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mycotic aneurysm caused by streptococcus constellatus subsp. constellatus.an infected mycotic aneurysm due to streptococcus constellatus subsp. constellatus has not previously been reported. we report on this condition in an 87-year-old woman who had aggravating abdominal pain and a large fusiform aneurysm over the thoracic-abdominal aorta with mural thrombus. isolates from two sets of blood cultures and the debrided tissue were identified as s. constellatus subsp. constellatus by their biochemical reaction profiles, compatible 16s rrna gene sequencing results, and se ...200415071061
efficacy of antibiotics to strains of periodontopathogenic bacteria within a single species biofilm - an in vitro study.this study examined differences in the efficacy of antibiotics against a single strain of three periodontal pathogens grown in an artificial biofilm.200415086620
echocardiographic features of an unruptured mycotic aneurysm of the right aortic sinus of valsalva.this report describes a 25-year-old man with a pierced tongue in whom streptococcus constellatus endocarditis of the aortic valve developed. bacterial endocarditis in this patient was complicated by the development of a mycotic aneurysm of the right aortic sinus of valsalva. transthoracic and transesophageal echocardiographic studies were useful for the diagnosis of this rare lesion. findings were confirmed at operation.200415122191
the role of the capsule of the streptococcus milleri group in its pathogenicity.study of the pathogenicity of encapsulated strains of the streptococcus milleri group (smg) was performed by examination of the ability to cause subcutaneous abscesses in mice and by phagocytosis and phagocytic killing of human polymorphonuclear neutrophils (pmns) against the organisms. all 3 encapsulated isolates from patients with pneumonia or lung abscess induced abscesses in the mice; however, only 2 of 20 unencapsulated isolates from patients with lung abscess or thoracic empyema did so. th ...200415160304
clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis.the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy.200415367191
molecular analysis of age-related changes of streptococcus anginosus group and streptococcus mitis in saliva.the purpose of this study was to survey the prevalence of streptococcal species, especially streptococcus anginosus (which has been reported to be associated with cancer in the upper digestive tract), streptococcus constellatus, and streptococcus intermedius in the saliva of different age groups. a sequence analysis of 16s rdna was performed and dna quantified using real-time polymerase chain reaction. the s. anginosus level increased with age, whereas the levels of s. constellatus and s. interm ...200415491464
alkali-resistant bacteria in root canal systems.the aim of this study was to isolate and identify alkali-resistant bacteria from the dentin of infected root canals. bacteria from homogenized dentin powder made up from infected root canal walls from human teeth were cultured on buffer-enriched brain heart infusion agar supplemented with 4% sheep blood (bhi-blood agar), adjusted to ph 7.0, 9.0 or 10.0. incubation took place for 7 days at 37 degrees c in an anaerobic glove box. bacterial strains selected according to colony and morphology were s ...200415491465
surface free energy and bacterial retention to saliva-coated dental implant materials--an in vitro study.the aim of the present investigation was to compare the in vitro bacterial retention on saliva-coated implant materials (pure titanium grade 2 (cp-ti) and a titanium alloy (ti-6al-4v) surfaces), presenting similar surface roughness, and to assess the influence of physico-chemical surface properties of bacterial strain and implant materials on in vitro bacterial adherence. two bacterial strains (one hydrophilic strain and one hydrophobic strain) were used and the following were evaluated: bacteri ...200415555904
efficacy of antibiotics against periodontopathogenic bacteria within epithelial cells: an in vitro study.periodontopathogenic bacteria can invade and survive within epithelial cells, but susceptibility of intracellular infection to antibiotics used in periodontitis treatment has not been studied to date.200415562909
[mediastinitis and pleural empyema caused by streptococcus constellatus]. 200415574277
characterization of streptococcus constellatus strains recovered from a brain abscess and periodontal pockets in an immunocompromised patient.there have been a number of reports of brain abscesses suggesting an odontogenic etiology. however, no efforts have been made to compare brain abscess isolates with isolates from the oral cavity using highly discriminative methods. we report a brain abscess caused by streptococcus constellatus in an immunocompromised patient where oral infection (periodontitis) was suspected to be implicated.200415732877
phototargeting oral black-pigmented bacteria.we have found that broadband light (380 to 520 nm) rapidly and selectively kills oral black-pigmented bacteria (bpb) in pure cultures and in dental plaque samples obtained from human subjects with chronic periodontitis. we hypothesize that this killing effect is a result of light excitation of their endogenous porphyrins. cultures of prevotella intermedia and p. nigrescens were killed by 4.2 j/cm2, whereas p. melaninogenica required 21 j/cm2. exposure to light with a fluence of 42 j/cm2 produced ...200515793117
oral campylobacter species involved in extraoral abscess: a report of three cases.oral campylobacter species are rarely reported to cause extraoral infections. here we present three cases of extraoral abscess caused by an oral campylobacter sp. and a streptococcus sp. the campylobacter species were all isolated anaerobically and identified by sequencing analysis of the 16s rrna gene. the cases included a breast abscess caused by campylobacter rectus and a non-group a beta-hemolytic streptococcus in a patient with lymphoma, a liver abscess caused by campylobacter curvus and an ...200515872299
