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substance p: model studies of its binding to phospholipids.1. the partition of substance p (sp) between buffer solutions (ph 1.6--7.8) and an organic, phospholipid (phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidyl choline) containing phase (chloroform:methanol 2:1) was studied. 2. the binding of sp to phosphatidyl serine, phosphatidyl ethanolamine and phosphatidyl inositol was lowest at ph 2 and increased with ph. the binding to phosphatidyl choline was much smaller and less dependent on ph. 3. in contrast to the ba ...197938406
bioconversion of wheat straw and wheat straw components into single-cell protein.several fungi (aspergillus niger, a. terreus, cochliobolus specifer, myrothecium verrucaria, rhizoctonia solani, spicaria fusispora, penicillium sp., and gliocladium sp.) were isolated from decomposing wheat straw and tested for their ability to utilize whole straw and its components, holocellulose (hemicellulose and cellulose) and cellulose, for the production of single-cell protein (scp). it was found that c. specifer was the most efficient fungus for protein synthesis with the three substrate ...197938899
purification and some properties of two nadp+-specific isocitrate dehydrogenases from an obligately psychrophilic marine bacterium, vibrio sp., strain abe-1.two isozymes of nadp+-specific isocitrate dehydrogenase [icdh; ec 1.1.1.42] were confirmed to be present in an obligately psychrophilic marine bacterium, vibrio sp., strain abe-1, on the basis of the temperature-activity curve and electrophoretic mobilities. these isozymes were separated and purified about 170-fold for isozyme i (specific activity at 40 degrees c, 24.3 units/mg protein) and about 180-fold for isozyme ii (specific activity at 20 degrees c, 59.2 units/mg protein), though the isozy ...197939069
manganese oxidation by an intracellular protein of a pseudomonas species.cultures of a pseudomonas sp. strain mnb 1 produce an intracellular, manganese oxidizing protein (abbrev. as mn ox. protein) during the stationary phase of growth. this protein is heat labile, can be inactivated by protease and has a ph-optimum for manganese oxidation at ph 7.0. mn2+ is oxidized only at concentrations below 3-10(-5) m. the occurrence of the protein is not dependent on the presence of mn2+, but is clearly related to the cessation of growth after the end of the exponential growth ...197939383
the purification of glutamine synthetase from azotobacter and other procaryotes by blue sepharose chromatography.we report the facile purification of glutamine synthetase (l-glutamate: ammonia ligase (adenosine 5'-diphosphate-forming), ec 6.3.1.2) in both the adenylylated and unadenylylated form, from azotobacter vinelandii atcc 12837. a general affinity column, which used as an affinity ligand reactive blue 2 dye (cibacron blue) covalently linked to agarose, was employed as an efficient first step of purification. further purification to electrophoretic homogeneity employed deae-cellulose chromatography a ...197939606
isolation, characterization and the role of rabbit testicular arysulphatase a in fertilization.arysulphatase a was purified from rabbit testis. the purification was accomplished by a four-step procedure involving (nh4)2so4 fractionation, chromatography on deae-cellulose, sp(sulphopropyl)-sephadex and affinity chromatography on concanavalin a-sepharose. the specific activity of purified preparation was 135 mumol/min per mg of protein, which represented an increase of 900-fold above that of the crude homogenate. the purified enzyme (20-50 micrograms) was found to move electrophoretically as ...197940545
carbon dioxide assimilation in cyanobacteria: regulation of ribulose, 1,5-bisphosphate carboxylase.cyanobacteria assimilate carbon dioxide through the calvin cycle and therefore must regulate the activity of ribulose 1,5-bisophosphate carboxylase. using an in situ assay, as well as measuring the activity in crude, partially purified, and homogeneous preparations, we can show that a number of phosphorylated intermediates exert a regulatory role. three diverse organisms, agmenellum quadruplicatum, aphanocapsa 6714, and anabaena sp. ca, were studied, and it was found that the in situ and cell-fr ...197940958
eastern equine encephalomyelitis virus in experimentally infected bats.colonial bats (myotis supp. and eptesicus sp.) were infected with eastern equine encephalomyelitis virus by subcutaneous inoculation or by the bite of infected mosquitoes. bats were maintained in an environment simulating conditions encountered in hibernacula or in summer maternal colonies. virus was detected in the blood of hibernating bats at irregular intervals over a 42-day observation period; viremia perhaps was influenced by the amount of disturbance (arousal) involved in the blood samplin ...197941109
[breakdown of luliberin, somatostatin and substance p as an effect of hypothalamic endopeptidases].acid and neutral proteinases were isolated with the purpose of investigating their participation in the breakdown of hypothalamic peptides and proteins. the acid proteinase was purified about 1000-fold from hypothalamus by precipitation with acetone, chromatography on sp-sephadex g-50, gel filtration through column of g-100 and chromatography on deae-sephadex a-50. the molecular weight of the enzyme was approximately 50.000. maximal activity against hemoglobin was obtained at ph 3,2--3,5: serum ...197841363
effect of substance p on cck- or vip-induced choleresis in anesthetized dogs.10 anesthetized dogs were provided with acute common bile duct fistulas and the gallbladder was excluded. hepatic bile output and biliary content of sodium, potassium and amylase were studied. 6 caval infusions were administered of cck, 0.3 ivy u.kg-1.min-1, with a superimposed infusion of sp, 20 ng.kg-1.min-1. 7 caval infusions were given of vip, 50 ng.kg-1.min-1, with a superimposed infusion of sp, 20 ng. kg-1.min-1. cck increased bile output and biliary content of sodium, potassium and amylas ...197941410
anaerobic isolates in chronic recurrent suppurative otitis media. treatment with carbenicillin alone and in combination with gentamicin.tympanocentesis was performed in 32 pediatric patients with chronic recurrent suppurative otitis media. the aspirate was cultured aerobically and anaerobically. aerobes were isolated from ten patients (31.2%); anaerobes from one patient; and both aerobes and anaerobes from 21 patients (65.6%). there were 46 aerobic isolates. the aerobes commonly recovered were pseudomonas aeruginosa (24 isolates) proteus sp. (5) and staphylococcus aureus (3). there were 32 anaerobes isolated including anaerobic ...197941811
