| protective effects of polyclonal sera and of monoclonal antibodies active to salmonella minnesota re595 lipopolysaccharide during experimental endotoxemia. | mice were passively immunized with sera from blood donors active for rough lipopolysaccharides (lps), the j5 (rc chemotype) mutant of escherichia coli o111:b4, and the re595 (re chemotype) mutant of salmonella minnesota. all protected the mice against lethal challenge with smooth e. coli wf96 lps, e. coli and salmonella rough mutant lps, or free lipid a. epitopes recognized by monoclonal antibodies (mabs) reacting with the lps of s. minnesota re595 or lipid a were localized in the 2-keto-3-deoxy ... | 1990 | 1700024 |
| binding of enteric bacteria to hog gastric mucin. | the binding features of enteric bacteria were studied using a model mucin of hog gastric origin. the time requirement of binding is short, it is temperature-independent, but dose-dependent. the binding effectiveness of escherichia coli, shigella sonnei and shigella flexneri, as well as salmonella minnesota had a narrow range: 1.5-9 germs pro pg of mucin. the bacterial ligand of the binding is certainly not a polysaccharide as proved by the uniform binding of the r-mutant series of s. sonnei and ... | 1990 | 1702253 |
| studies on the interaction of c1q, a subcomponent of the first component of complement, with porins from salmonella minnesota incorporated into artificial membranes. | purified outer membrane proteins (omp) of salmonella minnesota, re-form, were incorporated into liposomes. these induced in macrophages a chemiluminescence signal identical to that of the intact re-form. this signal was abolished by preincubation of porin-containing liposomes with purified c1q. incorporation of isolated omp into black lipid membranes (blm) resulted in channel-formation which could not be inhibited by isolated c1q. additionally, incubation of omp-containing liposomes with blm res ... | 1990 | 1702394 |
| bacterial lipopolysaccharide stimulates protein tyrosine phosphorylation in macrophages. | lipopolysaccharide (lps), a membrane component of gram-negative bacteria, stimulates immune responses by activating macrophages, b lymphocytes, and other cells of the immune system. the mechanisms by which lps activates these cells are poorly characterized. since protein tyrosine phosphorylation appears to be a major intracellular signaling event that mediates cellular responses, we examined whether lps alters tyrosine phosphorylation in macrophages. we found that escherichia coli k235 lps incre ... | 1991 | 1709735 |
| effect of salmonella bacteria on the interaction of human nk cells with endothelial cells. | leukocyte-endothelial cell adhesion and its regulation are essential and complex initial aspects of lymphocyte migration. various factors (il-1, tnf-alpha, ifn-gamma etc.) have been shown to increase the endothelial adhesiveness for human lymphocytes, including natural killer cells (nk cells). in this work we have demonstrated that pretreatment of either the target endothelial cell monolayers or the binding lgl-cells with mr595 salmonella minnesota bacteria results in a substantial increase in t ... | 1991 | 1710821 |
| lysis of neisseria gonorrhoeae initiated by binding of normal human igm to a hexosamine-containing lipooligosaccharide epitope(s) is augmented by strain-specific, properdin-binding-dependent alternative complement pathway activation. | we studied the specificity of naturally acquired igm bactericidal for strains of neisseria gonorrhoeae that varied in sensitivity to the lytic action of normal human serum (nhs) and the relative ability of these strains to deplete the classical (cp) and alternative (acp) c pathways. lysis of both highly sensitive and relatively insensitive strains was inhibited by the same gonococcal lipooligosaccharides (los), as well as by salmonella minnesota re los and three hexosamine-containing glycose pol ... | 1991 | 1711080 |
| binding sites for endotoxins (lipopolysaccharides) on human monocytes. | the nature of the binding sites for lps on human monocytes was investigated using [3h] labeled intact lps from neisseria meningitidis and from salmonella minnesota r7, and the [3h] labeled purified inner core region (ps-ome) of s.m. r7 lps. in the presence of serum, intact lps from enterobacterial and nonenterobacterial strains bound to monocytes in a dose-dependent, saturable, and displaceable fashion. n.m. lps and lps from the enterobacterial strain of escherichia coli 0111-b4 bound to the sam ... | 1991 | 1716284 |
| lipopolysaccharide-induced stimulation of cd11b/cd18 expression on neutrophils. evidence of specific receptor-based response and inhibition by lipid a-based antagonists. | gram-negative bacterial septicemia is a common clinical syndrome resulting, in part, from the activation of phagocytic leukocytes by lps. by using flow cytometry, we have characterized lps-induced expression of the beta 2 integrin cd11b/cd18. after exposure to salmonella minnesota r595 lps, expression of neutrophil cd11b/cd18 is rapidly upregulated, beginning within 5 min and achieving a peak fluorescence (typically two- to threefold over base line) by 30 min. the increase in cd11b/cd18 expressi ... | 1991 | 1717586 |
| production of monoclonal antibodies specific for ganglioside gd3. | four kinds of anti-gd3 monoclonal antibodies, dsg-1, -2, -3, and -4, of the igm class were obtained by the immunization of balb/c mice with enzootic bovine leukosis tumor tissue-derived ganglioside gd3 inserted into liposomes with salmonella minnesota r595 lipopolysaccharides. the specificities of the monoclonal antibodies obtained were defined by complement-dependent liposome immune lysis assay and by enzyme immunostaining on thin-layer chromatography. the reactivities of the monoclonal antibod ... | 1991 | 1724444 |
| detection of lipopolysaccharide-binding proteins on membranes of murine lymphocyte and macrophage-like cell lines. | lipopolysaccharide-(lps) binding proteins present on murine-lymphocyte and macrophage-like cell lines were identified by a ligand-blotting method and subsequent immunological detection of bound lps. membrane proteins of the murine-pre-b-cell line 70z/3 were separated by sds-page, transferred electrophoretically onto nitrocellulose, and the blot was incubated with lps of the salmonella minnesota re-mutant r595 (mre-lps). lps bound to proteins on nitrocellulose was immunologically detected by anti ... | 1991 | 1725955 |
| the salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagc and ail. | a fragment of the salmonella typhimurium virulence plasmid containing the rck locus, when cloned in the recombinant cosmid pade016, was shown previously to confer high-level complement resistance on both rough and smooth escherichia coli, salmonella minnesota, and s. typhimurium and was associated with the production of an outer membrane protein. we determined the nucleotide sequence of the fragment containing the rck locus. mutations in the two major open reading frames confirmed that the compl ... | 1992 | 1729227 |
| in vitro formation of complement activation products by lipopolysaccharide chemotypes of salmonella minnesota. | we have applied immunoassays for complement activation products c4d, fragment bb and the protein s-c5b-9 neoantigen (s-mac) to assess activation of classical, alternative and terminal pathways, respectively, by lipopolysaccharides (lps) from the smooth strain (ss) of salmonella minnesota and the shallow rough (core) mutants r60, r345, r5 and r7. incubations of sera (n = 6) with lps generated small and insignificant quantities of bb and s-mac in the case of rb, rc and rd chemotypes and slightly g ... | 1991 | 1752696 |
