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plasma levels of luteinizing hormone in gonadectomized japanese quail exposed to short or to long daylengths.plasma levels of luteinizing hormone (lh) were measured by radioimmunoassay in gonadectomized male and female japanese quail, exposed either to 8 h light: 16 h darkness per day (8l:16d; short days) or to 20l:4d (long days). in both sexes, exposure to long days increased lh levels and in the gonadectomized quail lh continued to rise over several weeks. eventually the castrated quail had levels about five times higher than the control birds and the ovariectomized quail had levels about 14 times hi ...19751117231
chicken gonadotrophins: their effects on the testes of immature and hypophysectomized japanese quail.five-week old japanese quail held under short daylengths (8l/16d) were injected daily for two weeks with 20 mug/day of purified chicken luteinizing hormone (lh) or with one of two chicken follicle-stimulating hormone (fsh) fractions. both intact and hypophysectomized birds were used. after killing, the testes were weighed and fixed for light- and electron-microscopy. following hypophysectomy testicular weights fell significantly below those of saline-injected controls, indicating that short day ...19751120336
the effect of mirex on reproduction of japanesequail and on characteristics of eggs from japanese quail and chickens.laying white leghorn chickens were fed mirex at 0, 5, 10, 20, 40, 80, and 160 ppm for 12 weeks, and laying japanese quail were fed mirex at 0, 5, 40, and 80 ppm for 12weeks. the data suggest that dietary mirex at these levels did not affect egg production, egg weight, shell thickness, shell calcium, the proportion of broken eggs, or the proportion of soft-shelled eggs of either chickens or quail. statistical significance (p greater than 0.05) associated with dietary mirex was detected in the ana ...19751130832
ontogeny of lactate dehydrogenase isozymes in chicken-quail hybrid embryos.the ontogeny of lactate dehydrogenase (ldh) isozymes was examined in avian hybrids and compared with the isozyme patterns of the parental species. hybrids were obtained by crossing female japanese quali (coturnix coturnix japonica) with male domestic chickens (gallus gallus domesticus). by use of starch gel electrophoresis and an enzyme-specific stain, traces of embryonic paternally derived ldh were detected in unincubated hybrid eggs. it was concluded that the embryonic genes coding for the b s ...19751137566
rate of thyroxine secretion by male and laying japanese quail: identification of the radioactive thyroxine degradation component of the multiphasic 131-i curve.counting of radioactivity in japanese quail in vivo showed a rapid loss of 131-i from the body 12-24 h after the i.v. injection of [131-i]thyroxine (t4), followed by a period of slow decrease in counting rates to 96 h. from comparison of these [131-i]t4 curves with curves for 131-iodide-injected birds and from counts on serum and other tissues in vitro it was concluded that, for japanese quail, the t4 secretion rate should be calculated using serum samples taken during the first 12 h. using this ...19751141813
nitrosobenzene-induced ferrihemoglobin formation in japanese quail erythrocytes. the significance of ferrihemoglobin reduction.quantitative aspects of nitrosobenzene-induced ferrihemoglobin formation were studied in quail erythrocytes in comparison to goat red cells. many similarities exist with the mechanism in mammalian red cells such as a nadph-dependent enzymatic recycling of phenylhydroxylamine from nitrosobenzene. also the mechanism of ferrihemoglobin reduction is similar in both species, it is a nadh-dependent process. however, the reducing activity of quail red cells exceeds by far the activity of the majority o ...19751165790
selection for mating ability in japanese quail.results are presented for a replicated mass selection experiment for high and low cumulative number of completed matings. through six generations of selection heritabilities of the selected trait averaged .07 for the high lines and .25 for the low lines. no correlated responses were noted for body weight at either 4 or 8 weeks of age, whereas the relationships of the selected trait with size of the cloacal gland and relative aggressiveness were positive.19751171460
[reaction to vaccination and measles antibody levels in children inoculated with vaccine prepared from the l-16 strain from japanese quail embryonic fibroblast cultures]. 19751178922
gene activation of alcohol dehydrogenase in japanese quail and chicken-quail hybrid embryos.no preferential activation of the maternally derived alcohol dehydrogenase (adh) allele was found in any of the chicken male x japanese quail female hybrids examined. adh activity in the liver was, in fact, found to exist in two different cathodal zonal regions on starch gel electropherograms; the zone ii bands appeared at day 5 of incubation in the quail embryo (day 6 in the hybrid embryo) and the zone i bands appeared in 9-day quail embryos (10-day hybrid embryos). by day 13 of incubation, onl ...19751180882
effects of the egg cycle and route of administration of prostaglandin-induced oviposition of hens and japanese quail. 19751181427
enzymes and electrolytes in the semen of japanese quail.japanese quail (coturnix coturnix japonica) semen was collected at 10 a.m., 2 p.m., or 6 p.m. for four consecutive days during four consecutive weeks. all but the first day's collections were analyzed for sodium, potassium, chloride, carbon dioxide, h+ concentration, cell count, volume and total sperm number. a random sample was then collected for the analysis of amylase and another random sample was collected for the analysis of glutamic oxalacetic transaminase (got), creatine phosphokinase (cp ...19751187516
toxicity of dietary lead in japanese quail.the toxicity of dietary lead in japanese quail was investigated. the data indicated that dietary lead, in the form of lead acetate, was toxic to young quail at the level of 500 p.p.m. and this toxicity was evidenced by an inhibition of normal growth and by anemia. the anemic state in the lead toxic quail was more readily detected by reduced blood hemoglobin concentrations than by packed cell volumes. in addition, the data suggested that lead interfered with normal sexual development in the males ...19751187520
effect of turning and age of egg on hatchability in the pheasant, chukar, and japanese quail.eggs from pheasants, chukar partridge and japanese quail were stored 1 to 28 days in a cold box where temperature and relative humidity were maintained at approximately 16 degrees c. and 70 percent, respectively. half the eggs from each species was turned once daily from a 45 degree angle from the verticle to the opposite angle. turning eggs prior to incubation had no appreciable effect on hatchability of eggs stored up to 28 days. length of storage for eggs from the pheasant and quail was criti ...19751187527
[the amino acid sequence of the double-headed protein proteinase inhibitor from dog submandibular glands, i. structural homology to the pancreatic secretory trypsin inhibitors (author's transl)].the primary structure of the broad specificity proteinase inhibitor from dog submandibular glands was elucidated. the inhibitor consists of a single polypeptide chain of 117 amino acids which is folded into two domains (heads) connected by a peptide of three amino acid residues. both domains i and ii show a clear structural homology to each other as well as to the single-headed pancreatic secretory trypsin inhibitors (kazal type). the trypsin reactive site (-cys-pro-arg-leu-his-glx-pro-ile-cys-) ...19751213678
