995 | 8533526 |
| quinolone resistance in clinical strains of campylobacter jejuni and campylobacter coli. | | 1995 | 8537280 |
| bactericidal properties of campylobacter jejuni-specific immunoglobulin m antibodies in commercial immunoglobulin preparations. | campylobacter jejuni is one of the most common enterocolitis-causing microorganisms worldwide. it is of particular importance in immunodeficient patients, who frequently are prone to develop extraintestinal manifestations. since these cases respond poorly to antibiotic treatment, a supportive immunomodulating therapy including the administration of c. jejuni-specific immunoglobulins would be desirable. in the present study, nine commercial immunoglobulin preparations for intravenous use were tes ... | 1995 | 8540699 |
| evidence for an efflux pump in multidrug-resistant campylobacter jejuni. | mechanisms of drug resistance in campylobacter jejuni were investigated. mutant strains 34pefr, which was resistant to pefloxacin (128-fold increase in the mic), and 34ctxr, which was resistant to cefotaxime (32-fold increase in the mic) and which was derived from the susceptible parent 34s, were obtained by serial passages on pefloxacin and cefotaxime gradient plates, respectively. both mutants showed cross-resistance to erythromycin, chloramphenicol, tetracycline, beta-lactams, and quinolones. ... | 1995 | 8540709 |
| secretory monoclonal iga class-switch variants against bacterial enteric pathogens in bile and intestinal secretions. | in a previous study we analyzed the molecular forms of monoclonal iga class-switch variants (moiga variants) and their transport into murine respiratory secretions. the aim of the present study is to characterize the transport of moiga variants into bile and intestinal secretions so that their applicability in a passive immunization model of the gut can be evaluated. different moiga variants were directly isolated from igg1 and igg2a producing hybridoma clones specific for the same surface deter ... | 1995 | 8541811 |
| bilateral ptosis, tonic pupils and abducens palsies following campylobacter jejuni enteritis. | | 1995 | 8543096 |
| [septic abortion in campylobacter jejuni infection]. | case report of a 24 year old woman ii g/i p with enterocolitis and septicaemia caused by campylobacter jejuni (c.) in the following time abortion at 16 weeks of gestation. diagnostics, therapy, review of literature. | 1995 | 8543136 |
| [campylobacter jejuni and autoimmune-mediated neurologic diseases: pathogenesis of guillain-barré syndrome and fisher's syndrome]. | | 1995 | 8544349 |
| mechanisms of resistance to fluoroquinolones: state-of-the-art 1992-1994. | this paper gives an update on the mechanisms of bacterial resistance to fluoroquinolones. the laboratory techniques currently used to determine the mechanism(s) of resistance are outlined, including the use of restriction fragment length polymorphism and single-stranded conformational polymorphism analysis of mutations in gyra. alterations in gyra have continued to be the most reported cause of resistance, with high level resistance due to 2 or more mutations in this gene. recently, mutations in ... | 1995 | 8549336 |
| cloning and membrane topology of a p type atpase from helicobacter pylori. | southern blot screening of a genomic helicobacter pylori library was employed to find a p type atpase using a mixture of 16 dna oligonucleotides coding for the dktgt(i/l)t consensus sequence specific for the phosphorylation site of this family of atpases. a positive clone, prh439, was isolated and sequenced. the inserted 3.4-kb h. pylori dna contained an intact open reading frame encoding a protein of 686 amino acids carrying the consensus sites for phosphorylation and atp binding. the amino aci ... | 1996 | 8550601 |
| evaluation of oxyrase enrichment method for isolation of campylobacter jejuni from inoculated foods. | recovery limits were evaluated for campylobacter jejuni in an existing food and drug administration (fda) enrichment broth (eb) formula supplemented with oxyrase enzyme. cultures of camp. jejuni were inoculated into eb or eb containing 10% raw milk, raw oysters, crabmeat or mushrooms. after 24 and 48 h of enrichment, camp. jejuni was isolated on four selective agars. no significant differences in recovery rates for camp. jejuni were observed in the oxyrase enrichment under normal atmosphere or i ... | 1995 | 8554759 |
