| an entomoepidemiological investigation of chagas disease in the state of ceará, northeast region of brazil. | the seroprevalence of chagas disease in humans and the presence of triatomines were investigated in a rural locality in the state of ceará, brazil, an historically endemic region. approximately 80% of the surveyed residents agreed to undergo serological tests. intradomestic and peridomestic environments were searched for triatomines in both the dry and rainy seasons. the prevalence rate of chagas disease was 1.2% and the majority of individuals confirmed with the disease over 50 years of age. a ... | 2014 | 24896053 |
| insights into a hotspot in the brasiliensis subcomplex (hemiptera, triatominae) by analysis of d2 domain of the nuclear gene 28s. | the brasiliensis subcomplex is a monophyletic group formed by the species triatoma brasiliensis brasiliensis, t. b. macromelasoma, t. juazeirensis, t. melanica, and t. sherlocki. however, using cytogenetic data and experimental hybrid crosses, t. lenti and t. petrochiae were also grouped into this subcomplex. this study aims to analyze the properties of hotspot in the d2 domain of the nuclear gene 28s in all species of the brasiliensis subcomplex as well as t. lenti and t. petrochiae. these spec ... | 2016 | 27050995 |
| distributional potential of the triatoma brasiliensis species complex at present and under scenarios of future climate conditions. | the triatoma brasiliensis complex is a monophyletic group, comprising three species, one of which includes two subspecific taxa, distributed across 12 brazilian states, in the caatinga and cerrado biomes. members of the complex are diverse in terms of epidemiological importance, morphology, biology, ecology, and genetics. triatoma b. brasiliensis is the most disease-relevant member of the complex in terms of epidemiology, extensive distribution, broad feeding preferences, broad ecological distri ... | 2014 | 24886587 |
| a multi-species bait for chagas disease vectors. | triatomine bugs are the insect vectors of trypanosoma cruzi, the etiological agent of chagas disease. these insects are known to aggregate inside shelters during daylight hours and it has been demonstrated that within shelters, the aggregation is induced by volatiles emitted from bug feces. these signals promote inter-species aggregation among most species studied, but the chemical composition is unknown. | 2014 | 24587457 |
| homogeneity of trypanosoma cruzi i, ii, and iii populations and the overlap of wild and domestic transmission cycles by triatoma brasiliensis in northeastern brazil. | the genetic variability of 24 trypanosoma cruzi isolates from humans (11) and triatomines (13) in northeastern brazil was analyzed by random amplified polymorphic dna (rapd) and compared with taxonomic groups, host, and geographical origin of the parasite. tci (12.5%), tcii (45.8%), and tciii (41.6%) showed a similarity coefficient (sc) of 0.74 using the mean of three primers and 0.80, 0.75, and 0.66 for λgt11-f, m13-40f, and l15996 primers, respectively. the samples were clustered according to ... | 2013 | 23371496 |
| spraying food sources with pyrethroid to control peridomestic triatomines. | we attempted to supplement traditional insecticide spraying by treating peridomiciliar food sources with a powder formulation. | 2013 | 24270255 |
| (4s,5s)-2,2,4-triethyl-5-methyl-1,3-dioxolane: a new volatile released by a triatomine bug. | adults of the triatomine bug triatoma brasiliensis release 2,2,4-triethyl-5-methyl-1,3-dioxolane (1) as a mixture of the (4s,5s)- and (4r,5r)-enantiomers in a ratio of 4:1. among the volatile acetals identified from insects so far, this is the first example resulting from an intermolecular condensation of a carbonyl moiety and a diol substructure. | 2010 | 21087036 |
| sequence characterization and expression patterns of defensin and lysozyme encoding genes from the gut of the reduviid bug triatoma brasiliensis. | the cdnas encoding an intestinal defensin (def1) and lysozyme (lys1) of the reduviid bug triatoma brasiliensis have been amplified by pcr using specific oligonucleotide primers and 5'- and 3'-race, cloned and sequenced. the 576 bp clone has an open reading frame of 282 bp and encodes a pre-prodefensin with 94 amino acid residues, containing a putative signal and activation peptide cleavage site at ser19 and arg51, respectively. the genomic dna contains a second defensin gene with similar charact ... | 2006 | 16835020 |
| [biological potential of triatoma brasiliensis]. | biological and physiological parameters of triatoma brasiliensis, triatoma infestans, and triatoma pseudomaculata were studied and compared. t. brasiliensis, reared on mice, showed a faster evolutionary cycle from first stage to adult and higher fecundity, when compared to the other species. t. infestans showed the fastest blood intake in both hosts tested, followed by t. brasiliensis and t. pseudomaculata. clotting tests using salivary gland extracts of t. brasiliensis presented intermediary va ... | 2000 | 11119327 |
