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ecological adaption analysis of the cotton aphid (aphis gossypii) in different phenotypes by transcriptome comparison.the cotton aphid, aphis gossypii glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. however, no transcriptome information is available for the cotton aphid and sequence data are scarce. therefore, we obtained transcriptome data.201324376660
prey species preference of the predator serangium parcesetosum sicard (col., coccinellidae) and its interaction with another natural enemy.this study aimed to determine serangium parcesetosum preference for different prey species and parasitized bemisia tabaci (genn.) (hom., aleyrodidae) by the parasitoid, eretmocerus mundus mercet (hym., aphelinidae). the results on s. parcesetosum preference by feeding on different prey species offered separately and together indicated that the predatory larvae and adults preferred significantly the cotton whitefly, b. tabaci and the castor bean whitefly, trialeurodes ricini (misra) (hom., aleyro ...200719070175
effects of rootstock and flushing on the incidence of three insects on 'clementine de nules' citrus trees.we evaluated the influence of six different citrus rootstocks on the incidence of the citrus leafminer, phyllocnistis citrella stainton (lepidoptera: gracillariidae), and the aphid species, aphis gossypii glover and a. spiraecola patch (hemiptera: aphididae), on 'clementine de nules' trees (citrus clementina hort. ex tan.). sampling was conducted during 2005 and 2006 in a grove of 3-yr-old trees in southern spain with six rootstocks arranged in a completely randomized block design. incidence (i. ...200819161697
control of silverleaf whitefly, cotton aphid and kanzawa spider mite with oil and extracts from seeds of sugar apple.development of alternative methods for pest management is needed with the increased concern for adverse effects of pesticides for human health and the environment. the main goal of our study was to test the oil from seeds of sugar apple (annona squamosa), an edible tropical fruit for pest control. the oil pressed out of seeds was as effective in controlling the silverleaf whitefly, bemisia argentifolii bellows & perring (homoptera: aleyrodidae), infesting leaves of tomato plants in greenhouse co ...200919768275
modeling temporal trends in aphid vector dispersal and cucumber mosaic virus epidemics in snap bean.cucumber mosaic virus (cmv) has become a major limiting factor in snap bean production in the great lakes region of north america, and epidemics have occurred more frequently since the soybean aphid, aphis glycines matsumura, was introduced. major aphid vectors of cmv epidemics were identified by statistically relating their temporal dispersal trends to the incidence of cmv. alates were monitored weekly using water pan traps in 74 snap bean fields in new york and pennsylvania from 2002 to 2006. ...200919825288
a genetic map of melon (cucumis melo l.) with rflp, rapd, isozyme, disease resistance and morphological markers.one hundred and ten markers were analysed for linkage in 218 f2 plants derived from two divergent cultivars ('védrantais' and 'songwhan charmi') of cucumis melo (l.). thirty-four rflps, 64 rapds, one isozyme, four disease resistance markers and one morphological marker were used to construct a genetic map spanning 14 linkage groups covering 1390 cm of the melon genome. rapd and rflp markers detected similar polymorphism levels. rflps were largely due to base substitutions rather than insertion/d ...199624162199
evidence of horizontal transfer of non-autonomous lep1 helitrons facilitated by host-parasite interactions.horizontal transfer (ht) of transposable elements has been recognized to be a major force driving genomic variation and biological innovation of eukaryotic organisms. however, the mechanisms of ht in eukaryotes remain poorly appreciated. the non-autonomous helitron family, lep1, has been found to be widespread in lepidopteran species, and showed little interspecific sequence similarity of acquired sequences at 3' end, which makes lep1 a good candidate for the study of ht. in this study, we descr ...201424874102
deceptive chemical signals induced by a plant virus attract insect vectors to inferior hosts.previous studies have shown that vector-borne pathogens can alter the phenotypes of their hosts and vectors in ways that influence the frequency and nature of interactions between them, with significant implications for the transmission and spread of disease. for insect-borne pathogens, host odors are particularly likely targets for manipulation, because both plant- and animal-feeding insects use volatile compounds derived from their hosts as key foraging cues. here, we document the effects of a ...201020133719
effects of vegetated field borders on arthropods in cotton fields in eastern north carolina.the influence, if any, of 5m wide, feral, herbaceous field borders on pest and beneficial arthropods in commercial cotton, gossypium hirsutum (l.) (malvales: malvaceae), fields was measured through a variety of sampling techniques over three years. in each year, 5 fields with managed, feral vegetation borders and five fields without such borders were examined. sampling was stratified from the field border or edge in each field in an attempt to elucidate any edge effects that might have occurred. ...200820345293
seasonal phenology and species composition of the aphid fauna in a northern crop production area.the species diversity of aphids and seasonal timing of their flight activity can have significant impacts on crop production, as aphid species differ in their ability to transmit plant viruses and flight timing affects virus epidemiology. the aim of the study was to characterise the species composition and phenology of aphid fauna in finland in one of the northernmost intensive crop production areas of the world (latitude 64°).201323967149
relation of aphicidal activity with cuticular degradation by beauveria bassiana sfb-205 supernatant incorporated with polyoxyethylene-(3)-isotridecyl ether.the application of beauveria bassiana sfb-205 supernatant incorporated with polyoxyethylene-(3)-isotridecyl ether (tde-3) significantly reduced the population of two species of aphids including cotton aphid, aphis gossypii, and green peach aphid, myzus persicae, much higher in cotton aphid, compared with supernatant incorporated with tween 80, which allows the relationship of aphicidal activity with the degradation of aphid cuticles to be determined. overall, the degradation of the cuticles indu ...201020372019
a novel biopesticide production: attagel-mediated precipitation of chitinase from beauveria bassiana sfb-205 supernatant for thermotolerance.insect killing fungi have high potential in controlling agriculturally harmful pest, but their slow progress and high variation in killing insect are major impediments to successful industrialization. the present work describes the use of supernatant from the liquid culture of beauveria bassiana sfb-205 to surmount this problem, particularly efficient production of thermotolerant chitinase, which is one of the major pathogenesis-related enzymes in the supernatant. the chitinase was precipitated ...201020445976
comparative analysis of the acyrthosiphon pisum genome and expressed sequence tag-based gene sets from other aphid species.to study gene repertoires and their evolution within aphids, we compared the complete genome sequence of acyrthosiphon pisum (reference gene set) and expressed sequence tag (est) data from three other species: myzus persicae, aphis gossypii and toxoptera citricida. we assembled ests, predicted coding sequences, and identified potential pairs of orthologues (reciprocical best hits) with a. pisum. pairwise comparisons show that a fraction of the genes evolve fast (high ratio of non-synonymous to s ...201020482638
