| effects of amidination and chemical cross-linking on human factor viii (antihemophilic factor). | the bifunctional reagent dimethyl suberimidate, reacting with primary amino groups of proteins, was used to cross-link highly purified human factor viii. reaction products were reduced with beta-mercaptoethanol or treated with rhizopus arrhizus triglyceride lipase. the proportions of the dissociated subunits and their oligomers were calculated from the relative staining intensities of individual bands following polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate. low concent ... | 1977 | 410451 |
| phycomycotic gangrenous cellulitis. a report of two cases and a review of the literature. | progressive gangrenous cellulitis due to rhizopus arrhizus following colostomy destroyed the entire abdominal wall of a young woman and caused her death. a similar infection in an 11-year-old kidney transplant recipient was diagnosed more promptly and treated successfully with extensive debridement and amphotericin b. nine similar cases found in the literature were reviewed. all 11 patients appeared to have had prior tissue injury at the original site of infection, and seven had diabetes mellitu ... | 1976 | 131532 |
| simplified, totally enzymatic method for determination of serum triglycerides with a centrifugal analyzer. | we describe a totally enzymatic method for determination of serum triglycerides (triacylglycerols) specifically adaptable to the centrifichem system. the method involves lipolysis with lipase from rhizopus arrhizus alone and quantitation of the resulting glycerol with glycerol dehydrogenase in a kinetic, fixed-time mode. hydrolysis by the lipase is complete, for concentrations up to at least 5.0 g/liter, in 10 min at room temperature. the unfavorable equilibrium for the oxidation of glycerol is ... | 1976 | 7367 |
| proteolysis of von willebrand factor by lipase preparations from rhizopus arrhizus. | | 1987 | 3498236 |
| biosynthesis of paf-acether factor-acether by human skin fibroblasts in vitro. | the synthesis and release of paf-acether by fibroblasts from normal human skin was investigated in vitro. when fibroblasts in suspension (1 x 10(6) cells) were stimulated with 2 microm ca1+ ionophore a23187 (io), they synthesized a material that aggregated aspirin-treated washed rabbit platelets and was identified as paf because 1) the platelet aggregation it induced was inhibited by bn 52021, an antagonist of paf putative receptors; 2) the factor was inactivated by phospholipase a2 but was inse ... | 1988 | 3397536 |
| [comparison of fungal lipase and pancreatic lipase in exocrine pancreatic insufficiency in man. study of their in vitro properties and intraduodenal bioavailability]. | assuming that acidic degradation of lipase was the major cause of failure for the correction of steatorrhea by pancreatic extracts, we compared the in vitro and in vivo activities of a fungal lipase (fl) (rhizopus arrhizus) with classical porcine pancreatic extract (eurobiol). the choice of fl was determined by its two optimum ph (3.5 and 7.4). five factors known to modify lipase activity were tested: ph, biliary acids colipase, trypsin and albumin. bioavailability was measured by using a double ... | 1988 | 3220231 |
| mucormycosis in diabetic ketoacidosis. role of unbound iron binding capacity of transferrin. | increased susceptibility to rhizopus oryzae in diabetes was investigated using mice in which diabetic ketoacidosis was experimentally induced. all of the diabetic mice which had developed ketoacidosis died within four days after inoculation of r. oryzae and their serum uibcs (unbound iron binding capacity) were significantly lower than those of the normal control mice. this study suggested that the decreased serum uibc produced by diabetic ketoacidosis enhances the growth of r. oryzae in vivo. | 1986 | 3099540 |
| pyrenemethyl laurate, a new fluorescent substrate for continuous kinetic determination of lipase activity. | a fluorescent acyl derivative of pyrenemethanol, pyrenemethyl laurate, was synthesized and used for the determination of several lipases by a continuous kinetic assay. the influence of the physical parameters of the substrate (pyrenemethyl laurate) and its hydrolysis product (pyrenemethanol), on the fluorescence emission was studied. the hydrolysis of pyrenemethyl laurate could be monitored directly in a spectrofluorometer because of the very high monomeric emission of pyrenemethanol at about 37 ... | 1989 | 2804074 |
| a study on the inhibition of aflatoxin b1 induced hepatocarcinogenesis by the rhizopus delemar. | the effect of rhizopus delemar on the carcinogenicity in rats of aflatoxin b1 was studied. the aflatoxin b1 was administered in free drinks to each male wistar rat at 126 micrograms per week such that a total dose of 3.40 mg was given over a period of 27 weeks. the culture abstract of rhizopus delemar was added simultaneously to a group of these rats by mixing the aflatoxin b1 solution. animals were killed separately during 18th, 30th, 38th and 52nd week. liver cell altered foci and neoplasms we ... | 1989 | 2518699 |
| purification of plasmalogens using rhizopus delemar lipase and naja naja naja phospholipase a2. | bovine heart chogpl (choline glycerophospholipid) and bovine brain etngpl (ethanolamine glycerophospholipid) contain diacyl, alkenylacyl and alkylacyl analogs. purification of plasmalogens was achieved using r. delemar lipase and n. naja naja phospholipase a2 digestion. the r. delemar lipase hydrolyzes the acyl bond at the 1-position of 1,2-diacyl glycerophospholipids. the n. naja naja phospholipase a2 has greater activity with diacyl and alkylacyl than with alkenylacyl glycerophospholipids. the ... | 1990 | 2366631 |
| acid triacyglycerol lipase inhibitor in chicken plasma: purification and properties. | 1. acid triacylglycerol lipase inhibitor was highly purified from chicken plasma by ammonium sulfate fractionation (0.6-1 saturation) followed by successive chromatographies on hydroxyapatite, blue-cellulofine, phenyl-sepharose and cellulofine gcl-2000 columns, and isoelectric focusing. 2. the lipase inhibitor showed its inhibitory action on triacylglycerol lipases in chicken erythrocytes ghosts and in chicken liver lysosome, but did not on pancreatic lipase, rhizopus arrhizus lipase, or wheat g ... | 1990 | 2279620 |
| invasive polymycotic pneumonia in an uncontrolled diabetic. | we describe the clinical course of a patient with invasive polymycotic pneumonia due to rhizopus arrhizus and candida albicans. both organisms were recovered from antemortem sputum cultures, and their clinical significance was confirmed by histologic examination of the lungs at autopsy. circumstances leading to polymycotic infection are discussed, with special attention given to polymycotic infections involving zygomycetes. | 1991 | 2021322 |