| [biology of triatoma braziliensis. ii. observations on its autogeny]. | | 1975 | 766149 |
| modeling disease vector occurrence when detection is imperfect ii: drivers of site-occupancy by synanthropic triatoma brasiliensis in the brazilian northeast. | understanding the drivers of habitat selection by insect disease vectors is instrumental to the design and operation of rational control-surveillance systems. one pervasive yet often overlooked drawback of vector studies is that detection failures result in some sites being misclassified as uninfested; naïve infestation indices are therefore biased, and this can confound our view of vector habitat preferences. here, we present an initial attempt at applying methods that explicitly account for im ... | 2014 | 24811125 |
| mitochondrial pcr-rflp assay to distinguish triatoma brasiliensis macromelasoma from triatoma brasiliensis brasiliensis subspecies (hemiptera: reduviidae). | triatoma brasiliensis sensu lato (s.l.), the main vector of chagas disease in northeastern brazil, is a species complex comprising four species, one with two subspecies (t. brasiliensis brasiliensis, t. brasiliensis macromelasoma, t. juazeirensis, t. sherlocki, and t. melanica), and each taxon displaying distinct ecological requirements. in order to evaluate the genetic relationships among nine t. brasiliensis s.l. populations from northeastern brazil, we analyzed their mitochondrial cytochrome ... | 2013 | 24454408 |
| development of conventional and real-time multiplex pcr-based assays for estimation of natural infection rates and trypanosoma cruzi load in triatomine vectors. | chagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. the diversity of vector species and their habitats are among the factors that hinder the control of the disease. control programs periodically monitor the prevalence of t. cruzi infection in insect bugs through microscopical observation of diluted feces. however, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all ... | 2017 | 28851417 |
| high triatoma brasiliensis densities and trypanosoma cruzi prevalence in domestic and peridomestic habitats in the state of rio grande do norte, brazil: the source for chagas disease outbreaks? | abstracta total of 2,431 triatoma brasiliensis were collected from 39 populations of paraíba (pb) and rio grande do norte (rn) states, brazil. in pb, trypanosoma cruzi infection was not detected in either peridomestic or domestic vector populations. in contrast, in rn, t. brasiliensis was detected with high parasite prevalence in these ecotopes (30.7-40.0%). moreover, peridomicile insect population densities were more than double the average densities of all other settings evaluated (19.17 versu ... | 2017 | 28719275 |
| evaluation of rbcl and crclôéâ as markers of triatoma brasiliensis (hemiptera: reduviidae) nymphs: persistence and influence of rb and cr on triatomine biology. | in order to mark triatoma brasiliensis, the vector of chagas disease in brazil, two chemical compounds, rubidium chloride (rbcl) and chromium chloride (crclôéâ), were tested. first, 199 n2-n5 nymphs were fed on blood with 0.025m rbcl. rb marker positivity ranged from 2.5% (n3)-70% (n2), with a maximum persistence of 98 days. second, 265 n2-n5 nymphs were fed on blood containing 0.0015m crclôéâ. cr marker positivity ranged up to 93% (n5), with a maximum persistence of 119 days. finally, we blood ... | 2011 | 21739023 |
| Combining geospatial analysis and exploratory study of triatomine ecology to evaluate the risk of Chagas disease in a rural locality. | The use of geo-spatial analysis to anticipate transmission risk for Chagas disease was tested in a rural area of northeast Brazil in an approach that combined geo-referencing and exploratory study of triatomine infestation, including related elements such as the environment and hosts. A total of 617 triatomine specimens, mainly Triatoma brasiliensis, were captured, exhibiting an overall T. cruzi positivity of 44.4%. Layer analysis indicated that the greatest transmission risk to man was associat ... | 2012 | 22015423 |
| [resistence of triatoma brasiliensis to fasting]. | | 2015 | 4596930 |
| a common caatinga cactus, pilosocereus gounellei, is an important ecotope of wild triatoma brasiliensis populations in the jaguaribe valley of northeastern brazil. | triatoma brasiliensis is the most important vector of chagas disease in the caatinga eco-region of northeastern brazil. wild t. brasiliensis populations have been reported only from rocky outcrops. however, this species frequently infests/re-infests houses in rock-free sedimentary lowlands. we therefore hypothesized that it should also occupy other natural ecotopes. we show that a common caatinga cactus, pilosocereus gounellei, locally known as xiquexique, often harbors t. brasiliensis breeding ... | 2014 | 24710611 |
