| biosynthesis and characterization of copolymer poly(3hb-co-3hv) from saponified jatropha curcas oil by pseudomonas oleovorans. | polyhydroxyalkanoates (phas) are naturally occurring biodegradable polymers with promising application in the formulation of plastic materials. phas are produced by numerous bacteria as energy/carbon storage materials from various substrates, including sugars and plant oils. since these substrates compete as food sources, their use as raw material for industrial-scale production of pha is limited. therefore, efforts have been focused on seeking alternative sources for bacterial production of pha ... | 2010 | 20467780 |
| differential antioxidative enzyme responses of jatropha curcas l. to chromium stress. | chromium (cr) tolerant and accumulation capability of jatropha curcas l. was tested in cr spiked soil amended with biosludge and biofertilizer. plants were cultivated in soils containing 0, 25, 50, 100 and 250 mg kg(-1) of cr for one year with and without amendment. plant tissue analysis showed that combined application of biosludge and biofertilizer could significantly reduce cr uptake and boost the plant biomass, whereas biofertilizer alone did not affect the uptake and plant growth. antioxida ... | 2010 | 20478653 |
| in vitro antiplasmodial activity and toxicity assessment of some plants from nigerian ethnomedicine. | context: the emergence and spread of plasmodium falciparum-resistant parasites to nearly all available antimalarial drugs pose a threat to malaria control and necessitates the need to continue the search for new effective and affordable drugs. ethnomedicine has been shown to be a potential source of antimalarial compounds or source of template for the synthesis of novel antimalarial molecules. objective: the antiplasmodial activity and toxicity assessment of 30 plant extracts from eight medicina ... | 2011 | 20738218 |
| nocardia endophytica sp. nov., a novel endophytic actinomycete isolated from oil-seed plant jatropha curcas l. | a novel actinomycete, designated strain klbmp 1256t, was isolated from the surface-sterilized stem of oil-seed plant jatropha curcas l. collected from sichuan province, south-west china, and was characterized in order to determine its taxonomic position. phylogenetic analyses based on 16s rrna gene sequences indicated that the isolate was closely related to members of the genus nocardia in the family nocardiaceae, being most closely related to nocardia callitridis cap 290t (98.4%) and nocardia n ... | 2010 | 20817835 |
| antibacterial activities of jatropha curcas (linn) on coliforms isolated from surface waters in akure, nigeria. | this study investigated the antibacterial activities of hot water, ethanol and acetone extracts of jatropha curcas (linn) leaves on coliforms isolated from surface waters using growth inhibition indices based on agar plate technique. the percentage recovery of the extracts was 19.17%, 18.10% and 18.80% for hot water, ethanol and acetone respectively. phytochemical screening of the extracts was also determined. qualitative phytochemical screening showed that the plant extracts contained steroids, ... | 2014 | 24711746 |
| pseudonocardia sichuanensis sp. nov., a novel endophytic actinomycete isolated from the root of jatropha curcas l. | a novel isolate, designated strain klbmp 1115(t) was isolated from the surface-sterilized root of oil-seed plant jatropha curcas l. collected from sichuan province, south-west china. characterization of the isolate was based on a polyphasic approach. 16s rrna gene sequence analysis indicated that strain klbmp 1115(t) belongs to the phylogenetic cluster of the genus pseudonocardia and was most closely related to pseudonocardia adelaidensis eum 221(t) (98.9%) and pseudonocardia zijingensis dsm 447 ... | 2011 | 20827574 |
| molecular cloning and characterization of a group ii wrky transcription factor from jatropha curcas, an important biofuel crop. | the wrky family of transcription factors (tfs) play an intricate role in regulating the stress signaling pathways by autoregulation or may be by cross regulation through interaction with other proteins. although wrky tfs are considered to be plant specific, however, their presence has been reported from unicellular algae, slime mould, and gymnosperms. we have isolated the jcwrky cdna from an important biofuel crop jatropha curcas growing in the wastelands of india. the jcwrky gene has an orf of ... | 2014 | 24720696 |
| amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells. | acanthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. jatropha curcas, jatropha gossypifolia and euphorbia milii plant extracts synthesized stable silver nanoparticles (agnps) that were relatively stable. amoebicidal activity of j. gossypifolia, j. curcas and e. milii leaf extracts showed little effect on viability of acanthamoeba castellanii trophozoites. plant-synthesized agnps showed higher amoebicidal activity. agnps synthesized by j. ... | 2013 | 23746354 |
| a melting pot of old world begomoviruses and their satellites infecting a collection of gossypium species in pakistan. | clcud in southern asia is caused by a complex of multiple begomoviruses (whitefly transmitted, single-stranded [ss]dna viruses) in association with a specific ssdna satellite; cotton leaf curl multan betasatellite (clcumub). a further single ssdna molecule, for which the collective name alphasatellites has been proposed, is also frequently associated with begomovirus-betasatellite complexes. multan is in the center of the cotton growing area of pakistan and has seen some of the worst problems ca ... | 2012 | 22899988 |
| effect of various levels of dietary jatropha curcas seed meal on rabbits infested by the adult ticks of hyalomma marginatum marginatum i. animal performance, anti-tick feeding and haemogram. | the goal of this study was to investigate the use of jatropha curcas seed meal (jcsm) in different levels as acaricide in diet of rabbits experimentally infested by hyalomma marginatum marginatum then determining animal performance, anti-tick feeding and its effects on haemogram of rabbits. thirty healthy mixed-breed rabbits were randomly divided into five equal groups. the first group was kept as a control fed soya bean meal (20%) as a source of protein. groups from the second to the fifth fed ... | 2010 | 20882342 |
| synthesis of water soluble quantum dots for monitoring carrier-dna nanoparticles in plant cells. | fluorescent quantum dots (qds) have shown great promise for use as biolabels in cell and animal biology and more recently in plant sciences. an important use of qds is for monitoring the dynamics, intracellular trafficking, and fate of carrier-dna nanocomplexes in cell transfection and potentially in plant transformation. in this study, a low cost aqueous procedure has been developed to efficiently prepare biocompatible qds for monitoring nanoparticle-mediated gene transfer in conjunction with m ... | 2011 | 21449370 |
| structural and functional analyses of a saturated acyl acp thioesterase, type b from immature seed tissue of jatropha curcas. | the distinguishing structural and functional domains of plant acyl-acyl carrier protein (acp) thioesterases and their complex interaction with the acp-linked fatty acid substrate complex have remained elusive. e. coli based heterologous expression and characterisation of many plant thioesterases reported so far have not been extended and linked to in silico modelling studies to explain the diversity in plant thioesterase substrate specificities. in this study, a thioesterase cdna isolated from i ... | 2011 | 21489096 |
| a comparison of polyphenolic content, antioxidant activity and insecticidal properties of jatropha species and wild ricinus communis l. found in mauritius. | to evaluate the phytochemical, antimicrobial and insecticidal properties of different jatropha species and ricinus communis (castor) (r. communis) growing in mauritius. | 2014 | 25312155 |
