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the complete mitochondrial genome of the nematophagous fungus lecanicillium saksenae.lecanicillium saksenae cgmcc5329 is a useful biological control agent against plant-parasitic nematodes. the complete mitogenome sequence of l. saksenae is reported for the first time. the mitochondrial genome is 25 919 bp long with 14 typical protein-coding genes, an intronic orf coding for a putative ribosomal protein (rps3), 2 ribosomal rna genes and a set of 26 transfer rna genes. the phylogeny based on 12 protein-coding genes (except the loss of other two genes in acremonium implicatum) sug ...201726709967
whole genome annotation and comparative genomic analyses of bio-control fungus purpureocillium lilacinum.the fungus purpureocillium lilacinum is widely known as a biological control agent against plant parasitic nematodes. this research article consists of genomic annotation of the first draft of whole genome sequence of p. lilacinum. the study aims to decipher the putative genetic components of the fungus involved in nematode pathogenesis by performing comparative genomic analysis with nine closely related fungal species in hypocreales.201526607873
[effects of pesticides and plant bio-stimulants on the germination of chlamydospores and in vitro development of the nematophagous fungus pochonia chlamydosporia].the effects of pesticides and plant bio-stimulants used in protected vegetable production systems on the fungus pochonia chlamydosporia are unknown.201526422324
fungi predatory activity on embryonated toxocara canis eggs inoculated in domestic chickens (gallus gallus domesticus) and destruction of second stage larvae.the objective of this study was to evaluate the infectivity of toxocara canis eggs after interacting with isolated nematophagous fungi of the species duddingtonia flagrans (ac001) and pochonia chlamydosporia (vc4), and test the predatory activity of the isolated ac001 on t. canis second stage larvae after 7 days of interaction. in assay a, 5000 embryonated t. canis eggs previously in contact with the ac001 and vc4 isolated for 10 days were inoculated into domestic chickens (gallus gallus domesti ...201526032943
endophytic colonization of barley (hordeum vulgare) roots by the nematophagous fungus pochonia chlamydosporia reveals plant growth promotion and a general defense and stress transcriptomic response.plant crop yields are negatively conditioned by a large set of biotic and abiotic factors. an alternative to mitigate these adverse effects is the use of fungal biological control agents and endophytes. the egg-parasitic fungus pochonia chlamydosporia has been traditionally studied because of its potential as a biological control agent of plant-parasitic nematodes. this fungus can also act as an endophyte in monocot and dicot plants, and has been shown to promote plant growth in different agrono ...201525982739
development of a high-efficiency gene knockout system for pochonia chlamydosporia.the nematophagous fungus pochonia chlamydosporia, which belongs to the family clavicipitaceae (ascomycota: pezizomycotina: sordariomycetes: hypocreales), is a promising biological control agent for root-knot and cyst nematodes. its biocontrol effect has been confirmed by pot and field trials. the genome sequence of the fungus was completed recently; therefore, genome-wide functional analyses will identify its infection-associated genes. gene knockout techniques are useful molecular tools to stud ...201525458554
sequencing and functional analysis of the genome of a nematode egg-parasitic fungus, pochonia chlamydosporia.pochonia chlamydosporia is a worldwide-distributed soil fungus with a great capacity to infect and destroy the eggs and kill females of plant-parasitic nematodes. additionally, it has the ability to colonize endophytically roots of economically-important crop plants, thereby promoting their growth and eliciting plant defenses. this multitrophic behavior makes p. chlamydosporia a potentially useful tool for sustainable agriculture approaches. we sequenced and assembled ∼41 mb of p. chlamydosporia ...201424530791
predatory activity of chlamydospores of the fungus pochonia chlamydosporia on toxocara canis eggs under laboratory conditions.the objective of this study was to use chlamydospores of the fungus pochonia chlamydosporia (isolates vc1 and vc4) against toxocara canis eggs in a 15-day in vitro assay. one thousand t. canis eggs were placed in petri dishes containing 2% water agar medium with different concentrations of chlamydospores (1,000, 10,000 or 100,000) of each fungal isolate of p. chlamydosporia (treated groups) and 1,000 eggs in petri dishes without fungus (control group). egg counts were performed to determine the ...201324252967
pochonia chlamydosporia: advances and challenges to improve its performance as a biological control agent of sedentary endo-parasitic nematodes.the nematophagous fungus pochonia chlamydosporia var. chlamydosporia is one of the most studied biological control agents against plant (semi-) endo-parasitic nematodes of the genera globodera, heterodera, meloidogyne, nacobbus and, more recently, rotylenchulus. in this paper we present highlights from more than three decades of worldwide research on this biological control agent. we cover different aspects and key components of the complex plant-fungus-nematode tri-trophic interaction, an inter ...201323589653
the pochonia chlamydosporia serine protease gene vcp1 is subject to regulation by carbon, nitrogen and ph: implications for nematode biocontrol.the alkaline serine protease vcp1 of the fungus pochonia chlamydosporia belongs to a family of subtilisin-like enzymes that are involved in infection of nematode and insect hosts. it is involved early in the infection process, removing the outer proteinaceous vitelline membrane of nematode eggs. little is known about the regulation of this gene, even though an understanding of how nutrients and other factors affect its expression is critical for ensuring its efficacy as a biocontrol agent. this ...201222558192
antagonistic activity of the fungus pochonia chlamydosporia on mature and immature toxocara canis eggs.in vitro tests were performed to evaluate the ability of 6 isolates of the nematophagous fungus pochonia chlamydosporia to infect immature and mature toxocara canis eggs on cellulose dialysis membrane. there was a direct relationship between the number of eggs colonized and the increase in the days of interaction, as well as between the number of eggs colonized and the increase in the concentration of chlamydospores (p<0.05). immature eggs were more susceptible to infection than mature eggs. the ...201222444833
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