| the speciation and subtyping of campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular techniques. | in this study the distribution of phenotypes of campylobacter strains in sewage and surface waters was investigated by subtyping and by speciation of isolates from various aquatic environments. these environments included two municipal sewage plants (spa and spb) and waste water from a poultry abattoir (wwa). both the sewage plants spa and spb collected domestic and industrial waste, and spa received drain water from wwa. spb received no waste water from any meat-processing plant. the isolates w ... | 1995 | 8557080 |
| helicobacter pylori increases proliferation of gastric epithelial cells. | the direct and indirect effects of helicobacter pylori on cell kinetics of gastric epithelial cell line ags were investigated by flow cytometric analysis of ki-67 positive cells and by mtt assay. flow cytometric analysis of ki-67 positivity permits detection of cells that are in s-phase, whereas the mtt assay is a colometric measure of the number of viable cells. in the absence of added stimulants, 23.06 (4.88)% mean (sd) of ags cells were ki-67 positive. when cells were preincubated in the pres ... | 1996 | 8566853 |
| evaluation of a pcr based assay for specific detection of campylobacter jejuni in chicken washes. | an assay for campylobacter jejuni based on the polymerase chain reaction was developed in our laboratory and shown to be a sensitive and specific method to identify this bacterium in pure culture. this assay was evaluated as a method to rapidly detect c. jejuni attached to chicken carcasses. chicken carcasses were sampled for pcr using three methods including pre-enrichment of the washes, direct plating of the washes and differential centrifugation of the washes prior to testing. it was found th ... | 1995 | 8569769 |
| culture and characteristics of helicobacter bizzozeronii, a new canine gastric helicobacter sp. | organisms whose cells were large, tight spirals were isolated from gastric biopsies of dogs. touch cytology samples from all of the dogs contained large spiral organisms. characteristics of 10 strains are described. these organisms were 5 to 10 microns long by 0.3 microns wide, and each cell had 10 to 20 sheathed flagella at both ends of the cell. the cells did not have periplasmic fibrils. these organisms were microaerophilic and grew at 37 and 42 degrees c but not at 25 degrees c on brain hear ... | 1996 | 8573490 |
| identification and characterization of an immunogenic outer membrane protein of campylobacter jejuni. | we cloned and expressed in escherichia coli a gene encoding an 18-kda outer membrane protein (omp18) from campylobacter jejuni atcc 29428. the nucleotide sequence of the gene encoding omp18 was determined, and an open reading frame of 165 amino acids was revealed. the amino acid sequence had the typical features of a leader sequence and a signal peptidase ii cleavage site at the n-terminal part of omp18. moreover, the sequence had a high degree of similarity to the peptidoglycan-associated outer ... | 1995 | 8576327 |
| the purification of a groel-like stress protein from aerobically adapted campylobacter jejuni. | from plate cultures of campylobacter jejuni grown in room air a particulate protein of 62 kda was isolated by ion-exchange chromatography. the protein had a square shape from the side view but when viewed from the top it had a star-shaped structure. the molecular size of the whole particle determined by gel filtration was 850 kda which suggested the presence of 14 subunits of 62 kda in each particle. the n-terminal 37 amino residues showed more than 80% homology with the sequence of these heat s ... | 1995 | 8577276 |
| binding of outer membrane preparations of campylobacter jejuni to int 457 cell membranes and extracellular matrix proteins. | binding of outer membrane (om) preparations of the thermophilic campylobacter species c. jejuni to epithelial cell membranes and extracellular matrix proteins were studied in an in vitro model system using enzyme-linked immunosorbent assay. the om preparations exhibited significant binding to int 407 intestinal cell membranes. the process of adhesion was modulated by enzymatic, chemical or immunological pretreatment of the bacteria. following oxidation of the lipopolysaccharide (lps) with sodium ... | 1995 | 8577316 |