genetic differences in susceptibility to ulcerative enteritis in japanese quail.six lines of japanese quail were derived from a single foundation population. four of the lines were selected on the basis of family mean three week body weight, two were unselected and all lines were randomly mated. quail were housed in wire batteries during the brooding, rearing and laying periods. during the brooding period, hover temperature and floor space per chick were similar from line to line. for the rearing and laying periods, floor space per chick was approximately the same from line ...19751228728
the effect of fluoride supplementation on the strength of osteopenic bone.the strength of osteopenic bone from calcium deprived rats, quail and roosters was significantly reduced after fluoride supplementation. using a device which measures torque, femurs from rats fed low calcium diets with 100 parts per million fluoride added to the water supply fractured at lower torque values than those values observed for rats fed a low calcium diet without fluoride. fluoride administration and calcium restriction in adult roosters and young japanese quail also resulted in a redu ...19761261131
immunohistochemical localization of lh-producing cells in the adenohypophysis of the japanese quail, coturnix coturnix japonica.the cells that produce luteinizing hormone (lh) in the adenohypophysis of the japanese quail were identified immunohistochemically using anti-chicken lh serum and horseradish peroxidase-labeled goat anti-rabbit gamma globulin serum. the lh cells are localized in the caudal lobe of the pars distalis. they are elongate in shape shape and are polarized toward the sinusoids, especially in their active states. alterations in size of lh cells are directly related to changes in circulating lh levels as ...19761268921
plasma follicle-stimulating hormone during photoperiodically induced sexual maturation in male japanese quail.follicle-stimulating hormone (fsh) was measured in japanese quail using a heterologous radioimmunoassay, the specificty of which was confirmed by its cross-reactions with purified chicken fsh and luteinizing hormone (lh). plasma concentrations of fsh, lh and testosterone were determined in quail during the testicular growth and sexual maturation which follows their transfer from short to long daylengths. all three hormones could be detected in short-day birds but their concentrations were greatl ...19761270949
phylogeny of regulatory regions of vertebrate tyrosinase genes.highly homologous dna elements were found to be shared by the upstream regions of the mouse tyrosinase and tyrosinase related protein (trp-1) genes. several nuclear proteins were shown to bind to both of these upstream regions. shared homologous dna elements were also found in the 5' flanking sequences of japanese quail and snapping turtle tyrosinase genes. shared homologous nucleotide sequences were found to be scattered like an archipelago in the 5' upstream regions of mouse and human tyrosina ...19921292011
[the effect of culture media on bacteriocin production in various strains of bacteria].some staphylococcus and enterococcus strains were used to investigate the effect of culture medium on bacteriocin production. staphylococcus cohnii sc7, staphylococcus sp. ztj 151, s. saprophyticus ss 877, enterococcus faecium ef1 and e. faecalis efg2 were isolated from the rumen wall and contents of lambs, calves and fallow deer, enterococcus gallinarum eg10 and e. avium ea12 were isolated from the caecum of japanese quail. the tested bacteria belong to producers with a wide antimicrobial effec ...19921297243
osteoclasts from medullary bone of egg-laying japanese quail do not express the putative calcium 'receptor'.the present study reports the contrasting effects of extracellular calcium ([ca2+]e) elevation on cytosolic free calcium levels ([ca2+]i) of osteoclasts, freshly isolated either from medullary bone of the egg-laying japanese quail or from rat cortical bone. [ca2+]i was measured in single osteoclasts using the ca(2+)-sensitive fluorochrome, indo-1. we found that elevation of [ca2+]e failed to induce a rise of [ca2+]i in quail osteoclasts, whilst causing an elevation of [ca2+]i in rat osteoclasts. ...19921321642
outbreaks of egg drop syndrome due to eds-76 virus in quail (coturnix coturnix japonica).two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. the decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. in both the outbreaks haemagglutination inhibiting antibodies to eds-76 virus were detected. fluorescent viral antigen specific to eds-76 virus was also detected in the lining epithelial and glandular cells of the uterus.19921329304
host range of rous sarcoma virus pseudotype rsv(hprs-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated j.the host ranges of the rous sarcoma virus (rsv) pseudotype rsv(hprs-103) of a novel avian leukosis virus (alv), strain hprs-103, and representative rsv pseudotypes of subgroups a to f, have been determined in embryo fibroblasts from 12 avian species. domestic fowl, red jungle fowl, sonnerat's jungle fowl and turkey were susceptible to infection by rsv(hprs-103); ring-necked pheasant, japanese green pheasant, golden pheasant, japanese quail, guinea-fowl, peking duck, muscovy duck and goose were r ...19921331300
regulation of aromatase cytochrome p-450 (estrogen synthetase) transcripts in the quail brain by testosterone.the aromatase cytochrome p-450 (p-450arom) cdna, which was identified by homologies in the dna and in the deduced amino acid sequences with human p-450arom cdna, was isolated from a brain cdna library of japanese quail, demonstrating the presence of rna transcripts of p-450arom in the quail brain. to determine trace amounts of p-450arom mrna in the brain and to examine the effects of testosterone on its expression, a quantitative pcr method of rna transcripts was developed. brain total rna was s ...19921331667
a method to obtain avian germ-line chimaeras using isolated primordial germ cells.primordial germ cells (pgcs), collected from the blood of 2-day-old chick embryos, were concentrated by ficoll density centrifugation. the blood contained 0.048% pgcs and the concentrated fraction contained 3.9% pgcs in blood cells. the pgcs were picked up with a fine glass pipette, and one hundred were then injected into the terminal sinuses of 2-day-old japanese quail embryos (24 somites); bubbles were then inserted to prevent haemorrhage. the embryos were further incubated at 38 degrees c for ...19921339832
effects of alpha-methyl-para-tyrosine on monoamine levels in the japanese quail: sex differences and testosterone effects.experiments were performed to obtain more information on the regulation by steroids of catecholaminergic systems in the brain of japanese quail. dose-response and time-response experiments were first performed to determine optimal conditions for measuring turnover in the quail brain. the norepinephrine and dopamine turnover were then estimated in microdissected brain nuclei of birds that were either sexually mature or gonadectomized or gonadectomized and treated with testosterone. two major fact ...19921350749
