| cellulase with high β-glucosidase activity by penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue. | in this study, we investigated cellulase production by penicillium oxalicum eu2106 under solid-state fermentation (ssf) and its hydrolysis efficiency toward naoh-h2o2-pretreated cassava residue (nhcr) produced after bioethanol fermentation. optimization of ssf cultivation conditions for p. oxalicum eu2106 using a box-behnken design-based response-surface methodology resulted in maximal cellulase activity of 34.0 ± 2.8 filter-paper units/g dry substrate, exhibiting a ~ twofold increase relative t ... | 2017 | 28120306 |
| the different roles of penicillium oxalicum laea in the production of extracellular cellulase and β-xylosidase. | cellulolytic enzyme hydrolysis of lignocellulose biomass to release fermentable sugars is one of the key steps in biofuel refining. gene expression of fungal cellulolytic enzymes is tightly controlled at the transcriptional level. key transcription factors such as activator clrb/clr2 and xlnr/xyr1, as well as repressor crea/cre1 play crucial roles in this process. the putative protein methyltransferase laea/lae1 has also been reported to regulate the gene expression of the cellulolytic enzyme. t ... | 2016 | 28066400 |
| improving cellulase productivity of penicillium oxalicum re-10 by repeated fed-batch fermentation strategy. | medium optimization and repeated fed-batch fermentation were performed to improve the cellulase productivity by p. oxalicum re-10 in submerged fermentation. first, plackett-burman design (pbd) and central composite design (ccd) were used to optimize the medium for cellulase production. pbd demonstrated wheat bran and nano3 had significant influences on cellulase production. the ccd results showed the maximum filter paper activity (fpa) production of 8.61u/ml could be achieved in erlenmeyer flask ... | 2017 | 28013132 |
| evidence of gushing induction by penicillium oxalicum proteins. | the aim of this study was to elucidate whether grape-associated fungi exert an influence on gushing by their production of surface-active compounds. | 2017 | 27930840 |
| efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase from penicillium oxalicum. | starch is a very abundant and renewable carbohydrate and is an important feedstock for industrial applications. the conventional starch liquefaction and saccharification processes are energy-intensive, complicated, and not environmentally friendly. raw starch-digesting glucoamylases are capable of directly hydrolyzing raw starch to glucose at low temperatures, which significantly simplifies processing and reduces the cost of producing starch-based products. | 2016 | 27777618 |
| expression and chromatin structures of cellulolytic enzyme gene regulated by heterochromatin protein 1. | heterochromatin protein 1 (hp1, homologue hepa in penicillium oxalicum) binding is associated with a highly compact chromatin state accompanied by gene silencing or repression. hp1 loss leads to the derepression of gene expression. we investigated hepa roles in regulating cellulolytic enzyme gene expression, as an increasingly number of studies have suggested that cellulolytic enzyme gene expression is not only regulated by transcription factors, but is also affected by the chromatin status. | 2016 | 27729944 |
| comparative genomic, transcriptomic and secretomic profiling of penicillium oxalicum hp7-1 and its cellulase and xylanase hyper-producing mutant eu2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression. | the filamentous fungus penicillium oxalicum is a potential alternative to trichoderma reesei for industrial production of a complete cellulolytic enzyme system for a bio-refinery. comparative omics approaches can support rational genetic engineering and/or breeding of filamentous fungi with improved cellulase production capacity. in this study, comparative genomic, transcriptomic and secretomic profiling of p. oxalicum hp7-1 and its cellulase and xylanase hyper-producing mutant eu2106 were emplo ... | 2016 | 27688806 |
| oxad: a versatile indolic nitrone synthase from the marine-derived fungus penicillium oxalicum f30. | indole alkaloids are a diverse class of natural products known for their wide range of biological activities and complex chemical structures. rarely observed in this class are indolic nitrones, such as avrainvillamide and waikialoid, which possess potent bioactivities. herein the oxa gene cluster from the marine-derived fungus penicillium oxalicum f30 is described along with the characterization of oxad, a flavin-dependent oxidase that generates roquefortine l, a nitrone-bearing intermediate in ... | 2016 | 27505044 |
| putative methyltransferase laea and transcription factor crea are necessary for proper asexual development and controlling secondary metabolic gene cluster expression. | the morphological development of fungi is a complex process and is often coupled with secondary metabolite production. in this study, we assessed the function of putative methyltransferase laea and transcription factor crea in controlling asexual development and secondary metabolic gene cluster expression in penicillium oxalicum. the deletion of laea (δlaea) impaired the conidiation in p. oxalicum, with a downregulated expression of brla. overexpression of p. oxalicum brla in δlaea could upregul ... | 2016 | 27387217 |
| adsorption and mechanism of cellulase enzymes onto lignin isolated from corn stover pretreated with liquid hot water. | in the bioconversion of lignocellulosic substrates, the adsorption behavior of cellulase onto lignin has a negative effect on enzymatic hydrolysis of cellulose, decreasing glucose production during enzymatic hydrolysis, thus decreasing the yield of fermentation and the production of useful products. understanding the interaction between lignin and cellulase is necessary to optimize the components of cellulase mixture, genetically engineer high-efficiency cellulase, and reduce cost of bioconversi ... | 2016 | 27274766 |