experimental abscess formation caused by human dental plaque.human dental plaque consists of a wide variety of microorganisms, some of which are believed to cause systemic infections, including abscesses, at various sites in the body. to confirm this hypothesis experimentally, we examined the abscess-forming ability of native dental plaque in mice, the microbial features of the infectious locus produced by the plaque, and the anti-phagocytic property of microbial isolates. aliquots of a suspension of supragingival dental plaque containing 6 x 10(6) colony ...200515905601
microarrays complement culture methods for identification of bacteria in endodontic infections.the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ...200515943771
chorioamnionitis associated with crohn's disease and azathioprine treatment: a case report.this paper reports a case of s. constellatus chorioamnionitis in a pregnant crohn's disease patient who was taking azathioprine. chorioamnionitis is a major cause of perinatal morbidity. azathioprine, an immunosuppressive antimetabolite, is widely used to treat inflammatory bowel disease. streptococcus constellatus is a gram-positive bacterium that has not previously been associated with chorioamnionitis. a high index of suspicion for chorioamnionitis and unusual pathogens should be maintained i ...200516014435
orbital abscess: visual loss following extraction of a tooth--case report.objective: it is the purpose of this article to alert the general practitioner to the severe consequences that may result from a tooth extraction, including the loss of vision, despite the use of antibiotics. conclusions: early and aggressive treatment is critical in obstructing the spread of infection toward the orbits, the eyes, and eventually the brain.200516182155
acute glomerulonephritis associated with streptococcus pyogenes with concomitant spread of streptococcus constellatus in four rural families.we studied history, renal histopathology and microbiology of an epidemic of acute glomerulonephritis associated with throat infections and uncommon culture results in four neighbour families. a 40-year-old man (index patient) was referred to a university hospital for dialysis and kidney biopsy due to a suspected acute glomerulonephritis. an acute tonsillitis had preceded the condition. penicillin treatment had been started four days before the discovery of renal failure. throat swabs were positi ...200516454159
rapid differentiation between members of the anginosus group and streptococcus dysgalactiae subsp. equisimilis within beta-hemolytic group c and g streptococci by pcr.based on a pair of primers developed initially for differentiating the anginosus group from other viridans streptococci, the pcr reported here can also differentiate between members of the anginosus group and streptococcus dysgalactiae subsp. equisimilis among beta-hemolytic group c and g streptococci. the resulting 742-bp pcr product was specific for members of the anginosus group, although a smaller, nonspecific product (361 bp) was generated from s. dysgalactiae subsp. equisimilis. restrictio ...200616672416
primary pulmonary botryomycosis diagnosed by fine needle aspiration cytology: a case report.primary pulmonary botryomycosis is an uncommon suppurative bacterial infection of the lung. cytologic findings by conventional methods and thin-layer preparations in1 case are presented.200616780031
surgical site infections in day surgery settings.because major complications are rare in day surgery, minor morbidities such as surgical site infections, which become evident only after patient discharge, have a major impact on the perceived quality of care. we performed an audit of such infections over a oneyear period.200616895493
microbiota of the dorsum of the tongue after plaque accumulation: an experimental study in humans.the purpose of the present investigation was to determine the effect of the absence of tongue hygiene on the microbiota of the dorsum of the tongue.200616945032
images in clinical medicine. elevated jugular venous pressure. 200616971712
tigecycline (gar-936) activity against streptococcus gallolyticus (bovis) and viridans group streptococci.viridans group streptococci including streptococcus gallolyticus (formerly s. bovis) represent serious invasive pathogens often associated with endocarditis or sepsis among immunocompromised or cancer patients. tigecycline (gar-936), the first clinically studied glycylcycline, has a potent gram-positive activity with a potential treatment option for these streptococcal infections. the studied collection (848 strains) included 100 isolates each of streptococcus anginosus, streptococcus constellat ...200716989974
[spontaneous bacterial peritonitis due to streptococcus constellatus]. 200617129558
[streptococcus constellatusas a causative agent of empyema. report of one case].streptococcus constellatus is a commensal microorganism in man but may cause infections in different locations. we report a 59 years old male with severe sequelae of a previous cerebrovascular accident that consulted in the emergency room for fever of 15 days of evolution. a right empyema was diagnosed. the bacteriological culture of the effusion disclosed the presence of streptococcus constellatus and two anaerobic strains (prevotella intermedia and fusobacterium urealyticus). the patient was t ...200617130992
streptococcus milleri group infection associated with digestive fistula in patients with vascular graft: report of seven cases and review.we described seven patients with streptococcus milleri group aortic (six patients) or vena cava (one patient) graft infection secondary to a vasculo-digestive fistula. time between vascular graft setting and first clinical signs varied from eight months to more than thirteen years. six patients had fever. three patients presented with recurrent fever for more than nine months and in two of these cases, delay before diagnosis was long because repeated blood cultures were sterile. three patients h ...200717565461
