| wolbachia screening in spiders and assessment of horizontal transmission between predator and prey. | recent studies have revealed that the prevalence of wolbachia in arthropods is attributable not only to its vertical transmission, but also to its horizontal transfer. in order to assess the horizontal transmission of wolbachia between predator and prey, arthropods belonging to 11 spider families and six insect families were collected in the same field of rice. the distribution of wolbachia in these arthropods was detected by diagnostic pcr amplification of the wsp (wolbachia outer surface prote ... | 2011 | 21584395 |
| genetic dissection of the resistance to rice stripe virus present in the indica rice cultivar 'ir24' | rice stripe disease, caused by rice stripe virus (rsv) and transmitted by the small brown planthopper (laodelphax striatellus fallen), is one of the most serious viral diseases of rice in temperate east asian production regions. prior quantitative trait loci (qtl) mapping has established that oryza sativa l. subsp. indica 'ir24' carries positive alleles at the three loci qstv3, qstv7, and qstv11-i. here, we report an advanced backcross analysis based on three selected chromosome segment substitu ... | 2011 | 21793697 |
| planthopper "adaptation" to resistant rice varieties: changes in amino acid composition over time. | the brown planthopper, nilaparvata lugens, shows considerable geographic and temporal variability in its response to varieties of cultivated rice. n. lugens has repeatedly "adapted" to resistant rice varieties; however, the physiological changes underlying planthopper adaptation are poorly understood. endosymbionts within planthoppers, such as yeast-like endosymbionts (yls) could play a role as they produce essential amino acids for planthoppers. we used a full factorial study to determine how n ... | 2011 | 21782824 |
| multi-generation effects of bt rice on anagrus nilaparvatae, a parasitoid of the nontarget pest nilapavarta lugens. | little is known about the potential cumulative long-term effects of transgenic crops on nontarget organisms. in the present laboratory study, the potential cumulative effects of transgenic bacillus thuringiensis (bt) rice on parasitoids in successive generations were observed for an egg parasitoid, anagrus nilaparvatae parasitizing eggs of nilaparvata lugens (stål) (hemiptera: delphacidae) feeding on bt rice. enzyme-linked immunosorbent assay test confirmed that cry1ab insecticidal protein coul ... | 2010 | 22182572 |
| design, synthesis and insecticidal activities of novel pyrazole amides containing hydrazone substructures. | background: pyrazole and hydrazone derivatives possess good insecticidal activities; their substructural units are widely used in pesticide design. in an effort to discover new molecules with good insecticidal activities, a series of pyrazole amide derivatives containing hydrazone substructures were synthesised and bioassayed. results: bioassays demonstrated that some of the title compounds exhibited notable control of plutella xylostella (linnaeus), helicoverpa armigera (hübner), culex pipiens ... | 2011 | 22190278 |
| five proteins of laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector. | rice stripe virus (rsv) is the type member of the genus tenuivirus, which relies on the small brown planthopper (laodelphax striatellus fallén) for its transmission in a persistent, circulative-propagative manner. to be transmitted, virus must cross the midgut and salivary glands epithelial barriers in a transcytosis mechanism where vector receptors interact with virions, and as propagative virus, rsv need utilize host components to complete viral propagation in vector cells. at present, these m ... | 2011 | 22028913 |
| population dynamics of noncytoplasmic incompatibility-inducing wolbachia in nilaparvata lugens and its effects on host adult life span and female fitness. | wolbachia are bacteria that live intracellularly in a wide variety of arthropods. they are maternally inherited and can affect both reproduction and fitness of its host. when infected males mate with uninfected females or females infected by a different wolbachia strain, there is often a failure of karyogamy, which is usually attributed to cytoplasmic incompatibility (ci). we measured the strength of ci induced by wolbachia and the fitness effects in three chinese populations of the brown plant ... | 2010 | 22182545 |
| Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control. | Yeasts associate with numerous insects, and they can assist the metabolic processes within their hosts. Two distinct yeasts were identified by PCR within the planthopper Perkinsiella saccharicida, the vector of Fiji disease virus to sugarcane. The utility of both microbes for potential paratransgenic approaches to control Fiji leaf gall (FLG) was assessed. Phylogenetic analysis showed one of the microbes is related to yeast-like symbionts from the planthoppers: Laodelphax striatellus, Nilaparvat ... | 2011 | 21850475 |
| suppression of ns3 and mp is important for the stable inheritance of rnai-mediated rice stripe virus (rsv) resistance obtained by targeting the fully complementary rsv-cp gene. | rice stripe virus (rsv) is a viral disease that seriously impacts rice production in east asia, most notably in korea, china, and japan. highly rsv-resistant transgenic japonica rice plants were generated using a dsrnai construct designed to silence the entire sequence region of the rsv-cp gene. transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (sbph), and their resistance was evaluated using elisa and an infection rate assay. a correlatio ... | 2011 | 22134721 |
| identification and function analysis of enolase gene nleno1 from nilaparvata lugens (stål) (hemiptera:delphacidae). | the enolase [ec 4.2.1.11] is an essential enzyme in the glycolytic pathway catalyzing the conversion of 2-phosphoglycerate (2-pge) to phosphoenolpyruvate (pep). in this study, a full-length cdna encoding α-enolase was cloned from rice brown planthopper (nilaparvata lugens) and is provisionally designated as nleno1. the cdna sequence of nleno1 was 1,851 bp with an open reading frame (orf) of 1,305 bp and encoding 434 amino acids. the deduced protein shares high identity of 80-87% with eno1-like p ... | 2015 | 26056319 |
