| phagocytic and chlamydiae-inhibiting activities of stimulated and nonstimulated periotneal mouse macrophages. | phagocytic and chamydiacidal properties of nonstimulated and stimulated mouse mononuclear cells for two chlamydia psittaci 6bc strains were investigated. it was determined that macrophages kept in monolayer culture (i.e. stimulated phagocytes) developed much more efficient chlamydiacidal ability than did cells kept in suspension directly after harvest (i.e. nonstimulated phagocytes). a thousandfold decrease of chlamydial infectivity was observed 60 min after induction of phagocytosis in stimulat ... | 1976 | 986871 |
| ornithosis in domestic pigeons gone wild in bratislava. | | 1976 | 987103 |
| ornithosis in domestic pigeons gone wild in bratislava. | | 1976 | 987114 |
| some physical properties of a sheep pneumonia strain of chlamydia psittaci. | | 1976 | 997192 |
| ultrastructural changes in host cellular organelles in the course of the chlamydial developmental cycle. | different stages in the chlamydial developmental cycle were correlated with cytopathic changes in host cellular organelles by ultrastructural analysis of infected cultured cells and cells of the intestinal mucosa of newborn calves. four distinct morphologic forms of chlamydial development can be distinguished: elementary bodies, dispersing forms, reticulate bodies and condensing forms which proceed to form elementary bodies. from the stage of elementary body uptake through formation and multipli ... | 1976 | 1015025 |
| antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. i. antigenic heterogeneity between c. trachomatis and c. psittaci. | tw0-dimensional immunoelectrophoresis was used for the antigenic analysis of chlamydia. antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent triton x-100. nineteen and 16 distinct components were observed for chlamydia trachomatis strain l2/434/bu and c. psittaci strain meningopneumonitis/cal-10, respectively. the triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed ... | 1975 | 1080772 |
| chlamydiae as agents of sexually transmitted diseases. | chlamydiae are being increasingly recognized as an important cause of human disease. the known geographical distribution of lymphogranuloma venereum and the role of chlamydiae as agents of sexually transmitted diseases are reviewed. the presence of chlamydiae in the urethra and the cervix, and their etiological relationship to genital infections, first recognized in connexion with ocular infections, have been proved in a number of studies in selected populations in a few countries. chlamydiae ap ... | 1976 | 1088107 |
| [detection of the antigens of the causative agent of enzootic ovine abortion by an immunoperoxidase method]. | | 1975 | 1108388 |
| [study of the specificity of various classes of immunoglobulins (igm and igg) v techenie ornitoznoĭ infektsii]. | studies of the specificity of immunoglobulins of different classes in the course of both experimental and natural orinthosis infection showed igg antibody to possess a wide spectrum of activity: group-, species- and strain-specific, but specificity to group antigenic components was predominant. igm antibodies were species-specific, however, population of gammam-immunoglobulins of animal sera in approximately 7 and 14 days after infection antibody suggested a method for the study of the antigenic ... | 1975 | 1108451 |
| chlamydiosis in a beef herd. | chlamydial infection in a large beef herd is illustrated and discussed. the pre-natal and post-natal losses that occurred during 1972 are highlighted. total pre-natal losses for the nine calving herds comprising 2,915 animals varied between 3,7% and 12,4%. between 1,2% and 11,4% of the calves born alive in the nine herds died before weaning with 70% of the losses occurring within the first three weeks of life. chlamydial organisms were demonstrated in pre-natal and post-natal losses from all nin ... | 1975 | 1195268 |
| [obtaining hyperimmune serum for the diagnosis of enzootic ovine abortion]. | | 1975 | 1209918 |
| insidious endocarditis caused by chlamydia psittaci. | | 1975 | 1212580 |
| [micromethod for the ha and hi tests in ornithosis]. | the possibility of using the ha and hi tests in micromethod modifications in ornithosis was studied. the hemagglutinating activity of various antigens was tested. by the employment of the micromethod antihemagglutinins were found in sera from patients, immunized animals (donkey and guinea pigs) as well as from pigeons and ducks. no antibody was found in control sera and sera from tortoises. the results of the ha and hi tests performed by macro- and micromethod were similar. the micromethod is as ... | 1975 | 1216842 |
| [production of a noninfectious ornithosis hemagglutinin preparation]. | one of the main obstacles to introduction of the hi test in to extensive use is the high infectivity of the causative agent suspensions used as the source of hemagglutinin (ha). the sterilizing effect of uv-rays, high temperature (56 -60 degrees c) and beta-propiolactone was studied in attempts at preparation of noninfectious ornithosis ha. only bpl was found to be effective. by this method of treatment of the allantoic fluid a noninfectious preparation of ornithosis ha was obtained which was hi ... | 1975 | 1226714 |
| [experimental infection of guinea pigs with chlamydia. 1. pathomorphological studies]. | the changes that occurred following nasal instillation of a bovine strain of chlamydia psittaci were characterized by hypertrophy and proliferation of epithelial cells and exudates containing polymorphonuclear leukocytes, with acute bronchopneumonia and less intense inflammatory reactions in nasal and tracheal mucosa, spleen and pulmonary lymph nodes. the intestines, liver, kidney and brain were scarcely affected. there was little prospect of complete recovery from the clinically mild, or even s ... | 1975 | 1227428 |