characterization of a paired box- and homeobox-containing quail gene (pax-qnr) expressed in the neuroretina.the retina is an integral part of the central nervous system, and consists of two layers, the outer pigmented layer and the inner sensory layer or neuroretina (nr). the nr layer contains several strata of cells (glial and neuronal) derived from proliferating neuroectodermal precursors that differentiate after terminal mitosis. in vitro, nr cells can differentiate not only into neuronal and glial types, but also into pigment and lens cells. quail (coturnix coturnix japonica) nr cells (qnr) infect ...19921354346
comparative effects of the polychlorinated biphenyl mixture, aroclor 1242, on porphyrin and xenobiotic metabolism in kidney of japanese quail and rat.1. aroclor 1242 (500 mg/kg, p.o.) produced a marked increase in porphyrin content of quail kidney (1800-fold), and of rat kidney but to a lesser extent (6-fold). 2. delta-aminolevulinic acid synthetase activity was increased 12-fold in quail kidney but was unchanged in rat kidney following aroclor 1242 treatment. 3. uroporphyrinogen decarboxylase activity was significantly inhibited in quail kidney but not in rat kidney. 4. renal ethoxyresorufin o-deethylase activity was induced in rat and quail ...19921360369
induction of ovarian growth and ovulation by administration of a chicken gonadotrophin preparation to japanese quail kept under a short-day regimen.1. chronic administration of a glycoprotein fraction from chicken pituitary using an alzet osmotic pump at 12.5 micrograms/h for 2 weeks induced growth of ovarian follicles to a mature size in the ovary and deposition of yolk in sexually immature 6-week-old japanese quail females which were kept under 8l:16d short-day conditions. however, no ovulation was induced in these birds. 2. injection of 500 micrograms of the glycoprotein into other immature short-day females was performed following the a ...19921393679
[histology, immunocytology, histochemistry and muscle fiber innervation of the pectoralis major and supracoracoideus muscles of excalfactoria chinensis chinensis (l.)].the morphology of the pectoralis major muscle and its antagonist, the supracoracoideus muscle, of the chinese quail was studied in comparison with the japanese quail, in relation to behavioral characteristics. the actomyosin atpase reaction after alkaline and acid preincubation reveals two fibre types. the indirect immunofluorescence, using specific antibodies against 'slow' (twitch) myosin from the human vastus lateralis muscle, provokes a weak reaction. all fibres observed show focal, monoaxon ...19921414211
expression of japanese quail ovalbumin in saccharomyces cerevisiae.a cdna sequence coding for japanese quail ovalbumin was used for the construction of expression plasmid under the adh1 promoter of the yeast shuttle vector pvt101-u. the resulting recombinant expression vector pjk2 was used for the transformation of saccharomyces cerevisiae. expression of quail ovalbumin in yeast cells was demonstrated by western blotting followed by immunochemical detection.19921452097
influence of different photoperiods on development of gonad, cloacal gland and circulating thyroid hormones in male japanese quail coturnix coturnix japonica.one day old chicks of japanese quail were exposed to different photoperiods (ld, 8:16, 13.5:10.5, 16:8 and ll) and observations (testes weight, cloacal gland size, body weight and circulating thyroxine and triiodothyronine) were taken at the age of 3, 5, 7, 9 and 16 weeks. results indicate that immediate reproductive development occurred in birds exposed to long photoperiods (greater than 12 hr). growth under ld 8:16, was not apparent till 7th week and by 16 weeks, degree of gonadal development ...19921459646
cultivation of eimeria tenella in japanese quail embryos (coturnix coturnix japonica).complete development of eimeria tenella in japanese quail embryos was observed. sporozoites were inoculated into the allantoic cavity of 7-day-old japanese quail embryos (coturnix coturnix japonica), after which the infected embryos were incubated at 41 c. in the chorioallantoic membrane mature first generation schizonts, mature second generation schizonts, and gametes were detected at 48 hr postinoculation of sporozoites (pi), 84 hr pi, and 126 hr pi, respectively. mature gametes and zygotes we ...19921491294
[thr arteries of the male sex organs of japanese quail (coturnix coturnix japonica) and their development after hatching].a total of 83 male japanese quails of the following age groups were used for this study: 11, 18, 25, 32, 35, 37, 39, 41, 43 days, and 7, 9, 17 weeks after hatching. neoprene-latex, tensol-cement, and the china ink were injected via the aortic arch. in most of male japanese quails, the testes are supplied by the a. testicularis originated from the aorta descendens through a common trunk with the a. renalis cranialis. but in only 3 quails had this artery originated directly from the aorta descende ...19921497141
insects feeding on desert bighorn sheep, domestic rabbits, and japanese quail in the santa rosa mountains of southern california.desert bighorn sheep (ovis canadensis cremnobates), a domestic rabbit (oryctolagus cuniculus), and japanese quail (coturnix japonica) were used as bait animals to collect blood-feeding flies in an area of active blue-tongue and epizootic hemorrhagic disease virus transmission. precipitin tests were used to confirm the blood source where feasible. eight species of culicoides, members of the leptoconops kerteszi group, simulium spp., anopheles franciscanus, and stomoxys calcitrans were collected f ...19921512887
characterisation of newcastle disease viruses isolated in india.eleven newcastle disease virus (ndv) isolates obtained from outbreaks of disease in chickens (9) and japanese quail (2) in tamil nadu, india were characterised in pathogenicity tests, antigenically, using mouse monoclonal antibodies (mabs), and other established tests devised to distinguish between different strains. all 11 isolates were shown to be highly virulent for chickens. in indirect immunoperoxidase tests used to assess the ability of a panel of 28 mabs to bind to infected cell cultures, ...19921519416
immunocytochemical and scanning electron microscopic studies of atherosclerosis in japanese quail.the major cellular components of atherosclerotic lesions in several species have been shown to be smooth muscle cells (smc) and macrophages. many studies suggest the composition of a lesion varies depending on the stage of lesion development. for example, macrophages are believed to be involved in the initial events of fatty streak formation in many animals. this communication presents the first cellular study of quail atherosclerosis and demonstrates the alteration of cellular structure during ...19921539014
the superior cervical ganglia are not necessary for entrainment or persistence of the pineal melatonin rhythm in japanese quail.the avian pineal exhibits a daily rhythm in the synthesis and secretion of the hormone, melatonin, which is involved in maintaining temporal order within the circadian system of some species. the pineal is richly innervated by sympathetic nerves which originate in the superior cervical ganglia (scg) and, in the chicken, these nerves play a role in generating the melatonin rhythm. in the japanese quail, the pineal melatonin rhythm can be entrained by light perceived directly by the pineal or by l ...19921540830
