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isolation of cdna and enzymatic properties of betaine aldehyde dehydrogenase from zoysia tenuifolia.we isolated cdnas encoding betaine aldehyde dehydrogenase (badh, ec 1.2.1.8) from the salt-tolerant poaceae, zoysia tenuifolia by polymerase chain reactions. zoysia betaine aldehyde dehydrogenase 1 (zbd1) is 1892bp long and codes for 507 amino acids. the deduced amino acid sequence of zbd1 is 88% similar to the sequence of rice badh. ten cdna clones were isolated from a cdna library of salt-treated z. tenuifolia by using the zbd1 fragment as a probe. the proteins coded in some clones were more h ...200516255165
mechanism of action of arenesulfonylhydrazones of 2-pyridinecarboxaldehyde 1-oxide in l1210 cells.the arenesulfonylhydrazones of 2-pyridinecarboxaldehyde 1-oxide represent a relatively new class of anticancer agents. the biochemical alterations responsible for antineoplastic activity were investigated using the most potent member of this class synthesized to date, the 3,4-dimethoxybenzenesulfonylhydrazone of 2-pyridinecarboxaldehyde 1-oxide (3,4-dsp), as the prototype compound. the primary biochemical lesion observed was the production of dna single-strand breaks, which were analyzed using a ...19846498834
[sewage sludge as fertilizer for grassland].the experiment of sewage sludge composted as fertilizer for zoysia japonica was conducted, and the results showed that cd content in soil was lower than 0.3 mg.kg-1 under the condition of the fertilizer application lower than 45 t.hm-2 (dry weight) and the ph value of soil lower than 6.5 (cd as the limiting factor for the amount of fertilizer application). no3(-)-n content of groundwater was lower than the standard of the first class groundwater (10 mg.kg-1). the contents of organic matter, avai ...200212222055
use of amnion and placenta in neonatal screening for canine gm1-gangliosidosis and the risk of diagnostic misclassifications.a closed breeding colony of shiba dogs with gm1-gangliosidosis is maintained at hokkaido university (sapporo, japan). neonatal genotyping is essential to control the breeding colony genetically as an animal model for the human disease.200616511797
[chemical forms of heavy metals in the soils and plants of copper tailings yard].the study on the chemical forms of heavy metals in the soils and plants at the copper tailings yard in tongling city indicated that the soils were extremely poor, with organic mater content being 2.6-5.8 g x kg(-1), only 1/15 of the control, while their cu, cd, pb and zn contents were higher than the control, with cu 809.30-1395.54 mg x kg(-1) and cd 3.25-6.35 mg x kg(-1), as 30-60 times as those of the control. the heavy metals contents in zoysia japonica and trifolium pratense had a significan ...200516515200
transgenic zoysiagrass (zoysia japonica) plants obtained by agrobacterium-mediated transformation.zoysiagrass (zoysia japonica steud.) is an important turfgrass that spreads by stolons and rhizomes. by exploring the potential of direct shoot formation from stolons, we developed a straightforward and efficient transformation protocol without callus induction and propagation. sterilized stolon nodes were infected and co-cultivated with agrobacterium tumefaciens harboring pcambia vectors. hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the s ...200616523287
fasting-induced suppression of pulsatile luteinizing hormone secretion is related to body energy status in ovariectomized goats.the effect of energy status on the response of luteinizing hormone (lh) pulse frequency to acute short-term energy deficiency created by fasting in estradiol-treated ovariectomized shiba goats was studied in two experiments. in experiment 1, eight goats whose mean body weight (bw) was 25.6 +/- 5.8 (mean +/- s.d.)kg were fed 500 g hay cubes daily for 1 week. then they were fasted for 3 days. blood samples were collected for 4 h at 6 min intervals on the last day of feeding, first, second and thir ...200212137981
contribution of rpob2 rna polymerase beta subunit gene to rifampin resistance in nocardia species.nocardia species are gram-positive environmental saprophytes, but some cause the infectious disease nocardiosis. the complete genomic sequence of nocardia farcinica ifm 10152 has been determined, and analyses indicated the presence of two different rna polymerase beta subunit genes, rpob and rpob2, in the genome (j. ishikawa, a. yamashita, y. mikami, y. hoshino, h. kurita, k. hotta, t. shiba, and m. hattori, proc. natl. acad. sci. usa 101:14925-14930, 2004). these genes share 88.8% identity at t ...200616569850
a model of spontaneous periodontitis in the miniature goat.the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis.200616671878
ultrastructural localization of hyaluronic acid in the synovium of the goat knee joint.in the japanese miniature (shiba) goat, the synovial membrane contains synoviocytes referred to as type a (macrophage-like cells) and type b cells (fibroblast-like cells) in the intimal layer. small capillaries and blood vessels of varying sizes were located in the extracellular matrix in the synovial subintima. the type a cells in the synovium possessed numerous vesicles, vacuoles and lysosomes as well as pinocytotic vesicles. these ultrastructural features indicating phagocytosis showed distin ...200415481846
incidence of canine glaucoma with goniodysplasia in japan : a retrospective study.the incidence of primary and secondary glaucoma in dogs was investigated. a total of 1244 dogs received ophthalmologic examinations, including tonometry and gonioscopy. goniophotographs were taken using a goniolens to evaluate the iridocorneal angle (ica) as well as pectinate ligament (pl). the anterior width of the ciliary cleft and the total distance from the origin of the pl to the anterior corneal surface were measured from the goniophotographs. glaucoma was diagnosed based on the cupping of ...200616953087
immunolocalization of nerve growth factor (ngf) and its receptors (trka and p75lngfr) in the reproductive organs of shiba goats.the objective of this study was to determine the immunolocalization of ngf and its receptors (trka and p75lngfr) in the reproductive tract of the japanese shiba goats. five adult goats were used in this study and sections of ovaries, uteri and oviducts were immunostained by the avidin-biotin-peroxidase complex method (abc). the results showed that ngf and its receptors (trka and p75lngfr) were expressed in granulosa cells, theca cells, interstitial cells and lutein cells in ovaries. immunoreacti ...200515738622
prognostic factors associated with survival in dogs with lymphocytic-plasmacytic enteritis.prognostic factors associated with survival in dogs with lymphocytic-plasmacytic enteritis (lpe) were investigated through a retrospective study. using case records, 48 dogs diagnosed with lpe were classified as survivors (n = 32) or non-survivors (n = 16), and the clinical and clinicopathological parameters were reviewed between the 2 groups by using univariate and multivariate prognostic analysis. compared to the hospital population, non-survivors had an overrepresentation of the shiba breed. ...200617019061