| aminoglycosides modify the in vitro metachromatic reaction and murine generalized shwartzman phenomenon induced by salmonella minnesota r595 lipopolysaccharide. | endotoxin-neutralizing activity may be an important property for antibiotics to be used in severe sepsis. several antibiotics, belonging to different classes, were evaluated as to their endotoxin-neutralizing ability, using the inhibition of an in vitro metachromatic assay for lipopolysaccharides and a murine generalized shwartzman reaction model. gentamicin, amikacin, and sisomicin have been found to share significant in vitro antiendotoxin activity at an antibiotic/endotoxin ratio as low as 1. ... | 1991 | 1759842 |
| binding profiles of anticardiolipin antibodies in sera from patients with sle and infectious diseases. | inhibition experiments were performed to study the specificity of igg-class antibody, binding to cardiolipin immobilized onto a polystyrene surface, in sera from patients with systemic lupus erythematosus (sle) or infection. six different phospholipids (three anionic: cardiolipin, phosphatidylserine and phosphatidic acid, and three neutral: phosphatidylcholine, phosphatidylethanolamine and platelet activating factor), lipopolysaccharide from salmonella minnesota (relps), strain re595 and lipotei ... | 1991 | 1797029 |
| structural studies of the core region of the lipopolysaccharide from salmonella minnesota strain r7 (rough mutant chemotype rd1). | | 1991 | 1804536 |
| antibodies to core lipid fractions of endotoxin in children with rheumatic disease. | the titer of igg and igm antibodies to two fragments of endotoxin derived from salmonella minnesota r 595, lipid a width a 2-keto-3-deoxyoctanate oliosaccharide (kdo lipid a), and monophosphoryl lipid a (mono-p lipid), were measured in normal children, children with cystic fibrosis, and children with sle and all forms of chronic juvenile arthritis. elevated titers of igm anti mono-p lipid a were found in all of the rheumatic diseases, but not in cystic fibrosis. the elevated igm titers were not ... | 1991 | 1845405 |
| a new monoclonal antibody directed to sialyl alpha 2-3lactoneotetraosylceramide and its application for detection of human gastrointestinal neoplasms. | a new monoclonal antibody (ns24) directed to the n-acetylneuraminyl alpha 2-3gal beta 1-4glcnac residue in type ii sugar chain of n-acetylneuraminyllactoneotetraosylceramide [sialylparagloboside, iv3(neuac)nlc4cer] was prepared by hybridoma technique. liposomes composed of dipalmitoylphosphatidylcholine, cholesterol, iv3(neuac)nlc4cer, and lipopolysaccharides from salmonella minnesota r595 were used for immunization with iv3(neuac)nlc4cer isolated from human erythrocytes. this method allowed the ... | 1991 | 1864848 |
| endotoxin-neutralizing properties of the 25 kd n-terminal fragment and a newly isolated 30 kd c-terminal fragment of the 55-60 kd bactericidal/permeability-increasing protein of human neutrophils. | the bactericidal/permeability-increasing protein (bpi) of polymorphonuclear leukocytes (pmn) is a potent cytotoxin, specific for gram-negative bacteria, that also inhibits endotoxin activity by neutralizing isolated bacterial lipopolysaccharides (lps). we have previously shown that an isolated 25 kd n-terminal fragment of human bpi carries all the antibacterial activities of the parent 55-60 kd molecule. in this study we have compared the lps-neutralizing activities of human holo-bpi, the n-term ... | 1991 | 1875165 |
| analysis of unmodified endotoxin preparations by 252cf plasma desorption mass spectrometry. determination of molecular masses of the constituent native lipopolysaccharides. | nine unmodified endotoxin preparations constituted of re-, rd-, and rc-type lipopolysaccharides (2 to 5 glycoses), representing four species of enterobacteria were analyzed by 252cf plasma desorption mass spectrometry. the constituent lipopolysaccharides were characterized by the ion pair: (m-h)- and its corresponding lipid fragment ion. the lipid fragment ion is produced by cleavage of the glycosidic bond of the 3-deoxy-d-manno-oct-2-ulosonic acid unit that substitutes o-6' of the glucosamin be ... | 1991 | 1917976 |
| lipopolysaccharide responsiveness is an important factor in the generation of optimal antigen-specific t cell responses during infection with gram-negative bacteria. | we previously have found that the endotoxin (lps) of gram-negative bacteria is a major determinant of macrophage ia induction during infection with these organisms. specifically, i.p. injection of gram-negative bacteria elicits a striking macrophage ia response in lps-responder mice but virtually no response in lps-low-responder mice. as an extension of these findings, in this report we have tested the hypothesis that the inability of lps-low responder mice to mount an ia response during gram-ne ... | 1991 | 1918965 |
| neutralization of shwartzman-inducing activity by antibodies recognizing the re core or lipid a structures of lipopolysaccharide from salmonella minnesota r595 and pseudomonas vesicularis jcm1477. | antibodies recognizing the re core or lipid a structures of lipopolysaccharide (lps) derived from salmonella minnesota r595 and pseudomonas vesicularis jcm1477 were tested for the ability to neutralize the preparatory activity of endotoxin using the local shwartzman reaction. shwartzman-inducing activity of r595 lps (re-form) was strongly suppressed when the lps was incubated with the rabbit anti-r595 antiserum or the purified igg antibody which recognizes core region of the lps. the antiserum a ... | 1991 | 1921759 |
| endotoxin and lipid peroxidation in vivo in selenium- and vitamin e-deficient and -adequate rats. | the effect of salmonella typhimurium endotoxin injected intraperitoneally (0.5 mg/kg body weight) on lipid peroxidation in vivo was assessed. peroxidation was monitored by measuring ethane production, an autoxidation product of (n-3) unsaturated fatty acids. weanling rats were fed a selenium- and vitamin e-deficient basal diet or one supplemented with 0.2 mg se/kg and/or 200 mg vitamin e/kg. after 11 to 13 wk of feeding, ethane production was tripled in lps-treated se- and vitamin e-deficient ra ... | 1991 | 1995793 |
| bacterial lipopolysaccharide induces long-lasting iga deficiency concurrently with features of polyclonal b cell activation in normal and in lupus-prone mice. | polyclonal b cell activation (pba) and autoimmune disease can be induced in immunologically normal mice, or enhanced in lupus-prone mice, by bacterial lipopolysaccharide (lps). because immune defects are common in autoimmune diseases and iga deficiency is prevalent in patients with systemic lupus erythematosus, we investigated: (i) whether lps might induce iga deficiency in normal mice; (ii) whether iga deficiency might be a feature in lupus-prone mice; (iii) whether, if present in lupus-prone m ... | 1991 | 2015704 |
| inhibition by lipid a-specific monoclonal antibodies of priming of human polymorphonuclear leucocytes by endotoxin. | exposure to lipopolysaccharide (lps) primes polymorphonuclear leucocytes (pmnl) for enhanced release of oxygen metabolites after subsequent stimulation. the metabolic response of human pmnl primed with lps and stimulated with formyl-methionyl-leucyl-phenylalanine (fmlp) was measured by chemiluminescence (cl) as a parameter for endotoxic activity. polymyxin b (pmb) and monoclonal antibodies (mabs) with specificity for lipid a were tested for inhibition of the priming effect of re lps of salmonell ... | 1991 | 2020018 |
| mapping of the binding specificity for five monoclonal antibodies recognizing 3-deoxy-d-manno-octulosonic acid in bacterial lipopolysaccharides. | mouse mab were produced against the deep rough strains salmonella minnesota r 595, salmonella typhimurium sl 1102, and escherichia coli d21f2 and screened by enzyme immunoassay against lps of several chemotypes. five antibodies were selected for their ability to bind to chemotype deep rough (re) lps which has two 3-deoxy-d-manno-octulosonic acid (kdo) residues in its nonreducing end. structurally verified oligosaccharides isolated from rough lps and synthetic analogues of kdo were used in an enz ... | 1991 | 2033255 |