| identification of transcription factors potential related to brown planthopper resistance in rice via microarray expression profiling. | brown planthopper (bph), nilaparvata lugens stål, is one of the most destructive insect pests of rice. the molecular responses of plants to sucking insects resemble responses to pathogen infection. however, the molecular mechanism of bph-resistance in rice remains unclear. transcription factors (tf) are up-stream regulators of various genes that bind to specific dna sequences, thereby controlling the transcription from dna to mrna. they are key regulators for transcriptional expression in biolog ... | 2012 | 23228240 |
| synthesis, insecticidal, and antibacterial activities of novel neonicotinoid analogs with dihydropyridine. | nilaparvata lugens, a major pest in rice-growing areas, is extremely difficult to manage. neonicotinoids have increasingly been used in crop protection and animal health care against n. lugens. to discover new bioactive molecules and pesticides, we combined the active structure of cyanoacrylates, aromatic aldehydes, and substituted pyridyl (thiazolyl) methyl-2-substituted-methylidene-imidazolidine derivatives for the design and synthesis of a series of novel neonicotinoid analogs with dihydropyr ... | 2013 | 23621983 |
| comparing gene expression profiles between bt and non-bt rice in response to brown planthopper infestation. | bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. we previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (bph) fed on bt rice line kmd2, although bt insecticidal protein cry1ab could rarely concentrate in this non-target rice pest. in the present study, we performed microarray analysis in an effort to detect bt-independent variation, which might render bt rice more defensive and/or less n ... | 2015 | 26734057 |
| silencing coi1 in rice increases susceptibility to chewing insects and impairs inducible defense. | the jasmonic acid (ja) pathway plays a key role in plant defense responses against herbivorous insects. coronatine insensitive1 (coi1) is an f-box protein essential for all jasmonate responses. however, the precise defense function of coi1 in monocotyledonous plants, especially in rice (oryza sativa l.) is largely unknown. we silenced oscoi1 in rice plants via rna interference (rnai) to determine the role of oscoi1 in rice defense against rice leaf folder (lf) cnaphalocrocis medinalis, a chewing ... | 2012 | 22558386 |
| map-based cloning and characterization of the bph18 gene from wild rice conferring resistance to brown planthopper (bph) insect pest. | brown planthopper (bph) is a phloem sap-sucking insect pest of rice which causes severe yield loss. we cloned the bph18 gene from the bph-resistant introgression line derived from the wild rice species oryza australiensis. map-based cloning and complementation test revealed that the bph18 encodes cc-nbs-nbs-lrr protein. bph18 has two nbs domains, unlike the typical nbs-lrr proteins. the bph18 promoter::gus transgenic plants exhibited strong gus expression in the vascular bundles of the leaf shea ... | 2016 | 27682162 |
| temporal interactions of plant - insect - predator after infection of bacterial pathogen on rice plants. | pathogenic infection on plants may affect interactions of host-plants with their herbivores, as well as the herbivores with their predators. in this study, the effects of infection by pathogenic bacterium xanthomonas oryzae pv. oryzae (xoo), which causes a vascular disease in rice, on rice plants and consequent interactions with a rice herbivore, brown rice planthopper (bph) nilaparvata lugens, and its major predator, cyrtorhinus lividipennis, were investigated. the results showed that the rice ... | 2016 | 27185548 |
| proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein. | numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ... | 2015 | 26091699 |
| pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety. | pyramiding multiple genes into a desirable genetic background can take years to accomplish. in this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. pink3, an aromatic and potentially high-yielding rice variety-although one that is intolerant to flash flooding (sub) and susceptible to bacterial leaf blight (bb), leaf-neck blast (bl) and the brown planthopper (bph)-was used as a genetic basis for significant improvements through gene pyramiding. | 2015 | 25844112 |
| the transcription factor oswrky45 negatively modulates the resistance of rice to the brown planthopper nilaparvata lugens. | wrky transcription factors play a central role not only in plant growth and development but also in plant stress responses. however, the role of wrky transcription factors in herbivore-induced plant defenses and their underlying mechanisms, especially in rice, remains largely unclear. here, we cloned a rice wrky gene oswrky45, whose expression was induced by mechanical wounding, by infestation of the brown planthopper (bph, nilaparvata lugens) and by treatment with jasmonic acid (ja) or salicyli ... | 2016 | 27258255 |
| bph32, a novel gene encoding an unknown scr domain-containing protein, confers resistance against the brown planthopper in rice. | an urgent need exists to identify more brown planthopper (nilaparvata lugens stål, bph) resistance genes, which will allow the development of rice varieties with resistance to bph to counteract the increased incidence of this pest species. here, using bioinformatics and dna sequencing approaches, we identified a novel bph resistance gene, loc_os06g03240 (msu locus id), from the rice variety ptb33 in the interval between the markers rm19291 and rm8072 on the short arm of chromosome 6, where a gen ... | 2016 | 27876888 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos. | plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ... | 2015 | 25601039 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| genomic insights into the glutathione s-transferase gene family of two rice planthoppers, nilaparvata lugens (stål) and sogatella furcifera (horváth) (hemiptera: delphacidae). | glutathione s-transferase (gst) genes control crucial traits for the metabolism of various toxins encountered by insects in host plants and the wider environment, including insecticides. the planthoppers nilaparvata lugens and sogatella furcifera are serious specialist pests of rice throughout eastern asia. their capacity to rapidly adapt to resistant rice varieties and to develop resistance to various insecticides has led to severe outbreaks over the last decade. | 2013 | 23457591 |
