| protection against chlamydia psittaci in mice conferred by lyt-2+ t cells. | a murine model was used to study the respective roles of l3t4+ and lyt-2+ t cells in protection against chlamydia psittaci. donor mice were intravenously (i.v.) infected with 1 x 10(5) plaque-forming units (pfu) per mice of live c. psittaci. one month after inoculation, splenic cells from donors were transferred into syngenic recipients (5 x 10(7) cells/mouse). as measured by splenic colonization on day 6 after i.v. challenge (1 x 10(5) pfu/mouse), transfer with primed (untreated) cells conferre ... | 1992 | 1427980 |
| acquisition and synthesis of folates by obligate intracellular bacteria of the genus chlamydia. | we undertook studies focused on folate acquisition by chlamydia trachomatis l2, chlamydia psittaci 6bc, and c. psittaci francis. results from in situ studies, using wild-type host cells, confirmed that c. trachomatis l2 and c. psittaci 6bc are sensitive to sulfonamides whereas c. psittaci francis is resistant. in addition c. trachomatis l2 and c. psittaci francis were inhibited by methotrexate in situ whereas c. psittaci 6bc was not. in contrast to c. trachomatis, neither c. psittaci strain was ... | 1992 | 1430206 |
| [study on the bacteriological examination of sputum and bronchoscopy specimens from 31 cases with pneumonia due to chlamydia psittaci]. | we carried out the bacteriological examination of sputum and bronchoscopy specimens from 31 cases with pneumonia due to c. psittaci. the results obtained were as follows: 1. the positive culture of sputum and bronchoscopy specimens were 38.7% (12/31). 2. the organisms detected from them were 13 strains of gram-negative bacilli, 2 of gram-positive cocci and one gram-positive bacillus. 3. significant differences were observed in the white blood cell count between the cases of positive culture and ... | 1992 | 1431354 |
| culture of sheep chlamydia in a sheep fibroblast cell culture. | abortion and enteric isolates of chlamydia psittaci from sheep differed in their growth in a fibroblastic cell culture derived from the small intestine of a lamb. twenty abortion isolates, each from a different farm, produced large inclusions which could be passaged several times whereas 10 enteric isolates each from different farms (but from some of the farms of origin of the abortion isolates) produced sparse inclusions which could not be passaged. this appears to be a rapid method of distingu ... | 1992 | 1439220 |
| [epidemiology of micro-organisms responsible for community-acquired pneumonia]. | pneumonias occupy a prominent situation among lower respiratory tract infections where they are remarkable for their potential mortality and for our relative knowledge of the responsible micro-organisms. analysis and synthesis of each series published must answer several questions, such as: what are the lung diseases considered? which investigations have been performed? which criteria of imputability have been used? in which patients has the study been carried out? in which place, which period a ... | 1992 | 1439460 |
| enzyme electrophoretic polymorphism differentiates invasive from non-invasive chlamydia psittaci ruminant isolates. | a group of 24 chlamydia psittaci strains isolated from ruminants, belonging to serotype 1 and previously classified as invasive in a mouse model of virulence, was compared to a group of 10 non-invasive strains belonging to serotype 2 by using determination of glucose-6-phosphate and l-malate dehydrogenase zymotypes resulting of the infection of cells by these strains. the serotype 1 or invasive isolates represent a homogeneous group by sharing a unique zymotype which was not observed in the non- ... | 1992 | 1448629 |
| establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the ideia chlamydia kit and dna probe by using the purified elementary bodies. | to evaluate the sensitivity of commercially available test kits for detection of chlamydiae, we established a method of purifying chlamydia trachomatis and chlamydia pneumoniae elementary bodies (ebs). we then subjected the purified ebs, together with the purified ebs of chlamydia psittaci, to the ideia chlamydia (ideia) and dna probe test kits to determine the eb numbers at the detection limits. the sensitivities of the test kits were thus compared. the results can be summarized as follows. (i) ... | 1992 | 1452662 |
| comparison of nine antigen detection kits for diagnosis of urogenital infections due to chlamydia psittaci in koalas. | chlamydia psittaci is the major cause of infectious disease in the koala (phascolarctos cinereus). it causes four disease syndromes in the koala, namely, conjunctivitis, rhinitis, cystitis, and infertility (females only). diagnosis of chlamydial infections in koalas relies primarily on isolation of the organism in cell culture. serology has generally not been useful, and little use has previously been made of the commercially available antigen detection kits. we examined the sensitivity, specifi ... | 1992 | 1452703 |
| isolation of chlamydia psittaci from pleural effusion in a dog. | | 1992 | 1457552 |
| preparation and use of a monoclonal antibody to detect chlamydia psittaci antigen in paraffin-embedded tissue sections. | a murine monoclonal antibody prepared against an ovine abortion isolate of chlamydia psittaci (a22/teramo) revealed specific binding to a 57 kda chlamydial antigen in immunoblotting studies. the monoclonal antibody was able to detect intracytoplasmic chlamydial inclusions and scattered elementary bodies in infected mccoy cell culture, and on formalin-fixed paraffin-embedded tissue sections both from experimentally infected mice and from fetal membranes of cases of ovine enzootic abortion. | 1992 | 1465516 |
| [the signal transduction mechanism responsible for interferon-gamma-inducible indoleamine 2,3-dioxygenase (ido) gene expression in t98g cells]. | the interferon (ifn)-gamma-induced indoleamine 2,3-dioxygenase (ido) is implicated in the inhibition of intracellular pathogens, e.g. chlamydia psittaci and toxoplasma gondii. the intracellular signaling molecules responsible for the induction of ido gene expression were investigated by the quantitative polymerase chain reaction. the gene expression was inhibited by a tyrosine kinase inhibitor, genistein. being consistent with this, ifn-gamma induced increased tyrosine phosphorylation and this w ... | 1992 | 1469778 |
| specificity of the microimmunofluorescence assay for the serodiagnosis of chlamydia pneumoniae infections. | chlamydia pneumoniae infections are mostly confirmed using an indirect microimmunofluorescence test for which potential cross-reactions between antigens from different chlamydial species are not well documented. using this assay, 928 sera (507 subjects) submitted for chlamydia pneumoniae serology were tested for specific igm and igg to this bacteria using the tw-183 antigen. igm and igg reactivities to chlamydia trachomatis serotypes c, d, e, and l2 and chlamydia psittaci strain 6bc antigens wer ... | 1992 | 1477792 |