| multiple mitochondrial genes of some sylvatic brazilian triatoma: non-monophyly of the t. brasiliensis subcomplex and the need for a generic revision in the triatomini. | multiple fragments of mitochondrial dna genes (cytochrome b, cytochrome oxidase i, and 16s rdna) were used to evaluate the phylogenetic relationships among triatoma melanocephala, triatoma tibiamaculata, triatoma vitticeps, and other members of triatoma brasiliensis subcomplex under a bayesian framework and maximum parsimony criterion. with the addition of new sequences of t. tibiamaculata and t. vitticeps, triatoma juazeirensis, triatoma melanica and the newly sequenced t. melanocephala, the th ... | 2014 | 24508245 |
| revalidation and redescription of triatoma brasiliensis macromelasoma galvão, 1956 and an identification key for the triatoma brasiliensis complex (hemiptera: reduviidae: triatominae). | triatoma brasiliensis macromelasoma is revalidated based on the results of previous multidisciplinary studies on the triatoma brasiliensis complex, consisting of crossing experiments and morphological, biological, ecological and molecular analyses. these taxonomic tools showed the closest relationship between t. b. macromelasoma and triatoma brasiliensis brasiliensis. t. b. macromelasoma is redescribed based on specimens collected in the type locality and specimens from a f1 colony. the complex ... | 2013 | 24037202 |
| cross-mating experiments detect reproductive compatibility between triatoma sherlocki and other members of the triatoma brasiliensis species complex. | phylogenetic approaches based on mitochondrial dna variation (fragments of cyt b and 16s ribosomal rna) have revealed triatoma sherlocki as the most recent species addition to the triatoma brasiliensis species complex; a monophyletic group which includes t. brasiliensis, triatoma melanica, and triatoma juazeirensis. t. sherlocki is the most differentiated among all species of this complex: it is unable to fly, possesses longer legs than the other members, and has reddish tonality in some parts o ... | 2013 | 23850508 |
| ecotope effect in triatoma brasiliensis (hemiptera: reduviidae) suggests phenotypic plasticity rather than adaptation. | triatoma brasiliensis (hemiptera: reduviidae) is an important vector of chagas' disease in both sylvatic and peridomestic ecotopes. discriminating between these populations of triatominae has been proposed as a means of investigating re-infestation rates of human dwellings. geometric morphometrics have been widely applied in the study of triatominae polymorphisms at species and population levels. this study characterizes morphometric differences between sylvatic and peridomestic populations, as ... | 2013 | 22985051 |
| [microclimatic properties of the triatoma brasiliensis habitat]. | vector-borne transmission of chagas disease in northeast brazil is basically by triatoma brasiliensis. it is thus crucial to determine this species' microclimatic preferences as limiting factors for its distribution and ability to infest domestic environments. we analyze the microclimatic properties of the shelters in which these insects are found in wild, domestic, and peridomiciliary environments in the state of ceará, at brazil. we measure temperature and relative humidity (rh) every 15 minut ... | 2000 | 11119321 |
| morphology of the spermathecae of twelve species of triatominae (hemiptera, reduviidae) vectors of chagas disease. | trypanosoma cruzi, the etiological agent of chagas disease, is transmitted by triatomines that have been described in a large number of studies. most of those studies are related to external morphology and taxonomy, but some biochemical, genetic and physiological studies have also been published. there are a few publications in the literature about the internal organs of triatominae, for instance the spermathecae, which are responsible for storing and maintaining the viability of the spermatozoi ... | 2017 | 28859957 |
| sperm dimorphism in the triatoma brasiliensis species complex and its applications. | morphological and structural features of the sperm of the triatoma brasiliensis neiva, 1911 (hemiptera: reduviidae) species complex were examined in this first study investigating the sperm of heteroptera and the genus triatoma. males were dissected and their seminal vesicles removed. for measurement, seminal vesicles were squashed on glass slides, spread, fixed and observed under a photomicroscope. the images were analysed and measures of sperm were made. data were analysed using one-way analys ... | 2017 | 28117911 |
| thermal preferences and limits of triatoma brasiliensis in its natural environment--field observations while host searching. | the goal of this work was to explore the thermal relationship between foraging triatoma brasiliensis and its natural habitat during the hottest season in the state of ceará, brazil. the thermal profiles were determined using infrared analysis. although the daily temperature of rock surfaces varied in a wide range, t. brasiliensis selected to walk through areas with temperatures between 31.7-40.5ºc. the temperature of t. brasiliensis body surface ranged from 32.8-34.4ºc, being higher in legs than ... | 2015 | 26517659 |