allelic variation of the d4 dopamine receptor polymorphic region in two dog breeds, golden retriever and shiba.the d4 dopamine receptor (d4dr) polymorphic region, which is possibly related to the personality trait known as novelty seeking in humans, was examined in 34 dogs from two breeds (golden retriever and the japanese indigenous breed, shiba) by the polymerase chain reaction (pcr), and the dna sequences of each allele were determined. the polymorphic region of the dog d4dr gene was composed of 39- and 12- base pair (bp) units, and four alleles (a-d) were identified based on the number and/or order o ...199910651047
impact of japanese beetle (coleoptera: scarabaeidae) feeding on seashore paspalum.ten cultivars of seashore paspalum, paspalum vaginatum swartz, were compared for their response to japanese beetle, popillia japonica newman, larval root feeding. cultivars of bermuda grass, cynodon sp., and zoysiagrass, zoysia sp., also were included for comparison. turf grown in pots in the greenhouse was infested with second and third instars in this 2-yr study. grub survival and weight gain, foliar growth, and root loss were compared among turfgrass species and cultivars. few species-related ...200617066801
systemic candidiasis in a dog, developing spondylitis.a 4-year-old male shiba dog initially presented with pain of an undetermined origin and hypersensitivity to touch. seven days later, the dog developed ataxia, hind-leg weakness and knuckling. the dog died on 20 days after presentation. postmortem examination revealed a mass in the body of thoracic vertebra. histopathologically, the mass consisted of granulomatous inflammation, including fungal organisms that were immunohistochemically positive for candida albicans. similar granulomatous lesions ...200617085895
flavonoids of zoysiagrass (zoysia spp.) cultivars varying in fall armyworm (spodoptera frugiperda) resistance.flavonoid profiles of 12 zoysiagrass (zoysia spp.) cultivars sampled six times in 1998 were correlated to fall armyworm (spodoptera frugiperda j. e. smith) larval weights and survival on replicated field-grown plant material and analyzed to determine genetic and seasonal variations of flavonoids among zoysiagrass cultivars. from multiple regression analyses and correlations, flavonoid peak 10 (luteolin-glucoside) had the greatest positive association with average fall armyworm weight; however, r ...200717288435
differential expression pattern of c4 bundle sheath expression genes in rice, a c3 plant.nadp-malic enzyme (nadp-me) and phosphoenolpyruvate carboxykinase (pck) are specifically expressed in bundle sheath cells (bscs) in nadp-me-type and pck-type c4 plants, respectively. unlike the high activities of these enzymes in the green leaves of c4 plants, their low activities have been detected in the leaves of c3 plants. in order to elucidate the differences in the gene expression system between c3 and c4 plants, we have produced chimeric constructs with the beta-glucuronidase (gus) report ...200515753103
carbaryl, 2,4-d, and triclopyr adsorption in thatch-soil ecosystems.thatch development in intensively managed turf sites may cause environmental concerns for greater sorption or leaching of applied chemicals in terrestrial ecosystems. to determine the adsorption potential of carbaryl (1-napthyl n-methylcarbamate), 2,4-d (2,4-dichloro-phenoxyacetic acid), and triclopyr (3,5,6-trichloro-2-pyridinyloxyacetic acid) in turf ecosystems, composite thatch and underlying soil samples from three-and six-year-old stands of cool-season southshore creeping bentgrass (agrosti ...200516190014
isolation, characterization and mapping of simple sequence repeat markers in zoysiagrass (zoysia spp.).the genus zoysia consists of 16 species that are naturally distributed on sea coasts and grasslands around the pacific. of these, zoysia japonica, zoysia matrella, and zoysia tenuifolia are grown extensively as turfgrasses, and z. japonica is also used as forage grass in japan and other countries in east asia. to develop simple sequence repeat (ssr) markers for zoysiagrass (zoysia spp.), we used four ssr-enriched genomic libraries to isolate 1,163 unique ssr clones. all four libraries contained ...200516235048
environmental risk assessment of genetically engineered herbicide-tolerant zoysia japonica.herbicide-tolerant zoysia grass (zoysia japonica steud.) has been generated previously through agrobacterium tumefaciens-mediated transformation. the genetically modified (gm) zoysia grass survived basta spraying and grew to maturity normally while the wild-type (wt) grass stopped growing and died. gm zoysia grass will permit more efficient weed control for various turf grass plantings such as home lawns, golf courses, and parks. we examined the environmental/biodiversity risks of herbicide-tole ...200818178894
molecular screening of canine gm1 gangliosidosis using blood smear specimens after prolonged storage: detection of carriers among shiba dogs in northern japan.molecular screening of gm1 gangliosidosis in shiba dogs was carried out in northern japan using blood smear specimens after prolonged storage. of 125 specimens obtained from 3 veterinary teaching hospitals for this screening, 68 specimens (54%) were adequate for direct amplification in a polymerase chain reaction (pcr)-based dna test, and the percentage of adequacy was different at each hospital (34%, 73%, and 100%), suggesting that the amount of blood on the smear and the storage condition of s ...200818182512
establishment of feeder-independent cloned caprine trophoblast cell line which expresses placental lactogen and interferon tau.a feeder-independent cloned trophoblast cell line, hts-1, was established from a mature placenta of shiba goat (capra hircus). during the growth phase, single hts-1 cells exhibited ruffled membranes or lamellipodia often accompanied by elongated cell shape, indicating highly motile nature of the cells. at or near confluence, hts-1 cells formed monolayers with few sign of cellular overlapping. binucleate cells were found at a high frequency especially in the peripheral regions of monolayers. in s ...200212361681
[dispensing drugs without prescriptions and the separation of drug dispensaries from the medical practice].many cases of dispensing drugs without doctors' prescriptions were reported throughout japan during 1914-16. some cases were brought to the courts. pharmacists had been selling prescription drugs to their customers without prescriptions. this dispensing act was admitted by the ministry of internal affairs after 1913. however, doctors claimed that the act of dispensing drugs without a prescription infringed on law no. 10, and the japan pharmacists association hired a notable lawyer and fought a l ...200212412593
levels of tolerance, antibiosis, and antixenosis among resistant buffalograsses and zoysiagrasses.the western chinch bug, blissus occiduus barber, has been documented as one of the most serious pests of buffalograss, buchloë dactyloides (nuttall) engelmann, and zoysiagrass, zoysia japonica steudel, grown for turf in midwestern states. resistance to the western chinch bug has been identified in both buffalograsses and zoysiagrasses. choice and no-choice studies were conducted to determine the categories (antibiosis, antixenosis, and tolerance) of three resistant buffalograsses (px3-5-1', 196' ...200818459421
