| development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1. | we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ... | 2009 | 18706944 |
| characterization of duck enteritis virus ul53 gene and glycoprotein k. | abstract: background: most of the previous research work had focused on the epidemiology and prevention of duck enteritis virus (dev). whilst with the development of protocols in molecular biology, nowadays more and more information about the genes of dev was reported. but little information about dev ul53 gene and glycoprotein k(gk) was known except our reported data. results: in our paper, the fluorescent quantitative real-time pcr(fq-rt-pcr) assay and nucleic acid inhibition test were used to ... | 2011 | 21586146 |
| characterization of the gene encoding glycoprotein c of duck enteritis virus. | a total of 2,718 bp of dna fragment was amplified from the c-kce strain of duck enteritis virus (dev) genome using thermal asymmetric interlaced pcr. this newly identified viral dna fragment contained two non-overlapping open reading frames (orfs) oriented from the 5' to 3' direction. the first orf was comprised of 43.5% g + c and contained the full-length genomic sequence of the ul44 gene (1,296 bp) encoding 431 amino acid residues of dev glycoprotein c (gc). the second orf encoded a partial pe ... | 2008 | 18690531 |
| detection of duck plague virus antigen in tissues by immunoperoxidase staining. | an avidin-biotin-peroxidase method of immunoperoxidase staining was successfully adapted for detection of duck plague virus antigen in formalin-fixed, paraffin-embedded tissue sections of the liver and spleen of experimentally infected domestic ducks. positive staining was localized mostly in the nucleus but was also present in the cytoplasm of a few hepatocytes and von kupffer cells of the liver, and lymphocytes and reticular cells of the spleen. | 1993 | 18671026 |
| the ul31 to ul35 gene sequences of duck enteritis virus correspond to their homologs in herpes simplex virus 1. | five orfs in the genome of duck enteritis virus (dev) corresponding to ul31, ul32, ul33, ul34, and ul35 genes of herpes simplex virus 1 (hsv-1) were amplified by a modified "targeted gene walking" pcr, cloned, and sequenced. ul33, ul34, and ul35 genes were oriented from the left to the right of genome, while ul31 and ul32 had an opposite orientation. a comparison of deduced amino acid sequences of the dev orfs with their alphaherpesvirus homologs showed well-conserved regions except for the ul34 ... | 2008 | 18459832 |
| molecular characterization of the herpes simplex virus 1 (hsv-1) homologues, ul25 to ul30, in duck enteritis virus (dev). | a 16.6-kilo-base pair (kb) sequence was amplified from the duck enteritis virus (dev) clone-03 strain genome using 'targeted gene walking polymerase chain reaction (pcr)'. seven complete open reading frames (orfs) were predicted, and designated herpes simplex virus 1 (hsv-1) homologues, unique long (ul) 25, ul26, ul26.5, ul27, ul28, ul29, and ul30. sequence analysis revealed that the arrangement of seven genes in dev clone-03 strain was collinear to that from hsv-1. in addition, mrna transcripti ... | 2007 | 17706377 |
| complete genome sequence of virulent duck enteritis virus (dev) strain 2085 and comparison with genome sequences of virulent and attenuated dev strains. | we here report the complete genome sequence of the duck enteritis virus (dev) wild-type strain 2085, an avian herpesvirus (genbank id: jf999965). the nucleotide sequence was derived from the 2085 genome cloned as an infectious bacterial artificial chromosome (bac) clone. the dev 2085 genome is 160,649-bp in length and encodes 78 predicted open reading frames (orfs), a number identical to that identified for the attenuated dev vac strain (genbank id: eu082088.2). comparison of the genome sequence ... | 2011 | 21802458 |
| A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus infection in ducks. | Ducks play an important role in the maintenance of highly pathogenic H5N1 avian influenza viruses (AIVs) in nature, and the successful control of AIVs in ducks has important implications for the eradication of the disease in poultry and its prevention in humans. The inactivated influenza vaccine is expensive, labor-intensive, and usually needs 2 to 3 weeks to induce protective immunity in ducks. Live attenuated duck enteritis virus (DEV; a herpesvirus) vaccine is used routinely to control lethal ... | 2011 | 21865383 |
| Expression and characterization of UL16 gene from duck enteritis virus. | Previous studies have indicated that the UL16 protein and its homologs from herpesvirus were conserved and played similar roles in viral DNA packaging, virion assembly, budding, and egress. However, there was no report on the UL16 gene product of duck enteritis virus (DEV). In this study, we analyzed the amino acid sequence of UL16 using bioinformatics tools and expressed in Escherichia coli Rosetta (DE3) induced by isopropy1-ß-D-thiogalactopyranoside (IPTG). The recombinant protein was produced ... | 2011 | 21861926 |