changes in luteinizing hormone receptors in the granulosa and theca layers of the ovarian follicle during follicular maturation in the japanese quail.attempts were made to reveal changes in number and affinity of lh receptors in the avian ovary during the course of the final maturation of follicles. first, we characterized the hormone binding properties of lh receptors in ovarian follicles of the japanese quail by incubating a crude plasma membrane preparation of follicle layer with radioiodinated chicken lh prepared by a recently devised method. specific lh receptors with common binding properties were detected in both granulosa and theca la ...19921563613
renewal and proliferation of spermatogonia during spermatogenesis in the japanese quail, coturnix coturnix japonica.four different types of spermatogonia were identified in the seminiferous tubules of the japanese quail: a dark type a (ad), 2 pale a types (ap1 and ap2), and a type b. a model is proposed describing the process of spermatogonial development in the quail. the ad spermatogonia are considered to be the stem cells. each divides to produce a new ad spermatogonium and a ap1 spermatogonium during stage ix of the cycle of the seminiferous epithelium. an ap1 spermatogonium produces two ap2 spermatogonia ...19921571970
annual changes in levels of plasma lh and size of cloacal protrusion in japanese quail (coturnix coturnix japonica) housed in outdoor cages under natural conditions.japanese quail of the strain used in our laboratory do not show a complete decrease in levels of circulating luteinizing hormone (lh) concentrations and show no collapse of the testes following their transfer from long to short days under laboratory conditions. thus, merely manipulating photoperiods in the laboratory does not simulate an annual breeding cycle. to see whether an annual breeding cycle does exist in "our" quail under natural conditions, mature male birds were housed in individual c ...19921577244
termination of lh secretion in japanese quail due to high- and low-temperature cycles and short daily photoperiods.mature male japanese quail were transferred from 16l:8d (19 degrees) to one of the following combinations of daily light-dark and temperature cycles, 8l:16d (12 hr, 19 degrees:12 hr, 9 degrees), 12l:12d (12 hr, 19 degrees:12 hr, 9 degrees) and 12l:12d (16 hr, 19 degrees:8 hr, 9 degrees). the low temperature is for the middle of the dark period in each treatment. in the control groups, birds were transferred to the same photoperiodic conditions as the experimental groups, but without changes in a ...19921577245
immunocytochemical localization of androgen receptors in the male songbird and quail brain.the distribution of androgen receptors was studied in the brain of the japanese quail (coturnix japonica), the zebra finch (taeniopygia guttata), and the canary (serinus canaria) by immunocytochemistry with a polyclonal antibody (ar32) raised in rabbit against a synthetic peptide corresponding to a sequence located at the n-terminus of the androgen receptor molecule. in quail, androgen receptor-immunoreactive cells were observed in the nucleus intercollicularis and in various nuclei of the preop ...19921578004
studies on avian spinal cord chimeras. i. neurological and histological manifestations.spinal cord chimeras were produced by replacing a small fragment of neural tube of a 2-day-old white leghorn chicken embryo with a similar fragment from a japanese quail embryo. the embryo mortality was 61%, and 72% of hatched birds were 'cripples' and had to be sacrificed within 5 days after hatching. forty-nine chimeras, 10.9% of the total number of operated embryos, were alive for more than 3 weeks. for at least 17 days after hatching, all birds behaved like normal chicks, and the grey quail- ...19921582699
studies on avian spinal cord chimeras. ii. immune response of the chicken host to the graft of quail tissue.a previous study described clinical and pathological manifestations observed in 49 chimeras produced by replacing a small fragment of the neural tube of a chicken embryo by a similar fragment from a japanese quail embryo. predominant antibodies in sera of these birds were directed against xenogeneic antigens which are devoid of organ specificity and which are present in quail tissues but absent from chicken tissues. mixed agglutination test with quail cell monolayers detected such antibodies in ...19921582700
multilocus dna fingerprints in gallinaceous birds: general approach and problems.multilocus profiles were investigated in five different species of galliformes (ring-necked pheasant phasianus colchicus, indian peafowl pavo cristatus, japanese quail coturnix coturnix japonica, domestic chicken gallus gallus, and red grouse lagopus lagopus scoticus) using two human multilocus probes (33.6 and 33.15) in combination with each of four restriction enzymes (alui, ddei, haeiii or hinfi). all the species show a dna fingerprint-like pattern using at least one restriction enzyme in com ...19921612925
jun inhibits myogenic differentiation.myoblasts from skeletal muscle of chicken or japanese quail embryos were infected with avian sarcoma virus 17 (asv-17), a retrovirus carrying the jun oncogene. at high multiplicities of infection asv-17-induced morphologic transformation inhibited fusion of myoblasts into myotubes and stimulated extended replication. the expression of the muscle-specific proteins desmin, myosin and creatine phosphokinase was inhibited in asv-17-infected cultures. immunofluorescent staining detected strong expres ...19911656361
nucleotide sequence and evolution of coding and noncoding regions of a quail mitochondrial genome.segments of the japanese quail mitochondrial genome encompassing many trna and protein genes, the small and part of the large rrna genes, and the control region have been cloned and sequenced. analysis of the relative position of these genes confirmed that the trna(glu) and nd6 genes in galliform mitochondrial dna are located immediately adjacent to the control region of the molecule instead of between the cytochrome b and nd5 genes as in other vertebrates. japanese quail and chicken display ano ...19911706782
dotted white--a plumage color mutant in japanese quail (coturnix coturnix japonica).a plumage color mutant, dotted white (dtw), of japanese quail is described in this paper. the adult dtw mutant shows white plumage with a small colored spot on the head and/or back. the pigmented spots invariably appear on the dorsal region and never on the ventral region of the body. some birds lack colored spots and show pure white plumage. the chick of the dtw mutant shows cream yellow plumules similar to those of the white leghorn chick. colored spots also exist as in adults, and its colorat ...19921740163
effects of long term 2% fish oil supplements on tissue fatty acids, phospholipids, cholesterol, and arterial histology in japanese quail (coturnix coturnix japonica).the effects of 2% olive oil (oo) or fish oil (fo) (super epa500) dietary supplementation (9 months) on japanese "sea" quail was investigated. the animals were examined for tissue biochemical changes and possible blood vessel fatty deposition. the fatty acids of blood and tissue extracts from heart, liver and fat were analyzed by gas-chromatography/mass spectrometry. the ratio of arachidonic acid to eicosapentaenoic or docosahexaenoic acid was markedly decreased in fo treated animals compared to ...19911750803