correlation of the aphicidal activity of beauveria bassiana sfb-205 supernatant with enzymes.the supernatant of beauveria bassiana sfb-205 reduced the population of cotton aphid, aphis gossypii glover, with a dosage-dependent manner, which allowed a quality control (qc) factor to be determined for the evaluation of the supernatant as the first step of a development. enzymes were assumed as possible qc factors based on 1) the comparable aphicidal activity of the supernatant protein pellet to the raw supernatant, 2) the supernatant-induced degradation of the insect cuticles, observed by t ...201020965068
purification and characterization of a lectin with high hemagglutination property isolated from allium altaicum.a lectin was purified from the leaves of allium altaicum and corresponding gene was cloned. the lectin namely allium altaicum agglutinin (aaa) was ~24-ákda homodimeric protein and similar to a typical garlic leaf lectin. it was synthesized as 177 amino acid residues pre-proprotein, which consisted of 28 and 43 amino acid long n and c-terminal signal peptides, respectively. the plant expressed this protein more in scapes and flowers in comparison to the bulbs and leaves. hemagglutination activity ...201121732172
Rapid detection of vip1-type genes from Bacillus cereus and characterization of a novel vip binary toxin gene.A PCR-restriction fragment length polymorphism (PCR-RFLP) method for identifying vegetative insecticidal protein (vip) 1-type genes from Bacillus cereus was developed by designing specific primers based on the conserved regions of the genes to amplify vip1-type gene fragments. PCR products were digested with endonuclease AciI, and four known vip1-type genes were identified. Vip1Ac and vip1Aa-type genes appeared in 17 of 26 B. cereus strains. A novel vip1-type gene, vip1Ac1, was identified from B ...201122092859
bacterial communities of two parthenogenetic aphid species cocolonizing two host plants across the hawaiian islands.aphids (hemiptera: aphididae) have been the focus of several studies with respect to their interactions with inherited symbionts, but bacterial communities of most aphid species are still poorly characterized. in this research, we used bar-coded pyrosequencing to characterize bacterial communities in aphids. specifically, we examined the diversity of bacteria in two obligately parthenogenetic aphid species (the melon aphid, aphis gossypii, and the cardamom aphid, pentalonia caladii) cocolonizing ...201121965398
transgenic bt cotton does not disrupt the top-down forces regulating the cotton aphid in central china.top-down force is referred to arthropod pest management delivered by the organisms from higher trophic levels. in the context of prevalent adoption of transgenic bt crops that produce insecticidal cry proteins derived from bacillus thuringiensis (bt), it still remains elusive whether the top-down forces are affected by the insect-resistant traits that introduced into the bt crops. we explored how bt cotton affect the strength of top-down forces via arthropod natural enemies in regulating a non-t ...201627870914
evaluation of larvicidal and nymphicidal potential of plant extracts against anopheles subpictus grassi, culex tritaeniorhynchus giles and aphis gossypii glover.the acetone, chloroform, ethyl acetate, hexane and methanol extracts of peel and leaf extracts of citrus sinensis, ocimum canum, ocimum sanctum and rhinacanthus nasutus were tested against fourth instar larvae of malaria vector, anopheles subpictus grassi, japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae) and feeding deterrence to nymphs of cotton pest, aphis gossypii glover (homoptera: aphididae). the larval and nymph mortality were observed after 24 h of exposure ...200919050919
synthesis and biological evaluation of nitromethylene neonicotinoids based on the enhanced conjugation.the neonicotinoids with a nitroconjugated system had excellent bioactivity, which could rival imidacloprid, and has been previously reported. however, the photodegradation and hydrolysis of this series of neonicotinoids was very quick according to our further investigation, which cannot be developed as a pesticide further. the approach to further enhance the conjugation was tried not only to increase the bioactivities but also to improve the stability in water and in the sun. a substituted pheny ...201324180522
pest trade-offs in technology: reduced damage by caterpillars in bt cotton benefits aphids.the rapid adoption of genetically engineered (ge) plants that express insecticidal cry proteins derived from bacillus thuringiensis (bt) has raised concerns about their potential impact on non-target organisms. this includes the possibility that non-target herbivores develop into pests. although studies have now reported increased populations of non-target herbivores in bt cotton, the underlying mechanisms are not fully understood. we propose that lack of herbivore-induced secondary metabolites ...201323486438
[toxicity of vitex negundo extract to aphids and its co-toxicity with imidacloprid].in this paper, the toxicity of vitex negundo seed dichloromethane extract, imidacloprid, and omethoate to aphis citricola, aphis gossypii, and myzus persicae was determined by impregnation method, and the co-toxicity of the seed extract and imidacloprid to a. gossypii as well as the repellent effect of the extract and test insecticides to m. persicae were tested by topical application of capillary tube and by selection in culture dish, respectively. the results showed that the lc50 of v. negundo ...200919637611
novel mutations and expression changes of acetyl-coenzyme a carboxylase are associated with spirotetramat resistance in aphis gossypii glover.acetyl-coenzyme a carboxylase (acc) catalyses the carboxylation of acetyl-coenzyme a (acetyl-coa) to produce malonyl-coa during the de novo synthesis of fatty acids. spirotetramat, an inhibitor of acc, is widely used to control a range of sucking insects, including the aphis gossypii. in the present study, reverse transcription quantitative real-time pcr (rt-qpcr) results demonstrated that acc was significantly overexpressed in a laboratory-selected spirotetramat-resistant strain compared with t ...201728370744
separate and combined effects of mentha piperata and mentha pulegium essential oils and a pathogenic fungus lecanicillium muscarium against aphis gossypii (hemiptera: aphididae).in the present study, the toxicity of essential oils of mentha piperata l. and mentha pulegium l. and pathogenicity of lecanicillium muscarium (zare & gams) were studied in the melon aphid, aphis gossypii glover. analyses of the essential oils by gc-ms indicated limonene (27.28%), menthol (24.71%), menthone (14.01%), and carvol (8.46%) in the m. piperata essential oil and pulegone (73.44%), piperitenone (5.49%), decane (4.99%), and limonene (3.07%) in the essential oil of m. pulegium as the main ...201728334238
establishment of polyethylene-glycol-mediated protoplast transformation for lecanicillium lecanii and development of virulence-enhanced strains against aphis gossypii.lecanicillium lecanii has been developed as a biopesticide and used in biological control of several agricultural insects. to improve fungal virulence, an optimised polyethylene glycol (peg)-mediated protoplast transformation system was established for l. lecanii. pr1a-like cuticle-degrading protease gene (cdep1) from beauveria bassiana was transferred into l. lecanii, and its resulting activity against aphis gossypii was assessed.201626800336