septic cavernous sinus thrombosis with bilateral secondary orbital infection.cavernous sinus thrombosis may occur as a secondary threatening condition to spread from local or distant foci of infection. we report a case where secondary retrograde spread occurred to both the ipsilateral and contralateral orbits with very different clinical manifestations. to our knowledge such spread is unreported. the case highlights the difficulty in recognising orbital collections when venous engorgement secondary to a cavernous sinus thrombosis coexists and demonstrates how the prognos ...200717613858
[necrotizing fasciitis complicating transthoracic biopsy].necrotizing fasciitis is a rapidly progressive and often fatal infection of the soft-tissue fascia deep to the skin but superficial to the muscles. we report a case of necrotizing fasciitis of the anterior chest wall complicating a percutaneous needle biopsy.200717978740
molecular and enzymatic characterization of betac-s lyase in streptococcus constellatus.streptococcus anginosus and streptococcus constellatus are frequently isolated from dental abscesses and other suppurative lesions. we previously reported that betac-s lyase from a strain of s. anginosus produced significantly more hydrogen sulfide than betac-s lyases from other streptococci. the purpose of this study was to establish the molecular and enzymatic features of the betac-s lyase in s. constellatus and to elucidate whether this unique capacity is common to many strains of s. constell ...200818402612
campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review.campylobacter species are frequently isolated from fecal specimens of patients with diarrheal illness. several campylobacter species are commonly isolated from the oral cavity. in contrast, campylobacter species are rarely isolated from extra-oro-intestinal abscesses. reported here are four cases of extra-oro-intestinal abscesses due to polymicrobial flora, including campylobacter species. the first case is a 35-year-old woman who was diagnosed with a brain abscess caused by c. gracilis, strepto ...200818488257
[streptococcus constellatus, epidural abscess and colovesical fistula]. 200818603391
a rare pathogen causing endocarditis: streptococcus constellatus. 200818676315
a polymicrobial perspective of pulmonary infections exposes an enigmatic pathogen in cystic fibrosis patients.lung disease is the leading cause of morbidity and mortality in cystic fibrosis (cf) patients. a modest number of bacterial pathogens have been correlated with pulmonary function decline; however, microbiological and molecular evidence suggests that cf airway infection is polymicrobial. to obtain a more complete assessment of the microbial community composition and dynamics, we undertook a longitudinal study by using culture-independent and microbiological approaches. in the process, we demonstr ...200818812504
identification and molecular analysis of betac-s lyase producing hydrogen sulfide in streptococcus intermedius.hydrogen sulfide (h(2)s) is a toxic gas that induces the modification and release of haemoglobin in erythrocytes; however, it also functions in methionine biosynthesis in bacteria. betac-s lyase, encoded by the lcd gene, is responsible for bacterial h(2)s production through the cleavage of l-cysteine. in this study, 26 of 29 crude extracts from reference and clinical strains of streptococcus intermedius produced h(2)s from l-cysteine. the capacities in those strains were not higher than those in ...200818927421
[treatment-refractory-dental-extraction-associated pyothorax involving infection by 2 species of oral originated bacteria requires surgical debridement by video assisted thoracoscopic surgery (vats)].cases of septic pulmonary embolism (spe) diagnosed clinically by ct after dental extraction rarely include verification of bacteria from the local infection site. we report the case of a 70-year-old man without background disease suffering severe pyothrax after dental extraction. we detected two species of oral bacteria from his pleural effusion. treatment was so difficult that it required surgical debridement by video assisted thoracoscopic surgery (vats), even after the appropriate administrat ...200818975591
immunobiological activities of bacteria isolated from the root canals of postendodontic teeth with persistent periapical lesions.although intraradicular bacteria are widely considered to be the primary etiological agents of periapical lesions, the immunobiological properties of the species in question are not adequately known. the aim of this study, therefore, was to investigate the pathobiological properties of the 10 most frequently isolated endodontopathic bacterial species. using cellular components of the pathogens obtained by sonic extraction, we have investigated their ability to induce monocyte migration, interleu ...199219186419
evaluation of genotypic and phenotypic methods for differentiation of the members of the anginosus group streptococci.the terminology and classification of the anginosus group streptococci has been inconsistent. we tested the utility of 16s rrna gene and tuf gene sequencing and conventional biochemical tests for the reliable differentiation of the anginosus group streptococci. biochemical testing included rapid id 32 strep, api strep, fluo-card milleri, wee-tabs, and lancefield antigen typing. altogether, 61 anginosus group isolates from skin and soft tissue infections and four reference strains were included. ...200919495818