[conformation of the third domain of turkey ovomucoid in solution. structural analysis by two-dimensional overhauser nuclear effect spectroscopy].the conformations of a polypeptide chain of turkey ovomucoid third domain and its modified form with split reactive site peptide bond leu-18--glu-19 have been determined by the literary two-dimensional nuclear overhauser effect spectroscopy data using an earlier suggested method. it has been found that the polypeptide domain backbone contains an alpha-helical fragment (residues 32-47), five segments having extended conformation (1-5, 11-17, 19-25, 29-31, 48-50) and beta-turn type 1 (26-29). segm ...19911753953
is the "mammalian" brown fat-specific mitochondrial uncoupling protein present in adipose tissues of birds?1. mitochondria were isolated from the furcular, subcutaneous, abdominal, nape and lateral adipose tissue depots of five species of bird (pheasant, japanese quail, pigeon, house sparrow and great tit) acclimatized to the northern winter. 2. mitochondrial proteins were separated by sds-polyacrylamide gel electrophoresis, blotted onto nitrocellulose membranes, and probed for the presence of the 32,000-33,000 mr uncoupling protein characteristic of "mammalian" brown adipose tissue, using an anti-(g ...19911756620
a comparative study of the structure of egg-white riboflavin binding protein from the domestic fowl and japanese quail.1. the riboflavin binding proteins from domestic fowl and japanese quail have been isolated and their structures compared by circular dichroism, fluorescence and peptide mapping. 2. the two proteins have similar secondary structures, but differ in their tertiary structures as reflected in the environments of aromatic amino acid side chains. 3. differences in amino acid sequence between the proteins are indicated by the digestion patterns obtained with thermolysin, chymotrypsin and v8 proteinase ...19911756622
studies on clinical signs and haematological alterations in pneumonic aspergillosis due to aspergillus flavus in japanese quail.intratracheal inoculation of 2-week old quail chicks with aspergillus flavus spores resulted in the development of clinical signs within 24 h of infection. these were characterized by dullness, depression, anorexia, accelerated breathing, gasping and prostration leading to death. these signs continued up to 7 days followed by considerable decrease in the intensity of the symptoms as well as number of birds showing clinical signs. mortality occurred primarily in the first week with a majority of ...19911779994
effect of feeding palmitic, oleic, and linoleic acids to japanese quail hens (coturnix coturnix japonica). 1. reproductive performance and tissue fatty acids.a study was conducted to evaluate the effects of diets containing 3% of either palmitic acid (diet pa), oleic acid (diet oa), or linoleic acid (diet la) on reproductive performance, fatty acid composition of egg yolk, plasma, and liver, and total plasma phosphorus of japanese quail. each diet was fed to 20 individually caged hens from 5 wk of age. a 24-wk production period started at 8 wk of age. fertile eggs for incubation were obtained by placing at random a male in the cage with the female fo ...19911784570
studies on the substrate specificity of neutral alpha-mannosidase purified from japanese quail oviduct by using sugar chains from glycoproteins.the substrate specificity of neutral alpha-mannosidase purified from japanese quail oviduct [oku, h., hase, s., & ikenaka, t. (1991) j. biochem. 110, 29-34] was analyzed by using 21 oligomannose-type sugar chains. the enzyme activated with co2+ hydrolyzed the man alpha 1-3 and man alpha 1-6 bonds from the non-reducing termini of man alpha 1-6(man alpha 1-3)man alpha 1-6(man alpha 1-3)man beta 1-4glcnac beta 1-4glcnac (m5a), but hardly hydrolyzed the man alpha 1-2 bonds of man9glcnac2. the hydrol ...19911794989
establishment and characterization of a thymidine kinase deficient avian fibroblast cell line derived from a japanese quail cell line, qt35.an avian thymidine kinase deficient (tk-) fibroblast cell line (qttk-) was established from a japanese quail cell line, qt35, and characterized the biological properties. qttk- could grow in the presence of 5-bromo-2'-deoxyuridine (budr, 100 micrograms/ml) and not in the growth medium with hypoxanthine-aminopterin-thymidine. compared to qt35 cells, the 3h-thymidine incorporation of the qttk- cells and the tk activity of the cell extract significantly decreased to 0.3% and 0.5%, respectively. in ...19911832032
parasitism of toxocara canis larvae in japanese quails by inoculation of the ascarid eggs.the distribution of t. canis larvae and pathological changes caused by them were studied in japanese quails orally inoculated with 1,500, 4,000 or 15,000 embryonated eggs. larvae were distributed mainly in the liver and, to lesser extent, in the muscles, brain, eyes and other organs. the number of larvae varied from 7 to 3,346, and from 1 to 288 in the liver and muscles (breast and legs), respectively. a small number of larvae were also recovered from the heart, gizzard, brain and eyes. in the g ...19911836373
protein structure and cdna nucleotide sequence of the japanese quail alpha a globin.the primary structure of the alpha polypeptide chain (alpha a) of the major component (qii) of japanese quail hemoglobin was determined by protein and cdna sequence analysis. the amino-acid sequences of all the soluble tryptic peptides were determined by the conventional protein sequencing technology. the sequence of the remaining portion, which contained an insoluble "core region", was determined through determination of the cdna nucleotide sequence. the cdna clones coding for the alpha a globi ...19911859627
effect of 12 hr temporal relation of serotonin and dopamine precursor drugs (5-htp and l-dopa) on photosexual responses of immature japanese quail.to study the interaction of photoperiod and circadian neurotransmitter activity, l-dopa l-dihydroxyphenylalanine, dopamine precursor) was administered daily, 12 hr after 5-htp (5-hydroxy tryptophan, serotonin precursor) in sexually immature japanese quail, raised under short photoperiod (ld8:16) since hatching. the 12 hr treatment of 5-htp and l-dopa was given under continuous condition of light for 11 days. after treatment the quail were transferred to intermediate day length (ld 13.5:10.5). th ...19911874551
avian lhrh during embryonic development: measurement by competitive elisa with a monoclonal antibody.a mouse monoclonal antibody directed against chicken gln8-luteinizing hormone-releasing hormone (clhrh-i) was developed and characterized. this antibody was used for the development of a competitive microtiter plate enzyme-linked immunosorbent assay for avian lhrh. the assay was validated for use with tissue and was used at a working range between 5 pg and 10 ng per sample. using this procedure, clhrh-i and ii were assayed in whole brain extracts of japanese quail embryos. samples were taken at ...19911879658
effects of first formant onset properties on voicing judgments result from processes not specific to humans.both first formant (f1) transition duration and f1 onset frequency have been proposed to be perceptually significant in categorization of voiced and voiceless syllable-initial stops. transition duration per se may not, however, explain the fact that, for longer transitions, longer f1 cutback is required in order to perceive a stop as voiceless. longer transitions result in lower f1 onsets at any duration of cutback greater than zero, and it is possible that the major effect of f1 is determined b ...19911880303