hormonal control of grooming behavior in domestic goats.in this study, further documentation of the modulation of self-grooming by testosterone was provided by an experiment which showed that self oral grooming in long-term castrated male goats of the dwarf shiba breed was significantly down-regulated to one-third of the baseline grooming rate by testosterone supplementation. observations on grooming in gonadally intact female shiba goats in the summer (july) and in the fall (october) showed that the oral grooming rate was significantly higher in the ...200312536011
turfgrass thatch effects on pesticide leaching: a laboratory and modeling study.process-based models are frequently used to assess the water quality impacts of turfgrass management emanating from proposed or existing golf courses. thatch complicates the prediction of pesticide transport because surface-applied pesticides must pass through an organic-rich layer before entering the soil. this study was conducted to (i) compare the use of a linear equilibrium model (lem) and two-site nonequilibrium (2sne) model to predict pesticide transport through soil and thatch + soil colu ...200312549561
morphological analysis of corneal opacity in shiba dog with gm1 gangliosidosis.gm1 gangliosidosis is one of the inherited metabolic lysosomal storage disorders characterized by neurological symptoms caused by beta-galactosidase deficiency and consequent accumulation of gm1 ganglioside in neuronal cells. shiba dogs affected with gm1 gangliosidosis have been found to suffer from corneal opacity. in our morphological analysis, keratocyte enlargement was induced by abnormal intracellular accumulation of neutral carbohydrates, resulting in the loss of normal arrangement of coll ...200818840960
different effects of subnormal levels of progesterone on the pulsatile and surge mode secretion of luteinizing hormone in ovariectomized goats.this study tested the hypothesis that endocrinological threshold levels of progesterone that induce negative feedback effects on the pulsatile and surge modes of lh secretion are different. our approach was to examine the effects of subnormal progesterone concentrations on lh secretion. long-term ovariectomized shiba goats that had received implants of silastic capsules containing estradiol were divided into three groups. the high progesterone (high p) group received a subcutaneous implant of a ...200312606364
induction of systemic resistance in arabidopsis thaliana in response to a culture filtrate from a plant growth-promoting fungus, phoma sp. gs8-3.the plant growth-promoting fungus (pgpf), phoma sp. gs8-3, isolated from a zoysia grass rhizosphere, is capable of protecting cucumber plants against virulent pathogens. this fungus was investigated in terms of the underlying mechanisms and ability to elicit systemic resistance in arabidopsis thaliana. root treatment of arabidopsis plants with a culture filtrate (cf) from phoma sp. gs8-3 elicited systemic resistance against the bacterial speck pathogen pseudomonas syringae pv. tomato dc3000 (pst ...200919121119
modulation of gonadotrophin-releasing hormone pulse generator activity by the pheromone in small ruminants.in small ruminants, such as goats and sheep, a primer pheromone produced by males induces an out-of-seasonal ovulation in anoestrous females, a phenomenon known as the male effect. the male effect is unique in that an external chemical stimulus can immediately modulate the activity of the hypothalamic gonadotrophin-releasing hormone (gnrh) pulse generator. we have established a monitoring method of the gnrh pulse generator activity in shiba goat. using this method as a sensitive bioassay to asse ...200919207811
[effects of land utilization of sewage sludge on grass and soils].effects of land disposal of sewage sludge on grass and soil environment were studied. the sewage sludge used was from northern shenyang wastewater treatment plant. the results showed that contents of nutrient in the soil were increased after sewage sludge application, especially for organic matter. grass biomass were increased and the green period were extended with a better growth of the lawn. the heavy metal contents in the soil were increased with cd contents beyond 2nd grade national environ ...200312800658
responses of gonadotropin secretion to short-term dietary supplementation in ovariectomized goats with different body weights.the responses of luteinizing hormone (lh) and follicle stimulating hormone (fsh) secretion to acute dietary supplementation were studied in goats with different body weights. ovariectomized shiba goats (n=11) were used and were maintained with a feed of 100% of their energy requirement. they were implanted subcutaneously with an oestradiol capsule and were divided into light (lbw; <or=24kg, n=6, mean+/-s.d., 21.8+/-2.7kg) and heavy body weight (hbw; >24kg, n=5, mean+/-s.d., 32.0+/-6.3kg) groups ...200919268503
collagenofibrotic glomerulonephropathy with fibronectin deposition in a dog.we report herein a case of collagenofibrotic glomerulonephropathy in a 3-year-old shiba inu with severe proteinuria. histologically, renal glomeruli were enlarged with massive deposition of a homogeneous eosinophilic substance within the mesangium and capillary walls. the deposits reacted weakly with periodic acid-schiff, stained deep blue with masson's trichrome, and were positive by immunofluorescence for type iii collagen and fibronectin. ultrastructurally, the deposits consisted of fibrils a ...200919276051
further details and sem observations on meloidogyne marylandi (nematoda: meloidogynidae).specimens of meloidogyne marylandi from bermuda grass and a population from zoysia grass were examined and compared morphologically by light and electron microscopy. the populations probably are conspecific and the differences noted in the zoysia population, mainly those of second-stage juveniles (j2) with shorter tails, are considered normal variations rather than representing another form. scanning electron microscope observations provided additional details of the perineal pattern and head of ...198919287638
description and sem observations of dolichodorus marylandicus n. sp. with a key to species of dolichodorus.dolichodorus marylandicus n. sp. is described and illustrated from grass (zoysia japonica) in college park, maryland. specimens have also been collected from perennial bluegrass (poa pratensis) pasture at beltsville, maryland, and from pine (pinus sp.) in north carolina. this new species is related to d. heterocephalus cobb, d. similis golden, and d. aestuarius chow &taylor. females are distinct in having a longer styler and shorter body length than d. aestuarius. the excretory pore is at the le ...198119300733
[studies on parafossarulus manchouricus bourguigant in korea]a snail of the primary host of clonorchis sinensis, distributed in the korean penninsula, has been called bulimus striatulus (benson) shiba 1934, while that of china, the mainland of asia, and of japan has been called parafossarulus manchouricus bourguigant(abbott l951, sugihara l954). having studied on the external character and radula of the snail collected from nine areas of korea, including kimhae, jinyong, namji, ulsan, kunsan etc.the author has not found any reasonable distinctions which s ...196412913606
drought stress increases the production of 5-hydroxynorvaline in two c4 grasses.plants produce various compounds in response to water deficit. here, the presence and identification of a drought-inducible non-protein amino acid in the leaves of two c(4) grasses is first reported. the soluble amino acids extracted from the leaves of three different species were measured by high-performance liquid chromatography of derivatives formed with o-phthaldialdehyde and beta-mercaptoethanol. one amino acid that increased in amount with drought stress had a retention time not correspond ...200919359022
