| identification and characterization of a serine protease inhibitor esserpin from the chinese mitten crab eriocheir sinensis. | serine protease inhibitors (serpins) represent an expanding superfamily of endogenous inhibitors that regulate proteolytic events and involve in a variety of physiological processes. a serine protease inhibitor, namely esserpin, was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) analysis. the full-length cdna of esserpin was of 2367 bp, including an open reading frame (orf) of 1371 bp encoding a polypeptide of 456 amino acids with estimated molecular ... | 2013 | 23567854 |
| characterization and function analysis of an anti-lipopolysaccharide factor (alf) from the chinese shrimp fenneropenaeus chinensis. | anti-lipopolysaccharide factor (alf) is one of the widely-studied antimicrobial peptides (amps) with broad-spectrum antibacterial activity and antiviral property. previous studies show the existence of multiform of alfs in crustacean which are important for immunity of the animals. in the present study, we characterized one isoform of alf from the chinese shrimp fenneropenaeus chinensis (fcalf2). tissue distribution analysis revealed that fcalf2 showed the highest expression level in the lymphoi ... | 2014 | 24879940 |
| a novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (yhv-8) of yellow head virus. | a new genotype of yellow head virus (yhv), designated as yhv-8, was found in farmed shrimp fenneropenaeus chinensis suffering suspectedly from ems/ahpns (early mortality disease/acute hepatopancreatic necrosis disease) in china in 2012. in this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rrt-lamp) assay was developed for better detection of both genotypes of yhv-1 and yhv-8. a set of six specific primers was successfully designed targeting a conserv ... | 2016 | 27221155 |
| comparison of protein expression profiles of the hepatopancreas in fenneropenaeus chinensis challenged with heat-inactivated vibrio anguillarum and white spot syndrome virus. | fenneropenaeus chinensis (chinese shrimp) culture industry, like other penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. to better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. firstly, the soluble hepatopancreas protein samples in adult chinese shrimp among control, heat-inactivated vibrio-challenged and white spot syndrome virus-infected groups were separated ... | 2014 | 24057166 |
| characterization of an immune deficiency homolog (imd) in shrimp (fenneropenaeus chinensis) and crayfish (procambarus clarkii). | the immune deficiency (imd) signal pathway mediates immunity against gram-negative bacteria in drosophila. recent studies show that the imd pathway also involves in antiviral innate immune responses. the functions of the pathway in crustacean immunity are largely unknown. in this paper, two imds (fcimd and pcimd), one of the key elements of the imd pathway, were identified from chinese white shrimp fenneropenaeus chinensis and red swamp crayfish procambarus clarkii. both proteins have a death do ... | 2013 | 23850721 |
| shrimp myd88 responsive to bacteria and white spot syndrome virus. | the myeloid differentiation factor 88 (myd88) is an important adapter protein which links members of the toll-like receptor (tlr) to the downstream components to activate related signaling pathways. in the present study, a myd88 homolog (fcmyd88) was cloned from penaeid shrimp fenneropenaeus chinensis. the orf of fcmyd88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (dd) and a typical tlr and interleukin-1 receptor (il-1r)-related (tir) domain. homo ... | 2013 | 23238389 |
| involvement of fenneropenaeus chinensis cathepsin c in antiviral immunity. | cathepsin c (cath c) is a lysosomal cysteine protease that belongs to the papain superfamily. cath c is capable of activating many chymotrypsin-like serine proteases and is reported to be a central coordinator for the activation of many serine proteinases in immune and inflammatory cells. in this study, cath c cdna was cloned from fenneropenaeus chinensis (fc). the complete cdna of fc-cath c in chinese white shrimp was found to be 1445-base pairs (bp) long. it contained an open reading frame (or ... | 2012 | 22885030 |
| complete genome sequence of an isolate of a novel genotype of yellow head virus from fenneropenaeus chinensis indigenous in china. | genotype 8 of yellow head virus (yhv-8) was identified recently, but the complete genome sequence of this new genotype has not been reported. in this study, the complete genome of yhv-8 isolate 20120706 collected from hebei province of china in 2012 was sequenced. it was found to be 26,769 nucleotides (nt) in length, including a 20,060-nt open reading frame 1 (orf1), a 435-nt orf2, and a 4971-nt orf3. isolate 20120706 shared 79.7-83.9% nucleotide sequence identity with all seven of the complete ... | 2017 | 28044194 |