the parasitic coelenterate, polypodium hydriforme ussov, from the eggs of the american acipenseriform polyodon spathula.no significant differences in macro- and micromorphology were found between the parasitic stolon and free-living polyps of polypodium sp. obtained from infected eggs of the north american acipenseriform fish polyodon spathula and corresponding developmental stages of polypodium hydriforme ussov, parasitic in the volga sterlet (acipenser ruthenus). therefore, both the american and the european forms of polypodium belong to the species p. hydriforme ussov.197941895
isolation and properties of the protease from the wild-type and mutant strains of pseudomonas fragi.a simplified procedure for the purification of the extracellular protease of pseudomonas fragi was developed. the enzyme was isolated from a derepressed mutant producing 40 times the enzyme level of the parental organism. it was collected from culture filtrates by ammonium sulfate precipitation, and it was obtained in pure form by single chromatography on a column of diethylaminoethyl cellulose. the protease had a molecular weight of 52,000 as estimated by sodium dodecyl sulfate-gel electrophore ...197942639
identification of colonies of moraxella bovis by plate epi-immunofluorescence.a modification of the plate epi-immunofluorescence test was found to be a reliable method for the positive identification of colonies of moraxella bovis when fluorescein isothiocyanate-conjugated globulin prepared against pili from selected strains of m bovis was used. a cross-reaction with other members of the genus moraxella or other moraxella-like organisms was not observed, except with an equine moraxella sp, which produced a weak reaction.197943106
the effect of sulfasalazine and its active components on human polymorphonuclear leukocyte function in relation to ulcerative colitis.sulfasalazine and its active components, 5-aminosalicylic acid (5-asa) and sulfapyridine (sp), are potent modulators of inflammatory reactions but with somewhat different modes of action. investigating the effect of these compounds on normal human polymorphonuclear leukocytes in vitro, we show inhibition of different stages in the phagocytic process, such as migration (sulfasalazine and sp), superoxide production (sulfasalazine and sp), myeloperoxidase-mediated iodination and cytotoxicity (5-asa ...197943662
[purification and physico-chemical properties of collagenase synthesized by a bacterium of the type acinetobacter sp].the acinetobacter spec collagenase has been almost completely purified. this enzyme is a true collagenase the activity of which is high on collagen. the enzyme is active on insoluble collagen, gelatin and the synthetic pz-peptide, but has no proteolytic activity on casein or bovine serum-albumin. the collagenase was obtained on a simple medium with gelatin and yeast extract. the enzyme was purified by (nh4)2so4 precipitation. deae cellulose column chromatography, sephadex g 200 gel-filtration. t ...197943744
interaction of arylsulphatase-a with sp-sephadex. a note on the isolation of arylsulphatase-a from human urine. 197943867
acid hydrolases of trypanosomatid flagellates.homogenates from 5 species of trypanosomatids were screened for the presence of a series of acid hydrolases. the insect flagellae, crithidia sp., contains 5 enzymes reminiscent of plant parasitism, which were absent from or of very low specific activity in parasites of the genera, trypanosoma and leishmania. the latter mammalian parasites, on the other hand, exhibited higher acid proteinase and alpha-d-mannosidase activity levels.197944097
parasites in bulinus senegalensis (mollusca: planorbidae) and their detection.isoelectric focusing studies on enzyme variation between populations of the snail bulinus senegalensis revealed that parasitic infections in the snails contributed additional bands of enzyme activity, particularly in the glucose phosphate isomerase (gpi) and malate dehydrogenase (mdh) systems. the patterns due to the parasite enzymes were, in most cases, clearly distinct from those of the host and different from each other. parasites encountered included schistosoma haematobium, s. bovis, paramp ...197944358
summary of laboratory studies on the antibacterial activity of cefaclor.laboratory aspects of cefaclor, a new orally-effective cephalosporin antibiotic, are summarized. on the basis of data from a variety of studies, the useful antibacterial spectrum of cefaclor is shown to include all classes of bacteria that are generally susceptible to cephalothin and cephalexin. cefaclor has a significant potency advantage over cephalexin against many enterobacteriaceae, haemophilus sp. and streptococcus pneumoniae. bacteria that are susceptible to cefaclor are killed by concent ...197945088
effect of cardiotonic and vasoactive drugs on transmural flow distribution and ventricular volume in the fibrillating heart on cardiopulmonary bypass.subendocardial hemorrhagic necrosis in an important cause of death following cardiopulmonary bypass. the transmural distribution of flow across the left ventricle (lv), septum (sp), and right ventricle (rv) is a complex interaction of vascular resistance and myocardial compressive resistance. we studied the change in transmural blood flow in lv, sp, and rv, and left ventricular volume, following administration of cardiotonic and vasoactive drugs in the fibrillating heart. the drugs studied inclu ...197545821
an injury-induced diffuse slow potential from brain.three different slow potential (sp) changes resulting from focal brain injury are described. the first is an immediate, high amplitude (in excess of 25 mv)negative shift at the site of injury. the second is a biphasic negative-positive sp wave which spreads throughout the cortex ipsilateral to injury and is similar to spreading depression (sd). the third sp change, called here the injury-induced diffuse slow potential (idsp)is a prolonged (lasting approx. 2h) negative shift occurring simultaneou ...197546817
primary interaction between antibody and components of alternaria. i. immunology and chemical characteristics of labeled antigens.components were isolated from alternaria tenuis and its culture filtrate, and were radiolabeled with 123-i. the labeled antigenic components had a high polysaccharide content as determined by staining patterns following electrophoresis in polyacrylamide gels and by inactivation with sodium metaperiodate. a primary binding test was employed to detect and measure serum antibodies to the components from a. tennuis. this procedure was more sensitive in detecting antibodies that bound to antigens tha ...197548526
[the differenziation of swine-mycoplasma by acrylamide-gel-electrophoresis (author's transl)].the strains of swine mycoplasma (tab. 1) were grown aerobically in whittlestone's medium containing swine serum for 4-5 days at 37 degrees c. after centrifugation and washing they were freeze-dried and extracted with a phenol-acetic acid-water solution (4:2:1) (1 ml/5 mg mycoplasma dru weight) at 4 degrees c over 48 h. after centrifugation 2 volumes of the supernatant were mixed with 1 volume of a 40% sucrose solution in 35% acetic acid. 0,15 or 0,2 ml of which were added to the acrylamide-gel ( ...197549991