association analysis between canine behavioural traits and genetic polymorphisms in the shiba inu breed.the relationships between behavioural trait data and the genotype of 15 polymorphisms in eight neurotransmitter-related genes were analysed in 77 dogs of the shiba inu breed, an indigenous japanese dog. the data were obtained from a 26-item questionnaire on the dog's behaviour, distributed to the dog's owners, through veterinary hospitals and the shiba inu breed magazine. a factor analysis of the questionnaire items extracted eight factors accounting for 66.8% of the variance. an association ana ...200919397510
host preference of the chinch bug, blissus occiduus.the chinch bug, blissus occiduus barber (hemiptera: blissidae), is an important pest of buffalograss, buchloë dactyloides (nutall) engelmann and potentially other turfgrass, crop, and non-crop hosts. choice studies documented the number of b. occiduus present on selected turfgrasses, crops and weeds, and provided important insights into the host preferences of this chinch bug. grasses with the most chinch bugs present included the warm-season turfgrasses b. dactyloides , zoysiagrass, zoysia japo ...200619537992
surgical correction of cor triatriatum dexter in a dog under extracorporeal circulation.a shiba inu dog with marked abdominal distension was diagnosed with cor triatriatum dexter and surgical correction was performed under extracorporeal circulation. the total duration of cardiac arrest was 11 minutes and total perfusion time was 34 minutes. the dog had an uneventful postoperative recovery. postoperative contrast radiography of the caudal vena cava revealed normal flow into the right heart. abdominal distension was no longer observed. although several methods have been used to trea ...200312934813
production of herbicide-tolerant zoysiagrass by agrobacterium-mediated transformation.herbicide-resistant zoysiagrass (zoysia japonica steud.) has been developed by agrobacterium-mediated transformation. a callus-type transformation system was established by optimizing several factors that affect the rate of transformation, including co-cultivation period and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d), cacl2 and acetosyringone. maximal gus expression was observed when a type 3 callus was co-cultivated on 2,4-d-free co-cultivation medium for 9 d. in addition, removal ...200314503840
inspiratory activation of the vocal cord adductor, part ii: animal study in the cat.the authors have shown previously that the vocal cord adductor is activated during inspiration in patients with vocal cord abduction impairment and that this adductor inspiratory activity is abolished by relief from inspiratory tracheal negative pressure by opening the tracheostoma. (shiba k. isono s, sekita y, tanaka a. inspiratory activation of the vocal cord adductor, part i: human study in patients with restricted abduction of the vocal cords. laryngoscope 2004;114:372-375). the authors hypo ...200414755222
histochemistry of complex carbohydrates in the ceruminous glands of the goat.the localization and chemical nature of complex carbohydrates in the ceruminous glands of the japanese miniature (shiba) goat were studied using light and electron microscopic histochemical methods, particularly lectin histochemistry. the epithelial cells and luminal secretion of the caprine ceruminous glands contained large amounts of neutral and smaller amounts of acidic glycoconjugates with different terminal sugars (alpha- d-mannose, alpha-l-fucose, alpha-n-acetyl-d-galactosamine, beta-d-gal ...200415083309
increased concentration of gm1-ganglioside in cerebrospinal fluid in dogs with gm1- and gm2-gangliosidoses and its clinical application for diagnosis.gm1- and gm2-gangliosidoses are lethal lysosomal diseases that are caused by a defect of acid hydrolases, resulting in the intralysosomal accumulation of the specific physiological substrates, gm1- and gm2-gangliosides, respectively. in the present study a method for the diagnosis of canine gm1-gangliosidosis was established using canine cerebrospinal fluid (csf). the concentration of gm1-ganglioside in csf was determined by thin-layer chromatography-enzyme immunostaining using biotin-conjugated ...200415152837
ovipositional preferences of the japanese beetle (coleoptera: scarabaeidae) among warm- and cool-season turfgrass species.japanese beetles, popillia japonica newman (coleoptera: scarabaeidae), were evaluated for ovipositional preferences among four turfgrasses common in northwestern arkansas. choice assays revealed females preferred to oviposit in tall fescue (festuca arundinacea schreb.) and zoysiagrass (zoysia japonica steud.), and that they avoided oviposition in common bermudagrass (cynodon dactylon [l.] pers.) and hybrid bermudagrass (c. dactylon x c. transvaalensis pers.). significantly fewer eggs were ovipos ...200920069848
postnatal development of multivesicular nuclear body in the shiba goat sertoli cell: an ultrastructural study.the multivesicular nuclear body (mnb) within the sertoli cell nucleolus has been observed in the ruminant testis, but not in other mammalian species. generally, the mnb is composed of vesicles, tubules and ribosome-like structures. this study has been conducted in order to clarify mnb formation during postnatal development. the testes were obtained from immature shiba goats at 1, 2, 3, 4, and 5 months old, and from adults. they were fixed with 5% glutaraldehyde, postfixed with 1% oso4, dehydrate ...200415248561
rapid and reliable genotyping technique for gm1 gangliosidosis in shiba dogs by real-time polymerase chain reaction with taqman minor groove binder probes.real-time polymerase chain reaction (pcr) with taqman minor groove binder (mgb) probes was examined to establish a rapid and reliable genotyping technique for gm1 gangliosidosis in shiba dogs. this technique was applied to dna samples extracted from the blood, umbilical cord, or postmortem liver tissue specimens, and to dna-containing solutions prepared from blood and saliva that had been applied to flinders technology associates filter papers (fta cards). the amplification of the targeted seque ...201020224082
early pregnancy diagnosis by means of ultrasonography as a method of improving reproductive efficiency in goats.eighteen cyclic shiba goats were used in this study. estrus was synchronized with a single injection of 125 microg of a synthetic analogue of prostaglandin f(2)alpha (pgf(2)alpha) after detection of at least one corpus luteum by b-mode ultrasonography. blood samples were collected from each animal on days 0, 7 and 21 post-mating for progesterone assay. animals in estrus were either allowed to be mated by fertile bucks twice during estrus (group i; n=12) or not at all (group ii; n=6). ultrasonogr ...200415329470
an ultrastructural study on cytotoxic effects of mono(2-ethylhexyl) phthalate (mehp) on testes in shiba goat in vitro.in this study, the effects of mono(2-ethylhexyl) phthalate (mehp), one of metabolites of di(2-ethylhexyl) phthalate, on immature shiba goat testes in vitro were examined. the testes of 2-month-old shiba goats were cut into smaller pieces, and seeded in medium. at 1, 3, 6 and 9 hr after administration of mehp at various concentrations (0, 100 nmol ml(-1), 1 nmol ml(-1), and 1 x 10(-3) nmol ml(-1), respectively), the specimens were obtained for light and transmission electron microscopic observati ...200415365238
