| development of loop-mediated isothermal amplification (lamp) for detection of babesia gibsoni infection in dogs. | diagnosis of canine babesiosis, caused by babesia gibsoni is difficult, especially in chronically infected dogs. a loop mediated isothermal amplification (lamp) assay was developed and standardized by using four oligonucleotide primers targeting the hypervariable region of 18s rrna gene (genbank acc. no. kc461261). the primers specifically amplified b. gibsoni dna, while no amplification was detected with dna from non-infected dogs as well as from dogs infected with babesia canis vogeli, hepatoz ... | 2015 | 25749021 |
| development and evaluation of serodiagnostic assays with recombinant bgsa1 of babesia gibsoni. | indirect elisa, dot-elisa and double antibody sandwich elisa (das-elisa) using truncated recombinant bgsa1 (rbgsa1) were developed for detecting babesia gibsoni infection in naturally infected dogs. truncated bgsa1 gene of 858 bp, encoding 32 kda protein was cloned in pet-32a(+) expression vector, expressed in escherichia coli and the recombinant protein was purified under native conditions. to evaluate the ability of the truncated rbgsa1as serodiagnostic reagent for b. gibsoni infection, a pane ... | 2014 | 25241636 |
| optimization of a molecular method for the diagnosis of canine babesiosis. | babesiosis is a hemolytic disease caused by protozoans of the genus babesia (apicomplexa). this disease occurs worldwide and is transmitted by ticks to a variety of mammals, including humans. the objective of the present study was to optimize a molecular approach for the detection of a fragment of 18s rdna of babesia canis, babesia vogeli, babesia rossi or babesia gibsoni based on a single semi-nested polymerase chain reaction (pcr), and compare the efficiency of this approach with that of a sim ... | 2014 | 24728371 |
| serosurvey for tick-borne diseases in dogs from the eastern amazon, brazil. | canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in brazilian dogs. few studies have focused attention in surveying tick-borne diseases in the brazilian amazon region. a total of 129 blood samples were collected from dogs living in the brazilian eastern amazon. seventy-two samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from santarém municipality were collected. serum samples were submitted to indirect immunofluorescence a ... | 2013 | 23802236 |
| identification of babesia species infecting dogs using reverse line blot hybridization for six canine piroplasms, and evaluation of co-infection by other vector-borne pathogens. | canine infection by vector-borne hemoparasites is frequent in tropical and sub-tropical areas where exposure to hematophageous ectoparasites is intensive. a reverse line blot (rlb) assay was designed to improve the simultaneous detection of all named canine piroplasm species combined with other vector-borne pathogens of dogs including ehrlichia canis, hepatozoon canis and leishmania infantum common in the mediterranean basin. blood samples of 110 dogs from spain (n=21), portugal (n=14) and israe ... | 2013 | 23017370 |
| first evidence and molecular characterization of babesia vogeli in naturally infected dogs and rhipicephalus sanguineus ticks in southern france. | babesiosis is an emerging tick-borne disease of animals and humans caused by intraerythrocytic protozoa of the genera babesia and theileria. in france canine babesiosis has a high prevalence with babesia canis thought to be the main aetiological agent of the disease. babesia vogeli has already been reported to occur in europe and in other countries around the mediterranean sea. the tick rhipicephalus sanguineus, the main known vector of b. vogeli, occurs in southern france. however, only one cas ... | 2012 | 22342131 |
| critical analysis of vector-borne infections in dogs: babesia vogeli, babesia gibsoni, ehrlichia canis and hepatozoon canis in punjab, india. | there are few published studies on various vector borne diseases of dogs in india and most depict clinical infection in dogs, diagnosed by observation of the haemopathogens in stained blood smears. this study provides the first report regarding molecular confirmation and ancestral relationship analysis of blood smears positive cases of assorted haemopathogens in punjab province of india. on blood smear examination, haemopathogens were observed in 124 out of 778 (15.95%, 95% ci: 13.53- 18.68) blo ... | 2016 | 27787221 |
| molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine. | ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ... | 2016 | 26969489 |