| preliminary study on duck enteritis virus-induced lymphocyte apoptosis in vivo. | we studied apoptosis induced by duck enteritis virus (dev) in vivo, focusing on the lymphoid organs that constitute the main targets for infection: thymus, bursa of fabricius (bf), and spleen. fifty pekin ducks were inoculated subcutaneously with a virulent strain of dev. the morphology of lymphoid organs of these infected ducks was observed by light microscopy and transmission electron microscopy. cell death by classical necrosis was observed in lymphocytes of the dev-infected thymus, bf, and s ... | 2007 | 17626481 |
| comprehensive mapping of common immunodominant epitopes in the eastern equine encephalitis virus e2 protein recognized by avian antibody responses. | eastern equine encephalitis virus (eeev) is a mosquito-borne virus that can cause both human and equine encephalitis with high case fatality rates. eeev can also be widespread among birds, including pheasants, ostriches, emu, turkeys, whooping cranes and chickens. the e2 protein of eeev and other alphaviruses is an important immunogenic protein that elicits antibodies of diagnostic value. while many therapeutic and diagnostic applications of e2 protein-specific antibodies have been reported, the ... | 2013 | 23922704 |
| comprehensive mapping of common immunodominant epitopes in the west nile virus nonstructural protein 1 recognized by avian antibody responses. | west nile virus (wnv) is a mosquito-borne flavivirus that primarily infects birds but occasionally infects humans and horses. certain species of birds, including crows, house sparrows, geese, blue jays and ravens, are considered highly susceptible hosts to wnv. the nonstructural protein 1 (ns1) of wnv can elicit protective immune responses, including ns1-reactive antibodies, during infection of animals. the antigenicity of ns1 suggests that ns1-reactive antibodies could provide a basis for serol ... | 2012 | 22347477 |
| efficient strategy to generate a vectored duck enteritis virus delivering envelope of duck tembusu virus. | duck tembusu virus (dtmuv) is a recently emerging pathogenic flavivirus that has resulted in a huge economic loss in the duck industry. however, no vaccine is currently available to control this pathogen. consequently, a practical strategy to construct a vaccine against this pathogen should be determined. in this study, duck enteritis virus (dev) was examined as a candidate vaccine vector to deliver the envelope (e) of dtmuv. a modified mini-f vector was inserted into the sorf3 and us2 gene junc ... | 2014 | 24956180 |
| live attenuated vaccine based on duck enteritis virus against duck hepatitis a virus types 1 and 3. | as causative agents of duck viral hepatitis, duck hepatitis a virus type 1 (dhav-1) and type 3 (dhav-3) causes significant economic losses in the duck industry. however, a licensed commercial vaccine that simultaneously controls both pathogens is currently unavailable. here, we generated duck enteritis virus recombinants (rc-kce-2vp1) containing both vp1 from dhav-1 (vp1/dhav-1) and vp1 from dhav-3 (vp1/dhav-3) between ul27 and ul26. a self-cleaving 2a-element of fmdv was inserted between the tw ... | 2016 | 27777571 |
| role of virus-encoded micrornas in avian viral diseases. | with total dependence on the host cell, several viruses have adopted strategies to modulate the host cellular environment, including the modulation of microrna (mirna) pathway through virus-encoded mirnas. several avian viruses, mostly herpesviruses, have been shown to encode a number of novel mirnas. these include the highly oncogenic marek's disease virus-1 (26 mirnas), avirulent marek's disease virus-2 (36 mirnas), herpesvirus of turkeys (28 mirnas), infectious laryngotracheitis virus (10 mir ... | 2014 | 24662606 |
| development of quantitative real-time polymerase chain reaction for duck enteritis virus dna. | duck enteritis virus (dev) is a herpesvirus that causes an acute, contagious, and fatal disease. in the present article, we introduce a quantitative real-time polymerase chain reaction (pcr) assay for dev dna using taqman technology and a two-step protocol. it was confirmed to be rapid, sensitive, and specific for dev detection. the primers and probe were designed and directed to the dna polymerase gene of dev. the method will provide a valuable tool for rapid laboratory diagnosis of dev infecti ... | 2005 | 16252495 |
| a one-step duplex rrt-pcr assay for the simultaneous detection of duck hepatitis a virus genotypes 1 and 3. | duck hepatitis a virus (dhav) is a highly infectious pathogen that causes significant bleeding lesions in the viscera of ducklings less than 3 weeks old. there are three serotypes of dhav: serotype 1 (dhav-1), serotype 2 (dhav-2) and serotype 3 (dhav-3). these serotypes have no cross-antigenicity with each other. to establish an rrt-pcr assay for the rapid detection of a mixed infection of dhav-1 and dhav-3, two pairs of primers and a pair of matching taqman probes were designed based on conserv ... | 2016 | 27435338 |