| a new family-3 glycoside hydrolase from penicillium oxalicum bl 3005 catalyzing tyrosol glucosylation to form salidroside. | a glycoside hydrolase from penicillium oxalicum bl 3005 was purified to apparent homogeneity. its molecular mass was estimated to be 90 kda by sds-page. the enzyme was identified to be a new member of family-3 by peptide sequence. high transglycosylation activity was found in the hydrolytic reaction of cellobiose. in the reaction, salidroside (4-hydroxyphenethyl o-β-d-glucopyranoside) was formed by adding tyrosol as the glycosyl acceptor. the optimum reaction ph and temperature were ph 6.5 and 5 ... | 2016 | 27222303 |
| oxalicumone a, a new dihydrothiophene-condensed sulfur chromone induces apoptosis in leukemia cells through endoplasmic reticulum stress pathway. | oxalicumone a (poa1), a novel dihydrothiophene-condensed sulfur chromone isolated from the marine fungus penicillium oxalicum scsgaf 0023, showed cytotoxicity against several cancer cells previously. in this study, its anti-cancer activity and underlying mechanism of this action were investigated in leukemia cells like kg-1a, hl60, u937, and k562. the results showed that poa1 inhibited dose-/time-dependently cell growth and induced apoptosis in leukemia cells. also, poa1 caused cleavages of casp ... | 2016 | 27132813 |
| production of a high-efficiency cellulase complex via β-glucosidase engineering in penicillium oxalicum. | trichoderma reesei is a widely used model cellulolytic fungus, supplying a highly effective cellulase production system. recently, the biofuel industry discovered filamentous fungi from the penicillium genus as a promising alternative to t. reesei. | 2016 | 27034716 |
| a novel bzip transcription factor clrc positively regulates multiple stress responses, conidiation and cellulase expression in penicillium oxalicum. | cellulase production in filamentous fungi is largely regulated at the transcriptional level, and several transcription factors have been reported to be involved in this process. in this study, we identified clrc, a novel transcription factor in cellulase production in penicillium oxalicum. clrc and its orthologs have a highly conserved basic leucine zipper (bzip) dna binding domain, and their biological functions have not been explored. deletion of clrc resulted in pleiotropic effects, including ... | 2016 | 27012606 |
| proteomic analysis of the biomass hydrolytic potentials of penicillium oxalicum lignocellulolytic enzyme system. | the mining of high-performance enzyme systems is necessary to develop industrial lignocellulose bioconversion. large amounts of cellulases and hemicellulases can be produced by penicillium oxalicum. hence, the enzyme system of this hypercellulolytic fungus should be elucidated to help design optimum enzyme systems for effective biomass hydrolysis. | 2016 | 26997974 |
| bioweathering potential of cultivable fungi associated with semi-arid surface microhabitats of mayan buildings. | soil and rock surfaces support microbial communities involved in mineral weathering processes. using selective isolation, fungi were obtained from limestone surfaces of mayan monuments in the semi-arid climate at yucatan, mexico. a total of 101 isolates representing 53 different taxa were studied. common fungi such as fusarium, pestalotiopsis, trichoderma, and penicillium were associated with surfaces and were, probably derived from airborne spores. in contrast, unusual fungi such as rosellinia, ... | 2016 | 26941725 |
| cytotoxic 1,3-thiazole and 1,2,4-thiadiazole alkaloids from penicillium oxalicum: structural elucidation and total synthesis. | two new thiazole and thiadiazole alkaloids, penicilliumthiamine a and b (2 and 3), were isolated from the culture broth of penicillium oxalicum, a fungus found in acrida cinerea. their structures were elucidated mainly by spectroscopic analysis, total synthesis and x-ray crystallographic analysis. biological evaluations indicated that compound 1, 3a and 3 exhibit potent cytotoxicity against different cancer cell lines through inhibiting the phosphorylation of akt/pkb (ser 473), one of important ... | 2016 | 26927049 |
| a novel acid-stable endo-polygalacturonase from penicillium oxalicum cz1028: purification, characterization, and application in the beverage industry. | acidic endo-polygalacturonases are the major part of pectinase preparations and extensively applied in the clarification of fruits juice, vegetables extracts, and wines. however, most of the reported fungal endo-polygalacturonases are active and stable under narrow ph range and low temperatures. in this study, an acidic endo-polygalacturonase (epg4) was purified and characterized from a mutant strain of penicillium oxalicum. the n-terminal amino acid sequence of epg4 (attctfsgsngaasasksqt) was d ... | 2016 | 26907758 |
| a biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse. | in this study, a co-production of two high value-added products, glucose and xylooligosaccharides (xos), was investigated by utilizing sugarcane bagasse (sb) within a multi-product bio-refinery framework optimized by box-behnken design-based response surface methodology. the developed process resulted in a maximum cellulose conversion of xylan-removed sb, 98.69±1.30%, and a maximum extracted sb xylan conversion into xos (xylobiose and xylotriose) of 57.36±0.79% that was the highest sb xylan conv ... | 2016 | 26773956 |