| diapause induction and termination in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | the small brown planthopper, laodelphax striatellus (fallén) enters the photoperiodic induction of diapause as 3rd or 4th instar nymphs. the photoperiodic response curves in this planthopper showed a typical long-day response type with a critical daylength of approximately 11 h at 25 °c, 12 h at 22 and 20 °c and 12.5 h at 18 °c, and diapause induction was almost abrogated at 28 °c. the third stage was the most sensitive stage to photoperiod. the photoperiodic response curve at 20 °c showed a gra ... | 2014 | 25188306 |
| molecular cloning and characterization of an acetylcholinesterase cdna in the brown planthopper, nilaparvata lugens. | a full cdna encoding an acetylcholinesterase (ache, ec 3.1.1.7) was cloned and characterized from the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae). the complete cdna (2467 bp) contains a 1938-bp open reading frame encoding 646 amino acid residues. the amino acid sequence of the ache deduced from the cdna consists of 30 residues for a putative signal peptide and 616 residues for the mature protein with a predicted molecular weight of 69,418. the three residues (ser242, glu3 ... | 0 | 20874389 |
| overexpression of multiple detoxification genes in deltamethrin resistant laodelphax striatellus (hemiptera: delphacidae) in china. | the small brown planthopper (sbph), laodelphax striatellus (fallén), is one of the major rice pests in asia and has developed resistance to multiple classes of insecticides. understanding resistance mechanisms is essential to the management of this pest. biochemical and molecular assays were performed in this study to systematically characterize deltamethrin resistance mechanisms with laboratory-selected resistant and susceptible strains of sbph. | 2013 | 24324548 |
| seminal fluid protein genes of the brown planthopper, nilaparvata lugens. | seminal fluid proteins (sfps) are produced mainly in the accessory gland of male insects and transferred to females during mating, in which they induce numerous physiological and post-mating behavioral changes. the brown plant hopper (bph), nilaparvata lugens, is an economically important hemipterous pest of rice. the behavior and physiology of the female of this species is significantly altered by mating. sfps in hemipteran species are still unclear. | 2016 | 27538518 |
| roles of nlaktip in the growth and eclosion of the rice brown planthopper, nilaparvata lugens stål, as revealed by rna interference. | akt-interacting protein (aktip) interacts with serine/threonine protein kinase b (pkb)/akt. aktip modulates akt's activity by enhancing the phosphorylation of the regulatory site and plays a crucial role in multiple biological processes. in this study, the full length cdna of nlaktip, a novel aktip gene in the brown planthopper (bph) nilaparvata lugens, was cloned. the reverse transcription quantitive pcr (rt-qpcr) results showed that the nlaktip gene was strongly expressed in gravid female adul ... | 2015 | 26402675 |
| characterization and comparative analysis of small rnas in three small rna libraries of the brown planthopper (nilaparvata lugens). | the brown planthopper (bph), nilaparvata lugens (stå;l), which belongs to homopteran, delphacidae, is one of the most serious and destructive pests of rice. feeding bph with homologous dsrna in vitro can lead to the death of bph, which gives a valuable clue to the prevention and control of this pest, however, we know little about its small rna world. | 2012 | 22412935 |
| fork head transcription factor is required for ovarian mature in the brown planthopper, nilaparvata lugens (stål). | the brown planthopper (bph), nilaparvata lugens, is the most devastating rice pest in many areas throughout asia. the reproductive system of female n. lugens consists of a pair of ovaries with 24-33 ovarioles per ovary in most individuals which determine its fecundity. the fork head (fox) is a transcriptional regulatory molecule, which regulates and controls many physiological processes in eukaryotes. the fox family has several subclasses and members, and several fox factors have been reported t ... | 2011 | 22208615 |
| the genome of the nucleopolyhedrosis-causing virus from tipula oleracea sheds new light on the nudiviridae family. | a large double-stranded dna (dsdna) virus that produces occlusion bodies, typical of baculoviruses, has been described to infect crane fly larvae of the genus tipula (diptera, tipulidae). because of a lack of genomic data, this virus has remained unclassified. electron microscopy of an archival virus isolated from tipula oleracea, t. oleracea nudivirus (tonv), showed irregularly shaped occlusion bodies measuring from 2 to 5 μm in length and 2 μm in middiameter, filled with rod-shape virions cont ... | 2014 | 25540386 |
| brown planthopper nudivirus dna integrated in its host genome. | the brown planthopper (bph), nilaparvata lugens (hemiptera:delphacidae), is one of the most destructive insect pests of rice crops in asia. nudivirus-like sequences were identified during the whole-genome sequencing of bph. pcr examination showed that the virus sequences were present in all of the 22 bph populations collected from east, southeast, and south asia. thirty-two of the 33 nudivirus core genes were identified, including 20 homologues of baculovirus core genes. in addition, several gen ... | 2014 | 24574410 |
| transcriptome analysis of the small brown planthopper, laodelphax striatellus carrying rice stripe virus. | rice stripe virus (rsv), the type member of the genus tenuivirus, transmits by the feeding behavior of small brown planthopper (sbph), laodelphax striatellus. to investigate the interactions between the virus and vector insect, total rna was extracted from rsv-viruliferous sbph (rvls) and non-viruliferous sbph (nvls) adults to construct expressed sequence tag databases for comparative transcriptome analysis. over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of ... | 0 | 25288960 |
| pathways of amino acid degradation in nilaparvata lugens (stål) with special reference to lysine-ketoglutarate reductase/saccharopine dehydrogenase (lkr/sdh). | nilaparvata lugens harbors yeast-like symbionts (ylss). in present paper, a genome-wide analysis found 115 genes from ni. lugens and 90 genes from ylss that were involved in the metabolic degradation of 20 proteinogenic amino acids. these 205 genes encoded for 77 enzymes. accordingly, the degradation pathways for the 20 amino acids were manually constructed. it is postulated that ni. lugens can independently degrade fourteen amino acids (threonine, alanine, glycine, serine, aspartate, asparagine ... | 2015 | 26000452 |
