| a genome-wide analysis of rna pseudoknots that stimulate efficient -1 ribosomal frameshifting or readthrough in animal viruses. | programmed -1 ribosomal frameshifting (prf) and stop codon readthrough are two translational recoding mechanisms utilized by some rna viruses to express their structural and enzymatic proteins at a defined ratio. efficient recoding usually requires an rna pseudoknot located several nucleotides downstream from the recoding site. to assess the strategic importance of the recoding pseudoknots, we have carried out a large scale genome-wide analysis in which we used an in-house developed program to d ... | 2013 | 24298557 |
| molecular mechanisms involved in the pathogenesis of alphavirus-induced arthritis. | arthritogenic alphaviruses, including ross river virus (rrv), chikungunya virus (chikv), sindbis virus (sinv), mayaro virus (mayv), o'nyong-nyong virus (onnv), and barmah forest virus (bfv), cause incapacitating and long lasting articular disease/myalgia. outbreaks of viral arthritis and the global distribution of these diseases point to the emergence of arthritogenic alphaviruses as an important public health problem. this review discusses the molecular mechanisms involved in alphavirus-induced ... | 2013 | 24069610 |
| dendritic cell immunoreceptor regulates chikungunya virus pathogenesis in mice. | chikungunya virus (chikv) is a mosquito-borne alphavirus responsible for recent epidemic outbreaks of debilitating disease in humans. alphaviruses are known to interact with members of the c-type lectin receptor family of pattern recognition proteins, and given that the dendritic cell immunoreceptor (dcir) is known to act as a negative regulator of the host inflammatory response and has previously been associated with rheumatoid arthritis, we evaluated dcir's role in response to chikv infection. ... | 2013 | 23487448 |
| a tyrosine-to-histidine switch at position 18 of the ross river virus e2 glycoprotein is a determinant of virus fitness in disparate hosts. | arthritogenic alphaviruses are human pathogens maintained in nature through alternating replication in vertebrates and mosquitoes. using chimeric viruses, we previously reported that replacement of the pe2 coding region of the t48 strain of ross river virus (rrv-t48) with that from the attenuated dc5692 strain, which differ by 7 amino acids, resulted in an attenuated disease phenotype in a mouse model of rrv-induced rheumatic disease. here, we demonstrate that introduction of one of these amino ... | 2013 | 23514884 |
| adaptive changes in alphavirus mrna translation allowed colonization of vertebrate hosts. | members of the alphavirus genus are arboviruses that alternate replication in mosquitoes and vertebrate hosts. in vertebrate cells, the alphavirus resists the activation of antiviral rna-activated protein kinase (pkr) by the presence of a prominent rna structure (downstream loop [dlp]) located in viral 26s transcripts, which allows an eif2-independent translation initiation of these mrnas. this article shows that dlp structure is essential for replication of sindbis virus (sinv) in vertebrate ce ... | 2012 | 22761388 |
| cell-based analysis of chikungunya virus e1 protein in membrane fusion. | chikungunya fever is a pandemic disease caused by the mosquito-borne chikungunya virus (chikv). e1 glycoprotein mediation of viral membrane fusion during chikv infection is a crucial step in the release of viral genome into the host cytoplasm for replication. how the e1 structure determines membrane fusion and whether other chikv structural proteins participate in e1 fusion activity remain largely unexplored. | 2012 | 22520648 |
| reemergence of chikungunya virus in indian subcontinent. | chikungunya virus (chikv), a reemerging arboviral disease of public health concern is characterized by a triad of fever, rash and arthralgia. it was responsible for a number of epidemics in asia and africa. the severity of the current epidemic can be judged by the fact that an estimated 1.38 million people in india and one-third of the la reunion population (by april 2006) were affected by chikv. aedes aegypti and aedes albopictus are the major mosquitoes transmitting chikv in asia. various neur ... | 2010 | 23637474 |
| [arbovirus causing hemorrhagic fever at imss]. | to know the arbovirus causing hemorrhagic fever in patients at the mexican institute of social security. | 2006 | 16904038 |
| long-term arthralgia after mayaro virus infection correlates with sustained pro-inflammatory cytokine response. | mayaro virus (mayv), an alphavirus similar to chikungunya virus (chikv), causes an acute debilitating disease which results in the development of long-term arthralgia in more than 50% of infected individuals. currently, the immune response and its role in the development of mayv-induced persistent arthralgia remain unknown. in this study, we evaluated the immune response of individuals with confirmed mayv infection in a one-year longitudinal study carried out in loreto, peru. we report that mayv ... | 2015 | 26496497 |
| effect of hypertonic medium on the protein synthesis in l-a9 and aedes albopictus cells infected with mayaro virus. | | 1991 | 1668789 |
| genome analysis of mayaro virus imported to germany from french guiana. | | 0 | 24960052 |
| approaches to the treatment of disease induced by chikungunya virus. | chikungunya virus, a re-emerging mosquito-borne alphavirus, causes fever, rash and persistent arthralgia/arthritis in humans. severe outbreaks have occurred resulting in infections of millions of people in southeast asia and africa. currently there are no antiviral drugs or vaccines for prevention and treatment of chikungunya infections. herein we report the current status of research on antiviral drugs and vaccines for chikungunya virus infections. | 0 | 24434329 |
| serological evidence of flaviviruses and alphaviruses in livestock and wildlife in trinidad. | seroprevalence rates of selected arboviruses in animal populations in trinidad were determined using serum samples collected between 2006 and 2009 from horses (n=506), cattle (n=163), sheep (n=198), goats (n=82), pigs (n=184), birds (n=140), rodents (n=116), and other vertebrates (n=23). the sera were screened for antibodies to west nile virus (wnv), st. louis encephalitis virus (slev), ilheus virus (ilhv), bussuquara virus (bsqv), venezuelan equine encephalitis virus (veev), eastern equine ence ... | 2012 | 22989182 |
| an rna trapping mechanism in alphavirus mrna promotes ribosome stalling and translation initiation. | during translation initiation, eukaryotic initiation factor 2 (eif2) delivers the met-trna to the 40s ribosomal subunit to locate the initiation codon (augi) of mrna during the scanning process. stress-induced eif2 phosphorylation leads to a general blockade of translation initiation and represents a key antiviral pathway in mammals. however, some viral mrnas can initiate translation in the presence of phosphorylated eif2 via stable rna stem-loop structures (dlp; downstream loop) located in thei ... | 2016 | 26984530 |