| penicillium oxalicum poflbc regulates fungal asexual development and is important for cellulase gene expression. | filamentous fungi can initiate vegetative growth on complex plant polysaccharides in nature through secreting a large amount of lignocellulose-degrading enzymes. these fungi develop a large amount of asexual spores to disperse and survive under harsh conditions, such as carbon and nitrogen depletion. numerous studies report the presence of a cross-talk between asexual development and extracellular enzyme production, especially at the regulation level. this study identified and characterized a c2 ... | 2016 | 26724278 |
| a new phenolic enamide and a new meroterpenoid from marine alga-derived endophytic fungus penicillium oxalicum en-290. | ten secondary metabolites (1-10) including a new phenolic enamide, methyl (z)-3-(3,4-dihydroxyphenyl)-2-formamidoacrylate (1), and a new meroterpenoid, 15-hydroxydecaturin a (2), were characterized from the etoac extracts of the marine alga-derived endophytic fungus penicillium oxalicum en-290. the structures of these compounds were elucidated on the basis of 1d and 2d nmr experiments and the absolute configuration of compound 2 was confirmed by electronic circular dichroism quantum calculations ... | 2015 | 26670415 |
| the development of genetic and molecular markers to register and commercialize penicillium rubens (formerly penicillium oxalicum) strain 212 as a biocontrol agent. | penicillium oxalicum strain 212 (po212) is an effective biocontrol agent (bca) against a large number of economically important fungal plant pathogens. for successful registration as a bca in europe, po212 must be accurately identified. in this report, we describe the use of classical genetic and molecular markers to characterize and identify po212 in order to understand its ecological role in the environment or host. we successfully generated pyrimidine (pyr-) auxotrophic mutants. in addition w ... | 2016 | 26467970 |
| isocoumarins from the fermentation products of a plant entophytic fungus penicillium oxalicum. | three new isocoumarins, terrecoumarins a-c (1-3), together with six known isocoumarins (4-9) were isolated from the fermentation products of the fungus penicillium oxalicum 0403. their structures were elucidated by spectroscopic methods, including extensive 1d and 2d nmr techniques. the anti-tobacco mosaic virus (anti-tmv) activities of 1-9 were evaluated. the results revealed that compound 1 showed high anti-tmv activity with inhibition rate 25.4 ± 3.5%. other compounds also showed weak activit ... | 2015 | 26411648 |
| a newly isolated penicillium oxalicum 16 cellulase with high efficient synergism and high tolerance of monosaccharide. | compared to trichoderma reesei rut-c30 cellulase (trcel), penicillium oxalicum 16 cellulase (p16cel) from the fermentation supernatant produced a 2-fold higher glucose yield when degrading microcrystalline cellulose (mcc), possessed a 10-fold higher β-glucosidase (bgl) activity, but obtained somewhat lower other cellulase component activities. the optimal temperature and ph of β-1,4-endoglucanase, cellobiohydrolase, and filter paperase from p16cel were 50-60 °c and 4-5, respectively, but those o ... | 2016 | 26410224 |
| purification, sequencing and evaluation of a divergent phytase from penicillium oxalicum kctc6440. | a fungal strain producing high levels of phytase was purified to homogeneity from penicillium oxalicum kctc6440 (phya). the molecular mass of the purified phya was 65 kda and optimal activity occurred at 55°c. the enzyme was stable in a ph range of 4.5-6.5, with an optimum performance at ph 5.5. the km value for the substrate sodium phytate was 0.48 mm with a vmax of 672 u/mg. the enzyme was inhibited by ca(2+), cu(2+), and zn(2+), and slightly enhanced by edta. the phya efficiently released pho ... | 2015 | 26377131 |
| synergistic and dose-controlled regulation of cellulase gene expression in penicillium oxalicum. | filamentous fungus penicillium oxalicum produces diverse lignocellulolytic enzymes, which are regulated by the combinations of many transcription factors. here, a single-gene disruptant library for 470 transcription factors was constructed and systematically screened for cellulase production. twenty transcription factors (including clrb, crea, xlnr, ace1, amyr, and 15 unknown proteins) were identified to play putative roles in the activation or repression of cellulase synthesis. most of these re ... | 2015 | 26360497 |
| functional diversity and properties of multiple xylanases from penicillium oxalicum gz-2. | a multiple xylanase system with high levels of xylanase activity produced from penicillium oxalicum gz-2 using agricultural waste as a substrate has been previously reported. however, the eco-physiological properties and origin of the multiplicity of xylanases remain unclear. in the present study, eight active bands were detected using zymography, and all bands were identified as putative xylanases using maldi-tof-ms/ms. these putative xylanases are encoded by six different xylanase genes. to ev ... | 2015 | 26224514 |
| function of a p24 heterodimer in morphogenesis and protein transport in penicillium oxalicum. | the lignocellulose degradation capacity of filamentous fungi has been widely studied because of their cellulase hypersecretion. the p24 proteins in eukaryotes serve important functions in this secretory pathway. however, little is known about the functions of the p24 proteins in filamentous fungi. in this study, four p24 proteins were identified in penicillium oxalicum. six p24 double-deletion strains were constructed, and further studies were carried out with the δerpδpδ strain. the experimenta ... | 2015 | 26149342 |