| [bilateral pneumonia caused by chlamydia psittaci. description of a clinical case]. | a mortal case of atypical pneumonia due to chlamydia psittaci with acute respiratory distress, septic shock and multiple organ failure is described. infection has been caused by an ill parrot imported clandestinely. antibody titration with the immunofluorescence technique allowed diagnosis. | 1992 | 1508365 |
| ophthalmologic examination of the normal eye of the koala. | the ophthalmic examination of the koala (phascolarctos cinereus) was undertaken to describe the normal in vivo structure. twenty-two of 28 koalas examined were found to have normal eyes. slit-lamp examination revealed unusual lamellae in the cornea, and mean keratometric readings were 59.3 +/- 3 diopters, corneal endothelial cell area of 381.4 microns2 +/- with a standard deviation of 46.8 microns2. no particular features were seen which would explain the susceptibility of this animal to ocular ... | 1992 | 1512874 |
| serologic survey for selected arboviruses and other potential pathogens in wildlife from mexico. | during 1988 and 1989, a serologic survey of wildlife was conducted in northeastern mexico to determine the presence, prevalence, and distribution of arboviruses and other selected disease agents. eighty mammal specimens were tested. antibodies to vesicular stomatitis-indiana, venezuelan equine encephalitis-mena ii, rio grande virus, and vesicular stomatitis-new jersey were detected predominantly in small mammals. deer and mouflon (ovis musimon) had antibodies to bluetongue and epizootic hemorrha ... | 1992 | 1512876 |
| monoclonal antibody based capture elisa/elifa for the detection of chlamydia psittaci in veterinary clinical specimens. | a capture elisa/elifa system based on monoclonal capture and biotinylated monoclonal detection antibody with specificity for an epitope on chlamydial lipopolysaccharide (lps) is described. the assay is fast, specific and detects a minimum dose of approximately 2000 chlamydial particles (c. psittaci). unlike other commercially available test kits, it comprises a proteinase k treatment to exclude non-specific binding of antibodies to fc receptors. in contrast to the sophisticated and cumbersome is ... | 1992 | 1520968 |
| pneumonia due to legionella bozemanii and chlamydia psittaci/twar following renal transplantation. | chlamydia and legionella are recognized causes of atypical pneumonia. a case of pneumonia due to chlamydia psittaci/twar and legionella bozemanii following renal transplantation is described. legionella bozemanii infection was diagnosed by a rise in antibodies and by isolation of the organism from bronchoscopy specimens. it is unusual to find pneumonia caused concomitantly by two such agents. this case, despite the fatal outcome, emphasises the necessity for a comprehensive approach to the diagn ... | 1992 | 1522325 |
| ovine chlamydiosis in an abattoir worker. | the strain of chlamydia psittaci causing enzootic abortion in ewes (the eae strain) may cause serious infection in pregnant women, often resulting in hepatic and renal dysfunction, disseminated intravascular coagulation and fetal loss. the first case of such an infection in an abattoir worker is described and the possibility of human-to-human transmission considered. direct handling of sheep or their products of conception can usually be established but this is not always so. there is much still ... | 1992 | 1522332 |
| coxiella burneti endocarditis in a patient with positive chlamydial serology. | a 41-year-old man who habitually slept in a car park presented with a culture-negative endocarditis. serological tests indicated infection with both coxiella burneti and chlamydia psittaci. he was treated with doxycycline and clindamycin and required aortic valve replacement. culture of the excised value for both agents was negative but electron microscopy was suggestive of coxiella endocarditis. | 1992 | 1522334 |
| the differentiation of chlamydia species by antigen detection in sputum specimens from patients with community-acquired acute respiratory infections. | an amplified enzyme immunoassay (ideia iii: dako diagnostics ltd) for detecting genus-specific chlamydia antigen was evaluated prospectively on 286 respiratory specimens from 275 patients presenting with community-acquired pneumonia or persistent chest infection. nineteen patients had evidence of recent chlamydial infection, having two or more positive sputum or serological markers. sputa from two other patients were elisa-positive in the absence of other positive criteria and were regarded as f ... | 1992 | 1522342 |
| a comparison of the seroepidemiology of chlamydial infection in pigeon fanciers and farmers in the u.k. | a detailed comparative seroepidemiological study of antibody responses was performed in 271 pigeon fanciers and 100 farmers. overall 73% of pigeon fanciers had igg antibodies at a titre greater than or equal to 16 to chlamydia pneumoniae, 39% to chlamydia psittaci, and 6.6% to chlamydia trachomatis. the prevalence of chlamydial antibodies was significantly lower in the farmers at 47% for c. pneumoniae, 6% for c. psittaci, and 2% for c. trachomatis. both populations were exposed to complex microb ... | 1992 | 1522344 |
| detection of chlamydia pneumoniae by polymerase chain reaction. | while criteria for serodiagnosis of chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. to increase detection of this organism, c. pneumoniae-specific sequences were identified to permit amplification of c. pneumoniae by polymerase chain reaction (pcr). a cloned c. pneumoniae 474-bp psti fragment was shown by dot blot and southern hybridization to differentiate c. pneumoniae from the other chlamydia spp., react with all c. pneumoniae isolates tested ... | 1992 | 1537913 |
| l-arginine-dependent reactive nitrogen intermediates and the antimicrobial effect of activated human mononuclear phagocytes. | the l-arginine-dependent generation of reactive nitrogen intermediates (rni) has been identified as a key intracellular antimicrobial mechanism of activated mouse macrophages. to determine the role of this mechanism in the activity of human mononuclear phagocytes, monocyte-derived macrophages activated in vitro by interferon (ifn)-gamma and monocytes from patients receiving ifn-gamma as therapy were treated with ng-monomethyl-l-arginine (nma) or arginase. neither competitive inhibition of l-argi ... | 1992 | 1538156 |