| microbial transformation of zearalenone to a zearalenone sulfate. | the conversion of zearalenone by various microorganisms was studied. a new polar metabolite was formed in addition to alpha- and beta-zearalenols. the structure of the new metabolite was determined as zearalenone-4-o-sulfate conjugate on the basis of enzymatic and acid hydrolysis, followed by mass spectrometry, nuclear magnetic resonance, and infrared spectroscopic analysis. the results obtained demonstrate that rhizopus arrhizus catalyzes sulfation of zearalenone at the c-4 hydroxyl group. | 1991 | 1826596 |
| cloning, expression and characterization of a cdna encoding a lipase from rhizopus delemar. | a lambda gt11 cdna library was constructed in escherichia coli using poly(a)-selected mrna from the fungus, rhizopus (rp.) delemar. lipase-producing members of the library were identified by means of a phenotypic score wherein the release of fatty acids by lipase causes a characteristic color change in the growth medium. one such isolate contained a 1287-bp insert (lip cdna) which hybridizes to 1.25- to 1.35-kb mrna species from rp. delemar. the lipase produced in e. coli containing the lip cdna ... | 1991 | 1756969 |
| the glucoamylase cdna from aspergillus oryzae: its cloning, nucleotide sequence, and expression in saccharomyces cerevisiae. | a cdna for aspergillus oryzae glucoamylase was cloned, using oligodeoxyribonucleotide probes derived from amino sequences of peptide fragments of the enzyme. the glucoamylase cdna, when introduced into saccharomyces cerevisiae, directed the secretion of active glucoamylase into the culture medium. the complete nucleotide sequence of the cdna contained an open reading frame encoding 612 amino acid residues. comparative studies with other fungal glucoamylases showed homologies of 67% with a. niger ... | 1991 | 1368680 |
| activities of candida rugosa lipase and other esterolytic enzymes coated on glass beads and suspended in substrate and water vapor: enzymes in thin liquid films. | candida rugosa lipase was enzymatically active when coated on glass beads and exposed to mixtures of substrate and water vapor over a range of relative humidities up to 100%. evidence was obtained for operation of the enzyme in a thin liquid film of concentrated buffer on the surface of the glass beads. formation of the thin film was associated with hygroscopicity of the buffer used to suspend the enzyme in preparation of the enzyme-coated beads. at some buffer concentrations estimated to be on ... | 1991 | 1367174 |
| an improved method for the colorimetric assay of lipase activity using an optically clear medium. | lipase activity can be spectrophometrically measured in an optically clear medium using long chain fatty thioesters of 1-mercapto-2,3-propanediol or 2-mercaptoethanol as substrates. with hexamethylphosphoric triamide solutions of these thiosubstrates, the michaëlis-menten constants of lipase from rhizopus arrhizus were determined. the effects of calcium chloride and of bovine serum albumin on the enzyme activity were established. | 1987 | 3626781 |
| an enzymatic colorimetric assay of calcium-dependent phospholipases a. | in this paper we describe a new method for the assay of calcium-dependent phospholipases a. in this method released fatty acids are quantitated by an enzymatic colorimetric reagent kit which is commercially available. we have tested the applicability of this assay with enzymes from porcine pancreas (phospholipase a2), snake venom (phospholipase a2), and rhizopus arrhizus (a lipase with phospholipase a1-like activity) as well as with a phospholipase a2 activity of bovine seminal vesicle fluid. we ... | 1987 | 3674375 |
| a simple enzymatic fluorimetric method for the determination of triglycerides in 10 microliters of serum. | an assay for the determination of triglyceride (acyl glycerol) in microliter volumes of serum or plasma is described. the method is based on enzymatic hydrolysis of triglyceride by lipase from rhizopus arrhizus followed by enzymatic fluorimetric assay of the glycerol so released. under the conditions of the assay the enzymatic hydrolysis is complete for concentrations up to 5 mmol/l in less than 15 min at room temperature. the determination of free glycerol and glycerol released is based on the ... | 1986 | 3698322 |
| inhibition of lipases by proteins: a binding study using dicaprin monolayers. | we previously reported that the inhibition of pancreatic and rhizopus delemar lipases by proteins is due to the protein associated with lipid and is not caused by direct protein-enzyme interaction in the aqueous phase [gargouri, y., piéroni, g., rivière, c., sugihara, a., sarda, l., & verger, r. (1985) j. biol. chem. 260, 2268-2273]. in this study, using radiolabeled lipases, serum albumin, and beta-lactoglobulin a, we investigated their respective binding with respect to lipolysis of dicaprin m ... | 1986 | 3707907 |
| rapid accumulation of diacyl lipid in rat liver microsomes by selective acylation of 2-acyl-sn-glycero-3-phosphorylserine. | 2-acyl-sn-glycero-3-phosphoryl[u-14 c]serine (2-acylgps) was prepared by digesting 1,2-dioleoyl-sn-glycero-3-phosphoryl[u-14c]serine, mixed with pig brain phosphatidylserine (ps), with rhizopus arrhizus lipase. the labeled lysolipid was shown to be actively acylated by rat liver microsomes in the presence of acyl-coa thioesters, with the formation of diacylps. in experiments with unlabeled lysolipid and [14c]acyl-coa thioesters, digestion of the products with pancreatic phospholipase a2 showed t ... | 1986 | 3707975 |
| fluorometric procedures for measuring triglyceride concentrations in small amounts of tissue and plasma. | it has been previously shown that triglycerides can be specifically hydrolyzed by lipase from rhizopus arrhizus in the presence of hog liver esterase and sodium dodecyl sulfate. the glycerol produced can then be measured by sequential reactions with glycerokinase, pyruvate kinase, and lactate dehydrogenase: glycerol and atp are converted to glycerol-3-phosphate and adp by glycerokinase; the adp reacts with phosphoenolpyruvate and pyruvate kinase to yield pyruvate; the pyruvate is converted to la ... | 1986 | 3723017 |
| modification of serum, pancreatic, and microbial lipase activities by phorbol diesters. | the influence of phorbol diesters on the in vitro hydrolysis of diacylglycerols was examined using enzymes from rat serum, porcine pancreas, and rhizopus delemar. two main phenomena were observed: 12-o-tetradecanoylphorbol-13-acetate (tpa), when added to the enzyme assay system, stimulated 2- to 3-fold the hydrolysis of [9,10-3h]dioleoylglycerol by serum lipase. the hydrolysis of dioleoylglycerol by either pancreatic or r. delemar lipase was, on the other hand, inhibited by tpa. a 50% inhibition ... | 1987 | 3791200 |