the entomopathogenic fungal endophytes purpureocillium lilacinum (formerly paecilomyces lilacinus) and beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions.the effects of two entomopathogenic fungal endophytes, beauveria bassiana and purpureocillium lilacinum (formerly paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, aphis gossypii glover (homoptera:aphididae), through in planta feeding trials. in replicate greenhouse and field trials, cotton plants (gossypium hirsutum) were inoculated as seed treatments with two concentrations of b. bassiana or p. lilacinum conidia. positive colonization of cotton by the endophytes was c ...201425093505
spore density and viability of entomopathogenic fungal isolates from indonesia, and their virulence against aphis gossypii glover (homoptera: aphididae).the focus of this study was on quantifying fitness attributes, such as spore density and viability, and determining the virulence level against aphid (aphis gossypii) nymphs of isolates from the fungal species beauveria bassiana and metarhizium anisopliae. the fungal isolates were obtained from several insects, including plutella xylostella, hypothenemus hampei, bronstispa longissima, a. gossypii, tenebrio molitor, and leptocorisa acuta, that were collected from indonesian islands, such as sumat ...201024575186
spatial and temporal spread of citrus tristeza virus and its aphid vectors in the north western area of morocco.first report of citrus tristeza virus (ctv,closterovirus) in morocco datesback to 1961 in collections of citrus varieties. an exhaustive survey of citrus in the north of the country in 2009 revealed that ctv was spread all over the citrus production area. we attempted to evaluate the relative contribution of different aphid species in the spread of ctv disease in a citrus reticulata orchard at the loukkous region during 2 years (2012 and 2013). the overall ctv incidence estimated in the experime ...201625884375
a genetically novel, narrow-host-range isolate of cucumber mosaic virus (cmv) from rosemary.an isolate of cucumber mosaic virus (cmv), designated cmv-rom, was isolated from rosemary (rosmarinus officinalis) plants in several locations near avignon, france. laboratory studies showed that, unlike typical cmv isolates, cmv-rom has a particularly narrow host range. it could be transmitted by aphids aphis gossypii and myzus persicae, but with low efficacy compared to a typical cmv isolate. phylogenetic analysis of the nucleotide sequences of the cmv-rom genomic rnas shows that this isolate ...201627138549
the effect of chrysoperla carnea (neuroptera: chrysopidae) and adalia bipunctata (coleoptera: coccinellidae) on the spread of cucumber mosaic virus (cmv) by aphis gossypii (hemiptera: aphididae).the effects of two aphidophagous predators, the larvae of chrysoperla carnea and adults of adalia bipunctata, on the spread of cucumber mosaic virus (cmv) transmitted in a non-persistent manner by the cotton aphid aphis gossypii were studied under semi-field conditions. natural enemies and aphids were released inside insect-proof cages (1 m × 1 m × 1 m) with a central cmv-infected cucumber plant surrounded by 48 healthy cucumber seedlings, and the spatiotemporal dynamics of the virus and vector ...201525208589
presence of the aphid, chaetosiphon fragaefolii, on strawberry in argentina.seasonal abundance of the strawberry aphid complex under different agronomic practices in the outskirts of la plata, argentina was studied on strawberry, fragaria x ananassa duchesne (rosales: rosaceae). aphid densities were low in strawberry fields in which insecticides and fungicides were used. in addition to aphis gossypii, aphis fabae, mysus persicae and macrosiphum euphorbiae, the aphid, chaetosiphon fragaefolii (cockerell) (homoptera: aphididae), was recorded for the first time in this hor ...201020569141
identification and validation of reference genes for the normalization of gene expression data in qrt-pcr analysis in aphis gossypii (hemiptera: aphididae).to obtain accurate and reliable results from quantitative real-time reverse transcription polymerase chain reaction (qrt-pcr) analysis, it is necessary to select suitable reference genes as standards for normalizing target gene expression data. qrt-pcr is a popular analytical methodology for studying gene expression and it has been used widely in studies of aphis gossypii glover in recent years. however, there is absence of study on the stability of the expression of reference genes in a. gossyp ...201628076279
a type-iii insect geranylgeranyl diphosphate synthase with a novel catalytic property.geranylgeranyl diphosphate synthase (ggpp synthase or ggpps) is among the short-chain prenyltransferases, catalyzing the formation of the acyclic precursor ggpp for the biosynthesis of a variety of isoprenoids. ggppss have been extensively studied in plants, eubacteria, archaebacteria, yeast and mammals, but up to now information about an insect ggpps is still scarce. here we cloned the cdna encoding an insect ggpps from the cotton aphid (designated as agggpps). agggpps had an open reading frame ...201424527738
how accurate is the phenotype? - an analysis of developmental noise in a cotton aphid clone.the accuracy by which phenotype can be reproduced by genotype potentially is important in determining the stability, environmental sensitivity, and evolvability of morphology and other phenotypic traits. because two sides of an individual represent independent development of the phenotype under identical genetic and environmental conditions, average body asymmetry (or "fluctuating asymmetry") can estimate the developmental instability of the population. the component of developmental instability ...200818294398
in vitro and in planta compatibility of insecticides and the endophytic entomopathogen, lecanicillium lecanii.in an attempt to clarify the potential role of endophytic fungi in integrated pest management, the compatibility of an endophytic isolate of lecanicillium lecanii (zimmermann) gams & zare (hyphomycetes) with nine insecticides used against aphis gossypii glover (homoptera : aphididae) was examined both in vitro over 14 days and in planta. in the laboratory, most insecticides partially or completely inhibited the germination of conidia and growth of hyphae in nutrient-rich conditions. endosulfan c ...201121424605
transfer of cry1ac and cry2a proteins from genetically engineered bt cotton to herbivores and predators.with the cultivation of bt cotton, the produced insecticidal cry proteins are ingested by herbivores and potentially transferred along the food chain to natural enemies, such as predators. in laboratory experiments with bollgard ii cotton, concentrations of cry1ac and cry2ab were measured in lepidoptera larvae (spodoptera littoralis, heliothis virescens), plant bugs (euschistus heros), aphids (aphis gossypii), whiteflies (bemisia tabaci), thrips (thrips tabaci, frankliniella occidentalis), and s ...201728374515
effect of methomyl and oxamyl soil applications on early control of nematodes and insects.methomyl is a widely used carbamate insecticide that has traditionally been applied as a foliar spray. more recently, methomyl has been labeled as a soil application via drip chemigation. not much is known about the insecticidal and nematicidal potential of soil-applied methomyl. methomyl soil applications were evaluated for their potential to control soil nematodes and foliar insect pests in a series of lab and greenhouse tests.201121438120