variants of n-tropic leukemia virus derived from balb/c mice.clonal lines derived from cultures of nih/3t3 cells infected with n-tropic leukemia virus from balb/c mice differ in the amount and type of n-tropic virus they produce. three biologically distinguishable n-tropic viruses were found: the large xc plaque-forming virus of hartley et al. (1969) (lp-n), a small xc plaque-forming virus (sp-n), and a non-plaque-forming virus (np-n). sp-n and np-n are less infectious than lp-n. upon prolonged passage in nih/3t3 cells np-n gives rise to highly infectious ...197551938
immunological relationships of bacterial l-asparaginases.rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...197654404
glycoproteins in membranes of secretory granules of the anterior pituitary gland.rat anterior pituitary glands were examined by electron microscopy after staining with five different histochemical stains. histochemical reactions were observed in the cell coat, cell membrane and the membrane surrounding the secretory granules in all anterior pituitary cells following staining with phosphotungstic acid (pta), chromic acid and pta, the periodic acid-thiosemicarbazide-silver protein method (pa-tsc-sp) of thiéry, ruthenium red and concanavalin a. the staining was abolished when t ...197655405
parital characterization of the rat anti-encephalitogenic protein (rscp).a protein antigenically similar to the anti-encephalitogenic bovine spinal cord protein (bscp) was detected in saline extracts of rat nervous tissues by immunodiffusion analyses using a rabbit anti-bscp serum. rat scp (rscp) appears to be evenly distributed throughout all parts of the rat nervous system and occurs also in the thymus, thyroid, and adrenal glands. although immunodiffusion analyses indicated that rscp shares some antigenic sites with bscp, anti-rscp sera reacted only with rscp, ind ...197655440
regulation of slow potential shifts in nucleus reticularis thalami by the mesencephalic reticular formation and the frontal granular cortex.novel stimuli or electric stimulation of the mesencephalic reticular formation (mrf) produced large positive slow potentials (sps) in rostral nucleus reticularis thalami (rva) that accompanied the negative sps known to occur in frontal cortex. sp durations (20-30 sec) were similar to the periods of unit inhibition that occur in rva following mrf stimulation. trains of 8 c/sec medial thalamic stimuli produced phasic negative sps in rva similar in duration to the intervals of unit excitation that ...197657857
utilization and degradation of lindane by soil microorganisms.of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. thirteen soil microorganisms were selected to study the utilization of lindane. lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachloro-1-cyclohexene (gamma-pcch), alpha-3,4,5,6-tetrachloro-1-cyclohexene (alpha-tcch), beta3,4,5,6-tetrachloro-1-cyclohexene (beta-tcch), gamma-3,4,5,6-tetrachloro-1-cyclohexene (gamm ...197660090
bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties.cells of pseudomonas putida np, pseudomonas species (ncib 9816), and a nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (nad+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. similarly, cells of a strain of p. putida biotype a, when grown either on toluene or benzene vapors, were found to contain a dehydrogenase that oxidized dihydrodiols of aromatic hydrocarbons with cis ste ...197662750
[heterogeneity of l-valine pool in actinomyces sp. 26-115 producing actinomycin c].free intracellular valine in actinomyces sp. 26-115 producing actinomycin c its functionally heterogenous. there are at least 2 pools of free valine. one of them supplies valine for protein biosynthesis and the second for the antibiotic biosynthesis. the volume of the pools is estimated. inadequate reaction of the pools to similar effects is indicative of differences in their properties. the pool participating in protein biosynthesis strives for preservation of its volume. the pool participating ...197666908
the action of lithium carbonate on the sleep-waking cycle in the cat.the therapeutic effect of lithium carbonate on manic-depressive psychosis is now universally recognized. in view of this positive action on the cyclic endogenous manifestations, it was interesting to investigate the possible effects of lithium on the sleep-waking cycle in the cat. polygraphic recording from three adults cats was carried out during 24 h periods before, and on different days after, beginning treatment with lithium carbonate. with low doses (30 and 50 mg/kg/day), important morpholo ...197767027
sucrose-negative variants of candida tropicalis.four cultures of a candida sp. that lacked alpha-glucosidase activity were isolated from clinical specimens. physiological, morphological, and serological characterizations of the yeasts and deoxyribonucleic acid reassociation studies supported their classification as a variant of c. tropicalis.197767125
characterization of human papovavirus rfv: comparison with sv40 and bkv.human papovavirus, rfv, isolated from urine of a renal transplant patient was compared with two strains of sv40 and with the prototype human papovavirus, bkv. neutralization tests showed that rfv and bkv are indistinguishable, while large-plaque (lp) and small-plaque(sp) isolates of sv40 gave a low but significant level of cross-reaction with rabbit or human antisera against rfv. dna reassociation saturation tests using 125i-labelled rfv dna show that bkv has 88% homology, and sp-sv40 has 29% ho ...197767183
specific inhibition of viral neuraminidases by an inhibitor, neuraminin, produced by streptomyces sp. 197767704
formylation and acetylation of 4-chloroaniline by a streptomyces sp.4-chloroaniline was metabolized in a liquid growth medium by a streptomyces sp. which was isolated from soil. after 60 gours of incubation the aniline had disappeared and several metabolites could be detected by thin layer chromatographic analysis. 4-chloroformylaniline and 4-chloroacetanilide were identified as products. the formation of a formylanilide by the actinomycete indicates a new mechanism of microbial aniline transformation.197767755
demonstration of intracytoplasmic glycogen of megakaryocytes and blood platelets by means of the periodic acid-thiocarbohydrazide-silver proteinate method.the glycogen of megakaryocytes and blood platelets has been investigated in glutaraldehyde and osmium tetroxide fixed tissues by the periodic acid-thiocarbohydrozide-silver proteinate method (pa-tch-sp). the pa-tch-sp method involves the staining of intracytoplasmic glycogens more densely than the routine lead citrate method. glycogen having a mean particle diameter of 21.1 nm has been shown localizing in the matrix of mature megakaryocytes, while that of glycogen in the platelets was 26.2 nm. t ...197769618