| lipids of rhizopus arrhizus fischer. | the lipids of rhizopus arrhizus fischer mycelia and sporangiospores were extracted, isolated, and separated by thin-layer, liquid, and gas chromatography. structural confirmations of the compounds were made by a gas chromatographmass spectrometer combination. the n-heptane fraction contained squalene (1%) as a major hydrocarbon constituent. other major lipid classes detected were free fatty acids, naturally occurring methyl esters of fatty acids, triglycerides, sterols, and polar lipids. the pol ... | 1970 | 5474875 |
| [some morphological and physiological properties of rhizopus delemar]. | | 1970 | 5497282 |
| pharmacological and toxicological studies on a starch diastatic enzyme "new sumyzyme" produced by rhizopus delemar var. multiplici-sporus. | | 1970 | 5535754 |
| [lipase of rhizopus arrhizus. isolation and properties of the pure enzyme]. | | 1968 | 5716418 |
| studies on carbohydrate-metabolizing enzymes. 11. the hydrolysis of lichenin by enzyme preparations from malted barley and rhizopus arrhizus. | | 1964 | 5833367 |
| role of proline and glutamic acid metabolism in spore germination of rhizopus arrhizus. | | 1966 | 5901876 |
| further studies on germination of sporangiospores of rhizopus arrhizus. | | 1966 | 5912591 |
| [lipase of rhizopus aerhizus. obtaining, purification and properties of the lipase of rhizopus arrhizus var. delemar]. | | 1966 | 5958554 |
| a kinetic method for the determination of number-average molecular weight of linear high polymer by using an exo-enzyme. number-average molecular weights of various fractions of amylose determined by using gluc-amylase of rhizopus delemar. | | 1966 | 5970083 |
| antifungal activity of isothiocyanates and related compounds. i. naturally occurring isothiocyanates and their analogues. | data are presented concerning the antifungal activity of 11 natural isothiocyanates and 27 synthetized analogues in aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as in 13 additional saprophytic and parasitic fungi. a remarkable antifungal activity was observed in some analogues of benzylisothiocyanate and beta-phenylethylisothiocyanate. the latter-mentioned compounds have not been described previously. in the group of benzylisothiocyanates, a correlation, which was inver ... | 1967 | 6049294 |
| antifungal activity of isothiocyanates and related compounds. ii. mononuclear aromatic isothiocyanates. | antifungal activity on aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as on additional saprophytic and parasitic fungi, was determined in 57 substituted derivatives of phenylisothiocyanate. most of the investigated compounds displayed rather equal activity against the three mentioned fungi, in contradistinction to the analogues of natural benzyl- and beta-phenylethylisothiocyanate with their characteristic low activity against r. oryzae. differences occurred in the type o ... | 1967 | 6049295 |
| [on rhizopus arrhizus lipase. i. positional specificity]. | | 1967 | 6055266 |
| purification of a lipase from rhizopus arrhizus. identification of two active forms of the enzyme. | | 1967 | 6078132 |
| utilization of diacylglycerol in phospholipid bilayers by pig brain diacylglycerol kinase and rhizopus arrhizus lipase. | diacylglycerol kinase purified from pig brain cytosol could use sonication-dispersed diacylglycerol in the presence of its activator, phosphatidylcholine vesicles. however, the kinase failed to significantly use diacylglycerol cosonicated with phosphatidylcholine. similarly, the kinase could not use diacylglycerol generated in microsomes by the back reaction of diacylglycerol choline phosphotransferase, though phospholipase c treatment of microsomes yielded effective substrate for the kinase. in ... | 1984 | 6088535 |
| [further examinations of the influence of somatostatin on triglyceride absorption (author's transl)]. | 7 healthy subjects received an oral fat load containing 100 g neutral fat on two test days. the test meal was followed by an infusion of somatostatin (500 micrograms/h) over three hours. on the first test day 150,000 e of an active fungal lipase (extracted from rhizopus arrhizus) were given with the fat meal. on the second day placebo capsules were administered instead of lipase. no postprandial increase of serum triglyceride levels could be found in both examinations. these results suggest, tha ... | 1980 | 6104384 |
| antigenic characterization of some potentially pathogenic mucoraceous fungi. | the antigenic profiles of 10 mucoraceous fungi--absidia corymbifera, mortierella wolfii, mucor miehei, m. pusillus, m. racemosus, rhizopus arrhizus, r. microsporus, r. oryzae, r. rhizopodiformis, r. stolonifer,--candida albicans and aspergillus fumigatus were compared by crossed immunoelectrophoresis (xie). antigen-rich material was obtained from homogenized hyphae (or yeasts in the case of c. albicans), and antisera by multiple subcutaneous innoculation of rabbits with macerated but viable hyph ... | 1982 | 6182620 |
| hydroxamate siderophore production by opportunistic and systemic fungal pathogens. | it has been suggested that siderophores may function as virulence factors. there have been few studies on production of siderophores by opportunistic and pathogenic fungi. we examined siderophore production by absidia corymbifera, aspergillus niger, rhizopus arrhizus, rhizopus oryzae, blastomyces dermatitidis, histoplasma capsulatum, sporothrix schenickii, candida albicans, and trichophyton mentagrophytes. fungi were cultured at 37 and 27 degrees c in a chemically defined low-iron media (0.2 mic ... | 1983 | 6221998 |
| selective effects of two systemic fungicides on soil fungi. | bas 317 00f was not toxic to the total count of fungi after 2 days but was regularly significantly toxic at the three doses after 5, 20 and 40 days and toxic at the low and the high doses after 80 days. in the agar medium, it was toxic to the counts of total fungi. aspergillus, a. terreus, rhizopus oryzae and mucor racemosus at the high dose. only the mycelial growth of trichoderma viride which was significantly inhibited by the three doses when this fungicide was added to the liquid medium. pol ... | 1982 | 6290890 |
| damage to aspergillus fumigatus and rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro. | our previous studies established that human neutrophils could damage and probably kill hyphae of aspergillus fumigatus and rhizopus oryzae in vitro, primarily by oxygen-dependent mechanisms active at the cell surface. these studies were extended, again quantitating hyphal damage by reduction in uptake of (14)c-labeled uracil or glutamine. neither a. fumigatus nor r. oryzae hyphae were damaged by neutrophils from patients with chronic granulomatous disease, confirming the importance of oxidative ... | 1982 | 6292103 |