plant resistance to aphid feeding: behavioral, physiological, genetic and molecular cues regulate aphid host selection and feeding.aphids damage major world food and fiber crops through direct feeding and transmission of plant viruses. fortunately, the development of many aphid-resistant crop plants has provided both ecological and economic benefits to food production. plant characters governing aphid host selection often dictate eventual plant resistance or susceptibility to aphid herbivory, and these phenotypic characters have been successfully used to map aphid resistance genes. aphid resistance is often inherited as a d ...201424282145
[transmission of passion fruit woodiness virus by aphis gossypii (glover) (hemiptera: aphididae) and colonization of passion flower by the vector].the transmission of passion fruit woodiness virus (pwv) by aphis gossypii (glover) was evaluated. in two independent experiments, a. gossypii transmitted pwv to passion fruit plants at the rates of 75% and 100%, when eight and twelve viruliferous aphids were deposited by plant, respectively. at the end of the tests, nymphs of a. gossypii were observed in some of the passion fruit plants, suggesting that the aphid species was colonizing the plants. this seems to be the first report of passiflora ...200717352080
monitoring of aphid flight activities in seed potato crops in serbia.aphid flight activities in seed potato fields have been studied by the yellow water traps. it is a good method for monitoring aphids as vectors of viruses, but this study also showed it is a suitable method for insect-diversity research. during the four-year studies, over 11.500 specimens were collected and a total of 107 different taxa of aphids were identified. the most abundant species were polyphagous species, such as: acyrthosiphon pisum (haris), aphis fabae scopoli, aphis gossypii gloveran ...201324039529
population dynamics of three aphid species (hemiptera: aphididae) on four ipomoea spp. infected or noninfected with sweetpotato potyviruses.three aphid species, aphis gossypii glover and myzus persicae (sulzer) (efficient sweetpotato potyvirus vectors) and rhopalosiphum padi (l.) (an inefficient vector), are commonly found in sweet potato, ipomoea batatas (l.), in louisiana. field-grown sweet potatoes are naturally infected with several potyviruses: sweet potato feathery mottle virus, sweet potato virus g, and sweet potato virus 2. thus, these aphids commonly encounter virus-infected hosts. what is not known is how each of these aph ...201324020267
outcomes of co-infection by two potyviruses: implications for the evolution of manipulative strategies.recent studies have documented effects of plant viruses on host plants that appear to enhance transmission by insect vectors. but, almost no empirical work has explored the implications of such apparent manipulation for interactions among co-infecting pathogens. we examined single and mixed infections of two potyviruses, watermelon mosaic virus (wmv) and zucchini yellow mosaic virus (zymv), that frequently co-occur in cucurbitaceae populations and share the same aphid vectors. we found that zymv ...201323407835
plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients.cucurbita pepo plants were infected with zucchini yellow mosaic virus or maintained noninfected.aphis gossypii, which transmits the virus, lived longer and produced more offspring on infected than on noninfected plants. on infected plants, the intrinsic rate of natural increase fora. gossypii increased with time after inoculation. in a similar experiment, concentrations of phloem sap nutrients, including free amino acids, total protein, and sugars from infected and noninfected plants were compar ...199424242121
rapid assessment of insect fauna based on local knowledge: comparing ecological and ethnobiological methods.the rapid assessment of biodiversity making use of surveys of local knowledge has been successful for different biological taxa. however, there are no reports on the testing of such tools for sampling insect fauna. the present study aimed to evaluate the efficiency of different ethnobiological techniques for rapid sampling of insect fauna.201626932264
circadian rhythmicity of diazinon susceptibility, detoxifying enzymes, and energy reserves in aphis gossypii (hemiptera: aphididae).the daily susceptibility rhythm of the cotton aphid, aphis gossypii glover, to diazinon and the corresponding changes in the activity of three xenobiotic detoxifying enzymes-cytochrome p450 monooxygenases (p450), glutathione s-transferases (gsts), and esterases-were investigated. bioassays were conducted to estimate the median lethal doses (ld50) of diazinon at six different zeitgeber times (zt0, 4, 8, 12, 16, and 20) under constant light (ll) and lighting conditions of 16 h of light and 8 h of ...201627298427
aphids (hemiptera, aphididae) on ornamental plants in greenhouses in bulgaria.investigations on the species composition and host range of aphids on ornamental greenhouse plants in bulgaria was conducted over a period of five years, from 2008 to 2012. twenty greenhouses, growing ornamentals for landscaping, plant collections and other purposes were observed. they were located in the regions of sofia, plovdiv, smolyan, pavlikeni, varna and burgas. the total number of collected aphid samples was 279. their composition included 33 aphid species and one subspecies from 13 gene ...201324039530
comparative transcriptional analysis of the host-specialized aphids aphis gossypii (hemiptera: aphididae).host specialization is an ubiquitous character in aphid populations. many polyphagous aphid populations usually consist of several subpopulations that have strong fidelity to a specific host or a subset of host range. host specialization is an evolutional result of food habit of insects. however, genetic basis and molecular mechanism of host specialization are still unclear. in this study, we presented a comparative analysis on global gene expression profiles of three lineages of aphis gossypii ...201728334183
transcriptional responses of detoxification genes to four plant allelochemicals in aphis gossypii.aphis gossypii glover (hemiptera: aphididae) can damage a variety of agricultural crops, so it is very important for cotton aphids to evolve adaptive mechanisms to various allelochemicals from host plants. our results aim to provide a fundamental and rich resource for exploring aphid functional genes in a. gossypii. a transcriptome data set and five expression profile data sets of a. gossypii samples were analyzed by illumina sequencing platform. in total, 53,763,866 reads were assembled into 1, ...201728334129
ultrastructure of compatible and incompatible interactions in phloem sieve elements during the stylet penetration by cotton aphids in melon.resistance of the melon line tgr-1551 to the aphid aphis gossypii is based on preventing aphids from ingesting phloem sap. in electrical penetration graphs (epgs), this resistance has been characterized with a. gossypii showing unusually long phloem salivation periods (waveform e1) mostly followed by pathway activities (waveform c) or if followed by phloem ingestion (waveform e2), ingestion was not sustained for more than 10 minutes. stylectomy with aphids on susceptible and resistant plants was ...201728213963
nano-formulation enhances insecticidal activity of natural pyrethrins against aphis gossypii (hemiptera: aphididae) and retains their harmless effect to non-target predators.the insecticidal activity of a new nano-formulated natural pyrethrin was examined on the cotton aphid, aphis gossypii glover (hemiptera: aphididae), and the predators coccinella septempunctata l. (coleoptera: coccinellidae) and macrolophus pygmaeus rambur (hemiptera: miridae), in respect with the nano-scale potential to create more effective and environmentally responsible pesticides. pyrethrin was nano-formulated in two water-in-oil micro-emulsions based on safe biocompatible materials, i.e., l ...201728210947