| random amplified polymorphic dna fingerprinting of campylobacter jejuni and c. coli isolated from human faeces, seawater and poultry products. | the polymerase chain reaction (pcr) technique was used to obtain randomly amplified polymorphic dna (rapd) profiles from 64 type and serotype reference strains and 114 isolates of campylobacter jejuni and c. coli from food, seawater and human faeces. genetic diversity was detected among the strains as a total of 118 different rapd profiles were obtained, each one containing from 4 to 11 bands between 0.30 and 1.50 kb. the discriminatory power of a random 10-mer primer (sequence 5'-caatcgccgt-3') ... | 1995 | 8584791 |
| antibodies to campylobacter jejuni react with myelin proteins of the central nervous system. | | 1995 | 8586851 |
| the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species. | the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ... | 1995 | 8587109 |
| in ovo oral vaccination with campylobacter jejuni establishes early development of intestinal immunity in chickens. | 1. chick embryos were orally immunised at day 16 of incubation by injection of heat-killed campylobacter jejuni organisms into the amniotic fluid. the response to vaccination was observed at 5 d after hatching or, in some birds which received a postnatal oral booster vaccination, at 7 d after hatching, and the response was observed at 14 d of age. 2. the titres of antibody in serum, bile and intestinal scrapings, the distribution of immunoglobulin-containing cells in the spleen, duodenum and ile ... | 1995 | 8590089 |
| campylobacter jejuni infection and guillain-barre syndrome. | | 1996 | 8592566 |
| surveillance for waterborne-disease outbreaks--united states, 1993-1994. | since 1971, cdc and the u.s. environmental protection agency have maintained a collaborative surveillance system for collecting and periodically reporting data that relate to occurrences and causes of waterborne-disease outbreaks (wbdos). | 1996 | 8600346 |
| traveler's diarrhea, campylobacter jejuni, and acute motor axonal neuropathy. | | 1996 | 8602770 |
| cloning, sequencing, and expression of a gene from campylobacter jejuni encoding a protein (omp18) with similarity to peptidoglycan-associated lipoproteins. | a campylobacter jejuni genomic plasmid library was screened with antiserum generated against whole c. jejuni, revealing two immunoreactive clones. sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 da. the recombinant product partitioned to the outer membrane fractions of escherichia coli transformants and has been designated omp18. the deduced amino acid sequence o ... | 1996 | 8613402 |
| multifocal motor neuropathy with conduction block and campylobacter jejuni. | we describe a patient with acute multifocal motor neuropathy with conduction block (mmncb) and high titers of immunoglobulin g anti-gm1 antibodies after campylobacter jejuni enteritis. treatment with intravenous immune globulin led to rapid improvement with return of normal function by 6 weeks. this is the first report of c. jejuni enteritis preceding mmncb. | 1996 | 8614535 |
| [the comparative identification of campylobacter strains by traditional enzymatic tests and the gene amplification reaction]. | 39 strains of campylobacter isolated from 153 diarrhoeal children (0-3 years) were comparatively identified by the traditional enzymatic tests and by the polymerase chain reaction (pcr). the hippurate hydrolysis test appreciated 27 strains as campylobacter jejuni (69.2%) and 12 strains as campylobacter coli (30.8%). the p.c.r. realised in france has appreciated 29 strains as campylobacter jejuni (74.4%) and 10 strains as campylobacter coli (25.6%). the analysis of the results discrepancy permitt ... | 1995 | 8640005 |
| molecular cloning and site-specific mutagenesis of a gene involved in arylsulfatase production in campylobacter jejuni. | the arylsulfatase gene from campylobacter jejuni 81-176 encodes a predicted protein of 69,293 da which shows no sequence similarity with other known arylsulfatases. the gene hybridizes to other ast+ strains of c. jejuni and campylobacter sputorum subsp. bubulus, as well as to many ast- strains of c. jejuni. | 1996 | 8655516 |