| co-composting of physic nut (jatropha curcas) deoiled cake with rice straw and different animal dung. | to address the dispensing of this growing volume, a study on utilization of jatropha (jatropha curcas) deoiled cake through compost production was carried out. the deoiled cake was composted with rice straw, four different animal dung (cow dung, buffalo dung, horse dung and goat dung) and hen droppings in different proportions followed by assessment, and comparison of biochemical characteristics among finished composts. nutrient content in finished compost was within the desired level whereas me ... | 2011 | 21489784 |
| an escherichia coli cell membrane chromatography-offline lc-tof-ms method for screening and identifying antimicrobial peptides from jatropha curcas meal protein isolate hydrolysates. | a novel, simple, and rapid method, named cell membrane affinity extraction (cmae)-offline liquid chromatography time-of-flight mass spectrometry (lc-tof-ms) was developed for screening and identifying antimicrobial peptides from jatropha curcas meal protein isolate hydrolysates (jcmpih) obtained by proteolytic enzyme (pepsin, trypsin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease) hydrolysis. a cationic antimicrobial peptide (cailthkr, jcpep8) was successfully isolated and ... | 2012 | 22522648 |
| production of marker-free transgenic jatropha curcas expressing hybrid bacillus thuringiensis δ-endotoxin cry1ab/1ac for resistance to larvae of tortrix moth (archips micaceanus). | the potential biofuel plant jatropha curcas l. is affected by larvae of archips micaceanus (walker), a moth of the family tortricidae. the hybrid bacillus thuringiensis (bt) δ-endotoxin protein cry1ab/1ac confers resistance to lepidopteran insects in transgenic rice. | 2014 | 24808924 |
| plausible exploitation of jatropha de-oiled seed cake for lipase and phytase production and simultaneous detoxification by candida parapsilosis isolated from poultry garbage. | jatropha de-oiled seed cake was explored to utilize as a basic nutrient source for candida parapsilosis, isolated from poultry garbage and selected based on the production of lipase and phytase enzymes under submerged fermentation. at optimized parameters under solid-state fermentation, lipase and phytase activities were recorded as 1056.66±2.92 and 833±2.5u/g of substrate (u/g), respectively. besides enzyme production, complete elimination of phorbol esters and significant phytate reduction fro ... | 2017 | 27894040 |
| amycolatopsis endophytica sp. nov., a novel endophytic actinomycete isolated from oil-seed plant jatropha curcas l. | a novel actinomycete, designated klbmp 1221(t), was isolated from the surface-sterilized seeds of an oil-seed plant jatropha curcas l. collected from sichuan province, south-west china and was characterized taxonomically by using a polyphasic approach. phylogenetic analyses based on 16s rrna gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus amycolatopsis. the 16s rrna gene sequence similarity indicated that strain klbmp 1221(t) was most close ... | 2011 | 21594685 |
| over-expression of jcdreb, a putative ap2/erebp domain-containing transcription factor gene in woody biodiesel plant jatropha curcas, enhances salt and freezing tolerance in transgenic arabidopsis thaliana. | jatropha curcas l. is an all-purpose biodiesel plant and is widely distributed in tropical and subtropical climates. it can grow well on poor quality soil which is not qualified for crop cultivation. this is very important for relieving land, food and energy crises. however, tropical and subtropical distribution limits the production of j. curcas seed. so it is valuable to know the molecular mechanism of j. curcas response to adverse abiotic environmental factors, especially freezing stress, in ... | 2011 | 21958703 |
| a putative flowering-time-related dof transcription factor gene, jcdof3, is controlled by the circadian clock in jatropha curcas. | plant-specific dna-binding transcription factors with one finger (dof) perform important roles in several biological processes. a yeast one-hybrid cdna library of jatropha curcas was used to identify dof-type transcription factors. jcdof3, isolated from the library as a full-length cdna, encoded a protein of 518 amino acids and contained a highly conserved dof domain. yeast one-hybrid systems and subcellular localization assays confirmed that jcdof3 was a typical transcription factor. in contras ... | 2011 | 21958709 |
| Purification, crystallization and preliminary X-ray characterization of a haemagglutinin from the seeds of Jatropha curcas. | The plant Jatropha curcas (Euphorbiaceae) is an important source of biofuel from the inedible oil present in its toxic seeds. The toxicity arises from the presence of curcin, a ribosome-inactivating protein showing haemagglutination activity. In this communication, the purification, crystallization and preliminary X-ray characterization are reported of a small protein isolated from J. curcas seeds with a molecular mass of ~10 kDa that agglutinates rabbit erythrocytes. The protein was crystallize ... | 2011 | 22139159 |
| Efficient free fatty acid production in Escherichia coli using plant acyl-ACP thioesterases. | Microbial biosynthesis of fatty acid-like chemicals from renewable carbon sources has attracted significant attention in recent years. Free fatty acids can be used as precursors for the production of fuels or chemicals. Free fatty acids can be produced by introducing an acyl-acyl carrier protein thioesterase gene into Escherichia coli. The presence of the acyl-ACP thioesterase will break the fatty acid elongation cycle and release free fatty acid. Depending on their sequence similarity and subst ... | 2011 | 22001432 |
| stimulation of the growth of jatropha curcas by the plant growth promoting bacterium enterobacter cancerogenus msa2. | a novel enterobacter cancerogenus msa2 is a plant growth promoting gamma-proteobacterium that was isolated from the rhizosphere of jatropha cucas a potentially important biofuel feed stock plant. based on phenotypic, physiological, biochemical and phylogenetic studies, strain msa2 could be classified as a member of e. cancerogenus. however, comparisons of characteristics with other known species of the genus enterobacter suggested that strain msa2 could be a novel pgpb strain. in vitro studies w ... | 2011 | 22805809 |
| impact of mine waste dumps on growth and biomass of economically important crops. | the present study aimed to investigate the effect of magnesite and bauxite waste dumps on growth and biochemical parameters of some edible and economically important plants such as vigna radiata, v. mungo, v. unguiculata, eleusine coracana, cajanus cajan, pennisetum glaucum, macrotyloma uniflorum, oryza sativa, sorghum bicolour, sesamum indicum, ricinus communis, brassica juncea, gossypium hirsutum and jatropha curcas. the growth rate of all the crops was observed in the range of 75 to 100% in m ... | 2012 | 23741803 |
| antibacterial potential of jatropha curcas synthesized silver nanoparticles against food borne pathogens. | the aqueous leaf extract of jatropha curcas was used for the synthesis of silver nanoparticles (jc-agnps) which were further evaluated for its antibacterial potential against food borne pathogens. j. curcas leaf extract could synthesize stable silver nanoparticles (zeta potential: -23.4 mv) with absorption band at 430 nm. fourier transform infrared spectroscopy indicated various biological compounds responsible for capping and stabilizing jc-agnps in suspension, while the presence of silver was ... | 2016 | 27877160 |