production of transgenic birds.the avian embryo presents a tremendous challenge for those interested in accessing and manipulating the avian germ line. by far the most successful method of gene transfer is by retrovirus vector. the efficacy of retrovirus vectors has been demonstrated by germ line insertion of replication-competent retroviruses as well as the insertion of replication-defective retrovirus vectors carrying bacterial marker genes. retroviral vectors have also been shown to be useful for the transfer and expressio ...19911915773
research note: effects of dietary fats on hepatic cholesterol synthesis in japanese quail.blood lipid concentrations and hepatic cholesterol synthesis were compared in japanese quail fed diets containing fats with different fatty acid profiles. the quail fed a diet containing tuna oil had the lowest blood cholesterol concentration; those fed beef drippings the highest, and those fed safflower oil or linseed oil had intermediate concentrations. rates of hepatic cholesterol synthesis mirrored the results for serum cholesterol concentration. serum triglyceride concentrations were lower ...19911924102
purification and characterization of neutral alpha-mannosidase that is activated by co2+ from japanese quail oviduct.an alpha-mannosidase was purified from the magnum section of japanese quail oviduct by ammonium sulfate precipitation, deae-sephacel chromatography, sephacryl s-300 chromatography, mannan-sepharose 4b chromatography, and hydroxyapatite chromatography. the purified alpha-mannosidase (referred to as neutral alpha-mannosidase) showed a single band on polyacrylamide gel with or without sodium dodecyl sulfate. its molecular weight was found to be 330,000 by gel chromatography. neutral alpha-mannosida ...19911939023
experimental infection of japanese quail with toxocara canis larvae through earthworms.the migration and distribution of toxocara canis larvae in the tissues of earthworms exposed to 5 x 10(5) infective eggs in soil, as well as the reinfectivity of the larvae in japanese quail fed with three earthworms, were studied. the average number of larvae recovered from an earthworm gradually declined from 9 +/- 3.21 to 4.5 +/- 2.00 at 4-16 days. no larvae were recovered at day 20. when compared between three portions of the earthworms' body, the middle part showed the greatest recovery in ...19911957494
japanese quail and human acid maltase deficiency: a comparative study.a morphological study was carried out on the skeletal muscle of a mutant japanese quail with acid maltase deficiency (amd). the affected quails began to experience difficulty in lifting their wings about 6 weeks after hatching. four weeks after hatching, before symptoms appeared, alpha-1, 4-glucosidase activity in skeletal muscle was decreased to less than 10% of the control level, and muscle fibers possessed many vacuoles containing periodic acid schiff (pas) positive material which was digeste ...19911957974
metabolism of narasin in chickens and japanese quail.thirty mature chicken hens and 60 mature japanese quail hens were used to compare pathways of narasin excretion. carbon-14-labeled narasin was injected into chickens (.7 microci) and quail (.113 microci) via cardiac puncture. blood, sampled at varying times thereafter, and eggs and excreta collected daily for 28 days, were analyzed for 14c. groups of six chickens and 12 quail were killed prior to [14c]narasin injection and on days 1, 7, 14, and 28 postinjection to obtain tissue samples for 14c a ...19912017406
hepatic 5'-deiodinase activity of japanese quail using reverse-t3 as substrate: assay validation, characterization, and developmental studies.using rt3 as substrate, an in vitro 5'd assay was validated for use with liver tissue from adult japanese quail, by defining conditions under which activity is proportional to enzyme (protein) concentration and is linear with incubation time. activity was measured as the release of 125i from labeled rt3. using validated assay conditions we found the following 5'd characteristics: maximal activity from 10 to 50 mm dithiothreitol (cofactor), an apparent km of 0.52 microm rt3, ph optimum of 7.6-8.5 ...19912022947
relative salience of species maternal calls in neonatal gallinaceous birds: a direct comparison of japanese quail (coturnix coturnix japonica) and domestic chickens (gallus gallus domesticus).differential-approach tendencies of individual incubator-hatched chickens and japanese quail were assayed using one exemplar of each of the species maternal calls in a simultaneous-choice paradigm. within 24 hr after hatching, the birds received several short periods of exposure to rotating mounts of an adult female chicken and quail, which emitted the species-appropriate call. all birds were then tested with calls alone over the following 2 to 4 days. the test stimuli were played from speakers ...19912032455
effects of surgical deafening and exposure to continuous darkness on cloacal gland size in scotorefractory japanese quail.adult male quail were transferred to short days (sd) and exposed simultaneously to conspecific vocalizations of males on long days (cvmld). cloacal glands did not regress in some males while in others their glands regressed then spontaneously recrudesced. of the birds that exhibited cloacal gland regression, 80% recrudesced by 8 weeks and all birds recrudesced by 25 weeks on sd. three experiments are reported. the first experiment analyzed the effects of transfer to sd. the remaining two experim ...19912045093
effects of selection on plasma thyroxine concentrations in japanese quail under thiouracil and protein stress.three experiments were conducted to measure changes in plasma thyroxine (t4) concentrations occurring in male and female japanese quail in response to treatment with dietary thiouracil (tu) and different cp levels prior to sexual maturity and to determine the influence of selection for growth under tu and protein stress on this response. selected and unselected lines of quail were fed diets containing .2% tu or two levels of cp (20 or 28%) or both from 0 to 4 wk of age. body weight and plasma t4 ...19912047354
maturation-promoting factor and p34cdc2 kinase during oocyte maturation of the japanese quail.maturation-promoting factor and a homolog of fission yeast cdc2+ gene product (p34cdc2) were investigated during the final 24 hr of maturation of quail oocytes. kinase activity of p34cdc2 in the oocyte germinal disk (gd) increased 15 times at maturation. two bands, at 32 and 34 kda, were detected in immature oocytes by immunoblotting of sds-page with anti-p34cdc2 monoclonal antibody. a new band, which is close to the 32-kda band but with a slightly faster mobility, appeared during maturation. no ...19912060707
purification and characterization of chymotrypsinogen from pancreas of japanese quail (coturnix coturnix japonica).1. a chymotrypsinogen from pancreas of japanese quail (coturnix coturnix japonica) was purified by acid extraction, salt fractionation and chromatographic separation on cm-cellulose and sephadex g-100, and gave a single protein band on sds-page. 2. quail chymotrypsinogen had a mol. wt of 26,100 calculated from amino acid composition data, an isoelectric point of 7.68, a km of 3.1 mm and k0 of 40.7 sec-1 for tyrosine ester substrate. 3. the activated chymotrypsinogen of quail had a maximum activi ...19902085958