| [axonal guillain-barré syndrome associated with anti-galnac-gd1a antibody subsequent to campylobacter jejuni (pen 43) enteritis]. | we reported a 16-year-old boy who had guillain-barré syndrome (gbs) after suffering diarrhea. campylobacter jejuni was isolated from his stool, and the serotype belonged to pen 43. neurologic examination revealed distal-dominant muscle weakness atrophy, and mild sensory disturbance. motor and sensory nerve conduction velocities were normal, but compound muscle action potentials were markedly reduced. serum from the patient had high titers of anti-falnac-gd1a antibodies. he had hla-a24, b51, drb1 ... | 1995 | 8665735 |
| an outbreak of campylobacter jejuni enteritis associated with failed milk pasteurisation. | this paper describes an outbreak of gastrointestinal illness affecting at least 110 people, of whom 41 had microbiological confirmation of campylobacter jejuni infection. the outbreak of infection was found to have been associated with consumption of inadequately pasteurised milk from a local dairy. the problem of enforcement of food and safety regulations when milk from dairies fails the phosphatase test is discussed. the prevalence of seroconversion to campylobacter in the community is estimat ... | 1995 | 8666844 |
| polymerase chain reaction assay for detection of campylobacter coli and campylobacter jejuni in poultry meat. | primers of 16s rrna and flaa genes were used to optimise a pcr technique for detecting thermotolerant campylobacters in poultry meat. different methods for crude dna extraction were also evaluated. the use of flaa primers and extraction of nucleic acid by boiling and proteinase k gave good results in the detection of campylobacter either in artificially or naturally contaminated foodstuffs. the lowest sensitivity limit for the pcr reaction was 10(1)-10(2) thermophilic campylobacter cells either ... | 1995 | 8672221 |
| detection of serum-dependent cytotoxic activity of campylobacter jejuni and its characteristics. | we previously set forth appropriate three assay systems using chinese hamster ovary cells to detect the campylobacter jejuni cytotoxin. although we could not reach a conclusion because the cytotoxins shown in this study were not purified, at least three different cytotoxins were detected in these assay systems. the first cytotoxin in the presence of fetal calf serum (fcs) was heat-labile and the molecular weight (mw) was estimated at 50-100 k by ultrafiltration. the second cytotoxin detected in ... | 1996 | 8672594 |
| prevalence of cytolethal distending toxin production in campylobacter jejuni and relatedness of campylobacter sp. cdtb gene. | campylobacter jejuni produces a toxin called cytolethal distending toxin (cdt). the genes encoding this toxin in c. jejuni 81-176 were cloned and sequenced. the nucleotide sequence of the genes revealed that there are three genes, cdta, cdtb, and cdtc, encoding proteins with predicted sizes of 30,11-6, 28,989, and 21,157 da, respectively. all three proteins were found to be related to the escherichia coli cdt proteins, yet the amino acid sequences have diverged significantly. all three genes wer ... | 1996 | 8675309 |
| restriction fragment length polymorphism of polymerase chain reaction products applied to the differentiation of poultry campylobacters for epidemiological investigations. | a technique for subtyping campylobacter jejuni isolates has been developed by using the restriction fragment length polymorphism (rflp) of polymerase chain reaction (pcr) products of the flaa and flab genes. the technique was validated by using strains representing 28 serotypes of c jejuni and it may also be applied to c coli. from these strains 12 distinct rflp profiles were observed but there was no direct relationship between the rflp profile and the serotype. one hundred and thirty-five camp ... | 1996 | 8685540 |
| campylobacter jejuni/coli and enterotoxigenic escherichia coli (etec) in faeces from children and adults in tanzania. | the occurrence of campylobacter and enterotoxigenic e. coli (etec) was studied in faecal samples from tanzanian children (< 5 years of age), adolescents and adults (only campylobacter) with and without diarrhoea. the campylobacter strains isolated were tested for subspecies, enterotoxigenicity and serotype. out of 394 children with diarrhoea 18% were infected with campylobacter and 20% with etec. in 278 samples tested for campylobacter and 136 tested for etec from asymptomatic children the corre ... | 1995 | 8685639 |