identification of micrornas and their response to the stress of plant allelochemicals in aphis gossypii (hemiptera: aphididae).micrornas (mirnas) are a group of short non-coding rnas involved in the inhibition of protein translation or in mrna degradation. although the regulatory roles of mirnas in various biological processes have been investigated, there is as yet an absence of studies about the regulatory roles of mirnas involved in the metabolism of plant allelochemicals in insects.201728202045
multivariate approach to quantitative analysis of aphis gossypii glover (hemiptera: aphididae) and their natural enemy populations at different cotton spacings.the relationship between pests and natural enemies using multivariate analysis on cotton in different spacing has not been documented yet. using multivariate approaches is possible to optimize strategies to control aphis gossypii at different crop spacings because the possibility of a better use of the aphid sampling strategies as well as the conservation and release of its natural enemies. the aims of the study were (i) to characterize the temporal abundance data of aphids and its natural enemi ...201728181503
mass rearing and augmentative biological control evaluation of rhynocoris fuscipes (hemiptera: reduviidae) against multiple pests of cotton.rhynocoris fuscipes (fab.) (hemiptera: reduviidae) is a generalist predator of cotton pests and is commonly found inhabiting cotton-growing regions in southern india. with the goal of integrating this predator in standard management practices used against cotton pests on a commercial scale, (1) we developed a protocol for adult group rearing of this predator inside micro-environmental cages (mecs), and (2) we evaluated the biocontrol potential of mass-produced predators against cotton pests unde ...201728127861
identification of a new cotton disease caused by an atypical cotton leafroll dwarf virus in argentina.an outbreak of a new disease occurred in cotton (gossypium hirsutum) fields in northwest argentina starting in the 2009-10 growing season and is still spreading steadily. the characteristic symptoms of the disease included slight leaf rolling and a bushy phenotype in the upper part of the plant. in this study, we determined the complete nucleotide sequences of two independent virus genomes isolated from cotton blue disease (cbd)-resistant and -susceptible cotton varieties. this virus genome comp ...201728035870
mir-276 and mir-3016-modulated expression of acetyl-coa carboxylase accounts for spirotetramat resistance in aphis gossypii glover.acetyl-coenzyme a carboxylase (acetyl-coa carboxylase, acc) catalyses the carboxylation of acetyl-coa to produce malonyl-coa during de novo fatty acid synthesis. a laboratory-selected spirotetramat-resistant strain (sr) of cotton aphid was used in this study. rt-qpcr results demonstrated significant increases in the levels of acc transcript in the resistant strain compared to the susceptible strain. depletion of overexpressed acc transcripts by rnai also significantly enhanced the sensitivity of ...201627989834
nitenpyram, dinotefuran, and thiamethoxam used as seed treatments act as efficient controls against aphis gossypii via high residues in cotton leaves.the effects of eight neonicotinoid seed treatments against the cotton aphid aphis gossypii and its natural enemies in bt cotton fields were evaluated, and the concentrations of these neonicotinoids in cotton leaves and soil were also investigated. the results showed that all neonicotinoid seed treatments efficiently reduced a. gossypii populations throughout the cotton seedling stage. the percentages of curly leaf plants in all of the neonicotinoid seed treatments were below the threshold for ec ...201627960287
rapid evolution of symbiotic bacteria populations in spirotetramat-resistant aphis gossypii glover revealed by pyrosequencing.aphis gossypii is one of the most economically important insect pests for agriculture worldwide. aphids have developed symbiotic associations with bacterial species, which has led to morphological and molecular differences, such as body color and insecticide resistance. adults and 3rd instar nymphs of a laboratory-selected spirotetramat-resistant strain of cotton aphid presented 579-fold and 15-fold higher resistance to spirotetramat, respectively, than a susceptible strain (pan et al., 2015; pe ...201627788413
synthesis and biological activity of pyridazine amides, hydrazones and hydrazides.optimization studies on compounds initially designed to be herbicides led to the discovery of a series of [6-(3-pyridyl)pyridazin-3-yl]amides exhibiting aphicidal properties. systematic modifications of the amide moiety as well as the pyridine and pyridazine rings were carried out to determine if these changes could improve insecticidal potency.201727766752
sublethal and transgenerational effects of sulfoxaflor on the biological traits of the cotton aphid, aphis gossypii glover (hemiptera: aphididae).sulfoxaflor is a novel insecticide belonging to sulfoximine chemical class that can be used to control sap-feeding insects, notably aphis gossypii glover. in addition to its acute toxicity, it is also important to consider the possible sublethal effects when establishing a comprehensive understanding of the toxicity of a new insecticide. we assessed the effects of a low lethal concentration (lc25) of sulfoxaflor on biological parameters of a. gossypii adults (f0) and subsequent transgenerational ...201627670668
herbivore specificity and the chemical basis of plant-plant communication in baccharis salicifolia (asteraceae).it is well known that plant damage by leaf-chewing herbivores can induce resistance in neighbouring plants. it is unknown whether such communication occurs in response to sap-feeding herbivores, whether communication is specific to herbivore identity, and the chemical basis of communication, including specificity. we carried out glasshouse experiments using the california-native shrub baccharis salicifolia and two ecologically distinct aphid species (one a dietary generalist and the other a spec ...201627597176
cycloxaprid: a novel cis-nitromethylene neonicotinoid insecticide to control imidacloprid-resistant cotton aphid (aphis gossypii).imidacloprid is a nicotinic acetylcholine receptor (nachr) agonist with potent insecticidal activity. however, resistance to imidacloprid is a significant threat and has been identified in several pest species. cycloxaprid with cis-configuration is a novel neonicotinoid insecticide, which shows high activity against imidacloprid-resistant pests. the lc50 of imidacloprid against the resistant aphis gossypii was 14.33mgl(-1) while it was only 0.70mgl(-1) for the susceptible population, giving a re ...201627521919
synthesis and biological activity of a new class of insecticides: the n-(5-aryl-1,3,4-thiadiazol-2-yl)amides.optimization studies on a high-throughput screening (hts) hit led to the discovery of a series of n-(6-arylpyridazin-3-yl)amides with insecticidal activity. it was hypothesized that the isosteric replacement of the pyridazine ring with a 1,3,4-thiadiazole ring could lead to more potent biological activity and/or a broader sap-feeding pest spectrum. the resulting n-(5-aryl-1,3,4-thiadiazol-2-yl)amides were explored as a new class of insecticides.201727414747