| viral arthritis. | acute-onset arthritis is a common clinical problem facing both the general clinician and the rheumatologist. a viral aetiology is though to be responsible for approximately 1% of all cases of acute arthritis with a wide range of causal agents recognised. the epidemiology of acute viral arthritis continues to evolve, with some aetiologies, such as rubella, becoming less common due to vaccination, while some vector-borne viruses have become more widespread. a travel history therefore forms an impo ... | 0 | 27037381 |
| multiple immune factors are involved in controlling acute and chronic chikungunya virus infection. | the recent epidemic of the arthritogenic alphavirus, chikungunya virus (chikv) has prompted a quest to understand the correlates of protection against virus and disease in order to inform development of new interventions. herein we highlight the propensity of chikv infections to persist long term, both as persistent, steady-state, viraemias in multiple b cell deficient mouse strains, and as persistent rna (including negative-strand rna) in wild-type mice. the knockout mouse studies provided evid ... | 2014 | 25474568 |
| chikungunya virus-vector interactions. | chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya fever, a severe, debilitating disease that often produces chronic arthralgia. since 2004, chikv has emerged in africa, indian ocean islands, asia, europe, and the americas, causing millions of human infections. central to understanding chikv emergence is knowledge of the natural ecology of transmission and vector infection dynamics. this review presents current understanding of chikv infection dynamics in mosquito v ... | 2014 | 25421891 |
| seroprevalence of alphavirus antibodies in a cross-sectional study in southwestern tanzania suggests endemic circulation of chikungunya. | to date, alphavirus infections and their most prominent member, chikungunya fever, a viral disease which first became apparent in tanzania in 1953, have been very little investigated in regions without epidemic occurrence. few data exist on burden of disease and socio-economic and environmental covariates disposing to infection. | 2014 | 25079964 |
| attenuating mutations in nsp1 reveal tissue-specific mechanisms for control of ross river virus infection. | ross river virus (rrv) is one of a group of mosquito-transmitted alphaviruses that cause debilitating, and often chronic, musculoskeletal disease in humans. previously, we reported that replacement of the nonstructural protein 1 (nsp1) gene of the mouse-virulent rrv strain t48 with that from the mouse-avirulent strain dc5692 generated a virus that was attenuated in a mouse model of disease. here we find that the six nsp1 nonsynonymous nucleotide differences between strains t48 and dc5692 are det ... | 2014 | 24429363 |
| occurrence and correlates of symptom persistence following acute dengue fever in peru. | dengue virus (denv) infection causes an acute febrile illness generally considered to result in either complete recovery or death. some reviews describe persistent symptoms after the febrile phase, although empirical data supporting this phenomenon is scarce. we evaluated symptom persistence in acute febrile denv-infected and denv-negative (controls) individuals from peru. self-reported solicited symptoms were evaluated at an acute and a follow-up visit, occurring 10-60 days after symptom onset. ... | 2014 | 24470564 |
| identification of novel compounds inhibiting chikungunya virus-induced cell death by high throughput screening of a kinase inhibitor library. | chikungunya virus (chikv) is a mosquito-borne arthrogenic alphavirus that causes acute febrile illness in humans accompanied by joint pains and in many cases, persistent arthralgia lasting weeks to years. the re-emergence of chikv has resulted in numerous outbreaks in the eastern hemisphere, and threatens to expand in the foreseeable future. unfortunately, no effective treatment is currently available. the present study reports the use of resazurin in a cell-based high-throughput assay, and an i ... | 2013 | 24205414 |
| a fully attenuated recombinant salmonid alphavirus becomes pathogenic through a single amino acid change in the e2 glycoprotein. | a recombinant sleeping disease virus (rsdv) was previously shown to be totally attenuated and provide long-term protection in trout (c. moriette, m. leberre, a. lamoureux, t. l. lai, m. brémont, j. virol. 80:4088-4098, 2006). sequence comparison of the rsdv to wild-type genomes exhibited a number of nucleotide changes. in the current study, we demonstrate that the virulent phenotype of sdv was essentially associated with two amino acid changes, v8a and m136t, in the e2 glycoprotein, with the v8a ... | 2013 | 23449806 |
| inhibition of mayaro virus replication by prostaglandin a(1) in vero cells. | prostaglandins exhibit antiviral activity against a wide variety of rna and dna viruses. in the present report, we describe the effect of cyclopentenone prostaglandin a(1) (pga(1)) on mayaro virus replication in vero cells. virus yield was significantly reduced at nontoxic concentrations which did not suppress dna, rna or protein synthesis in uninfected or infected cells. antiviral action decreased if pga(1) was added at later times after infection. in mayaro virus-infected cells, pga(1) inhibit ... | 2001 | 11805440 |
| mayaro virus infection cycle relies on casein kinase 2 activity. | replication of mayaro virus in vero cells induces dramatic cytopathic effects and cell death. in this study, we have evaluated the role of casein kinase 2 (ck2) during mayaro virus infection cycle. we found that ck2 was activated during the initial stages of infection ( approximately 36% after 4h). this activation was further confirmed when the enzyme was partially purified from the cellular lysate either by mono q 5/5hr column or heparin-agarose column. using this later column, we found that th ... | 2002 | 12207921 |
| factors shaping the adaptive landscape for arboviruses: implications for the emergence of disease. | many examples of the emergence or re-emergence of infectious diseases involve the adaptation of zoonotic viruses to new amplification hosts or to humans themselves. these include several instances of simple mutational adaptations, often to hosts closely related to the natural reservoirs. however, based on theoretical grounds, arthropod-borne viruses, or arboviruses, may face several challenges for adaptation to new hosts. here, we review recent findings regarding adaptive evolution of arboviruse ... | 0 | 23374123 |