| the use of underwater high-voltage discharges to improve the efficiency of jatropha curcas l. biodiesel production. | underwater high-voltage discharges (3.5 kv) resulting in 4.9 kj shock waves (50-60 mpa) were studied at the laboratory scale as a jatropha curcas l. seed disintegration method. grinding and macerating in an excess of methanol (3.5:1) was advantageous because methanol acts both as a liquid carrier for the pressure shock waves and as a solvent that increases the efficiency of oil extraction while remaining usable for esterification. the influence of the number of shock waves and the intensity of m ... | 2012 | 23586954 |
| molecular cloning, expression analysis, and functional characterization of the h(+)-pyrophosphatase from jatropha curcas. | h(+)-pyrophosphatase (h(+)-ppase) is a primary pyrophosphate (ppi)-energized proton pump to generate electrochemical h(+) gradient for atp production and substance translocations across membranes. it plays an important role in stress adaptation that was intensively substantiated by numerous transgenic plants overexpressing h(+)-ppases yet devoid of any correlated studies pointing to the elite energy plant, jatropha curcas. herein, we cloned the full length of j. curcas h(+)-ppase (jcvp1) complem ... | 2016 | 26643082 |
| cloning, overexpression, purification, and characterization of a new iron superoxide dismutase from jatropha curcas. | a novel iron superoxide dismutase (fe-sod) gene from jatropha curcas was cloned and expressed in escherichia coli bl21 (de3). this recombinant enzyme was isolated by a combined procedure involving immobilized metal-ion affinity chromatography and ion-exchange chromatography. the apparent molecular mass of this purified enzyme (designated as jcfe-sod) was 35 kda on sds-page. the full-length complementary dna sequence of jcfe-sod contained a 918-bp open reading frame encoding a 305-amino-acid prec ... | 2012 | 23586910 |
| intra- specific variation in response of jatropha (jatropha curcas l.) to elevated co2 conditions. | twenty genotypes of jatropha collected from diverse eco-geographic regions from the states of chhattisgarh (3), andhra pradesh (12), rajasthan (4) and uttarakhand (1) of india were subjected to elevated co2 conditions. all the genotypes showed significant difference (p < 0.05 and 0.01) in the phenotypic traits in both the environments (elevated and ambient) and genotype x environment interaction. among the physiological traits recorded, maximum photosynthetic rate was observed in ic565048 (48.8 ... | 2012 | 23573047 |
| a new record of longicorn beetle, acanthophorus rugiceps, from india as a root borer on physic nut, jatropha curcas, with a description of life stages, biology, and seasonal dynamics. | longicorn beetle, acanthophorus rugiceps gahan (coleoptera: cerambycidae), is reported for the first time as a confirmed host on physic nut, jatropha curcas l. (malpighiales: euphorbiaceae), from india, causing extensive damage to roots. plants of three years age and above were prone to attack by this pest. in a six year study beginning in 2005, about 11.3 percent of plants in a 16.25 acre physic nut plantation were severely damaged by a. rugiceps. life stages of a. rugiceps, including egg, larv ... | 2012 | 23461741 |
| gene structures, evolution and transcriptional profiling of the wrky gene family in castor bean (ricinus communis l.). | wrky proteins comprise one of the largest transcription factor families in plants and form key regulators of many plant processes. this study presents the characterization of 58 wrky genes from the castor bean (ricinus communis l., euphorbiaceae) genome. compared with the automatic genome annotation, one more wrky-encoding locus was identified and 20 out of the 57 predicted gene models were manually corrected. all rcwrky genes were shown to contain at least one intron in their coding sequences. ... | 2016 | 26849139 |
| biosynthesis of silver nanoparticles using latex from few euphorbian plants and their antimicrobial potential. | the synthesis of well-dispersed and ultrafine metal nanoparticles has great interest due to their distinctive physicochemical properties and biomedical applications. this study is the first report of one-step solvent-free synthesis of agnps using euphorbiaceae plant latex. among evaluated eight latex-producing plants, four (jatropha curcas, jatropha gossypifolia, pedilanthus tithymaloides, and euphorbia milii) showed high potential to produce physicochemically distinct, small-sized and bacterici ... | 2012 | 22592777 |
| leaf-residing methylobacterium species fix nitrogen and promote biomass and seed production in jatropha curcas. | jatropha curcas l. (jatropha) is a potential biodiesel crop that can be cultivated on marginal land because of its strong tolerance to drought and low soil nutrient content. however, seed yield remains low. to enhance the commercial viability and green index of jatropha biofuel, a systemic and coordinated approach must be adopted to improve seed oil and biomass productivity. here, we present our investigations on the jatropha-associated nitrogen-fixing bacteria with an aim to understand and expl ... | 2015 | 26697111 |
| draft genome sequence of methylobacterium sp. strain l2-4, a leaf-associated endophytic n-fixing bacterium isolated from jatropha curcas l. | methylobacterium sp. strain l2-4 is an efficient nitrogen-fixing leaf colonizer of biofuel crop jatropha curcas. this strain is able to greatly improve the growth and seed yield of jatropha curcas and is the second reported genome sequence of plant growth-promoting bacteria isolated from jatropha curcas. | 2014 | 25502683 |
| lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against haemonchus contortus. | bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. extracts of native and exotic seed species from brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. each seed species was subjected to three extractions with three types of solvents. all the seeds showed ovicidal activity, which varied according to the solvents. higher ovicidal activity was fo ... | 2014 | 25054490 |
| molecular characterization of a new begomovirus associated with leaf yellow mosaic disease of jatropha curcas in india. | during a survey in june 2011, severe leaf yellow mosaic disease was observed on about 45 % plants of jatropha curcas growing in the katerniaghat wildlife sanctuary in india. an association of a begomovirus with disease was detected in 15 out of 20 samples by pcr using begomovirus genus-specific primers and total dna isolated from symptomatic leaf samples. for identification of the begomovirus, the complete genome was amplified using a phi-29 dna-polymerase-based rolling-circle amplification kit ... | 2015 | 25716923 |
| dna-a of a highly pathogenic indian cassava mosaic virus isolated from jatropha curcas causes symptoms in nicotiana benthamiana. | jatropha curcas mosaic disease (jcmd) is a newly emerging disease that has been reported in africa and india. here, we report the complete nucleotide sequence of a new indian cassava mosaic virus isolate (icmv-sg) from singapore. infection of icmv-sg showed more severe jcmd in jatropha curcas and nicotiana benthamiana than the other icmv isolates reported previously, though icmv-sg shares high sequence identity with the other icmv isolates. agroinfectious dna-a alone sufficiently induced systemi ... | 2014 | 24445901 |
| effect of culture media and environmental factors on mycelial growth and pycnidial production of lasiodiplodia theobromae in physic nut (jatropha curcas). | physic nut (jatropha curcas) is an important commercial bio-diesel plant species and is being advocated for development of waste and dry land. the collar and root rot caused by lasiodiplodia theobromae is an important soil borne disease which causes considerable yield loss in this crop. in this study, the effects of culture media, temperature, photoperiod, carbon and nitrogen sources and ph on mycelial growth and pycnidial production were evaluated. among the growth media tested, potato dextrose ... | 2013 | 24640243 |