| haemocyanin content of shrimp (fenneropenaeus chinensis) associated with white spot syndrome virus and vibrio harveyi infection process. | haemocyanin (hc) is frequently reported to vary significantly by physiological status and environmental stress in crustaceans. in this paper, the shrimp fenneropenaeus chinensis was infected with different concentrations of white spot syndrome virus (wssv) and vibrio harveyi. then, the variation of hc and total protein content of the haemolymph (tpch) were investigated using the established double antibody sandwich enzyme linked immunosorbent assay (das-elisa) and coomassie brilliant blue method ... | 2016 | 26616234 |
| virus-derived small rnas in the penaeid shrimp fenneropenaeus chinensis during acute infection of the dna virus wssv. | small interfering rnas (sirnas) and micrornas (mirnas) are two classes of small rnas (srnas) that are critical for virus-host interplay via the rna interference (rnai) pathway. one virus-derived sirna and numerous mirnas has been reported for the double-stranded dna virus white spot syndrome virus (wssv), however, the expression profiles of these different types of srnas have not been assessed. here, by sequencing the srnas and mrnas of wssv-infected chinese shrimp (fenneropenaeus chinensis), we ... | 2016 | 27349643 |
| dietary supplementation of probiotic bacillus pc465 isolated from the gut of fenneropenaeus chinensis improves the health status and resistance of litopenaeus vannamei against white spot syndrome virus. | this study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic bacillus pc465 on the growth, health status, and disease resistance of litopenaeus vannamei. shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) cfu g(-1)]. probiotic supplementation significantly increased the weight gain and survival of l. vannamei (p < 0.05). the effect of ... | 2016 | 27177431 |
| an evolutionarily ancient no synthase (nos) in shrimp. | nitric oxide (no) is a well known essential molecule that is involved in multiple functions such as neuron transduction, cardiac disease, immune responses, etc.; nitric oxide synthase (nos) is a critical enzyme that catalyzes the synthesis of it. a very few crustacean nos molecules were biochemically characterized so far. in the present study, we cloned and characterized a nos cdna from haemocytes of tiger shrimp (penaeus monodon) (pmnos). the full-length of pmnos cdna contained 3997 bp, includi ... | 2013 | 23994281 |
| molecular cloning, sequence analysis and expression of fein-penaeidin from the haemocytes of indian white shrimp fenneropenaeus indicus. | penaeidins are members of a special family of antimicrobial peptide existing in penaeid shrimp and play an important role in the immunological defense of shrimp. here, we report a penaeidin sequence cloned from the indian white shrimp fenneropenaus indicus (fein-penaeidin). the fein-penaeidin open reading frame encodes a 77 amino acid peptide including a 19 amino acid signal peptide. the deduced amino acid sequences of fein-penaeidin include a proline rich n-terminal domain and a carboxyl-domain ... | 2012 | 24371565 |
| isolation and expression analysis of an mapkk gene from fenneropenaeus chinensis in response to white spot syndrome virus infection. | mitogen-activated kinase kinase (mapkk) is an important gene involved in the host-virus interaction process. to obtain a better understanding of mapkk in the interaction process between the chinese shrimp fenneropenaeus chinensis and white spot syndrome virus (wssv), we cloned the sequence of an mapkk cdna from f. chinensis (fcmapkk) and investigated the effect of fcmapkk on wssv infection. the results showed that the fcmapkk gene contained a 1227 bp open reading frame (orf), which encoded a hig ... | 2016 | 27164214 |
| cloning of litopenaeus vannamei cd63 and it's role in white spot syndrome virus infection. | white spot syndrome virus (wssv) is currently the most serious shrimp pathogen, which has brought huge losses to shrimp industry worldwide. cd63 of shrimp belongs to the tetraspanin superfamily, which plays an important role in signal transduction and immune process. in this paper, cd63 cdna sequence of litopenaeus vannamei was cloned using race method. the amplified sequence is 1472 bp, with its orf 744 bp, encoding 247 amino acids. bioinformatics analysis showed that the sequence of lvcd63 has ... | 2016 | 26964710 |
| recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (fcalf5) from chinese shrimp fenneropenaeus chinensis. | antimicrobial peptides (amps) have a great potential to be used as a substitute for antibiotics since amps don't lead to bacteria's drug resistance. anti-lipopolysaccharide factors (alfs) are one type of amps and exist in crustaceans. in the present study, we produced a recombinant protein (rfcalf5) of an alf isoform (fcalf5) from chinese shrimp fenneropenaeus chinensis through a prokaryotic expression system. the rfcalf5 exhibited varied antibacterial activities against different bacteria. besi ... | 2015 | 26123888 |
| genomic organization and promoter analysis of a transcriptional repressor gene from fenneropenaeus chinensis. | in this study, we cloned and sequenced genomic sequences from a fenneropenaeus chinensis transcriptional repressor gene, fctr. the fctr gene is 2,671 bp in length and has four exons and three introns. the 873 bp promoter contains several transcription factor binding sites, including a tata box and a cyclic amp-responsive element. promoter deletion analysis using a luciferase reporter gene identified regulatory elements. challenge with white spot syndrome virus increased expression from the promo ... | 2015 | 25266240 |
| cloning and characterization of cytoplasmic dynein intermediate chain in fenneropenaeus chinensis and its essential role in white spot syndrome virus infection. | to investigate the role of cytoplasmic dynein in white spot syndrome virus (wssv) infection, the full-length cdna of cytoplasmic dynein intermediate chain (fcdynci) was cloned in fenneropenaeus chinensis, which consists of 2582 bp and encodes a polypeptide of 660 amino acids. sequence analysis and multiple sequence alignment displayed that fcdynci was a member of cytoplasmic dynein 1 family. the fcdynci mrna was most highly expressed in hemocytes, which was significantly up-regulated post wssv i ... | 2014 | 24925758 |
| the roles of β-integrin of chinese shrimp (fenneropenaeus chinensis) in wssv infection. | our previous study demonstrated that an integrin β subunit of chinese shrimp (fenneropenaeus chinensis) (fcβint) plays an important role in white spot syndrome virus (wssv) infection. in the present work, in order to further elucidate the potential role of fcβint in wssv infection, the recombinant extracellular domain of β integringene of f. chinensis (rfcβint-er) was expressed in escherichia coli bl21 (de3), and the eukaryotic expression plasmid pcdna3.1-fcβint-er (pfcβint-er) was also construc ... | 2017 | 28686185 |
| a new anti-lipopolysaccharide factor (alf) gene with its snp polymorphisms related to wssv-resistance of litopenaeus vannamei. | anti-lipopolysaccharide factors (alfs) of crustacean play an important role against bacteria or virus infection. in this study, the cdna sequence and genomic sequence of one new isoform of alf designated as nlvalf1 were reported. the open reading frame (orf) of nlvalf1 consisted of 369 bp encoding 123 amino acids and the genomic structure of nlvalf1 comprised four introns and three exons. the predicted pi of the deduced protein was 8.82 and the molecular weight (mw) was 13.72 kda. the deduced am ... | 2014 | 24769128 |
| the identification of micrornas involved in the response of chinese shrimp fenneropenaeus chinensis to white spot syndrome virus infection. | microrna (mirna) is a class of small noncoding rna, which is involved in the post-transcriptional regulation in all metazoan eukaryotes. mirnas might play an important role in the host response to virus infection. however, mirnas in the aquatic crustacean species were not extensively investigated. to obtain a better understanding of the response of chinese shrimp fenneropenaeus chinensis to white spot syndrome virus (wssv) infection, the sequence and expression profile of mirnas in the hepatopan ... | 2017 | 28554838 |
| cdna cloning and expression analysis of a phosphopyruvate hydratase gene from the chinese shrimp fenneropenaeus chinensis. | in the present study a cdna encoding a phosphopyruvate hydratase (enolase) was cloned from the muscle of the chinese shrimp (fenneropenaeus chinensis) and named as fcenolase. the cdna of fcenolase encoded a protein of 434 amino acid residues with a molecular mass 47.22 kda. the residues 342-355 constituted the signature motif "lllkvnqigsvtes". a snp locus (c96t) in the orf at 96 bp was identified. the results showed that the fcenolase was a conserved gene. in the normal f. chinensis, the mrna le ... | 2017 | 28216323 |