| electron microscopic studies of the morphogenesis of duck enteritis virus. | the morphogenesis of duck enteritis virus (dev) and distribution in vivo were observed by electron microscopy after ducks were infected experimentally with dev virulent strain. the investigation showed that a few typical herpesvirus virions and nucleocapsids were first observed in the spleen, thymus, and bursa of fabricius (bf), and many nucleocapsids, mature viruses, and viral inclusion bodies could be found in the nucleus and cytoplasm of infected liver, small intestine, spleen, thymus, and bf ... | 2005 | 15839412 |
| construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain. | duck enteritis virus (dev) is the causative agent of duck viral enteritis, which causes an acute, contagious and lethal disease of many species of waterfowl within the order anseriformes. in recent years, two laboratories have reported on the successful construction of dev infectious clones in viral vectors to express exogenous genes. the clones obtained were either created with deletion of viral genes and based on highly virulent strains or were constructed using a traditional overlapping fosmi ... | 2013 | 24195756 |
| characterization of the duck plague virus ul35 gene. | previous study has demonstrated that the duck plague virus (dpv) ul35 gene can be expressed as a recombinant fusion protein, and the prepared antiserum has a high reactivity and specificity against the purified recombinant protein. in the present study, to elucidate the properties and functions of its encoding protein, the ul35 gene product (vp26) was identified by using the prepared rabbit polyclonal antiserum. | 2010 | 20606463 |
| cloning, expression and characterization of ge protein of duck plague virus. | the ge protein of duck plague virus is the important membrane glycoprotein, its protein characterization has not been reported. in this study, we expressed and presented the characterization of the dpv ge product. | 2010 | 20529349 |
| recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. | to design an alternative vaccine for control of infectious bronchitis in chickens, three recombinant duck enteritis viruses (rdevs) expressing the n, s, or s1 protein of infectious bronchitis virus (ibv) were constructed using conventional homologous recombination methods, and were designated as rdev-n, rdev-s, and rdev-s1, respectively. chickens were divided into five vaccinated groups, which were each immunized with one of the rdevs, covalent vaccination with rdev-n & rdev-s, or covalent vacci ... | 2016 | 26946113 |
| [cloning, prokaryotic expression and subcellular localization in the infected host cells of the duck plague virus dpv ul35 gene]. | based on the duck plague virus (dpv) ul35 gene sequence that our laboratory obtained (genbank accession number ef643558), a pair of primers was designed using oligo6.0 and primer5.0, then the ul35 gene was amplified from dpv chv strain genomic dna and cloned into the pmd18-t to construct a clone plasmid pmd18-t-ul35. after identification of the pmd18-t-ul35 by pcr amplification and restriction digestion, the fragment of the ul35 gene was subcloned into the prokaryotic expression vector pet-32a(+ ... | 2010 | 20480644 |
| adaptation and growth kinetics study of an indian isolate of virulent duck enteritis virus in vero cells. | duck virus enteritis, also known as duck plague, is a viral infection of ducks caused by duck enteritis virus (dev). the control of the disease is mainly done by vaccination with chicken embryo adapted live virus that is known to be poorly immunogenic and elicits only partial protection. further, the embryo propagated vaccine virus pose a threat of harboring other infectious agents. seeing these limitations, the present study reports for the first time regarding propagation and adaptation of a v ... | 2015 | 25450886 |
| complete genome sequence of an attenuated duck enteritis virus obtained by in vitro serial passage. | here, we present the complete genome sequence of an attenuated duck enteritis virus (dev) obtained by serial chicken embryo passage. compared with a virulent dev, there is a serial deletion in unique long open reading frame 11 (lorf11) and unique long region 2 (ul2). this study will aid in further exploration of the molecular pathogenesis of dev. | 2013 | 24009119 |
| glycoprotein c plays a role in the adsorption of duck enteritis virus to chicken embryo fibroblasts cells and in infectivity. | unlike glycoprotein c (gc) of many mammalian herpes viruses, gc of some avian herpes viruses does not play a principle role in the binding of virus to heparin sulfate proteoglycans on the cell surface. the roles of duck enteritis virus (dev) gc on viral attachment remained unclear. in this study, we showed that gc expressed in vitro could bind to chicken embryo fibroblasts (cef) cells and inhibit the adsorption of duck enteritis virus (dev) onto cef cells effectively and antiserum directed again ... | 2013 | 23454009 |