synthesis of cellulase by fusarium sp. in different culture conditions.the mechanism of the synthesis of cellulases by fusarium sp. strain was studied. it was found that a significant role in determination of cellulases is played by the adsorption of these enzymes on cellulose. the aeration level of the culture media had a significant effect on the synthesis as well as on the enzymatic complexity of cellulases.197770972
development and evaluation of an immunodiffusion test for diagnosis of systemic zygomycosis (mucormycosis): preliminary report.an antigen analysis with filtrate and homogenate precipitinogens of single isolates of the zygomycetes absidia corymbifera, mucor pusillus, rhizopus arrhizus, and rhizopus oryzae demonstrated the presence of common antigens among the three genera as well as antigens which permit their differentiation. selected homogenate antigens were valuable in developing a diagnostic immunodiffusion (id) test for systemic zygomycosis. when sera from 43 patients with various proven mycoses other than zygomycos ...197875212
humic-like substances of bacterial origin. i. some aspects of the formation and nature of humic-like substances produced by pseudomonas.two bacterial strains pseudomonas acidovorans no 26 and pseudomonas sp. no 4 grown in conn and yeast extract-glucose media, or in the media enriched with tyrosine, were found to produce dark brown pigment. it was shown that in the bacterial cultures numerous phenolic and quinone-type compounds were formed and transformed to humic-like polymers. formation of humic-like substances started in the bacterial cells and was accompanied by the presence of phenyloxidases in the bacterial cultures. the ba ...197775666
immunodiffusion studies on schistosoma mansoni and its intermediate host stage specific antigens. 3. immunoelectrophoresis cross-reactions between s. mansoni stages and biomphalaria sp. hepato-pancreas antigens.immunoelectrophoresis was applied for cross-wise checking of the different stages in the life cycle of schistosoma mansoni with extracts of hepato-pancreas from its intermediate host snails biomphalaria glabrata and biomphalaria alexandrina. only few fractions were common to non infected snail organ and parasite development stage, while more crossreactions were detected with infected snail hepato - pancreas.197877220
bovine fetus-specific serum proteins. purification and characterization of alpha1-fetoprotein and immunological identification of alpha2- and beta-fetoproteins.bovine alpha1-fetoprotein was isolated from fetal calf serum by successive procedures of concanavalin a-sepharose chromatography, deae-sephadex chromatography, sp-sephadex chromatography and preparative disc polyacrylamide gel electrophoresis. the bovine alpha1-fetoprotein preparation was considered homogeneous by several physicochemical and immunochemical criteria. bovine alpha1-fetoprotein has a molecular weight of 68 000 with an amino acid composiotn similar to that of other mammalian alhpa1- ...197878727
bacterial decomposition of synthetic 14c-labeled lignin and lignin monomer derivatives.nocardia sp. which was isolated from soil is capable of degrading synthetic lignin and utilizing its monomer derivatives. decomposition was monitored by measuring the 14co2 evolved and o2 consumed, when the bacterium was grown on a medium containing specifically 14c-labeled ligning or monomer phenolic compounds as major carbon source. the time course of the 14co2 release and o2 uptake indicates a significant depolymerization and utilization of lignin by the nocardia sp.197880922
detection of goitrin and its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture, electrolytic conductivity and sulfur detectors.the separation of goitrin by two gas-liquid chromatographic columns, the response of different detectors and the use of the heptafluorobutyric (hfb) anhydride derivative of goitrin to improve sensitivity was investigated. the non-polar 3% ov-1 and the intermediate 4% se-30/6% sp-2401 on 80-100 mesh chromosorb w hp gave comparable results and were considered interchangeable. the sensitivities of the electron capture, sulfur 394 nm emission, chlorine and nitrogen coulson electrolytic conductivity ...197881212
effect of various nitrogen salts in beet pulp medium on polygalacturonase activity of penicillium sp. 7/4b/ei 1 mutant.the highest pg activity was obtained in beet pulp with (nh4)2hpo4 in amount equivalent to 0.14--0.28% n after 7 days of growth; at optimal concentration, (nh4)2hpo4 did not influence the biomass production, but increased the ph of the culture media to above 6.0. the pg was an extracellular enzyme, acting mainly on highly esterified pectin. the attack was of a random manner, characteristic for the endo-enzymes. the enzyme was active at temperature 20 degrees c--40 degrees c and ph 5.0--6.0.197881597
rapid diagnosis of bacteroides infections by indirect immunofluorescence assay of clinical specimens.43 specimens from a variety of sites were directly examined by indirect immunofluorescence assay (i.f.a.) with specific antisera against the capsular polysaccharide of bacteroides fragilis and pooled antisera against a number of serotypes of bacteroides sp. (all of the former b. fragilis subspecies). the findings were compared with those of routine anaerobic bacteriology and gas liquid chromatography for short chain fatty acids. examination by i.f.a. was a sensitive (100%) and specific (90.3%) m ...197984899
substance p in the vagus nerve. immunochemical and immunohistochemical evidence for axoplasmic transport.1. the presence of immunoreactive substance p (i-sp) in the vagus nerve of 5 species was demonstrated by radioimmunoassay. different amounts of sp per unit weight were found: guinea pig greater than cat greater than rabbit, rat and cattle. 2. infranodose ligations of the vagus nerve of cats and rabbits caused an accumulation of i-sp proximal but not distal to the ligation. the results obtained by radioimmunoassay and immunohistochemistry indicate a somatofugal axoplasmic transport of sp. 3. doub ...197985263
[aspects of the biosynthesis of actinomycin c].the protoplasts of actinomyces sp. 26--115 producing actinomycin c were obtained by the action of lysozyme on the mycelial paste of a 48-hour microbial culture. the protoplast capacity for synthesizing actinomycin was decreased as compared to that of the intact mycelium. the transport of l-isoleucine, a precursor of actinomycin c biosynthesis in the protoplasts also decreased but this could not be the only cause of the decrease in the actinomycin biosynthesis capacity. the biosynthesis of actino ...197987146
ultrastructural localization of sulfated complex carbohydrates with a modified iron diamine procedure.a thiocarbohydrazide-silver proteinate (tch-sp) sequence was applied to thin sections of specimens that had been reacted with the high iron diamine (hid) method for ultrastructural localization of sulfated complex carbohydrates. the exposure to tch-sp enhanced the electron opacity of hid-reactive sites and increased the sensitivity of the procedure. this held true for hid-reacted specimens whether or not they had been post-treated with osmium tetroxide. however in those not postosmicated after h ...197989157