| ultrastructural studies of chlamydia psittaci 6bc in situ in yolk sac explants and l cells: a comparison with gram-negative bacteria. | chlamydia psittaci (6bc) was grown in yolk sac explants and in l cells and fixed by perfusion in situ to provide undamaged material for comparison with gram-negative bacteria. reticulate, intermediate, and elementary bodies were all seen to lack a well-defined periplasmic space; intermediate and elementary bodies showed condensations of the nucleoid which differ from common bacterial configurations; and the cytoplasm of highly condensed elementary bodies was much more electron dense than that of ... | 1975 | 1238156 |
| the effect of purification on the ultrastructure and infectivity of egg-attenuated chlamydia psittaci (6bc). | a procedure is described for the purification of mixed populations of the three different morphological forms of chlamydia psittaci (6bc) from infected yolk sac membranes. elementary bodies and small intermediate bodies are not perceptibly damaged during purification which involves homogenization of the host cells, differential centrifugation, sedimentation through 20% sucrose, and treatment with trypsin. the observation that elementary bodies undergo plasmolysis in 20% sucrose is interpreted as ... | 1975 | 1238157 |
| detection of igm antibodies to chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci from japanese infants and children with pneumonia. | chlamydia trachomatis (c. trachomatis) is now well established as a pathogen of neonatal inclusion conjunctivitis and infantile pneumonia. c. pneumoniae (twar) and c. psittaci also cause pneumonia and other respiratory infections. serum samples from 223 japanese infants and children with pneumonia were tested for igm antibodies to c. trachomatis, c. pneumoniae and c. psittaci. igm antibodies to c. trachomatis were measured by an enzyme immunoassay (eia) and by a microimmunofluorescence (mif) tes ... | 1992 | 1296808 |
| chlamydia pneumoniae respiratory infection in a child--a case report. | a case of respiratory infection in a child due to chlamydia pneumoniae is reported. the diagnosis was made by the detection of chlamydial antigen in the tracheal secretion and a significant increase in c. pneumoniae antibody titre. the infection responded well to erythromycin therapy. | 1992 | 1303485 |
| purification of chlamydia psittaci antigen by affinity chromatography on polymyxin b agarose for use in the enzyme-linked immunosorbent assay (elisa). | the purification of cell wall antigens of chlamydia psittaci by affinity chromatography on polymyxin b agarose is described. chlamydial cell wall antigens were prepared using different methods: heat treatment, ultrasonication and sodium deoxycholate treatment. the antigens were subsequently purified by gel chromatography. the highest amount of cell wall antigens was obtained by heat treatment of the chlamydiae at 90 degrees c and ph 8.5. the purified antigens showed molecular weights of 450 kda ... | 1992 | 1303687 |
| evaluation of the polymerase chain reaction (pcr) for detection of chlamydia psittaci in abortion material from ewes. | the polymerase chain reaction (pcr) was evaluated as a diagnostic tool for detection of chlamydia (c.) psittaci in abortion material from 40 ewes. for this purpose, pcr results of 87 samples were compared with direct microscopic identification after chemical staining, cell culture isolation and a commercially available enzyme-linked immunosorbent assay (elisa). the value for sensitivity as compared to cell culture was 97.7% whereas the specificity-value was calculated to be 84.1%. | 1992 | 1303688 |
| in vitro activities of five quinolones against chlamydia pneumoniae. | the in vitro susceptibilities of 10 strains of chlamydia pneumoniae were determined for five quinolones, including ciprofloxacin, ofloxacin, fleroxacin, temafloxacin, and sparfloxacin. sparfloxacin was the most active compound tested, followed by ofloxacin and temafloxacin. ciprofloxacin and fleroxacin were the least active. the use of hep-2 cells for testing c. pneumoniae resulted in larger inclusions but essentially the same endpoints as were seen with use of hela 229 cells. | 1992 | 1320366 |
| in vitro activities of azithromycin, clarithromycin, l-ofloxacin, and other antibiotics against chlamydia pneumoniae. | the in vitro susceptibilities of 11 strains of chlamydia pneumoniae to azithromycin, clarithromycin, erythromycin, l-oflaxacin, and doxycycline were determined. clarithromycin was the most active agent tested, with an mic for 90% of strains and minimal chlamydiacidal concentration for 90% of strains of 0.03 microns/ml. the activity of azithromycin was similar to those of erythromycin and doxycycline, with mics for 90% of strains of 0.125 to 0.25 microns/ml. however, the prolonged half-life and e ... | 1992 | 1324650 |
| [pneumopathies caused by chlamydia pneumoniae]. | among the atypical pneumonias observed between march 1990 and march 1991, 6 were diagnosed as being caused by chlamydia pneumoniae of the twar strain. the serological diagnosis was obtained by a microimmunofluorescence test. all 6 patients had anti-twar antibody levels higher than 512; they were treated with a macrolide administered by the oral route and were cured without sequelae or recurrences. four cases received a ten day course of roxithromycin 300 mg/day and one case received erythromycin ... | 1992 | 1326110 |
| similarity of chlamydia pneumoniae strains in the variable domain iv region of the major outer membrane protein gene. | dna was amplified by polymerase chain reaction from the gene encoding the major outer membrane protein (momp) of chlamydia pneumoniae in order to examine the relatedness of strains isolated from diverse geographical regions. primers for this reaction were chosen to span a 207-bp region comparable to that of the fourth variable segment of the momp gene of chlamydia trachomatis. among c. trachomatis, sequence heterogeneity is characteristic within variable sequence domain iv (vdiv) and correlates ... | 1992 | 1339411 |