| novel micrornas encoded by duck enteritis virus. | duck enteritis virus (dev) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. using a deep sequencing approach on rna from infected chicken embryo fibroblast cultures, we identified several novel dev-encoded micro (mi)rnas. unlike most mardivirus-encoded mirnas, dev-encoded mirnas mapped mostly to the unique long region of the genome. the precursors of dev mir-d18 and mir-d19 overlapped with each other, suggesting similarities to mirna-off ... | 2012 | 22492913 |
| a multiplex pcr for detection of six viruses in ducks. | in this study, six pairs of specific primers that can amplify dna fragments of different sizes were designed and synthesized according to viral protein gene sequences published in genbank. then, a multiplex pcr method was established for rapid detection of duck hepatitis virus 1, duck plague virus, duck tembusu virus, muscovy duck parvovirus, muscovy duck reovirus, and duck h9n2 avian influenza virus, and achieve simple and rapid detection of viral diseases in ducks. single pcr was used to confi ... | 2017 | 28728835 |
| characterization of duck enteritis virus us6, us7 and us8 gene. | duck enteritis virus (dev) is a member of the family herpesviridae, the genome of which is not completely analyzed. in order to provide further insights into the genomic organization, we decided to amplify the unknown sequence in the us region since three key glycoproteins are coded in this region. glycoprotein d encoded by us6 gene is essential for virus entry and plays an important role in protecting animals from lethal challenge. glycoprotein i and e encoded by us7 and us8 gene are required f ... | 2010 | 20090359 |
| duck stimulator of interferon genes plays an important role in host anti-duck plague virus infection through an ifn-dependent signalling pathway. | the human stimulator of interferon gene (sting) is an important molecule in innate immunity that stimulates type i interferon (ifn) production. however, the role of duck sting (dusting) in innate immunity has yet to be explained. in this study, the full length of the dusting cdna sequence (1149bp), which encodes 382 amino acid (aa) residues, was reported and showed the highest sequence similarity with chicken stings. the phylogenetic analysis based on sting aa showed that dusting was grouped ont ... | 2018 | 28969942 |
| microbiological identification and analysis of waterfowl livers collected from backyard farms in southern china. | in total, 985 livers were collected from 275 backyard waterfowl farms distributed in seven provinces of southern china. the virus that was most commonly isolated was avian influenza virus, with a 12.1% positivity rate. of the other positive samples, 10.6% tested positive for avian tembusu virus, 6.8% for duck hepatitis a virus, 3.8% for duck plague virus, 3.4% for muscovy duck parvovirus, 3.1% for goose parvovirus, 1.0% for mycoplasma, and 0.9% for respiratory enteric orphan virus. the bacterium ... | 2018 | 29398671 |
| cloning, expression, purification and characterization of ul24 partial protein of duck enteritis virus. | the ul24 gene of duck enteritis virus (dev) is conserved across herpesviruses, but its protein characterization has not been reported. we expressed the ul24 gene in escherichia coli bl21 from a recombinant plasmid pet32a/dev-ul24 and used the resulting protein to raise antiserum. this antiserum recognized a 38-kda protein in lysates from infected cells. sds-page analysis showed that the ul24 partial protein was highly expressed after induction by 0.4 mm iptg at 30 degrees for 6 h. the results of ... | 2009 | 19786809 |
| optimized expression of duck tembusu virus e gene delivered by a vectored duck enteritis virus in vitro. | in our previous study, a recombinant duck enteritis virus (dev) delivering codon-optimized e gene (named as e-ch) of duck tembusu virus (dtmuv) optimized referring to chicken's codon bias has been obtained based on the infectious bacterial artificial chromosome (bac) clone of duck enteritis virus vaccine strain pdev-ef1, but the expression level of e-ch in recombinant virus rdev-e-ch-infected cells was very low. to optimize dtmuv e gene expression delivered by the vectored dev, different forms o ... | 2019 | 31482466 |
| molecular cloning and functional characterization of duck tyk2. | tyrosine kinase 2 (tyk2), a member of janus kinase family, has been identified as a crucial protein in signal transduction initiated by interferons or interleukins in mammals. however, the function of avian tyk2 in innate immune response remains largely unknown. in this study, the full-length duck tyk2 (dutyk2) cdna was cloned for the first time, which encoded a putative protein of 1187 amino acid residues and showed the high sequence similarity with bald eagle, crested ibis, and white-tailed tr ... | 2020 | 31437526 |