| comparative microarray profile of the hepatopancreas in the response of "huanghai no. 2" fenneropenaeus chinensis to white spot syndrome virus. | white spot syndrome virus (wssv) infects all shrimp species and is the greatest detriment to shrimp culture. to better understand the mechanism of molecular responses to wssv infection in "huanghai no. 2" fenneropenaeus chinensis, a microarray technique was used. microarray gene expression profiling of 59,137 unigenes identified differentially expressed genes (degs) both in live and moribund shrimp at early, peak and late phases. in live shrimp, 1307, 1479 and 1539 degs were obtained in the earl ... | 2016 | 27591045 |
| proteomic analysis of differentially expressed proteins in fenneropenaeus chinensis hemocytes upon white spot syndrome virus infection. | to elucidate molecular responses of shrimp hemocytes to white spot syndrome virus (wssv) infection, two-dimensional gel electrophoresis was applied to investigate differentially expressed proteins in hemocytes of chinese shrimp (fenneropenaeus chinensis) at 24 h post infection (hpi). approximately 580 protein spots were detected in hemocytes of healthy and wssv-infected shrimps. quantitative intensity analysis revealed 26 protein spots were significantly up-regulated, and 19 spots were significa ... | 2014 | 24587154 |
| cloning of hsp21 gene and its expression in chinese shrimp fenneropenaeus chinensis in response to wssv challenge. | in the present study, a dna sequence encoding a small heat shock protein gene (fchsp21) in the chinese shrimp, fenneropenaeus chinensis, was cloned, and its expression was analyzed after white spot syndrome virus (wssv) infection. the fchsp21 gene contained an open reading frame (orf) of 555 bp in length, encoding a 184 amino acid protein with a theoretical size of about 21 kda and a predicted isoelectric point of 5.38. the mrna of the hsp21 had a long poly(a) tail (748 bp) with six polyadenylat ... | 2014 | 24442516 |
| molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in fenneropenaeus chinensis and its antioxidant activity. | peroxiredoxins (prx) are a family of antioxidant proteins and perform important functions in intracellular signal transduction. here, we report a prx gene from chinese shrimp fenneropenaeus chinensis. the full-length cdna of fcprx gene contained an open reading frame of 735 bp encoding a polypeptide of 275 amino acids. the molecular mass of the deduced amino acid of fcprx is 27445.43 da with an estimated pi of 5.71. sequence comparison showed that the fcprx shares high identities with prx ivs an ... | 2014 | 24418456 |
| detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time pcr assay. | as one of the major pathogens, hepatopancreatic parvovirus (hpv) can cause severe diseases in penaeid shrimp. we developed a taqman-based real-time pcr assay for the hpv detection in china. a pair of primers (hpvf and hpvr) and a taqman probe were designed according to the hpv genomic sequence of chinese isolate (genbank: gu371276). our data showed that the primers and taqman probe were specific for hpv, and they exhibited no cross-reaction with infectious hypodermal and hematopoietic necrosis v ... | 2013 | 24090693 |
| identification, cloning and characterization of an extracellular signal-regulated kinase (erk) from chinese shrimp, fenneropenaeus chinensis. | extracellular signal-regulated kinase (erk) is a serine/threonine-specific protein kinase, which participates in signaling transduction pathways that control intracellular events, including resumption of meiosis, embryogenesis, cell differentiation, cell proliferation, cell death and response to radiation. some virus species evolved the ability to hijack the host cell erk signaling transduction pathway for viral replications and gene expressions. to obtain a better understanding of erk, we clone ... | 2013 | 24084041 |
| molecular cloning and characterization of a cathepsin b gene from the chinese shrimp fenneropenaeus chinensis. | cathepsin b is a unique member of the cathepsin superfamily, which acts as both an endopeptidase and peptidyl-dipeptidase. to obtain a better understanding of this enzyme, we cloned a cdna encoding cathepsin b from the muscle of fenneropenaeus chinensis (fccb). fccb contained a 996-bp open reading frame (orf) encoding a protein of 331 amino acid residues with a putative signal peptide and a propeptide_c1 at the n-terminal, a glutamine oxyanion hole and active site cysteine, histidine and asparag ... | 2013 | 24041842 |
| expression kinetics of β-integrin in chinese shrimp (fenneropenaeus chinensis) hemocytes following infection with white spot syndrome virus. | our previous study has demonstrated that an integrin β subunit of chinese shrimp (fenneropenaeus chinensis) (fcβint) involved in wssv infection. in order to further elucidate the potential role of the fcβint in the wssv infection, expression response of fcβint to wssv infection in shrimp hemocytes was investigated after intra-muscular injection with the virus. following time-course hemocytes sampling, the expression variation of fcβint in hemocytes was examined by flow cytometric immunofluoresce ... | 2013 | 23711468 |