interaction between eimeria uzura infection and aflatoxicosis in japanese quail (coturnix coturnix japonica).the clinical signs and gross lesions caused by eimeria uzura (10(5) oocysts) in japanese quail (coturnix coturnix japonica) exhibited little or no influence in the face of intercurrent dietary aflatoxicosis (1 p.p.m. of aflatoxin b1 from day 0 to 55). similarly, no significant differences in the mucosal morphology of the intestine were evident histologically between the two groups of japanese quail. the nervous signs of ataxia, leg weakness, incoordination of movement, torticollis and terminal o ...19902111599
ultradian and circadian compartmentalization of respiratory and metabolic exchanges in small laboratory vertebrates.carbon dioxide emission (vco2) taken as an index of respiratory and metabolic exchanges, was continuously recorded during 4-30 consecutive days in 100 quail, 87 chicks, 347 rats, 665 mice and 70 guinea-pigs which were under controlled environmental parameters. harmonic analysis, fast fourier transform, chi-square periodograms, peak and trough intervals were computed with vco2 values obtained with co2 concentrations sampled every 20 min on the co2 recordings. in ld 12:12 alternation, circadian rh ...19902128229
glycyl-l-glutamine stimulates the accumulation of a12 acetylcholinesterase but not of nicotinic acetylcholine receptors in quail embryonic myotubes by a cyclic amp-independent mechanism.myotubes prepared from the japanese quail embryo at 9 days gestation were cultivated in the presence of glycyl-l-glutamine (gly-gln, beta-endorphin c-terminal dipeptide) or glycyl-glutamic acid (gly-glu), and changes in the activity of acetylcholinesterase (ache) molecular forms and binding of 125i-alpha-bungarotoxin (alpha bgt) to cell surface nicotinic acetylcholine receptors were measured. the a12 oligomer was the major form of ache in the cultures. the activity of all molecular forms of the ...19902156012
vasopressor and depressor effects of native angiotensins and inhibition of these effects in the japanese quail.the amino acid sequence of angiotensin i (ang i) from the japanese quail, coturnix coturnix japonica, obtained from incubation of homologous plasma and kidney extract was determined as h-asp-arg-val-tyr-val-his-pro-phe-ser-leu-oh. a bolus, intravenous injection of native ang i or of ang ii induced an immediate vasodepressor response and a subsequent vasopressor response in quail which has been lightly anesthetized with urethane (0.75 g/kg). the values for ed50 for the vasopressor and depressor e ...19902191893
production of avian antibodies to three potyviruses in coturnix quail.avian antibodies against three potyviruses were produced in a small bird, coturnix quail (coturnix coturnix japonica temminck et schlegel), with 15-60 micrograms of purified virus preparations. intramuscular injections of immunogen with freund's incomplete or complete adjuvant into the birds did not result in higher titer of antibody compared to that of control birds given intravenous injections. quail egg yolk antibody was as useful as hen antibody for indirect-elisa and allowed virus to be det ...19902196273
high-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution.the three-dimensional structure of a 30-residue synthetic peptide containing the carboxy-terminal "zinc finger" motif of a human enhancer binding protein has been determined by two-dimensional nuclear magnetic resonance (2d nmr) spectroscopy and hybrid distance geometry-dynamical simulated annealing calculations. the structure determination is based on 487 approximate interproton distance and 63 torsion angle (phi, psi, and chi 1) restraints. a total of 40 simulated annealing structures were cal ...19902248949
behavior of rabbit dental tissues in heterospecific association with embryonic quail ectoderm.the behavior of dental tissues from the rabbit, oryctolagus cuniculus, in association with epithelium from the quail, coturnix coturnix japonica, has been examined. adult and embryo rabbits were employed in this study. dental papillae from teeth at the cap stage from rabbit embryos and dental pulp from adult rabbits were isolated surgically and recombined with skin ectoderm from 72-hour-old quail embryos. the recombined tissues were cultured for 48 hours on semi-solid medium and subsequently rem ...19902250161
distribution of aromatase in the brain of the japanese quail, ring dove, and zebra finch: an immunocytochemical study.an immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the brain of three avian species: the japanese quail, the ring dove, and the zebra finch. in quail and dove, immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the medial preoptic area, in the septal area above the anterior commissur ...19902262592
abdominal fat and testes weights in diverse genetic lines of japanese quail.six experiments were conducted to investigate abdominal fat levels in mature male and female japanese quail following selection for 4-wk body weight and to investigate the relationship between testes development and abdominal fat accumulation. the present study utilized p-, t-, and s-line quail selected for more than 75 generations for high 4-wk body weight and also lines divergently selected (16 or more generations) for high (h-sd, h-cd) and low (l-sd, l-cd) 4-wk body weight. adult males had fr ...19902263537
circulating estradiol and the activation of male and female copulatory behavior in japanese quail (coturnix japonica).previous experiments using systemic and preoptic area (poa) hormone treatments have shown that aromatization of testosterone (t) to estrogen (e) is essential for activation of male-typical copulatory behavior in castrated male japanese quail (coturnix japonica). two experiments were conducted to determine whether circulating estrogen levels characteristic of normal intact males are high enough to activate male-typical or female-typical copulatory behavior. in experiment 1, blood samples were dra ...19902307345
comparative angiotoxic responses of avian and rodent species in vivo: implications in atherogenesis.as the intrinsic susceptibility to atherosclerosis differs among several taxonomic groups, the present studies were conducted to compare the angiotoxic responses of atherosclerosis-susceptible (quail) and -resistant (rat) animals to allylamine, a selective cardiovascular toxin. japanese quail (125-150 g) and sprague-dawley rats (175-200 g) were gavaged daily for 1, 7, or 20 d with allylamine hcl (0.7, 7, and 70 mg/kg) or tap water. at the ultrastructural level, subchronic exposure of quail and r ...19902325152
the induction by testosterone of aromatase activity in the preoptic area and activation of copulatory behavior.a series of 4 experiments was designed to study the relationships between the activity of the aromatase (aa) in the preoptic area (poa) and the activation by testosterone (t) of copulatory behavior in gonadectomized male and female japanese quail. the induction of aa by t in the poa is dose- and time-dependent. levels of aa seen in sexually mature males are restored in castrated birds by a treatment with 20 to 40 mm silastic t capsules which produce physiological levels of steroid in the plasma. ...19902326347
experimental infection of mice with toxocara canis larvae obtained from japanese quails.the migration and distribution of toxocara canis larvae in the tissues of japanese quails, infected orally with 5 x 10(3) infective eggs, were studied, as well as the re-infectivity of these larvae in mice, inoculated with 50 larvae obtained from the liver of these quails. post-infection, the highest concentrations of larvae were found to be present in the liver of quails while only a few migrated to other tissues like lungs, heart, muscle and brain. the migration and distribution of the larvae ...19902332284
