| human papillomavirus 18 as a poor prognostic factor in stage i-iia cervical cancer following primary surgical treatment. | this study evaluates the effect of the specific human papillomavirus (hpv) genotype as a prognostic factor in stage i-iia cervical cancer patients following primary surgical treatment. | 2014 | 25469338 |
| human papillomavirus genotype distribution in cervical intraepithelial neoplasia grade 2/3 and invasive cervical cancer in japanese women. | human papillomavirus vaccines are being introduced worldwide and are expected to reduce the incidence of cervical cancer. here we report a cross-sectional study using a validated human papillomavirus genotyping method to reveal the human papillomavirus prevalence and genotype distribution in japanese women with cervical intraepithelial neoplasia grade 2/3 and invasive cervical cancer. | 2014 | 25156680 |
| comprehensive high-throughput rna sequencing analysis reveals contamination of multiple nasopharyngeal carcinoma cell lines with hela cell genomes. | in an attempt to explore infectious agents associated with nasopharyngeal carcinomas (npcs), we employed our high-throughput rna sequencing (rna-seq) analysis pipeline, rna compass, to investigate the presence of ectopic organisms within a number of npc cell lines commonly used by npc and epstein-barr virus (ebv) researchers. sequencing data sets from both cne1 and hone1 were found to contain reads for human papillomavirus 18 (hpv-18). subsequent real-time reverse transcription-pcr (rt-pcr) anal ... | 2014 | 24991015 |
| a dna methylation classifier of cervical precancer based on human papillomavirus and human genes. | testing for high-risk (hr) types of human papillomavirus (hpv) is highly sensitive as a screening test of high-grade cervical intraepithelial neoplastic (cin2/3) disease, the precursor of cervical cancer. however, it has a relatively low specificity. our objective was to develop a prediction rule with a higher specificity, using combinations of human and hpv dna methylation. exfoliated cervical specimens from colposcopy-referral cohorts in london were analyzed for dna methylation levels by pyros ... | 2014 | 24535756 |
| papillary squamous cell carcinoma of the trachea associated with human papillomavirus-18 infection. | a 75-year-old man presented with the chief complaints of coughing and dyspnea. chest computed tomography revealed narrowing of the trachea. a tumor measuring 40×33×15 mm in size that caused -90% reduction in the cross-sectional area of the trachea 4 cm above the carina was cauterized with high-frequency electrocautery via a rigid bronchoscope. a pathological examination revealed papillary squamous cell carcinoma with immunohistochemical p16 overexpression. multiplex polymerase chain reaction con ... | 2013 | 24334586 |
| optimization of virus detection in cells using massively parallel sequencing. | massively parallel sequencing (mps)-based virus detection has potential regulatory applications. we studied the ability of one of these approaches, based on degenerate oligonucleotide primer (dop)-polymerase chain reaction (pcr), to detect viral sequences in cell lines known to express viral genes or particles. dop-pcr was highly sensitive for the detection of small quantities of isolated viral sequences. detected viral sequences included nodavirus, bracovirus, and endogenous retroviruses in hig ... | 2014 | 24309095 |
| a low density microarray method for the identification of human papillomavirus type 18 variants. | we describe a novel microarray based-method for the screening of oncogenic human papillomavirus 18 (hpv-18) molecular variants. due to the fact that sequencing methodology may underestimate samples containing more than one variant we designed a specific and sensitive stacking dna hybridization assay. this technology can be used to discriminate between three possible phylogenetic branches of hpv-18. probes were attached covalently on glass slides and hybridized with single-stranded dna targets. p ... | 2013 | 24077317 |
| evolution and taxonomic classification of alphapapillomavirus 7 complete genomes: hpv18, hpv39, hpv45, hpv59, hpv68 and hpv70. | the species alphapapillomavirus 7 (alpha-7) contains human papillomavirus genotypes that account for 15% of invasive cervical cancers and are disproportionately associated with adenocarcinoma of the cervix. complete genome analyses enable identification and nomenclature of variant lineages and sublineages. | 2013 | 23977318 |