| in vivo bronchoalveolar macrophage defense against rhizopus oryzae and aspergillus fumigatus. | the ability of bronchoalveolar macrophages from normal, diabetic, and cortisone-treated mice to inhibit spore germination and kill fungal spores in vivo was investigated. the data indicated that the normal host controls different fungal infections in the lungs by different mechanisms. prevention of mucormycosis required inhibition of fungal spore germination by alveolar macrophages. in contrast, pulmonary defense against aspergillosis depended on early killing of conidia by alveolar macrophages ... | 1984 | 6387001 |
| immunodiffusion and complement fixation assays with sera from mucormycotic-infected mice. | results using homogenate antigens of rhizomucor pusillus against immunized rabbit sera, mouse ascites fluid and sera from mucormycotic-infected mice, detected by immunodiffusion and complement fixation assays are presented. using immunodiffusion techniques, common antigens and unique antigens from rh. pusillus, rhizopus oryzae and absidia corymbifera were observed. complement fixation titers of greater than 256 and 128 were obtained in immunized rabbit sera and mouse ascites fluid, respectively. ... | 1983 | 6422303 |
| inhibition of pancreatic and microbial lipases by proteins. | we have compared the effect of several proteins, including melittin, beta-lactoglobulin a, serum albumin, ovalbumin and myoglobin, on the hydrolysis of tributyrin and triolein by lipases from various origins. all proteins tested inactivate pancreatic lipase in absence of colipase and bile salt. inhibition is not significantly reversed by colipase in absence of bile salt except in systems containing tributyrin and melittin or triolein and beta-lactoglobulin a. in all other cases, activation of pa ... | 1984 | 6477949 |
| [growth and biotin synthesis of sporobolomyces pararoseus and rhizopus delemar under different substrate ratios]. | biotin synthesis by the yeast sporobolomyces pararoseus and the microscopic fungus rhizopus delemar was studied in batch cultures at different substrate ratios in the medium. the overall (exocellular + endocellular) biotin content in the yeast was 4.5-6.0 micrograms per 1 g of biomass on different media and did not depend on the growth phase. the content of biotin in the fungus was 7.5-9.0 micrograms per 1 g of biomass and depended neither on the growth phase nor on the quantitative ratio betwee ... | 1984 | 6541287 |
| [desthiobiotin transformation to biotin by the fungus rhizopus delemar]. | the object of this work was to study the transformation of desthiobiotin (dtb), a biotin precursor, to biotin by rhizopus delemar which can accumulate rather large quantities of biotin in the medium. both the growing and resting culture transform dtb at a high rate. biotin excretion by the resting culture rises by 100% when 25 micrograms/ml of dtb are added to the medium. the content of biotin within the cells remains unchanged. l-methionine, sodium sulfite and sodium sulfate are most effective ... | 1983 | 6664312 |
| cerebral mucormycosis in diabetic mice after intrasinus challenge. | rhinocerebral involvement is the most common form of mucormycosis in uncontrolled diabetic patients. streptozotocin-induced diabetic and normal mice were challenged by intrasinus inoculation of rhizopus oryzae to determine their susceptibility to infection by this route. ninety percent of diabetic mice and no normal mice died after inoculation with 10(6) spores. intrasinus inoculations caused minimal direct trauma and low levels of brain inocula, but progressive cerebral infections were induced ... | 1984 | 6706405 |
| use of flow cytometry in industrial microbiology for strain improvement programs. | a flow cytometry (fcm) system was chosen to analyze and sort microbiological samples, e.g., bacteria, bacterial spores, yeasts, and fungal spores, without major changes in the commercially available state. the system was further improved by addition of a stepping motor-driven scanning table that accepts standard petri dishes or microtiter plates. the electronics of the sorting system were changed to enable the sorter to deliver only one particle at a time, working in a "handshake" mode with the ... | 1984 | 6714026 |
| antimicrobial effects of esters and amides of 3-(5-nitro-2-furyl)acrylic acid. | the effect of 18 newly synthesized esters and amides of 3-(5-nitro-2-furyl)acrylic acid on bacteria (escherichia coli, staphylococcus aureus), yeasts (saccharomyces cerevisiae, candida albicans), molds (aspergillus niger, penicillium cyclopium, rhizopus oryzae) and algae (chlorella pyrenoidosa, euglena gracilis, scenedesmus obliquus) was investigated. the mic values revealed antimycotic, antialgal and antibacterial activity of the studied derivatives. the antimycotic activity was found to decrea ... | 1984 | 6714854 |
| specific susceptibility to mucormycosis in murine diabetes and bronchoalveolar macrophage defense against rhizopus. | to assess the influence of diabetes mellitus in predisposing to pulmonary mucormycosis, a murine model of streptozotocin-induced diabetes was used. intranasal inoculation of rhizopus oryzae into diabetic mice resulted in mucormycotic infection with histopathology resembling pulmonary mucormycosis observed in humans. there was no mortality nor infection in inoculated normal mice. diabetic mice had fatal infections caused by r. oryzae but significantly reduced mortality following inoculation with ... | 1984 | 6736246 |
| inducibility of cytochrome p-450 and of nadph-cytochrome c reductase in progesterone treated filamentous fungi rhizopus nigricans and rhizopus arrhizus. | | 1981 | 6785529 |
| a mechanism of susceptibility to mucormycosis in diabetic ketoacidosis: transferrin and iron availability. | the defect in host defense that makes the diabetic ketoacidotic (dka) patient susceptible to mucormycosis has not been identified. sera from 10 dka patients and three normal volunteers were tested for their capacity to support the in vitro growth of a common etiologic agent of mucormycosis, rhizopus oryzae. after equilibration with room air none of the normal or dka sera, each of which was now extremely alkaline, supported growth of r. oryzae. when the sera were placed in a co2 atmosphere that p ... | 1982 | 6816646 |
| generation of chemotactic factors by rhizopus oryzae in the presence and absence of serum: relationship to hyphal damage mediated by human neutrophils and effects of hyperglycemia and ketoacidosis. | as our previous studies had shown that human neutrophils could kill rhizopus oryzae hyphae in vitro, interactions of these hyphae with neutrophils and serum were further explored. heated or fresh normal human sera suppressed hyphal metabolic activity as determined by [14c]uracil uptake, but severe ketoacidosis (8 x 10(-3) m beta-hydroxybutyric acid plus 2 x 10(-3) m acetoacetic acid at ph 7.0) negated this effect. hyperglycemia (500 mg/dl) and severe ketoacidosis did not affect damage to hyphae ... | 1982 | 6818145 |