| identification and characterization of genus-specific epitopes of serpulina species using monoclonal antibodies. | four murine monoclonal antibodies (mabs) designated as c9e8, a10, g12, and g8 which recognized both serpulina hyodysenteriae and s. innocens were produced and characterized. the mabs reacted with whole cell antigens in elisa, indirect immunofluorescence and immunoblot assays. the mabs did not show any cross reactivity in rapid dot elisa or immunoblot assay with leptospira icterohemorrhagiae, campylobacter jejuni and escherichia coli. treatment of whole cell suspension with proteinase k and sodiu ... | 1996 | 8701579 |
| [isolation of a strain of campylobacter jejuni resistant to fluoroquinolones and sensitive to nalidixic acid]. | | 1996 | 8714164 |
| [bacteremia caused by campylobacter jejuni associated with spontaneous abortion]. | | 1996 | 8714192 |
| in vitro studies of campylobacter jejuni/coli strains from hens and humans regarding adherence, invasiveness, and toxigenicity. | campylobacter jejuni/coli strains from hens and humans were compared for their ability to adhere to and invade hep-2-cells and for toxigenicity to cho-cells. in both hen and human strains, invasiveness was higher among non-toxigenic strains than among toxigenic ones. the frequency of adherence, invasiveness, and toxigenicity was the same in hen and human strains. | 1995 | 8719204 |
| a genetic hybrid of the campylobacter jejuni flaa gene with lt-b of escherichia coli and assessment of the efficacy of the hybrid protein as an oral chicken vaccine. | the objectives of this study were to produce campylobacter jejuni flagellin fused to the b-subunit of the labile toxin (lt-b) of escherichia coli and to assess the efficacy of the hybrid protein as a chicken vaccine. part of the flaa gene (780 base pairs) was cloned in plasmid pbeb downstream and in frame with the lt-b to allow expression of a hybrid protein. transformed e. coli chi 6097 expressed the hybrid protein (43 kdaltons) in inclusion bodies at mid log phase. the inclusion bodies were is ... | 1995 | 8719215 |
| ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study. | from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ... | 1996 | 8722540 |
| restriction fragment length polymorphism of flagellin genes of campylobacter jejuni and/or c. coli isolates from egypt. | the conservation of flagellin genes from thermophilic campylobacter spp. strains isolated in egypt was evaluated by a restriction fragment length polymorphism (rflp) assay. the flaa and flab genes were amplified from 59 independent clinical isolates and digested with ecori and psti, and the resulting patterns were compared with each other and with previously described rflp groups. the results indicate that the isolates fell into 14 groups for flaa and 11 groups for flab, 9 of which have been des ... | 1996 | 8727905 |
| detection of ciprofloxacin resistance mutations in campylobacter jejuni gyra by nonradioisotopic single-strand conformation polymorphism and direct dna sequencing. | a total of 27 strains of campylobacter jejuni (24 clinical strains and three laboratory strains) were examined for the presence of point mutations in the quinolone resistance determining region (qrdr) of gyra gene by nonradioisotopic single-strand conformation polymorphism (non-ri sscp) analysis with silver stain. direct dna sequencing of the polymerase chain reaction (pcr)-amplified dna fragments confirmed the results obtained by non-ri sscp analysis and revealed that in clinical strains high-l ... | 1996 | 8731499 |
| antimicrobial activity of chicken and turkey heterophil peptides chp1, chp2, thp1, and thp3. | four avian heterophil antimicrobial cationic peptides (chicken heterophil peptides 1 and 2, and turkey heterophil peptides 1 and 3) were evaluated for in vitro microbicidal activity against selected avian pathogens and human pathogens which are harbored by birds. at concentrations of 16-2 micrograms/ml, all four avian peptides effected a greater than 90% reduction in the survival of candida albicans, salmonella enteriditis, and campylobacter jejuni. none of the peptides, including the known anti ... | 1995 | 8748545 |
| interactions between campylobacter jejuni and lipids. | we previously showed that motility plays several key roles in campylobacter jejuni pathogenesis, including increasing the efficiency of c. jejuni attachment to host epithelial cells. to further characterize c. jejuni attachment, we first examined the role of carbohydrates. experiments with chinese hamster ovary (cho) cell mutants with defined defects in complex carbohydrate biosynthesis revealed that oligosaccharide sequences probably play a subordinate role in c. jejuni attachment to eukaryotic ... | 1996 | 8751886 |