| genetic ablation of arginase 1 in macrophages and neutrophils enhances clearance of an arthritogenic alphavirus. | chikungunya virus (chikv) and ross river virus (rrv) cause a debilitating, and often chronic, musculoskeletal inflammatory disease in humans. macrophages constitute the major inflammatory infiltrates in musculoskeletal tissues during these infections. however, the precise macrophage effector functions that affect the pathogenesis of arthritogenic alphaviruses have not been defined. we hypothesized that the severe damage to musculoskeletal tissues observed in rrv- or chikv-infected mice would pro ... | 2012 | 22972923 |
| genomic characterization of a novel virus of the family tymoviridae isolated from mosquitoes. | the family tymoviridae comprises three plant virus genera, including tymovirus, marafivirus, and maculavirus, which are found in most parts of the world and cause severe agricultural losses. we describe a putatively novel member of the family tymoviridae, which is isolated from mosquitoes (culex spp.), referred to as cutlv. | 2012 | 22848363 |
| mayaro virus in child with acute febrile illness, haiti, 2015. | mayaro virus has been associated with small outbreaks in northern south america. we isolated this virus from a child with acute febrile illness in rural haiti, confirming its role as a cause of mosquitoborne illness in the caribbean region. the clinical presentation can mimic that of chikungunya, dengue, and zika virus infections. | 0 | 27767924 |
| mrna capping by venezuelan equine encephalitis virus nsp1: functional characterization and implications for antiviral research. | alphaviruses are known to possess a unique viral mrna capping mechanism involving the viral nonstructural protein nsp1. this enzyme harbors methyltransferase (mtase) and nsp1 guanylylation (gt) activities catalyzing the transfer of the methyl group from s-adenosylmethionine (adomet) to the n7 position of a gtp molecule followed by the formation of an m(7)gmp-nsp1 adduct. subsequent transfer of m(7)gmp onto the 5' end of the viral mrna has not been demonstrated in vitro yet. here we report the bi ... | 2015 | 26041283 |
| bovine lactoferrin inhibits toscana virus infection by binding to heparan sulphate. | toscana virus is an emerging sandfly-borne bunyavirus in mediterranean europe responsible for neurological diseases in humans. it accounts for about 80% of paediatric meningitis cases during the summer. despite the important impact of toscana virus infection-associated disease on human health, currently approved vaccines or effective antiviral treatments are not available. in this research, we have analyzed the effect of bovine lactoferrin, a bi-globular iron-binding glycoprotein with potent ant ... | 2015 | 25643293 |
| picornavirus morphogenesis. | the picornaviridae represent a large family of small plus-strand rna viruses that cause a bewildering array of important human and animal diseases. morphogenesis is the least-understood step in the life cycle of these viruses, and this process is difficult to study because encapsidation is tightly coupled to genome translation and rna replication. although the basic steps of assembly have been known for some time, very few details are available about the mechanism and factors that regulate this ... | 0 | 25184560 |
| mitochondrial bioenergetic alterations in mouse neuroblastoma cells infected with sindbis virus: implications to viral replication and neuronal death. | the metabolic resources crucial for viral replication are provided by the host. details of the mechanisms by which viruses interact with host metabolism, altering and recruiting high free-energy molecules for their own replication, remain unknown. sindbis virus, the prototype of and most widespread alphavirus, causes outbreaks of arthritis in humans and serves as a model for the study of the pathogenesis of neurological diseases induced by alphaviruses in mice. in this work, respirometric analys ... | 2012 | 22485151 |
| differential unfolded protein response during chikungunya and sindbis virus infection: chikv nsp4 suppresses eif2α phosphorylation. | chikungunya (chikv) and sindbis (sinv) are arboviruses belonging to the alphavirus genus within the togaviridae family. they cause frequent epidemics of febrile illness and long-term arthralgic sequelae that affect millions of people each year. both viruses replicate prodigiously in infected patients and in vitro in mammalian cells, suggesting some level of control over the host cellular translational machinery that senses and appropriately directs the cell's fate through the unfolded protein re ... | 2013 | 23356742 |
| pathogenic characterization of a cervical lymph node derived from a patient with kawasaki disease. | kawasaki disease (kd) is the most common cause of multisystem vasculitis in childhood. although cervical lymphadenitis is one of the major symptoms in kd, lymph node biopsy is rarely performed, because kd is usually diagnosed by clinical symptoms. a cervical lymph node biopsy was taken from a girl aged 1 year and 8 months who had suspected lymphoma, but she was diagnosed with kd after the biopsy. the cervical lymph node specimen was analyzed with multivirus real-time pcr that can detect >160 vir ... | 2012 | 23071864 |
| development of an algorithm for production of inactivated arbovirus antigens in cell culture. | arboviruses are medically important pathogens that cause human disease ranging from a mild fever to encephalitis. laboratory diagnosis is essential to differentiate arbovirus infections from other pathogens with similar clinical manifestations. the arboviral diseases branch (adb) reference laboratory at the cdc division of vector-borne diseases (dvbd) produces reference antigens used in serological assays such as the virus-specific immunoglobulin m antibody-capture enzyme-linked immunosorbent as ... | 2014 | 25102428 |
| full inactivation of human influenza virus by high hydrostatic pressure preserves virus structure and membrane fusion while conferring protection to mice against infection. | whole inactivated vaccines (wivs) possess greater immunogenicity than split or subunit vaccines, and recent studies have demonstrated that wivs with preserved fusogenic activity are more protective than non-fusogenic wivs. in this work, we describe the inactivation of human influenza virus x-31 by high hydrostatic pressure (hhp) and analyze the effects on the structure by spectroscopic measurements, light scattering, and electron microscopy. we also investigated the effects of hhp on the glycopr ... | 2013 | 24282553 |
| mayaro virus infection, amazon basin region, peru, 2010-2013. | during 2010-2013, we recruited 16 persons with confirmed mayaro virus infection in the peruvian amazon to prospectively follow clinical symptoms and serologic response over a 12-month period. mayaro virus infection caused long-term arthralgia in more than half, similar to reports of other arthritogenic alphaviruses. | 0 | 24210165 |