| cytotaxonomy of the brasiliensis subcomplex and the triatoma brasiliensis complex (hemiptera: reduviidae: triatominae). | we analyzed the classical cytotaxonomy of the brasiliensis subcomplex (triatoma brasiliensis neiva, t. juazeirensis costa & felix, t. melanica costa, argolo & felix, t. melanocephala neiva & pinto, t. petrochiae pinto & barreto, t. lenti sherlock & serafim, t. sherlocki papa, jurberg, carcavallo, cerqueira & barata, t. tibiamaculata pinto and t. vitticeps stal) and the t. brasiliensis complex (t. b. brasiliensis, t. b. macromelasoma neiva & lent, t. juazeirensis, t. melanica and t. sherlocki). t ... | 2014 | 25081800 |
| serine carboxypeptidases of triatoma brasiliensis (hemiptera, reduviidae): sequence characterization, expression pattern and activity localization. | using specific oligonucleotides, 5'- and 3'-race and sequencing, two cdnas encoding serine carboxypeptidases (tbscp-1 and tbscp-2) from the midgut of the blood sucking heteropteran triatoma brasiliensis were identified. both cdnas with an open reading frame of 1389bp, encode serine carboxypeptidase precursors of 463 amino acid residues, which possess a signal peptide cleavage site after ala19. analysis of tbscp-1 and tbscp-2 genomic dna showed an absence of introns in both sequences and the pres ... | 2014 | 24548612 |
| phenotypic variability confirmed by nuclear ribosomal dna suggests a possible natural hybrid zone of triatoma brasiliensis species complex. | triatoma brasiliensis macromelasoma occurs in pernambuco state, brazil, which is situated between the distribution areas of triatoma brasiliensis brasiliensis (north) and triatoma juazeirensis (south). t. b. macromelasoma displays greater variations in its chromatic phenotype than either t. b. brasiliensis or t. juazeirensis, and patterns reminiscent of one or the other. experimental crosses from each of these members of the t. brasiliensis species complex generated fertile offspring suggesting ... | 2016 | 26520796 |
| ribosomal dna its-1 intergenic spacer polymorphism in triatomines (triatominae, heteroptera). | the length polymorphism of ribosomal dna its-1 intergenic spacer was analyzed in eight species of triatomines belonging to triatoma, rhodnius, and panstrongylus genera. the analyzed species were rhodnius domesticus, r. neivai, r. robustus, triatoma brasiliensis, t. infestans, t. vitticeps, panstrongylus megistus, and p. herreri. these insects are vectors of chagas' disease, one of the most prominent public health problems among south american countries. this work allowed the differentiation betw ... | 2005 | 16187161 |
| entomological survey in the state of piauí, northeastern brazil, reveals intradomiciliary colonization of triatoma brasiliensis macromelasoma. | this survey aimed to assess the presence of triatomine vectors of chagas disease within the rural communities of são joão do piauí, northeast brazil. intradomiciliary and peridomiciliary collection strategies were implemented wherein 279 specimens of triatoma brasiliensis macromelasoma, both nymph and adult were found in 15 (50%) of the studied homes. of the intradomiciliary insects, 73 (67.6%) were identified as nymph instars (1st instar [n1]= 6, n2= 14, n3= 28, n4= 7 and n5= 18). in the studie ... | 2017 | 28591255 |
| genes encoding defensins of important chagas disease vectors used for phylogenetic studies. | insects possess both cellular and humoral immune responses. the latter makes them capable to recognize and control invading pathogens after synthesis of a variety of small proteins, also known as antimicrobial peptides. defensins, cysteine-rich cationic peptides with major activity against gram-positive bacteria, are one ubiquitous class of antimicrobial peptides, widely distributed in different animal and plant taxa. regarding triatomines in each of the so far analyzed species, various defensin ... | 2015 | 26337269 |
| chagas disease and housing improvement in northeastern brazil: a cross-sectional survey. | chagas disease was investigated in two new settlements of landless rural workers inhabiting prefabricated, triatomine-proof houses and in four neighboring older communities where mud huts were still well suitable for vectors. through a cross-sectional survey and entomological assessment, we surveyed 148 houses/families in the two new settlements and in 47 houses/families in the four older localities. we determined seroprevalence of chagas disease through ifi and elisa (eluates) assays and search ... | 2015 | 25673077 |
| de novo transcriptome assembly for a non-model species, the blood-sucking bug triatoma brasiliensis, a vector of chagas disease. | high throughput sequencing (hts) provides new research opportunities for work on non-model organisms, such as differential expression studies between populations exposed to different environmental conditions. however, such transcriptomic studies first require the production of a reference assembly. the choice of sampling procedure, sequencing strategy and assembly workflow is crucial. to develop a reliable reference transcriptome for triatoma brasiliensis, the major chagas disease vector in nort ... | 2015 | 25233990 |