| interspecies structural diversity among chlamydial genes encoding histone h1. | recently, a eukaryotic histone h1-like protein has been detected in chlamydia trachomatis serovar l2 [hackstadt et al., proc. natl. acad. sci. usa 88 (1991) 3937-3941; tao et al., j. bacteriol. 173 (1991) 2818-2822]. we have cloned the corresponding gene from c. trachomatis serovar j and the chlamydia psittaci strain mn. sequencing demonstrated absolute gene identity between the two c. trachomatis serovars l2 and j, but divergence in the c. psittaci strain mn. these differences resulted in alter ... | 1992 | 1551591 |
| brief report: chlamydia psittaci endocarditis diagnosed by blood culture. | | 1992 | 1557094 |
| antigenic and morphological differentiation of placental and intestinal isolates of chlamydia psittaci of ovine origin. | ewe placental and lamb intestinal isolates of chlamydia psittaci recovered from flocks affected with ovine enzootic abortion were examined by inclusion morphology, indirect immunofluorescence (iif) and immunoblot analysis. chlamydiae recovered from the faeces of sheep from two flocks free of clinical disease were also examined. in cell culture ovine abortion (oa) and intestinal isolates were distinguishable by inclusion development and morphology. similarly, in two-way iif tests with one week mo ... | 1992 | 1557895 |
| [multicausal infectious respiratory tract disease of young fattening turkeys]. | the outbreak of the disease occurred in a large multiple-age farm with about 50,000 meat turkeys, where groups of 6-8000 one-day-old birds were stalled up every 14 days. all the turkey poults housed were affected mostly in the 1.-3. week of the life. the respiratory disease spread rapidly within the flocks and were characterised clinically by inclination of huddle, ruffled feathers, anorexia, stunted growth, swelling of the infraorbital sinus and nasal discharge. the clinical apparent disease la ... | 1992 | 1559465 |
| pathogenesis of endometritis and salpingitis in a guinea pig model of chlamydial genital infection. | the development of tubal obstruction and subsequent infertility is a major sequelum of upper genital tract infection with chlamydia trachomatis; however, little is known about the pathogenesis of the infection. in this investigation, the authors present a detailed study of the progression of ascending chlamydial infection in female guinea pigs resulting from intravaginal inoculation of the chlamydia psittaci agent of guinea pig inclusion conjunctivitis (gpic). isolation of chlamydiae from differ ... | 1992 | 1562052 |
| effects of interferons beta or gamma on neopterin biosynthesis and tryptophan degradation by human alveolar macrophages in vitro: synergy with lipopolysaccharide. | interferons can induce neopterin biosynthesis and tryptophan degradation in monocytic cells. indoleamine 2,3-dioxygenase (ido), an inducible cellular enzyme, metabolizes tryptophan to n-formyl-l-kynurenine. tryptophan degradation has been linked to interferon-mediated inhibition of replication by intracellular pathogens and inhibition of cancer cell proliferation. we evaluated the ability of the recombinant human interferons beta ser and gamma to stimulate neopterin production and tryptophan deg ... | 1992 | 1591013 |
| [rational means of the determination of costs and etiological diagnosis of community-acquired pneumonia]. | the cost and effectiveness of examinations (sputum staining and culturing, antitest determination for influenza a and b, rsv, adenovirus, chlamydia psittaci and pneumoniae, coxiella burnetii, mycoplasma pneumoniae and legionella pneumophila, and determination for streptococcus pneumoniae antigen) performed to explore the aetiology of community-acquired pneumonia in the case of 258 hospitalised patients were analysed. the aetiology could be determined in 44.2% of the cases. on the basis of prevai ... | 1992 | 1608606 |
| chlamydia psittaci infection in horses: results of a prevalence survey and experimental challenge. | nasal and conjunctival swabs were obtained from 300 horses and chlamydia psittaci was isolated from 15 of them (5 per cent). eleven nasal swabs and six conjunctival swabs were positive on culture, but there was no association between the isolation of the organism and the presence of clinical ocular or respiratory disease. six ponies were challenged with an equine isolate of c psittaci into the eye, nasal cavity or bronchial tree. the organism could be isolated from nasal and conjunctival swabs t ... | 1992 | 1609475 |
| evaluation of a commercial solid-phase enzyme immunoassay for the detection of ovine chlamydia psittaci. | | 1992 | 1616986 |
| diagnosis of avian chlamydiosis: specificity of the modified giménez staining on smears and comparison of the sensitivity of isolation in eggs and three different cell cultures. | for the diagnosis of chlamydiosis in dead and live birds different methods were compared for their sensitivity and specificity. the specificity of the modified giménez staining and the direct immunofluorescence (dif) test for direct demonstration of chlamydia psittaci in organ, cloacal and/or conjunctival smears was examined. the sensitivity of the isolation of chlamydia psittaci in 6 days embryonated specific pathogen free (spf) chicken eggs, buffalo green monkey (bgm) cell line, mccoy cell lin ... | 1992 | 1621472 |
| acute glomerulonephritis following infection with chlamydia psittaci. | | 1992 | 1621687 |
| [latent chlamydia infections as the cause of health disorders in swine, cattle and sheep breeders in czechoslovakia]. | for a twelve year period the health condition of a large group of pig-, cattle- and sheep-breeder was controlled and at the same time an inquiry was carried out why these people suffer from high occurrence of specific health problems which among the rest of the population appear in smaller extent. the performed investigation shows that the primary cause of the specific problems is a latent infection caused by the chlamydia microorganism, namely by the chlamydia trachomatis group, that can be tra ... | 1992 | 1622377 |
| [results of studies with aborted cattle fetuses]. | the laboratory of the animal health service of south holland investigated 2410 cases of spontaneous abortion (twins (112) were considered one case). seventy-eight afterbirths without foetuses were also examined. the aim of the study was not only to find the cause of abortion but also to see whether there was a relation between the results of the different bacteriological, virological and histological tests used. the presence of igg in the blood of foetuses older than 5 months was also determined ... | 1992 | 1626316 |
| restriction endonuclease analysis of dna from ruminant chlamydia psittaci and its relation to mouse virulence. | dna from 20 pathogenic or non-pathogenic ruminant strains of chlamydia psittaci was compared by restriction endonuclease analysis. the strains could be easily differentiated according to their invasiveness for mouse, whatever their pathological origin. dna patterns of invasive strains were similar, whereas those of non-invasive strains were distributed in two groups. | 1992 | 1626375 |