| bioactive compounds and biological activities of jatropha curcas l. kernel meal extract. | defatted jatropha curcas l. (j. curcas) seed kernels contained a high percentage of crude protein (61.8%) and relatively little acid detergent fiber (4.8%) and neutral detergent fiber (9.7%). spectrophotometric analysis of the methanolic extract showed the presence of phenolics, flavonoids and saponins with values of 3.9, 0.4 and 19.0 mg/g dm, respectively. high performance liquid chromatography (hplc) analyses showed the presence of gallic acid and pyrogallol (phenolics), rutin and myricetin (f ... | 2011 | 22016638 |
| complete genome sequence of enterobacter sp. strain r4-368, an endophytic n-fixing gammaproteobacterium isolated from surface-sterilized roots of jatropha curcas l. | enterobacter sp. strain r4-368 is one of the few characterized jatropha endophytic diazotrophic bacteria and was isolated from surface-sterilized roots. this bacterium shows strong growth-promoting effects, being able to increase plant biomass and seed yields. enterobacter sp. r4-368 is the second fully sequenced diazotrophic enterobacter species. the sequence information shall facilitate the elucidation of the molecular mechanisms of plant growth promotion, nitrogen fixation in nonlegume plant ... | 2013 | 23908287 |
| an efficient protocol for agrobacterium-mediated transformation of the biofuel plant jatropha curcas by optimizing kanamycin concentration and duration of delayed selection. | jatropha curcas is considered a potential biodiesel feedstock crop. currently, the value of j. curcas is limited because its seed yield is generally low. transgenic modification is a promising approach to improve the seed yield of j. curcas. although agrobacterium-mediated genetic transformation of j. curcas has been pursued for several years, the transformation efficiency remains unsatisfying. therefore, a highly efficient and simple agrobacterium-mediated genetic transformation method for j. c ... | 2017 | 26640597 |
| cytotoxic activity of crude extracts as well as of pure components from jatropha species, plants used extensively in african traditional medicine. | extracts from jatropha curcas, a plant used in african traditional medicine for various diseases, were tested for cytotoxic activity. the root extracts strongly reduced cell growth of tumor cells in vitro, a result consistent with the knowledge of the application of these plant extracts in traditional medicine, especially to cure/ameliorate cancer. a selection of pure diterpenoids existing in extracts from jatropha species and isolated from j. curcas, for example, curcusone c, curcusone d, multi ... | 2011 | 21754941 |
| analysis of seed phorbol-ester and curcin content together with genetic diversity in multiple provenances of jatropha curcas l. from madagascar and mexico. | jatropha curcas l. has been promoted as an oilseed crop for use to meet the increased world demand for vegetable oil production, and in particular, as a feedstock for biodiesel production. seed meal is a protein-rich by-product of vegetable oil extraction, which can either be used as an organic fertilizer, or converted to animal feed. however, conversion of j.-ácurcas seed meal into animal feed is complicated by the presence of toxins, though plants producing "edible" or "non-toxic" seeds occur ... | 2011 | 21835630 |
| Expression and characterization of recombinant Rhizopus oryzae lipase for enzymatic biodiesel production. | The Rhizopus oryzae lipase containing prosequence was expressed in Pichia pastoris. Recombinant lipase subunit showed a molecular mass of 32 kDa. The maximum activity of recombinant lipase obtained from Mut(s) recombinant was 90 IU/ml. The enzyme was stable in broad ranges of temperatures and pH, with the optimal temperature at 35 °C and pH 7.0. The crude recombinant R. oryzae lipase can be directly used for the transesterification of plant oils at high-water content of 60-100% (w/w) based on oi ... | 2011 | 21852124 |
| kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant jatropha curcas l. containing 1-aminocyclopropane-1-carboxylic acid deaminase. | a novel actinomycete, designated strain klbmp 1111(t), was isolated from the root of the oil-seed plant jatropha curcas l. collected from sichuan province, south-west china. strain klbmp 1111(t) formed a distinct branch in the 16s rrna gene phylogenetic tree together with the type strains in the genus kibdelosporangium, with the highest similarity to kibdelosporangium aridum subsp. aridum dsm 43828(t) (98.8%), k. aridum subsp. largum dsm 44150(t) (98.1%) and kibdelosporangium philippinense dsm 4 ... | 2011 | 21989686 |
| isolation and functional characterization of jcft, a flowering locus t (ft) homologous gene from the biofuel plant jatropha curcas. | physic nut (jatropha curcas l.) is a potential feedstock for biofuel production because jatropha oil is highly suitable for the production of the biodiesel and bio-jet fuels. however, jatropha exhibits low seed yield as a result of unreliable and poor flowering. flowering locus t (ft) -like genes are important flowering regulators in higher plants. to date, the flowering genes in jatropha have not yet been identified or characterized. | 2014 | 24886195 |
| cloning and characterization of a β-ketoacyl-acyl carrier protein synthase ii from jatropha curcas. | a cdna clone encoding a putative β-ketoacyl-acyl carrier protein (acp) synthase ii (kasii), a key enzyme in fatty acid biosynthesis, was isolated from jatropha curcas l., a woody oil plant. the isolated cdna clone of jckasii contained a 1722-bp open reading frame coding for 573 amino acids with a predicted molecular mass of about 60.98 kda and the conserved cys(324) residues that has been proposed as the active site of kasii proteins. the deduced amino acid sequence of the cdna clone had about 7 ... | 2012 | 22424763 |
| jcti-i: a novel trypsin inhibitor from jatropha curcas seed cake with potential for bacterial infection treatment. | jatropha curcas seed cake is a low-value by-product resulting from biodiesel production. the seed cake is highly toxic, but it has great potential for biotechnology applications as it is a repository of biomolecules that could be important in agriculture, medicine, and industry. to explore this potential, a novel trypsin inhibitor called jcti-i was purified by fractionation of the crude extract with trichloroacetic acid (2.5%, v/v) followed by affinity chromatography (trypsin-sepharose 4b) and m ... | 2014 | 24523715 |
| endophytic fungi isolated from oil-seed crop jatropha curcas produces oil and exhibit antifungal activity. | jatropha curcas l., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. the plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. the plant was explored for its endophytic fungi for use in crop protection. endophytic fungi were isolated from leaf of jatropha curcas, collected from new delhi, india. four isolates were identified as colletotrichum truncatum, and other ... | 2013 | 23409154 |
| bioactivities of jc-scrip, a type 1 ribosome-inactivating protein from jatropha curcas seed coat. | in this study, a type 1 rip, designated as jc-scrip, was first isolated from the seed coat of jatropha curcas linn. it was purified by ammonium sulfate precipitation and chromatography on deae-sephacel™ and cm-cellulose columns. purification fold of jc-scrip increased 113.8 times, and the yield was 1.13% of the total protein in the final step. it was shown to be a monomeric glycoprotein with a molecular mass of 38 938 da, as determined by maldi-tof/ms. it exhibited hemagglutination activity and ... | 2013 | 23773434 |