primary omental abscess caused by streptococcus constellatus: a case report.the presentation of primary abscess of the greater omentum is similar to other acute abdominal disorders. it is a rarely reported phenomenon, and correct diagnosis is typically made during abdominal exploration.201019821706
streptococcus constellatus bacteremia causing septic shock following tooth extraction: a case report.organisms of the streptococcus milleri group consists of streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus. though they are commonly associated with abscesses, bacteremia with subsequent septic shock and mortality is infrequent.200919829816
[bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008].bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ...200919860320
direct 16s rrna gene sequencing of polymicrobial culture-negative samples with analysis of mixed chromatograms.two cases involving polymicrobial culture-negative samples were investigated by 16s rrna gene sequencing, with analysis of mixed chromatograms. fusobacterium necrophorum, prevotella intermedia and streptococcus constellatus were identified from pleural fluid in a patient with lemierre's syndrome and neisseria meningitidis and escherichia coli were identified from a petechia in a patient with meningococcal disease.201020035024
mckay agar enables routine quantification of the 'streptococcus milleri' group in cystic fibrosis patients.the 'streptococcus milleri' group (smg) has recently been recognized as a contributor to bronchopulmonary disease in cystic fibrosis (cf). routine detection and quantification is limited by current cf microbiology protocols. mckay agar was developed previously for the semi-selective isolation of this group. here, mckay agar was validated against a panel of clinical smg isolates, which revealed improved smg recovery compared with columbia blood agar. the effectiveness of this medium was evaluated ...201020093379
development of real-time pcr assays for detection of the streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16s rrna genes.cystic fibrosis (cf) is a multiorgan disease, with the majority of mortalities resulting from pulmonary failure due to repeated pulmonary exacerbations. recently, members of the streptococcus anginosus group (s. anginosus, s. constellatus, and s. intermedius), herein referred to as the "streptococcus milleri group" (smg) have been implicated as important etiological pathogens contributing to pulmonary exacerbations in cf patients. this is partly due to better microbiological detection of the smg ...201020164275
contribution of streptococcus anginosus to infections caused by groups c and g streptococci, southern india.vellore, a region in southern india, has a high incidence of severe human infections with beta-hemolytic group c and g streptococci (gcgs). to determine the causative species in these infections, we conducted 16s rrna gene sequencing: streptococcus dysgalactiae subsp. equisimilis (81%) and s. anginosus (19%) were the causative organisms in the 2-year study period (2006-2007). we used pcr to detect the virulence-related emm gene; results showed that it was restricted to s. dysgalactieae subsp. eq ...201020350380
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents --special references to bacteria isolated between april 2008 and march 2009].bacteria isolated from infections in abdominal surgery during the period from april 2008 to march 2009 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 712 strains including 18 strains of candida spp. were isolated from 173 (80.5%) of 215 patients with surgical infections. three hundred and sixty-six strains were isolated from primary infections, and 346 strains were isolated from postoperative infections. from primary infections, anaero ...201020919496
an uncommon late complication of appendicitis.a 10-year-old boy presented with a 2.5-week history of right leg pain and limp. a right flank mass was noted by a parent on the day of presentation. the child's past medical history was remarkable for perforated appendicitis treated with an interval laparoscopic appendectomy 2 years before this presentation. abdominal and pelvic computed tomography revealed a retroperitoneal mass with calcifications, suggestive of a retained appendicolith with abscess formation. this case illustrates the importa ...201020930600
endogenous endophthalmitis with brain abscesses caused by streptococcus constellatus. 201121482882
[a method for differential identification of group c and g streptococci with pcr].the paper describes a rapid method for pcr identification of groups c and g streptococci (streptococcus dysgalactiae subspecies equisimilis, streptococcus constellatus, and streptococcus anginosus) that cause human disease. species-specific regions of the cpn60 gene encoding heat shock protein groel (hsp60) were chosen as markers for pcr diagnosis; three pairs of primers were constructed for these regions, each of which was peculiar to the specific type. the method was tested on a large collecti ...201121506385
spiramycin resistance in human periodontitis microbiota.purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ...201121524712
fatal subdural empyema caused by streptococcus constellatus and actinomyces viscosus in a child-case report.group milleri streptococci that colonize the mouth and the upper airways are generally considered to be commensal. in combination with anaerobics, they are rarely responsible for brain abscesses in patients with certain predisposing factors. mortality in such cases is high and complications are frequent. we present a case of fatal subdural empyema caused by streptococcus constellatus and actinomyces viscosus in a previously healthy 7-year-old girl.201121524967