infection of japanese quail with toxocara canis larvae and establishment of patent infection in pups.migration and distribution of toxocara canis larvae in the tissues of japanese quail, infected orally with 5 x 10(3) infective eggs, and the establishment of patent infection from the quails to the definitive host, were studied. larval yield at necropsy from various tissues and organs of quail varied from 4.72 to 7.54% of the infective eggs inoculated within the period 1-60 days post-infection (pi). the total number of larvae recovered on different days showed a gradual increase. the percent ino ...19902353428
effect of diet and substrate on the in vitro measurement of cholesterol and fatty-acid synthesis in hepatic tissue of japanese quail.a comparison of acetate, glucose, mevalonate, or water as radioactive substrates for the hepatic synthesis of cholesterol, fatty acids, and glyceride-glycerol was made in japanese quail fed diets containing either beef fat or tuna oil. the quail fed a diet containing beef fat were fatter and had a significantly higher (p less than .01) concentration of serum cholesterol (5.6 mm per l) than that measured in the serum of quail given tuna oil (4.1 mm per l). both in vitro cholesterol and fatty-acid ...19902356181
immunocytochemical localization of aromatase in the brain.an immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the japanese quail brain. immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the sexually dimorphic medial preoptic nucleus, in the ventromedial nucleus of the hypothalamus and in the infundibulum. the positive material was localized ...19902357545
the effects of different iodide availabilities on thyroid function during development in japanese quail.japanese quail (coturnix japonica) were used to study the effects of different egg iodide (i) availabilities on thyroid function during development. low (less than 50 micrograms 1/kg feed in the maternal diet) and high (1200 micrograms 1/kg feed) i availability were compared to control levels (800 micrograms 1/kg feed), a standard supplementation for game bird feed. we measured thyroid gland content of i, triiodothyronine (t3) and thyroxine (t4), plasma concentrations of t3 and t4, hepatic 5' mo ...19902369822
induction of hepatic cytochrome p-450 mediated alkoxyresorufin o-dealkylase activities in different species by prototype p-450 inducers.the induction of cytochrome p-450-mediated alkoxyresorufin o-dealkylase activities by various xenobiotics was examined in liver from a variety of animal species in order to gain insights into the substrate specificities of the induced p-450s. we found that forms of cytochrome p-450 capable of mediating the o-dealkylation of the short-chain phenoxazone ethers methoxy-, ethoxy- and propoxyresorufin were highly induced by 3-methylcholanthrene-type inducers and by aroclor-1254 in all species tested, ...19902379261
isolation of campylobacter jejuni from the eggs and organs in experimentally infected laying japanese quails (coturnix coturnix japonica). 19902385046
gene organization of the peking duck mitochondrial genome.the gene organization of the peking duck mitochondrial (mt)dna has been deduced through heterologous hybridization using different cloned fragments of the chicken or japanese quail mitochondrial genome as probes. as in the chicken, and other gallinaceous birds, the peking duck mtdna displays a novel gene order which differs from that of other vertebrates by the unusual localization of the trna(glu) and nd6 genes next to the displacement (d) loop region of the molecule. the position of these gene ...19902390786
presence of retrovirus reverse transcriptase-related gene sequences in avian cells lacking endogenous avian leukosis viruses.using a molecularly cloned viral dna probe representing the entire avian sarcoma virus (asv) reverse transcriptase (pol) gene, we have detected related sequences in dna preparations from two avain species, ev- chickens and japanese quail, previously demonstrated to lack all endogenous avain leukosis viruses. nucleotide sequence homology was detected only when hybridization conditions, which allowed the formation of stable duplexes with as much as 30% base mismatch, were used. no sequence homolog ...19852410912
steroidogenesis in ovarian cells of the japanese quail (coturnix coturnix japonica).the influence of follicular maturation on steroidogenesis and steroid metabolism by isolated japanese quail granulosa and theca cells was examined. when stimulated with lh, granulosa cells of the largest follicle (f1) responded with a sixfold increase over unstimulated progesterone levels, whereas progesterone production in cells of f3 less than doubled even when maximally stimulated. forskolin stimulated progesterone synthesis in both f1 and f3 granulosa cells, but its effect was less pronounce ...19852416631
isolation and partial characterization of japanese quail oviduct mrnas.the total mrna was prepared from laying quail oviduct and, after denaturation with dimethyl sulphoxide and glyoxal, characterized by agarose gel electrophoresis. the electrophoresis showed that the preparation contains physically homogeneous molecules of mrnas, which migrate as discrete bands. the molecular weights of four major oviduct mrnas coding for conalbumin, ovalbumin, ovomucoid, and lysozyme were determined. the biological activity of mrnas was assayed in a cell-free translation system d ...19862428676
molecular cloning and nucleotide sequence analysis of the putative cdna for the precursor molecule of the chicken lh-beta subunit.a cdna expression library was constructed from poly(a)+ rna of broiler chicken adenohypophyses using lambda gt11 as a vector. after screening with a rabbit antiserum against chicken lh, a cdna clone (l12) containing a 436 bp insert was obtained. using a subclone of l12 in puc19 (pl12) as the hybridization probe, another cdna clone (lf127) with a 533 bp insert was isolated. the lf127 contained the full-length cdna encoding the putative chicken lh-beta subunit precursor molecule. hybridization of ...19892476156
demethylation of the gene expressing a yolk protein precursor in quail.japanese quail produce more egg-yolk protein per gram of body weight than chickens do. because cytosine phosphoguanine (cpg) methylation has been reported to correlate inversely with gene activity, the demethylation of cytosine residues in the quail vitellogenin 2 (vg2) gene was assessed as a possible contributing factor. vitellogenin genes are normally transcribed in the liver of female japanese quail that are sexually mature. changes in the methylation pattern of ccgg sites of the quail vg2 ge ...19892482967
mature 98,000-dalton acid alpha-glucosidase is deficient in japanese quails with acid maltase deficiency.we compared acid alpha-glucosidase of acid maltase-deficient japanese quails, an animal model of human late-onset glycogenosis type ii, with that of normal controls. antibody produced in a rabbit against acid alpha-glucosidase purified from chicken pectoral muscle cross-reacted with that of japanese quails. the presence of a 110k and 98k form of acid alpha-glucosidase was confirmed in normal controls by immunoblotting. however the 98k form was absent in the affected quails. subcellular distribut ...19892506448
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