| optimization of cellulase production by penicillium oxalicum using banana agrowaste as a substrate. | the purpose of this study was to produce a higher amount of cellulase by using an alternative carbon source, such as banana agrowaste, and to optimize the fermentation parameters for a high yield. in the present study, cellulase-producing penicillium was isolated from a decaying wood sample. different nutritional and environmental factors were investigated to assess their effect on cellulase production. the highest crude enzyme production was observed at a ph 6.0 and a temperature of 28°c in a m ... | 2015 | 26018499 |
| new compound with dna topo i inhibitory activity purified from penicillium oxalicum hsy05. | strain hsy05 was isolated from sea sediment collected from the south china sea and was later identified as penicillium oxalicum by 16s rdna sequence analysis. various chromatographic processes led to the isolation and purification of two metabolites from the fermentation culture of hsy05, including one new compound, 2,2',4,4'-tetrahyoxy-8'-methyl-6-methoxy-acyl-ethyl-diphenylmethanone (1), and a known compound secalonic acid d (sad, 2), as characterised by uv, ir, 1d, 2d-nmr and ms data. the inh ... | 2015 | 25966868 |
| redesigning the regulatory pathway to enhance cellulase production in penicillium oxalicum. | in cellulolytic fungi, induction and repression mechanisms synchronously regulate the synthesis of cellulolytic enzymes for accurate responses to carbon sources in the environment. many proteins, particularly transcription regulatory factors involved in these processes, were identified and genetically engineered in penicillium oxalicum and other cellulolytic fungi. despite such great efforts, its effect of modifying a single target to improve the production of cellulase is highly limited. | 2015 | 25949521 |
| efficient production and evaluation of lignocellulolytic enzymes using a constitutive protein expression system in penicillium oxalicum. | native lignocellulolytic enzyme systems secreted by filamentous fungi can be further optimized by protein engineering or supplementation of exogenous enzyme components. we developed a protein production and evaluation system in cellulase-producing fungus penicillium oxalicum. first, by deleting the major amylase gene amy15a, a strain δ15a producing few extracellular proteins on starch was constructed. then, three lignocellulolytic enzymes (bgl4, xyn10b, and cel12a) with originally low expression ... | 2015 | 25868624 |
| linker length and flexibility induces new cellobiohydrolase activity of pocel6a from penicillium oxalicum. | in a previous study, a novel cellobiohydrolase, pocel6a, with new enzymatic activity against p-nitrophenyl-β-d-cellobioside (pnpc), was purified from penicillium oxalicum. the cellulose-binding module and catalytic domain of pocel6a showed a high degree of sequence similarity with other fungal cel6as. however, pocel6a had 11 more amino acids in the linker region than other cel6as. to evaluate the relationship between the longer linker of pocel6a and its enzymatic activity, 11 amino acids were de ... | 2015 | 25866282 |
| voriconazole-resistant penicillium oxalicum: an emerging pathogen in immunocompromised hosts. | penicillium species are rarely reported agents of infections in immunocompromised patients. we report 3 cases of invasive mycosis caused by voriconazole-resistant penicillium oxalicum in patients with acute myeloid leukemia, diabetes mellitus, and chronic obstructive pulmonary disease, while on voriconazole therapy. penicillium oxalicum has not been previously recognized as a cause of invasive mycoses. | 2014 | 25734109 |
| butyrolactones derivatives from the fermentation products of a plant entophytic fungus penicillium oxalicum. | two new butyrolactone derivatives, terrelactones a and b (1 and 2), together with four known butyrolactone derivatives (3-6), were isolated from the fermentation products of the fungus penicillium oxalicum 0403. their structures were elucidated by spectroscopic methods, including extensive 1d and 2d nmr techniques. the anti-tobacco mosaic virus (anti-tmv) activities of 1 and 2 were evaluated. the results showed that compounds 1 and 2 exhibited high anti-tmv activity with inhibition rates of 25.5 ... | 2015 | 25702805 |
| enhanced cellulase production by penicillium oxalicum for bio-ethanol application. | present study was focused on cellulase production from an indigenously isolated filamentous fungal strain, identified as penicillium oxalicum. initially, cellulase production under submerged fermentation in shake flasks resulted in cellulase activity of 0.7 fpu/ml. optimization of process parameters enhanced cellulase production by 1.7-fold and resulted in maximum cellulase activity of 1.2 fpu/ml in 8 days. cellulase production was successfully scaled-up to 7 l fermenter under controlled conditi ... | 2015 | 25661515 |
| newly isolated penicillium oxalicum a592-4b secretes enzymes that degrade milled rice straw with high efficiency. | an enzyme producing micro-organism, which can directly saccharify rice straw that has only been crushed without undergoing the current acid or alkaline pretreatment, was found. from the homology with the its, 28s rdna sequence, the strain named a592-4b was identified as penicillium oxalicum. activities of the a592-4b enzymes and commercial enzyme preparations were compared by novozymes cellic ctec2 and genencore gc220. in the present experimental condition, activity of a592-4b enzymes was 2.6 ti ... | 2015 | 25586551 |
| endophytes in the plant huperzia serrata: fungal diversity and discovery of a new pentapeptide. | endophytic fungi are an underexploited resource of natural products and have a capacity to produce diverse classes of plant-derived secondary metabolites. here, we investigated the diversity of endophytic fungi from huperzia serrata and the potential for discovering novel fungal natural products. one hundred and fifty-five endophytic fungi isolates obtained from h. serrata, belonging to four classes dothideomycetes (47.3 %), sordariomycetes (36.8 %), eurotiomycetes (10.6 %) and an undefined clas ... | 2015 | 25526853 |