| monophosphoryl lipid a induces tolerance to the lethal hemodynamic effects of endotoxemia. | monophosphoryl lipid a (mpl) is a nontoxic derivative of lipid a. in this study, the induction of tolerance by mpl to the hemodynamic effects of lethal endotoxemia was tested. ten sprague-dawley rats were received either salmonella minnesota mpl 0.5 mg/100 g intravenously (i.v.) or equivalent volume of diluent (control) i.v. on day zero. on day 3 s. minnesota endotoxin (lps) 5.0 mg/100 g was administered i.v. cardiac output (co), arterial lactate (l), and central venous oxygen saturation (svo2) ... | 1991 | 2049817 |
| dissociation between limulus neutralisation and in vivo protection in monoclonal antibodies directed against endotoxin core structures. | studies with rough mutants of certain gram-negative bacteria have indicated that monoclonal antibodies (mabs) to endotoxin core can protect animals and man from endotoxic shock. we assessed the ability of such antibodies to neutralise endotoxin in the limulus amoebocyte lysate (lal) assay, and compared this to their protective effect in a murine model of endotoxic shock. we evaluated 11 mabs raised against salmonella minnesota r595. endotoxin neutralisation in the lal assay, expressed as 50% inh ... | 1990 | 2077344 |
| phase diagram of lipid a from salmonella minnesota and escherichia coli rough mutant lipopolysaccharide. | we have reported here on the structural polymorphism of lipid a, the "endotoxic principle" of bacterial lipopolysaccharide. for lipid a of rough mutant lipopolysaccharide from salmonella minnesota and escherichia coli, the three-dimensional supramolecular structures were determined with x-ray diffraction utilizing synchrotron radiation. the investigations were performed in the water concentration range 10 to 95% by weight, at [lipid a]:[mg2+] molar ratios from 1:0 to 0.1:1, and in the temperatur ... | 1990 | 2100144 |
| further characterization of monoclonal antibodies to lipopolysaccharide of salmonella minnesota strain r595. | we have shown here that despite the use of monoclonal antibodies with well-defined epitope-specificities, and despite testing them in the most simple animal model available (i.e., mixing of homologous lps with mab prior to injection), we are not yet able to explain why some of the antibodies were effective and others not. for some of the clones (e.g., clone 20), an even better definition of binding sites is currently taking place in an attempt to obtain this understanding. we also do not yet und ... | 1990 | 2109505 |
| [stimulation of nonspecific antibacterial resistance of mice to opportunistic gram-negative microorganisms with triterpene glycosides from holothuroidea]. | a total fraction of triterpenic glycosides from cucumaria japonica named cucumarioside was used as a stimulator of nonspecific resistance to bacterial infections in mice. after intraperitoneal administration to mice subsequently infected with various strains of e. coli and proteus mirabilis, cucumarioside provided survival of 40 to 90 per cent of the infected animals against 100 per cent in the control group. the protective effect directly depended on the dose of cucumarioside. it was optimal to ... | 1990 | 2110443 |
| priming of polymorphonuclear granulocytes by lipopolysaccharides and its complexes with lipopolysaccharide binding protein and high density lipoprotein. | human peripheral blood neutrophils are primed, or enabled to respond to formyl peptide, by prior exposure to bacterial lipopolysaccharide (lps). the activity of lps and the size of its aggregates are altered by plasma constituents such as high density lipoprotein (hdl) and the recently discovered acute phase reactant lipopolysaccharide binding protein (lbp) tobias et al.: j. exp. med. 164,777, 1986]. the ability of lps, lps-lbp, and lps-hdl complexes to activate a number of cellular responses ha ... | 1990 | 2154525 |
| binding of rough lipopolysaccharides (lps) to human leukocytes. inhibition by anti-lps monoclonal antibody. | the binding of rough lps (relps from salmonella minnesota r595) to human peripheral blood polymorphonuclear leukocytes (pmn), monocytes, and lymphocytes was examined by using fluorescein-labeled lps and flow cytometry. at 4 degrees c, fitc-relps bound rapidly in a concentration- and time-dependent way to pmn, monocytes, and lymphocytes. because mononuclear cells showed both binding and nonbinding cell populations, fitc-relps was used in conjunction with specific phycoerythrin-labeled mab to iden ... | 1990 | 2162891 |
| monophosphoryl lipid a inhibits neutrophil priming by lipopolysaccharide. | it is known that lipopolysaccharides (endotoxin) prime neutrophils for oxygen radical production. monophosphoryl lipid a is a nontoxic derivative of lipid a that protects against lethal endotoxemia. we examined the effects of salmonella minnesota monophosphoryl lipid a on s. minnesota lipopolysaccharide-induced priming of neutrophil superoxide anion generation. human neutrophils were preincubated with and without either lipopolysaccharide or monophosphoryl lipid a before stimulation with 10(-5) ... | 1990 | 2168464 |
| transfer of preformed terminal c5b-9 complement complexes into the outer membrane of viable gram-negative bacteria: effect on viability and integrity. | an efficient fusion system between gram-negative bacteria and liposomes incorporating detergent-extracted c5b-9 complexes has been developed that allows delivery of preformed terminal complexes to the cell envelope (tomlinson et al., 1989b). fusion of salmonella minnesota re595 and escherichia coli 17 with c5b-9-incorporated liposomes resulted in the transfer of 1900 c5b-9 complexes to each target bacterial cell. no loss in viability of bacteria was observed following fusion, even though the dep ... | 1990 | 2184889 |
| relationship between mg2(+)-induced hexagonal assembly of r-form lipopolysaccharides and chemical structure of their r-cores. | the relationship between formation of the mg2(+)-induced hexagonal lattice structure by r-form lipopolysaccharides (lps) and chemical structure of their r-cores was investigated using different kinds of r-form lps from a series of mutants of salmonella minnesota or s. typhimurium. the optimal experimental condition for formation of the hexagonal lattice structure was to suspend lps preparations, from which cationic material was removed by electrodialysis, in 50 mm tris (hydroxymethyl) aminometha ... | 1990 | 2191197 |
| investigation into the fluidity of lipopolysaccharide and free lipid a membrane systems by fourier-transform infrared spectroscopy and differential scanning calorimetry. | the phase behaviour, particularly the fluidity within each phase state and the transitions between them, of lipopolysaccharides and of their lipid moiety, free lipid a, of various species of gram-negative bacteria, especially of salmonella minnesota and escherichia coli, has been investigated by applying mainly fourier-transform infrared spectroscopy and differential scanning calorimetry. for enterobacterial strains, the transition temperatures of the gel----liquid crystalline (beta----alpha) ph ... | 1990 | 2199198 |
| [proteus peritonitis-bacteremia in mice--a model for studying postvaccinal proteus immunity]. | the dynamics of the formation of postvaccinal immunity after immunization with preparations obtained with the use of hydroxylamine (ha) preparations from proteus strains of different o serogroups, salmonella minnesota re-mutant and the common antimicrobial antigen isolated from escherichia coli 14 has been studied on mice with proteus peritonitis-bacteremia used as a model. the study has revealed that intraperitoneal immunization with proteus ha preparations stimulates the phagocytic activity of ... | 1990 | 2251896 |