| characterization of synonymous codon usage bias in the duck plague virus ul35 gene. | the aim was to identify the codon usage bias between the newly identified duck plague virus (dpv) ul35 gene (genbank accession no. ef643558) and the ul35-like genes of 27 other reference herpesviruses. | 2009 | 19672100 |
| expression and distribution of the duck enteritis virus ul51 protein in experimentally infected ducks. | to determine the expression and distribution of tegument proteins encoded by duck enteritis virus (dev) ul51 gene in tissues of experimentally infected ducks, for the first time, an immunoperoxidase staining method to detect ul51 protein (ul51p) in paraffin-embedded tissues is reported. a rabbit anti-ul51 polyclonal serum, raised against a recombinant 6-his-ul51 fusion protein expressed in escherichia coli, was prepared, purified, and used as primary antibodies. fifty-eight 30-day-old dev-free d ... | 2010 | 20608544 |
| expressing gk gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis. | duck viral enteritis, which is caused by duck enteritis virus (dev), causes significant economic losses in domestic and wild waterfowls because of the high mortality and low egg production rates. with the purpose of eliminating this disease and decreasing economic loss in the commercial duck industry, researching on glycoprotein k (gk) of dev may be a new kind of method for preventing and curing this disease. because glycoproteins project from the virus envelope as spikes and are directly involv ... | 2010 | 20663161 |
| molecular and pathological characterization of duck enteritis virus in egypt. | in winter 2016, a fatal disease outbreak suspected to be duck virus enteritis (dve) stroke over a million ducklings in 10 white pekin and muscovy ducks flocks in dakahlia and gharbia governorates, egypt, causing heavy economic losses. the disease quickly killed 20%-60% of affected farms. the clinical signs were inappetence, ataxia, crowding in corners, partially closed eye lids and blue beaks. post mortem examination revealed white necrotic foci in liver, mottled spleen and sometimes cecal core. ... | 2019 | 30144300 |
| recombinant duck enteritis viruses expressing the newcastle disease virus (ndv) f gene protects chickens from lethal ndv challenge. | newcastle disease virus (ndv) is a major threat to poultry worldwide. virulent newcastle disease virus infection can cause 100% morbidity and mortality in chickens. vaccination is the most effective way to prevent and control ndv outbreaks in poultry. previously, we demonstrated that a duck enteritis virus (dev) vaccine strain is a promising vector to generate recombinant vaccines in chickens. here, we constructed two recombinant devs expressing the f protein (rdev-f) or hn protein (rdev-hn) of ... | 2019 | 31030839 |
| development and application of an indirect immunohistochemical method for the detection of duck plague virus vaccine antigens in paraffin sections and localization in the vaccinated duckling tissues. | the objective of the present study was to develop and apply a streptavidin-alkaline phosphatase labeling system of indirect immunohistochemistry (sp-ihc) to detect antigenic distribution and localization regularity of duck plague virus (dpv) vaccine antigens in paraformaldehyde-fixed paraffin-embedded tissues of experimentally vaccinated ducklings. male new zealand rabbits were immunized with purified dpv antigens, which were engaged by a combination of differential centrifugation and sucrose-de ... | 2010 | 20709976 |
| the pathogenesis of a north american h5n2 clade 2.3.4.4 group a highly pathogenic avian influenza virus in surf scoters (melanitta perspicillata). | aquatic waterfowl, particularly those in the order anseriformes and charadriiformes, are the ecological reservoir of avian influenza viruses (aivs). dabbling ducks play a recognized role in the maintenance and transmission of aivs. furthermore, the pathogenesis of highly pathogenic aiv (hpaiv) in dabbling ducks is well characterized. in contrast, the role of diving ducks in hpaiv maintenance and transmission remains unclear. in this study, the pathogenesis of a north american a/goose/1/guangdong ... | 2020 | 32967673 |
| proteomic analysis of host cellular proteins co-immunoprecipitated with duck enteritis virus gc. | duck enteritis virus (dev), the causative agent of duck viral enteritis, causes a contagious, lethal viral disease in anseriformes (waterfowls). in virus infection, host-virus interaction plays a crucial role in virus replication and pathogenesis. in our previous study, mrfp was fused with the c-terminus of dev glycoprotein c (gc) to construct a fluorescent-tag dev virus rgcrfp. in the current study, fluorescent fusion protein (gc-mrfp) was used as the proteomic probe. co-immunoprecipitation and ... | 2021 | 34091090 |