| insights into high-efficiency lignocellulolytic enzyme production by penicillium oxalicum gz-2 induced by a complex substrate. | agricultural residue is more efficient than purified cellulose at inducing lignocellulolytic enzyme production in penicillium oxalicum gz-2, but in trichoderma reesei rut-c30, cellulose induces a more efficient response. to understand the reasons, we designed an artificially simulated plant biomass (cellulose plus xylan) to study the roles and relationships of each component in the production of lignocellulolytic enzymes by p. oxalicum gz-2. | 2014 | 25419234 |
| transformation of inorganic p fractions of soil and plant growth promotion by phosphate-solubilizing ability of penicillium oxalicum i1. | the solubilization of tricalcium phosphate is often considered as the standard for screening of most phosphate-solubilizing microorganisms (psms). however, usually the effect of large-scale application of psm on the promotion of crop growth varies. this study presents an efficient method for screening and testing phosphate-solubilizing fungus that enhance plant growth. a fungus penicillium oxalicum i1 (p-i1) was isolated and identified that had high ability of phosphate-solubilization and could ... | 2014 | 25363630 |
| a new acidophilic thermostable endo-1,4-β-mannanase from penicillium oxalicum gz-2: cloning, characterization and functional expression in pichia pastoris. | endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1, 4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and has a number of applications in different biotechnology industries. penicillium oxalicum is a powerful hemicellulase-producing fungus (bioresour technol 123:117-124, 2012); however, few previous studies have focused on the cloning and expression of the endo-1,4-β-mannanase gene from penicillium oxalicum. | 2014 | 25348022 |
| differential expression of peroxidase and abc transporter as the key regulatory components for degradation of azo dyes by penicillium oxalicum sar-3. | fungal species are potential dye decomposers since these secrete spectra of extracellular enzymes involved in catabolism. however, cellular mechanisms underlying azo dye catalysis and detoxification are incompletely understood and obscure. a potential strain designated as penicillium oxalicum sar-3 demonstrated broad-spectrum catabolic ability of different azo dyes. a forward suppression subtractive hybridization (ssh) cdna library of p. oxalicum sar-3 constructed in presence and absence of azo ... | 2014 | 25270890 |
| characterization, gene cloning, and sequencing of a fungal phytase, phya, from penicillium oxalicum pj3. | a phytase from penicillium oxalicum pj3, phya, was purified near to homogeneity with 427-fold increase in specific phytase activity by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatographies. sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and zymogram analysis of the purified enzyme indicated an estimated molecular mass of 65 kd. the optimal ph and temperature of the purified enzyme were ph 4.5 and 55°c, respectively. the enzyme activity was strong ... | 2015 | 24839991 |
| a new acidophilic endo-β-1,4-xylanase from penicillium oxalicum: cloning, purification, and insights into the influence of metal ions on xylanase activity. | a new acidophilic xylanase (xyn11a) from penicillium oxalicum gz-2 has been purified, identified and characterized. synchronized fluorescence spectroscopy was used for the first time to evaluate the influence of metal ions on xylanase activity. the purified enzyme was identified by maldi tof/tof mass spectrometry, and its gene (xyn11a) was identified as an open reading frame of 706 bp with a 68 bp intron. this gene encodes a mature protein of 196 residues with a predicted molecular weight of 21. ... | 2014 | 24818699 |
| dihydrothiophene-condensed chromones from a marine-derived fungus penicillium oxalicum and their structure-bioactivity relationship. | four dihydrothiophene-condensed chromones including two new compounds oxalicumones d-e (1-2) and known oxalicumones a-b (3-4), along with five other known chromones were isolated from a culture broth of the marine gorgonian-associated fungus penicillium oxalicum scsgaf 0023. the structures of 1-2 were elucidated by spectroscopic analysis. eleven derivatives 3a-3i and 4a-4b were obtained from the acylation of 3 and 4, respectively. compounds 1-4, 3a-3e, 3g-3h, and 4b showed significant cytotoxici ... | 2014 | 24767845 |
| functional characterization of protein kinase ck2 regulatory subunits regulating penicillium oxalicum asexual development and hydrolytic enzyme production. | casein kinase ck2 is a ubiquitous and conserved phosphate transferase that is critical for the growth and development of eukaryotic cells. in penicillium oxalicum, one catalytic subunit (ck2a) and two regulatory subunits (ck2b1 and ck2b2) of ck2 were annotated. in this study, ck2 regulatory subunit-defective mutants δck2b1 and δck2b2 were constructed to investigate the biological function of ck2 in p. oxalicum. the δck2b1 strain exhibited minimal changes in morphogenesis and conidiation, whereas ... | 2014 | 24613994 |
| a novel bifunctional pectinase from penicillium oxalicum sx6 with separate pectin methylesterase and polygalacturonase catalytic domains. | a multimodular pectinase of glycoside hydrolase family 28, s6a, was identified in penicillium oxalicum sx6 that consists of an n-terminal catalytic domain of pectin methylesterase, a thr/ser-rich linker region, and a c-terminal catalytic domain of polygalacturonase. recombinant s6a and its two derivatives, s6pe (the catalytic domain of pectin methylesterase) and s6pg (the catalytic domain of polygalacturonase), were produced in pichia pastoris. s6a was a bifunctional protein and had both pectin ... | 2014 | 24584459 |