| isolation and characterisation of 3-deoxy-d-manno-2-octulopyranosonate 7-(2-aminoethyl phosphate) from the inner core region of escherichia coli k-12 and salmonella minnesota lipopolysaccharides. | the title compound (pe-kdo) was isolated after hydrolysis of the lipopolysaccharides of escherichia coli k-12 strain w3100 and salmonella minnesota strains r4 and r7, and the location of the 2-aminoethyl phosphate group at position 7 was established by 13c-n.m.r. spectroscopy. derivatives of pe-kdo were acetylated, silylated, and methylated in order to evaluate their usefulness for analysis by g.l.c.-m.s. | 1990 | 2279250 |
| electron microscopic observation of crystals of escherichia coli k-12 lipopolysaccharide. | previously we showed that salmonella minnesota re and ra lipopolysaccharides (lpss) and escherichia coli k-12 lps formed three-dimensional crystals, either hexagonal plates or solid columns, when they were precipitated by the addition of 2 volumes of 95% ethanol containing 375 mm mgcl2 and incubated in 70% ethanol containing 250 mm mgcl2, and stored at 4 c for 10 days. later, escherichia coli k-12 lps thus treated was found to form discoid crystals as well as hexagonal plate crystals and solid c ... | 1990 | 2280724 |
| characterization of murine monoclonal and murine, rabbit, and human polyclonal antibodies against chlamydial lipopolysaccharide. | murine monoclonal and rabbit, murine, and human polyclonal antibodies against chlamydial lipopolysaccharide (lps) were characterized by the passive hemolysis and passive hemolysis inhibition assays and by absorption experiments with lpss of chlamydia psittaci, chlamydia trachomatis, and a recombinant strain of salmonella minnesota re (r595-207) expressing the chlamydia-specific lps epitope, as well as natural and synthetic partial structures of chlamydial lps. eleven monoclonal antibodies of the ... | 1990 | 2294050 |
| decreased tumor necrosis factor production during the initial stages of infection correlates with survival during murine gram-negative sepsis. | secretion of tumor necrosis factor (tnf)/cachectin occurs during gram-negative bacterial sepsis in response to macrophage stimulation by lipopolysaccharide (endotoxin) and may play an early pivotal role in the subsequent host response. we sought to determine whether administration of: (1) murine monoclonal antibody directed against endotoxin, (2) steroids, or (3) antimicrobial agents would abrogate tnf production and whether the protective capacity would correlate with tnf levels in an experimen ... | 1990 | 2294880 |
| a comparison of specificity and biological activity of polyclonal and monoclonal antibodies raised against salmonella minnesota r595 lipopolysaccharide. | murine monoclonal antibodies (mabs) and immune rabbit serum were raised against the rough mutant of salmonella minnesota strain r595. these antibodies were tested for their ability to inhibit lps-induced b-cell mitogenicity and neutralise lps toxicity in chick embryos. immune rabbit serum inhibited both mitogenicity and lps lethality. none of the mabs or a cocktail of antibodies were able to neutralise lps lethality in chick embryos. however, they were able to inhibit mitogenicity by varying deg ... | 1990 | 2304067 |
| comparisons of pasteurella multocida lipopolysaccharides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine relationship between group b and e hemorrhagic septicemia strains and serologically related group a strains. | lipopolysaccharides (lpss) purified from 16 reference somatic serotypes of pasteurella multocida were examined and compared by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis. resolution of lps patterns in a gel was optimum when sample wells were cast separately from the stacking gel and the running gel consisted of 15% t (total monomer) polyacrylamide and 4 m deionized urea. band patterns of p. multocida lpss in a gel differed from control salmonella minnesota wild-type ... | 1990 | 2332462 |
| structural characterization of monophosphoryl lipid a homologs obtained from salmonella minnesota re595 lipopolysaccharide. | sixteen monophosphoryl lipid a (mla) homologs obtained from the lipopolysaccharides of salmonella minnesota re595 were separated by preparative thin layer chromatography into eight fractions. the components of these fractions were analyzed directly (or as structural analogs) and characterized by mass spectrometry. molecular weights were determined by negative and positive ion fast atom bombardment mass spectrometry and component structures were assigned following a study of fragmentation and met ... | 1990 | 2335519 |
| endotoxic lipid a interaction with human platelets. structure-function analysis of lipid a homologs obtained from salmonella minnesota re595 lipopolysaccharide. | we previously reported that human blood platelets are directly stimulated by endotoxic lipid a via the protein kinase c pathway (grabarek, j., timmons, s., and hawiger, j. (1988) j. clin. invest. 82, 964-971). to study the relationship between the molecular structure of lipid a and its ability to activate human platelets, we used lipid a homologs derived from salmonella minnesota re595 lipopolysaccharide. preparations of lipid a are heterogeneous in regard to the degree of substitution of fatty ... | 1990 | 2335520 |
| identification of lipopolysaccharide-binding proteins in 70z/3 cells by photoaffinity cross-linking. | a radioiodinated, photoactivatable derivative of salmonella minnesota re595 lipopolysaccharide (lps) was used to label lps-binding proteins in 70z/3 cells. the labeled proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by autoradiography. 125i-labeled-2-(p-azidosalycylamido)1,3'-dithiopropionamide s. minnesota re595 lps (125i-asd-re595) labeled a limited number of proteins. the most prominent of these had a apparent molecular mass of 18 kda. less p ... | 1990 | 2345180 |
| fluorescence-activated cell sorter analysis of binding by lipopolysaccharide-specific monoclonal antibodies to gram-negative bacteria. | sixteen murine monoclonal antibodies (mabs) reactive with the o-side chain, core oligosaccharide, or lipid a of escherichia coli o111:b4 and salmonella minnesota lipopolysaccharide (lps) were evaluated for binding activity against wild-type and rough mutant strains using a fluorescence-activated cell sorter (facs) and fluorescein-conjugated antiimmunoglobulin probe. o-side-chain-reactive mabs produced immunofluorescence against homologous, smooth strains up to 500-fold higher than controls. many ... | 1990 | 2355191 |
| purification and characterization of lipopolysaccharide-binding protein from hemolymph of american cockroach periplaneta americana. | a protein having affinity to lipopolysaccharide of escherichia coli k12 was purified to homogeneity from the hemolymph of periplaneta americana. this protein, with an average molecular mass of 450 kda. was a homooligomer of a 28-kda subunit protein. comparative studies using lipopolysaccharide molecules of e. coli and salmonella minnesota suggested that this protein recognizes and binds to a specific carbohydrate structure of e. coli lipopolysaccharide. ca2+ was required for this protein to bind ... | 1990 | 2364946 |
| protection of mice against the lethal toxicity of a lipopolysaccharide (lps) by immunization with anti-idiotype antibody to a monoclonal antibody to lipid a from eikenella corrodens lps. | we produced anti-idiotype antibodies to antibody to lipid a from eikenella corrodens. the ala-1 monoclonal antibody (immunoglobulin m [igm] isotype), which had already been produced in our laboratory (t. kato, i. takazoe, and k. okuda, infect. immun. 57:656-659, 1989), had reacted strongly with lipid a from e. corrodens, escherichia coli, and salmonella minnesota. four anti-idiotype monoclonal antibodies to ala-1 (ab1), designated a2la-1 (igg1 isotype), a2la-2 (igg2a isotype), a2la-3 (igg2a isot ... | 1990 | 2404870 |