| searching for putative avian malaria vectors in a seasonally dry tropical forest in brazil. | haemosporidian parasites of the genera plasmodium and haemoproteus can have detrimental effects on individual birds and populations. despite recent investigations into the distribution and richness of these parasites and their vertebrate hosts, little is known about their dipteran vectors. the neotropics has the highest diversity of mosquitoes in the world, but few studies have tried to identify vectors in this area, hampering the understanding of the ecology of avian malaria in the highly diver ... | 2016 | 27852326 |
| una virus: first report of human infection in argentina. | una virus (unav), togaviridae family, is widely distributed in south america, where infections have been detected in mosquitoes and vertebrate hosts (humans, birds and horses). we analyzed human sera from córdoba inhabitants aged 44 to 89 years and using a neutralization test, we found a prevalence of unav antibodies of 3.8% (3/79). the low titers detected suggest past infections probably acquired in rural areas of the province of córdoba (central argentina). none sera were found positive for ma ... | 2003 | 12754579 |
| rickettsial disease in the peruvian amazon basin. | using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ... | 2016 | 27416029 |
| chikungunya outbreak in a rural area of western cameroon in 2006: a retrospective serological and entomological survey. | abstract: | 2010 | 20444282 |
| genetic diversity of venezuelan alphaviruses and circulation of a venezuelan equine encephalitis virus subtype iab strain during an interepizootic period. | several species of alphaviruses have been previously described in the americas, some of which are associated with encephalitis and others are associated with arthralgia. venezuelan equine encephalitis virus (veev) and eastern equine encephalitis virus (eeev) are endemic to venezuela, with the former being responsible for major outbreaks of severe and often fatal disease in animals and humans. the aim of this study was to analyze the genetic diversity of venezuelan alphaviruses isolated during tw ... | 2015 | 25940191 |
| infection with mayaro virus in a french traveller returning from the amazon region, brazil, january, 2010. | mayaro virus (mayv) disease is a mosquito-borne zoonosis endemic in humid forests of tropical south america. mayv is closely related to other alphaviruses that produce a dengue-like illness accompanied by long-lasting arthralgia. a french tourist developed high-grade fever and severe joint manifestations following a 15-day trip in the amazon basin, brazil, and was diagnosed with mayv infection in january 2010. this case is the first reported in a traveller returning from an endemic south america ... | 2010 | 20460093 |
| ilheus virus infection in human, bolivia. | | 0 | 22377378 |
| imported mayaro virus infection in the netherlands. | a dutch couple, presenting with persisting arthralgias, temporary fever and rash after a stay in surinam were diagnosed with mayaro virus infection. mayaro virus is a relatively unknown south american alphavirus responsible for dengue-like clinical features and persisting arthralgias. an important, but probably underappreciated cross-reactivity with other alphaviruses like chikungunya virus is present, which may become of clinical importance in the event the various alphaviruses will have overla ... | 2010 | 20600300 |
| mayaro virus infection in amazonia: a multimodel inference approach to risk factor assessment. | arboviral diseases are major global public health threats. yet, our understanding of infection risk factors is, with a few exceptions, considerably limited. a crucial shortcoming is the widespread use of analytical methods generally not suited for observational data--particularly null hypothesis-testing (nht) and step-wise regression (swr). using mayaro virus (mayv) as a case study, here we compare information theory-based multimodel inference (mmi) with conventional analyses for arboviral infec ... | 2012 | 23071852 |
| phospholipase a2 isolated from the venom of crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope. | the flaviviridae family includes several virus pathogens associated with human diseases worldwide. within this family, dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. currently, there are no vaccines or specific antiviral drugs against dengue virus or against most of the viruses of this family. therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses ... | 2014 | 25383618 |
| a mouse model of chikungunya virus-induced musculoskeletal inflammatory disease: evidence of arthritis, tenosynovitis, myositis, and persistence. | chikungunya virus (chikv), an emerging mosquito-borne alphavirus, causes debilitating rheumatic disease in humans that can last for weeks to months. starting in 2004, a chikv outbreak in the indian ocean region affected millions of people, and infected travelers introduced chikv to new regions. the pathogenesis of chikv is poorly understood, and no approved vaccines or specific therapies exist. a major challenge to the study of chikv disease is the lack of a small animal model that recapitulates ... | 2010 | 21224040 |
| celebration of 60th anniversary of brazilian society for microbiology. | | 2016 | 27865633 |
| why arboviruses can be neglected tropical diseases. | | 2008 | 18575597 |
| [exotic viral arthritis: role of alphavirus]. | only six of the many alphavirus known to affect humans can cause articular manifestations. they are the ross river and barmah forest viruses from the south pacific, the chikungunya, o'nyong nyong and sindbis viruses from tropical africa, and the mayaro virus from south america. in most cases, articular manifestations involve arthralgia or transient arthritis and are usually minor. however in some cases especially involving ross river virus acute polyarthritis may be the most prominent clinical f ... | 2004 | 15224565 |
| an extract from taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro. | influenza virus remains an emerging virus and causes pandemics with high levels of fatality. after screening different plant extracts with potential anti-influenza activity, a water extract of taxodium distichum stems (tdswex) showed excellent activity against influenza viruses. the ec50 of tdswex was 0.051 ± 0.024 mg/ml against influenza virus a/wsn/33. tdswex had excellent antiviral efficacy against various strains of human influenza a and b viruses, particularly oseltamivir-resistant clinical ... | 2016 | 27796330 |