| [biological products and laboratory reagents dehydrated by a rapid direct vacuum desiccation method. vi. studies of the preservation of microbial strains (c. burnetii, chlamydia psittaci, mycoplasma pneumoniae) and a laboratory reagent (para-nitrophenyl phosphate) by vacuum desiccation]. | | 1992 | 1340260 |
| chlamydia pneumoniae infection and asthma. | | 1992 | 1349397 |
| chlamydia and complexes. | | 1992 | 1349943 |
| myocarditis caused by chlamydia pneumoniae (twar) and sudden unexpected death in a swedish elite orienteer. | | 1992 | 1353578 |
| prevalence of specific antibodies to chlamydia pneumoniae (twar) in swedish orienteers. | | 1992 | 1357445 |
| biological properties and genetic analysis of the ompa locus in chlamydiae isolated from swine. | eight strains of chlamydia psittaci isolated from swine with pneumonia, pleuritis, pericarditis, and enteritis were characterized through analysis of the major outer membrane protein gene ompa by a two-step polymerase chain reaction, by their interactions with cells in culture, and by the morphologic features and ultrastructure of intracellular inclusions. amplified chlamydial ompa dna fragments were differentiated by restriction endonuclease digestion. chlamydial isolates were separated into 2 ... | 1992 | 1358014 |
| pcr detection and differentiation of chlamydia pneumoniae, chlamydia psittaci and chlamydia trachomatis. | a pcr-based system was developed for the detection and differentiation of chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae. a conserved 145 bp fragment of the chlamydial omp1 gene was amplified from all three species. the three species were then differentiated from each other by digestion of this pcr product with restriction enzymes eco ri and either hind iii or pst i. the system was shown to work for two strains of c. pneumoniae, 11 strains of c. psittaci and 10 serovars of c. ... | 1992 | 1361961 |
| distinguishing chlamydia species by restriction analysis of the major outer membrane protein gene. | clinical isolates of chlamydia pneumoniae from diverse geographic locations and strains of other chlamydia species were typed by polymerase chain reaction (pcr) amplification of the major outer membrane protein (momp) gene followed by restriction fragment length polymorphism analysis of the product. use of synthetic primers corresponding to highly conserved regions of the momp gene resulted in amplification of a 1070 bp product in laboratory strains and clinical isolates of c. pneumoniae, c. tra ... | 1992 | 1361962 |
| identification of chlamydia pneumoniae by dna amplification of the 16s rrna gene. | chlamydia pneumoniae is an important cause of respiratory disease in humans, but diagnosis of c. pneumoniae is hindered by difficulties in the in vitro growth of the organism. in order to improve detection and identification, we recently developed a polymerase chain reaction (pcr) assay which uses oligonucleotide primers specific for c. pneumoniae. the nucleic acid sequence was determined for the 16s rrna of c. pneumoniae, and regions in which c. pneumoniae differed from both chlamydia psittaci ... | 1992 | 1374077 |
| immunoelectron microscopy of chlamydia psittaci with monoclonal antibodies. | an immunoelectron microscopic study was performed to determine the distribution of antigenic components on particles of chlamydia psittaci and infected cells using a number of monoclonal antibodies (mabs). of three anti-lipopolysaccharide (lps) antibodies (4d5, a2 and 4g5), two antibodies (4d5 and a2) reacted with the surface of reticulate bodies (rbs) but not with that of elementary bodies (ebs). the other antibody (4g5) reacted with both ebs and rbs. examination of infected cells in thin secti ... | 1992 | 1374830 |
| detection and antigenicity of chlamydial proteins that bind eukaryotic cell membrane proteins. | chlamydia psittaci proteins capable of binding eukaryotic cell membranes were identified and antigenically characterized. cell membrane proteins (cmp) of noninfected cells were labeled with biotin (b-cmp), then were extracted with 1% triton x-100. nitrocellulose membrane strips containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis-resolved proteins of chlamydial elementary bodies (eb) were reacted with the b-cmp extract, followed by addition of streptavidin-conjugated horse radish ... | 1992 | 1378251 |
| keratitis in free-ranging koalas (phascolarctos cinereus) on magnetic island, townsville. | seventy free-ranging koalas (phascolarctos cinereus) from magnetic island (queensland, australia) underwent an ocular examination, blood collection and serological examination for chlamydia psittaci antibodies, and an examination of their teeth and genitalia. in 12 koalas long-standing unilateral keratitis was noted and in another 10 animals long-standing bilateral keratitis was observed. all animals were seronegative for chlamydia psittaci and apart from some nasal discharge and a variety of as ... | 1992 | 1380994 |
| prevalence of chlamydia pneumoniae antibodies in hungary. | investigation of chlamydia pneumoniae (twar) antibodies in paired sera of 120 patients with various respiratory diseases revealed a prevalence of 4.2% of igg seroconversion. igg antibody without seroconversion was found in 83.3%. sera of ten patients showed titers as high as 512-1024 or above. children with no respiratory disease and blood donors in budapest had specific igg in 46.5% and 75.2% respectively. prevalence of igg antibody in children from the rural areas of hungary was about 50% lowe ... | 1992 | 1396727 |
| outbreak of chlamydia pneumoniae infection in four farm families. | the epidemiology of chlamydia pneumoniae infection was studied in an outbreak in four farm families living close together in denmark. eleven of 20 members of the families studied had bronchitis or pneumonia characteristic of chlamydia pneumoniae infection. serologic evidence of chlamydia pneumoniae as causative agent was strengthened by a high incidence of epidemic infection. transmission within families and a high frequency of disease versus asymptomatic infection are two findings which deviate ... | 1992 | 1396769 |