| transcriptome analysis on chinese shrimp fenneropenaeus chinensis during wssv acute infection. | previous studies have discovered a lot of immune-related genes responding to white spot syndrome virus (wssv) infection in crustacean. however, little information is available in relation to underlying mechanisms of host responses during the wssv acute infection stage in naturally infected shrimp. in this study, we employed next-generation sequencing and bioinformatic techniques to observe the transcriptome differences of the shrimp between latent infection stage and acute infection stage. a tot ... | 2013 | 23527000 |
| genomic organization and promoter characterization of fcctl, a c-type lectin-like protein in fenneropenaeus chinensis. | diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. c-type lectins may play an important role in shrimp immunity. the gene encoding fcctl, a c-type lectin-like protein, was isolated from fenneropenaeus chinensis and characterized. this gene was composed of five exons spanning 2470 base pairs (bp). intron 1 contained a possible promoter and many potential transcription factor binding sites, such as those for tata binding protein, creb, srf, ttk and three sites f ... | 2013 | 23337594 |
| identification and molecular characterization of a c-type lectin-like protein from chinese shrimp (fenneropenaeus chinensis). | a c-type lectin-like protein was cloned and characterized from the chinese shrimp fenneropenaeus chinensis, and named as fcctl. the results indicated that the full length cdna of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved cys and one key motif epgd. the theoretical molecular weight and pi of mature protein was 25.3 kda and 5.4. sequence comparison of the deduced amino acid sequence of fcctl showed varied ident ... | 2013 | 23192616 |
| blocking the large extracellular loop (lel) domain of fctetraspanin-3 could inhibit the infection of white spot syndrome virus (wssv) in chinese shrimp, fenneropenaeus chinensis. | tetraspanins belong to the transmembrane 4 superfamily (tm(4)sf), which span the cell membrane 4 times and act as bridges or connectors. increasing evidences have shown that tetraspanins play important role in virus infection. the large extracellular loop (lel) of a tetraspanin is considered as a possible target of some virus. tetraspanins are widely found in invertebrates, but the functional roles of most invertebrate tetraspanins have remained unknown. recently, a tetraspanin, called fctetrasp ... | 2012 | 22406449 |
| expression profiles of the p38 mapk signaling pathway from chinese shrimp fenneropenaeus chinensis in response to viral and bacterial infections. | mitogen-activated protein kinase (mapk) signaling pathway plays an important role in cellular response to inflammatory cytokines, environmental stress and pathogenic infection, however, compared with mammals and insects, the potential function of p38 mapk from chinese shrimp (fenneropenaeus chinensis) in response to viruses and bacterial infection is limited. in the study, the immune responses of four genes mkk3, mkk4, p38 and atf-2 from f. chinensis were investigated in defending against white ... | 2018 | 29155327 |
| role of chymotrypsin-like serine proteinase in white spot syndrome virus infection in fenneropenaeus chinensis. | white spot syndrome virus (wssv) caused a great economic loss in shrimp aquaculture. although great efforts have been undertaken to characterize the virus disease during the last two decades, there are still lack of effective methods to prevent or cure it. in this study, we investigated the transcriptional expression profiles of 18 key immune-related genes in the chinese shrimp fenneropenaeus chinensis which was severely infected by wssv. we found that the expression levels of 6 genes including ... | 2013 | 23142726 |
| the vp37-binding protein f1atp synthase β subunit involved in wssv infection in shrimp litopenaeus vannamei. | to investigate the interaction between white spot syndrome virus (wssv)-vp37 and gill membrane proteins (gmps) of pacific white shrimp (litopenaeus vannamei), the vp37 protein was expressed and purified, and a distinct 53 kda vp37-binding protein band was identified in gmps by virus overlay protein binding assay and gst pull-down assay. by electroelution, the vp37 binding protein was purified and identified as f(1)atp synthase β (f(1)atpase β) subunit by mass spectrometry. the purified f(1)atpas ... | 2013 | 23108256 |