| the comparative virulence of thermotolerant mucorales species in mice. | the comparative virulence of thermotolerant mucorales was determined for cortisone-treated and untreated swiss mice by intravenous administration of spores. the measure of virulence was based on an ld50 value, calculated after the 30-day observation period. of the known etiological agents of mucormycosis, mucor meihei, m. pusillus, rhizopus arrhizus, r. chinensis, r. cohnii, r. microsporus, r. oryzae, r. rhizopodiformis and cunninghamella elegans were able to produce fatal infections in mice; wh ... | 1983 | 6877338 |
| [optimization of the medium for biotin biosynthesis by a culture of rhizopus delemar]. | the effect of nitrogen and carbon sources on the biotin biosynthesis by the fungal culture rhizopus delemar 1428 has been investigated. peptone and soy-bean flour have been shown to stimulate biotin synthesis. with the aid of mathematical planning methods the optimal composition of the nutrient medium and optimal concentrations of its components have been selected. the cultivation of rh. delemar 1428 on the thus developed medium leads to the accumulation of 600 micrograms/1 biotin in the culture ... | 1983 | 6878207 |
| fungistatic mechanism of human transferrin for rhizopus oryzae and trichophyton mentagrophytes: alternative to simple iron deprivation. | human serum, human transferrin (tf), and the iron chelator 1,10-phenanthroline (op) produce iron-reversible fungistatic activity which has been attributed to simple iron deprivation. in this study, the influence of the size of the inoculum on the inhibitory activity of serum, tf, and op prepared with the same iron-binding capacity (2.5 micrograms/ml) for rhizopus oryzae and trichophyton mentagrophytes was examined. inhibition was monitored in liquid microcultures maintained at 37 degrees c and p ... | 1983 | 6885162 |
| increase in the activity of rhizopus delemar lipase on water-soluble esters by its binding with phosphatidylcholine. | rhizopus (rh.) delemar (atcc 34612) c-lipase was found to exhibit a slight activity towards water-soluble esters. the hydrolytic reaction of this lipase on alpha-naphthyl acetate was competitively inhibited by the presence of olive oil or tween 80. this finding showed that both substrates, insoluble triglyceride and water-soluble ester, were hydrolyzed at the same site on the enzyme. the activities on water-soluble esters (alpha-naphthyl acetate, beta-naphthyl acetate, methyl acetylsalicylate an ... | 1983 | 6885742 |
| nosocomical rhizopus infection (zygomycosis) in children. | three children with the rare occurrence of zygomycosis are descibed: two had involvement of a solitary lesion of gangrenous cellulitis on the buttocks, and th third was a neonate with gastric performation and a gangrenous appendicitis. all three patients were compromised hosts (two with leukemia and one a premature infant with respiratory distress syndrome). all three patients appeared to have acquired the same organism. rhizopus oryzae, from the same fomites, elastic bondages (elastoplast). the ... | 1980 | 6928943 |
| some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower (carthamus tinctorius) and linseed (linum ustatissimum). | 1. the average oil-body diameter in intact cells of developing linseed (linum usitatissimum) and safflower (carthamus tinctorius) cotyledons was similar (about 1.4 micrometer), and there was little change in size after oil bodies were isolated and repeatedly washed. 2. the glycerolipid composition of washed oil bodies from both developing and mature cotyledons of the two species was similar; oil bodies from ten different batches of cotyledons contained 4.3 +/- 0.16 mumol of 3-sn-phosphatidylchol ... | 1980 | 7008782 |
| antimycotic activity of berberine sulphate: an alkaloid from an indian medicinal herb. | berberine sulphate in concentrations of 10-25 mg ml -1 inhibited the growth of 11/13 fungi, viz. alternaria, aspergillus flavus, asp. fumigatus, candida albicans, curvularia, drechslera, fusarium, mucor, penicillium, rhizopus oryzae and scopulariopsis. concentrations of 50 mg ml -1 could check the growth of syncephalastrum as well but asp. niger remained unaffected. | 1982 | 7038929 |
| is platelet-activating factor (paf-acether) synthesis by murine peritoneal cells (pc) a two-step process? | paf-acether (1-o-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) is released from several cell sources simultaneously with an inactive non-acylated compound (1-o-alkyl-glyceryl-3-phosphorylcholine) (lyso-paf-acether). formation of the latter probably results from the activation of a phospholipase a2 (pla2). indeed, the pla2 inhibitors, bromophenacyl bromide (bpb), mepacrine, 874cb (100 microm), and edta (5 mm), blocked the zymosan-induced release of paf-acether from pc. edta and bpb also markedl ... | 1981 | 7053072 |
| fungistatic capacity of sera from guinea pigs injected with various iron solutions: differences between trichophyton mentagrophytes and rhizopus oryzae. | the fungistatic capacity and serum ion levels (si) of guinea pigs given subcutaneous injections of various iron solutions were examined. the administration of 2.0 ml of 0.1 m ferric ammonium sulfate, ferric sulfate, or ferric chloride subcutaneously had no significant effect on the si 3 h after administration, whereas ferric ammonium citrate, ferric citrate, or ferrous sulfate elevated the si to 50 to 140 times that necessary to saturate the unbound transferrin in normal sera. the sera from 11 o ... | 1982 | 7054128 |
| stability and inhibition of aspergillus and rhizopus lipases. | a lipase (triacylglyceral aclhydrolase, ec 3.1.1.3) secreted by aspergillus contains two chains each of molecular weight 25000, with 12 mannose, two galactose and two n-acetylglucosamine residues per chain. the average hydrophobicity is close to the mean fov globular proteins. thermal denaturation was biphasic when followed by circular dichroism but the stability is not unusual for globular proteins, nor does partial removal of the carbohydrate affect it. (delta g=46kj). refolding did not occur. ... | 1982 | 7055576 |
| monocyte-mediated damage to rhizopus oryzae hyphae in vitro. | clinicopathological correlations from human cases and experimental animal studies suggest that neutrophils are critical components of the host response to mucormycosis but that other cellular defense mechanisms appear to be important as well. since our previous studies demonstrated that rhizopus oryzae hyphae which are too large to be ingested completely can be damaged and probably killed by human neutrophils, we studied the antihyphal activity of human monocytes. as with neutrophils, light and ... | 1982 | 7141693 |