| molecular identification of a new begomovirus associated with mosaic disease of jatropha curcas l. in nigeria. | we report the complete nucleotide sequence of dna-a of a begomovirus naturally infecting jatropha curcas l. in nigeria. symptoms observed on infected plants were severe mosaic, mottling and blistering of leaves. the virus, which we provisionally name "jatropha mosaic nigeria virus" (jmnv), has a monopartite genome of 2,779 to 2,789 nucleotides. pairwise comparisons of dna-a sequences showed that jmnv had maximum nucleotide sequence identity (72%) with a strain of tomato yellow leaf curl virus. s ... | 2013 | 23074041 |
| bio-detoxification of phorbol esters and other anti-nutrients of jatropha curcas seed cake by fungal cultures using solid-state fermentation. | jatropha seed cake, a byproduct after biodiesel extraction, has several anti-nutrients and toxins. solid-state fermentation was carried out for the detoxification of the jatropha seed cake (jsc) using different fungal cultures. the reduction in the anti-nutritional components such as tannins, phytates, saponins, lectin and protease inhibitor, and phorbol esters on 6th, 9th, and 12th day of fermentation was analyzed. the phorbol ester content in the unfermented jsc was 0.83 mg/g, and the maximum ... | 2014 | 24435764 |
| utilization of biodiesel by-products for mosquito control. | the current paper has elaborated the efficient utilization of non-edible oil seed cakes (neoc), by-products of the bio-diesel extraction process to develop a herbal and novel mosquitocidal composition against the aedes aegypti larvae. the composition consisted of botanical active ingredients, inerts, burning agents and preservatives; where the botanical active ingredients were karanja (pongamia glabra) cake powder and jatropha (jatropha curcas) cake powder, products left after the extraction of ... | 2016 | 26296531 |
| larvicidal activity of a toxin from the seeds of jatropha curcas linn. against aedes aegypti linn. and culex quinquefasciatus say. | the larvicidal effects of the crude protein extract and purified toxin, jc-scrip, from the seed coat of jatropha curcas linn. against the third instar larvae of mosquitoes, aedes aegypti linn. and culex quinquefasciatus say, were investigated. this test compared the effects of the purified toxin with crude protein extracts from seed kernels of j. curcas and ricinus communis. at various concentrations of purified toxin and crude protein extract, the larval mortality of both ae. aegypti and cx. qu ... | 2012 | 22735851 |
| identification of mosquito biting deterrent constituents from the indian folk remedy plant jatropha curcas. | an investigation of the indian folk remedy plant jatropha curcas l., was performed to identify the constituents responsible for the mosquito biting deterrent activity of the oil. j. curcas seed oil is burned in oil lamps in india and parts of africa to repel biting insects, primarily mosquitoes. the seed oil was thoroughly analyzed by 1h nmr, 13c nmr, high-performance liquid chromatography-refractive index, and gas chromatography-flame ionization detection to identify the constituents in the oil ... | 2011 | 21845943 |
| tri-trophic insecticidal effects of african plants against cabbage pests. | botanical insecticides are increasingly attracting research attention as they offer novel modes of action that may provide effective control of pests that have already developed resistance to conventional insecticides. they potentially offer cost-effective pest control to smallholder farmers in developing countries if highly active extracts can be prepared simply from readily available plants. field cage and open field experiments were conducted to evaluate the insecticidal potential of nine com ... | 2013 | 24205287 |
| measuring population fluctuation of jatropha stem-borer [cophes notaticeps (marshall)] in the brazilian cerrado using a new trap. | this study aimed to monitor the population fluctuation of cophes notaticeps (marshall) (coleoptera: curculionidae) in a jatropha (jatropha curcas l.) plantation in the federal district, brazil, through the use of a new trap, combined with different attractive lures and trap colors. the study was conducted at embrapa cerrados (planaltina/df) in a field trial with 720 plants. the new trap, named cpac16, was made with pvc pipes (100 mm in diameter) of about 40 cm in length, with a window (8 × 10 cm ... | 2017 | 28185606 |
| bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. | livestock and aquaculture production is under political and social pressure, especially in the european union (eu), to decrease pollution and environmental damage arising due to animal agriculture. the eu has banned the use of antibiotics and other chemicals, which have been shown to be effective in promoting growth and reducing environment pollutants because of the risk caused to humans by chemical residues in food and by antibiotic resistance being passed on to human pathogens. as a result of ... | 2007 | 22444893 |
| conversion of crude jatropha curcas seed oil into biodiesel using liquid recombinant candida rugosa lipase isozymes. | the versatile candida rugosa lipase (crl) has been widely used in biotechnological applications. however, there have not been feasibility reports on the transesterification of non-edible oils to produce biodiesel using the commercial crl preparations, mixtures of isozymes. in the present study, four liquid recombinant crl isozymes (crl1-crl4) were investigated to convert various non-edible oils into biodiesel. the results showed that recombinant crl2 and crl4 exhibited superior catalytic efficie ... | 2015 | 26011691 |
| survey of the rubber tree genome reveals a high number of cysteine protease-encoding genes homologous to arabidopsis sag12. | arabidopsis thaliana sag12, a senescence-specific gene encoding a cysteine protease, is widely used as a molecular marker for the study of leaf senescence. to date, its potential orthologues have been isolated from several plant species such as brassica napus and nicotiana tabacum. however, little information is available in rubber tree (hevea brasiliensis), a rubber-producing plant of the euphorbiaceae family. this study presents the identification of sag12-like genes from the rubber tree genom ... | 2017 | 28166280 |
| a novel aldo-keto reductase from jatropha curcas l. (jcakr) plays a crucial role in the detoxification of methylglyoxal, a potent electrophile. | abiotic stress leads to the generation of reactive oxygen species (ros) which further results in the production of reactive carbonyls (rcs) including methylglyoxal (mg). mg, an α, β-dicarbonyl aldehyde, is highly toxic to plants and the mechanism behind its detoxification is not well understood. aldo-keto reductases (akrs) play a role in detoxification of reactive aldehydes and ketones. in the present study, we cloned and characterised a putative akr from jatropha curcas (jcakr). phylogeneticall ... | 2016 | 26995646 |
| an acidified thermostabilizing mini-peptide derived from the carboxyl extension of the larger isoform of the plant rubisco activase. | thermostable fusion peptide partners are valuable in engineering thermostability in proteins. we evaluated the arabidopsis counterpart (atrace) and an acidified derivative (mrace) of the conserved carboxyl extension (race) of plant rubisco activase (rca) for their thermostabilizing properties in escherichia coli and saccharomyces cerevisiae using a protein fusion strategy. we used atrace and mrace as fusion tails for the thermolabile protein rca2 from arabidopsis thaliana and nicotiana tabacum. ... | 2015 | 26321073 |