| prognostic value of human papillomavirus types 16 and 18 dna physical status in cervical intraepithelial neoplasia. | the aim of this work was to assess the value of the physical status of human papillomavirus (hpv) dna as a disease marker for cervical cancer development in a set of 248 dna samples previously genotyped as hpv 16 or 18, by calculating the e2/e6 ratio through real-time pcr. there was a significant difference in integration status according to disease grade for both genotypes (p <0.001). furthermore, especially for hpv 18, determining the dna physical status could be a useful biomarker in predicti ... | 2013 | 23659555 |
| engagement of the atr-dependent dna damage response at the human papillomavirus 18 replication centers during the initial amplification. | we have previously demonstrated that the human papillomavirus (hpv) genome replicates effectively in u2os cells after transfection using electroporation. the transient extrachromosomal replication, stable maintenance, and late amplification of the viral genome could be studied for high- and low-risk mucosal and cutaneous papillomaviruses. recent findings indicate that the cellular dna damage response (ddr) is activated during the hpv life cycle and that the viral replication protein e1 might pla ... | 2013 | 23135710 |
| human papillomavirus 18 e1^e4 protein interacts with cyclin a/cdk 2 through an rxl motif. | the human papilloma virus e4 protein is highly expressed in late times of infection. evidence to date suggests that e4 is essential for amplification of the viral genome and that it can influence cell cycle. examination of the sequences encoding the e4 proteins from several genotypes of human papillomavirus revealed the presence of rxl-containing motifs reminiscent of the cyclin-binding motifs that have been identified in several cyclin-binding proteins. when baculovirus-produced human cyclin e ... | 2013 | 23065011 |
| mediator probe pcr: a novel approach for detection of real-time pcr based on label-free primary probes and standardized secondary universal fluorogenic reporters. | the majority of established techniques for monitoring real-time pcr amplification involve individual target-specific fluorogenic probes. for analysis of numerous different targets the synthesis of these probes contributes to the overall cost during assay development. sequence-dependent universal detection techniques overcome this drawback but are prone to detection of unspecific amplification products. we developed the mediator probe pcr as a solution to these problems. | 2012 | 22923452 |
| anal canal neuroendocrine carcinoma associated with squamous intraepithelial neoplasia: a human papillomavirus 18-related lesion. | neuroendocrine carcinoma (nec) of the anal canal is exceedingly rare and its histogenesis is poorly understood. we present a case of small-cell nec of the anal canal in a 70-year-old woman. the nec appeared as a submucosal tumor at the dentate line and was associated with squamous intraepithelial neoplasia (sin). the nec was positive for neuroendocrine markers including synaptophysin, chromogranin a and cd56, whereas the sin component did not express any of these markers. both components exhibit ... | 2012 | 22524667 |
| clear cell adenosquamous carcinoma of the cervix: a case report with discussion of the differential diagnosis. | clear cell adenosquamous carcinoma is an extremely rare and recently described variant of adenosquamous carcinoma of the cervix. it has a unique histologic appearance and has been shown to be associated with human papillomavirus-18. its significance lies in the fact that it is associated with an aggressive clinical course and has to be differentiated microscopically from clear cell carcinoma and glassy cell carcinoma. | 2012 | 22498949 |
| effect of sinecatechins on hpv-activated cell growth and induction of apoptosis. | green tea catechins possess a wide range of pharmacological properties, including antiviral, anti-infective, and immunostimulatory properties. they also have demonstrated inhibitory effects on a variety of enzymatic and metabolic pathways involved in cancer development. catechins have been shown to have antiproliferative properties in various cell lines and may have direct virucidal effect. the united states food and drug administration has approved a topical ointment formulation of sinecatechin ... | 2012 | 22468171 |
| site-directed mutagenesis of human papillomavirus 18 promoter elements and tissue-specific expression in cervical carcinoma cells. | in this study, the effect of mutations in transcription factor-binding elements was investigated in the human papillomavirus (hpv) 18 p(105) promoter. site-directed mutagenesis activities, in the ap1/yy1-, krf-1-, gre/yy1-, sp1- and the double mutation (ap1/yy1- and gre/yy1)-binding sites were assessed in five human cell lines: hela (hpv18-positive cervical carcinoma), siha (hpv16-positive cervical carcinoma), c33a (hpv-negative cervical carcinoma), h1299 (non-small cell lung carcinoma) and mrc- ... | 2012 | 22350992 |