| [coxiella burnetii and chlamydia psittaci infection in dogs]. | the prevalence of coxiella burnetii and chlamydia psittaci antibodies was investigated in 530 dog specimens divided into six groups, i. e. a = private watch dogs, b1 = service dogs from bratislava, b2 = service dogs from other localities of slovakia and moravia, c = watch dogs from farms, i = household dogs, t = stray dogs. the dogs demonstrated the higher seropositivity to c. burnetii (11.7%) than to ch. psittaci (5.5%). the highest percentage of antibodies to c. burnetii was found in stray dog ... | 1992 | 1641947 |
| prevalence of antibodies to five selected zoonosis agents in monkeys. | the prevalence of antibodies against 5 zoonosis agents was determined in serum samples of 443 breeding monkeys. of the monkeys, 296 were bred or kept for a long time at r institute, and the remaining 147 were newly imported from the philippines and kept at s institute for quarantine. antibodies to simian virus 40 were highly prevalent at 89.1% among monkeys of r institute, whereas no antibody could be detected in those of s institute. antibodies to chlamydia psittaci and yersinia pseudotuberculo ... | 1991 | 1657210 |
| comparison of three-day and five-day courses of azithromycin in the treatment of atypical pneumonia. | this open, randomised clinical study, conducted from june 1988 to december 1989, included 84 patients with clinical and radiological findings of atypical pneumonia. all patients were treated with a total dose of 1.5 g azithromycin, a new azalide antibiotic. in group i, azithromycin was administered for three days (500 mg once daily). in group ii, azithromycin was administered for five days (250 mg b.i.d. on day 1, followed by 250 mg once daily on days 2 to 5). causative pathogens were identified ... | 1991 | 1662637 |
| morphological studies of the association of mitochondria with chlamydial inclusions and the fusion of chlamydial inclusions. | the association of mitochondria with the inclusions of five strains of chlamydia psittaci, two strains of c. trachomatis and one strain of c. pneumoniae was examined by electron microscopy. the results demonstrated that mitochondrial association occurred only with the c. psittaci inclusions, but not with the c. trachomatis and c. pneumoniae inclusions. multiple inclusions formed by infection with a single strain could fuse with each other during their expansion, but fusion never occurred between ... | 1991 | 1666645 |
| typing chlamydia trachomatis by detection of restriction fragment length polymorphism in the gene encoding the major outer membrane protein. | a method that avoids culture was devised to determine serovars of chlamydia trachomatis. polymerase chain reaction was used first to amplify a part of the chlamydial genome that included the leader sequence and all four variable domains of the major outer membrane protein (momp) of the 15 serovars of c. trachomatis. the amplified dna was then digested simultaneously with restriction endonucleases alui and mspi and the resulting fragments separated on 10% polyacrylamide gels. after silver stainin ... | 1991 | 1673464 |
| restriction pattern of the major outer-membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of chlamydia psittaci. | thirty-six ruminant isolates of chlamydia psittaci, previously classified as invasive or non-invasive in a mouse model of virulence, were compared by analysing alui restriction patterns of the major outer-membrane protein (momp) gene after dna amplification by the polymerase chain reaction. the 24 invasive isolates, although from various origins, all belonged to serotype 1 and represented a strictly homogeneous group sharing a specific momp-gene restriction pattern that was not observed in the n ... | 1991 | 1686054 |
| a simple staining method for the identification of chlamydial elementary bodies in the fetal membranes of sheep affected by ovine enzootic abortion. | a dark-ground methylene blue (dgmb) staining method was used to demonstrate chlamydial elementary bodies in fetal membranes of sheep affected by chlamydia psittaci. before evaluation on material from clinically affected animals, the dgmb method was compared with modified ziehl-neelsen (mzn) and dark-ground giemsa (dgg) staining methods for its ability to demonstrate chlamydial elementary bodies in hens' eggs which had been experimentally infected with c. psittaci. dgmb was more specific in its s ... | 1990 | 1689523 |
| antibody response to epitopes of chlamydial major outer membrane proteins on infectious elementary bodies and of the reduced polyacrylamide gel electrophoresis-separated form. | approximately 60% of the outer membrane of chlamydial elementary bodies (ebs) consists of the major outer membrane protein (momp) that has structural and metabolic functions. the antigenic properties of momps from mammalian strains of serovars 1 and 2 and an avian strain of chlamydia psittaci were analyzed. polyclonal-monospecific antisera (pmas), one monoclonal antibody (mab), and polyclonal antisera (pas) were produced against reduced polyacrylamide gel electrophoresis-separated momps and agai ... | 1990 | 1691145 |
| [effects of deae-dextran, centrifugation, cycloheximide and their combination on infection and growth of chlamydia psittaci bird isolates]. | in order to establish a stable and uniform cultural method in a cell line hala 229, we studied the effect of deae-dextran, centrifugation, cycloheximide and their combination on infectivity and progeny yields of chlamydia psittaci isolated mainly from birds. of 11 strains (10 avian and one human origin ito strain), 9 showed maximal inclusion formation when host cells were treated with a combination of dextran and cycloheximide. of the other two strains, one showed maximum inclusion formation wit ... | 1990 | 1692335 |
| developmental cycle-specific host-free rna synthesis in chlamydia spp. | the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ... | 1990 | 1698176 |
| immunologic characterization of a cloned fragment containing the species-specific epitope from the major outer membrane protein of chlamydia trachomatis. | a 183-bp fragment encoding variable domain iv (vd iv) of chlamydia trachomatis serovar b major outer membrane protein (momp) (amino acids 273 to 333) and containing the species-specific epitope was cloned and expressed in escherichia coli as a fusion protein with schistosoma japonicum glutathione s-transferase (gst-vd iv). the fusion protein was affinity purified under nondenaturing conditions and used to immunize rabbits. antisera were characterized by microimmunofluorescence, immunoblot, dot b ... | 1990 | 1701415 |