| molecular cloning and characterisation of metallothionein type 2a gene from jatropha curcas l., a promising biofuel plant. | in the present study, we have cloned a gene encoding jcmt2a protein from jatropha curcas l., a promising biofuel tree species. full length sequence of jcmt2a gene was isolated using race pcr. heterologous expression of jcmt2a in escherichia coli and its purification has shown distinct bands corresponding to the gst and gst-fused jcmt2a protein. significant tolerance was observed in e. coli cells expressing recombinant gst-jcmt2a for zinc, copper and cadmium metals compared to cells expressing gs ... | 2014 | 24190491 |
| cloning, expression, and purification of a cu/zn superoxide dismutase from jatropha curcas. | we report cdna cloning, expression, purification, and characterization of a novel cu/ zn superoxide dismutase (sod) from jatropha curcas leaves. the full-length cdna of this sod contained a 496-bp open-reading frame (orf) encoding 162 amino acid residues. the recombinant plasmid containing the sod coding sequence was introduced into escherichia coli, and the sod was expressed as a fusion protein. the recombinant sod was purified from a high-density fed-batch culture using a combination of immobi ... | 2013 | 23659174 |
| pathogenesis-related gene, jcpr-10a from jatropha curcas exhibit rnase and antifungal activity. | the pathogenesis-related proteins have a broad spectrum of roles, ranging from seed germination, development to resistance. the pr-10 is a multigene family differing from other pr proteins in being intracellular, small and acidic with similar 3d structures. we have isolated jcpr-10a cdna with an orf of 483 bp from j. curcas, an important biofuel crop grown in the wastelands of india. jcpr-10a gets clustered with dicots in phylogenetic tree. the genomic organisation analysis of jcpr-10a revealed ... | 2013 | 22763562 |
| jccbf2 gene from jatropha curcas improves freezing tolerance of arabidopsis thaliana during the early stage of stress. | high chilling-susceptibility is becoming the bottleneck for cultivation and commercialization of jatropha curcas l. for insights to chilling resistance ability of this plant species, a cold response transcription factor, jccbf2, was cloned and studied. it codes a 26 kda protein, which contains all conserved motifs unique to the c-repeat binding factor (cbf) family and has high similarity to cbfs of ricinus communis and populus. its transcripts express specifically in leaves of jatropha at cold t ... | 2015 | 25433432 |
| molecular analysis of arf1 expression profiles during development of physic nut (jatropha curcas l.). | a cdna clone designated arf1 was isolated from a physic nut (jatropha curcas l.) endosperm cdna library which encodes a small gtp-binding protein and has significant homology to adp-ribosylation factors (arf) in plants, animals and microbes. the cdna contains an open reading frame that encodes a polypeptide of 181 amino acids with a calculated molecular mass of 20.7 kda. the deduced amino acid sequence showed high homology to known arfs from other organisms. the products of the arf1 obtained by ... | 2011 | 20853149 |
| evaluation of jatropha curcas linn. leaf extracts for its cytotoxicity and potential to inhibit hemagglutinin protein of influenza virus. | influenza is a serious respiratory illness which can be debilitating and cause complications that lead to hospitalization and death. although influenza vaccine can prevent influenza virus infection, the only therapeutic options to treat influenza virus infection are antiviral agents. given temporal and geographic changes and the shifts in antiviral drug resistance among influenza viruses, it is time to consider natural antiviral agents against influenza virus. jatropha curcas is known for variou ... | 2013 | 24426279 |
| lipase-catalyzed biodiesel production and quality with jatropha curcas oil: exploring its potential for central america. | extensive native jatropha curcas l. (jatropha) crop areas have been planted in central america marginal lands since 2008 as a non-edible prospective feedstock alternative to high-value, edible palm oil. jatropha biodiesel is currently exclusively produced in the region at commercial scale utilizing alkaline catalysts. recently, a free, soluble thermomyces lanuginosus (tl) 1,3 specific lipase has shown promise as biocatalyst, reportedly yielding up to 96 % astm d6751 compliant biodiesel after 24 ... | 2015 | 26213567 |
| a first generation microsatellite- and snp-based linkage map of jatropha. | jatropha curcas is a potential plant species for biodiesel production. however, its seed yield is too low for profitable production of biodiesel. to improve the productivity, genetic improvement through breeding is essential. a linkage map is an important component in molecular breeding. we established a first-generation linkage map using a mapping panel containing two backcross populations with 93 progeny. we mapped 506 markers (216 microsatellites and 290 snps from ests) onto 11 linkage groups ... | 2011 | 21901124 |
| sequence analysis of the genome of an oil-bearing tree, jatropha curcas l. | the whole genome of jatropha curcas was sequenced, using a combination of the conventional sanger method and new-generation multiplex sequencing methods. total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. they accounted for ~95% of the gene-containing regions with the average g + c content was 34.3%. a total of 40 929 complete and partial structures of protein encoding genes have been deduced. comparison with genes of o ... | 2011 | 21149391 |
| lipase-mediated conversion of vegetable oils into biodiesel using ethyl acetate as acyl acceptor. | ethyl acetate was explored as an acyl acceptor for immobilized lipase-catalyzed preparation of biodiesel from the crude oils of jatropha curcas (jatropha), pongamia pinnata (karanj) and helianthus annuus (sunflower). the optimum reaction conditions for interesterification of the oils with ethyl acetate were 10% of novozym-435 (immobilized candida antarctica lipase b) based on oil weight, ethyl acetate to oil molar ratio of 11:1 and the reaction period of 12h at 50 degrees c. the maximum yield of ... | 2007 | 16822671 |
| ricinus communis and jatropha curcas (euphorbiaceae) seed oil toxicity against atta sexdens rubropilosa (hymenoptera: formicidae). | leaf-cutting ants are the main herbivores in the new world tropics. although the toxicity of seed oils against these ants has been poorly investigated, previous results revealed that seed oils exert considerable toxic activity against these insects. this paper analyzes the toxic action and deterrent properties of castor oil, ricinus communis l., and physic nut oil, jatropha curcas l., against workers of the leaf-cutting ant atta sexdens rubropilosa reared in laboratory. toxic effect was analyzed ... | 2013 | 23786062 |
| lipase-mediated transformation of vegetable oils into biodiesel using propan-2-ol as acyl acceptor. | propan-2-ol was used as an acyl acceptor for immobilized lipase-catalyzed preparation of biodiesel. the optimum conditions for transesterification of crude jatropha (jatropha curcas), karanj (pongamia pinnata) and sunflower (helianthus annuus) oils were 10% novozym-435 (immobilized candida antarctica lipase b) based on oil weight, alcohol to oil molar ratio of 4:1 at 50 degrees c for 8 h. the maximum conversions achieved using propan-2-ol were 92.8, 91.7 and 93.4% from crude jatropha, karanj and ... | 2006 | 16642301 |
| study on utilization of detoxified jatropha curcas seed cake subjected to solid state fermentation as a dietary supplement in wistar rats. | the presence of anti-nutrients and toxins like phorbol esters in jatropha curcas seed cake (jsc) limits its application in feeds. this study was done to assess the potential of detoxified jsc as rat feed. | 2016 | 27855597 |