| synergistic and compensatory effects of two point mutations conferring target-site resistance to fipronil in the insect gaba receptor rdl. | insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs to insects. here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects that combine to produce high and persistent levels of resistance to fipronil, an insecticide targeting on γ-aminobytyric acid (gaba) receptors. in nilaparvata lugens, a major pest of rice crops in many parts of asia, we ... | 2016 | 27557781 |
| annotated ests from various tissues of the brown planthopper nilaparvata lugens: a genomic resource for studying agricultural pests. | the brown planthopper (bph), nilaparvata lugens (hemiptera, delphacidae), is a serious insect pests of rice plants. major means of bph control are application of agricultural chemicals and cultivation of bph resistant rice varieties. nevertheless, bph strains that are resistant to agricultural chemicals have developed, and bph strains have appeared that are virulent against the resistant rice varieties. expressed sequence tag (est) analysis and related applications are useful to elucidate the me ... | 2008 | 18315884 |
| functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using rna interference. | rna interference (rnai) is an effective gene-silencing tool, and double stranded rna (dsrna) is considered a powerful strategy for gene function studies in insects. in the present study, we aimed to investigate the function of trehalase (tre) genes (tre 1-1, tre 1-2, and tre-2) isolated from the brown planthopper nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsrna. the results showed that tre1 an ... | 2016 | 27328657 |
| tor pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in nilaparvata lugens (stål). | the "target of rapamycin" (tor) nutritional signaling pathway and juvenile hormone (jh) regulation of vitellogenesis has been known for a long time. however, the interplay between these two pathways regulating vitellogenin (vg) expression remains obscure. here, we first demonstrated the key role of amino acids (aas) in activation of vg synthesis and egg development in nilaparvata lugens using chemically defined artificial diets. aas induced the expression of tor and s6k (s6 kinase), whereas rnai ... | 2016 | 27043527 |
| a genome-wide identification and analysis of the basic helix-loop-helix transcription factors in brown planthopper, nilaparvata lugens. | the basic helix-loop-helix (bhlh) transcription factors in insects play essential roles in multiple developmental processes including neurogenesis, sterol metabolism, circadian rhythms, organogenesis and formation of olfactory sensory neurons. the identification and function analysis of bhlh family members of the most destructive insect pest of rice, nilaparvata lugens, may provide novel tools for pest management. here, a genome-wide survey for bhlh sequences identified 60 bhlh sequences (nlbhlh ... | 2016 | 27869716 |
| insectbase: a resource for insect genomes and transcriptomes. | the genomes and transcriptomes of hundreds of insects have been sequenced. however, insect community lacks an integrated, up-to-date collection of insect gene data. here, we introduce the first release of insectbase, available online at http://www.insect-genome.com. the database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 mirnas of 69 insects, 96,925 pirnas of drosophila melanogaster and chilo suppressalis, 2439 lncrna of n ... | 2015 | 26578584 |
| ran involved in the development and reproduction is a potential target for rna-interference-based pest management in nilaparvata lugens. | ran (rangtpase) in insects participates in the 20-hydroxyecdysone signal transduction pathway in which downstream genes, ftz-f1, krüppel-homolog 1 (kr-h1) and vitellogenin, are involved. a putative ran gene (nlran) was cloned from nilaparvata lugens, a destructive phloem-feeding pest of rice. nlran has the typical ran primary structure features that are conserved in insects. nlran showed higher mrna abundance immediately after molting and peaked in newly emerged female adults. among the examined ... | 2015 | 26554926 |
| rnai knockdown of acetyl-coa carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, nilaparvata lugens. | a major challenge in ecology lies in understanding the coexistence of intraguild species, well documented at the organismal level, but not at the molecular level. this study focused on the effects of the antibiotic, jinggangmycin (jgm), a fungicide widely used in asian rice agroecosystems, on reproduction of insects within the planthopper guild, including the brown planthopper (bph) nilaparvata lugens and the white-backed planthopper (wbph) sogatella furcifera, both serious resurgence rice pests ... | 2015 | 26482193 |
| rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant. | most plant viruses that seriously damage agricultural crops are transmitted by insects. however, the mechanisms enabling virus transmission by insect vectors are poorly understood. the brown planthopper (nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including rice ragged stunt virus (rrsv). in this study, we investigated the mechanisms of rrsv transmission from its insect vector to the rice p ... | 2015 | 26073458 |
| nutritional signaling regulates vitellogenin synthesis and egg development through juvenile hormone in nilaparvata lugens (stål). | insect female reproduction which comprises the synthesis of vitellogenein (vg) in the fat body and its incorporation into developing oocytes, needs a large amount of energy and food resources. our previous studies found that juvenile hormone (jh) regulates vitellogenesis in the brown planthopper, nilaparvata lugens. here, we report on the role of jh in nutrient-regulated vg synthesis and egg development. we first cloned the genes coding for juvenile hormone acid methyltransferase (jhamt) which i ... | 2016 | 26927076 |
| genomic insights into the serine protease gene family and expression profile analysis in the planthopper, nilaparvata lugens. | the brown planthopper (nilaparvata lugens) is one of the most destructive rice plant pests in asia. n. lugens causes extensive damage to rice by sucking rice phloem sap, which results in hopper burn (complete death of the rice plants). despite its importance, little is known about the digestion, development and defense mechanisms of this hemimetabolous insect pest. in this study, we aim to identify the serine protease (sp) and serine protease homolog (sph) genes, which form a large family in euk ... | 2014 | 24952583 |