sudden onset proptosis secondary to cavernous sinus thrombosis from underlying mandibular dental infection.the present report concerns a patient who presented with a 4-day history of left-sided facial pain arising from a pre-existing dental infection and progressive shortness of breath. the patient had a previous diagnosis of rheumatoid arthritis and was being treated with methotrexate. the rapid development of a right eye proptosis necessitated urgent decompression with a lateral canthotomy and cantholysis. imaging revealed a left facial abscess, cavernous sinus thrombosis (cst), bilateral internal ...200921789100
[Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between April 2009 and March 2010].Bacteria isolated from surgical infections during the period from April 2009 to March 2010 were investigated in a multicenter study in Japan, and the following results were obtained. In this series, 671 strains including 16 strains of Candida spp. were isolated from 174 (79.1%) of 220 patients with surgical infections. Four hundred and eleven strains were isolated from primary infections, and 244 strains were isolated from surgical site infection. From primary infections, anaerobic Gram-negative ...201121861307
streptococcus constellatus-associated pyoderma in a dog.this report describes a case of chronic and deep pyodermitis in a four-year-old male dog with three-months skin problems history that had been treated unsuccessfully with fluoroquinolone therapy, prescribed by a private medical veterinary practice, without an early diagnosis.microbiological examination and antimicrobial susceptibility testing were performed in our laboratory (faculty of veterinay medicine) and a diagnosis of streptococcus constellatus-associated pyoderma in the dog was made. a n ...201122074849
extracellular deoxyribonuclease production by periodontal bacteria.palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective:  whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ...201122150619
[Multiple complications of acute unilateral rhinosinusitis--a case report].Intracranial or orbital complications after chronic and acute rhinosinusitis are rare, but could be dangerous. The complications of acute rhinosinusitis more often are observed in young patients. The combination of both intracranial and orbital complications together is found after acute inflammation.201121916225
extra-corporeal membrane oxygenation in a patient with fusobacterium sepsis: a case report and review of literature.an adolescent female was admitted to the pediatric intensive care unit in septic shock. she developed multisystem organ dysfunction including pancreatitis with myocardial dysfunction and hemodynamic instability unresponsive to medical management necessitating veno-arterial extracorporeal support. streptococcus constellata and fusobacterium necrophorum were isolated from blood cultures. this is the first report of extra-corporeal cardiac support in fusobacterium sepsis.201121881329
molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard dna-dna hybridization.knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. the purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 dna probes for different bacterial species by using the checkerboard dna-dna hybridization (cddh) technique.201222196182
[Pleural empyema caused by Streptococcus constellatus]. 201122093136
development and pyrosequencing analysis of an in-vitro oral biofilm model.dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. the principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. in-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. the aim of this study was to develop an oral biofilm model using the calgary biofilm device (cb ...201525880819
oral microbiota species in acute apical endodontic abscesses.acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions.201626983837
tobramycin-treated pseudomonas aeruginosa pa14 enhances streptococcus constellatus 7155 biofilm formation in a cystic fibrosis model system.cystic fibrosis (cf) is a human genetic disorder which results in a lung environment that is highly conducive to chronic microbial infection. over the past decade, deep-sequencing studies have demonstrated that the cf lung can harbor a highly diverse polymicrobial community. we expanded our existing in vitro model of pseudomonas aeruginosa biofilm formation on cf-derived airway cells to include this broader set of cf airway colonizers to investigate their contributions to cf lung disease, partic ...201526483523
rare purulent cardiac tamponade caused by streptococcus constellatus in a young immunocompetent patient: case report and review of the literature.background purulent pericardial tamponade is a very rare occurrence in the current era of widespread antibiotic use. it is even rarer when caused by streptococcus constellatus: a microorganism usually classified among the normal flora of the human body. it is occasionally diagnosed with certain predisposing factors. case report we present the third case of streptococcus constellatus cardiac tamponade reported in the current medical literature, occurring in a previously healthy young man who was ...201627847383
impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray.this study compares the changes to the subgingival microbiota of individuals with "refractory" periodontitis (rp) or treatable periodontitis (good responders [gr]) before and after periodontal therapy by using the human oral microbe identification microarray (homim) analysis.201222324467
multiple liver abscesses and bacteremia caused by streptococcus constellatus infection: a case report.the streptococcus milleri group (smg) comprises commensal mucosal bacteria. pyogenic liver abscesses due to hematogenous smg infection are rare but can be observed even in healthy patients. in such cases, physicians should consider the existence of primary lesions that allow penetration of the smg.201628096994
osseointegrated implants placed at supracrestal level may harbour higher counts of a. gerencseriae and s. constellatus - a randomized, controlled pilot study.this study aimed at evaluating the bacterial colonization in dental implants inserted in the crestal or supracrestal position and correlated it to radiographic bone measurements.201526499108