presence of native prey does not divert predation on exotic pests by harmonia axyridis in its indigenous range.in china, two invasive pests, bemisia tabaci meam1 (gennadius) and frankliniella occidentalis (pergande), often co-occur with the native pest, aphis gossypii (glover), on plants of malvaceae and cucurbitaceae. all three are preyed on by the native ladybird, harmonia axyridis (pallas); however, the native predator might be expected to prefer native prey to the exotic ones due to a shared evolutionary past. in order to clarify whether the presence of native prey affected the consumption of these t ...201627391468
studies toward understanding the sar around the sulfoximine moiety of the sap-feeding insecticide sulfoxaflor.the discovery of sulfoxaflor (isoclast™ active) stemmed from a novel scaffold-based approach toward identifying bioactive molecules. it exhibits broad-spectrum control of many sap-feeding insect pests, including aphids, whiteflies, hoppers and lygus. systematic modifications of the substituents flanking each side of the sulfoximine moiety were carried out to determine whether these changes would improve potency.201727367042
insight into the durability of plant resistance to aphids from a demo-genetic study of aphis gossypii in melon crops.resistance breakdown has been observed following the deployment of plant cultivars resistant to pests. assessing the durability of a resistance requires long-term experiments at least at a regional scale. we collected such data for melon resistance conferred by the vat gene cluster to melon aphids. we examined landscape-level populations of aphis gossypii collected in 2004-2015, from melon-producing regions with and without the deployment of vat resistance and with different climates. we conduct ...201627330552
alatae production and population increase of aphid vectors on virus-infected host plants.zucchini yellow mosaic virus (zymv) and aphis gossypii glover are two components of a recently identified plant-parasite system that provides an excellent opportunity to study interrelations between a virus and a vector that share the same host, but have no direct physiological interaction. in a field experiment we documented numbers of alate and apterous a. gossypii on healthy cucurbita pepo and on plants inoculated with virus 0, 7, 14, and 21 days before aphid infestation. when plants were ino ...199228311813
interspecific associations between cycloneda sanguinea and two aphid species (aphis gossypii and hyadaphis foeniculi) in sole-crop and fennel-cotton intercropping systems.aphids cause significant damage to crop plants. studies regarding predator-prey relationships in fennel (foeniculum vulgare mill.) and cotton (gossypium hirsutum l.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. this study evaluated the association among hyadaphis foeniculi (passerini) (hemiptera: aphididae), aphis gossypii glover (hemiptera: aphididae) and cycloneda sanguinea (l.) ...201526241862
carboxylesterase-mediated insecticide resistance: quantitative increase induces broader metabolic resistance than qualitative change.carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (op) insecticides. carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (g/a151d) or from tryptophan to leucine at the 271 site (w271l), follo ...201526047115
identification and expression profiling of odorant binding proteins and chemosensory proteins between two wingless morphs and a winged morph of the cotton aphid aphis gossypii glover.insects interact with their environment and respond to the changes in host plant conditions using semiochemicals. such ecological interactions are facilitated by the olfactory sensilla and the use of olfactory recognition proteins. the cotton aphid aphis gossypii can change its phenotype in response to ecological conditions. they reproduce mainly as wingless asexual morphs but develop wings to find mates or new plant hosts under the influence of environmental factors such as temperature, plant n ...201324073197
molecular cloning and characterization of a prenyltransferase from the cotton aphid, aphis gossypii.prenyltransferases play a key role in isoprenoid biosynthesis. here, a cdna encoding a prenyltransferase was isolated from the cotton aphid, aphis gossypii, which consists of 1354 nucleotides and encodes a protein of 394 amino acids (agipps). subsequent sequencing of agipps genomic dna resulted in one 3138-bp sequence. southern blotting analysis indicated that only a single ipps gene exists in the cotton aphid. real-time quantitative pcr showed that agipps transcripts were mainly present at the ...201020510361
the influence of natural enemies on wing induction in aphis fabae and megoura viciae (hemiptera: aphididae).previous studies have shown that the aphid species, aphis fabae scopoli and megoura viciae buckton, do not produce winged offspring in the presence of natural enemies, in contrast to results for the pea aphid (acyrthosiphon pisum (harris)) and the cotton aphid (aphis gossypii glover); but these studies did not involve exposing aphids directly to natural enemies. we exposed colonies of both a. fabae and m. viciae to foraging lacewing (chrysoperla carnea (stephens)) larvae and found that the preda ...200818076776
lethal, sublethal and transgenerational effects of the novel chiral neonicotinoid pesticide cycloxaprid on demographic and behavioral traits of aphis gossypii (hemiptera: aphididae).aphis gossypii glover (hemiptera: aphididae) is a key pest in cotton crops, notably owing to its increasing resistance to commonly used pesticides. such resistance prompts for the development of integrated pest management (ipm) programs that include novel pesticides being effective against the aphid. in the present study, we assessed lethal and sublethal effects of cycloxaprid, a novel chiral neonicotinoid pesticide developed in china, on a. gossypii. the lethal concentration at 50% (lc50 ) valu ...201627168374
effects of single and dual species herbivory on the behavioural responses of three thrips species to cotton seedlings.this study investigated the olfactory responses of three thrips species [frankliniella schultzei trybom, f. occidentalis pergrande and thrips tabaci lindeman (thysanoptera: thripidae)] to cotton seedlings [gossypium hirsutum l. (malvales: malvaceae)] simultaneously damaged by different combinations of herbivores. cotton seedlings were damaged by foliar feeding tetranychus urticae koch (trombidiforms: tetranychidae), helicoverpa armigera hübner (lepidoptera: noctuidae), aphis gossypii glover (hem ...201627029603
cytochrome p450 cyp6da2 regulated by cap 'n'collar isoform c (cncc) is associated with gossypol tolerance in aphis gossypii glover.cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. the survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome p450. gossypol has an antibiotic effect on aphis gossypii, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. the fecundity of the cotton aphids exposed t ...201627005728
the distribution and host shifts of cotton-melon aphids in northern china.aphis gossypii glover (hemiptera: aphididae) is a serious pest of cotton in northern china. a microsatellite analysis was used to characterize the genetic structure of a. gossypii populations from different geographic, host plant, and seasonal populations in 2014. among 906 individuals, 507 multilocus genotypes were identified, with genotypic richness values of 0.07-1.00 for the populations. we observed moderate levels of genetic differentiation among geographic populations (fst = 0.103; 95% con ...201627002824