| simultaneous tracking of capsid vp26, envelope protein gc localization in living cells infected with double fluorescent duck enteritis virus. | duck enteritis virus (dev) can cause an acute, contagious and lethal disease of many species of waterfowl. an infectious bacterial artificial chromosome clone of dev vaccine strain pe1 (pdev-ef1) has been constructed in our previous study. based on pe1, a recombinant mutated clone pdl (pvp26cfp-gcrfp), which carries a red fluorescent protein (mrfp) gene fused to the viral envelope protein gc in combination with a cyan fluorescent protein (cfp) gene fused to the viral capsid vp26, was constructed ... | 2021 | 33727092 |
| identification of a novel linear b-cell epitope in the ul26 and ul26.5 proteins of duck enteritis virus. | the unique long 26 (ul26) and ul26.5 proteins of herpes simplex virus are known to function during the assembly of the viruses. however, for duck enteritis virus (dev), which is an unassigned member of the family herpesviridae, little information is available about the function of the two proteins. in this study, the c-terminus of dev ul26 protein (designated ul26c), which contains the whole of ul26.5, was expressed, and the recombinant ul26c protein was used to immunize balb/c mice to generate ... | 2010 | 20836860 |
| transcription phase, protein characteristics of dev ul45 and prokaryotic expression, antibody preparation of the ul45 des-transmembrane domain. | some ul45 gene function of herpesvirus was reported. while there was no any report of the duck enteritis virus (dev) ul45 protein as yet. | 2010 | 20843372 |
| functional characterization of the cc chemokine rantes from pekin duck (anas platyrhynchos). | rantes (regulated upon activation, normal t-cell expressed and secreted) is a key pro-inflammatory cytokine that belongs to the cc-group of chemokines. the present study was carried out to functionally characterize the previously identified rantes homologue in domestic duck (genbank accession no. ay641435). recombinant duck rantes was expressed in escherichia coli-based and hek293t cell-based systems. a trna supplementation strategy was required to express the protein in e. coli due to the prese ... | 2011 | 20850473 |
| immunofluorescence analysis of duck plague virus ge protein on dpv-infected ducks. | in previous studies, the expression and localization characteristics of duck plague virus (dpv) ge protein have been described in cultured cells, but the properties of dpv ge protein have not been reported in vivo. immunofluorescence analysis had been used for the detection of virus antigen, but there was no report on the use of this technique for the detection of dpv ge. in this study, we investigated the distribution of dpv ge protein on dpv-infected ducks using polyclonal antibody raised agai ... | 2011 | 21235807 |
| expression and characterization of recombinant vp19c protein and n-terminal from duck enteritis virus. | previous studies have indicated that the vp19c protein and its homology play similar roles in capsid assembly of all alphaherpesvirus subfamily. however, there is no report on the vp19c protein of duck enteritis virus (dev). in this study, we expressed the dev vp19c protein and presented its antigenic properties. moreover, we developed polyclonal antibody against the vp19c protein and characterized it. | 2011 | 21349183 |
| expression and immunohistochemical distribution of duck plague virus glycoprotein ge in infected ducks. | to determine the distribution of duck plague virus (dpv) ge protein in paraformaldehyde-fixed, paraffin-embedded tissues of experimentally dpv-infected ducks, an indirect immunoperoxidase assay was established to detect glycoprotein e (ge) protein for the first time. the rabbit anti-his-ge serum, raised against the recombinant his-ge fusion protein expressed in escherichia coli bl21 (de3), was prepared and purified. western blotting and indirect immunofluorescence analysis showed that the anti-h ... | 2011 | 21500643 |
| generation of an infectious clone of duck enteritis virus (dev) and of a vectored dev expressing hemagglutinin of h5n1 avian influenza virus. | we report on the generation of an infectious bacterial artificial chromosome (bac) clone of duck enteritis virus (dev) and a vectored dev vaccine expressing hemagglutinin (h5) of high pathogenicity h5n1 avian influenza virus (aiv). for generation of the dev bac, we inserted mini-f vector sequences by homologous recombination in lieu of the ul44 (gc) gene of dev isolate 2085. dna of the resulting in recombinant virus v2085-gfpδgc was electroporated into escherichia coli and a full-length dev bac ... | 2011 | 21549165 |
| induction of immune responses in ducks with a dna vaccine encoding duck plague virus glycoprotein c. | abstract: background: a dna vaccine expressing glycoprotein c (gc) of duck plague virus (dpv) was evaluated for inducing immunity in ducks. the plasmid encoding gc of dpv was administered via intramuscular (im) injection and gene gun bombardment. results: after immunization by both routes virus-specic serum antibody and t-cell responses developed. vaccination of ducks by im injection induced a stronger humoral, but weaker cell-mediated immune response. in contrast, a better cell-mediated immune ... | 2011 | 21569289 |