| molecular characterization of a campylobacter jejuni 29-kilodalton periplasmic binding protein. | campylobacter jejuni, a gram-negative, microaerophilic, spiral bacterium, is a common cause of human gastrointestinal disease. although investigators commonly use c. jejuni glycine-hydrochloride extracts in assays to determine the products that promote the binding of the organism to eukaryotic cells, the proteins contained within these extracts remain ill defined. characterization of these proteins will provide a better understanding of c. jejuni gene regulation and organization. an antiserum wa ... | 1996 | 8751896 |
| [pathogenesis of guillain-barré syndrome and fisher's syndrome: molecular mimicry between antecedent infectious agents and components of nerve tissues]. | sera from patients with guillain-barré syndrome (gbs) following campylobacter jejuni infection have autoantibody to gm1 ganglioside. we have investigated whether gm1-oligosaccharide structure is present in the lipopolysaccharide (lps) of c. jejuni (pen 19) that was isolated from a gbs patient. the lps showing the binding activity of cholera toxin, which specifically recognizes the gm1-oligosaccharide, was purified by a column chromatography. proton nmr methods have revealed that the lps has the ... | 1995 | 8752400 |
| miller-fisher syndrome associated with campylobacter jejuni bearing lipopolysaccharide molecules that mimic human ganglioside gd3. | a campylobacter jejuni strain of serotype o:10 was isolated from a patient who had miller-fisher syndrome. in its biochemical reactions and cellular morphology, the isolate was characteristic of typical c. jejuni. antibodies against extracted lipopolysaccharide (lps) were detected by passive hemagglutination in the acute- and convalescent-phase patient sera. by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with the o:10 antiserum, it was demonstrated that the strai ... | 1996 | 8757818 |
| increased colonization potential of campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks. | the mechanisms by which campylobacter jejuni rapidly spreads through large broiler flocks are unknown. recent evidence suggests that the minimum infective oral dose for 100% caecal colonization is about 10(4) cfu, which, with such a 'fragile' organism, may limit transmissibility. however, the colonization potential of laboratory-adapted strains may be anomalous compared with fresh isolates or those passaged in vivo. the colonization potential of two chicken and one human c. jejuni isolates, whic ... | 1996 | 8760971 |
| campylobacter jejuni infections and anti-gm1 antibodies in guillain-barré syndrome. | the group of patients with guillain-barr'e syndrome (gbs) is very heterogenous with regard to antecedent infections, immunological parameters, clinical manifestations, and response to treatment. in this study, the presumed pathogenic factors anti-gm1 antibodies and campylobacter jejuni infections were related to the clinical characteristics. serum from 154 patients with gbs, 63 patients with other neurological diseases (ond), and 50 normal controls (nc) were tested for the presence of antibodies ... | 1996 | 8773599 |
| experimental hepatitis induced by campylobacter jejuni infection in japanese quail (coturnix coturnix japonica). | to establish an experimental model for vibrionic hepatitis caused by campylobacter jejuni, japanese quails (coturnix coturnix japonica) were inoculated with c. jejuni strains isolated from chicken hepatitis (bl107) and human diarrhea (hp5113). necrotic liver lesions were formed by intra-pancreaticoduodenal vein injection by which the bacteria reached the liver directly via the portal vein, but not by intra-gastric infection. these liver lesions were observed from day 1 to 7 after the infection. ... | 1996 | 8777226 |
| infection with ciprofloxacin-resistant campylobacter jejuni in travellers returning from asia. | | 1996 | 8781891 |
| antimicrobial activity of rifabutin. | rifabutin is a spiro-piperidyl-rifamycin derived from rifamycin-s. it is structurally related to rifampin and shares many of its properties. rifabutin has a broad spectrum of antimicrobial activity. it is considerably more active than rifampin in vitro against the mycobacterium avium complex (mac), mycobacterium tuberculosis, and mycobacterium leprae. it also is active against most atypical mycobacteria, including mycobacterium kansasii, but mycobacterium chelonae is relatively resistant. rifabu ... | 1996 | 8785253 |