| mortality in virulent canine babesiosis is associated with a consumptive coagulopathy. | the inflammatory response to infection can activate the coagulation system via complex interactions. if uncontrolled, this may lead to a consumptive coagulopathy, a major risk factor for a poor clinical outcome. this prospective observational study was conducted to determine whether consumptive coagulopathy in dogs with babesia rossi infection is related to mortality. seventy-two client-owned dogs diagnosed with canine babesiosis were included. diagnosis was confirmed by polymerase chain reactio ... | 2013 | 23098634 |
| molecular detection of vector-borne pathogens in wild and domestic carnivores and their ticks at the human-wildlife interface. | urbanization of natural areas is considered one of the causes of the current apparent emergence of infectious diseases. carnivores are among the species that adapt well to urban and periurban environments, facilitating cross-species disease transmission with domestic dogs and cats, and potentially with their owners. the prevalence of vector-borne pathogens (vbp) of zoonotic and veterinary interest was studied in sympatric wild and domestic carnivores into barcelona metropolitan area (ne spain). ... | 2016 | 26643497 |
| is the free-ranging jaguar (panthera onca) a reservoir for cytauxzoon felis in brazil? | this study investigated the occurrence of cytauxzoon felis and babesia spp. in free-ranging jaguars (panthera onca), domestic dogs (canis lupus familiaris) and domestic cats (felis catus) from the cerrado, amazon and pantanal biomes of brazil. blood samples were collected from 30 jaguars, 129 dogs and 22 cats for detection of the 18s rrna genes of piroplasmids. all of the jaguars from the pantanal (n=22) and cerrado (n=4) and three of four jaguars from the amazon were positive for c. felis, but ... | 2017 | 28196774 |
| rangelia vitalii, babesia spp. and ehrlichia spp. in dogs in passo fundo, state of rio grande do sul, brazil. | pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents babesia vogeli and ehrlichia canis and conventional pcr for babesia spp. (gene 18s rr ... | 2016 | 27334817 |
| rangelia vitalii, babesia spp. and ehrlichia spp. in dogs in passo fundo, state of rio grande do sul, brazil. | pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents babesia vogeli and ehrlichia canis and conventional pcr for babesia spp. (gene 18s rr ... | 2016 | 27304518 |
| identification of vector-borne pathogens in dogs and cats from southern brazil. | dogs and cats are often infected with vector-borne pathogens and play a crucial role as reservoirs and hosts in their life cycles. the aim of the present study was to investigate the occurrence of vector-borne pathogens among dogs and cats in the northwestern region of rio grande do sul (rs) state, brazil. one hundred and ten blood samples were collected from dogs (n=80) and cats (n=30). laboratory analysis were carried out through stained blood smears, indirect enzyme-linked immunosorbent assay ... | 2016 | 27266811 |
| seroprevalence of tick-borne pathogens and tick infestation in dogs from tapirapé and karajá indigenous communities, brazil. | with the aim of studying some tick-borne diseases, a total of 327 dogs (114 from tapirapé and 213 from karajá indigenous ethnicity, mato grosso, mt, brazil) were sampled. serum samples were submitted to the indirect fluorescence antibody test (ifat) to detect antibodies against babesia vogeli, ehrlichia canis, and rickettsia spp. possible associations of risk factors and the occurrence of seroreativity to tick-borne agents and tick infestations were analyzed through chi-squared tests. among 327 ... | 2015 | 26186512 |
| survey of ehrlichia canis, babesia spp. and hepatozoon spp. in dogs from a semiarid region of brazil. | this study assessed the occurrence of ehrlichia spp., babesia spp. and hepatozoon spp. infections in 100 tick-harboring dogs from a semiarid region of the state of paraíba, northeastern brazil. blood samples and ticks were collected from the animals, and a questionnaire was submitted to dog owners to obtain general data. blood samples were used to perform hemogram, direct blood smear and immunological and molecular hemoparasite detection. the 1,151 ticks collected were identified as rhipicephalu ... | 2015 | 25909253 |