effects of spirotetramat treatments on fecundity and carboxylesterase expression of aphis gossypii glover.spirotetramat is a novel tetramic acid-based insecticide, belonging to keto-enol pesticide family, with a novel mode of action; it interferes with lipid biosynthesis. its insecticide activity against various agricultural pest insects have been demonstrated (e.g. on myzus persicae, bemisia tabaci and tetranychus urticae). however, information available is currently limited on the efficacy of spirotetramat on the cotton aphid, aphis gossypii, a key cotton pest worldwide. we assessed the spirotetra ...201626898726
nbs-lrr-mediated resistance triggered by aphids: viruses do not adapt; aphids adapt via different mechanisms.aphids are serious pest on crops. by probing with their stylets, they interact with the plant, they vector viruses and when they reach the phloem they start a continuous ingestion. many plant resistances to aphids have been identified, several have been deployed. however, some resistances breaking down have been observed. in the melon, a gene that confers resistance to aphids has been deployed in some melon-producing areas, and aphid colony development on vat-carrying plants has been observed in ...201626801763
multiple mutations on the second acetylcholinesterase gene associated with dimethoate resistance in the melon aphid, aphis gossypii (hemiptera: aphididae).the melon aphid, aphis gossypii glover (hemiptera: aphididae), is an important cosmopolitan and extremely polyphagous species capable of causing direct and indirect damage to various crops. insecticide resistance in melon aphids is of particular concern. to determine the basis of resistance, organophosphate (op)-resistant strains of a. gossypii were obtained by continuous selection with dimethoate in the laboratory, and resistance mechanisms were investigated along with susceptible strains. thre ...201626797869
over-expression of cyp6a2 is associated with spirotetramat resistance and cross-resistance in the resistant strain of aphis gossypii glover.a laboratory-selected spirotetramat-resistant strain (sr) of cotton aphid developed 579-fold and 15-fold resistance to spirotetramat in adult aphids and 3rd instar nymphs, respectively, compared with a susceptible strain (ss) [26]. the sr strain developed high-level cross-resistance to alpha-cypermethrin and bifenthrin and very low or no cross-resistance to the other tested insecticides. synergist piperonyl butoxide (pbo) dramatically increased the toxicity of spirotetramat and alpha-cypermethri ...201626778436
an attractant of the aphidophagous gall midge aphidoletes aphidimyza from honeydew of aphis gossypii.many natural enemies of insects use honeydew as a volatile cue to locate hosts or prey, as an oviposition stimulant, and as an arrestant for foraging. the aphidophagous gall midge aphidoletes aphidimyza (rondani) (diptera: cecidomyiidae) has predacious larval stages and can be used to control aphid populations, especially in greenhouses. previous studies have shown that the honeydew, excreted by the aphid myzus persicae, attracts a. aphidimyza, but the crucial attractants have not been identifie ...201626757909
identification of aphis gossypii glover (hemiptera: aphididae) biotypes from different host plants in north china.the cotton-melon aphid, aphis gossypii glover (hemiptera: aphididae), is a polyphagous species with a worldwide distribution and a variety of biotypes. north china is a traditional agricultural area with abundant winter and summer hosts of a. gossypii. while the life cycles of a. gossypii on different plants have been well studied, those of the biotypes of north china are still unclear.201626735973
diet-delivered dsrnas for juvenile hormone-binding protein and vacuolar atpase-h implied their potential in the management of the melon aphid (hemiptera: aphididae).rna interference is a sequence-specific gene silencing mechanism mediated by double-stranded rna (dsrna), which has been harnessed as a useful tool in devising novel insect pest management strategies for various pests such as melon aphid, aphis gossypii (glover). in the current study, we cloned and sequenced juvenile hormone-binding protein (jhbp) and vacuolar atpase subunit h (v-atpase-h) from a. gossypii. we also showed the effectiveness of diet-mediated delivery of dsrna for jhbp and v-atpase ...201626645766
frequency detection of imidacloprid resistance allele in aphis gossypii field populations by real-time pcr amplification of specific-allele (rtpasa).the aphis gossypii glover (hemiptera: aphididae) is one of the most serious pests worldwide, and imidacloprid has been widely used to control this insect pest. just like other classes of insecticides, the resistance to imidacloprid has been found in a. gossypii. an amino acid mutation (r81t) in the nicotinic acetylcholine receptor (nachr) beta1 subunit was detected in the imidacloprid-resistant a. gossypii collected from langfang (lf) and dezhou (dz) cities. to estimate the r81t mutation frequen ...201526615144
host range specificity of scymnus camptodromus (coleoptera: coccinellidae), a predator of hemlock woolly adelgid (hemiptera: adelgidae).scymnus (neopullus) camptodromus yu and liu (coleoptera: coccinellidae) was brought to the united states from china as a potential biological control agent for hemlock woolly adelgid (adelges tsugae annand) (hemiptera: adelgidae). scymnus camptodromus phenology is closely synchronized with that of a. tsugae and has several characteristics of a promising biological control agent. as a prerequisite to field release, s. camptodromus was evaluated for potential nontarget impacts. in host range studi ...201626612409
functional response of aphidoletes aphidimyza rondani (diptera: cecidomyiidae) to aphis gossypii glover (hemiptera: aphididae): effects of vermicompost and host plant cultivar.interactions between natural enemies and herbivores may be affected by application of fertilizers and different cultivars. we investigated the functional response of the predatory gall midge, aphidoletes aphidimyza rondani (diptera: cecidomyiidae) larvae to the nymphs of the melon aphid, aphis gossypii glover (hemiptera: aphididae), reared on two commonly grown cucumber cultivars in iran (khasib and karim) treated with different vermicompost/soil ratios (0:100, 10:90, 20:80, and 30:70%). based o ...201626563403
evaluation of the synergistic effect between ethyl formate and phospine for control of aphis gossypii (homoptera: aphididae).cotton aphid, aphis gossypii glover, is known as a quarantine pest that is hard to control with short periods of fumigation with phosphine (ph(3)) or low concentrations of ethyl formate. moreover, low-temperature fumigation with ethyl formate can lead to phototoxic damage of some perishable commodities. therefore, a laboratory study was conducted to evaluate the synergistic effect of mixing ethyl formate and ph(3) for the treatment of adults and nymphs of a. gossypii. combined toxicity was obser ...201626476554
economic threshold for cotton aphid (hemiptera: aphididae) on cotton in the southwestern united states.cotton aphid, aphis gossypii glover (hemiptera: aphididae), is a common pest of cotton throughout much of the world. in the united states, insecticide applications targeting cotton aphid in cotton are common in the mid-south, texas, and california. cotton aphid population dynamics data were collected from eight insecticide efficacy trials conducted in lubbock, tx, over a 4-yr period. among the field populations in the nontreated plots, the instantaneous rate of population growth averaged 0.56 ±  ...201526470321
spirotetramat resistance adaption analysis of aphis gossypii glover by transcriptomic survey.a resistant strain of the cotton aphid (sr) developed 441.26-fold and 11.97-fold resistance to spirotetramat for adult aphids and nymphs, respectively, compared with the susceptible (ss) strain. solexa sequencing technology was employed to identify differentially expressed genes (degs) in the spirotetramat-resistant cotton aphid. respective totals of 22,430,522 and 21,317,732 clean reads were obtained from sr and ss cdna libraries and assembled into 35,222 non-redundant (nr) consensus sequences. ...201526453233