| dna microarray platform for detection and surveillance of viruses transmitted by small mammals and arthropods. | viruses transmitted by small mammals and arthropods serve as global threats to humans. most emergent and re-emergent viral agents are transmitted by these groups; therefore, the development of high-throughput screening methods for the detection and surveillance of such viruses is of great interest. in this study, we describe a dna microarray platform that can be used for screening all viruses transmitted by small mammals and arthropods (smaviruschip) with nucleotide sequences that have been depo ... | 2016 | 27654806 |
| mayaro virus and dengue virus 1 and 4 natural infection in culicids from cuiabá, state of mato grosso, brazil. | this study aimed to verify the diversity of culicidae species and their frequency of infection with flaviviruses and alphaviruses in cuiabá, state of mato grosso, brazil. mosquitoes were captured with nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (rt-pcr) for 11 flavivirus and five alphavirus. p ... | 0 | 26784852 |
| clinical and virological descriptive study in the 2011 outbreak of dengue in the amazonas, brazil. | dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito aedes aegypti. in the state of amazonas, brazil during the 2011 outbreak of dengue all the four dengue virus (denv) serotypes circulating simultaneously were observed. the aim of the study was to describe the clinical epidemiology of dengue in manaus, the capital city of the state of the amazonas, where all the four denv serotypes were co-circulating simultaneously. | 2014 | 24978469 |
| new records of mosquitoes (diptera: culicidae) from bolívar state in south eastern venezuela, with 27 new species for the state and 5 of them new in the country. | this is the first part of a series of studies related to mosquito ecological and biogeographic aspects. a total of 69 mosquito species (diptera: culicidae) was collected in 16 localities sampled in the gran sabana municipality, canaima national park, and venezuela. twenty-seven mosquito species are recorded for the first time from bolívar state, venezuela. five of them species are reported for the first time in venezuela: anopheles malefactor dyar and knab (1907); chagasia bonneae root (1927); c ... | 2015 | 25853113 |
| mayaro virus infection alters glucose metabolism in cultured cells through activation of the enzyme 6-phosphofructo 1-kinase. | although it is well established that cellular transformation with tumor virus leads to changes on glucose metabolism, the effects of cell infection by non-transforming virus are far to be completely elucidated. in this study, we report the first evidence that cultured vero cells infected with the alphavirus mayaro show several alterations on glucose metabolism. infected cells presented a two fold increase on glucose consumption, accompanied by an increment in lactate production. this increase in ... | 2004 | 15646042 |
| [exanthematous diseases and the first epidemic of dengue to occur in manaus, amazonas state, brazil, during 1998-1999]. | in 1998, the fmt/imt-am foundation implemented the surveillance system to diagnose acute undifferentiated febrile syndromes, with the objective of active and passive surveillance in brazilian western amazonian rainforest to identify and diagnose the etiologic agents of acute fever. the diagnoses were performed using serological tests to detect igm antibodies by elisa (enzyme-linked-immunosorbent assay) cdc/opas or using commercial kits. a total of 8,557 serum samples obtained from patients with ... | 2004 | 15765597 |
| recurrent arthralgias in a patient with previous mayaro fever infection. | mayaro fever is an acute, self-limited, febrile, mosquito-borne viral disease manifested by fever, chills, headache, myalgias, and arthralgias. the virus belongs to the family togaviridae and the genus alphavirus. five other mosquito-borne viruses have been described as causing a similar dengue-like illness. the virus was first isolated in 1954, and the first epidemics were described in 1955 in brazil and bolivia. other cases have been reported in suriname, brazil, peru, french guiana, and trini ... | 2005 | 15898531 |
| evolutionary and ecological characterization of mayaro virus strains isolated during an outbreak, venezuela, 2010. | in 2010, an outbreak of febrile illness with arthralgic manifestations was detected at la estación village, portuguesa state, venezuela. the etiologic agent was determined to be mayaro virus (mayv), a reemerging south american alphavirus. a total of 77 cases was reported and 19 were confirmed as seropositive. mayv was isolated from acute-phase serum samples from 6 symptomatic patients. we sequenced 27 complete genomes representing the full spectrum of mayv genetic diversity, which facilitated de ... | 0 | 26401714 |
| isolation and characterization of mayaro virus from a human in acre, brazil. | mayaro virus (mayv) is widely distributed throughout south america and is the etiologic agent of mayaro fever, an acute febrile illness often presenting with arthralgic manifestations. the true incidence of mayv infection is likely grossly underestimated because the symptomatic presentation is very similar to that of dengue fever and other acute febrile tropical diseases. we report the complete genome sequence of a mayv isolate detected from an acrelândia patient presenting with fever, chills, a ... | 2014 | 25510721 |
| utilization of an eilat virus-based chimera for serological detection of chikungunya infection. | in december of 2013, chikungunya virus (chikv), an alphavirus in the family togaviridae, was introduced to the island of saint martin in the caribbean, resulting in the first autochthonous cases reported in the americas. as of january 2015, local and imported chikv has been reported in 50 american countries with over 1.1 million suspected cases. chikv causes a severe arthralgic disease for which there are no approved vaccines or therapeutics. furthermore, the lack of a commercially available, se ... | 2015 | 26492074 |
| emergence and potential for spread of chikungunya virus in brazil. | in december 2013, an outbreak of chikungunya virus (chikv) caused by the asian genotype was notified in the caribbean. the outbreak has since spread to 38 regions in the americas. by september 2014, the first autochthonous chikv infections were confirmed in oiapoque, north brazil, and in feira de santana, northeast brazil. | 2015 | 25976325 |
| mayaro virus: complete nucleotide sequence and phylogenetic relationships with other alphaviruses. | mayaro (may) virus is a member of the genus alphavirus in the family togaviridae. alphaviruses are distributed throughout the world and cause a wide range of diseases in humans and animals. here, we determined the complete nucleotide sequence of may from a viral strain isolated from a french guianese patient. the deduced may genome was 11,429 nucleotides in length, excluding the 5' cap nucleotide and 3' poly(a) tail. nucleotide and amino acid homologies, as well as phylogenetic analyses of the o ... | 2006 | 16343676 |