| characterization of circulating antibodies against chlamydia psittaci in turkeys. | plasma and joint fluids from turkeys experimentally inoculated with chlamydia psittaci strain tt3 were evaluated by immunoblotting to identify antibodies elicited by chlamydial antigens during the course of infection. protein antigens from elementary bodies of tt3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred electrophoretically to nitrocellulose before being probed with plasma or synovial fluid from tt3-inoculated birds. the major outer-membrane pro ... | 1990 | 1704213 |
| partial characterization of an 89-kda highly immunoreactive protein from chlamydia psittaci a/22 causing ovine abortion. | an 89-kda immunogen from chlamydia psittaci a/22 causing ovine abortion was partially characterized. the 89-kda protein, localized on the outer membrane complex of chlamydiae, was synthesized relatively early in the developmental cycle. the protein contained cysteine but was not extensively cross-linked by disulfide bonds. treatment with proteases apparently did not cleave the protein. the infectivity of strain a/22 was partially (60%) reduced by treatment of chlamydial elementary bodies with mo ... | 1991 | 1714846 |
| implications of interferon-induced tryptophan catabolism in cancer, auto-immune diseases and aids. | tryptophan (trp) is an indispensable amino acid required for biosynthesis of proteins, serotonin and niacin. indoleamine 2,3-dioxygenase (ido) is induced by infections, viruses, lipopolysaccharides, or interferons (ifns) and this results in significant catabolism of trp along the kynurenine (kyn) pathway. intracellular growth of toxoplasma gondii and chlamydia psittaci in human fibroblasts in vitro is inhibited by ifn-gamma and this inhibition is negated by extra trp in the medium. similarly, gr ... | 1991 | 1722946 |
| [monoclonal antibodies to chlamydia psittaci: their isolation, characteristics and use]. | a panel of 22 hybridomas producing monoclonal antibodies (mcab) to c. psittaci was obtained. 15 hybridomas produced igg1 antibodies, 4 hybridomas produced igm antibodies and 3 hybridomas produced igg2b, igg3 or iga antibodies. igg1 antibodies and 2 igm antibodies did not bind complement in the complement fixation test. all mcab were reactive in the enzyme immunoassay and the indirect immunofluorescence test and did not precipitate specific antigens. peroxidase conjugates on the basis of mcab eff ... | 1991 | 1724861 |
| pneumonia. patient profiles, choice of empiric therapy, and the place of third-generation cephalosporins. | choosing appropriate antimicrobial therapy for patients with pneumonia requires knowledge of the etiologic agents seen in specific kinds of patients at specific times and places. for community-acquired pneumonia, there is an important difference in the agents seen in the normal and the compromised host. the normal host most often presents with viral, mycoplasmal, or pneumococcal pneumonia. the exact place of chlamydia pneumoniae is still under study. a normal host who aspirates is at risk of ana ... | 1992 | 1730186 |
| spontaneous pasteurellosis in captive rocky mountain bighorn sheep (ovis canadensis canadensis): clinical, laboratory, and epizootiological observations. | we observed clinical signs, compared adrenal responses, and performed diagnostic tests on 12 captive rocky mountain bighorn sheep (ovis canadensis canadensis) during a spontaneous outbreak of pasteurellosis. cortisol in urine and feces was measured for bighorns sampled three times between 20 october and 1 november 1986. by 6 november, four of these had developed pneumonia, four showed only mild rhinitis, and four remained clinically normal. bighorns that ultimately developed pneumonia showed ele ... | 1991 | 1758018 |
| pcr test for chlamydia psittaci. | | 1991 | 1760023 |
| [detection of chlamydia trachomatis by polymerase chain reaction]. | a polymerase chain reaction (pcr) procedure was developed for detection of chlamydia trachomatis. two oligonucleotide primers based on sequences within the major outer membrane protein gene from c. trachomatis serovar l2 were used. a single dna fragment was amplified, when c. trachomatis dna was template for the pcr. no amplified product was detected in chlamydia psittaci dna, chlamydia pneumoniae dna or other bacterial dnas. the amplified dna fragment was detected, when dna of greater than or e ... | 1991 | 1761897 |
| abortion in japanese cows caused by chlamydia psittaci. | an outbreak of abortion in cows occurring in niigata prefecture was shown to be caused by chlamydia psittaci. elementary bodies characteristic of chlamydia were found in the liver of aborted fetuses and c. psittaci antigen was demonstrated by indirect immunofluorescence. chlamydia was isolated from the liver of aborted fetuses by the yolk sac inoculation of developing chick embryos and by the intraperitoneal inoculation of guinea pigs. abortion occurred mostly in middle or late pregnancy. aborte ... | 1991 | 1771749 |
| detection and strain differentiation of chlamydia psittaci mediated by a two-step polymerase chain reaction. | specific and sensitive amplification of major outer membrane protein (momp) gene dna sequences of chlamydia psittaci was achieved in a two-step polymerase chain reaction. first, oligonucleotide primers specific for 5' and 3' nontranslated regulatory regions of the momp gene were used in a polymerase chain reaction to amplify a dna fragment of approximately 1,400 bp. a portion of this dna fragment was amplified in a second reaction using a degenerate oligonucleotide primer specific for a dna sequ ... | 1991 | 1774323 |
| immune responses of the ovine lymph node to chlamydia psittaci. a cellular study of popliteal efferent lymph. | the popliteal efferent lymphatics were cannulated in sheep of two categories, seronegative or immune to chlamydia psittaci. following subcutaneous injection of live c. psittaci or control material into the draining area of the popliteal node, sequential samples of efferent lymph were collected and analysed. both categories of sheep responded to c. psittaci with increased outputs of lymphocytes and blast cells. numbers of blast cells rose both absolutely and as a proportion of the total. plasmabl ... | 1991 | 1779041 |
| [chlamydia infections in sheep and goats. with a reference to its significance as a zoonosis]. | infections by chlamydia psittaci are found worldwide in many species of mammal, including man, and birds. small ruminants are affected mainly with abortion in the later phase of gestation. additionally pneumonia and lesions in other organs can be observed. pathological and clinico-epidemiological aspects of the infection with c. psittaci in small ruminants are described and discussed. the description of a case of conjunctivitis acquired by one of the authors through contact with infectious mater ... | 1991 | 1796463 |