mirna-mediated auxin signalling repression during vat-mediated aphid resistance in cucumis melo.resistance to aphis gossypii in melon is attributed to the presence of the single dominant r gene virus aphid transmission (vat), which is biologically expressed as antibiosis, antixenosis and tolerance. however, the mechanism of resistance is poorly understood at the molecular level. aphid-induced transcriptional changes, including differentially expressed mirna profiles that correspond to resistance interaction have been reported in melon. the potential regulatory roles of mirnas in vat-mediat ...201626437210
rna interference of odorant-binding protein 2 (obp2) of the cotton aphid, aphis gossypii (glover), resulted in altered electrophysiological responses.aphis gossypii (glover) (hemiptera: aphididae) is a highly invasive pest that feeds primarily on phloem resulting in severe economic loss to growers. a. gossypii has cosmopolitan distribution with broad host range, polyphenism, parthenogenetic mode of reproduction, vectoring abilities, and host alteration which has profound influence on its management. odorant-binding proteins (obps) in insects are involved in olfaction, playing a key role in orienting the insect for feeding or oviposition. rece ...201626432291
impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions.poplar-cotton agro-ecosystems are the main agricultural planting modes of cotton fields in china. with increasing acres devoted to transgenic insect-resistant poplar and transgenic insect-resistant cotton, studies examining the effects of transgenic plants on target and non-target insects become increasingly important. we systematically surveyed populations of both target pests and non-target insects for 4 different combinations of poplar-cotton eco-systems over 3 years. transgenic bt cotton str ...201526345739
multi-trophic consequences of plant genetic variation in sex and growth.there is growing evidence for the influence of plant intraspecific variation on associated multi-trophic communities, but the traits driving such effects are largely unknown. we conducted a field experiment with selected genetic lines of the dioecious shrub baceharis salicifolia to investigate the effects of plant growth rate (two-fold variation) and gender (males vs. females of the same growth rate) on above- and belowground insect and fungal associates. we documented variation in associate den ...201627197400
identification of top-down forces regulating cotton aphid population growth in transgenic bt cotton in central china.the cotton aphid aphis gossypii glover is the main aphid pest in cotton fields in the yangtze river valley cotton-planting zone (yrz) in central china. various natural enemies may attack the cotton aphid in bt cotton fields but no studies have identified potential specific top-down forces that could help manage this pest in the yrz in china. in order to identify possibilities for managing the cotton aphid, we monitored cotton aphid population dynamics and identified the effect of natural enemies ...201425170907
comparative transcriptional analysis of asexual and sexual morphs reveals possible mechanisms in reproductive polyphenism of the cotton aphid.aphids, the destructive insect pests in the agriculture, horticulture and forestry, are capable of reproducing asexually and sexually upon environmental change. however, the molecular basis of aphid reproductive mode switch remains an enigma. here we report a comparative analysis of differential gene expression profiling among parthenogenetic females, gynoparae and sexual females of the cotton aphid aphis gossypii, using the rna-seq approach with next-generation sequencing platforms, followed by ...201424915491
complete mitochondrial genome of aphis gossypii glover (hemiptera: aphididae).the complete mitochondrial genome of the cotton-melon aphid, aphis gossypii glover, was sequenced using a combination of high-throughput sequencing, traditional pcr amplification, and sanger sequencing. the genome is 15,869 bp in length, and contains 37 typical coding genes, one non-coding at-rich region, and a repeat region found exclusively in aphids. the base composition of the genome is a (45.4%), t (38.3%), c (10.4%), and g (5.9%). all protein coding genes start with a typical atn initiatio ...201624865902
gene expression profiling in winged and wingless cotton aphids, aphis gossypii (hemiptera: aphididae).while trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. in this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, aphis gossypii, using a tag-based digital gene expression (dge) approach. ultra high-throughput illumina sequencing generated 5.30 and 5.39 million raw tags, respectively, from winged and wingless a. gossypii dge libraries. we identified 1 ...201424644424
quantification of the pirimicarb resistance allele frequency in pooled cotton aphid (aphis gossypii glover) samples by taqman snp genotyping assay.pesticide resistance monitoring is a crucial part to achieving sustainable integrated pest management (ipm) in agricultural production systems. monitoring of resistance in arthropod populations is initially performed by bioassay, a method that detects a phenotypic response to pesticides. molecular diagnostic assays, offering speed and cost improvements, can be developed when the causative mutation for resistance has been identified. however, improvements to throughput are limited as genotyping m ...201424614533
pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids myzus persicae, aphis gossypii, and aulacorthum solani (hemiptera: aphididae).seeking new isolates of entomopathogenic fungi with greater virulence against greenhouse aphid pests than those currently registered in north america for control of these insects, single-dose screening assays of 44 selected fungal isolates and 4 commercially available strains were conducted against first-instar nymphs of myzus persicae and aphis gossypii. the assays identified a number of beauveria and metarhizium isolates with virulence equal to or greater than that of the commercial strains ag ...201424583227
why the aphid aphis spiraecola is more abundant on clementine tree than aphis gossypii?aphis spiraecola and aphis gossypii cause harmful damages on clementine tree orchards. weekly surveys measured the abundance of aphids (larvae, winged and wingless adults) as well as of auxiliary insects and parameters of energy metabolism. correlatively, soluble carbohydrates, total free amino acids, free proline and condensed tannins were quantified in control and infested leaves. both aphid species showed parallel temporal variations, but a. spiraecola was consistently more abundant regardles ...201424581807
identification of differentially expressed genes related to aphid resistance in cucumber (cucumis sativus l.).cucumber, a very important vegetable crop worldwide, is easily damaged by pests. aphids (aphis gossypii glover) are among the most serious pests in cucumber production and often cause severe loss of yield and make fruit quality get worse. identifying genes that render cucumbers resistant to aphid-induced damage and breeding aphid-resistant cucumber varieties have become the most promising control strategies. in this study, a illumina genome analyzer platform was applied to monitor changes in gen ...201525959296
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