| [an isolation procedure of chlamydia pneumoniae and chlamydia trachomatis]. | the author devised a method which permits simultaneous isolation and identification of chlamydia using a slide chamber with 8 wells. contaminating bacteria were eliminated by filtration with the membrane filter. the procedure allowed isolation of chlamydia pneumoniae and chlamydia trachomatis from 11 (10.1%) out of 109 and 10 (9.2%) out of 109 otolaryngologic clinical specimens, respectively. the use of hl cells together with hela-229 cells had been considered essential to isolate chlamydia from ... | 1992 | 1402079 |
| [morphology and immunotyping of ac-43 strain of chlamydia pneumoniae isolated from a japanese child]. | a strain of chlamydia pneumoniae (c. pneumoniae) which had been isolated from a japanese child (ac-43) was examined morphologically and serologically using micro-immunofluorescent (micro-if) test. inclusions of ac-43 were stained by an indirect immunofluorescent method using c. pneumoniae specific monoclonal antibody. they were dense round inclusions which had been reported as a characteristic figure for c. pneumoniae. an elementary body (eb) of ac-43 was pear-shaped by electron micrograph, whic ... | 1992 | 1402097 |
| [isolation of chlamydia pneumoniae from a patient with acute bronchitis]. | chlamydia was isolated from the throat of a 15-year-old male patient with acute bronchitis. the chlamydia isolate, yk-41, was stained with fitc-conjugated monoclonal antibodies specific to c. pneumoniae and the genus chlamydia, whereas staining of monoclonal antibody specific to c. trachomatis was negative. these results indicated that the strain yk-41 could be identified as c. pneumoniae. serum igm antibody against c. pneumoniae was detected in high titer in the patient in the acute phase using ... | 1992 | 1402098 |
| [comparison of sensitivity of hep-2 cells with that of hl cells against chlamydia pneumoniae]. | we compared the growth of chlamydia pneumoniae, reference strain tw183 and an isolate from a japanese infant, ac43 in hela229, hl and hep-2 cells. the mean number of inclusion-forming units was significantly higher on hl cells than hela229 cells, when the cells were not pretreated with deae-dextran. when the cells were not pretreated with deae-dextran, hep-2 cells had a higher mean number of inclusion-forming units and a higher yield than other cell lines. iscove's modified dulbecco medium enhan ... | 1992 | 1402106 |
| [psittacosis, only in birds?]. | | 1992 | 1405806 |
| human psittacosis linked to a bird distributor in mississippi--massachusetts and tennessee, 1992. | during april-may 1992, cdc was notified of a possible outbreak of psittacosis involving members of two families in massachusetts and tennessee who had recently purchased birds as pets. in the subsequent investigation of this problem, human psittacosis was defined as a fourfold rise in complement-fixing antibody titer to > or = 32 or a single titer of > or = 32 in a patient with fever and/or respiratory symptoms. this report summarizes the investigation of this problem. | 1992 | 1406575 |
| spread of subclinical chlamydia pneumoniae infection in a closed community. | chlamydia pneumoniae infections may spread subclinically. the present investigation took place in a military setting. sera drawn when the conscripts had entered their military service 2 months previously had been kept frozen and were available. in a camp with 500 people, 35 (7%) developed clinical symptoms of pneumonia. the infection was serologically verified with c. pneumoniae-specific micro-immunofluorescence technique. of 40 healthy controls, 21 turned out to fulfil the serological criteria ... | 1992 | 1411308 |
| seroprevalence of chlamydia pneumoniae in the normal population. | | 1992 | 1425723 |
| the prevalence and regional distribution of antibodies against chlamydia pneumoniae (strain twar) in finland in 1958. | the occurrence of nonavian 'ornithosis' was reported in scandinavia at the end of the 1950s. in order to find out whether chlamydia pneumoniae had been present in finland, we examined the igg antibody prevalence to c pneumoniae in samples representing the whole rural population of finland in 1958. the total number of sera studied using micro-immunofluorescence was 2000. trend-surface analysis was used to examine the regional patterns of antibody prevalence. c pneumoniae antibodies were present t ... | 1992 | 1428498 |
| [study on the bacteriological examination of sputum and bronchoscopy specimens from 31 cases with pneumonia due to chlamydia psittaci]. | we carried out the bacteriological examination of sputum and bronchoscopy specimens from 31 cases with pneumonia due to c. psittaci. the results obtained were as follows: 1. the positive culture of sputum and bronchoscopy specimens were 38.7% (12/31). 2. the organisms detected from them were 13 strains of gram-negative bacilli, 2 of gram-positive cocci and one gram-positive bacillus. 3. significant differences were observed in the white blood cell count between the cases of positive culture and ... | 1992 | 1431354 |
| culture of sheep chlamydia in a sheep fibroblast cell culture. | abortion and enteric isolates of chlamydia psittaci from sheep differed in their growth in a fibroblastic cell culture derived from the small intestine of a lamb. twenty abortion isolates, each from a different farm, produced large inclusions which could be passaged several times whereas 10 enteric isolates each from different farms (but from some of the farms of origin of the abortion isolates) produced sparse inclusions which could not be passaged. this appears to be a rapid method of distingu ... | 1992 | 1439220 |
| infections caused by chlamydia pneumoniae strain twar. | | 1992 | 1445972 |