the predominant bacteria isolated from radicular cysts.to detect predominant bacteria associated with radicular cysts and discuss in light of the literature.201324011184
subgingival microbiota in adult down syndrome periodontitis.the subgingival microbiota in down syndrome and non-down syndrome adults receiving periodic dental care was examined for 40 bacterial species using checkerboard dna-dna hybridization and the results were related to clinical periodontal attachment loss.201222221039
antibiotic resistance in human chronic periodontitis microbiota.patients with chronic periodontitis (cp) may yield multiple species of putative periodontal bacterial pathogens that vary in their antibiotic drug susceptibility. this study determines the occurrence of in vitro antibiotic resistance among selected subgingival periodontal pathogens in patients with cp.201423688097
relapse of polymicrobial endocarditis in an intravenous drug user.a 26-year-old male intravenous drug user (idu) presented twice within 6 months with relapsed polymicrobial infective endocarditis (ie) due to eikenella corrodens and streptococcus constellatus after completing two courses of appropriate antimicrobial therapy. this report points to relapsing endocarditis as a clinical entity that warrants attention in idus when e. corrodens or s. constellatus are causative agents of ie.201121966051
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2010 and march 2011].bacteria isolated from surgical infections during the period from april 2010 to march 2011 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 631 strains including 25 strains of candida spp. were isolated from 170 (81.7%) of 208 patients with surgical infections. four hundred and twenty two strains were isolated from primary infections, and 184 strains were isolated from surgical site infection. from primary infections, anaerobic gram-nega ...201425549405
brain abscess potentially resulting from odontogenic focus: report of three cases and a literature review.odontogenic foci can rarely cause intracranial infection. hematogenous spread is considered to be the most important pathophysiological mechanism of intracranial infection of odontogenic origin. to investigate the oral origin of intracranial infections, oral surgeons should understand the underlying mechanisms by which oral bacteria spread to the central nervous system. however, there have been very few reports of intracranial infection resulting from odontogenic infection.201728286386
profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws.microbial etiology for anti-osteoclastic drug-related osteonecrosis of the jaw (aronj) was suggested. this study investigates any link between bacteria colonizing aronj sites and other oral cavity sites. microbiota samples of 10 aronj patients were collected from the exposed bone, adjacent teeth, contralateral teeth, and tongue. dna checkerboard hybridization was used for microbiota analysis with 43 genomic dna probes prepared from human oral bacterial (38) and candida (5) species, using socrans ...201627419922
microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation.this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp).201526521147
effect of smokeless tobacco products on human oral bacteria growth and viability.to evaluate the toxicity of smokeless tobacco products (stps) on oral bacteria, seven smokeless tobacco aqueous extracts (staes) from major brands of stps and three tobacco-specific n-nitrosamines (tsnas) were used in a growth and viability test against 38 oral bacterial species or subspecies. all seven staes showed concentration-dependent effects on the growth and viability of tested oral bacteria under anaerobic culture conditions, although there were strain-to-strain variations. in the presen ...201627756619
relationship between periodontitis-associated subgingival microbiota and clinical inflammation by 16s pyrosequencing.to analyse the relationship between the chronic periodontitis-associated subgingival microbiota and clinical inflammation.201526461079
anaerobic oral flora in the north american black bear (ursus americanus) in eastern north carolina.microbial flora can provide insight into the ecology and natural history of wildlife in addition to improving understanding of health risks. this study examines the anaerobic oral flora of hunter killed black bears (ursus americanus) in eastern north carolina. oral swabs from the buccal and lingual supragingival tooth surfaces of the first and second mandibular and maxillary molars of 22 black bears were inoculated onto brucella blood agar plates supplemented with hemin and vitamin k after trans ...201222503889
antibiotic resistance in human peri-implantitis microbiota.because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of in vitro antibiotic resistance among putative peri-implantitis bacterial pathogens.201423551701
[descending necrotizing mediastinitis extended to empyema].a 61-year-old man came to our hospital complained of neck swelling after extracting a tooth. cervical drainage was performed in the diagnosis of cervical abscess. two days later, left pleural effusion appeared and its bacteriologic culture showed streptococcus constellatus. computed tomography (ct) scan showed massive retained pus in the mediastinum. thoracic drainage alone wasn't effective and the left thoracotomy was immediately performed to open the mediastinal pleura and curette the thoracic ...201121387621
lemierre syndrome with blepharoptosis.a 51-year-old woman was hospitalized with a high fever, occipital pain, blepharoptosis, and trismus. enhanced ct showed thrombophlebitis of her left cavernous sinus, maxillary vein, and multiple pulmonary nodular lesions. (18)f-fdg pet/ct showed significant uptakes in the same lesions. streptococcus constellatus was detected in her blood. therefore, she was diagnosed as a lemierre syndrome variant. after administration of antibiotics, all symptoms, inflammatory reactions, and thrombi disappeared ...201020424365