| expression and characterization of duck enteritis virus gi gene. | abstract: background: at present, alphaherpesviruses gi gene and its encoding protein have been extensively studied. it is likely that gi protein and its homolog play similar roles in virions direct cell-to-cell spread of alphaherpesviruses. but, little is known about the characteristics of dev gi gene. in this study, we expressed and presented the basic properties of the dev gi protein. results: the special 1221-bp fragment containing complete open reading frame(orf) of duck enteritis virus(dev ... | 2011 | 21595918 |
| characterization of the duck enteritis virus ul55 protein. | abstract: background: characteration of the newly identified duck enteritis virus ul55 gene product has not been reported yet. knowledge of the protein ul55 can provide useful insights about its function.results:the newly identified duck enteritis virus ul55 gene was about 561 bp, it was amplified and digested for construction of a recombinant plasmid pet32a(+)/ul55 for expression in escherichia coli. sds-page analysis revealed the recombinant protein ul55(pul55) was overexpressed in escherichia ... | 2011 | 21609474 |
| establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus ul55 gene. | abstract: background: real-time quantitative reverse transcription polymerase chain reaction assay (qrt-pcr) has become the benchmark for detection and quantication of target gene expression level and been utilized increasingly in detection of viral load and therapy monitoring. the dynamic transcription variation of duck enteritis virus ul55 gene during the life cycle of duck enteritis virus in infected cells has not been reported yet.results:the newly identified duck enteritis virus ul55 gene w ... | 2011 | 21631934 |
| comparative analysis of the genes ul1 through ul7 of the duck enteritis virus and other herpesviruses of the subfamily alphaherpesvirinae. | the nucleotide sequences of eight open reading frames (orfs) located at the 5' end of the unique long region of the duck enteritis virus (dev) clone-03 strain were determined. the genes identified were designated ul1, ul2, ul3, ul4, ul5, ul6 and ul7 homologues of the herpes simplex virus 1 (hsv-1). the dev ul3.5 located between ul3 and ul4 had no homologue in the hsv-1. the arrangement and transcription orientation of the eight genes were collinear with their homologues in the hsv-1. phylogeneti ... | 2009 | 21637656 |
| recombinant duck enteritis virus works as a single-dose vaccine in broilers providing rapid protection against h5n1 influenza infection. | although vaccination is an important strategy for controlling h5n1 avian influenza virus infections, broilers (short-lived meat chickens) remain the major victims in disease-endemic countries. inactivated vaccine usually requires 2-3weeks to establish solid protection, and recombinant vaccines, including the recombinant fowlpox virus vaccine and the recombinant newcastle disease virus vaccine are affected by maternal antibodies against the vectors. these disadvantages compromise the protective e ... | 2012 | 23267833 |
| astroviruses associated with stunting and pre-hatching mortality in duck and goose embryos. | the first detection of avian nephritis virus (anv) in goose embryos and of turkey astrovirus-1 (tastv-1) in duck embryos is described. intestinal samples from duck and goose embryos from five duck and four goose flocks in croatia were tested by polymerase chain reaction for the presence of anv, tastv-1, turkey astrovirus-2, chicken astrovirus, duck astrovirus and also for the presence of avian reovirus, derzsy's disease virus and duck enteritis virus. the kidneys from duck and goose embryos were ... | 2012 | 22845326 |
| gexp analyzer-based multiplex reverse-transcription pcr assay for the simultaneous detection and differentiation of eleven duck viruses. | duck viral pathogens primarily include the avian influenza virus (aiv) subtypes h5, h7, and h9; duck hepatitis virus (dhv); duck tembusu virus (dtmuv); egg drop syndrome virus (edsv); duck enteritis virus (dev); newcastle disease virus (ndv); duck circovirus (ducv); muscovy duck reovirus (mdrv); and muscovy duck parvovirus (mdpv). these pathogens cause great economic losses to china's duck breeding industry. | 2015 | 26518004 |
| recombinant duck enteritis virus expressing the ha gene from goose h5 subtype avian influenza virus. | the duck enteritis virus (dev) may be a promising candidate viral vector for an aquatic poultry vaccination that can protect against multiple pathogens because it has a very large genome and a narrow host range. recently, we described two dev recombinants that contained deletions of the viral us2 or gige genes. the hemagglutinin (ha) gene of an h5n1-type highly pathogenic avian influenza virus (hpaiv) of goose origin was inserted into the deletion sites to construct two rdevs expressing the aiv ... | 2013 | 24144474 |
