| molecular architecture of an n-formyltransferase from salmonella enterica o60. | n-formylated sugars are found on the lipopolysaccharides of various pathogenic gram negative bacteria including campylobacter jejuni 81116, francisella tularensis, providencia alcalifaciens o30, and providencia alcalifaciens o40. the last step in the biosynthetic pathways for these unusual sugars is catalyzed by n-formyltransferases that utilize n(10)-formyltetrahydrofolate as the carbon source. the substrates are dtdp-linked amino sugars with the functional groups installed at either the c-3' o ... | 2017 | 28263875 |
| pathogenic providencia alcalifaciens strain that causes fatal hemorrhagic pneumonia in piglets. | investigation of a serious pig disease with high mortality and typical lung lesions yielded a bacterial isolate identified as providencia alcalifaciens based on the 16s ribosomal dna sequence analysis. the pathogenicity of this bacterial isolate was confirmed in piglets and mice. the bacterial strain caused the typical illness in piglets, which suffered serious dyspnea and hemorrhagic pneumonia. the drug resistance spectrum of the bacterium was also determined. the results indicated that the iso ... | 2014 | 24129837 |
| new role for the ankyrin repeat revealed by a study of the n-formyltransferase from providencia alcalifaciens. | n-formylated sugars such as 3,6-dideoxy-3-formamido-d-glucose (qui3nfo) have been observed on the lipopolysaccharides of various pathogenic bacteria, including providencia alcalifaciens, a known cause of gastroenteritis. these unusual carbohydrates are synthesized in vivo as dtdp-linked sugars. the biosynthetic pathway for the production of dtdp-qui3nfo requires five enzymes with the last step catalyzed by an n-formyltransferase that utilizes n(10)-tetrahydrofolate as a cofactor. here we describ ... | 2015 | 25574689 |
| cloning and characterization of a new ribitol dehydrogenase from providencia alcalifaciens rimd 1656011. | a new ribitol dehydrogenase gene was cloned from providencia alcalifaciens rimd 1656011 and expressed in escherichia coli bl21. this study aimed to purify and characterize the ribitol dehydrogenase from p. alcalifaciens rimd 1656011 and investigate its substrate specificity for potential use as an industrial enzyme. | 2016 | 26693956 |
| providencia alcalifaciens causes barrier dysfunction and apoptosis in tissue cell culture: potent role of lipopolysaccharides on diarrheagenicity. | providencia alcalifaciens is a member of the enterobacteriaceae family that occasionally causes diarrheagenic illness in humans via the intake of contaminated foods. despite the epidemiological importance of p. alcalifaciens, little is known about its pathobiology. here we report that p. alcalifaciens causes barrier dysfunction in caco-2 cell monolayers and induces apoptosis in calf pulmonary artery endothelial cells. p. alcalifaciens infection caused a 30% reduction in transepithelial resistanc ... | 2013 | 23710559 |
| enterobacterial detection and escherichia coli antimicrobial resistance in parrots seized from the illegal wildlife trade. | enteric bacteria are considered important potential pathogens in avian clinical medicine, causing either primary or opportunistic infections. the aim of this study was to evaluate the frequency of enterobacteria in the intestinal microbiota of psittacine birds and to determine the antimicrobial susceptibility of the escherichia coli isolates cultured. fecal samples were collected from 300 parrots captured from the illegal wildlife trade in goiás, brazil and were processed using conventional bact ... | 2013 | 23505696 |
| structure and gene cluster organization of the o-antigen of providencia alcalifaciens o45:h25. | o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o45:h25 and studied by sugar analysis, smith degradation, and (1)h and (13)c nmr spectroscopy. the following structure of the pentasaccharide repeat of the o-polysaccharide was established: [structure: see text]. the o-antigen gene cluster of p. alcalifaciens o45 was sequenced and found to be in full agreement with the o-polysaccharide structure established. | 2014 | 25240185 |
| isolation and selection of new biosurfactant producing bacteria from degraded palm kernel cake under liquid state fermentation. | biosurfactants are surface-active compounds produced by different microorganisms. the aim of this study was to introduce palm kernel cake (pkc) as a novel substrate for biosurfactant production using a potent bacterial strain under liquid state fermentation. this study was primarily based on the isolation and identification of biosurfactant-producing bacteria that could utilize palm kernel cake as a new major substrate. potential bacterial strains were isolated from degraded pkc and screened for ... | 2014 | 25007747 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o33. | mild acid degradation of the lipopolysaccharide from providencia alcalifaciens o33 resulted in an o-polysaccharide along with core and o-unit-bearing core oligosaccharides. composition of the oligosaccharides was inferred by esi mass spectrometry. based on sugar and methylation analyses, smith degradation and (1)h and (13)c nmr spectroscopy data, the following structure of the tetrasaccharide o-unit of the o-polysaccharide was established: another o-polysaccharide structure has been reported ear ... | 2014 | 24727107 |