| transcriptome analysis of fat bodies from two brown planthopper (nilaparvata lugens) populations with different virulence levels in rice. | the brown planthopper (bph), nilaparvata lugens (stål), one of the most serious rice insect pests in asia, can quickly overcome rice resistance by evolving new virulent populations. the insect fat body plays essential roles in the life cycles of insects and in plant-insect interactions. however, whether differences in fat body transcriptomes exist between insect populations with different virulence levels and whether the transcriptomic differences are related to insect virulence remain largely u ... | 2014 | 24533099 |
| the genome- and transcriptome-wide analysis of innate immunity in the brown planthopper, nilaparvata lugens. | the brown planthopper (nilaparvata lugens) is one of the most serious rice plant pests in asia. n. lugens causes extensive rice damage by sucking rice phloem sap, which results in stunted plant growth and the transmission of plant viruses. despite the importance of this insect pest, little is known about the immunological mechanisms occurring in this hemimetabolous insect species. | 2013 | 23497397 |
| cloning and characterization of a putative farnesoic acid o-methyltransferase gene from the brown planthopper, nilaparvata lugens. | juvenile hormone (jh) plays key roles in both metamorphosis and adult reproductive processes. farnesoic acid o-methyltransferase (famet) is thought to be an important enzyme in the jh biosynthetic pathway, catalyzing methylation of farnesoic acid (fa) to methyl farnesoate (mf). a full-length cdna (nlfamet) encoding a 299 amino acid putative famet was isolated from the brown planthopper, nilaparvata lugens (stal) (hemiptera: geometroidea), a major rice pest in many parts of asia. nlfamet showed h ... | 0 | 20874390 |
| genomes of the rice pest brown planthopper and its endosymbionts reveal complex complementary contributions for host adaptation. | the brown planthopper, nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. outbreaks of it have re-occurred approximately every three years in asia. it has also been used as a model system for ecological studies and for developing effective pest management. to better understand how a monophagous sap-sucking arthropod herbivore has adapted to its exclusive host selection and to provide in ... | 2014 | 25609551 |
| genome sizes of nine insect species determined by flow cytometry and k-mer analysis. | the flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five hemipterans, and two hymenopterans. among which, the coleopteran lissorhoptrus oryzophilus (kuschel) had the largest genome of 981 mb. the average genome size was 504 mb, suggesting that insects have a moderate-size genome. compared with the insects in other orders, hymenopterans had small genomes, which were averagely about ~200 mb. we found that the genome ... | 2016 | 27932995 |
| genome-wide identification of long noncoding rna genes and their potential association with fecundity and virulence in rice brown planthopper, nilaparvata lugens. | the functional repertoire of long noncoding rna (lncrna) has been characterized in several model organisms, demonstrating that lncrna plays important roles in fundamental biological processes. however, they remain largely unidentified in most species. understanding the characteristics and functions of lncrna in insects would be useful for insect resources utilization and sustainable pest control. | 2015 | 26437919 |
| transcriptome and comparative gene expression analysis of sogatella furcifera (horváth) in response to southern rice black-streaked dwarf virus. | the white backed planthopper (wbph), sogatella furcifera (horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. southern rice black-streaked dwarf virus (srbsdv), transmitted by wbph, has become a great threat to rice production in east asia. | 2012 | 22558400 |
| distinct expression profiles and different functions of odorant binding proteins in nilaparvata lugens stål. | odorant binding proteins (obps) play important roles in insect olfaction. the brown planthopper (bph), nilaparvata lugens stål (delphacidae, auchenorrhyncha, hemiptera) is one of the most important rice pests. its monophagy (only feeding on rice), wing form (long and short wing) variation, and annual long distance migration (seeking for rice plants of high nutrition) imply that the olfaction would play a central role in bph behavior. however, the olfaction related proteins have not been characte ... | 2011 | 22174925 |
| apterous a modulates wing size, bristle formation and patterning in nilaparvata lugens. | apterous a (apa), a member of the lim-homeobox gene family, plays a critical role in the development of wing. the achaete-scute complex (as-c) encodes basic helix-loop-helix (bhlh) transcription factors and functions in bristle development. in the present study, we cloned apa (nlapa) and an achaete-scute homologue (nlash) from n. lugens. levels of nlapa and nlash were higher in nymphs than adults, with particularly high expression in the thorax of nymphs. nlapa expressed more highly in nymphs of ... | 2015 | 25995006 |
| variation among conventional cultivars could be used as a criterion for environmental safety assessment of bt rice on nontarget arthropods. | the current difficulty facing risk evaluations of bacillus thuringiensis (bt) crops on nontarget arthropods (ntas) is the lack of criteria for determining what represents unacceptable risk. in this study, we investigated the biological parameters in the laboratory and field population abundance of nilaparvata lugens (hemiptera: delphacidae) on two bt rice lines and the non-bt parent, together with 14 other conventional rice cultivars. significant difference were found in nymphal duration and fec ... | 2017 | 28167821 |
| cry1ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider pardosa pseudoannulata. | the impact of bacillus thuringiensis (bt) toxin proteins on non-target predatory arthropods is not well understood at the cellular and molecular levels. here, we investigated the potential effects of cry1ab expressing rice on fecundity of the wolf spider, pardosa pseudoannulata, and some of the underlying molecular mechanisms. the results indicated that brown planthoppers (bphs) reared on cry1ab-expressing rice accumulated the cry toxin and that reproductive parameters (pre-oviposition period, p ... | 2016 | 27452121 |