| [biotin formation by the fungus rhizopus delemar]. | the capability of 26 collection micromycete strains to synthesize biotin and transform destiobiotin was investigated. destiobiotin stimulated the biotin formation by the strains of the aspergillus genus (except a. sclerotiorum synthesizing vitamin traces), the fusarium genus, the rhizopus genus (except rh. nigricans), as well as thighemella hyalospora and sporotrichum sulfurescens. among the strains tested rhizopus delemar 1428 synthesized maximum quantities of biotin (over 300 micrograms/1). th ... | 1982 | 7145874 |
| susceptibility of zoopathogenic fungi to phytoalexins. | phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. they are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. employing a broth dilution technique, we have found the zoopathogens petriellidium boydii, aspergillus flavus, a. fumigatus, candida albicans, coccidioides immitis, crypto ... | 1980 | 7387136 |
| microbiological transformation of 1,2-diphenyl-3,5 dioxo-4-n-butyl pyrazolidine (phenylbutazone). | microbiological conversion of phenylbutazone with rhizopus arrhizus fischer gave 4-hydroxy phenylbutazone in excellent yield. spectral studies, mass spectrometry, in particular, were made of the obtained metabolite; other experiments performed with the intact microorganism or with the cell-free system, as well as studies with the well-known metabolic inhibitor, skf 525 a, indicated that the obtained 4-hydroxy phenylbutazone was not an artefact. similar experiments were performed on oxyphenbutazo ... | 1980 | 7389746 |
| fungi from interior organs of free-living small mammals in czechoslovakia and yugoslavia. | a total of 308 fungi was isolated from interior organs (lungs, spleen, liver) of 529 small mammals belonging to 21 species, 7 families and 3 orders (insectivora, chiroptera, rodentia), some of these being potentially pathogenic to vertebrates (e.g. aspergillus flavus, a. fumigatus, geotrichum candidum, mucor pusillus, rhizopus arrhizus). in one vole (microtus arvalis) captured in south moravia, adiaspiromycosis (emmonsia crescens) was demonstrated. comparison of mycoflora of hair and that of int ... | 1980 | 7419129 |
| the fumr gene encoding fumarase in the filamentous fungus rhizopus oryzae: cloning, structure and expression. | the filamentous fungus rhizopus oryzae (ro) is known for its ability to overproduce and accumulate high levels of fumaric acid (fa) under stress conditions. in order to study the molecular mechanisms involved in the increased biosynthesis of fa, the gene (designated fumr) encoding ro fumarase was cloned and analysed for its structure and expression. nucleotide (nt) sequence and comparison of the fumr product with fumarases from various sources established that fumr contains nine introns and enco ... | 1995 | 7557464 |
| acute rhinocerebral mucormycosis caused by rhizopus arrhizus from sri lanka. | rhinocerebral mucormycosis is a rare fungus infection reported mainly from the united states of america and europe. the disease is caused by zygomycete fungi, most often by a rhizopus species. diagnosis is often made post-mortem and in many instances culture identification of the fungus responsible has not been performed. a case of culturally proven rhinocerebral mucormycosis is described for the first time in sri lanka. the patient was a 56-year-old male who had been treated for diabetes mellit ... | 1995 | 7563267 |
| contribution of selected fungi to the reduction of cyanogen levels during solid substrate fermentation of cassava. | the effect of six individual strains of the dominant microflora in solid substrate fermenting cassava on cyanogen levels was examined. six out of eight batches of disinfected cassava root pieces were incubated for 72 h after inoculation with either of the fungi geotrichum candidum, mucor racemosus, neurospora sitophila, rhizopus oryzae and rhizopus stolonifer, or a bacillus sp., isolated from on-farm fermented cassava flours from uganda. one non-inoculated batch was incubated as a reference. lev ... | 1995 | 7577362 |
| effect of increasing inoculum sizes of pathogenic filamentous fungi on mics of antifungal agents by broth microdilution method. | inoculum size is a critical variable in development of methods for antifungal susceptibility testing for filamentous fungi. in order to investigate the influence of different inoculum sizes on mics of amphotericin b, 5-fluorocytosine, itraconazole, and miconazole, 32 clinical isolates (8 aspergillus fumigatus, 8 aspergillus flavus, 5 rhizopus arrhizus, 8 pseudallescheria boydii, and 3 fusarium solani isolates) were studied by the broth microdilution method. four inoculum sizes were studied: 1 x ... | 1995 | 7615745 |
| reducing cassava toxicity by heap-fermentation in uganda. | processing of cassava roots by the alur tribe in uganda includes a stage of solid substrate fermentation in heaps. changes in cyanogen levels during the process, microflora involved, and protein levels, amino acid patterns and mycotoxin contamination of the final products were studied. processing was monitored at six rural households and repeated at laboratory site, comparing it to sun-drying. flour samples from rural households were analysed for residual cyanogens, mutagenicity, cytotoxicity an ... | 1995 | 7621084 |
| inversion of lipase stereospecificity for fluorogenic alkyldiacyl glycerols. effect of substrate solubilization. | we synthesized enantiomeric 1-o-alkyl-2,3-diacyl-sn-glycerol and 3-o-alkyl-1,2-diacyl-sn-glycerol containing pyrene as a fluorescent reporter and the trinitrophenylamino residue as a fluorescence quencher; both reporter groups were covalently bound to the omega end of the acyl chains at positions sn-2 and sn-3(1), respectively. the fluorescence of the intact substrate molecules was very low. chemical or enzymic release of the fatty acyl chains lead to fluorescence dequenching. the rate of lipoly ... | 1995 | 7628484 |
| comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. | the purpose of the study was to evaluate the interlaboratory agreement of broth dilution susceptibility methods for five species of conidium-forming (size range, 2 to 7 microns) filamentous fungi. the methods used included both macro- and microdilution methods that were adaptations of the proposed reference method of the national committee for clinical laboratory standards for yeasts (m27-p). the mics of amphotericin b, fluconazole, itraconazole, miconazole, and ketoconazole were determined in s ... | 1995 | 7726488 |
| molecular and enzymatic properties of an aspartic proteinase from rhizopus hangchow. | an aspartic proteinase, rhizopuspepsin (ec 3.4.23.21), from rhizopus hangchow was purified. the m(r) and isoelectric point were determined as ca 37,000 and 4.5, respectively. the first 19 amino acids in the n-terminal region were sgsgvvpmtdyeydieyyg. the contents of the alpha-helix, beta-structure and random coil were calculated to be ca 7.5, 88.9 and 2.7%, respectively. the enzyme can activate trypsinogen at ph 3.0. the activity was completely inactivated by pepstatin a. the specificity and mod ... | 1995 | 7766059 |