| gymnemagenin-producing endophytic fungus isolated from a medicinal plant gymnema sylvestre r.br. | gymnema sylvestre is a plant containing the triterpenoid gymnemagenin, which is used in the pharmaceutical industry as an antidiabetic agent. the objective of this study was to determine whether endophytic fungi, isolated from g. sylvestre, produce gymnemagenin. we isolated an endophytic fungal strain from the leaves of g. sylvestre which produces gymnemagenin in the medium. the fungus was identified as penicillium oxalicum based on morphological and molecular methods. the strain had a component ... | 2014 | 24497046 |
| biodegradation of azo dyes acid red 183, direct blue 15 and direct red 75 by the isolate penicillium oxalicum sar-3. | soils contaminated with dyes were collected and screened for obtaining potential fungal strains for the degradation of azo dyes. a strain that demonstrated broad spectrum ability for catabolizing different azo dyes viz. acid red 183 (ar 183), direct blue 15 (db 15) and direct red 75 (dr 75) at 100 mg l(-1) concentration was subsequently identified as penicillium oxalicum sar-3 based on 18s and internal transcribed spacer (its) rdna gene sequence analysis. the strain has shown remarkably higher l ... | 2014 | 24418068 |
| enhanced production of chitin deacetylase by penicillium oxalicum saem-51 through response surface optimization of fermentation conditions. | optimization of the fermentation conditions for chitin deacetylase (cda) production by penicillium oxalicum saem-51 was undertaken in the present study using central composite design (ccd) under submerged condition. cda is widely employed for bio-catalytic conversion of chitin to chitosan. chitosan is a biopolymer with immense commercial potential in diverse industrial sectors, viz. pharmaceutics, food, agriculture, water treatment, etc. cda production was significantly affected by all the varia ... | 2014 | 28324456 |
| novel decaturin alkaloids from the marine-derived fungus penicillium oxalicum. | from the mycelia of penicillium oxalicum two new compounds, decaturins e (1) and f (2), have been isolated, along with four known analogues, decaturin a (3), decaturin c (4), decaturin d (5), and oxalicine b (6). the structures were determined by hr-esi-ms and 1d and 2d nmr analysis. | 2013 | 24354183 |
| the distinctive regulatory roles of prtt in the cell metabolism of penicillium oxalicum. | prtt is a fungal-specific transcription activator of extracellular proteases in aspergilli. in this study, the roles of the prtt homolog from penicillum oxalicum was investigated by transcription profiling in combination with electrophoretic mobility shift assay (emsa). the prtt deletion dramatically reduced extracellular protease activities and caused intracellular nutrient limitation when cultured on casein as the sole carbon source. prtt was found to directly regulate the expression of an int ... | 2014 | 24333140 |
| two new compounds from a marine-derived fungus penicillium oxalicum. | two new anthraquinones, emodin-3-o-sulphate (1) and citreorosein-3-o-sulphate (2), as well as five known anthraquinones, were isolated from a marine-derived fungus penicillium oxalicum. the structures of these compounds were determined by spectroscopic methods (1d and 2d nmr, hr-esi-ms). | 2014 | 24261577 |
| cellodextrin transporters play important roles in cellulase induction in the cellulolytic fungus penicillium oxalicum. | cellodextrin transporters (cellodextrin permeases) have been identified in fungi in recent years. however, the functions of these transporters in cellulose utilization and cellulase expression have not been well studied. in this study, three cellodextrin transporters, namely, cdtc, cdtd, and cdtg, in the cellulolytic fungus penicillium oxalicum (formally was classified as p. decumbens) were identified, and their functions were analyzed. the deletion of a single cellodextrin transporter gene slig ... | 2013 | 24132667 |
| enhanced production of a novel cytotoxic chromone oxalicumone a by marine-derived mutant penicillium oxalicum scsio 24-2. | many marine natural products hold great potential for the development of new and much needed drugs. however, the production of active metabolites by marine-derived microorganisms is usually very low, and large-scale culture has to be involved to meet the need of chemical structural modification and deep pharmacy study. in order to enhance the production of a novel cytotoxic sulfur-containing chromone oxalicumone a (oa), germinating spores of a marine-derived wild strain penicillium oxalicum scsg ... | 2013 | 24061416 |
| cytotoxic dihydrothiophene-condensed chromones from the marine-derived fungus penicillium oxalicum. | two new dihydrothiophene-condensed chromones and a new natural chromone, namely oxalicumones a-c (1-3), respectively, were isolated from a culture broth of a marine-derived fungus, penicillium oxalicum. the structures of 1-3 and acetylated derivatives of 1 (4-7) were elucidated on the basis of spectroscopic methods and chemical reactions. the absolute configuration of 1 and 2 were established by using the modified mosher ester method and circular dichroism data of an in situ formed [rh2(ococf3)4 ... | 2013 | 24037588 |