| molecular cloning and expression analysis of a novel bccp subunit gene from aleurites moluccana. | aleurites moluccana l. is grown as a roadside tree in southern china and the oil content of its seed is higher than other oil plants, such as jatropha curcas and camellia oleifera. a. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. in addition, the bark, leaves, and kernels of a. moluccana have various medical and commercial uses. here, a novel gene coding the biotin carboxyl carrier protein subunit (bccp) was cloned from ... | 2015 | 26345927 |
| jatropha (jatropha curcas l.). | the seed oil of jatropha (jatropha curcas l.) as a source of biodiesel fuel is gaining worldwide importance. commercial-scale exploration of jatropha has not succeeded due to low and unstable seed yield in semiarid lands unsuitable for the food production and infestation to diseases. genetic engineering is promising to improve various agronomic traits in jatropha and to understand the molecular functions of key jatropha genes for molecular breeding. we describe a protocol routinely followed in o ... | 2015 | 25416246 |
| rnai mediated curcin precursor gene silencing in jatropha (jatropha curcas l.). | curcin, a type i ribosomal inhibiting protein-rip, encoded by curcin precursor gene, is a phytotoxin present in jatropha (jatropha curcas l.). here, we report designing of rnai construct for the curcin precursor gene and further its genetic transformation of jatropha to reduce its transcript expression. curcin precursor gene was first cloned from jatropha strain darl-2 and part of the gene sequence was cloned in sense and antisense orientation separated by an intron sequence in plant expression ... | 2014 | 24574003 |
| treatment of leishmaniasis in the oyapock basin (french guiana): a k.a.p. survey and analysis of the evolution of phytotherapy knowledge amongst wayãpi indians. | cutaneous leishmaniasis is a neglected disease with a high incidence in french guiana, mainly in the middle and upper oyapock basin, where amerindian and some brazilian people live. the main goals of this work were (i) to assess the knowledge about leishmaniasis in the different populations of the middle and upper oyapock basin, (ii) to study the therapeutic strategies adopted by people affected by leishmaniasis and (iii) to document the use of phytotherapeutic remedies for leishmaniasis. knowle ... | 2011 | 21816216 |
| continuous, high-resolution biospeckle imaging reveals a discrete zone of activity at the root apex that responds to contact with obstacles. | shining a laser onto biological material produces light speckles termed biospeckles. patterns of biospeckle activity reflect changes in cell biochemistry, developmental processes and responses to the environment. the aim of this work was to develop methods to investigate the biospeckle activity in roots and to characterize the distribution of its intensity and response to thigmostimuli. | 2014 | 24284818 |
| extraction of high-quality dna from ethanol-preserved tropical plant tissues. | proper conservation of plant samples, especially during remote field collection, is essential to assure quality of extracted dna. tropical plant species contain considerable amounts of secondary compounds, such as polysaccharides, phenols, and latex, which affect dna quality during extraction. the suitability of ethanol (96% v/v) as a preservative solution prior to dna extraction was evaluated using leaves of jatropha curcas and other tropical species. | 2014 | 24761774 |
| detoxification and anti-nutrients reduction of jatropha curcas seed cake by bacillus fermentation. | jatropha curcas seed cake is a by-product generated from oil extraction of j. curcas seed. although it contains a high amount of protein, it has phorbol esters and anti-nutritional factors such as phytate, trypsin inhibitor, lectin and saponin. it cannot be applied directly in the food or animal feed industries. this investigation was aimed at detoxifying the toxic and anti-nutritional compounds in j. curcas seed cake by fermentation with bacillus spp. two gras (generally recognized as safe) bac ... | 2013 | 23014183 |
| laboratory and field evaluation of medicinal plant extracts against filarial vector, culex quinquefasciatus say (diptera: culicidae). | the present study explored the effects of jatropha curcas, hyptis suaveolens, abutilon indicum, and leucas aspera tested against third instar larvae of filarial vector, culex quinquefasciatus. the dried plant materials were powdered by an electrical blender. from each sample, 500 g powder was macerated with 1.5 l of hexane, chloroform, ethyl acetate, and methanol 8h, using soxhlet apparatus, and filtered. the extracts were concentrated at reduced temperature on a rotary evaporator and stored at ... | 2012 | 22146997 |
| ectopic expression of jatropha curcas apetala1 (jcap1) caused early flowering in arabidopsis, but not in jatropha. | jatropha curcas is a promising feedstock for biofuel production because jatropha oil is highly suitable for the production of biodiesel and bio-jet fuels. however, jatropha exhibits a low seed yield as a result of unreliable and poor flowering. apetala1 (ap1) is a floral meristem and organ identity gene in higher plants. the flower meristem identity genes of jatropha have not yet been identified or characterized. to better understand the genetic control of flowering in jatropha, an ap1 homolog ( ... | 2016 | 27168978 |
| isolation and characterization of an ubiquitin extension protein gene (jcuep) promoter from jatropha curcas. | the jcuep promoter is active constitutively in the bio-fuel plant jatropha curcas , and is an alternative to the widely used camv35s promoter for driving constitutive overexpression of transgenes in jatropha. well-characterized promoters are required for transgenic breeding of jatropha curcas, a biofuel feedstock with great potential for production of bio-diesel and bio-jet fuel. in this study, an ubiquitin extension protein gene from jatropha, designated jcuep, was identified to be ubiquitously ... | 2015 | 25502690 |
| functional characterization of two microsomal fatty acid desaturases from jatropha curcas l. | linoleic acid (la, c18:2) and α-linolenic acid (ala, c18:3) are polyunsaturated fatty acids (pufas) and major storage compounds in plant seed oils. microsomal ω-6 and ω-3 fatty acid (fa) desaturases catalyze the synthesis of seed oil la and ala, respectively. jatropha curcas l. seed oils contain large proportions of la, but very little ala. in this study, two microsomal desaturase genes, named jcfad2 and jcfad3, were isolated from j. curcas. both deduced amino acid sequences possessed eight hist ... | 2013 | 23796520 |
| development of methodologies for virus detection in soybean and wheat seeds. | seeds that contain large amounts of oil, starch, fibers and phenols are the most difficult tissues for rna extraction. currently, there are some reports of virus detection in seeds using commercial kits for rna extraction. however, individual seeds were used, which may not be always suitable for analyses that deal with large amounts of seeds. sangha [1] described a simple, quick and efficient protocol for rna extraction and downstream applications in a group of seeds of jatropha (jatropha curcas ... | 2016 | 27408831 |
| a protein extract and a cysteine protease inhibitor enriched fraction from jatropha curcas seed cake have in vitro anti-toxoplasma gondii activity. | toxoplasma gondii is a parasite of great medical and veterinary importance that has worldwide distribution and causes toxoplasmosis. there are few treatments available for toxoplasmosis and the search for plant extracts and compounds with anti-toxoplasma activity is of utmost importance for the discovery of new active drugs. the objective of this study was to investigate the action of a protein extract and a protease inhibitor enriched fraction from j. curcas seed cake on developing tachyzoites ... | 2015 | 25816973 |
