| evaluation of anthelmintic resistance and exhaust air dust pcr as a diagnostic tool in mice enzootically infected with aspiculuris tetraptera. | the entry of infectious agents in rodent colonies occurs despite robust sentinel monitoring programs, strict quarantine measures, and stringent biosecurity practices. in light of several outbreaks with aspiculuris tetraptera in our facilities, we investigated the presence of anthelmintic resistance and the use of exhaust air dust (ead) pcr for early detection of a. tetraptera infection. to determine anthelmintic resistance, c57bl/6, dba/2, and ncr nude mice were experimentally inoculated with em ... | 2017 | 28535863 |
| cholinergic signaling plasticity maintains viscerosensory responses during aspiculuris tetraptera infection in mice small intestine. | intestinal parasites alter gastrointestinal (gi) functions like the cholinergic function. aspiculuris tetraptera is a pinworm frequently observed in laboratory facilities, which infests the mice cecum and proximal colon. however, little is known about the impact of this infection on the gi sensitivity. here, we investigated possible changes in spontaneous mesenteric nerve activity and on the mechanosensitivity function of worm-free regions of naturally infected mice with a. tetraptera. infection ... | 2017 | 28641950 |
| intestinal helminths in different species of rodents in north khorasan province, northeast of iran. | rodents are an important source of zoonotic diseases for human. the aim of this study was to determine the infectivity of rodents with intestinal helminths in north khorasan province, iran. | 2017 | 28761488 |
| comparative analyses of the complete mitochondrial genomes of the two murine pinworms aspiculuris tetraptera and syphacia obvelata. | pinworms aspiculuris tetraptera and syphacia obvelata are important parasitic nematodes of laboratory mice, rat and other rodents. however, the mitochondrial (mt) genome of these parasites have not been known yet. in the present study, the complete mt genomes of a. tetraptera and s. obvelata were sequenced, which were 13,669 bp and 14,235 bp in size, respectively. both genomes included 12 protein-coding genes, two rrna genes, 22 trna genes and one non-coding region. the mt genomes of a. tetrapte ... | 2016 | 27016298 |
| guidance regarding sample collection and refinement of fecal flotation exam for the isolation of aspiculuris tetraptera. | aspiculuris tetraptera continues to be a problem in rodent vivaria, in part due to difficulties in parasite detection. although pcr testing is highly sensitive, it is expensive and does not always provide immediate results. consequently, many institutions rely on passive fecal flotation as a quick inhouse exam for diagnosing a. tetraptera infections. to increase the sensitivity of this test, we examined multiple parameters to determine the optimal test protocol. a 30-min soaking period prior to ... | 2016 | 27657708 |
| parasites and invasions: changes in gastrointestinal helminth assemblages in invasive and native rodents in senegal. | understanding why some exotic species become widespread and abundant in their colonised range is a fundamental issue that still needs to be addressed. among many hypotheses, newly established host populations may benefit from a parasite loss ("enemy release" hypothesis) through impoverishment of their original parasite communities or reduced infection levels. moreover, the fitness of competing native hosts may be negatively affected by the acquisition of exotic taxa from invaders ("parasite spil ... | 2016 | 27670366 |
| comparison of traditional and pcr methods during screening for and confirmation of aspiculuris tetraptera in a mouse facility. | pinworm detection in laboratory rodents typically is accomplished by using the tape test or various modifications of fecal flotation test to detect eggs. direct examination of intestinal contents remains the 'gold standard' for pinworm detection, with the limitation of euthanasia of animals. here, we compare traditional and real-time pcr methodologies during screening for and confirming the presence of aspiculuris tetraptera. two sets of pooled fecal samples collected from each of 521 microisola ... | 2011 | 22330785 |