| enzyme electrophoretic polymorphism differentiates invasive from non-invasive chlamydia psittaci ruminant isolates. | a group of 24 chlamydia psittaci strains isolated from ruminants, belonging to serotype 1 and previously classified as invasive in a mouse model of virulence, was compared to a group of 10 non-invasive strains belonging to serotype 2 by using determination of glucose-6-phosphate and l-malate dehydrogenase zymotypes resulting of the infection of cells by these strains. the serotype 1 or invasive isolates represent a homogeneous group by sharing a unique zymotype which was not observed in the non- ... | 1992 | 1448629 |
| establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the ideia chlamydia kit and dna probe by using the purified elementary bodies. | to evaluate the sensitivity of commercially available test kits for detection of chlamydiae, we established a method of purifying chlamydia trachomatis and chlamydia pneumoniae elementary bodies (ebs). we then subjected the purified ebs, together with the purified ebs of chlamydia psittaci, to the ideia chlamydia (ideia) and dna probe test kits to determine the eb numbers at the detection limits. the sensitivities of the test kits were thus compared. the results can be summarized as follows. (i) ... | 1992 | 1452662 |
| comparison of nine antigen detection kits for diagnosis of urogenital infections due to chlamydia psittaci in koalas. | chlamydia psittaci is the major cause of infectious disease in the koala (phascolarctos cinereus). it causes four disease syndromes in the koala, namely, conjunctivitis, rhinitis, cystitis, and infertility (females only). diagnosis of chlamydial infections in koalas relies primarily on isolation of the organism in cell culture. serology has generally not been useful, and little use has previously been made of the commercially available antigen detection kits. we examined the sensitivity, specifi ... | 1992 | 1452703 |
| high prevalence of chlamydia pneumoniae infection in children and young adults in spain. | | 1992 | 1454443 |
| isolation of chlamydia psittaci from pleural effusion in a dog. | | 1992 | 1457552 |
| preparation and use of a monoclonal antibody to detect chlamydia psittaci antigen in paraffin-embedded tissue sections. | a murine monoclonal antibody prepared against an ovine abortion isolate of chlamydia psittaci (a22/teramo) revealed specific binding to a 57 kda chlamydial antigen in immunoblotting studies. the monoclonal antibody was able to detect intracytoplasmic chlamydial inclusions and scattered elementary bodies in infected mccoy cell culture, and on formalin-fixed paraffin-embedded tissue sections both from experimentally infected mice and from fetal membranes of cases of ovine enzootic abortion. | 1992 | 1465516 |
| [the ultrastructure of a new species of chlamydia--chlamydia pneumoniae]. | the dynamic study of a new chlamydia species, c. pneumoniae (strain twar, isolate tw-480), inoculated into the monolayer culture of cells l-929 was made 24, 48, 72 and 96 hours after inoculation. when compared with c. trachomatis and c. psittaci, c. pneumoniae were found to stand between these two species with respect to the morphology of their intracytoplasmic microcolonies (inclusions): they were round, almost bubble-like, but more densely packed with chlamydiae, surrounded by an undulate memb ... | 1992 | 1466164 |
| specificity of the microimmunofluorescence assay for the serodiagnosis of chlamydia pneumoniae infections. | chlamydia pneumoniae infections are mostly confirmed using an indirect microimmunofluorescence test for which potential cross-reactions between antigens from different chlamydial species are not well documented. using this assay, 928 sera (507 subjects) submitted for chlamydia pneumoniae serology were tested for specific igm and igg to this bacteria using the tw-183 antigen. igm and igg reactivities to chlamydia trachomatis serotypes c, d, e, and l2 and chlamydia psittaci strain 6bc antigens wer ... | 1992 | 1477792 |
| chlamydial infections of the heart. | chlamydiae are common human pathogens, causing a broad spectrum of infectious diseases. chlamydial infections involving the heart have been described in numerous previous reports. these organisms are documented to cause endocarditis, myocarditis and pericarditis. furthermore, chlamydia pneumoniae, the recently discovered respiratory pathogen, has also been implicated in coronary artery disease. for the first time the literature on involvement of the heart in chlamydial infections is reviewed. in ... | 1992 | 1486883 |
| use of hep-2 cells for improved isolation and passage of chlamydia pneumoniae. | chlamydia pneumoniae has proved to be difficult to isolate and propagate in cell culture. we compared the growth of three strains of c. pneumoniae, tw-183 and two clinical isolates from brooklyn, n.y., in five cell lines, including hela 229, mccoy, hl, hep-2, and hted, an immortalized human tracheal cell line. hep-2 was the most sensitive cell line tested. when 10-fold dilutions of three c. pneumoniae strains at known titers were inoculated into the different cell lines, the mean number of inclu ... | 1992 | 1500500 |
| seroepidemiology of chlamydia pneumoniae twar infection in seattle families, 1966-1979. | incidence rates of chlamydia pneumoniae infection and information on reinfection and transmission within families were obtained by serologic study of serum samples from prospective family studies conducted 1966-1979. specimens (n = 3671) from 343 subjects in 68 families were tested for twar antibody using the microimmunofluorescence assay. acute infection was defined as a fourfold rise in antibody titer between consecutive specimens. sixty-four episodes of infection were identified in 58 persons ... | 1992 | 1500751 |
| [bilateral pneumonia caused by chlamydia psittaci. description of a clinical case]. | a mortal case of atypical pneumonia due to chlamydia psittaci with acute respiratory distress, septic shock and multiple organ failure is described. infection has been caused by an ill parrot imported clandestinely. antibody titration with the immunofluorescence technique allowed diagnosis. | 1992 | 1508365 |