| characterization of anti-core glycolipid monoclonal antibodies with chemically defined lipopolysaccharides. | five anti-core glycolipid monoclonal antibodies (mab) (four against escherichia coli j5 lipopolysaccharide [lps] and one against the re core glycolipid of salmonella typhimurium) were characterized using lps from several rough and smooth strains and derivatives of e. coli j5 lps, obtained by n acetylation and hydrolysis. the mab against e. coli j5 were not only weakly cross-reactive with clinical isolates, whereas the anti-re mab was highly cross-reactive. the mab differed in their reaction patt ... | 1990 | 2404871 |
| crystallization of r-form lipopolysaccharides from salmonella minnesota and escherichia coli. | salmonella minnesota re and ra lipopolysaccharides (lpss) and escherichia coli k-12 lps formed three-dimensional crystals, either hexagonal plates (preferential growth along the a axis) or solid columns (preferential growth along the c axis), when they were precipitated by the addition of 2 volumes of 95% ethanol containing 375 mm mgcl2 and incubated in 70% ethanol containing 250 mm mgcl2 at 4 degrees c for 10 days. analyses of crystals suggested that they consist of hexagonal lattices with the ... | 1990 | 2407725 |
| comparison of the exoproducts of gram-negative bacteria by sds-page. | the protein exoproducts released during exponential growth of gram-negative bacteria were analysed and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the following bacterial strains were tested: escherichia coli, klebsiella pneumoniae, citrobacter freundii, enterobacter cloacae, serratia liquefaciens, serratia rubidaea, proteus mirabilis, proteus vulgaris, salmonella minnesota, pseudomonas aeruginosa and pseudomonas fluorescens. it is demonstrated by sds-page t ... | 1985 | 2408403 |
| monoclonal antibodies raised against neuac alpha 2-6neolactotetraosylceramide detect carcinoma-associated gangliosides. | monoclonal antibodies wee obtained by the immunization of mice with 6'lm1 (iv6neuac-nlcose4cer) adsorbed to salmonella minnesota. the monoclonal antibodies showed a specificity for gangliosides with a terminal neuac alpha 2-6gal substitution, which was demonstrated in solid-phase binding assay and in liposome inhibition assay. gangliosides with a neuac alpha 2-6gal substitution were minor components of different normal tissues. however, these gangliosides were enriched in carcinomas of many tiss ... | 1985 | 2410031 |
| activation of murine spleen cells by lipid a: negative modulation of lipid a mitogenic activity by o-antigen polysaccharide. | we have investigated the regulatory effects of polysaccharide-rich subunits upon lipid a activity with the use of hybrid lps macromolecules of defined subunit composition. hybrid lps were constructed with polysaccharide-rich lps from escherichia coli o55:b5 and lipid a-rich lps from salmonella minnesota r595 by dissociation of the two parental lps species to monomeric solutions with deoxycholate, admixing these lps in various proportions and reassociation into high m.w. lps hybrid aggregates by ... | 1985 | 2411800 |
| control of lipopolysaccharide-high-density lipoprotein interactions by an acute-phase reactant in human serum. | we have recently described several phenomena involving the interactions of lipopolysaccharides (lps) from salmonella minnesota re595 (re595-lps) with rabbit serum, which are different in and unique to acute-phase serum as compared with normal serum (p.s. tobias and r.j. ulevitch, j. immunol. 131:1913-1916, 1983). to determine whether these phenomena could also be observed in acute-phase human serum (aphs), we used aphs obtained from volunteers injected with etiocholanolone. as observed in acute- ... | 1985 | 2412965 |
| an enzyme-linked immunosorbent assay (elisa) for the measurement of antibodies to different parts of the gram-negative lipopolysaccharide core region. | bovine serum albumin was complexed with the core antigens of either escherichia coli j5 lps, salmonella minnesota r595 lps or e. coli lipid a. these core-bsa complexes were used for solid-phase coating in elisas for anti-core antibodies. antibodies, binding to various parts of the core region were easily quantified in a single experimental set-up, which was hitherto not possible. the elisa has only 3 incubation steps and is not costly as only moderate amounts of the core antigens (i.e., 1 microg ... | 1985 | 2413127 |
| cross-reactivity of rabbit antibodies to lipopolysaccharides of escherichia coli j5 and other gram-negative bacteria. | antiserum to rough gram-negative mutants such as escherichia coli j5 and salmonella minnesota re595 is thought to neutralize the toxic effects of lipopolysaccharides (lpss). to verify that such antisera are capable of binding heterologous endotoxins, we examined igg and igm class antibodies induced in rabbits to a variety of lpss. immunization with rough mutants or lipid a induced high igg antibody responses to the homologous purified lps and relatively low but significant responses to heterolog ... | 1985 | 2413146 |
| [use of immunoenzyme analysis for evaluating the specificity of the antibody recognition of the antigenic determinants of streptococcus group a]. | investigations carried out with the use of a celia system have revealed that antibodies in the sera of patients with primary erysipelas and antibodies in rabbit antiserum to the ribosomes of group a streptococcus specifically bind with adsorbed streptococcal ribosomes, recognizing the antigenic determinants of streptococcal ribosomes, which differ from those of individual gram-negative prokaryotes (escherichia coli, shigella sonnei, shigella flexneri, salmonella minnesota). the modified celia sy ... | 1985 | 2413662 |
| differences in attachment and phagocytosis of salmonella minnesota strains (s form, re mutant) by mouse peritoneal macrophages: participation of endogenous c1q and bacterial surface components (lps, porins). | | 1985 | 2417782 |
| chemiluminescence response of the human polymorphonuclear neutrophil to lipopolysaccharides. | polymorphonuclear neutrophils (pmn) respond to a variety of stimuli with a sequence of reactions that lead to the production of "active oxygen" species, including h2o2, free radicals, such as superoxide (o2-.) and hydroxyl (ho.), and singlet molecular oxygen (1o2). some of these can oxidize (5-amino-2,3-dihydrophthalazine 1,4-dione) (luminol) to the ground state aminophthalate ion; this reaction sequence is accompanied by the generation of a photon and forms the basis for the chemiluminescence ( ... | 1985 | 2420454 |
| structure-activity relationship of lipid a: comparison of biological activities of natural and synthetic lipid a's with different fatty acid compositions. | to investigate the structure-activity relationships, various biological activities, including pyrogenicity, lethal toxicity, elicitation of shwartzman reaction, mitogenicity and tumor necrosis factor (tnf)-inducing activity, were compared among natural and synthetic lipid a's differing in fatty acid composition. in all these tests, natural lipid a's from escherichia coli and salmonella minnesota and synthetic la-15-pp, which carries 3-hydroxy- and 3-acyloxy-tetradecanoyl groups at the 2, 3 and 2 ... | 1986 | 2423510 |
| the occurrence of anti-3-fucosyllactosamine antibodies and their cross-reactive idiotopes in preimmune and immune mouse sera. | the carbohydrate determinant 3-fucosyllactosamine (3-fl), gal(beta 1-4)[fuc alpha 1-3]glcnac-r, which is also known as ssea-1 or as the x determinant, is very immunogenic in balb/c mice. many monoclonal antibodies directed against this structure have been obtained by immunization of mice with murine teratocarcinomas and human carcinomas and myeloid cells. we have undertaken an analysis of the regulation of these antibodies and of their idiotypic, structural, and genetic diversity. we have descri ... | 1986 | 2430018 |