| first survey of the occurrence of duck enteritis virus (dev) in free-ranging polish water birds. | duck plague (dp) caused by anatid herpesvirus 1, also called duck enteritis virus (dev), presents one of the most important concerns in mass waterfowl production. apart from geese and ducks, free-ranging water birds are the most notorious infection carriers. the epidemiology of dev in western europe remains unknown. therefore, it was reasonable to conduct a study on its occurrence using modern but simple real-time loop-mediated isothermal amplification (lamp). analysis of 132 field isolates show ... | 2014 | 24327092 |
| a polymerase chain reaction assay for detection of virulent and attenuated strains of duck plague virus. | sequence analysis of duck plague virus (dpv) revealed that there was a 528bp (b fragment) deletion within the ul2 gene of dpv attenuated vaccine strain in comparison with field virulent strains. the finding of gene deletion provides a potential differentiation test between dpv virulent strain and attenuated strain based on their ul2 gene sizes. thus we developed a polymerase chain reaction (pcr) assay targeting to the dpv ul2 gene for simultaneous detection of dpv virulent strain and attenuated ... | 2017 | 28860100 |
| molecular cloning and functional characterization of duck janus kinase 1. | janus kinase 1 (jak1) is a member of jak family of non-receptor protein tyrosine kinases that plays critical roles in transducing cytokine signals via jak-signal transducer and activator of transcription (stat) signaling pathway. the importance of jak1 in innate immunity has been well-studied in mammals and fish, yet in avian remains largely unknown. here, we cloned the full-length of the duck jak1 (dujak1) gene for the first time. dujak1 encoded a protein of 1152 amino acids and possessed high ... | 2020 | 31733446 |
| development of a simple and rapid immunochromatographic strip test for detecting duck plague virus antibodies based on gi protein. | a colloidal gold strip (cgs) for detecting antibodies to duck plague virus (dpv) was developed. colloidal gold-labeled dpv gi protein and goat anti-rabbit igg were dispensed on a conjugate pad as tracers. the recombinant dpv gi protein and rabbit igg were used as capture reagents at the test line and control line, respectively. the detection limit of this assay was 1:256. additionally, the cgs did not react with antisera from other common duck diseases, only reacting with anti-dpv serum and yiel ... | 2020 | 31863862 |
| galectin-1 induces immune response and antiviral ability in cherry valley ducks after duck plague virus infection. | galectin-1, as a typical animal galactose-binding protein, it is found on the cell surface and in the extracellular matrix. cloning the full-length coding sequence of galectin-1 from the spleens of cherry valley ducks revealed that the coding sequence of duck galectin-1 (dugal-1) comprises 405 bp, encoding 134 amino acids. homologic analysis revealed its amino acid sequence is most identical to that of anas platyrhynchos (98.8%) followed by gallus gallus. quantitative real-time pcr analysis indi ... | 2019 | 30986433 |
| development of a pcr assay for detection and differentiation of muscovy duck and goose parvoviruses based on ns gene characterization. | muscovy duck parvovirus (mdpv) and goose parvovirus (gpv) have both been found to cause high mortality and morbidity in muscovy ducklings. specific detection is often rife with false positives due to high identity at the genomic nucleotide level and antigenic similarity between mdpvs and gpvs. in this study, significantly variable regions were found, via non-structural (ns) comparison, between mdpv and gpv ns genes; however, ns genes were conserved within the mdpv and gpv groups. a polymerase ch ... | 2018 | 30298830 |
| molecular cloning and functional characterization of duck ddx41. | dead (asp-glu-ala-asp) box polypeptide 41 (ddx41), a receptor belonging to dexd/h-box helicase family, acts as an intracellular dna sensor and induces type i ifn production in mammals and fish. however, the function of avian ddx41 in innate immune response is still unknown. in this study, the full-length duck ddx41 (duddx41) cdna sequence was cloned for the first time and encoded a putative protein of 618 amino acid residues which showed the high sequence similarity with both zebra finch and chi ... | 2018 | 30025984 |
| construction of a recombinant duck enteritis virus (dev) expressing hemagglutinin of h5n1 avian influenza virus based on an infectious clone of dev vaccine strain and evaluation of its efficacy in ducks and chickens. | highly pathogenic avian influenza virus (aiv) subtype h5n1 remains a threat to poultry. duck enteritis virus (dev)-vectored vaccines expressing aiv h5n1 hemagglutinin (ha) may be viable aiv and dev vaccine candidates. | 2015 | 26263920 |