strain-specific antigens in rhizobium leguminosarum.fifty strains of rhizobium leguminosarum, isolated from five species of host plant (pisum sativum, p. arvense, vicia sativa, v. faba, and a lathyrus sp.) were examined for the presence of strain-specific somatic antigens by immune-diffusions against 13 antisera. thirty eight strains (76 per cent) were found to belong to the same sero-group and were serologically indistinguishable from each other, but four of these strains also exhibited non-reciprocal cross reactivity with other antisera. in con ...197989766
[ultrastructure of mycoplasma sp. demonstrated by phosphotungstic acid-chromic acid ph 0,4].ultrastructural observation of mycoplasma sp. revealed by the phosphotungstic acid-cromic acid, ph 0,4. arq. inst. biol., são paulo, 45(3) :169-176, 1978. cells of ib-rs-2 (clone c-26-3) swine line were examined under electron microscopy, employing the differential coloration with pta-acr 5 %, ph 0,4, during 30 minutes, at 37 degrees c. through this method we could observe the intracitoplasmatic forms, electronically dense, rather pleomorfic, with sizes varying between 60nm and 640nm and a unita ...197889840
[isolation of the membranes of actinomyces sp. 26-115, a producer of actinomycin c].a summation fraction of the membranes of actinomyces sp. 26-115 was obtained as a result of lysis of its protoplasts in a hypotonic medium. the qualitative content of protein, lipids, phospholipids, nucleic acids, glucosamine and muramic acid was determined in the membranes at various stages of the organism development. phosphatidylcholine is the main component of phospholipids in this organism. intracellular actinomycin was found inside the protoplasts. electrophoregrams of the microprotoplasts ...197990478
ultrastructural identification of non-adrenergic, non-cholinergic nerves in the rat anococcygeus muscle.the innervation of the rat anococcygeus muscle has been investigated ultrastructurally following fixation with a modified chromaffin reaction for the demonstration of biogenic amines (tranzer and richards, 1976). three types of nerve profiles were revealed: (1) 60-70% of the profiles are adrenergic; (2) less than 5% of the profiles appear to be cholinergic; (3) up to 40% of the profiles are distinguished by the presence of a characteristically high proportion of electron-opaque, chromaffin-negat ...197990550
glycosomes (protein-glycogen complex) in the canine heart. ultrastructure, histochemistry and changes induced by acidic treatment.cardiac conducting fibers were selected from two dogs defined as a and b. the specimens differed in the reaction of their electron dense granules, commonly referred to as glycogen, to the treatment en bloc with uranyl acetate. material was fixed in glutaraldehyde and oso4. blocks were processed either conventionally or immersed in uranyl acetate before dehydration. sections were examined unsatined, stained with u and/or pb or with a histochemical technique (pa-tsc-sp) specific for glycogen. elec ...197993343
substance p infusion into substantia nigra of the rat: behavioural analysis and involvement of striatal dopamine.the purpose of the present study was to characterize the nature of the behavioural response to substance p (sp) infusion into the substantia nigra and to evaluate this response in 6-hydroxydopamine (6-ohda) caudate lesioned rats. the effects of sp infusions (3 microgram in 1 microliter bilaterally) were assessed in an open field. two groups of rats were used: one with 6-ohda lesions in the caudate nucleus, and one with sham lesions. in sham rats, the first infusion produced a strong increase in ...197993550
in vivo release of substance p in the cat substantia nigra.push-pull cannuale were implanted into the substantia nigra (sn) and the caudate nucleus (cn) of the cat to study the in vivo release of substance p (sp), using a radioimmunoassay and high pressure liquid chromatography (hplc) analysis. the spontaneous release of sp could be detected in the sn and the cn. potassium (50 mm) locally applied stimulated sp release in both structures. furthermore an important evoked release of sp was observed in the sn during depolarization of striato-nigral sp fiber ...197993729
synthesis of substance p analogs and agonistic and antagonistic activities.fourteen analogs of substances p (sp), six previously synthesized by a solution method and eight newly synthesized by a solid-phase technique, have been tested for agonistic and antagonistic activities utilizing the isolated guinea pig ileum. the primary objective is to achieve effective inhibitors of sp. these analogs had agonistic activities ranging from negligible to that equivalent to sp. six of the fourteen analogs had some degree of antagonistic activity [d-leu8, d-phe9]-sp is an analog wh ...197994202
occurrence and distribution of rhizobiophages in indian soils.soil samples from 30 fields in neighbouring districts of varanasi were screened for rhizobiophages on 60 rhizobium strains. plaques were observed on five strains: p1, p5, su391 (r. leguminosarum), cb756 and 32h1 (rhizobium sp.). rhizobiophages infective on one or more of the five strains were present in all fields. there seems to be no correlation between presence of phage and the standing crop or the ph (7.1-8.2) of the soil. eight distinct rhizobiophages have been isolated and characterized fo ...197994751
effect of neurotensin, substance p and morphine sulfate on the secretion of prolactin and growth hormone in the rat.neurotensin (nt), substance p (sp) and morphine sulfate (ms) elevate plasma prolactin and growth hormone levels in both normal or estrogen-progesterone pretreated male rats. by contrast, steroid priming is required for trf to exhibit prl-releasing activity. naloxone, an opiate receptor blocker, reverses the stimulatory effect of ms only. diphenhydramine, a histamine antagonist, inhibits the response to nt, sp and ms without affecting the response to trf. these results suggest the involvement of ...197795701
pyroglutamyl-n3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone) in the rat hypothalamus.pyroglutamyl-n3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone, methyl-trh) is a potent synthetic analogue of trh. n3im-methyl-histidine is present in mammalian brain and it has been suggested that methyl-trh is a physiological releasing hormone normally present in the hypothalamus. a non-gradient cation-exchange chromatography system that uses sp-sephadex c-25 and completely resolves methyl-trh and trh has been developed. because methyl-trh cross-reacts in the immunoassay ...197896203