| structure of the o-polysaccharide of providencia alcalifaciens o35 containing an n-[(s)-1-carboxyethyl]-l-alanine (alanopine) derivative of 4-amino-4,6-dideoxyglucose. | the o-polysaccharide of providencia alcalifaciens o35 was studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(13)c hmbc, and noesy experiments in d2o and, to detect correlations for nh protons, in a 9:1 h2o/d2o mixture. a unique n-(1-carboxyethyl)alanine (alanopine, alo) derivative of 4-amino-4,6-dideoxyglucose (qui4n) was identified as the polysaccharide component. alanopine was isolated by solvolysis of the polysaccharide with triflic acid f ... | 2013 | 23694707 |
| structural characterization of enzymatic products in the dtdp-d-qui4nfo biosynthetic pathway using electrospray ionization tandem mass spectrometry. | structural characterization of biosynthetic precursors is very important in assigning enzymatic function to proteins that have been identified as functional homologs on the basis of sequence homology alone. the objective of this study is to demonstrate the use of electrospray ionization tandem mass spectrometry (esi-ms/ms) as a powerful technique for the characterization of enzymatic products in the biosynthetic pathway of deoxythymidine 5'-diphosphate-4-formamido-4,6-dideoxy-d-glucose (dtdp-d-q ... | 2013 | 23418147 |
| structural, serological, and genetic characterization of the o-antigen of providencia alcalifaciens o40. | the o-polysaccharide chain of the lipopolysaccharide (o-antigen) on the bacterial cell surface is one of the most structurally variable cell components and serves as a basis for serotyping of gram-negative bacteria, including human opportunistic pathogens of the genus providencia. in this work, the o-antigen of providencia alcalifaciens o40 was obtained by mild acid degradation of the isolated lipopolysaccharide and studied by chemical methods and high-resolution nmr spectroscopy. the following ... | 2012 | 23163869 |
| lead-resistant providencia alcalifaciens strain 2ea bioprecipitates pb+2 as lead phosphate. | a lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as providencia alcalifaciens based on biochemical characteristics, fame profile and 16s rrna sequencing and designated as strain 2ea. it resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insoluble light brown solid. scanning electron microscopy coupled with energy-dispersive x-ray spectrometric analysis (sem-edx) and x-ray diffraction spectroscopy (xrd) revealed extracellul ... | 2013 | 23163530 |
| structure of the o-polysaccharide of providencia alcalifaciens o3 containing 3,6-dideoxy-3-formamido-d-glucose and d-galacturonamide. | mild acid degradation of the lipopolysaccharide (lps) of providencia alcalifaciens o3 followed by gpc on sephadex g-50 and anion-exchange chromatography on deae-trisacryl m afforded neutral and acidic polysaccharides, lps core oligosaccharide, and an oligosaccharide composed of one repeat of the neutral polysaccharide (o-unit) linked to the lps core. the following structure of the pentasaccharide o-unit was established by sugar and methylation analyses, 2d (1)h and (13)c nmr spectroscopy and esi ... | 2012 | 22960211 |
| structure of a peptidoglycan-related polysaccharide from providencia alcalifaciens o45. | a polysaccharide was isolated from the opportunistic human pathogen providencia alcalifaciens o45:h26 by extraction with aqueous phenol and studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including two-dimensional roesy and h-detected (1)h,(13)c hsqc experiments. the polysaccharide contains n-acetylglucosamine and n-acetylmuramic acid (d-glcpnac3rlac) amidated with l-alanine and has the following structure: →4)-β-d-glcpnac-(1→4)-β-d-glcpnac3(rlac-l-ala)-(1→. ... | 2012 | 22817460 |
| genetic analysis of the o-antigen of providencia alcalifaciens o30 and biochemical characterization of a formyltransferase involved in the synthesis of a qui4n derivative. | o-antigen is a component of the outer membrane of gram-negative bacteria and one of the most variable cell surface constituents, giving rise to major antigenic variability. the diversity of o-antigen is almost entirely attributed to genetic variations in o-antigen gene clusters. bacteria of the genus providencia are facultative pathogens, which can cause urinary tract infections, wound infections and enteric diseases. recently, the o-antigen gene cluster of providencia was localized between the ... | 2012 | 22661447 |
| localization and molecular characterization of putative o antigen gene clusters of providencia species. | enterobacteria of the genus providencia are opportunistic human pathogens associated with urinary tract and wound infections, as well as enteric diseases. the lipopolysaccharide (lps) o antigen confers major antigenic variability upon the cell surface and is used for serotyping of gram-negative bacteria. recently, providencia o antigen structures have been extensively studied, but no data on the location and organization of the o antigen gene cluster have been reported. in this study, the four p ... | 2012 | 22282517 |
| structure of the o-polysaccharide of providencia alcalifaciens o2 containing ascarylose and n-(l-alanyl)-d-glucosamine. | the o-polysaccharide was obtained by degradation of the lipopolysaccharide of providencia alcalifaciens o2 under mild acidic conditions followed by gpc. the polysaccharide was found to contain two unusual components: 3,6-dideoxy-l-arabino-hexose (ascarylose, asc) and 2-(l-alanyl)amino-2-deoxy-d-glucose (glcnala). ascarylose was partially split off during lipopolysaccharide degradation and could be eliminated completely by selective acid hydrolysis, which also partially cleaved the β-galnac-(1 → ... | 2015 | 25464076 |