| [enzootic abortion in a goat herd, caused by mixed infection with coxiella burnetii and chlamydia psittaci. case report]. | we report on an outbreak of enzootic abortion in a herd of goats with high losses caused by mixed infection with coxiella burnetii and chlamydia psittaci. both agents could be detected by microscopic investigation of smears from afterbirths by giménez staining and by a capture enzyme-linked immunofluorescence assay (capture elifa). additionally, coxiella burnetii was isolated from the same tissues in bgm cell cultures. serological investigations with cf, indirect elifa, and if tests revealed hig ... | 1991 | 1796465 |
| serological assessment of chlamydial infection in the koala by a slide eia technique. | a rapid and simplified slide enzyme immunosorbent assay (eia) was developed for the diagnosis of chlamydial infection in the koala. hela 229 cells infected with koala strain chlamydia psittaci were fixed on the surface of multiwell slides and used as the antigen. the assay consisted of first reacting koala antiserum with the fixed c psittaci antigen, followed by reaction with biotinylated rabbit anti-koala igg, abc reagent and substrate. the chlamydial eia antibody titres obtained were compared ... | 1991 | 1807246 |
| [materno-fetal infection by chlamydia psittaci transmitted by the goat: a new zoonosis?]. | a case report is given of spontaneous abortion at 32 weeks brought about by chlamydia psittaci following contact with a herd of goats. severe symptoms were observed post-natally in this woman. nine cases of materno-foetal infection with this pathogen are known, however, the source of infection is usually ewes suffering abortive chlamydiosis. goats may also be infected, but caprine origin for the disease in pregnant women has not been reported previously. | 1991 | 1819408 |
| nucleotide sequence of a gene encoding a new genus specific protein of chlamydia psittaci. | dna fragment no. 13 from the c. psittaci pigeon strain, has been cloned in the plasmid puc19. hybridization analysis revealed that the fragment maintained a chlamydial common sequence. furthermore, nucleotide sequencing identified two partial open reading frames (orf), 675b. p. and 530b. p. expression of orfs revealed that the second orf encoded 25kd polypeptide, whereas the first orf did not produce any antigenic product. the 25kd beta-galactosidase fusion protein reacted strongly with chlamydi ... | 1991 | 1821438 |
| characterization of dna fragment from chlamydia psittaci avian strain which shows high homology with hypb gene of chlamydia. | a study was performed to characterize dna fragment no. 17 of c. psittaci strain p-1041 which encoded 42 kd beta-galactosidase fusion protein with type-specific antigenicity. sequence determination identified a partial open reading frame that spanned about 1,200b. p. nucleotides. screening the literatures for the nucleotide and deduced amino acid sequences revealed extensive similarity between the dna fragment of p-1041 and two chlamydial hypb genes. this dna showed 91.5% homology with c. psittac ... | 1991 | 1821439 |
| antigenic analysis of feline and bovine chlamydia psittaci with monoclonal antibodies. | monoclonal antibodies were established for antigenic analysis of feline and bovine chlamydia psittaci. the monoclonal antibodies recognized lipopolysaccharide (lps), 56-64, 84 or 86 kda antigens. at least 5 antibody-binding sites were detected on lps with the monoclonal antibodies. the 56-64 kda antigen was suggested to have both polypeptide and carbohydrate antibody binding sites. immunoblotting analysis of cat and cattle sera indicated that the 56-64 kda antigen is an important antigen in host ... | 1991 | 1830787 |
| antigenic analysis of avian chlamydia psittaci using monoclonal antibodies to the major outer membrane protein. | monoclonal antibodies to the major outer membrane protein (momp) of chlamydia psittaci derived from a parrot were established for antigenic analysis of avian c. psittaci. with 17 monoclonal antibodies to momp, 17 reactivity patterns were identified on 112 strains of c. psittaci, c. pneumoniae and c. trachomatis, which were isolated from birds, mammals and humans in japan, u.s.a., canada and taiwan, from 1938 to 1987. immunological reactivity of budgerigar-derived strains to the monoclonal antibo ... | 1991 | 1832026 |
| kinetic studies on the appearance of antigens of chlamydia psittaci during its developmental cycle. | the kinetics of the antigen production of chlamydia psittaci strains izawa-1 and pigeon-1041 (p-1041) was examined every 6 hr after infection up to 48 hr, by the indirect immunofluorescent antibody technique using monoclonal antibodies (mabs). all three genus-specific antigenic determinants on lipopolysaccharide (lps) appeared during the whole growth cycle. antigenic determinants on proteins were, on the other hand, detected at various time periods from the early to the late stages of infection. ... | 1991 | 1834211 |
| sequence analysis of the major outer membrane protein gene of chlamydia pneumoniae. | compared with the major outer membrane proteins (momps) of the other chlamydial species, the chlamydia pneumoniae momp appears to be less antigenically complex, and as determined by immunoblot analysis, it does not appear to be the immunodominant antigen recognized during infection. nucleotide sequence analysis of the c. pneumoniae momp gene (ompa) revealed that it consisted of a 1,167-base open reading frame with an inferred 39,344-dalton mature protein of 366 amino acids plus a 23-amino-acid l ... | 1991 | 1840574 |
| comparison of avian chlamydia psittaci isolates by restriction endonuclease analysis and serovar-specific monoclonal antibodies. | avian chlamydia psittaci isolates were examined by restriction endonuclease analysis and serovar-specific monoclonal antibodies and compared with ovine abortion and polyarthritis isolates. the avian isolates were divided into four serovars (turkey, psittacine, pigeon, and duck) based on their reactivity to the monoclonal antibodies. the dna digest patterns were similar across the four avian serovars; most bands were identical when the isolates were tested with psti, bamhi, and ecori restriction ... | 1991 | 1848867 |
| cloning and sequence analysis of the major outer membrane protein gene of chlamydia psittaci 6bc. | the gene encoding the major outer membrane protein (momp) of the psittacine chlamydia psittaci strain 6bc was cloned and sequenced. n-terminal protein sequencing of the mature momp indicated that it is posttranslationally processed at a site identical to the site previously identified in the momp of chlamydia trachomatis l2. the nucleotide sequence of the c. psittaci 6bc momp gene was found to be 67 to 68% identical to those of human c. trachomatis strains, 73% identical to that of chlamydia pne ... | 1991 | 1856001 |
| genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene. | the 60-kda cysteine-rich outer membrane protein genes of chlamydia psittaci, chlamydia pneumoniae, and chlamydia trachomatis have very different 5' ends, but two areas flanking this variable region show absolute sequence conservation. this observation permitted differentiation of the three species of chlamydia by the polymerase chain reaction (pcr), forming the basis of a diagnostic test for chlamydial infections. the pcr product containing the variable region of the respective 60-kda crp genes ... | 1991 | 1864938 |