| pyruvate dehydrogenase kinase regulates hepatitis c virus replication. | during replication, hepatitis c virus (hcv) utilizes macromolecules produced by its host cell. this process requires host cellular metabolic reprogramming to favor elevated levels of aerobic glycolysis. therefore, we evaluated whether pyruvate dehydrogenase kinase (pdk), a mitochondrial enzyme that promotes aerobic glycolysis, can regulate hcv replication. levels of c-myc, hypoxia-inducible factor-1α (hif-1α), pdk1, pdk3, glucokinase, and serine biosynthetic enzymes were compared between hcv-inf ... | 2016 | 27471054 |
| extended preclinical safety, efficacy and stability testing of a live-attenuated chikungunya vaccine candidate. | we recently described a new, live-attenuated vaccine candidate for chikungunya (chik) fever, chikv/ires. this vaccine was shown to be well attenuated, immunogenic and efficacious in protecting against chik virus (chikv) challenge of mice and nonhuman primates. to further evaluate its preclinical safety, we compared chikv/ires distribution and viral loads in interferon-α/β receptor-incompetent a129 mice to another chik vaccine candidate, 181/clone25, which proved highly immunogenic but mildly rea ... | 2015 | 26340754 |
| proteomics profiling of chikungunya-infected aedes albopictus c6/36 cells reveal important mosquito cell factors in virus replication. | chikungunya virus (chikv) is the only causative agent of chikv fever with persistent arthralgia, and in some cases may lead to neurological complications which can be highly fatal, therefore it poses severe health issues in many parts of the world. chikv transmission can be mediated via the aedes albopictus mosquito; however, very little is currently known about the involvement of mosquito cellular factors during chikv-infection within the mosquito cells. unravelling the neglected aspects of mos ... | 2015 | 25738640 |
| false-negative dengue cases. | | 0 | 25923902 |
| anti-mayaro virus activity of cassia australis extracts (fabaceae, leguminosae). | the arthropod-borne mayaro virus (mayv) causes 'mayaro fever', a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical south america. studies showed that the virus could also be transmitted by the mosquito aedes aegypti. recently, mayv has attracted attention due to its likely urbanization. to date, there are no drugs that can treat this illness. | 2014 | 25428163 |
| chikungunya virus transmission between aedes albopictus and laboratory mice. | chikungunya virus (chikv) is a mosquito-borne alphavirus associated with epidemics of acute and chronic arthritic disease in humans. aedes albopictus has emerged as an important new natural vector for chikv transmission; however, mouse models for studying transmission have not been developed. | 2016 | 27760560 |
| sustained elevated cytokine levels during recovery phase of mayaro virus infection. | | 0 | 26981875 |
| chikungunya: epidemiology. | chikungunya virus is a mosquito-borne alphavirus that causes fever and debilitating joint pains in humans. joint pains may last months or years. it is vectored primarily by the tropical and sub-tropical mosquito, aedes aegypti, but is also found to be transmitted by aedes albopictus, a mosquito species that can also be found in more temperate climates. in recent years, the virus has risen from relative obscurity to become a global public health menace affecting millions of persons throughout the ... | 2016 | 26918158 |
| molecular detection of mayaro virus during a dengue outbreak in the state of mato grosso, central-west brazil. | mayaro virus (mayv) is frequently reported in pan-amazonia. the aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of mato grosso, brazil. serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. mayv was detected in 15 (2.5%) of 604 patients. twelve were co-infected with den ... | 2014 | 25141284 |
| hierarchical cluster analysis of three-dimensional reconstructions of unbiased sampled microglia shows not continuous morphological changes from stage 1 to 2 after multiple dengue infections in callithrix penicillata. | it is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response to specific pathogens. in the present report we quantitated microglia morphological changes in a monkey model of dengue disease with virus cns invasion. to mimic multiple infections that usually occur in endemic areas, where higher dengue infection incidence and abundant mosquito vectors carrying different serotypes coexist, subjects receiv ... | 2016 | 27047345 |
| diagnosis of arboviruses using indirect sandwich igg elisa in horses from the brazilian amazon. | the amazon as a whole is the largest reservoir of arboviruses worldwide, while the brazilian amazon hosts the largest variety of arboviruses isolated to date. in this study, the results of an indirect sandwich igg elisa, standardized for 19 arbovirustypes circulating among horses in brazilian amazon, were compared to results of the hemagglutination inhibition test. a screening test assessed the conditional probability distribution and a pearson linear correlation test determined the correlation ... | 2014 | 25013410 |
| vp4 protein from human rhinovirus 14 is released by pressure and locked in the capsid by the antiviral compound win. | rhinoviruses are the major causative agents of the common cold in humans. here, we studied the stability of human rhinovirus type 14 (hrv14) under conditions of high hydrostatic pressure, low temperature, and urea in the absence and presence of an antiviral drug. capsid dissociation and changes in the protein conformation were monitored by fluorescence spectroscopy, light scattering, circular dichroism, gel filtration chromatography, mass spectrometry and infectivity assays. the data show that h ... | 2007 | 17161425 |
| dichloroacetate blocks aerobic glycolytic adaptation to attenuated measles virus and promotes viral replication leading to enhanced oncolysis in glioblastoma. | targeting reprogrammed energy metabolism such as aerobic glycolysis is a potential strategy for cancer treatment. however, tumors exhibiting low-rate glycolysis or metabolic heterogeneity might be resistant to such treatment. we hypothesized that a therapeutic modality that drove cancer cells to high-rate glycolysis might sensitize cancer cells to interference directed against metabolic flux. in this study, we found that attenuated oncolytic measles virus edmonston strain (mv-edm) caused gliobla ... | 0 | 25575816 |