multiple pyogenic liver abscesses caused by streptococcus constellatus in the amazon region. case report.there are few studies reporting pyogenic liver abscess (pla) caused by streptococcus constellatus in the medical literature. s. constellatus is a comensal microorganism that belongs to the streptococcus milleri's bacteria group and is not considered to be pathogenic for humans. we report the case of a 23-year-old man with a 15-days history of abdominal pain in the right flank followed by daily fever, chills, nausea, vomits, sialism and jaundice. physical examination revealed moderate jaundice (2 ...200919841508
neck mass in a rheumatoid arthritis patient taking etanercept.abstract: a 60-year-old caucasian woman with a history of rheumatoid arthritis and a remote history of a post-traumatic splenectomy developed a serious head and neck infection 11 weeks after beginning therapy with etanercept. the patient required incision and drainage of a neck abscess and 2 weeks of i.v. antibiotics to achieve complete recovery. her etanercept was also discontinued. the causative organism was streptococcus constellatus, which is a member of the group of encapsulated organisms k ...200019078454
case-control study of pneumonia patients with streptococcus anginosus group bacteria in their sputum.in recent years, streptococcus anginosus group (sag) bacteria are becoming increasingly recognized as important pneumonia-causing pathogens. although several small studies have been reported, the features of sag pneumonia remain unclear, because the identification of sag from sputum cultures is not routinely performed in most microbiology laboratories. the aim of this study was to elucidate the clinical characteristics of sag pneumonia.201627681235
streptococcus anginosus (milleri) group strains isolated in poland (1996-2012) and their antibiotic resistance patterns.streptococcus anginosus, streptococcus intermedius and streptococcus constellatus form a group of related streptococcal species, namely the streptococcus anginosus group (sag). the group, previously called "milleri" had been rarely described until 1980/1990 as source of infections. nowadays sag bacteria are often described as pathogens causing predominantly purulent infections. the number of infections is highly underestimated, as sag strains are often classified in the microbiology laboratory a ...201627281992
the streptococcus milleri group in chronic obstructive pulmonary disease.s. anginosus, constellatus and intermedius, also known as the streptococcus milleri group (smg) are three streptococcal species more frequently detected in cases of invasive disease, abscesses and empyema in particular. recent research suggests they play a role in exacerbations of cystic fibrosis (cf). owing to poor recovery on standard culture media and difficult differentiation from non-pathogenic streptococci, smg may be underdiagnosed in routine settings. we aimed to establish the incidence ...201627132809
aortoduodenal fistula and streptococcal myonecrosis.pyomyositis is a rare primary bacterial infection of the skeletal muscles. pyomyonecrosis is the most severe manifestation of this disease and is associated with a potentially devastating outcome. patients with peripheral vascular disease presenting with pyomyositis may be difficult to distinguish from those with critical ischemia or synthetic graft sepsis. this article reports on a patient with aortobifemoral bypass graft and severe vitamin b(12) deficiency who developed pyomyonecrosis and aort ...200818667464
streptococcus constellatus causing septic thrombophlebitis of the right ovarian vein with extension into the inferior vena cava.introduction. streptococcus constellatus collectively with streptococcus anginosus and streptococcus intermedius constitute the streptococcus anginosus (formerly streptococcus milleri) group. though they are commonly associated with abscesses, bacteremia with subsequent septic thrombophlebitis is extremely rare, and resulting mortality is infrequent. case presentation. we report a case of a previously healthy 60-year-old african american female who presented with streptococcus constellatus bacte ...201526171262
identification of the 'streptococcus anginosus group' by matrix-assisted laser desorption ionization--time-of-flight mass spectrometry.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) provides rapid, accurate and cost-effective identification of a range of bacteria and is rapidly changing the face of routine diagnostic microbiology. however, certain groups of bacteria, for example streptococci (in particular viridans or non-haemolytic streptococci), are less reliably identified by this method. we studied the performance of maldi-tof ms for identification of the 'streptococcus anginosus ...201424917618
molecular pathogenicity of streptococcus anginosus.streptococcus anginosus and the closely related species streptococcus constellatus and streptococcus intermedius, are primarily commensals of the mucosa. the true pathogenic potential of this group has been under-recognized for a long time because of difficulties in correct species identification as well as the commensal nature of these species. in recent years, streptococci of the s. anginosus group have been increasingly found as relevant microbial pathogens in abscesses and blood cultures and ...201424848553