| [anti-chlamydia psittaci antibodies in a healthy pediatric population]. | psittacosis or ornithosis is a bird disease caused by chlamydia psittaci which can be transmitted to man. little is known of the epidemiology of the disease in this region, but occasional cases which have been brought to the authors' attention have prompted an epidemiological study on the subject. the preliminary results indicate a surprising percentage, over 8%, of antibodies in infants and children. this percentage varies little in relation to place of residence, rural or urban, or the presenc ... | 1991 | 1870537 |
| evaluation of an immunofluorescence test on direct smears of conjunctival and urogenital swabs taken from koalas for the detection of chlamydia psittaci. | the chlamydia-cel vet if test (ccvit), a commercially available immunofluorescence test for use on direct smears of clinical specimens, was evaluated in a colony of 43 captive koalas. the test is based on a monoclonal antibody directed against the chlamydial common group specific lipopolysaccharide antigen. swabs were taken from conjuncitva and penis or urogenital sinus and used for direct smear evaluation and cell culture isolation. compared with isolation of the organism in cell culture, the c ... | 1991 | 1883293 |
| chlamydial infection in a colony of captive koalas. | forty three koalas in a captive colony were investigated for the presence of chlamydia psittaci infection and associated disease. swabs were taken from conjunctivae and urogenital sites for cell culture isolation of c psittaci and for cytological examination (direct smears) for chlamydial inclusions and evidence of inflammation. on the basis of cell culture isolation, 28 samples from 25 koalas were positive for c psittaci (that is, infected). three koalas were positive from both sites, 5 from co ... | 1991 | 1883294 |
| chlamydia pneumoniae elementary body antigenic reactivity with fluorescent antibody is destroyed by methanol. | methanol, used for fixing chlamydia organisms to microscopic slides, was shown to eliminate the antigenic reactivity of chlamydia pneumoniae elementary bodies with fluorescent antibodies. acetone fixation had no adverse effect. chlamydia trachomatis and chlamydia psittaci were not affected by methanol fixation. the effect of methanol on c. pneumoniae is another characteristic that differentiates this species from the other two chlamydia species. | 1991 | 1885752 |
| serotyping of chlamydia psittaci isolates using serovar-specific monoclonal antibodies with the microimmunofluorescence test. | a panel of 10 serovar-specific monoclonal antibodies that could distinguish 10 distinct serovars of chlamydia psittaci was prepared. the panel included one monoclonal antibody to each of the 10 serovars. monoclonal antibodies were selected for their specificity in the indirect microimmunofluorescence test. each of the monoclonal antibodies had a titer of 1:1,280 or higher to the homologous strain, with only two showing any cross-reactivity at a dilution of 1:10. chlamydial antigen derived from o ... | 1991 | 1890172 |
| [simultaneous infection by coxiella burnetii and chlamydia psittaci in 2 patients: the problem of psittacosis of non-avian origin]. | | 1991 | 1895793 |
| pcr test for chlamydia psittaci. | | 1991 | 1897094 |
| the use of fluorescein-conjugated monoclonal antibodies, cell culture and transmission electron microscopy to detect chlamydia psittaci and associated lesions in experimentally infected mice. | an immunofluorescence test based on a monoclonal antibody (mab) was used to demonstrate chlamydiae in formalin-fixed and paraffin wax-embedded tissues from 10 adult mice experimentally infected by the oral route with chlamydia psittaci isolated from the fetal membranes of an aborted ovine fetus. samples of lung, jejunum and spleen were examined by bright-field microscopy, immunofluorescence and transmission electron microscopy, and were cultured for chlamydia in mccoy cells. these tissues were c ... | 1991 | 1918451 |
| ovine psittacosis and sarcoidosis in a pregnant woman. | a woman in the first trimester of pregnancy presented with pneumonia and hilar lymphadenopathy after exposure to lambing ewes. she subsequently aborted. infection with chlamydia psittaci of ovine origin was confirmed. pregnant women are susceptible to this infection, which may cause life threatening disease. the patient also had features of sarcoidosis, and the two conditions ran a similar time course. there is a possibility that ovine psittacosis caused an illness indistinguishable from sarcoid ... | 1991 | 1926035 |
| gamma interferon-activated human macrophages and toxoplasma gondii, chlamydia psittaci, and leishmania donovani: antimicrobial role of limiting intracellular iron. | iron-saturated transferrin did not reverse the intracellular killing or inhibition of toxoplasma gondii, chlamydia psittaci, or leishmania donovani by gamma interferon-activated human macrophages. deferoxamine, an iron chelator, also did not impair replication within unstimulated macrophages. limiting the availability of intracellular iron is an unlikely mechanism in human macrophage activity against these three diverse pathogens. | 1991 | 1937829 |
| [meningism as the main symptom of chlamydia psittaci infection]. | | 1991 | 1944716 |
| [bacteriologic studies of the occurrence of chlamydia psittaci in organs of swine and in aborted swine fetuses]. | by inoculation of embryonating chicken eggs via the yolk sac route chlamydia psittaci was grown from 11 lungs of 45 pigs with pneumonia (24.4%). from the lungs of 55 pigs with other diseases the organism was isolated in five cases (9.1%). chlamydiae were not detectable by cultural methods in the uterine mucosa of 87 sows, arthritic joints of 30 store pigs and in aborted fetuses. a commercial available enzyme amplified immunoassay indicated the presence of chlamydial antigen in mucosal scrapings ... | 1991 | 1950250 |
| the antigenic specificity of the humoral immune response to primary and repeated ocular infections of the guinea pig with the gpic agent (chlamydia psittaci). | the antigenic specificity of the humoral immune response in guinea pigs to primary and repeated ocular infections with the guinea pig inclusion conjunctivitis (gpic) chlamydial agent was analysed using microbiological, serological and western blotting techniques. the results indicate that although there was a response to many minor polypeptide antigens, there was a marked lack of reactivity to the major outer membrane protein (momp), particularly following reinfection of guinea pigs. it is sugge ... | 1991 | 1955051 |