| staining of o-specific polysaccharide chains of lipopolysaccharides with ruthenium red. | s-form lipopolysaccharides (lps) from klebsiella strain len-1 (o3: k1-) and from salmonella minnesota strain 1114 were positively stained with ruthenium red, whereas r-form lps from klebsiella strain len-111 (o3-: k1-) and ra, rb1, rcp+, rd1p-, and re lps from the respective mutant strains of s. minnesota were not or only faintly stained by such treatment. from these results it was concluded that ruthenium red stains the o-specific polysaccharide chains of lps. the appearance of stained preparat ... | 1986 | 2431251 |
| use of synthetic antigens to determine the epitope specificities of monoclonal antibodies against the 3-deoxy-d-manno-octulosonate region of bacterial lipopolysaccharide. | mouse monoclonal antibodies were raised against heat-killed bacteria of the re mutant r595 of salmonella minnesota and characterized by the passive hemolysis and passive hemolysis inhibition tests and by double immunodiffusion experiments using lipopolysaccharide (lps) from different rough mutants of s. minnesota and synthetic antigens. the latter were copolymerization products of acrylamide with the alpha- and beta-allylglycosides of 3-deoxy-d-manno-octulosonic acid (kdo) and the alpha-2,4-link ... | 1987 | 2433221 |
| antigenic and immunogenic properties of recombinants from salmonella typhimurium and salmonella minnesota rough mutants expressing in their lipopolysaccharide a genus-specific chlamydial epitope. | rough mutants from salmonella typhimurium and salmonella minnesota were transformed with a plasmid containing a 6.5-kilobase insert of dna from chlamydia trachomatis assumed to encode a glycosyltransferase. transformation resulted in the expression of a genus-specific chlamydial epitope on the lipopolysaccharide (lps) of the recombinant strains. proteinase k-digested whole-cell lysates of the recombinants and of controls were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis ... | 1987 | 2433222 |
| acid-treated, naked bacteria as immune carriers for protein antigens. | salmonella minnesota r595 bacteria were stripped of their natural antigenic determinants to yield acid-treated, naked bacteria. the proteins, human apolipoprotein a1 and carcino-embryonic antigen, were adsorbed to naked bacteria and these complexes were used to immunise rabbits. although the antibody titres obtained were comparable to those achieved using freund's adjuvant emulsions, much less antigen was needed for immunisation. this technique could be of great value where the amount of protein ... | 1987 | 2437205 |
| contribution of the mannan o side-chains to the adjuvant action of lipopolysaccharides. | klebsiella 03 lipopolysaccharide (lps) and escherichia coli 09 lps were previously shown to have potent adjuvant activities in augmenting antibody response and delayed-type hypersensitivity to protein antigens. the r form lps extracted from the o-specific polysaccharide-less mutants derived from klebsiella 03 and e. coli 09 strains were characterized by chemical and electrophoretic analyses. the adjuvant activities of the r-lps in augmenting antibody response and dth to protein antigens were muc ... | 1987 | 2438205 |
| demonstration of cross-reactive antibodies to smooth gram-negative bacteria in antiserum to escherichia coli j5. | we investigated the discrepancy between the broad cross-protection against gram-negative infections afforded by antiserum to escherichia coli j5 and its apparently narrow cross-reactivity in vitro. rabbits immunized with j5 bacteria produced antibodies to both the j5 lipopolysaccharide (lps; titer by elisa, 1:60,000) and lps from the re mutant of salmonella minnesota (i.e., to the ketodeoxyoctonate [kdo] and lipid a determinants; titer, 1:3,200). in highly diluted antiserum, titers of antibody t ... | 1987 | 2439613 |
| failure of antisera to j5 and r595 rough mutants to reduce endotoxemic lethality. | rabbit antisera to j5 (rc) escherichia coli and r595 (re) salmonella minnesota rough mutants were selected for the highest content of hemagglutinating antibodies to their respective core glycolipids. despite titers of 1:2560 vs. j5 and 1:640 vs. r595 core glycolipids, the antisera failed to passively protect icr or cf-1 mice against lethality induced by endotoxins from a variety of wild-type, smooth enterobacteria: e. coli o111:b4, e. coli o127:b8, salmonella typhimurium, s. minnesota, and citro ... | 1988 | 2447200 |
| comparison of the opsonic activity of polyclonal and monoclonal antibodies raised against salmonella minnesota strain r595. | murine monoclonal antibodies and immune rabbit serum were raised against the rough mutant salmonella minnesota strain r595. these antibodies were tested for their opsonic activity against the homologous strain and the smooth wild type s. minnesota by luminol-dependent chemiluminescence and a microscopic assessment of phagocytosis. immune rabbit serum opsonised both strains. treatment with normal rabbit serum inhibited the phagocytic uptake of s. minnesota r595. none of the monoclonal antibodies ... | 1988 | 2448462 |
| identification of re lipopolysaccharide-binding protein on murine erythrocyte membrane. | our recent studies have suggested that bacterial lipopolysaccharide (lps) attaches to pronase-sensitive proteins on the murine erythrocyte membrane. in the present study, in order to identify the lps-binding protein on the murine erythrocyte membrane, a unique method to detect lps-binding protein on a nitrocellulose membrane was developed. murine erythrocyte membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, then transferred electrophoretically onto a ... | 1988 | 2453783 |
| characterization of the binding epitope of a monoclonal antibody to sulphatide. | an igg1 monoclonal antibody, sulph i, reacting with sulphatide (3'-sulphogalactosylceramide), was produced by immunizing balb/c mice with that glycolipid coated on salmonella minnesota bacterial membrane. radioimmunodetection of the binding of the monoclonal antibody to structurally related glycolipids adsorbed to microtitre plates or chromatographed on thin-layer plates was used to determine its binding epitope. the antibody showed similar binding avidity to three sulphated glycolipids: sulphat ... | 1988 | 2455508 |
| immunochemical specificity of human antibodies to lipopolysaccharide from the j5 rough mutant of escherichia coli o111:b4. | antibody to the core region of lipopolysaccharide (lps) of escherichia coli o111:b4 (j5) is reported to cross-react with lps from other gram-negative bacteria. we used an enzyme-linked immunosorbent assay to test various lpss and their constituents for their ability to inhibit binding of human igg antibody to j5 lps to j5 lps. results were expressed as the concentration of inhibitor required to cause 50% inhibition of binding (i50). i50 values of antibody to j5 lps for j5 lps ranged from 5 x 10( ... | 1989 | 2462603 |
| [specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants]. | the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ... | 1988 | 2464261 |
| prevention of lethal endotoxemia in actinomycin d-sensitized mice by incubation of salmonella minnesota r595 lipopolysaccharide with monoclonal antibodies to r595. | murine monoclonal antibodies reacting with lipopolysaccharide (lps) of salmonella minnesota strain r595 (re chemotype) were prepared, and tested for their ability to protect actinomycin d-sensitized mice against lethal endotoxemia. protection was found with some antibodies up to a 90-fold increase in ld50, whereas others exhibited no protection. the various protective antibodies did not all bind to the same epitope. the same applied for non-protective clones. protective and non-protective clones ... | 1988 | 2467166 |
| epitope specificities of murine monoclonal and rabbit polyclonal antibodies against enterobacterial lipopolysaccharides of the re chemotype. | murine monoclonal and rabbit polyclonal antibodies raised against the lipopolysaccharides (lps) of re mutants of salmonella minnesota, proteus mirabilis, and escherichia coli were serologically characterized. using natural re lps and natural and synthetic partial structures thereof, representing the 3-deoxy-d-manno-2-octulosonic acid (kdo) or lipid a region or both, the epitope specificities of four monoclonal antibodies were defined. clones 20 (immunoglobulin m [igm]) and 25 (igg3) recognize a ... | 1989 | 2474499 |