| comprehensive ribotyping scheme for heat-stable serotypes of campylobacter jejuni. | strains from diverse sources belonging to all 47 heat-stable penner serotypes of campylobacter jejuni were examined for polymorphism around the 16s rrna genes. penner serotype reference strains and a group of nonserotypeable isolates were included in the study. complete typeability was obtained; 30 distinct psti and 42 haeiii polymorphisms were found. three bands were detected in almost all strains with these enzymes, confirming that three copies of the 16s rrna gene are typical for c.jejuni. by ... | 1996 | 8788998 |
| analysis of hl and o serotypes of campylobacter strains by the flagellin gene typing system. | we recently developed a molecular typing system for campylobacter jejuni and campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaa (i.nachamkin, k. bohachick, and c.m. patton, j. clin. microbiol. 31:1531-1536, 1993). we extended the typing system to 83 flagellin types (designated flaa-1,flaa-2, etc.) on the basis of analysis of 404 isolates of c. jejuni and c. coli including common serotypes isolated in the united states, a selection of less com ... | 1996 | 8789000 |
| an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis. | examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ... | 1996 | 8793884 |
| experimental campylobacter jejuni infection in the chicken: an animal model of axonal guillain-barré syndrome. | to develop and characterise an animal model of paralytic neuropathy after campylobacter jejuni infection. campylobacter infection precedes development of many cases of guillain-barré syndrome and is particularly associated with cases having prominent axonal degeneration. understanding the pathogenesis of guillain-barré syndrome after c jejuni infection has been slowed by the lack of animal models. | 1996 | 8795599 |
| cytomegalovirus infection and guillain-barré syndrome: the clinical, electrophysiologic, and prognostic features. dutch guillain-barré study group. | guillain-barré syndrome (gbs) is usually preceded by infections, in particular cytomegalovirus (cmv) and campylobacter jejuni infection. we studied the clinical and electrophysiologic features of 20 cmv-associated gbs patients and compared the findings with earlier established data of c. jejuni-related gbs patients (n = 43) and of gbs patients without these infections (n = 71). the patients all participated in the dutch gbs trial in which we compared the effect of intravenous immune globulins an ... | 1996 | 8797462 |
| transcription of the campylobacter jejuni cell division gene ftsa. | as part of a study of genes whose transcription is maintained in stationary phase, cloned segments of dna were selected from a lambda zap ii library of campylobacter jejuni nctc 11,168. one such clone was found to encode a homologue of the escherichia coli cell division gene ftsa. examination of mrna by transcription mapping revealed that the campylobacter gene has one major and three minor transcription start sites. there were several significant differences in the structure and organisation of ... | 1996 | 8807806 |
| [the e test and quinolone-resistant campylobacter jejuni]. | the e-test is a quantitative technique for the determination of antimicrobial sensitivity. the system is based on the diffusion of a predefined exponential gradient in a determined antibiotic, using an inert plastic support which is applied to a solid culture medium and it is an alternative method for antibiotic sensitivity testing. of the series published, some specifically include microorganisms considered fastidious or difficult to grow, such as campylobacter jejuni. in recent years a progres ... | 1995 | 8808474 |
| construction of a ferritin-deficient mutant of campylobacter jejuni: contribution of ferritin to iron storage and protection against oxidative stress. | the ferritin-encoding gene (cft) of campylobacter jejuni was cloned and sequenced. the nucleotide sequence of cft had a 501 bp open reading frame for a protein with 167 amino acids and a predicted molecular mass of 19 180 da, and showed a high similarity to that of helicobacter pylori and escherichia coli ferritin genes. to determine the biological function of ferritin in c. jejuni, a ferritin-deficient mutant was constructed. the growth of ferritin-deficient strain sna 1 was clearly inhibited u ... | 1996 | 8809765 |