| hepatitis a and e seroprevalence and associated risk factors: a community-based cross-sectional survey in rural amazonia. | hepatitis a virus (hav) and hepatitis e virus (hev) are both transmitted by the faecal-oral route, and represent common causes of acute hepatitis in developing countries. the endemicity of hav infection has shifted from high to moderate in brazil. human cases of hev infection seem to be rare, although the virus has been detected in swine livestock and effluents of slaughterhouses. this study was to determine the epidemiology of hepatitis a and e in one of the largest agricultural settlements in ... | 2014 | 25149658 |
| a novel live-attenuated vaccine candidate for mayaro fever. | mayaro virus (mayv) is an emerging, mosquito-borne alphavirus that causes a dengue-like illness in many regions of south america, and which has the potential to urbanize. because no specific treatment or vaccine is available for mayv infection, we capitalized on an ires-based approach to develop a live-attenuated mayv vaccine candidate. testing in infant, immunocompetent as well as interferon receptor-deficient mice demonstrated a high degree of attenuation, strong induction of neutralizing anti ... | 2014 | 25101995 |
| arthritogenic alphaviruses--an overview. | mosquito-transmitted alphaviruses causing human rheumatic disease are globally distributed and include chikungunya virus, ross river virus, barmah forest virus, sindbis virus, o'nyong-nyong virus and mayaro virus. these viruses cause endemic disease and, occasionally, large epidemics; for instance, the 2004-2011 chikungunya epidemic resulted in 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. the disease is usually self-limiting and characterized by acute and chronic s ... | 2012 | 22565316 |
| the molecular and cellular aspects of arthritis due to alphavirus infections: lesson learned from ross river virus. | alphaviruses such as the sindbis-group viruses, scandinavian ockelbo virus, the african asian chikungunya virus, the african o'nyong-nyong virus, the south american mayaro virus, and the australasian barmah forest and ross river viruses, are commonly associated with outbreaks of acute and persistent arthritis and arthralgia in humans. the mechanisms by which these viruses cause arthritis/arthralgia are poorly understood. this chapter summarizes our current understanding of viral arthritides usin ... | 2007 | 17470914 |
| infection by una virus (alphavirus; togaviridae) and risk factor analysis in black howler monkeys (alouatta caraya) from paraguay and argentina. | a neutralizing antibody (ntab) survey on una and mayaro viruses in black howler monkeys (aloutta caraya) from subtropical regions of argentina and paraguay was carried out. risk factors for infection in monkeys were analyzed. no positive sera for mayaro virus were detected. a prevalence of 73% (61/84) of ntab against unav was detected with titers ranging from 20 to 1280. according to the statistical analysis performed, the monkey's age was a significant risk factor, but not the origin or sex. th ... | 2007 | 17658571 |
| lactate favours the dissociation of skeletal muscle 6-phosphofructo-1-kinase tetramers down-regulating the enzyme and muscle glycolysis. | for a long period lactate was considered as a dead-end product of glycolysis in many cells and its accumulation correlated with acidosis and cellular and tissue damage. at present, the role of lactate in several physiological processes has been investigated based on its properties as an energy source, a signalling molecule and as essential for tissue repair. it is noteworthy that lactate accumulation alters glycolytic flux independently from medium acidification, thereby this compound can regula ... | 2007 | 17666012 |
| mayaro virus: imported cases of human infection in são paulo state, brazil. | mayaro virus (mayv) is an arbovirus (togaviridae: alphavirus) enzootic in tropical south america and maintained in a sylvan cycle involving wild vertebrates and haemagogus mosquitoes. mayv cases occur sporadically in persons with a history of recent activities inside or around forests. this paper reports three cases of mayv fever detected in men infected in camapuã, ms, brazil. serum samples collected at four days and two months after the onset of the symptoms and examined by hemagglutination in ... | 2007 | 17823750 |
| evolutionary patterns of eastern equine encephalitis virus in north versus south america suggest ecological differences and taxonomic revision. | the eastern equine encephalitis (eee) complex consists of four distinct genetic lineages: one that circulates in north america (na eeev) and the caribbean and three that circulate in central and south america (sa eeev). differences in their geographic, pathogenic, and epidemiologic profiles prompted evaluation of their genetic diversity and evolutionary histories. the structural polyprotein open reading frames of all available sa eeev and recent na eeev isolates were sequenced and used in evolut ... | 2010 | 19889755 |
| a nonhuman primate model of chikungunya disease. | chikungunya disease is a severely debilitating, mosquito-borne, viral illness that has reached epidemic proportions in africa, asia, and the islands of the indian ocean. a mutation enhancing the ability of the chikungunya virus (chikv) to infect and be transmitted by aedes albopictus has increased the geographical range at risk for infection due to the continuing global spread of this mosquito. research into disease pathogenesis, vaccine development, and therapeutic design has been hindered by t ... | 2010 | 20179348 |
| chikungunya disease in nonhuman primates involves long-term viral persistence in macrophages. | chikungunya virus (chikv) is a mosquito-borne alphavirus that induces in humans a disease characterized by fever, rash, and pain in muscles and joints. the recent emergence or reemergence of chikv in the indian ocean islands and india has stressed the need to better understand the pathogenesis of this disease. previous chikv disease models have used young or immunodeficient mice, but these do not recapitulate human disease patterns and are unsuitable for testing immune-based therapies. herein, w ... | 2010 | 20179353 |
| a case of mayaro virus infection imported from french guiana. | emergence of arboviruses is a rising problem in several areas in the world. here we report a case of mayaro virus infection that was diagnosed in a french citizen presenting a dengue-like syndrome with prolonged arthralgia following a travel in french guiana. diagnosis was based on serological testing, a newly developed specific rt-pcr and sequencing. the real incidence of this viral infection among travelers is poorly known but this case is the first reported in a european area where aedes albo ... | 2016 | 26921736 |