| effects of substance p on the spontaneous binding of salmonella minnesota r345 (rb) to human peripheral blood lymphocytes. | the effects of substance p (sp) on salmonella minnesota r345 (rb) binding to human peripheral blood lymphocytes (pbl) were evaluated. two parameters of bacterial cytoadherence were considered, namely the binding lymphocytes (bl) and the number of bound-bacteria/lymphocyte (bb). the results showed that sp inhibits both bl and bb in a significant manner. furthermore, distribution of salmonella binding to cd4+ and cd8+ lymphocytes was studied following sp pretreatment of lymphoid cells. this neurop ... | 1989 | 2482344 |
| structural and physicochemical requirements of endotoxins for the activation of arachidonic acid metabolism in mouse peritoneal macrophages in vitro. | lipopolysaccharides of different wild-type and mutant gram-negative bacteria, as well as synthetic and bacterial free lipid a, were studied for their ability to activate arachidonic acid metabolism in mouse peritoneal macrophages in vitro. it was found that lipopolysaccharides of deep-rough mutants of salmonella minnesota and escherichia coli (re to rc chemotypes) stimulated macrophages to release significant amounts of leukotriene c4 (ltc4) and prostaglandin e2 (pge2). lipopolysaccharides of wi ... | 1989 | 2492937 |
| effect of bacterial lipopolysaccharide on growth of murine bladder cancer, mbt-2. | intradermal palpable mbt-2 tumor responded with bacterial lipopolysaccharide to hemorrhagic necrosis (lps) in c3h/hen (endotoxin sensitive) mice. we have tested lps fractions isolated from e. coli, klebsiella pneumoniae, salmonella minnesota, pseudomonas aeruginosa and serratia culture filtrates. all these lps preparations showed tumor necrotizing activity accompanied by toxicity (body weight loss) in c3h/hen mice. however, mbt-2 tumors grown in an endotoxin-resistant strain (c3h/hej) of mice di ... | 1989 | 2510388 |
| role of c1q in phagocytosis of salmonella minnesota by pulmonary endothelial cells. | the re mutant of salmonella minnesota adheres in much greater numbers than the wild type to endothelial cells derived from the bovine pulmonary artery. since the re mutant is distinguished from wild-type s. minnesota by its ability to bind c1q and since endothelial cells possess receptors for c1q, we examined the role of c1q in the phagocytosis of the s. minnesota re mutant. first, preincubating endothelial cells with c1q-enriched medium resulted in increased adherence of the re mutant (17.9 x 1 ... | 1989 | 2540090 |
| gas chromatographic determination of (phosphorylated) 2-keto-3-deoxyoctonic acid, heptoses and glucosamine in bacterial lipopolysaccharides after treatment with hydrofluoric acid, methanolysis and trifluoroacetylation. | quantification of phosphorylated sugar constituents of lipopolysaccharides has been performed by the following sequence: dephosphorylation by treatment with hydrofluoric acid, cleavage to monomeric constituents by methanolysis and analysis of the released sugars by capillary gas chromatography. lipopolysaccharides of salmonella minnesota rd1p+, bordetella pertussis nih 114 and vibrio cholerae, nag and 95r strains, were used as model substances. comparison of the chromatographic data obtained fro ... | 1989 | 2541150 |
| the effect of bacterial lipopolysaccharide (lps) on histamine release from human basophils. i. enhancement of immunologic release by lps. | preincubation of human basophils with bacterial lipopolysaccharide (lps) purified from the heptose-deficient mutant salmonella minnesota r595 enhanced by an average of sixfold the response of peripheral blood basophils obtained from allergic donors to several allergens in vitro as judged by release of histamine. enhancement occurred at suboptimal, optimal, and supraoptimal concentrations of antigen. no effect was seen if basophils were from a nonallergic donor, and lps by itself rarely caused hi ... | 1985 | 2578909 |
| antigenic properties of chlamydia trachomatis lipopolysaccharide. | the antigenic properties of the lipopolysaccharide (lps) of chlamydia trachomatis l2 were investigated. by means of passive hemolysis, passive hemolysis inhibition, and absorption experiments, it was shown that antiserum raised against chlamydial elementary bodies contained at least two different antibody specificities which reacted with different antigenic determinants of chlamydial lps. one of these antibodies cross-reacted with enterobacterial re lps, recognizing a structure which is shared b ... | 1985 | 2580795 |
| binding activity of a murine anti-lipid a monoclonal antibody. | in this report we briefly describe an immunoglobulin g3 monoclonal antibody, 2g6/1h11, which binds purified lipid a from salmonella minnesota and a lipid a precursor molecule derived from salmonella typhimurium. 2g6/1h11 does not bind well to purified whole s. typhimurium lipopolysaccharide (lps), s. minnesota lps, lps preparations from a series of s. minnesota rough mutants, or intact s. typhimurium bacteria. thus, there are antigenic determinants in purified lipid a which are not exposed when ... | 1985 | 2580797 |
| [demonstration of a species specific determinant of bacteroides fragilis lipopolysaccharides using monoclonal antibodies]. | the reactivity of two monoclonal antibodies to bacteroides fragilis was tested (elisa) with 37 strains of b. fragilis, 1 strain each of b. thetaiotaomicron, b. ovatus, b. vulgatus and b. eggerthii, 1 strain of fusobacterium, as well as pseudomonas aeruginosa (6 strains), e coli (8 strains), klebsiella (2 strains), yersinia enterocolitica (1 strain) and lps from salmonella minnesota. both monoclonal antibodies proved to be specific for b. fragilis. the determinants recognized by these antibodies ... | 1985 | 2581371 |
| characterization of two different antibody specificities recognizing distinct antigenic determinants in free lipid a of escherichia coli. | antisera were raised in rabbits with acid-treated re mutant bacteria from salmonella minnesota and escherichia coli and tested in a passive hemolysis assay with di- and monophosphorylated free lipid a of e. coli (lipa-ac and lipa-hcl, respectively) coated onto sheep erythrocytes. depending on the acid used to prepare the immunogen (acetic versus hydrochloric acid), different antibody specificities were obtained. antiserum prepared against hcl-treated bacteria was found to react with both antigen ... | 1985 | 2581900 |
| temperature-dependent incorporation of 4-amino-l-arabinose in lipid a of distinct gram-negative bacteria. | the presence and the relative amount of 4-amino-l-arabinose in lipopolysaccharides of members of the enterobacteriaceae family and in a single strain of chromobacterium violaceum has been studied with regard to growth-temperature dependent variations. changes in the presence and the amount of 4-amino-l-arabinose (4-aa) were observed in almost all cases, but the variations observed were not consistent among different species. while salmonella minnesota and proteus mirabilis showed higher levels o ... | 1989 | 2583477 |
| human serum amyloid a protein. behaviour in aqueous and urea-containing solutions and antibody production. | human serum amyloid a protein (apo-saa) can be prepared by gel filtration of delipidated acute-phase high-density lipoprotein in the presence of urea. the resultant apo-saa is soluble (greater than 90% solubility) in a wide range of buffer solutions, with all of the six major isoforms of apo-saa being equally soluble. in urea-containing solutions the isoforms behave qualitatively differently in various urea concentrations, probably reflecting subtle primary-structure variations. the higher-pi is ... | 1989 | 2597108 |