| electrophoretic free mobility and viability of microbial cells: a preliminary study in preparation for space experiments. | electrophoretic free mobilities (efm) of four fungal spores and five bacterial cells were determined in 7 mm triethanolamine/acetate (tea) buffer by means of microscopic electrophoresis (me) and free flow electrophoresis (ffe). spores of aspergillus terreus, penicillium citrinum, gliocladium virens, and rhizopus oryzae had similar efm from 2.2 to 3.1 microns sec-1/v cm-1. the resolution of the spore mixture by ffe was therefore determined to be poor. bacterial cells of salmonella typhimurium lt2 ... | 1994 | 7880880 |
| cerebral mucormycosis associated with intravenous drug use: three case reports and review. | we describe three cases of cerebral mucormycosis in intravenous drug users and review 22 previously reported cases. involvement of the basal ganglia was demonstrated in all but two cases. seven of the 10 patients tested for antibodies to the human immunodeficiency virus (hiv) were seronegative. eight of the 25 patients survived and were discharged from the hospital; for 7 of 10 patients, cultures of brain lesions yielded rhizopus arrhizus. the radiographic findings varied, and in most cases, no ... | 1994 | 7888545 |
| improvement of heavy metal biosorption by mycelial dead biomasses (rhizopus arrhizus, mucor miehei and penicillium chrysogenum): ph control and cationic activation. | fungal mycelial by-products from fermentation industries present a considerable affinity for soluble metal ions (e.g. zn, cd, ni, pb, cr, ag) and could be used in biosorption processes for purification of contaminated effluents. in this work the influence of ph on sorption parameters is characterized by measuring the isotherms of five heavy metals (ni, zn, cd, ag and pb) with rhizopus arrhizus biomass under ph-controlled conditions. the maximum sorption capacity for lead was observed at ph 7.0 ( ... | 1994 | 7917420 |
| current progress in crystallographic studies of new lipases from filamentous fungi. | lipases from filamentous fungi have been studied extensively over many years. they exhibit properties attractive for industrial applications, e.g. in laundry detergents, tanning and paper industries and stereospecific organic synthesis. enzymes from the fungi rhizomucor miehei and geotrichum candidum have been among the first neutral lipases to be characterized structurally by x-ray diffraction methods. in this paper we report a preliminary account of crystallographic studies of three other fung ... | 1994 | 8029211 |
| immunohistochemical identification of aetiological agents of systemic bovine zygomycosis. | the reactivity of a panel of rabbit hyperimmune antisera raised against somatic antigens of three zygomycetes, absidia corymbifera, rhizopus oryzae and rhizomucor (mucor) pusillus was assessed by crossed immunoelectrophoresis (xie) and indirect immunofluorescence (iif) staining of experimentally infected murine tissues. despite a widespread cross-reactivity of the neat antisera, heterologous absorption rendered antisera monospecific as assessed by xie. heterologous absorption also rendered the a ... | 1994 | 8040374 |
| the normal mycoflora of commodities from thailand. 1. nuts and oilseeds. | a comprehensive study was carried out of the fungi occurring in commodities normally traded in thailand. samples of major commodities were obtained from farmers' stocks and middlemen in major producing areas throughout the country. retail samples were obtained from outlets in and around bankok. samples were divided into two portions, one being examined in bangkok, and the second in sydney. after surface disinfection, fungi were enumerated by direct plating on dichloran rose bengal chloramphenico ... | 1993 | 8110599 |
| crystallization and preliminary crystallographic studies of the precursor and mature forms of a neutral lipase from the fungus rhizopus delemar. | a neutral lipase from the filamentous fungus rhizopus delemar has been crystallized in both its proenzyme and mature forms. although the latter crystallizes readily and produces a variety of crystal forms, only one was found to be suitable for x-ray studies. it is monoclinic (c2, a = 92.8 a, b = 128.9 a, c = 78.3 a, beta = 135.8) with two molecules in the asymmetric unit related by a noncrystallographic diad. the prolipase crystals are orthorhombic (p2(1)2(1)2(1), with a = 79.8 a, b = 115.2 a, c ... | 1994 | 8202471 |
| overexpression of a rhizopus delemar lipase gene in escherichia coli. | a cloned complementary deoxyribonucleic acid encoding the precursor polypeptide of an extracellular lipase from the fungus rhizopus delemar was altered by site-directed mutagenesis to generate deoxyribonucleic acid fragments that specifically code for the polypeptides of the proenzyme and the mature form of the lipase. attempts to produce these polypeptides in enzymatically active form in escherichia coli revealed toxic effects toward the host. therefore the polypeptides were expressed as inacti ... | 1993 | 8441342 |
| release of volatile branched-chain and other fatty acids from ruminant milk fats by various lipases. | bovine, ovine, and caprine milk fats were treated with pregastric lipases (kid goat, calf, and lamb), microbial lipases (candida cylindracea, c. cylindracea ay30, aspergillus niger apf12, rhizopus arrhizus, penicillium roqueforti r10, and mucor zavanicus map 10), porcine pancreatic lipase, or milk lipase. all three pregastric lipases preferentially hydrolyzed volatile branched-chain and short n-chain fatty acids from each milk fat. pregastric lipases also released a relatively low proportion of ... | 1993 | 8463483 |
| recovery from rhinocerebral mucormycosis in a ketoacidotic diabetic patient: a case report. | an 18-year-old woman with insulin-dependent diabetes mellitus developed an infection of the paranasal sinuses with rhizopus oryzae resulting in facial swelling, hemiplegia and blindness of the right eye. the therapy of this rhinocerebral mucormycosis consisted of extensive surgical debridement, administration of high-dose amphotericin b, hyperbaric oxygen and control of the underlying predisposing diabetes mellitus. the patient eventually recovered with however, the loss of one eye. | 1993 | 8509703 |
| improved detection of medically important fungi by immunoperoxidase staining with polyclonal antibodies. | this study was performed to identify pathological fungi of eight species [aspergillus fumigatus, candida albicans, torulopsis (candida) glabrata, cryptococcus neoformans, fusarium anthophilum, rhizopus oryzae, sporothrix schenckii and trichosporon beigelii] in formalin-fixed, paraffin-embedded tissue sections by indirect immunoperoxidase staining. mature albino rabbits were immunized with formalin-killed organisms. antibodies were prepared by precipitation. immunoperoxidase staining was applied ... | 1995 | 8548126 |