| characterization of mitochondrial genome of haemagogus janthinomys (diptera: culicidae). | haemagogus janthinomys is a mosquito of high importance in public health due its involvement on natural wild cycles of two important arboviruses in the brazilian amazon region: yellow fever virus (flaviviridae, flavivirus) and mayaro virus (togaviridae, alphavirus). here, we have sequenced and described all the mitochondrial genes for the hg. janthinomys species. the complete coding sequence is14 937 bp long and includes 37 functional genes, of which 13 codes for proteins, 22 for trna and 2 for ... | 2017 | 26709451 |
| assessing yellow fever risk in the ecuadorian amazon. | this study reports results of a cross-sectional study based on interviews and seroepidemiological methods to identify risk factors for yellow fever infection among personnel of a military garrison in the amazonian rainforest. clinical symptoms and signs observed among yellow fever cases are also described. humoral immune response to yellow fever, mayaro, venezuelan equine encephalitis, oropouche, and dengue 2 infection was assessed by evaluating igm and igg specific antibodies. a yellow fever at ... | 2009 | 20300380 |
| global mapping of infectious disease. | the primary aim of this review was to evaluate the state of knowledge of the geographical distribution of all infectious diseases of clinical significance to humans. a systematic review was conducted to enumerate cartographic progress, with respect to the data available for mapping and the methods currently applied. the results helped define the minimum information requirements for mapping infectious disease occurrence, and a quantitative framework for assessing the mapping opportunities for all ... | 2013 | 23382431 |
| serosurvey of selected arboviral pathogens in free-ranging, two-toed sloths (choloepus hoffmanni) and three-toed sloths (bradypus variegatus) in costa rica, 2005-07. | we screened for antibodies to 16 arboviruses in four populations of free-ranging sloths in costa rica. blood samples were taken from 16 hoffman's two-toed sloths (htss; choloepus hoffmanni ) and 26 brown-throated sloths (btss; bradypus variegatus ) over a 3-yr period. we used serologic assays to detect antibodies against 10 arboviruses previously described in sloths (st. louis encephalitis [slev], changuinola, venezuelan equine encephalitis, ilheus [ilhv], oropouche, mayaro, utinga, murutucu, pu ... | 2016 | 27479900 |
| inhibition of mayaro virus infection by bovine lactoferrin. | mayaro virus (mayv) is an arbovirus linked to several sporadic outbreaks of a highly debilitating febrile illness in many regions of south america. mayv is on the verge of urbanization from the amazon region and no effective antiviral intervention is available against human infections. our aim was to investigate whether bovine lactoferrin (blf), an iron-binding glycoprotein, could hinder mayv infection. we show that blf promotes a strong inhibition of virus infection with no cytotoxic effects. m ... | 2014 | 24606707 |
| detection of arboviruses of public health interest in free-living new world primates (sapajus spp.; alouatta caraya) captured in mato grosso do sul, brazil. | a sero-epidemiological survey was undertaken to detect the circulation of arboviruses in free-living non-human primates. | 2014 | 24474008 |
| [mayaro: a re-emerging arbovirus in venezuela and latin america]. | mayaro virus produces nonspecific, sublethal disease symptoms, often confused with dengue, but with symptoms of arthalgias that can cause incapacitating disability. outbreaks have been localized and sporadic in the pan-amazonia forest since its first isolation in 1954 (trinidad and tobago). the literature available is scarce, diverse and dispersed. mayaro virus is an alphavirus, phylogenetically related to the semliki forest antigenic complex. una and mayaro viruses are the only viruses of this ... | 2012 | 23242303 |
| divergent cerebrospinal fluid cytokine network induced by non-viral and different viral infections on the central nervous system. | meningoencephalitis is one of the most common disorders of the central nervous system (cns) worldwide. viral meningoencephalitis differs from bacterial meningitis in several aspects. in some developing countries, bacterial meningitis has appropriate clinical management and chemotherapy is available. virus-associated and virus not detected meningoencephalitis are treatable, however, they may cause death in a few cases. the knowledge of how mediators of inflammation can induce disease would contri ... | 2015 | 26286516 |
| will mayaro virus be responsible for the next outbreak of an arthropod-borne virus in brazil? | mayaro virus (mayv) is an alphavirus from the togaviridae family and is transmitted mainly by hemagogus mosquitoes. this virus circulates in high-density tropical forests or rural areas of central and south america causing a disease characterized by high-grade fever, maculopapular skin rash and marked arthralgia that, in some patients, can persist for long periods after infection and may be misinterpreted as chikungunya. although only a few outbreaks involving this virus have been reported, in t ... | 2017 | 28688628 |
| neutralising antibodies for mayaro virus in pantanal, brazil. | the pantanal hosts diverse wildlife species and therefore is a hotspot for arbovirus studies in south america. a serosurvey for mayaro virus (mayv), eastern (eeev), western (weev) and venezuelan (veev) equine encephalitis viruses was conducted with 237 sheep, 87 free-ranging caimans and 748 equids, including 37 collected from a ranch where a neurologic disorder outbreak had been recently reported. sera were tested for specific viral antibodies using plaque-reduction neutralisation test. from a t ... | 2015 | 25742272 |
| performance of the tourniquet test for diagnosing dengue in peru. | the tourniquet test (tt) is a physical examination maneuver often performed on patients suspected of having dengue. it has been incorporated into dengue diagnostic guidelines and is used in clinical studies. however, little is known about tt performance characteristics in different patient types or epidemiologic conditions. in the dengue-endemic city of iquitos, peru, we performed tts and dengue laboratory assays on 13,548 persons with febrile disease, recruited through either active (n = 1,095) ... | 2013 | 23716410 |
| dengue research: a bibliometric analysis of worldwide and arab publications during 1872-2015. | dengue is an important emerging and re-emerging arboviral infection globally as a rapidly growing and widespread public health problem, with transmission occurring in more than 128 countries in asia, americas, southeast africa, western pacific, and eastern mediterranean regions. therefore, the aim of this study was to characterize and quantify the scientific output of dengue research in arab countries relative to that worldwide by using a bibliometric analysis. | 2016 | 27154247 |