| loop replacements with gut-binding peptides in cry1ab domain ii enhanced toxicity against the brown planthopper, nilaparvata lugens (stål). | bacillus thuringiensis (bt) cry toxins have been used widely in pest managements. however, cry toxins are not effective against sap-sucking insects (hemiptera), which limits the application of bt for pest management. in order to extend the insecticidal spectrum of bt toxins to the rice brown planthopper (bph), nilaparvata lugens, we modified cry1ab putative receptor binding domains with selected bph gut-binding peptides (gbps). three surface exposed loops in the domain ii of cry1ab were replaced ... | 2016 | 26830331 |
| transgenic cry1c or cry2a rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | transgenic rice producing the insecticidal protein from bacillus thuringiensis berliner (bt) is protected from damage by lepidopteran insect pests. however, one of the main concerns about bt rice is the potential impact on non-target herbivores. in the present study, the ecological impacts of two bt rice lines, t1c-19 expressing cry1c protein and t2a-1 expressing cry2a protein, on the non-target herbivore brown planthopper (bph), nilaparvata lugens (stål), were evaluated under laboratory and fie ... | 2015 | 25067834 |
| single and fused transgenic bacillus thuringiensis rice alter the species-specific responses of non-target planthoppers to elevated carbon dioxide and temperature. | the approval of transgenic bacillus thuringiensis (bt) rice by china was momentous for biotech crops, although it has yet to be approved for commercial production. non-target pest problems in rice paddies, such as the three ecologically similar species of planthoppers nilaparvata lugens, laodelphax striatellus and sogatella furcifera, could become increasingly serious under global climate change. fused (cry1ab/cry1ac) and single (cry1ab) transgenic bt rice were evaluated for effects on species-s ... | 2014 | 24136625 |
| proteolytic processing of bacillus thuringiensis toxin cry1ab in rice brown planthopper, nilaparvata lugens (stål). | to understand the low toxicity of cry toxins in planthoppers, proteolytic activation of cry1ab in nilaparvata lugens was studied. the proteolytic processing of cry1ab protoxin by n. lugens midgut proteases was similar to that by trypsin activated cry1ab. the cry1ab processed with n. lugens midgut proteases was highly insecticidal against plutella xylostella. however, cry1ab activated either by trypsin or the gut proteases of the brown planthopper showed low toxicity in n. lugens. binding analysi ... | 2013 | 24021715 |
| transgenic cry1ab rice does not impact ecological fitness and predation of a generalist spider. | the commercial release of rice genetically engineered to express a cry1ab protein from bacillus thuringiensis (bt) for control of lepidoptera in china is a subject of debate. one major point of the debate has focused on the ecological safety of bt rice on nontarget organisms, especially predators and parasitoids that help control populations of insect pests. | 2012 | 22511982 |
| selection of beauveria isolates pathogenic to adults of nilaparvata lugens. | the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), is a destructive invasive pest and has become one of the most economically-important rice pests in china. effective control measures are desperately needed. entomopathogenic fungi, such as beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: clavicipitaceae) and b. brongniartii (saccardo), have shown great potential for the management of some sucking pest species. in this study, to explore alternative strategies fo ... | 2014 | 25373179 |
| analysis of yeast-like symbiote diversity in the brown planthopper (bph), nilaparvata lugens stål, using a novel nested pcr-dgge protocol. | yeast-like symbiotes (yls) are endosymbionts that are intimately associated with the growth, development, reproduction of their host, the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). however, it is unclear how many species of yls are found within n. lugens, and how they are related to each other. traditional methods or simple amplification based on 18s rdna sequence does not reliably identify new species quickly and efficiently. therefore, a novel nested pcr-denaturing gradien ... | 2013 | 23549902 |
| biochemical mechanisms of imidacloprid resistance in nilaparvata lugens: over-expression of cytochrome p450 cyp6ay1. | imidacloprid is a key insecticide extensively used for control of nilaparvata lugens, and its resistance had been reported both in the laboratory selected strains and field populations. a target site mutation y151s in two nicotinic acetylcholine receptor subunits and enhanced oxidative detoxification have been identified in the laboratory resistant strain, contributing importantly to imidacloprid resistance in n. lugens. to date, however, imidacloprid resistance in field population is primarily ... | 2013 | 23994173 |
| permanent genetic resources added to molecular ecology resources database 1 december 2011-31 january 2012. | this article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: barteria fistulosa, bombus morio, galaxias platei, hematodinium perezi, macrocentrus cingulum brischke (a.k.a. m. abdominalis fab., m. grandii goidanich or m. gifuensis ashmead), micropogonias furnieri, nerita melanotragus, nilaparvata lugens stål, sciaenops ocellat ... | 2012 | 22448966 |
| infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems. | himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ... | 2015 | 26470259 |
| unusual characteristics of dicistrovirus-derived small rnas in the small brown planthopper, laodelphax striatellus. | in this study, sequences of small rna (srna) libraries derived from the insect vector laodelphax striatellus were assembled into contigs and used as queries for database searches. a large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi p virus (hipv). interestingly, hipv-derived srnas had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. hipv srnas had a strong bias towa ... | 2014 | 24323637 |
| identification of himetobi p virus in the small brown planthopper by deep sequencing and assembly of virus-derived small interfering rnas. | profiling and assembly of virus-derived small interfering rnas (sirnas) using next-generation sequencing technologies have been very useful for identification and diagnosis of a number of plant and invertebrate viruses. in this work, we have conducted high-throughput pyrosequencing and bioinformatic analysis of the small brown planthopper (sbph, laodelphax striatellus), and these analyses unexpectedly showed that the himetobi p virus (hipv) was present in our laboratory cultures. hipv was also f ... | 2014 | 24239755 |