| hilar lymphadenopathy associated with chlamydia pneumoniae infection. | a 27-year-old man with an acute chlamydia pneumoniae infection and hilar lymph node involvement is reported. further investigation gave no evidence of sarcoidosis or other known cause of enlarged hilar nodes. serological tests proved an acute infection with c. pneumoniae thus, an infection with c. pneumoniae may lead to enlargement of the hilar lymph nodes, and infection with this microorganism should be included in the differential diagnostic considerations in patients with hilar lymphadenopath ... | 1992 | 1509245 |
| [infection by avian chlamydiosis in breeding pigeons in new caledonia]. | an epidemiological survey on avian chlamydiosis, carried out by serological probing in 8 pigeon breeders representative of new caledonian livestock, combined with bacteriological research on pigeon organs and droppings was set up in new caledonia in order to determine the prevalence rate of this infection and to adapt sanitary regulations concerning pigeon imports. all sera collected (230) were analysed by complement fixation test (cft). the organs were diluted in sucrose solution, then stored f ... | 1992 | 1510340 |
| detection of chlamydia pneumoniae in coronary arterial fatty streaks and atheromatous plaques. | there is serological evidence that a recently described new strain of chlamydia, namely chlamydia pneumoniae twar, is associated with coronary artery disease. this study describes the detection of twar organisms in the coronary arterial fatty streaks and atheromatous plaques in 7 autopsy cases. twar-like organisms were observed ultrastructurally in the lipid-rich core area of fibrolipid plaques and in intimal smooth-muscle cells. in 5 cases, immunoperoxidase staining showed positive reactions to ... | 1992 | 1519134 |
| isolation of chlamydia pneumoniae from the maxillary sinus of a patient with purulent sinusitis. | | 1992 | 1520822 |
| monoclonal antibody based capture elisa/elifa for the detection of chlamydia psittaci in veterinary clinical specimens. | a capture elisa/elifa system based on monoclonal capture and biotinylated monoclonal detection antibody with specificity for an epitope on chlamydial lipopolysaccharide (lps) is described. the assay is fast, specific and detects a minimum dose of approximately 2000 chlamydial particles (c. psittaci). unlike other commercially available test kits, it comprises a proteinase k treatment to exclude non-specific binding of antibodies to fc receptors. in contrast to the sophisticated and cumbersome is ... | 1992 | 1520968 |
| ovine chlamydiosis in an abattoir worker. | the strain of chlamydia psittaci causing enzootic abortion in ewes (the eae strain) may cause serious infection in pregnant women, often resulting in hepatic and renal dysfunction, disseminated intravascular coagulation and fetal loss. the first case of such an infection in an abattoir worker is described and the possibility of human-to-human transmission considered. direct handling of sheep or their products of conception can usually be established but this is not always so. there is much still ... | 1992 | 1522332 |
| coxiella burneti endocarditis in a patient with positive chlamydial serology. | a 41-year-old man who habitually slept in a car park presented with a culture-negative endocarditis. serological tests indicated infection with both coxiella burneti and chlamydia psittaci. he was treated with doxycycline and clindamycin and required aortic valve replacement. culture of the excised value for both agents was negative but electron microscopy was suggestive of coxiella endocarditis. | 1992 | 1522334 |
| the epidemiology and significance of chlamydia pneumoniae. | chlamydia pneumoniae has recently been found to be a new chlamydial species transmitted directly from man to man, obviously through the respiratory tract. it is the commonest chlamydia of mankind but fortunately the overwhelming majority of infections are mild, it has been estimated that 10% of all pneumonias are caused by this species. since the most important chlamydial infections are chronic in nature, the question of possible chronic c. pneumoniae infections is of paramount importance. apart ... | 1992 | 1522335 |
| prevalence of antibodies to chlamydia pneumoniae in a belgian population. | to evaluate the prevalence of antibodies to chlamydia pneumoniae in a healthy adult belgian population a study group of 150 medical students was chosen. sera were collected in the period between march and october 1990 and assessed by the microimmunofluorescence test. sixty-one per cent were found to have igg antibodies to c. pneumoniae in a titre greater than or equal to 16, which showed evidence of past infection. twenty-one per cent had iga in a titre greater than or equal to 8. in none were a ... | 1992 | 1522343 |
| a comparison of the seroepidemiology of chlamydial infection in pigeon fanciers and farmers in the u.k. | a detailed comparative seroepidemiological study of antibody responses was performed in 271 pigeon fanciers and 100 farmers. overall 73% of pigeon fanciers had igg antibodies at a titre greater than or equal to 16 to chlamydia pneumoniae, 39% to chlamydia psittaci, and 6.6% to chlamydia trachomatis. the prevalence of chlamydial antibodies was significantly lower in the farmers at 47% for c. pneumoniae, 6% for c. psittaci, and 2% for c. trachomatis. both populations were exposed to complex microb ... | 1992 | 1522344 |
| a family outbreak of chlamydia pneumoniae infection. | chlamydia pneumoniae, a newly described chlamydia species, has been shown to be a cause of acute respiratory tract infection in both adults and children, but its role in human infection is still under investigation. here we present a family outbreak of c. pneumoniae infection where three members of a family presented with a 'flu-like illness' and acute upper respiratory tract infection which did not improve despite penicillin or septrin therapy. no history of exposure to birds, pets or animals w ... | 1992 | 1522345 |