| a novel and other bioactive secondary metabolites from a marine fungus penicillium oxalicum 0312f1. | a new compound 2-(4-hydroxybenzoyl) quinazolin-4(3h)-one (1) and four known compounds were isolated from a marine fungus penicillium oxalicum 0312f1. the structure of the new compound 1 was elucidated based on the spectroscopic analysis. bioactivity assays showed that 2-(4-hydroxybenzyl) quinazolin-4(3h)-one (2) and methyl 4-hydroxyphenylacetate (5) had potent inhibitory activity against tobacco mosaic virus (tmv), and the new compound 1 had moderate inhibitory activity against tmv and human gas ... | 2013 | 23962399 |
| evolution of microbial aerosol behaviour in heating, ventilating and air-conditioning systems--quantification of staphylococcus epidermidis and penicillium oxalicum viability. | the aim of this study was to develop an experimental set-up and a methodology to uniformly contaminate several filter samples with high concentrations of cultivable bacteria and fungi. an experimental set-up allows contaminating simultaneously up to four filters for range of velocities representative of heating, ventilating and air-conditioning systems. the test aerosol was composed of a microbial consortium of one bacterium (staphylococcus epidermidis) and one fungus (penicillium oxalicum) and ... | 2013 | 23837350 |
| bioconversion to chitosan: a two stage process employing chitin deacetylase from penicillium oxalicum saem-51. | chitin deacetylase from penicillium oxalicum saem-51 was evaluated for bioconversion of chitin to chitosan in a two stage chemical and enzymatic process. variations in morphology, crystallinity and thermal properties following chemical treatment were evaluated by scanning electron microscopy, x-ray diffraction, differential scanning calorimetry and thermogravimetric analysis. degree of deacetylation of the substrates was determined using ft-ir and elemental analysis. the pretreatment of substrat ... | 2013 | 23768582 |
| characterization of a novel swollenin from penicillium oxalicum in facilitating enzymatic saccharification of cellulose. | plant expansins and fungal swollenin that can disrupt crystalline cellulose have great potential for applications in conversion of biomass. recent studies have been mainly focused on trichoderma reesei swollenin that show relatively low activity in the promotion of cellulosic hydrolysis. our aim was to isolate a novel swollenin with greater disruptive activity, to establish an efficient way of producing recombinant swollenin, and to optimize the procedure using swollenin in facilitation of cellu ... | 2013 | 23688024 |
| enhancement of supercritical co2 inactivation of spores of penicillium oxalicum by ethanol cosolvent. | the inactivation of spores of penicillium oxalicum by supercritical carbon dioxide (sc-co2) was optimized by response surface methodology. the optimal inactivation conditions of 16.8 mpa, 49°c, and 20 min were determined using ridge analysis, at which the predicted and experimental log10 reductions were obtained as 5.74 and 6.12, respectively. the synergistic effect of a cosolvent (ethanol), which was used to modify sc-co2, on the inactivation of the fungal spores was investigated. at less sever ... | 2013 | 23676914 |
| molecular cloning and heterologous expression of an acid-stable endoxylanase gene from penicillium oxalicum in trichoderma reesei. | an endoxylanase gene (poxyna) that belongs to the glycoside hydrolase (gh) family 11 was cloned from a xylanolytic strain, penicillium oxalicum b3-11(2). poxyna was overexpressed in trichoderma reesei qm9414 by using a constitutive strong promoter of the encoding pyruvate decarboxylase (pdc). the high extracellular xylanase activities in the fermentation liquid of the transformants were maintained 29~35-fold higher compared with the wild strain. the recombinant poxyna was purified to homogeneity ... | 2013 | 23412069 |
| cytotoxic dihydrothiophene-condensed chromones from marine-derived fungus penicillium oxalicum. | two new dihydrothiophene-condensed chromones and a new natural chromone, namely oxalicumones a-c (1-3), respectively, were isolated from a culture broth of a marine-derived fungus penicillium oxalicum scsgaf 0023, meripilaceae family. the structures of 1-3 and acetylated derivatives of 1 (4-7) were elucidated on the basis of spectroscopic methods and chemical reactions. the absolute configuration of 1 was established by using the modified mosher ester method and circular dichroism data of in sit ... | 2012 | 23150074 |
| production and characterization of acidophilic xylanolytic enzymes from penicillium oxalicum gz-2. | multiple acidophilic xylanolytic enzymes were produced by penicillium oxalicum gz-2 during growth on wheat straw, rice straw, corn stover, and wheat bran. the expression of xylanase isoforms was dependent on substrate type and nitrogen source. the zymograms produced by the sds-page resolution of the crude enzymes indicated that wheat straw was the best inducer, resulting in the highest xylanase (115.2u/ml) and β-xylosidase (89mu/ml) activities during submerged fermentation. the optimum temperatu ... | 2012 | 22940308 |
| biodegradation of poly(ε-caprolactone) (pcl) by a new penicillium oxalicum strain dsyd05-1. | in this study, fungi isolated from soil were screened for their ability to form clear zones on agar plates with emulsified poly(ε-caprolactone) (pcl). the most active strain, designated as dsyd05, was identified as penicillium oxalicum on the basis of morphological characteristics and phylogenetic analysis. mutant dsyd05-1, obtained by ultraviolet-light mutagenesis from strain dsyd05, was more effective in pcl degradation. in liquid cultures of the mutant strain with pcl emulsion, dsyd05-1 showe ... | 2012 | 22806733 |