male effect pheromone tickles the gonadotrophin-releasing hormone pulse generator.in sheep and goats, the primer pheromone produced by the male induces out-of-seasonal ovulation in anoestrous females, the so-called 'male effect.' because the initial endocrine event following reception of the pheromone is the stimulation of pulsatile luteinising hormone (lh) secretion, the central target of the pheromone is considered to be the putative gonadotrophin-releasing hormone (gnrh) pulse generator. using electrophysiological techniques to record multiple-unit activity (mua) in close ...201020646176
does exogenous progesterone and oestradiol treatment from the mid-luteal phase induce follicular cysts in goats?the purpose of the present study was to investigate the effects of exogenous ovarian steroid treatment, which is known to induce follicular cyst experimentally in cows, on ovarian activity in goats. eleven female shiba goats with the length of the normal oestrous cycle (approximately 21 days) received subcutaneously either 1 ml of ethanol (control group, n=4) or 4 mg of progesterone and 2mg of oestradiol (treatment group, n=7) daily for 7 days beginning on day 14 of the oestrous cycle (day 0=ovu ...200716542801
surgical stabilization of an occipitoatlantoaxial malformation in an adult dog.to report surgical planning, technique, and outcome of stabilization surgery in an adult dog with occipitoatlantoaxial malformation (oaam).201020880141
prediction of the external work of the native heart from the dynamic h-q curves of the rotary blood pumps during left heart bypass.the ventricular performance is dependent on the drainage effect of rotary blood pumps (rbps) and the performance of rbps is affected by the ventricular pulsation. in this study, the interaction between the ventricle and rbps was examined using the pressure-volume (p-v) diagram of the ventricle and dynamic head pressure-bypass flow (h-q) curves (h, head pressure: arterial pressure minus ventricular pressure vs. q, bypass flow) of the rbps. we first investigated the relationships in a mock loop wi ...201020883395
secretion of progesterone during long and short days of the estrous cycle in goats that are continuous breeders.this study was conducted 1) to determine if the secretion of progesterone, as an index of ovarian activity, during the estrous cycle of nonseasonal shiba goats is affected by seasonal changes, and 2) to learn if the pulsatile secretion of ovarian progesterone can be estimated from samples obtained by cannulation into the caudal vena cava via the femoral vein. progesterone concentrations in jugular venous plasma during the estrous cycle in spring (may) were similar to those in autumn (november). ...199516727669
ovarian expression of inhibin-subunits, 3β-hydroxysteroid dehydrogenase, and cytochrome p450 aromatase during the estrous cycle and pregnancy of shiba goats (capra hircus).the cellular localization of the inhibin subunits (α, β(a), and β (b)), steroidogenic enzymes (3β-hydroxysteroid dehydrogenase (3βhsd) and cytochrome p450 aromatase (p450arom) were evaluated in the ovaries of cyclic (n=6) and pregnant (n=2) shiba goats (capra hircus). the immunointensity of inhibin α and β(a) subunits showed an increase in the granulosa cells (gc) of developing follicles. inhibin β(b) subunit and p450arom showed high expression in gc of antral follicles. 3βhsd immunoreactivity w ...201021030788
histochemistry of sialoglycoconjugates in goat submandibular glands.with 8 figures and 3 tables summary: the functional properties of sialic acids appear to be manifold. therefore, they are considered as essential components of saliva. in this study, the localization of sialoglycoconjugates in the submandibular glands of japanese miniature (shiba) goat was examined by light and electron microscopic histochemical methods. the submandibular glands exhibited a large amount of sialic acids. additionally, sialic acids with o-acetyl substitutions were detectable in th ...201021175740
effects of estrogen on growth hormone pulsatility in peripheral blood and neuropeptide profiles in the cerebrospinal fluid of goats.we previously reported that growth hormone (gh) pulses were negatively associated with neuropeptide y (npy) profiles in cerebrospinal fluid (csf) of the third ventricle of shiba goats. in addition, while most gh pulses were coincident with gh-releasing hormone (ghrh) pulses, there was no correlation between gh and somatostatin (srif) levels. the present study was performed to elucidate the relationship between gh pulses and these neuropeptide levels in csf when estradiol (1.0 mg/head) was subcut ...201121242654
severe hypertriglyceridemia influenced by alcohol (shiba).this study was conducted to examine the relationship between triglyceride (tg) levels and a history of excessive drinking in patients with severe hypertriglyceridemia (ht).201121245063
myogenic and adipogenic properties of goat skeletal muscle stem cells.the aims of the present study were to establish a culture system for goat skeletal muscle stem cells and to examine their myogenic and adipogenic properties in vitro. cells were isolated from the skeletal muscle of the shiba goat and cultured in vitro. most of the cells were positive for myogenic markers, such as pax7, myod, and desmin, and immunocytochemistry revealed they differentiated to form myotubes expressing myosin heavy chain, indicating they were highly myogenic. myogenic differentiati ...200717008759
changes in the ovarian dynamics and endocrine profiles in goats treated with a progesterone antagonist during the early luteal phase of the estrous cycle.the aim of the present study was to determine the physiological role of endogenous progesterone in the regulation of ovarian dynamics, gonadotropin and progesterone secretion during the early luteal phase in the goat. cycling shiba goats received subcutaneously a vehicle (control group, n=5) or 50 mg of ru486 (ru486 group, n=4) daily from 1 to 7 days after ovulation (day 0) determined by transrectal ultrasonography. ovarian dynamics were monitored by the ultrasonography and blood samples were co ...200717027203
apparent pseudohyperkalemia in a chinese shar pei dog.whole blood in a serum clot tube and edta-anticoagulated samples from an 8-year-old spayed female chinese shar pei dog were submitted by an external clinic to the diagnostic laboratory at atlantic veterinary college for routine biochemical and hematologic analysis prior to entropion surgery. laboratory abnormalities included mild hyperkalemia (6.3 mmol/l, reference interval 3.6-6.0 mmol/l), mild normocytic, hypochromic, nonregenerative anemia (hct 0.31 l/l, reference interval 0.37-0.55 l/l; mchc ...200717311201
congenital oesophageal stricture in a japanese shiba inu.an 11-week-old, female, japanese shiba inu, weighing 1.3 kg, was presented with a history of intermittent regurgitation since birth. an oesophagram showed a partial obstruction of the caudal cervical oesophagus associated with a narrow, transverse tissue band. following surgical resection, the regurgitation resolved and the dog attained a normal bodyweight. histological examination revealed focally hyperplastic epithelium with dense fibrous underlying connective tissue forming the band. the diag ...200717725590