| community-acquired pulmonary infection due to chlamydia in tropical queensland. | by using enzyme immunoassay and immunofluorescence antigen detection techniques on sputum specimens, four of 260 patients with pulmonary infection resident in tropical queensland were found to be infected with chlamydia. all four chlamydial infections were community-acquired and there was no history of close contact with birds by any of the four patients. one woman was deemed to be suffering with chlamydia pneumonia, while the role of the organism in the pathogenesis of respiratory disease in th ... | 1992 | 1523467 |
| detection of chlamydia pneumoniae by polymerase chain reaction. | while criteria for serodiagnosis of chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. to increase detection of this organism, c. pneumoniae-specific sequences were identified to permit amplification of c. pneumoniae by polymerase chain reaction (pcr). a cloned c. pneumoniae 474-bp psti fragment was shown by dot blot and southern hybridization to differentiate c. pneumoniae from the other chlamydia spp., react with all c. pneumoniae isolates tested ... | 1992 | 1537913 |
| l-arginine-dependent reactive nitrogen intermediates and the antimicrobial effect of activated human mononuclear phagocytes. | the l-arginine-dependent generation of reactive nitrogen intermediates (rni) has been identified as a key intracellular antimicrobial mechanism of activated mouse macrophages. to determine the role of this mechanism in the activity of human mononuclear phagocytes, monocyte-derived macrophages activated in vitro by interferon (ifn)-gamma and monocytes from patients receiving ifn-gamma as therapy were treated with ng-monomethyl-l-arginine (nma) or arginase. neither competitive inhibition of l-argi ... | 1992 | 1538156 |
| a prolonged outbreak of ornithosis in duck processors. | in 1985 an outbreak of ornithosis affected 13 of 80 (16%) workers in a duck-processing plant. new employees were three times more likely to become cases than established employees. the highest attack rate was in those on the production line. following the outbreak, an occupational health scheme was set up to monitor the health of new recruits to the company. serological evidence of recent infection was demonstrated in 18 of 37 (49%) new employees tested in the first 3 months of employment. five ... | 1992 | 1547838 |
| interspecies structural diversity among chlamydial genes encoding histone h1. | recently, a eukaryotic histone h1-like protein has been detected in chlamydia trachomatis serovar l2 [hackstadt et al., proc. natl. acad. sci. usa 88 (1991) 3937-3941; tao et al., j. bacteriol. 173 (1991) 2818-2822]. we have cloned the corresponding gene from c. trachomatis serovar j and the chlamydia psittaci strain mn. sequencing demonstrated absolute gene identity between the two c. trachomatis serovars l2 and j, but divergence in the c. psittaci strain mn. these differences resulted in alter ... | 1992 | 1551591 |
| brief report: chlamydia psittaci endocarditis diagnosed by blood culture. | | 1992 | 1557094 |
| antigenic and morphological differentiation of placental and intestinal isolates of chlamydia psittaci of ovine origin. | ewe placental and lamb intestinal isolates of chlamydia psittaci recovered from flocks affected with ovine enzootic abortion were examined by inclusion morphology, indirect immunofluorescence (iif) and immunoblot analysis. chlamydiae recovered from the faeces of sheep from two flocks free of clinical disease were also examined. in cell culture ovine abortion (oa) and intestinal isolates were distinguishable by inclusion development and morphology. similarly, in two-way iif tests with one week mo ... | 1992 | 1557895 |
| demonstration of chlamydia pneumoniae in water used for gargling. | | 1992 | 1563814 |
| seroprevalence of chlamydia pneumoniae infections in otolaryngeal diseases. | we studied the seroprevalence of antibodies to chlamydia pneumoniae, the third species of chlamydia, in patients with acute infections in otolaryngeal sites. the patients were divided into five diagnostic groups, i.e. sinusitis, otitis media, tonsillitis, laryngitis and bronchitis group. antibodies were measured by the microimmunofluorescent method. antibodies confirming past infection with c. pneumoniae were found 46.2 per cent of the patients in the diagnostic groups and 44.7 per cent of the s ... | 1992 | 1564376 |
| persistent infection with chlamydia pneumoniae following acute respiratory illness. | chlamydia pneumoniae is emerging as a significant cause of respiratory disease, including pneumonia and bronchitis, in humans. in this recently completed study of infection due to c. pneumoniae in patients presenting with pneumonia to suny health science center at brooklyn, we identified two individuals for whom cultures were positive on multiple occasions over a 1-year period. to determine the frequency of persistent respiratory infection with c. pneumoniae, follow-up specimens were obtained fr ... | 1992 | 1571425 |
| [twar infection is a common diagnosis in outpatient clinics]. | infections caused by chlamydia pneumoniae were first described in 1985. the infection can cause common cold, sore throat, hoarseness, cough, headache, fatigue and sometimes influenza-like illness. examination can indicate serous otitis media, sinusitis, laryngitis, bronchitis and pneumonia. the course can be long and relapsing. the recommended drugs for treatment are tetracycline or erythromycin for at least two weeks. five verified cases are described in the article, four of them with symptoms ... | 1992 | 1573935 |
| chlamydia pneumoniae, strain twar pneumonia. | chlamydia pneumoniae, strain twar is a newly recognized third species of chlamydia. it is an important cause of pneumonia, bronchitis, sinusitis, and other acute respiratory infections. it is a human pathogen transmitted by the respiratory route. successful antibiotic treatment with tetracycline or erythromycin requires high dosage and a prolonged course. | 1992 | 1580592 |