| a serological and molecular survey of babesia vogeli, ehrlichia canis and rickettsia spp. among dogs in the state of maranhão, northeastern brazil. | this study evaluated exposure and infection by tick-borne agents (babesia vogeli, ehrlichia canis and rickettsia spp.) in 172 dogs in rural areas and 150 dogs in urban areas of the municipality of chapadinha, state of maranhão, northeastern brazil, using molecular and serological methods. overall, 16.1% of the sampled dogs (52/322) were seroreactive to b. vogeli, with endpoint titers ranging from 40 to 640. for e. canis, 14.6% of the dogs (47/322) were seroreactive, with endpoint titers from 80 ... | 2015 | 25909250 |
| detection and molecular identification of hepatozoon canis and babesia vogeli from domestic dogs in palestine. | dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. this study aimed to observe tick borne pathogens in dogs from palestine including hepatozoon canis and babesia species. the prevalence of both h. canis and babesia species infections in apparently healthy dogs, from ten districts of the west bank was surveyed. dna was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). a primer set that amplifi ... | 2017 | 27938422 |
| apparent tick paralysis by rhipicephalus sanguineus (acari: ixodidae) in dogs. | certain tick species including ixodes holocyclus can inoculate neurotoxins that induce a rapid, ascending flaccid paralysis in animals. rhipicephalus sanguineus, the most widespread tick of dogs, is recognized as a vector of several pathogens causing diseases in dogs and humans. a single report suggests its role as cause of paralysis in dogs. this study presents the clinical history of 14 young dogs heavily infested by r. sanguineus (intensity of infestation, 63-328) in an endemic area of southe ... | 2012 | 22546547 |
| acute phase proteins response in cats naturally infected with hepatozoon felis and babesia vogeli. | the measurement of acute phase proteins (app) is being increasingly used in human and veterinary medicine in diagnosis, prognosis, treatment monitoring, and in general health screening. however, information about the app response in cats infected with agents of vector-borne diseases is lacking. | 2017 | 28160310 |
| molecular prevalence and genetic characterization of piroplasms in dogs from tunisia. | in this study, the prevalence of piroplasms in dogs was assessed using polymerase chain reaction (pcr) to identify babesia and theileria species in 200 dogs from northern and central tunisia between spring and autumn 2014. the overall molecular prevalence for piroplasms was 14·5% ± 0·05 (29/200); pcr detected 2 species, namely babesia vogeli and theileria annulata with an overall prevalence of 12·5 ± 0·04 and 2% ± 0·02, respectively. no differences in the molecular prevalences of b. vogeli were ... | 2016 | 27417681 |
| molecular analysis of rhipicephalus sanguineus (acari: ixodidae), an incriminated vector tick for babesia vogeli in taiwan. | the genetic identity of rhipicephalus sanguineus tick was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 32 strains of ticks representing six species of rhipicephalus, two species of dermacentor and two outgroup species (haemaphysalis inermis and ixodes ricinus). seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony ... | 2016 | 27815655 |
| a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli. | babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ... | 2017 | 28739301 |
| a pcr survey of vector-borne pathogens in different dog populations from turkey. | in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ... | 2017 | 28682765 |
| a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia. | canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ... | 2017 | 28673460 |
| first molecular detection of piroplasm infection in pet dogs from gansu, china. | babesiosis, the hemolytic disease caused by babesia, which is a tick-transmitted obligate intraerythrocytic protozoan parasite. this disease is responsible for significant mortality and morbidity rates and enormous economic losses to the livestock industry in tropical and subtropical regions worldwide. in this study, blood samples were collected from 141 pet dogs from gansu, china, and analyzed for babesia or theileria spp. infection using specific pcr and sequencing based on 18s rrna gene fragm ... | 2017 | 28638376 |
| molecular detection of vector-borne pathogens in dogs and cats from qatar. | vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries. | 2017 | 28633666 |
| molecular identification of babesia spp. in naturally infected dogs of kerala, south india. | canine babesiosis is a tick borne haemoprotozoan disease caused by large and small intraerythrocytic apicomplexan piroplasms of the genus babesia spp. the clinical manifestations of the disease vary from inapparent subclinical form to hyperacute shock related haemolytic crisis. microscopic examination of blood smears from suspected dogs revealed babesia canis and b. gibsoni in 3.45 and 25.86 % of samples respectively. a seminested pcr based on previously published species specific primers target ... | 2017 | 28615859 |