cloning of canine myocilin cdna and molecular analysis of the myocilin gene in shiba inu dogs.to identify canine myocilin cdna and compare its sequence in glaucomatous and nonglaucomatous shiba inu dogs with closed and open iridocorneal angles (icas).200717973835
photorespiration in c4 grasses remains slow under drought conditions.the co(2)-concentrating mechanism present in c(4) plants decreases the oxygenase activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) and, consequently, photorespiratory rates in air. under drought conditions, the intercellular co(2) concentration may decrease and cause photorespiration to increase. the c(4) grasses paspalum dilatatum poiret, cynodon dactylon (l.) pers. and zoysia japonica steudel were grown in soil and drought was imposed by ceasing to provide water. net co(2) ...200818331589
immunolocalization of steroidogenic enzymes in the fetal, neonatal and adult testis of the shiba goat.the localizations of steroidogenic enzymes (p450scc, 3 beta hsd, p450c17 and p450arom) in testes of shiba goats were investigated by immunohistochemistry. p450scc, 3 beta hsd, p450c17 and p450arom were detected in all leydig cells of adults. p450scc and p450c17 were observed in most leydig cells in the fetus (90 days) and neonate (15 days). 3 beta hsd and p450arom were found in some leydig cells of the fetus with weak immunostaining but the numbers of immunopositive leydig cells and intense immu ...200516365523
a new chorizococcus species (coccoidea, pseudococcidae) from taiwan with transferring of chorizococcus mirzayansi moghaddam to genus spilococcus ferris.a new mealybug species, chorizococcus zoysiaesp. n., feeding on zoysia tenuifolia (poaceae), is described from taiwan. adult female, third-instar female, second-instar female and first-instar nymph were described and illustrated in this article. keys are provides to (a) separate this new species from similar species of chorizococcus and those of same genus on zoysia grasses and (b) to identify instars of the new species. in addition, chorizococcus mirzayansi moghaddam is transferred to the genus ...201121747678
neutropenia associated with osteomyelitis due to hepatozoon canis infection in a dog.a 4-year-old, intact male shiba dog was referred to yamaguchi university animal medical center, yamaguchi, japan for the following complaints: anorexia, lethargy, intermittent fever, gingival bleeding, and abdominal purpura. the dog presented with persistent neutropenia. histopathological examination of the bone marrow sample revealed round to oval structures that resembled hepatozoon micromerozoites and formed a "wheel-spoke" pattern. furthermore, mature neutrophils were observed around these s ...201121697643
studies on land application of sewage sludge and its limiting factors.field experiments were conducted to study the effect of sewage sludge application on the heavy metal content in soils and grasses. the sewage sludge was obtained from northern shenyang wastewater treatment plant, china, and applied at 0, 15, 30, 60, 120 and 150tha(-1). native grasses zoysia japonica and poa annua were chosen as experimental plants. the experimental results showed that nutrient content of the soil, especially organic matter, was increased after sewage sludge application. the gras ...200818456400
histochemical analyses of glycoconjugates and antimicrobial substances in goat labial glands.saliva is known to protect the oral cavity and contains glycoproteins and antimicrobial substances. the distribution of these salivary secretions was studied in the labial glands of the japanese miniature (shiba) goat using lectin histochemical and immunohistochemical methods. the mucous acinar cells of the labial glands exhibited glycoconjugates with different saccharide residues, such as galnacα1-3galnac, galβ1-4galnac, β-d-glcnac and sialic acid linked to α2-6gal/galnac. furthermore, α-d-man, ...201121925718
relative salinity tolerance of warm season turfgrass species.fresh water, coupled with soil salinization in many areas has resulted in an increased need forscreening of salt tolerant turf grasses. relative salinity tolerance of eightwarm season turfgrass species were examined in this study in sand culture. grasses were grown in a glasshouse, irrigated with either distilled water or saline sea water adjusted to 24, 48 or 72 dsm-1. salt tolerances of the grasses were assessed on the basis of their shoot and root growth, leaf firing and turf quality. regress ...201122167942
Risk factors for canine tail chasing behaviour in Japan.The aim of this study was to investigate the risk factors for tail chasing behaviour that occurs when a dog spins in tight circles to chase its tail, sometimes biting it. The behaviour is a sign of canine compulsive disorder (CD). A questionnaire about tail chasing behaviour and general information about the animals was used to collect data on seven breeds of pet dogs. The data were gathered at a dog event and at veterinary practices. To determine which variables were associated with tail chasin ...201121993593
the activities of pep carboxylase and the c4 acid decarboxylases are little changed by drought stress in three c4 grasses of different subtypes.the c4 photosynthetic pathway involves the assimilation of co2 by phosphoenolpyruvate carboxylase (pepc) and the subsequent decarboxylation of c4 acids. the enzymes of the co2 concentrating mechanism could be affected under water deficit and limit c4 photosynthesis. three different c4 grasses were submitted to gradually induced drought stress conditions: paspalum dilatatum (nadp-malic enzyme, nadp-me), cynodon dactylon (nad-malic enzyme, nad-me) and zoysia japonica (pep carboxykinase, pepck). mo ...200818629606
a retrospective study of intervertebral disc herniation in dogs in japan: 297 cases.we investigated the epidemiological characteristics of intervertebral disc herniation (ivdh) in japan in a large population using a retrospective study. the sample population was dogs (n=297) with ivdh in japan. medical records were reviewed for breed, sex, age, affected interspace and neurological severity. the dogs were comprised of 132 cases of cervical ivdh (c-ivdh) and 165 cases of thoracolumbar ivdh (tl-ivdh). in japan, the dachshund, beagle and shih tzu tended to suffer from both c-ivdh a ...200818685242
giant charge relaxation resistance in the anderson model.we investigate the dynamical charge response of the anderson model viewed as a quantum rc circuit. applying a low-energy effective fermi liquid theory, a generalized korringa-shiba formula is derived at zero temperature, and the charge relaxation resistance is expressed solely in terms of static susceptibilities which are accessible by bethe ansatz. we identify a giant charge relaxation resistance at intermediate magnetic fields related to the destruction of the kondo singlet. the scaling proper ...201122107549
Pathological Features of Salivary Gland Cysts in a Shiba Dog with GM1 Gangliosidosis: A Possible Misdiagnosis as Malignancy.Salivary gland cysts are often concurrent with GM1 gangliosidosis in Shiba dogs. Although the etiology is unknown, these cysts may be misdiagnosed as malignant due to the accumulation of foamy cells. The present study investigated the cytological, histopathological, immunohistochemical and electron microscopic characteristics of salivary gland cysts in a Shiba dog affected with GM1 gangliosidosis. The salivary gland masses were surgically enucleated and examined clinicopathologically and patholo ...201122083095