| molecular characterization, expression analysis and rnai knock-down of elongation factor 1α and 1γ from nilaparvata lugens and its yeast-like symbiont. | in the present paper, four cdnas encoding the alpha and gamma subunits of elongation factor 1 (ef-1) were cloned and sequenced from nilaparvata lugens, named nlef-1α, nlef-1γ, and its yeast-like symbiont (yls), named ysef-1α and ysef-1γ, respectively. comparisons with sequences from other species indicated a greater conservation for ef-1α than for ef-1γ. nlef-1α has two identical copies. the deduced amino acid sequence homology of nlef-1α and nlef-1γ is 96 and 64%, respectively, compared with ho ... | 2016 | 27809951 |
| a floatable formulation and laboratory bioassay of pandora delphacis (entomophthoromycota: entomophthorales) for the control of rice pest nilaparvata lugens stål (hemiptera: delphacidae). | the brown planthopper (bph), nilaparvata lugens (staparvata luera: delphacidae), is a serious rice pest that easily develops resistance to chemical insecticides and resistant rice varieties. this study evaluated the infectivity of the bph fungal pathogen, pandora delphacis, and developed a novel formulation as an alternative means of bph control. | 2016 | 25641904 |
| virus altered rice attractiveness to planthoppers is mediated by volatiles and related to virus titre and expression of defence and volatile-biosynthesis genes. | viruses may induce changes in plant hosts and vectors to enhance their transmission. the white-backed planthopper (wbph) and brown planthopper (bph) are vectors of southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv), respectively, which cause serious rice diseases. we herein describe the effects of srbsdv and rrsv infections on host-selection behaviour of vector and non-vector planthoppers at different disease stages. the y-tube olfactometer choice and free-choic ... | 2016 | 27924841 |
| rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| infection of rice plants by rice black streaked dwarf virus improves an egg parasitoid, anagrus nilaparvatae (hymenoptera: mymaridae), of rice planthoppers. | the effects of rice plants infected by rice black streaked dwarf virus (rbsdv) on the host preference, duration of immature stages, sex ratio, and adult longevity and parasitic capacity of an egg parasitoid, anagrus nilaparvatae pang et wang, of rice brown planthopper, nilaparvata lugens stål, were evaluated. tests of response to plant volatiles using an olfactometer showed that a. nilaparvatae preferred rice plants harboring rice brown planthopper eggs over plants free of rice brown planthopper ... | 2014 | 25199055 |
| virus-mediated chemical changes in rice plants impact the relationship between non-vector planthopper nilaparvata lugens stål and its egg parasitoid anagrus nilaparvatae pang et wang. | in order to clarify the impacts of southern rice black-streaked dwarf virus (srbsdv) infection on rice plants, rice planthoppers and natural enemies, differences in nutrients and volatile secondary metabolites between infected and healthy rice plants were examined. furthermore, the impacts of virus-mediated changes in plants on the population growth of non-vector brown planthopper (bph), nilaparvata lugens, and the selectivity and parasitic capability of planthopper egg parasitoid anagrus nilapa ... | 2014 | 25141278 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| synergism between southern rice black-streaked dwarf virus and rice ragged stunt virus enhances their insect vector acquisition. | southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus, family reoviridae, is a novel rice virus transmitted by the white-backed planthopper (sogatella furcifera). since its discovery in 2001, srbsdv has spread rapidly throughout eastern and southeastern asia and caused large rice losses in china and vietnam. rice ragged stunt virus (rrsv) (genus oryzavirus, family reoviridae) is a common rice virus vectored by the brown planthopper (nilaparvata lugens). rr ... | 2014 | 24915431 |
| migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia. | this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ... | 2013 | 24312081 |
| overexpression of rice black-streaked dwarf virus p7-1 in arabidopsis results in male sterility due to non-dehiscent anthers. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper (laodelphax striatellus fallén). rbsdv causes rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. although several rbsdv proteins have been studied in detail, the functions of the nonstructural protein p7-1 are still largely unknown. to investigate the role of the p7-1 protein in v ... | 2013 | 24260239 |
| southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in asia. | southern rice black-streaked dwarf virus (srbsdv), a non-enveloped icosahedral virus with a genome of 10 double-stranded rna segments, is a novel species in the genus fijivirus (family reoviridae) first recognized in 2008. rice plants infected with this virus exhibit symptoms similar to those caused by rice black-streaked dwarf virus. since 2009, the virus has rapidly spread and caused serious rice losses in east and southeast asia. significant progress has been made in recent years in understan ... | 2013 | 24058362 |
| southern rice black-streaked dwarf virus alters insect vectors' host orientation preferences to enhance spread and increase rice ragged stunt virus co-infection. | in recent years, southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus (family reoviridae), has spread rapidly and caused serious rice losses in eastern and southeastern asia. with this virus spread, rice ragged stunt virus (rrsv, genus oryzavirus, family reoviridae) became more common in southern china, usually in co-infection with the former. srbsdv and rrsv are transmitted by two different species of planthoppers, white-backed planthopper (wbph, sogatel ... | 2014 | 24047253 |
| influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper nilaparvata lugens (hemiptera: delphacidae). | rice black streak dwarf virus (rbsdv) is transmitted by the small brown planthopper (sbph), laodelphax striatellus (fallen). non-vector rice brown planthopper (bph), nilaparvata lugens (stål), shares the same host rice plants with sbph in paddy fields. the changes in nutritional composition of rice plants infected by rbsdv and the ecological fitness of bph feeding on the infected plants were studied under both artificial climate chamber and field conditions. contents of 16 detected amino acids a ... | 2014 | 23956237 |
| characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus. | replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ... | 2013 | 23776591 |
| restriction of viral dissemination from the midgut determines incompetence of small brown planthopper as a vector of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a fijivirus, is transmitted by the white-backed planthopper in a persistent-propagative manner. in this study, we found that another planthopper species, the small brown planthopper (sbph), could acquire srbsdv but not transmit it. to identify the transmission barrier for srbsdv in sbphs, sequential infection by srbsdv in the organs of sbphs was studied with immunofluorescence for viral antigens. srbsdv initially entered the epithelial cells of ... | 2012 | 22683297 |
| infection route of rice grassy stunt virus, a tenuivirus, in the body of its brown planthopper vector, nilaparvata lugens (hemiptera: delphacidae) after ingestion of virus. | rice grassy stunt virus (rgsv), a tenuivirus, is transmitted by the brown planthopper (bph), nilaparvata lugens (hemiptera, delphacidae), in a persistent-propagative manner. in this study, immunofluorescence microscopy was used to investigate the infection route of rgsv in the internal organs of bph after acquiring the virus by feeding on rgsv-infected rice plants. the sequential infection study revealed that rgsv initially infected the midgut epithelium, then crossed the basal lamina into the m ... | 2014 | 24768845 |
| development of continuous cell culture of brown planthopper to trace the early infection process of oryzaviruses in insect vector cells. | rice ragged stunt virus (rrsv), an oryzavirus in the family reoviridae, is transmitted by the brown planthopper, nilaparvata lugens, in a persistent-propagative manner. here, we established a continuous cell line of brown planthopper to investigate the mechanism underlying the formation of the viroplasm, the putative site for viral replication and assembly, during infection of rrsv in its insect vector cells. within 24 h of viral infection of cultured cells, the viroplasm had formed and containe ... | 2014 | 24478421 |
| a mitochondrial membrane protein is a target for rice ragged stunt virus in its insect vector. | rice ragged stunt virus (rrsv; reoviridae) is exclusively transmitted by the brown planthopper nilaparvata lugens in a persistent-propagative manner. it is understood that rna viral proliferation is associated with the intracellular membranes of the insect host cells. however, the molecular mechanisms of the interaction between the rrsv proliferation and the intracellular membranes remain essentially unknown. it will be of great interest to determine whether rrsv protein(s) directly interact wit ... | 2017 | 28034779 |
| assembly of the viroplasm by viral non-structural protein pns10 is essential for persistent infection of rice ragged stunt virus in its insect vector. | rice ragged stunt virus (rrsv), an oryzavirus, is transmitted by brown planthopper in a persistent propagative manner. in this study, sequential infection of rrsv in the internal organs of its insect vector after ingestion of virus was investigated by immunofluorescence microscopy. rrsv was first detected in the epithelial cells of the midgut, from where it proceeded to the visceral muscles surrounding the midgut, then throughout the visceral muscles of the midgut and hindgut, and finally into t ... | 2012 | 22837415 |
| evolution of rice stripe virus. | rice stripe virus (rsv) is an insect-borne tenuivirus of economical significance. it is endemic to the rice-growing regions of east asia and exhibits more genetic diversity in yunnan province of china. to gain more insights into the molecular epidemiology and evolution of rsv, recombination analyses were conducted and potential events were detected in each of the four rna segments of rsv. bayesian coalescent method was then applied to the time-stamped coding sequences of the cp gene. the nucleot ... | 2017 | 28189616 |
| rice stripe virus infection alters mrna levels of sphingolipid-metabolizing enzymes and sphingolipids content in laodelphax striatellus. | sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. here we report the identification of 12 sphingolipid genes and their regulation by rice stripe virus in the small brown planthopper (laodelphax striatellus fallén), a serious pest of rice throughout eastern asia. according to protein sequence similarity, we identified 12 sphingolipid enzyme gen ... | 2017 | 28130458 |
| differential proteomics profiling of the ova between healthy and rice stripe virus-infected female insects of laodelphax striatellus. | rice stripe virus-infected females of the small brown planthopper (sbph, laodelphax striatellus) usually lay fewer eggs with a longer hatch period, low hatchability, malformation and retarded or defective development compared with healthy females. to explore the molecular mechanism of those phenomena, we analyzed the differential proteomics profiling of the ova between viruliferous and healthy female insects using an isobaric tag for relative and absolute quantitation (itraq) approach. we obtain ... | 2016 | 27277140 |
| seasonal changes in the percentage of rice stripe virus viruliferous laodelphax striatellus (hemiptera: delphacidae) in paddy fields in japan. | rice stripe disease, which is caused by rice stripe virus (rsv), is one of the most serious viral diseases of rice. rsv is transmitted in a persistent manner bylaodelphax striatellus(fallén). the incidence of the disease can be estimated from the density of viruliferous vectors. understanding seasonal changes of the percentage of viruliferousl. striatelluscan facilitate forecasting and controlling the disease. in paddies, the percentage of viruliferous insects fluctuated in phase with the rate o ... | 2016 | 27099363 |
| viruliferous rate of small brown planthopper is a good indicator of rice stripe disease epidemics. | rice stripe virus (rsv), its vector insect (small brown planthopper, sbph) and climatic conditions in jiangsu, china were monitored between 2002 and 2012 to determine key biotic and abiotic factors driving epidemics of the disease. average disease severity, disease incidence and viruliferous rate of sbph peaked in 2004 and then gradually decreased. disease severity of rsv was positively correlated with viruliferous rate of the vector but not with the population density of the insect, suggesting ... | 2016 | 26898155 |