| age-related interference with chlamydia pneumoniae microimmunofluorescence serology due to circulating rheumatoid factor. | microimmunofluorescence (mif) serology is commonly used in the diagnosis of chlamydial infections. in the mif assay, chlamydia pneumoniae elementary bodies were used to detect c. pneumoniae immunoglobulin g (igg) and igm antibodies in paired serum samples from 286 patients with respiratory illnesses. in 69 patients, mif serology was compared with c. pneumoniae cultures. all c. pneumoniae cultures remained negative. however, 205 (71%) of 286 patients were c. pneumoniae antibody positive and 64 (2 ... | 1992 | 1583133 |
| regional differences of chlamydia pneumoniae as causative agent of pneumonia in sweden. | a retrospective serological study was performed on sera from 1982-83 and 1989 to investigate the incidence of chlamydia pneumoniae infection in hospital treated patients with pneumonia in orebro county. paired sera from 231 patients were available and 3 cases (1.3%) of c. pneumoniae infection were documented by microimmunofluorescence. the results differ from those reported by others implying regional differences of c. pneumoniae as causative agent of pneumonia. the findings from orebro county d ... | 1992 | 1589717 |
| effects of interferons beta or gamma on neopterin biosynthesis and tryptophan degradation by human alveolar macrophages in vitro: synergy with lipopolysaccharide. | interferons can induce neopterin biosynthesis and tryptophan degradation in monocytic cells. indoleamine 2,3-dioxygenase (ido), an inducible cellular enzyme, metabolizes tryptophan to n-formyl-l-kynurenine. tryptophan degradation has been linked to interferon-mediated inhibition of replication by intracellular pathogens and inhibition of cancer cell proliferation. we evaluated the ability of the recombinant human interferons beta ser and gamma to stimulate neopterin production and tryptophan deg ... | 1992 | 1591013 |
| chlamydiosis in a red-tailed hawk (buteo jamaicensis). | a red-tailed hawk (buteo jamaicensis) with signs of respiratory distress and diarrhea was captured in the manchac wildlife management area, louisiana (usa) and died the following day. at necropsy, the carcass was emaciated and there were splenomegaly, and fibrinous pericarditis, airsacculitis, and perihepatitis. microscopically, there were fibrinous pericarditis and airsacculitis, myocardial necrosis, necrotizing hepatitis, splenic necrosis with reticuloendothelial cell hyperplasia, interstitial ... | 1992 | 1602582 |
| chlamydia, smoking, and heart disease. | | 1992 | 1605436 |
| association of prior infection with chlamydia pneumoniae and angiographically demonstrated coronary artery disease. | to evaluate the association between prior infection with chlamydia pneumoniae, as measured by igg antibody, and coronary artery disease. | 1992 | 1608116 |
| chlamydia psittaci infection in horses: results of a prevalence survey and experimental challenge. | nasal and conjunctival swabs were obtained from 300 horses and chlamydia psittaci was isolated from 15 of them (5 per cent). eleven nasal swabs and six conjunctival swabs were positive on culture, but there was no association between the isolation of the organism and the presence of clinical ocular or respiratory disease. six ponies were challenged with an equine isolate of c psittaci into the eye, nasal cavity or bronchial tree. the organism could be isolated from nasal and conjunctival swabs t ... | 1992 | 1609475 |
| evaluation of a commercial solid-phase enzyme immunoassay for the detection of ovine chlamydia psittaci. | | 1992 | 1616986 |
| lumbosacral meningoradiculitis associated with chlamydia pneumoniae infection. | | 1992 | 1619428 |
| diagnosis of avian chlamydiosis: specificity of the modified giménez staining on smears and comparison of the sensitivity of isolation in eggs and three different cell cultures. | for the diagnosis of chlamydiosis in dead and live birds different methods were compared for their sensitivity and specificity. the specificity of the modified giménez staining and the direct immunofluorescence (dif) test for direct demonstration of chlamydia psittaci in organ, cloacal and/or conjunctival smears was examined. the sensitivity of the isolation of chlamydia psittaci in 6 days embryonated specific pathogen free (spf) chicken eggs, buffalo green monkey (bgm) cell line, mccoy cell lin ... | 1992 | 1621472 |
| acute glomerulonephritis following infection with chlamydia psittaci. | | 1992 | 1621687 |
| factors influencing the infectivity of chlamydia pneumoniae elementary bodies on hl cells. | the influence of variations in the ph, nacl concentration, temperature, and concentrations of calcium and magnesium ions on the survival of chlamydia pneumoniae elementary bodies (ebs) outside the host cells was investigated. the survival was determined after various incubation periods by counting the inclusion-forming units after c. pneumoniae was cultured for 72 h on monolayers of hl cells. the normal physiological conditions were restored prior to infecting the hl cells with c. pneumoniae. de ... | 1992 | 1624553 |
| [isolation of chlamydia pneumoniae and antibodies to the agent in patients with acute bronchitis]. | isolation of chlamydia pneumoniae and serologic tests were performed on 85 children and adults patients with acute bronchitis admitted to the kitasato institute hospital from january 1989 to may 1991. c. pneumoniae was isolated from sputa or tonsillar swabs in 11 of 57 (19%) patients examined. of these, 10 patients were tested serologically and 9 (90%) had 16 or higher titer of igg antibody. a total of 68 patients were tested serologically, and 17 (25%) revealed positive; 14 had a fourfold rise ... | 1992 | 1624841 |
| recovery of chlamydia pneumoniae, in six patients with otitis media with effusion. | six cases of otitis media with effusion associated with chlamydia pneumoniae, a currently recognized respiratory tract pathogen, are presented. the organism was isolated from the middle ear fluids and serological evidence confirmed it as the infectious agent. the study population is small; however, these reports suggest c. pneumoniae as a causative agent of middle ear diseases. | 1992 | 1624880 |