| mass spectrometry-based chemotaxonomic classification of penicillium species (p. echinulatum, p. expansum, p. solitum, and p. oxalicum) and its correlation with antioxidant activity. | in this study, 4 penicillium species (17 strains) were classified on the basis of metabolite profile (chemotaxonomy) by using liquid chromatography-electrospray ionization ion trap-mass spectrometry (lc-esi-ms), gas chromatography-ion trap-mass spectrometry (gc-it-ms) and multivariate statistical analysis. the lc-esi-ms-based dendrogram was similar to the internal transcribed spacer (its)-based dendrogram, in that penicillium oxalicum was separated from the other 3 species. moreover, vermiculidi ... | 2012 | 22732319 |
| disinfection of wheat grains contaminated with penicillium oxalicum spores by a supercritical carbon dioxide-water cosolvent system. | spores of the plant pathogenic fungus penicillium oxalicum inoculated onto wheat grains were inactivated using supercritical carbon dioxide (sc-co(2)). after the sc-co(2) treatment at various conditions of temperature, time and amount of water cosolvent, the colony forming units (cfu) of fungal spores on wheat grains and the germination yields of wheat grains were determined. among these sc-co(2) treatment parameters, the inactivation of p. oxalicum spores was found to be significantly increased ... | 2012 | 22551673 |
| synthesis, photocatalytic, and antifungal properties of mgo, zno and zn/mg oxide nanoparticles for the protection of calcareous stone heritage. | more recently, the biological colonization of stone heritage and consequently its biodeterioration has become the focus of numerous studies. among all microorganisms, fungi are considered to be one of the most important colonizers and biodegraders on stone materials. this is why the development of new antifungal materials requires immediate action. znmgo nanoparticles (nps) have several exciting applications in different areas, highlighting as an efficient antimicrobial agent for medical applica ... | 2017 | 28679041 |
| constitutive expression of chimeric transcription factors enables cellulase synthesis under non-inducing conditions in penicillium oxalicum. | industrial production of cellulase by filamentous fungi is largely dependent on cellulose, which serves as a natural inducer of cellulase expression. however, insoluble cellulose is unfavorable to submerged fermentation and thus limits the production level of cellulase. here, the possibility of cellulase production under non-inducing conditions was explored in penicillium oxalicum by overexpressing two chimeric transcription factors. the chimeric transcription factors contain the dna binding dom ... | 2017 | 28755483 |
| isolation of limonoid compound (hamisonine) from endophytic fungi penicillium oxalicum la-1 (kx622790) of limonia acidissima l. for its larvicidal efficacy against lf vector, culex quinquefasciatus (diptera: culicidae). | upon screening for novel and potential biocompounds with larvicidal activities, we successfully isolated hamisonine (hmsn) a limonoid compound from endophytic fungi penicillium oxalicum la-1 of limonia acidissima. the extracted compound structure was elucidated by spectral studies such as uv-vis spectroscopy, thin-layer chromatography, ftir, lc-esi-ms, (1)h nmr, and (13)c nmr upon comparing with the spectral data available in the literature. further, the isolated hmsn was tested against iii and ... | 2017 | 28741206 |
| production of sodium gluconate from delignified corn cob residue by on-site produced cellulase and co-immobilized glucose oxidase and catalase. | the production of sodium gluconate by enzymatic catalysis of delignified corn cob residue (dccr) hydrolysate was studied. penicillium oxalicum i1-13 was used for the production of cellulase with high β-glucosidase activity. a fed-batch saccharification process was developed to obtain high yields of glucose. at the end of hydrolysis, the concentration of glucose reached 145.80g/l. glucose oxidase and catalase were co-immobilized to catalyze dccr hydrolysate to produce sodium gluconate. under the ... | 2017 | 28716292 |
| improvement of cellulolytic enzyme production and performance by rational designing expression regulatory network and enzyme system composition. | filamentous fungi are considered as the most efficient producers expressing lignocellulose-degrading enzymes. penicillium oxalicum strains possess extraordinary fungal lignocellulolytic enzyme systems and can efficiently utilize plant biomass. in recent years, the regulatory aspects of production of hydrolytic enzymes by p. oxalicum have been well established. this review aims to discuss the recent developments for the production of lignocellulolytic enzymes by p. oxalicum. the main cellulolytic ... | 2017 | 28684177 |
| deletion of ligd significantly improves gene targeting frequency in the lignocellulolytic filamentous fungus penicillium oxalicum. | to improve the gene targeting frequency (gtf) in the lignocellulolytic filamentous fungus penicillium oxalicum hp7-1, the non-homologous end-joining (nhej) gene ligd was deleted. the obtained poligd deletion mutant δpoligd showed no apparent defect in cellulase production, growth rate, and sensitivity towards osmotic stress and mutagen ethyl methanesulphonate (ems), while increased sensitivity to high concentrations of methyl methanesulfonate (mms). deletion of poligd gene resulted in significan ... | 2017 | 28606356 |
| combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in penicillium oxalicum. | lignocellulolytic enzymes are the main enzymes to saccharify lignocellulose from renewable plant biomass in the bio-based economy. the production of these enzymes is transcriptionally regulated by multiple transcription factors. we previously engineered penicillium oxalicum for improved cellulase production via manipulation of three genes in the cellulase expression regulatory network. however, the potential of combinational engineering of multiple regulators and their targets at protein abundan ... | 2017 | 28428823 |