| expanded genomic map of campylobacter jejuni ua580 and localization of 23s ribosomal rrna genes by i-ceui restriction endonuclease digestion. | the genomic map of campylobacter jejuni ua580 was expanded and more precisely constructed using i-ceui, sal/i and smai restriction endonucleases in conjunction with pulsed-field gel electrophoresis (pfge). the presence of three fragments after digestion with i-ceui confirmed the presence of three copies of the 23s ribosomal rrna (rrl) gene. the genome size of campylobacter jejuni ua580 was determined to be 1725 +/- 5.9 kbp by i-ceui with fragment sizes of 1053 +/- 4.4, 361 +/- 2.7 and 311 +/- 3. ... | 1996 | 8810506 |
| dna fingerprinting and serotyping of campylobacter jejuni isolates from epidemic outbreaks. | the aim of the present investigation was to compare dna fingerprinting and serotyping (heat-stabile and heat-labile antigens) of isolates from epidemic outbreaks as well as of solitary isolates. campylobacter jejuni isolates from two epidemic outbreaks in sweden, one milkborne (35 isolates) and one waterborne (17 isolates), and one waterborne outbreak in norway (11 isolates), as well as 30 solitary isolates from swedish patients with gastroenteritis, were analyzed. a total of 93 isolates were an ... | 1996 | 8815103 |
| a rapid, colourimetric assay for cytotoxin activity in campylobacter jejuni. | cell extracts and culture supernates of campylobacter jejuni nctc 11168 and three isolates from faecal samples from patients with enteritis were tested for cytotoxic activity on hela and vero cells using a sensitive and rapid dye reduction assay which represents a simple assay for cytotoxin activity that can be assessed visually or spectrophotometrically in the wells of microplates. the assay was as sensitive as trypan blue exclusion and did not require the use of radioisotopes. a low level of c ... | 1996 | 8821400 |
| fatal septic shock with multiple organ failure due to campylobacter jejuni. | | 1996 | 8824999 |
| antineutrophil cytoplasmic antibodies in reactive arthritis. | to study the occurrence of antineutrophil cytoplasmic antibodies (anca) in reactive arthritis (rea). | 1995 | 8835566 |
| a study of nosocomial diarrhea in calcutta. | nosocomial infection is a major problem in hospitalized patients, particularly those who are debilitated. these infections may manifest as diarrhea. the spectrum of infections agents causing nosocomial diarrhea in our country is not known. | 1996 | 8840619 |
| detection of campylobacter jejuni/c.coli infection in patients with guillain-barré syndrome by serology and culture. | serum samples from 43 cases of guillain-barré syndrome (gbs), 32 non-gbs neurology patients, and 35 healthy persons from a medical institute in kerala, india, were tested for antibody levels against a campylobacter jejuni strain penner serotype 0:19 by agglutination and elisa. twenty-six percent of samples from gbs cases showed high antibody levels in all assays. of 8 stool samples of new gbs cases examined by culture, 38% were positive for c. jejuni/c. col1. the results suggest that at least a ... | 1996 | 8841044 |
| molecular typing of thermotolerant species of campylobacter with ribosomal rna gene patterns. | ribosomal rna gene restriction patterns (ribopatterns) of 72 strains representing campylobacter jejuni (subspecies jejuni and doylei), c. coli, c. upsaliensis and c. lari including the urealytic (uptc) biovar were determined using four common restriction endonucleases (haeiii, hindiii, psti and pvuii). the relative effectiveness of these enzymes for general molecular typing of the thermotolerant campylobacters was assessed. ribotypes (haeiii) were defined on the basis of computer-assisted numeri ... | 1993 | 8190997 |
| campylobacter jejuni bacteremia and guillain-barré syndrome in a patient with gvhd after allogeneic bmt. | guillain-barré syndrome is a rare neurologic complication after allogeneic bmt. in the non-transplant setting, guillain-barré syndrome has typically been associated with antecedent acute infections and numerous reports have suggested an association between campylobacter jejuni infection and the subsequent development of guillain-barré syndrome. thus far, however, reports of c. jejuni-associated guillain-barré syndrome have been limited to gastrointestinal c. jejuni infections and none has been r ... | 1994 | 8199580 |