possible association of glaucoma with pectinate ligament dysplasia and narrowing of the iridocorneal angle in shiba inu dogs in japan.the purpose of the study was to describe the shape of the pectinate ligament (pl) and to estimate the width of the iridocorneal angle (ica) in glaucomatous and non-glaucomatous eye of shiba inu dogs in japan. one hundred fourteen shiba inu dogs were presented to the veterinary medical center at the university of tokyo between june 1998 and june 2003. among these, 46 dogs had glaucoma; the remaining 68 dogs were presented for routine vaccinations or heartworm testing and were used as a control po ...200616497230
the effects of short-term nutritional stimulus before and after the luteolysis on metabolic status, reproductive hormones and ovarian activity in goats.the effect of short-term nutritional supplementation on hormonal and ovarian dynamics was studied in goats. cycling shiba goats were divided randomly into maintenance (group m, n=4) and high-energy (group h, n=4) groups. after the detection of the ovulation (day 0, 1(st) ovulation), group h received a high-energy diet providing 2.5 times of the maintenance energy requirement for 7 days from day 7 to day 13 and were administered 2 mg of prostaglandin f(2alpha) (pgf(2alpha)) on day 10 to induce lu ...200918957826
billbug (coleoptera: curculionidae) species composition, abundance, seasonal activity, and developmental time in florida.billbugs (coleoptera: curculionidae: sphenophorus spp.) are common pests whose damage is often misdiagnosed on turfgrass in the united states. consequently, florida turfgrass managers have been struggling to satisfactorily control billbug outbreaks. thus, we sought to determine the species complex, abundance, seasonality, and fecundity of key sphenophorus spp. from field collections, and quantify duration of developmental and daily activity periods through greenhouse rearing tests. from january ...200919253650
evaluation of the phytoremediation potential of four plant species for dibenzofuran-contaminated soil.in this experiment, three grasses, bermuda grass (cynodon dactylon), bent grass (agrostis palustris huds.), lawn grass (zoysia japonica), and a shallow-rooted legume, white clover (trifolium repens l.) were planted into uncontaminated soil and dibenzofuran (dbf)-contaminated soil. the germination rates of all plants were investigated using contaminated soils to evaluate their sensitivities to dbf. during 2 months of growth, the root biomass and heterotrophic microbial numbers were measured in or ...200919321258
influence of plant parameters on occurrence and abundance of arthropods in residential turfgrass.the effect of taxa [common bermuda grass, cynodon dactylon (l.); centipedegrass, eremochloa ophiuroides munro hack; st. augustinegrass, stenotaphrum secundatum [walt.] kuntze; and zoysiagrass, zoysia spp.], density, height, and weed density on abundance of natural enemies, and their potential prey were evaluated in residential turf. total predatory heteroptera were most abundant in st. augustinegrass and zoysiagrass and included anthocoridae, lasiochilidae, geocoridae, and miridae. anthocoridae ...200919610427
first case of propionibacterium acnes urinary tract infection in a dog.propionibacterium acnes has been rarely isolated as a commensal from dogs, but there is little evidence of pathogenicity. urinary tract infections are common in dogs and are typically caused by various commensal bacteria. here we present the first case report of a urinary tract infection caused by p. acnes.201526690238
influence of temperature on coherent dynamics of a two-level system immersed in a dissipative spin bath.the quantum dynamics of a two-level system coupled to an ohmic spin bath is studied by means of the perturbation approach based on a unitary transformation. a scattering function xi(k) is introduced in the transformation to take into account quantum fluctuations. by the master equation within the born approximation, nonequilibrium dynamics quantities are calculated. the method works well for the coupling constant 0<alpha<alpha(c) and a finite bare tunneling delta. it is found that (i) only at ze ...200919814562
genome-wide association analysis of canine atopic dermatitis and identification of disease related snps.in humans, genome-wide association studies (gwas) have been shown to be an effective and thorough approach for identifying polymorphisms associated with disease phenotypes. here, we describe the first study to perform a genome-wide association study in canine atopic dermatitis (cad) using the illumina canine snp20 array, containing 22,362 single-nucleotide polymorphisms (snps). the aim of the study was to identify snps associated with cad using affected and unaffected golden retrievers. further ...201019838693
intermittent nutritional stimulus by short-term treatment of high-energy diet promotes ovarian performance together with increases in blood levels of glucose and insulin in cycling goats.the aim of this study was to determine if short-term intermittent treatments of high-energy diet have any stimulatory effects on ovarian function and metabolic status in goat. cycling shiba goats were divided into treatment (tg; n=6) and control (cg; n=6) groups. after the detection of ovulation (1st ovulation, day 0) by ultrasonography, a high-energy diet (250% of maintenance) was fed to the tg from day 12 to day 15 (4 days) and from day 18 to day 21 (4 days). the high-energy diet comprised 100 ...201020961717
echinostoma hortense infection with enteritis diagnosed by upper gastrointestinal endoscopy in a dog.an 8-year-old male shiba dog presented with chronic vomiting and diarrhea. upper gastrointestinal endoscopy revealed severe enteritis and infection of the duodenal mucosa with echinostoma hortense. we performed therapy for parasites and enteritis. the therapy was successful for deworming and temporarily improved the symptoms, but the dog died soon thereafter. to the authors' knowledge, this is the first case report of an antemortem diagnosis of e. hortense infection in a dog.201323449463
isolation, cloning, and characterization of a partial novel aro a gene in common reed (phragmites australis).among the essential amino acids, phenylalanine, tryptophan, and tyrosine are aromatic amino acids which are synthesized by the shikimate pathway in plants and bacteria. herbicide glyphosate can inhibit the biosynthesis of these amino acids. so, identification of the gene tolerant to glyphosate is very important. it has been shown that the common reed or phragmites australis cav. (poaceae) is relatively tolerant to glyphosate.201525474478
functional characterization of the nfxb repressor of the mexcd-oprj multidrug efflux operon of pseudomonas aeruginosa.the mexcd-oprj multidrug efflux operon of pseudomonas aeruginosa is regulated by the nfxb repressor. two forms of nfxb have been reported [shiba et al. (1995). j bacteriol 177, 5872) although mutagenesis studies here confirm that the larger protein (199 amino acids, 22.4 kda) is the functional repressor. nfxb binds upstream of the mexcd-oprj transcription initiation site to a region containing two inverted repeats, both of which are required for binding. two-hybrid assays confirmed that nfxb is ...201323924707