| degradation of stachyose, raffinose, melibiose and sucrose by different tempe-producing rhizopus fungi. | forty-six strains of tempe-forming rhizopus species were screened for their ability to grow on raffinose as the sole carbon source. six of the strains showed good growth and sporulation. these isolates were one rhizopus oligosporus, one rhizopus microsporus var. chinensis, three rhizopus oryzae and one rhizopus stolonifer. these six moulds and r. oligosporus strain nrrl 2710 were investigated for their metabolism of the raffinose family of alpha-galactoside carbohydrates. degradation experiments ... | 1995 | 8579835 |
| pcr and single-strand conformational polymorphism for recognition of medically important opportunistic fungi. | the application of pcr technology to molecular diagnostics holds great promise for the early identification of medically important pathogens. pcr has been shown to be useful for the detection of the presence of fungal dna in both laboratory and clinical samples. considerable interest has been focused on the utility of selecting universal primers, those that recognize constant regions among most, if not all, medically important fungi. once an amplicon, or piece of amplified dna determined by the ... | 1995 | 8586705 |
| influence of fungi associated with bananas on nutritional content during storage. | botryodiplodia theobromae, rhizopus oryzae, aspergillus niger, a. flavus and fusarium equiseti were found to be associated with the ripening of bananas and also caused rot during storage. bananas stored in baskets with ash fire wood ripened 2-3 days earlier than bananas stored in fibre sacks and under constant light. the infected bananas showed a decrease in the quantity of total soluble sugars, protein, lipid, crude fibre, ash, ascorbic acid and mineral elements when compared with the control f ... | 1996 | 8711953 |
| [rhino-orbito-cerebral mucormycosis caused by rhizopus oryzae.a typical case in a cirrhotic patient]. | the rhino-orbito-cerebral mucormycosis is an acute rapidly progressive fungal infection. this disease is caused by a zygomycetes fungus, most often from the rhizopus genus. this fungus is saprophitic of the nasal cavity and paranasal sinuses. it becomes pathogenic in some particular conditions, specially during diabetes mellitus. histopathological study is the only method allowing the diagnosis, by revealing the tissue invasion by characteristic hyphae. mycologic study allows a definitive identi ... | 1993 | 8397543 |
| molecular characterization of an extracellular acid-resistant lipase produced by rhizopus javanicus. | an extracellular lipase (triacylglycerol acylhydrolase ec 3.1.1.3), produced by the fungus rhizopus javanicus was purified to homogeneity using an expeditious two-step isolation method. the enzyme, with a molecular mass of 36 kda and a specific activity of 9260 microequivalent of fatty acid released per minute and mg under standard conditions, consists of three isoforms with isoelectric points of 7.8, 7.7, and 7.1, respectively. the purified lipase was digested using chemical and enzymatical pro ... | 1993 | 8329142 |
| orbital infections in patients with human immunodeficiency virus infection. | opportunistic infections frequently involve the anterior and posterior segments of the eye but rarely occur in the orbit in patients with human immunodeficiency virus (hiv) infection. the authors managed eight patients with hiv and unilateral orbital infections who presented between july 1988 and march 1995. | 1996 | 8841310 |
| rapid, sensitive fluorometric determination of serum triglyceride by measuring lipase-liberated fatty acids. | a continuous fluorescence displacement assay was developed for the measurement of long-chain fatty acids and utilized in the study of triglyceride lipase-catalyzed reactions (wilton, dc. biochem j 1991;276:129-33). we now describe a method for the rapid measurement of triglyceride in serum with the fluorescence displacement assay. the method involves the hydrolysis of a diluted sample equivalent to 0.1 microl of original serum with excess lipase (ec 3.1.1.3) from rhizopus arrhizus and measuring ... | 1994 | 8287522 |
| gastric mucormycosis due to rhizopus oryzae in a renal transplant recipient. | gastric mucormycosis is a rare disease with a reported fatal outcome of 98%. manifestations range from colonization of peptic ulcers to infiltrative disease with vascular invasion and dissemination. in our renal transplant patient a deep gastric ulceration infected with rhizopus oryzae (class zygomycetes), which is known to be an agent of mucormycosis, was diagnosed in the early posttransplant period after antirejection therapy. the infection was successfully managed with amphotericin b and omep ... | 1996 | 8880524 |
| production of extracellular acidic lipase by rhizopus arrhizus as a function of culture conditions. | thermophilic strain of rhizopus arrhizus accumulates an acidic lipase in culture fluid when grown in a medium containing ground nut oil, milk powder and inorganic salts. addition of 2.0% ground nut oil yielded the highest productivity of enzyme. soyabean meal and arabinose were found to be the best nitrogen and carbon sources for enzyme production respectively. addition of metal ions such as mncl2, sncl2 and cacl2 increased the enzyme productivity by 4 fold. the enzyme productivity in the fermen ... | 1993 | 8181953 |
| zygomycosis and hiv infection. | the severe immunosuppression associated with hiv infection increases susceptibility to opportunistic fungi. we describe a primary gangrenous cutaneous infection caused by rhizopus arrhizus in an hiv-infected intravenous narcotic user. in addition, we review nine reported cases of zygomycosis in hiv-infected patients and discuss the frequency and outcome of zygomycosis in hiv infection. eight of 10 patients were intravenous drug users. cutaneous infection occurred in four patients. another case w ... | 1994 | 8169272 |
| alteration of chain length selectivity of a rhizopus delemar lipase through site-directed mutagenesis. | the coding sequences of the rhizopus delemar lipase and prolipase were altered by oligonucleotide-directed mutagenesis to introduce amino acid substitutions. the resulting mutant enzymes, synthesized by the bacterial host escherichia coli bl21 (de3), were tested for their ability to hydrolyze the triglycerides triolein (to), tricaprylin (tc) and tributyrin (tb). mutagenesis and lipase gene expression were carried out using plasmid vectors derived from previously described recombinant plasmids [j ... | 1994 | 8090057 |
| immunoperoxidase staining for identification of aspergillus species in routinely processed tissue sections. | to evaluate the performance of an immunoperoxidase stain using the monoclonal antibody eb-a1 to detect aspergillus species in formalin fixed, paraffin wax embedded tissue. | 1996 | 8943743 |