purification and immunochemical quantitation of human pancreatic secretory trypsin inhibitor.human pancreatic secretory trypsin inhibitor has been purified from pancreatic juice obtained on connection with surgery in the pancreas. the purification was accomplished by gel filtration on sephadex g-75 and ion-exchange chromatography on sp-sephadex c-50 giving 49% yield. five forms of the inhibitor were demonstrated by combined chromatographic and electrophoretic methods. on gel filtration the inhibitor was shown to have the same molecular weight as the kunitz inhibitor, i.e. about 6500. in ...197896521
serologic studies of the maize stalk rot pathogen erwinia carotovora f. sp. zeae.with the help of serological techniques, namely microprecipitin, agglutination, and gel diffusion, sixteen isolates of maize stalk rot pathogen were proved to be identical. serological techniques have thus been utilized as an additional tool for identifying the pathogen erwinia carotovora f. sp. zea. it was also established that the pathogen does not perpetuate in the seed, either externally of internally. it could, however, be found through serological tests that infected ears carried the patho ...197896628
purification and partial characterization of three extracellular cellulases from cellulomonas sp.three extracellular cellulases have been purified from cultures of cellulomonas. one was found in solution in the cell-free supernatant and two others were found to be bound to the cellulose added as a carbon source. the free enzyme and one of the cellulose-bound enzymes bind to sephadex. the two cellulose-bound enzymes are glycosylated. the three enzymes behave as endocellulases towards soluble carboxymethyl-cellulose and have little activity on cellulose powder.197898328
bovine cryptosporidiosis: a transmission and scanning electron microscopic study of some stages in the life cycle and of the host-parasite relationship.transmission and scanning electron microscopy of the ileal mucosae from 12 calves infected with cryptosporidium sp. showed cryptosporidia free in the lumen and attached to epithelium. the attached parasites were interpreted to be extracellular and adherent to the microvillous border of epithelial cells. stages of the organism included crescentic-free merozoites, trophozoites, schizonts, gametes and oocysts. attached parasites were detected chiefly at villous tips and all stages were present on a ...197898896
diurnal variation of serum androgen and estradiol-17beta in the adult male green monkey (cercopithecus sp). 197899184
a zoogloea sp. associated with blooms of anabaena flos-aquae.bacteria were found attached to the heterocysts of aphanizomenon flos-aquae and embedded within the mucilage of both anabaena flos-aquae and microcystis aeruginosa in freshwater plankton. electron microscopy of thin sections preceding the peak of an anabaena flos-aquae bloom showed that the density of bacterial cells was 7.4 x 10(5) cells/ml in the planktonic macroenvironment and 2.6 x 10(11) cells/ml within the microenvironment of cyanobacterial mucilage. the bacteria occurred in aggregates and ...197899218
comparison of spontaneous, uv-induced, and nitrosoguanidine-induced mutability to drug resistance in myxobacteria.the uv survival curves of different strains of myxobacteria exhibited shoulders; in the case of polyangium luteum, an unusual double shoulder appeared. repair inhibitors like acriflavine, caffeine, and coumarin reduced the survival of uv-irradiated cells if the drugs were incorporated in the post-irradiation plating medium. the shoulders were reduced, but the final inactivation slopes were not affected by the repair inhibitors. those strains that were resistant to uv were also more resistant to ...197899434
dark hexose metabolism by photoautotrophically and heterotrophically grown cells of the blue-green alga (cyanobacterium) nostoc sp. strain mac.photoautotrophically grown cells of the blue-green alga (cyanobacterium) nostoc sp. strain mac assimilated and oxidized both glucose and fructose in the dark at different rates. the rate of fructose metabolism in these cells could be stimulated by casein hydrolysate, the effect being most pronounced at low sugar concentrations. this stimulation was not seen in cells grown heterotrophically in the dark, suggesting that it is a transitory phenomenon which disappears during the autotrophy-heterotro ...197899438
[allergization of the body by neisseria meningitidis. i. the problem of the occurrence of n. meningitidis in cases of bronchial asthma of bacterial etiology (author's transl)].the object of the authors work was the examination of nasopharyngeal swabs on the prevalence of neisseria meningitidis. a group of 222 patients suffering from asthma bronchiale of bacteriological etiology was under observation in order to be able to prepare an autovaccine. nasopharyngeal swabs were inoculated on special nutrient media. neisseria meningitidis was found in 22%, mainly from nasopharyngeal swabs, together with haemophilus sp. and streptococcus pneumoniae. in 59% of examined cases th ...197899912
physiological and morphological observations on thiovulum sp.cell suspensions of thiovulum sp., collected from enrichment cultures, were grown, maintained, and harvested for periods up to 7 months. in open-flow cultures run with aerated seawater, a continuous supply of hydrogen sulfide was provided by diffusion through a semipermeable membrane from either a live culture of desulfovibrio esturaii, neutralized sodium sulfide, or a n2-h2s gas mixture. attempts to grow thiovulum in pure culture failed despite variation in concentrations of dissolved oxygen an ...1978101531
cyanobacterial phycobilisomes. characterization of the phycobilisomes of synechococcus sp. 6301.a procedure is described for the preparation of stable phycobilisomes from the unicellular cyanobacterium synechococcus sp. 6301 (also known as anacystis nidulans). excitation of the phycocyanin in these particles at 580 nm leads to maximum fluorescence emission, from allophycocyanin and allophycocyanin b, at 673 nm. electron microscopy shows that the phycobilisomes are clusters of rods. the rods are made up of stacks of discs which exhibit the dimensions of short stacks made up primarily of phy ...1978101538
[studies on moraxella. i. a new selective medium for moraxella sp. (author's transl)]. 1977101603
[sulfobacillus, a new genus of thermophilic sporulating bacteria].an aerobic facultative thermophilic bacterium was isolated from the ore of the nikolaev copper-zinc-pyrite deposit in the eastern kazakhstan. the bacterium is similar to thiobacillus ferooxidans in the ability to use various energy subsrates (fe2+, sulphide minerals and so) and in the acidophilic properties, but differs from it in the thermophilic nature, the structure of a cell wall, the ability to produce spores, and a lower gc content in dna. consequently, the organism has been identified as ...1978101742
quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method.quantitative bactericidal activities of beta-lactam antibiotics were determined by the agar plate method. broth cultures, of which the colony-forming units were counted before the study, were inoculated on antibiotic-containing agar plates, utilizing a 10(-3), 10(-2), or 10(-1) dilution or undiluted culture plated with each 0.001-ml calibrated loop. these plates were incubated at 37 degrees c overnight, and the minimal drug concentration at which no bacterial growth was observed on the plates wa ...1978102246
[epidemiology of leishmaniasis in french guiana in relation to the existence of a new species of echimyid rodent, proechimys cuvieri sp. n].the rediscovery and reexamination of the skull of the type of proechimys guyannensis (e. geoffroy; 1803) allows us to give more details about this species. it appears that a new sympatric species, p. cuvieri, has to be distinguished, which could have great implications for the leishmaniosis epidemiology in french guiana.1978102437
wenyonella baghdadensis sp. n. from the bandicoot rat nesokia indica in the baghdad area.oocysts of wenyonella baghdadensis sp. n. were found in the feces of 2 of 12 bandicoot rats nesokia indica gray & harwicke from the baghdad area. sporulated oocysts were subspherical to broadly ellipsoidal, 25.4 (18-22) x 20.8 (15-28) micrometer, with a 2-layered wall, the outer wall being mammillated, without micropyle, micropylar cap, residuum, or polar granule. sporozoites were ovoid, 11.8 (9-15) x 8.4 (6-10) micrometer, with stieda body and residuum. two sporozoites in each sporocyst were el ...1978102783
ultrastruct of red-sore lesions on largemouth bass (micropterus salmoides): associattion of the ciliate epistylis sp. and the bacterium aeromonas hydrophila.epizootic outbreaks of red-sore disease in several reservoirs in the southeastern united states have been reported to cause heavy mortality among several species of fish having sport and commercial value. the etiologic agent is said to be the peritrich ciliate epistylis sp.; secondary infection by the gram-negative bacterium aeromonas hydrophila produces hemorrhagic septicemia which results in death. however, in recent studies on the largemouth bass micropterus salmoides, epistylis sp. could be ...1978102785
the incidence of theilerial parasites in east african buffalo (syncerus caffer).245 buffalo from 13 areas of east africa were examined for theilerial infections. the vast majority of buffalo (97.1%) examined had piroplasms in their erythrocytes. theileria lawrencei was isolated from the buffalo by tick feeding and cell culture and was found to be common in most of these buffalo populations. also over 50% of the buffalo had indirect fluorescent antibody (ifa) titres to t. lawrencei. t. mutans was only isolated from 3 buffalo populations but is probably common. haematoxenus s ...1978103262
mandibular biomechanics and temporomandibular joint function in primates.there is disagreement as to whether the mandibular condyles are stress-bearing or stress-free during mastication. in support of alternative models, analogies have been drawn with class iii levers, links, and couple systems. physiological data are reviewed which indicate that maximum masticatory forces are generated when maxillary and mandibular teeth are in contact, and that this phase lasts for over 100 msec during many chewing strokes. during this period, the mandible can be modeled as a beam ...1978103437
human alpha-1-antichymotrypsin: purification and properties.human alpha-1-antichymotrypsin has been purified to homogeneity by the following sequential steps--(a) ammonium sulfate fractionation; (b) chromatography on cibacron blue sepharose at ph 7.0; and (c) chromatography on sp-sephadex c-50 at ph 5.5. the inhibitor has a molecular weight near 68,000 and contains approximately 26% carbohydrate alpha-1-antichymotrypsin has an amino-terminal arginine and a carboxy-terminal glycine. it also has some homology with alpha-1-pi based on amino-terminal sequenc ...1978103576
[efficacy, tolerance, and pharmacokinetics of sisomicin in urinary tract infections (author's transl)].since 1973 30 patients with urinary tract infections (uti) or pyelonephritis have been treated with sisomicin, a new aminoglycoside, in a daily dose of 2 mg/kg for a period of seven to nine days. from a clinical point of view the result of treatment was good. complete resolution was achieved in 17 patients, improvement in nine, and there was no effect in four patients. thirty-five causative organisms (escherichia coli = 23, proteus sp. = 7, klebsiella sp. = 3, pseudomonas aeruginosa = 1, citroba ...1978103844
effects of some inorganic elements on nitrogen-fixation in blue-green algae and some ecological aspects of pollution.nitrogen-fixation by two species of nostoc, one of them a lichen phycobiont, was generally stimulated by low concentrations of arsenic, cadmium, nickel, lead, palladium, and zinc. higher concentrations (0.025 to 0.125 ppm) of arsenic, nickel, and palladium were also stimulatory; however, higher concentrations of cadmium, lead, and zinc tended to inhibit fixation. with the exception of palladium and zinc at low concentrations these six tested elements tended to inhibit nitrogen-fixation in chloro ...1978104450
studies on acute and late stages of experimental central retinal artery occlusion in the cynomolgus monkey. ii. influence on the cyclic changes in the amplitude of the c-wave of the erg and in the standing potential of the eye.the slow (2-3/h) oscillations of the c-wave amplitude and of the standing potential of the eye (sp) were studied in the cynomolgus monkey during the acute and late stages after experimentally induced (laser photocoagulation) occlusion of the central retinal artery (ocra). whereas the healthy control eyes showed large cyclic variations in both the c-wave amplitude and the sp, no oscillations in the c-wave amplitude were observed in the ocra eyes at any stage, and the sp oscillations were barely d ...1978104543
the femur in early human evolution.uni- and multivariate analyses of 5 fossil and 215 extant hominoid femora show that two morphological patterns of hominid femora existed about two million years ago. femora classified as homo sp. indet. (knmer 1472 and 1481) are more like homo sapiens although not identical. those classified as australopithecus robustus (sk 82 and 97) and a. boisei (knm-er 1503) are similar to one another but uniquely different from any living hominoid. the strong mophological constrasts imply biomechanical and ...1978104627
cc-1065 (nsc-298223), a new antitumor antibiotic. production, in vitro biological activity, microbiological assays and taxonomy of the producing microorganism.a new antitumor antibiotic is produced in fermentation liquors of streptomyces zelensis sp.n. the antibiotic is biologically active at extremely low concentrations. at 40 pg/ml, it inhibited 90% of the growth of l1210 cells in culture in tube dilution assays. the minimal inhibitory concentrations against gram-positive bacteria is between 1 approximately 10 ng/ml, while these values for gram-negative bacteria and fungi are mostly under 1 microgram/ml. a microbiological assay with bacillus subtili ...1978104946
homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria.the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ...1979104972
serious infections from bacillus sp.serious infections caused by organisms of the genus bacillus developed in seven patients. five drug abusers had either endocarditis or osteomyelitis, one leukemic patient had necrotizing fasciitis, and one patient had a ventriculoatrial shunt infection with recurrent bacteremia. all patients recovered. experience with these cases reemphasizes the importance of not dismissing bacillus organisms as culture contaminants, especially when isolated from blood, body fluids, or closed-space infections.1979105158
sarcocystis sp from the goat in india. 1978105455
azo reduction of trypan blue to a known carcinogen by a cell-free extract of a human intestinal anaerobe.the azo reductase activity of a cell-free extract of fusobacterium sp. 2 is characterized using trypan blue as a substrate. either chemical reduction of this dye with sodium hydrosulfite or reduction by the cell-free extract produces a mutagenic product, o-tolidine. the o-tolidine is mutagenic in the ames salmonella/mammalian-microsome mutagenicity test when activated by a rat liver s9 preparation.1978106267
[monitoring the helmintological situation in a large-scale reproduction of pigs].in 1976 to 1978, the occurrence of helminths in a large reproduction herd of pigs was studied in different age categories of the animals kept. six helminth species were found in 30.7% of the sows: ascaris suum (6.6%), oesophagostomum dentatum (24.0%), hyostrongylus rubidus (3.3%), trichocephalus suis (1.3%), strongyloides ransomi (0.7%), metastrongylus sp. (0.3%). four- to seven-month-old auction gilts had only the eggs of a. suum (4% of all cases). no helminith eggs were found in fattened pigle ...1979106504
a novel tetrahymena sp. with unusual membrane lipid composition, with special reference to iso fatty acyl chains.a novel tetrahymena sp. is similar in appearance to four classical strains (wh-14, gl, w, nt-1) of t. pyriformis except for a larger width. the phospholipid and acyl chain compositions of membranes of this novel species are significantly different from those of other strains. the tetrahymena sp. has a much higher content of ethanolamine phosphoglyceride and a lower level of glyceroaminoethyl phosphonate. major fatty acids of this strain are iso 15:0 and iso 17:1, which are, if present, usually m ...1978107352
localization of hypophysiotropic peptides and other biologically active peptides within the brain.immunohistochemical analysis of hypophysiotropic and other neuropeptides reveals unique and striking neural perikarya, axons, and terminals containing specific peptide immunoreactivity. the hypophysiotropic peptides are most highly concentrated in nerve terminals in the external layer of the median eminence. from this site they may be released and carried via the portal circulation to the adenohypophysis. the occurrence of hypophysiotropic peptides in other areas of the nervous system suggests t ...1979107852
[new species of microsporidians (microsporidia, nosematidae) from horsefly larvae in the ukraine].a new species of microsporidians, stempellia lairdi sp. n., was found in larvae of tabanus autumnalis l. from ukraine. it affects the muscular tissue and haemolymph of horse fly larvae. spores of the species are oval, egg-shaped, 1.8--7.6 x 1.2--3.8 mu in size. the species is pathogenic and causes the mortality of infected larvae.1979108652
[new species of parasitic infusoria of the family urceolariidae].three new species of ciliata of the family urceolariidae were found as follows: paratrichodina voikarensis sp. n. on the gills of coregonus nasus and c. peled; p. uralensis sp. n. on the gills of the sterlet and trichodina percae sp. n. in the nasal cavities of the perch. figures and descriptions of the new species are given.1979108655
decomposition of ddt and its analogs by soil microflora.a search was made for active cultures that break down ddt. more than 600 microorganisms were isolated from soils treated for a long time with ddt and from accumulating cultures with ddt or its analogs as the carbon source and with ddt or its analogs and supplementary carbon sources. cultures capable of degrading ddt under conditions of cometabolism were found among them. it was shown that the dechlorination of the trichloromethyl group of ddt was accomplished by a large number of microorganisms ...1978109130
hematozoa from southern african vultures, with a description of haemoproteus janovyi sp. n.this study represents the first major survey of avian hematozoa from southern africa and the only one dealing with blood parasites of vultures. blood smears from 506 rhodesian, botswanan and south african vultures (hooded, white-headed, lappetfaced, cape griffon and whitebacked vultures) were examined for hematozoa. haemoproteus janovyi sp. n. was observed in 35.2% of the vultures, leucocytozoon toddi in 0.8%, plasmodium fallax in 0.6%, atoxoplasma sp. in 1.4% and microfilariae in 0.2%. hematozo ...1979109605
rocky mountain spotted fever in connecticut: human cases, spotted-fever group rickettsiae in ticks, and antibodies in mammals.three parameters were used in 1976 and 1977 to assess the status of rocky mountain spotted fever (rmsf) in connecticut--compilation and review of clinical data on suspected human cases for the 13-year period 1965--1977, examination of tick tissues for spotted fever-group rickettsiae by the hemolymph test and direct immunofluorescence, and analyses of mammalian sera for antibodies against rickettsia rickettsii. there were six presumptive rmsf cases which probably originated in connecticut. four o ...1979111543
light and electron microstructure of a sarcocystis sp. from the malaysian long-tailed monkey, macaca fascicularis.the ultrastructure of the cyst wall and zoites of a species of sarcocystis from the skeletal muscles of a naturally-infected malaysian long-tailed monkey, macaca fascicularis, is described in detail. the wavy, electron-dense primary cyst wall is thin (55 nm) and invaginated. cytophaneres are absent. the ground substance contains electron-dense granules and bundles of parallel, fibrillar elements in some areas. thin trabeculae are present. the zoites measure 1.2 x 4.7 microns and have an interior ...1979111569
dihydrofolate reductase from neisseria sp.members of the genus neisseria are relatively nonsusceptible to trimethoprim, an inhibitor of dihydrofolate reductase. for example, the minimal inhibitory concentration (mic) of trimethoprim for n. gonorrhoeae ranges from 2 to 70 mug/ml, whereas the mic for escherichia coli is 0.2 mug/ml or less. in an effort to understand this difference, dihydrofolate reductase was partially purified from five neisseria species and compared with the enzyme from e. coli. n. gonorrhoeae dihydrofolate reductase w ...1979111611
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