photo-biotechnology as a tool to improve agronomic traits in crops.phytochromes are photosensory phosphoproteins with crucial roles in plant developmental responses to light. functional studies of individual phytochromes have revealed their distinct roles in the plant's life cycle. given the importance of phytochromes in key plant developmental processes, genetically manipulating phytochrome expression offers a promising approach to crop improvement. photo-biotechnology refers to the transgenic expression of phytochrome transgenes or variants of such transgenes ...201525532679
non-markovian dynamics of a biased qubit coupled to a structured bath.a new analytical approach, beyond the rotating wave approximation, based on unitary transformations and the non-markovian master equation for the density operator, is applied to treat the biased spin-boson model with a lorentzian structured bath for arbitrary detunings at zero temperature. compared to zero bias, we find that the non-equilibrium dynamics demonstrates two more damping oscillation frequencies and one additional relaxation frequency for non-zero bias. analytical expressions for the ...201021389459
[study on subsurface wastewater infiltration system covered by different turfgrass for domestic sewage treatment].domestic sewage was treated with subsurface wastewater infiltration system covered by two different turfgrass, namely, festuca arundinacea schres. and zoysia japonica steud.. the result shows that all the different systems have good removal rates to cod. the concentration of cod decreased to less than 48 mg x l(-1) from 97-357 mg x l(-1) which achieve the second class criteria specified in integrated wastewater discharge standard, and there are no prominent difference among different systems. th ...201121404682
effects of field-grown genetically modified zoysia grass on bacterial community structure.herbicide-tolerant zoysia grass has been previously developed through agrobacterium-mediated transformation. we investigated the effects of genetically modified (gm) zoysia grass and the associated herbicide application on bacterial community structure by using culture-independent approaches. to assess the possible horizontal gene transfer (hgt) of transgenic dna to soil microorganisms, total soil dnas were amplified by pcr with two primer sets for the bar and hpt genes, which were introduced in ...201121532315
heterologous expression of a novel zoysia japonica salt-induced glycine-rich rna-binding protein gene, zjgrp, caused salt sensitivity in arabidopsis.a novel zoysia japonica salt-induced glycine-rich rna-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic arabidopsis. glycine-rich rna-binding proteins (grps) play crucial roles in diverse plant developmental processes. however, the mechanisms and functions of grps in salinity stress responses remain largely unknown. in this study, rapid amplification of cdna end (race) pcr methods was adopted to isolate zjgrp from zosyia japonica, a salt-t ...201727796490
in vivo evaluation of the "tinypump" as a pediatric left ventricular assist device.pediatric patients with end-stage heart failure require mechanical circulatory support (mcs) just as adults do. in order to meet the special requirements for neonates' and infants' mcs, pediatric circulatory support devices must be compact with low priming volume, easily controllable with low flow, less traumatic for blood cells and tissues, and biocompatible with minimum anticoagulation. we have designed and developed a miniature rotary centrifugal blood pump, "tinypump," with a priming volume ...201121595723
molecular imaging and contrast agent database (micad)(+)-p-[11c]methylvesamicol ((+)-[11c]pmv) is a radioligand developed for positron emission tomography (pet) imaging of the sigma (σ) receptors. it is a sigma (σ) receptor ligand labeled with 11c, a positron emitter with a physical half-life (t ½) of 20.4 min (1). σ receptors are functional, membrane-bound proteins distributed in the central nervous system (cns) and peripheral organs (2). the cns σ receptors are unique binding sites related to higher brain functions (3). they are distinct from op ...200420641790
ultrasonographic assessment of fetal growth in miniature "shiba" goats (capra hircus).the aim of the present study was to monitor fetal growth in relation to gestational stage to generate formulae which could be used to estimate fetal age in goats. eight miniature shiba goats (capra hircus) were examined weekly by transrectal and transabdominal ultrasound scanning during the gestation period between day 21 and 126 days of gestation. for accurate judgment, all fetometric parameters were measured at least three times per one examination for each animal. quantification of the growth ...201526427952
effects of exposure to male goat hair extracts on luteinizing hormone secretion and neuronal activation in seasonally anestrous ewes.in sheep and goats, exposure of seasonally anestrous females to males or their fleece/hair activates the gonadotropin-releasing hormone (gnrh) pulse generator leading to pulsatile luteinizing hormone (lh) secretion. pheromones emitted by sexually mature males are thought to play a prominent role in this male effect. in the present study, we first aimed to clarify whether the male goat pheromone is effective in ewes. seasonally anestrous st. croix ewes were exposed to hair extracts derived from e ...201424942115
physiological and growth responses of six turfgrass species relative to salinity tolerance.the demand for salinity-tolerant turfgrasses is increasing due to augmented use of effluent or low-quality water (sea water) for turf irrigation and the growing turfgrass industry in coastal areas. experimental plants, grown in plastic pots filled with a mixture of river sand and kosas(r) peat (9 : 1), were irrigated with sea water at different dilutions imparting salinity levels of 0, 8, 16, 24, 32, 40, or 48 ds m⁻¹. salinity tolerance was evaluated on the basis of leaf firing, shoot and root g ...201222666166
distribution of methanogenic potential in fractions of turf grass used as inoculum for the start-up of thermophilic anaerobic digestion.this study aims to investigate thermophilic methanogens in turf used as an inoculum. results showed that methanoculleus sp. regarded as hydrogenotrophic and methanosarcina sp. regarded as acetoclastic methanogens were present in turf tested. however, active acetoclastic methanogens were present in turf soil only. the current study showed that thermophilic methanogens were present in various turf grass species: stenotaphrum secundatum, cynodon dactylon, and zoysia japonica. severe treatments of g ...201222613887
description and sem observations of meloidogyne sasseri n. sp. (nematoda: meloidogynidae), parasitizing beachgrasses.meloidogyne sasseri n. sp. is described and illustrated from american beachgrass (ammophila breviliffulata) originally collected from henlopen state park and fenwick island near the maryland state line in delaware, united states (6). its relationship to m. graminis, m. spartinae, and m. californiensis is discussed. primary distinctive characters of the female perineal pattern were a high to rounded arch with shoulders, widely spaced lateral lines interrupting transverse striations, a sunken vulv ...199319279820
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