an unusual case of streptococcus anginosus group pyomyositis diagnosed using direct 16s ribosomal dna sequencing.bacteria belonging to the streptococcus anginosus group (streptococcus intermedius, streptococcus constellatus and streptococcus anginosus) are capable of causing serious pyogenic infections, with a tendency for abscess formation. the present article reports a case of s anginosus group pyomyositis in a 47-year-old man. the pathogen was recovered from one of two blood cultures obtained from the patient, but speciation was initially not performed because the organism was considered to be a contami ...201424634686
a streptolysin s homologue is essential for β-haemolytic streptococcus constellatus subsp. constellatus cytotoxicity.streptococcus constellatus is a member of the anginosus group streptococci (ags) and primarily inhabits the human oral cavity. s. constellatus is composed of three subspecies: s. constellatus subsp. constellatus (scc), s. constellatus subsp. pharyngis and the newly described subspecies s. constellatus subsp. viborgensis. although previous studies have established that scc contains β-haemolytic strains, the factor(s) responsible for β-haemolysis in β-haemolytic scc (β-scc) has yet to be clarified ...201424600025
invasive streptococcus anginosus group infection-does the species predict the outcome?to determine whether there is an association between the species of streptococcus anginosus group (sag) bacteria and the clinical outcome.201424215937
identification, clinical aspects, susceptibility pattern, and molecular epidemiology of beta-haemolytic group g streptococcus anginosus group isolates from central taiwan.no literature is available on the prevalence and clinical aspects of beta-haemolytic group g streptococcus anginosus group in central taiwan. in this study, we used 16s rrna gene sequencing and 16s-23s rdna intergenic spacer sequencing (where necessary) as the gold standard for molecular identification. twenty-seven s. anginosus group isolates were identified from 273 beta-haemolytic ggs isolates collected from patients in central taiwan between february 2007 and august 2011. of the 27 isolates, ...201323632249
identification of β-haemolysin-encoding genes in streptococcus anginosus.streptococcus anginosus is an emerging pathogen, but little is known about its virulence factors. to detect the genes responsible for β-haemolysis we performed genomic mutagenesis of the β-haemolytic s. anginosus type strain atcc 12395 using the vector pghost9:iss1. integration site analysis of 15 non-haemolytic mutants identified a gene cluster with high homology to the genes of the streptolysin s (sls) encoding sag gene cluster of s. pyogenes. the gene cluster harbours 10 open reading frames d ...201323594064
streptococcus constellatus and prevotella bivia penile abscess.streptococcus constellatus (s. constellatus) is known to cause abscesses in the oral, genitourinary, and gastrointestinal tracts, frequently in association with anaerobic bacteria. we report a rare case of s. constellatus and prevotella bivia (p. bivia) causing a penile abscess, which was successfully treated with surgical drainage and antibiotic treatment.200717982605
embolic myocardial infarction and left ventricular rupture due to mitral valve endocarditis.a left ventricular rupture due to embolic myocardial infarction is extremely rare. a 72-year-old woman developed an acute embolic myocardial infarction and mitral regurgitation due to infective endocarditis. two days after the infarction, a left ventricular free wall rupture occurred after transesophageal echo examination. she received an epicardial patch and mitral valve replacement. perioperatively, an intra-aortic balloon pump and long-term antibiotics were used. the postoperative course was ...200717592432
real-time monitoring of the adherence of streptococcus anginosus group bacteria to extracellular matrix decorin and biglycan proteoglycans in biofilm formation.members of the streptococcus anginosus group (sags) are significant pathogens. however, their pathogenic mechanisms are incompletely understood. this study investigates the adherence of sags to the matrix proteoglycans decorin and biglycan of soft gingival and alveolar bone. recombinant chondroitin 4-sulphate(c4s)-conjugated decorin and biglycan were synthesised using mammalian expression systems. c4s-conjugated decorin/biglycan and dermatan sulphate (ds) decorin/biglycan were isolated from ovin ...201222835945
brain abscess following dental procedures. case report.there have been a number of reports of brain abscess suggesting an odontogenic etiology after dental treatment procedures or oral infections, but the diagnosis was mainly by exclusion rather than based on evidence. we present a case of a brain abscess due to strepto-coccus constellatus in a 27-year-old-immunocompetent woman who underwent extraction of the primary molar and eradication of a radicular cyst some weeks before.200717529917
pyopneumothorax secondary to streptococcus milleri infection.we report a case of a 46-year-old malay woman with underlying hypothyroidism post thyroidectomy who presented with worsening breathlessness, orthopnoea, productive cough and left-sided pleuritic chest pain of 3 days duration. chest x-ray on admission showed left-sided massive hydropneumothorax. pleural tapping revealed empyema. a chest tube was inserted immediately. the culture of pleural fluid grew streptococcus constellatus the patient was treated with antibiotics for a total of 6 weeks and un ...201627881590
thalamic abscess caused by a rare pathogen: streptococcus constellatus.streptococcus constellatus is a microorganism that lives commensally in the oropharyngeal region, urogenital region, and intestinal tract. however, it can cause infection in patients with certain predisposing factors. rarely, this microorganism can cause a brain abscess. thalamic localization of brain abscesses is much rarer than abscesses in other locations of the brain. brain abscess caused by streptococcus constellatus are very rarely been reported in the literature. we present a rare case of ...201627800109
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