| atypical pneumonia. | the atypical pneumonia syndrome usually implies a benign illness where systemic complaints predominate over respiratory symptoms. cough is prominent; chest radiographic findings are varied. many organisms are associated with this syndrome. mycoplasma pneumoniae, chlamydia psittaci, chlamydia pneumoniae, coxiella burnetii, and francisella tularensis are reviewed in this article. | 1991 | 1955701 |
| ocular chlamydial zoonosis: an experimental study in monkeys. | two locally isolated strains of chlamydia psittaci from faeces of apparently healthy sheep and conjunctiva of holstein heifers suffering from conjunctivitis and pneumonia were used for ocular experimental studies in 7 rhesus monkeys. titrated (eld50 10(3.6)/0.2 ml and 10(4.5)/0.2 ml) partially purified yolk sac suspension of each strain was instilled onto conjunctiva of each animal. the clinical and laboratory studies were followed till 8 weeks post-infection. both the strains produced trachoma ... | 1991 | 1959319 |
| diagnosis of pneumonia by cultures, bacterial and viral antigen detection tests, and serology with special reference to antibodies against pneumococcal antigens. | in a prospective study of the etiology of pneumonia 196 adult patients were included. one of the following criteria was required for diagnosis of pneumococcal pneumonia: isolation of pneumococci from blood; isolation from transtracheal aspirate; isolation from sputum or nasopharynx or detection of capsular antigen in sputum in combination with a significant increase in antibodies against at least one pneumococcal antigen (type-specific capsular polysaccharide, c-polysaccharide, pneumolysin); or ... | 1991 | 2019757 |
| comparison of isolates of chlamydia psittaci of ovine, avian and feline origin by analysis of polypeptide profiles from purified elementary bodies. | the single species chlamydia psittaci is a diverse grouping which contains several different types of chlamydial strain for which there is no generally accepted typing method. the results obtained when profiles of polypeptides from purified elementary bodies are compared are consistent with type designations obtained using other criteria. however, the method still requires large scale culture and extensive purification of the chlamydial cells. | 1991 | 2024446 |
| detection of chlamydia psittaci from avian field samples using the pcr. | | 1991 | 2028566 |
| psittacosis and arthritis. | recent findings justify the opinion that chlamydia psittaci is the reappearance of a forgotten pathogen. the clinical manifestation and the course of psittacosis are extremely variable, whereas the clinical spectrum of the infection with the different strains of c. psittaci is not known. reactive arthritis during the course of psittacosis has been rarely described in humans. however, it has been stated that c. psittaci could be added to the list of infectious agents able to induce reactive arthr ... | 1991 | 2032470 |
| detection of chlamydia psittaci using dna probes and the polymerase chain reaction. | fewer than 10(5) elementary bodies of chlamydia psittaci could be detected by using dna hybridisation with a plasmid probe specific for avian chlamydial strains. pcr amplification of chlamydial dna using primers specific for conserved regions of the major outer membrane protein gene enabled the detection of fewer than 10 elementary bodies. dna could be amplified from 22 of the 24 chlamydial strains tested including avian, feline, ovine, caprine, koala and lymphogranuloma venereum strains. | 1991 | 2037227 |
| sequence analysis and lipid modification of the cysteine-rich envelope proteins of chlamydia psittaci 6bc. | the envelopes of elementary bodies of chlamydia spp. consist largely of disulfide-cross-linked major outer membrane protein (momp) and two cysteine-rich proteins (crps). the momp gene of chlamydia psittaci 6bc has been sequenced previously, and the genes encoding the small and large crps from this strain were cloned and sequenced in this study. the crp genes were found to be tandemly arranged on the chlamydial chromosome but could be independently expressed in escherichia coli. the deduced 87-am ... | 1991 | 2050637 |
| towards a differential polymerase chain reaction test for chlamydia psittaci. | | 1991 | 2053263 |
| hepatitis in a family infected by chlamydia psittaci. | hepatic involvement is considered a rare complication of psittacosis. occurrence of icteric hepatitis as the cardinal manifestation of the disease has been rarely reported. we describe two members of a family infected by psittacosis, in whom icteric hepatitis was the prominent expression of the disease. the diagnosis of psittacosis was confirmed by isolation of the pathogen and by detection of serum antibodies to chlamydia species. no serological evidence for acute twar infection was found. chla ... | 1991 | 2061902 |
| detection of chlamydia trachomatis in clinical specimens by the polymerase chain reaction. | sequences derived from the endogenous plasmid of chlamydia trachomatis and from the genes coding for ribosomal 16s rna of chlamydia psittaci were used as primers and oligonucleotide probes for detection of chlamydiae by the polymerase chain reaction. the endogenous plasmid primers generated specific amplified products of 517 bp with all known chlamydia trachomatis serovars. no specific products of chlamydia psittaci and chlamydia pneumoniae could be detected using these primers. with the rrna pr ... | 1990 | 2073896 |
| [chlamydia isolation from a large aviary for birds of prey]. | antigen against chlamydia psittaci was detected in 31% to 41% of healthy birds of prey (accipitriformes, falconiformes, and strigiformes) by examination of cloacal swabs and faeces out of 119 birds with antigen-elisa and cell culture. chlamydia psittaci-antibodies could be demonstrated in 34% of birds by cf, in 74% by belisa, and in 71% by chlamydia-antibody-elisa (chlamydia-ak-eia). changes of governmental regulations are discussed. | 1990 | 2082626 |
| [seroprevalence of chlamydia psittaci in rural and urban populations of the province of madrid]. | | 1990 | 2089217 |
| chlamydial infection in aborted and stillborn lambs. | chlamydia psittaci is a major cause of ovine abortion in the fourth to fifth months of gestation. during the lambing seasons of 1986, 1987, and 1988, fetuses from 52 cases of ovine abortion, stillbirth, or perinatal death were submitted to the laboratory for necropsy examination. placenta or fetal tissues from 34 cases were cultured on mouse l cells for c. psittaci. chlamydia psittaci was identified by immunofluorescence on cultures in 20 of these cases. the major gross lesion consistently assoc ... | 1990 | 2090268 |
| case for diagnosis. necrotizing granulomatous cholangiohepatitis with multifocal caseonecrotic granulomas caused by chlamydia psittaci organisms and a concomitant enteric infection. | | 1990 | 2107470 |