| killing of gram-negative bacteria by complement. fractionation of cell membranes after complement c5b-9 deposition on to the surface of salmonella minnesota re595. | the effect of c5b-9 deposition on the envelope of target gram-negative bacteria was studied. in order to understand the changes occurring after complement deposition on the bacterial surface, the preparation of gram-negative bacterial membranes by different methods involving the osmotic lysis of spheroplasts was investigated. subsequent fractionation of the outer membrane (om) and cytoplasmic membrane (cm) by sucrose-density-gradient centrifugation showed differences in the membrane profiles obt ... | 1989 | 2597121 |
| an outbreak of food poisoning in a workers' camp in saudi arabia caused by salmonella minnesota. | between 13 and 16 september 1985, 168 of 419 filipino workers, living in a camp near dammam, saudi arabia, developed acute gastroenteritis with diarrhoea, vomiting, abdominal pain, and low-grade fever. the outbreak was confined to those who ate from a single kitchen. the median approximate incubation period of the disease was 34 hours (range 8 to 96 hours). salmonella minnesota was isolated from the stools and rectal swabs of 34% of the patients. one of the 27 cooks was positive for the organism ... | 1989 | 2607097 |
| gd3 expression by cultured human tumor cells of neuroectodermal origin. | seven monoclonal antibodies (mabs) reactive with ganglioside ii3(neuac)2-laccer (gd3) were generated; four of these mabs (dmab-21, dmab-22, dmab-23, and dmab-24) by immunizing mice with gd3 adsorbed to salmonella minnesota and the remaining three (dmab-7, dmab-8, and dmab-17) with melanoma line sk-mel 28, which contains 1.4 nmol sialic acid of gd3 per mg protein. the specificities of the mabs were defined by high-performance thin-layer chromatography (hptlc) immunostain and solid-phase radioimmu ... | 1989 | 2609939 |
| induction of tumor necrosis factor-alpha release by lipopolysaccharide and defined lipopolysaccharide partial structures. | we have investigated the release of tumor necrosis factor-alpha (tnf-alpha) by human mononuclear cells (mnc) and isolated human monocytes/macrophages stimulated with s- and r-form lipopolysaccharide (lps), natural lipid a, and natural and synthetic partial structures thereof. we found that lps of salmonella minnesota (s. min.) rb2, which represents a partial structure of wildtype lps of salmonella abortus equi (s.a.e.) lacking the o-chain and parts of the outer core region, was the most active i ... | 1989 | 2613271 |
| the production of highly specific anti-testosterone antisera using acid-treated bacteria as immunogenic carrier. | a novel and effective procedure for the production of highly specific anti-testosterone antibodies is described. it involved pretreatment of experimental animals with tolerogens followed by immunization with conjugates of testosterone covalently linked to acid-treated salmonella minnesota r595 bacteria as immunogenic carriers. antibodies elicited by this procedure showed minimal cross-reactivity towards 5 alpha-dihydrotestosterone and some of them were successfully used in radioimmunoassays for ... | 1989 | 2613285 |
| comparative x-ray and fourier-transform-infrared investigations of conformational properties of bacterial and synthetic lipid a of escherichia coli and salmonella minnesota as well as partial structures and analogues thereof. | by using the x-ray and infrared spectroscopic characteristics of various synthetic analogues and partial structures of lipid a in the dried state, a comparison of these compounds with their natural counterparts was undertaken. as judged by their x-ray diffraction and infrared spectroscopic features, the compounds tested could be divided into two main groups. the first group covered those samples synthesized in accordance with a previously assumed structure, while those synthesized in accordance ... | 1989 | 2646121 |
| il-1 induction-capacity of defined lipopolysaccharide partial structures. | natural and synthetic lipid a as well as natural and synthetic oligosaccharide partial structures of lps were examined in dose-response experiments to define the minimal structure necessary for il-1 induction and release in cultures of human mononuclear cells. wild type lps (s. abortus equi) and rough mutant lps was active in minimal-doses of 1 to 100 pg/ml, whereas synthetic heptaacyl and hexaacyl lipid a (salmonella minnesota and escherichia coli lipid a, respectively) induced il-1 in minimal- ... | 1989 | 2651523 |
| lipopolysaccharide (lps)-reactive monoclonal antibodies fail to inhibit lps-induced tumor necrosis factor secretion by mouse-derived macrophages. | murine monoclonal antibodies (mabs) reactive with epitopes on the o-side chain, core oligosaccharide, or lipid a of escherichia coli and salmonella minnesota lipopolysaccharide (lps) were evaluated for their ability to inhibit lps-induced tumor necrosis factor (tnf) secretion by mouse-derived raw 264.7 macrophages. as little as 50 ng of purified lps or lipid a stimulated macrophages to produce tnf detectable as cytotoxic activity in an l-929 fibroblast assay. none of 13 mabs (concentration range ... | 1989 | 2651532 |
| the effect of immunity to core lipopolysaccharides (lps) on the production of thromboxane and prostacyclin by equine peritoneal macrophages. | an experiment was designed to determine whether a change in the ability of macrophages to respond to lipopolysaccharides (lps) of gram-negative bacteria was involved in the development of cross-reactive immunity to endotoxemia. the endotoxin-induced production of thromboxane a2(txa2) and prostacyclin (pgi2) by peritoneal macrophages from horses which were hyperimmunized against the common core region of lps were compared to those in unimmunized horses. bacterins used for induction of core lps im ... | 1989 | 2666023 |
| evaluation of the opsonic capacity of core lipopolysaccharide antiserum of equine origin against smooth escherichia coli 0111:b4, using macrophage chemiluminescence. | a study was performed to determine whether equine antiserum to core lipopolysaccharide (lps) would enhance phagocytosis of smooth gram-negative (gn) organisms by equine macrophages. five healthy adult horses (group a) were immunized with a bacterin prepared from the j-5 mutant of escherichia coli 0111:b4 and salmonella minnesota r595 to produce antibodies to core lps. five horses (group b) served as nonimmunized controls and were given physiologic saline solution instead of the rough mutant bact ... | 1989 | 2675693 |
| isolation, cloning, and primary structure of a cationic 16-kda outer membrane protein of salmonella typhimurium. | by using acid-urea polyacrylamide gel electrophoresis, two cationic proteins were found in the isolated outer membranes of salmonella typhimurium sh5014. also, all the other enterobacterial strains studied (five additional strains of s. typhimurium, one strain of salmonella minnesota, and three strains of escherichia coli k12) had those proteins. the most abundant (omb2) was purified in preparative acid-urea polyacrylamide gel electrophoresis and reversed-phase high pressure liquid chromatograph ... | 1989 | 2681205 |
| cross-reactivity of monoclonal antibodies to escherichia coli j5 with heterologous gram-negative bacteria and extracted lipopolysaccharides. | a panel of eight murine monoclonal antibodies (mabs) were produced against heat-killed escherichia coli j5 and shown to react with j5 lipopolysaccharide (lps) by enzyme-linked immunosorbent assay (elisa). these antibodies were then assayed by a suspension elisa for reactivity with up to 20 heterologous smooth or rough isolates of gram-negative bacteria, which were assayed after heat or formalin treatment, or as live cells. extracted lps from the same bacteria were tested for reactivity with the ... | 1989 | 2681436 |