| first report on babesia vogeli infection in dogs in the philippines. | babesia vogeli is a tick-borne protozoal pathogen that infects erythrocytes. in southeast asia, this pathogen has only been reported in thailand. in this study, nine dogs presented at three different veterinary clinics in cebu city, philippines were found positive for b. vogeli. dna was extracted from blood samples and tested using a pcr for genus babesia and a pcr specific for b. vogeli (both based on the 18s rrna gene). blood smears (triplicate) from each sample were found negative. all positi ... | 2017 | 27713098 |
| first detection and molecular identification of babesia vogeli from rhipicephalus sanguineus (acari: ixodidae) in taiwan. | a total of 578 rhipicephalus sanguineus ticks collected from dogs in taiwan were examined for babesia by species-specific polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia dna was detected in 1.04 % (6/578) of rh. sanguineus ticks. phylogenetic analysis revealed that these babesia spp. were genetically linked to the same clade within the genospecies of babesia vogeli and could be discriminated from other genospecies of babesia. intra-species anal ... | 2016 | 26796569 |
| molecular characterization of babesia vogeli in dogs from belém, northern brazil. | babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the babesia genus (apicomplexa). little is known about this genus; therefore, this study conducted a molecular characterization of babesia spp in naturally infected dogs in northern brazil. blood samples were collected from 172 dogs from metropolitan belém, and screened for babesia spp using semi-nested polymerase chain reactions. subsequently, 18s rdna gene fragments were amplified and sequenced ... | 2015 | 26662431 |
| investigation of the 8-hydroxy-2'-deoxyguanosine, total antioxidant and nitric oxide levels of serum in dogs infected with babesia vogeli. | oxidative stress was defined as corruption of balance between oxidant-antioxidant states in favor of oxidants. in this study, it was aimed to determine oxidative stress in naturally infected dogs with babesia vogeli. the 8-hydroxy-2'-deoxyguanosine (8-ohdg), nitric oxide (no) and total antioxidant capacity (tac) levels in the sera were analyzed by elisa. 8-ohdg levels increased in b. vogeli infected dogs compared to control group (p<0.05). also, no levels increased while toc levels decreased in ... | 2014 | 24882747 |
| babesia vogeli in a quarantined dog. | | 2013 | 23457280 |
| detection and molecular characterization of piroplasms species from naturally infected dogs in southeast brazil. | rangelia vitalii is a protozoon described from dogs in the south and southeast regions of brazil. it is phylogenetically related to babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. the aim of this work was to detect piroplasm species in dogs in the state of rio de janeiro, brazil, by 18s rrna gene-based pcr assay, restriction fragment length polymorphism (rflp) and sequence analyses. of 103 dogs examined, seven (6.8%) were positive for babesia spp. by pcr. th ... | 2013 | 22832754 |
| vector-borne and zoonotic diseases of dogs in north-west new south wales and the northern territory, australia. | vector-borne diseases of dogs in australian aboriginal communities are relatively unexplored. these dogs represent a unique group with variable ecto- and endo-parasitic burdens, nutritional stresses and a general lack of veterinary intervention. we investigated haemoprotozoal and bacterial pathogen prevalences in relation to erythrocyte and platelet numbers in dogs from north-west new south wales (n-w nsw) and the northern territory (nt; central australia). | 2017 | 28810870 |
| an elisa for the early diagnosis of acute canine babesiosis detecting circulating antigen of large babesia spp. | babesia canis is the predominant babesia species in dogs in europe and is responsible for a severe and fatal disease. an increase in global pet tourism and a widening of the geographic distribution of the tick vector has led to the emergence of infections in areas where previously only imported cases have been reported. due to the potential for rapid and serious disease progression, direct parasite detection by stained blood smears and light microscopy or dna-based methods have traditionally bee ... | 2017 | 28807287 |