| eag responses of apis cerana to floral compounds of a biodiesel plant, jatropha curcas (euphorbiaceae). | the eastern honey bee, [apis cerana (f.)], is an important and common pollinator for an important biodiesel tree, [jatropha curcas (l.)]. to understand sensitivity of a. cerana to different floral compounds, we quantified volatile floral compounds of j. curcas, then determined electroantennogram (eag) responses of a. cerana to 11 compounds each at five doses (0.4, 4, 40, 400, and 4,000 microg) of six most active floral compounds. our results demonstrated that floral compounds of j. curcas differ ... | 2013 | 24020278 |
| manipulation of auxin response factor 19 affects seed size in the woody perennial jatropha curcas. | seed size is a major determinant of seed yield but few is known about the genetics controlling of seed size in plants. phytohormones cytokinin and brassinosteroid were known to be involved in the regulation of herbaceous plant seed development. here we identified a homolog of auxin response factor 19 (jcarf19) from a woody plant jatropha curcas and genetically demonstrated its functions in controlling seed size and seed yield. through virus induced gene silencing (vigs), we found that jcarf19 wa ... | 2017 | 28102350 |
| an ortholog of leafy in jatropha curcas regulates flowering time and floral organ development. | jatropha curcas seeds are an excellent biofuel feedstock, but seed yields of jatropha are limited by its poor flowering and fruiting ability. thus, identifying genes controlling flowering is critical for genetic improvement of seed yield. we isolated the jclfy, a jatropha ortholog of arabidopsis thaliana leafy (lfy), and identified jclfy function by overexpressing it in arabidopsis and jatropha. jclfy is expressed in jatropha inflorescence buds, flower buds, and carpels, with highest expression ... | 2016 | 27869146 |
| isolation and characterization of the jatropha curcas apetala1 (jcap1) promoter conferring preferential expression in inflorescence buds. | the 1.5 kb jcap1 promoter from the biofuel plant jatropha curcas is predominantly active in the inflorescence buds of transgenic plants, in which the -1313/-1057 region is essential for maintaining the activity. arabidopsis thaliana apetala1 (ap1) is a mads-domain transcription factor gene that functions primarily in flower development. we isolated a homolog of ap1 from jatropha curcas (designated jcap1), which was shown to exhibit flower-specific expression in jatropha. jcap1 is first expressed ... | 2016 | 27095108 |
| heterologous expression of two jatropha aquaporins imparts drought and salt tolerance and improves seed viability in transgenic arabidopsis thaliana. | drought and high salinity are environmental conditions that cause adverse effects on the growth and productivity of crops. aquaporins are small integral membrane proteins that belong to the family of the major intrinsic proteins (mips), with members in animals, plants and microbes, where they facilitate the transport of water and/or small neutral solutes thereby affecting water balance. in this study we characterized two aquaporin genes namely, plasma membrane intrinsic protein (pip2;7) and tono ... | 2015 | 26067295 |
| a cu/zn superoxide dismutase from jatropha curcas enhances salt tolerance of arabidopsis thaliana. | superoxide dismutases (sods) are involved in protecting plants against diverse biotic and abiotic stresses. in the present study, a novel cu/zn-sod gene (jccu/zn-sod) was cloned from jatropha curcas l. quantitative reverse transcription-polymerase chain reaction analysis revealed that jccu/zn-sod is constitutively expressed in different tissues of j. curcas and induced under nacl treatment. to characterize the function of this gene with respect to salt tolerance, the construct p35s:jccu/zn-sod w ... | 2015 | 25867355 |
| gene silencing of sugar-dependent 1 (jcsdp1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in jatropha curcas. | triacylglycerols (tags) are the most abundant form of storage oil in plants. they consist of three fatty acid chains (usually c16 or c18) covalently linked to glycerol. sdp1 is a specific lipase for the first step of tag catabolism in arabidopsis seeds. arabidopsis mutants deficient in sdp1 accumulate high levels of oils, probably due to blockage in tag degradation. we applied this knowledge from the model plant, arabidopsis thaliana, to engineer increased seed oil content in the biodiesel plant ... | 2014 | 24606605 |
| jclea, a novel lea-like protein from jatropha curcas, confers a high level of tolerance to dehydration and salinity in arabidopsis thaliana. | jatropha curcas l. is a highly drought and salt tolerant plant species that is typically used as a traditional folk medicine and biofuel crop in many countries. understanding the molecular mechanisms that underlie the response to various abiotic environmental stimuli, especially to drought and salt stresses, in j. curcas could be important to crop improvement efforts. in this study, we cloned and characterized the gene for a late embryogenesis abundant (lea) protein from j. curcas that we design ... | 2013 | 24391737 |
| over-expression of jcdgat1 from jatropha curcas increases seed oil levels and alters oil quality in transgenic arabidopsis thaliana. | the increasing consumption of fossil fuels and petroleum products is leading to their rapid depletion and is a matter of concern around the globe. substitutes of fossil fuels are required to sustain the pace of economic development. in this context, oil from the non food crops (biofuel) has shown potential to substitute fossil fuels. jatropha curcas is an excellent shrub spread and naturalized across the globe. its oil contains a high percentage of unsaturated fatty acids (about 78-84% of total ... | 2013 | 24125179 |
| long-term chemiluminescence signal is produced in the course of luminol oxidation catalyzed by enhancer-independent peroxidase purified from jatropha curcas leaves. | isoenzyme c of horseradish peroxidase (hrp-c) is widely used in enzyme immunoassay combined with chemiluminescence (cl) detection. for this application, hrp-c activity measurement is usually based on luminol oxidation in the presence of hydrogen peroxide (h2o2). however, this catalysis reaction was enhancer dependent. in this study, we demonstrated that jatropha curcas peroxidase (jcgp1) showed high efficiency in catalyzing luminol oxidation in the presence of h2o2. compared with hrp-c, the jcgp ... | 2015 | 25511847 |
| genome-wide identification of the jatropha curcas myb family and functional analysis of the abiotic stress responsive gene jcmyb2. | the myb family is one of the most abundant transcription factor families in plants. myb proteins are involved in plant development, abiotic stress tolerance, hormone signal transduction and disease resistance. here we perform genome-wide identification of myb family transcription factors in an energy plant j. curcas, including determining family composition, phylogenetic evolution and functional prediction analysis. in addition, we further elucidate the function of the jcmyb2 gene. | 2016 | 27004683 |
| molecular cloning, expression analysis, and preliminarily functional characterization of the gene encoding protein disulfide isomerase from jatropha curcas. | reactive oxygen species (ros) in plants, arising from various environmental stresses, impair the thiol-contained proteins that are susceptible to irregular oxidative formation of disulfide bonds, which might be alleviated by a relatively specific modifier called protein disulfide isomerase (pdi). from our previous data of the transcriptome and digital gene expression of cold-hardened jatropha curcas, a pdi gene was proposed to be cold-relevant. in this study, its full-length cdna (jcpdi) was clo ... | 2015 | 25825250 |