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malaria in south america: a drug discovery perspective.the challenge of controlling and eventually eradicating malaria means that new tools are urgently needed. south america's role in this fight spans both ends of the research and development spectrum: both as a continent capable of discovering and developing new medicines, and also as a continent with significant numbers of malaria patients. this article reviews the contribution of groups in the south american continent to the research and development of new medicines over the last decade. therefo ...201323706107
seasonal distribution of malaria vectors (diptera: culicidae) in rural localities of porto velho, rondônia, brazilian amazon.we conducted a survey of the malaria vectors in an area where a power line had been constructed, between the municipalities of porto velho and rio branco, in the states of rondônia and acre, respectively. the present paper relates to the results of the survey of anopheles fauna conducted in the state of rondônia. mosquito field collections were performed in six villages along the federal highway br 364 in the municipality of porto velho, namely porto velho, jaci paraná, mutum paraná, vila abunã, ...026200969
malaria vectors in san josé del guaviare, orinoquia, colombia.this study was conducted to determine anopheles species composition and their natural infectivity by human plasmodium in 2 localities with the highest malaria transmission in san jose del guaviare, guaviare, colombia. a total of 1,009 anopheles mosquitoes were collected using human landing catches during 8 months in 2010. anopheles darlingi was the most abundant (83.2%) followed by an. albitarsis s.l. (8.6%), anopheles braziliensis (3.8%), an. oswaldoi s.l. (1%), and an. rangeli (0.3%). anophele ...025102591
[anopheles (díptera: culicidae) vectors of malaria in puerto carreño municipality, vichada, colombia].the study of the biological aspects of anopheles spp., strengthens the entomological surveillance.023235809
molecular phylogeny of neotropical anopheles (nyssorhynchus) albitarsis species complex (diptera: culicidae).a phylogeny was reconstructed for four species belonging to the neotropical anopheles (nyssorhynchus) albitarsis complex using partial sequences from the mitochondrial cytochrome oxidase i (coi) and nadh dehydrogenase 4 (nd4) genes and the ribosomal dna its2 and d2 expansion region of the 28s subunit. the basis for initial characterization of each member of the complex was by correlated random amplification of polymorphic dna-polymerase chain reaction (rapd-pcr) markers. analyses were carried ou ...018079976
adaptation of a south american malaria vector to laboratory colonization suggests faster-male evolution for mating ability.anopheles (nyssorhynchus) albitarsis (diptera: culicidae) is one of the very few south american mosquito vectors of malaria successfully colonized in the laboratory. these vectors are very hard to breed because they rarely mate in artificial conditions. a few years ago a free-mating laboratory colony of an. albitarsis sensu stricto was established after about 30 generations of artificial-mating. to begin to understand the process of adaptation of these malaria vectors to the laboratory we have c ...200415132759
malaria vectors in south america: current and future scenarios.malaria remains a significant public health issue in south america. future climate change may influence the distribution of the disease, which is dependent on the distribution of those anopheles mosquitoes competent to transmit plasmodium falciparum. herein, predictive niche models of the habitat suitability for p. falciparum, the current primary vector anopheles darlingi and nine other known and/or potential vector species of the neotropical albitarsis complex, were used to document the current ...201526283539
a nuclear ribosomal dna pseudogene in triatomines opens a new research field of fundamental and applied implications in chagas disease.a pseudogene, designated as "ps(5.8s+its-2)", paralogous to the 5.8s gene and internal transcribed spacer (its)-2 of the nuclear ribosomal dna (rdna), has been recently found in many triatomine species distributed throughout north america, central america and northern south america. among characteristics used as criteria for pseudogene verification, secondary structures and free energy are highlighted, showing a lower fit between minimum free energy, partition function and centroid structures, a ...201525760450
the modulation of the symbiont/host interaction between wolbachia pipientis and aedes fluviatilis embryos by glycogen metabolism.wolbachia pipientis, a maternally transmitted bacterium that colonizes arthropods, may affect the general aspects of insect physiology, particularly reproduction. wolbachia is a natural endosymbiont of aedes fluviatilis, whose effects in embryogenesis and reproduction have not been addressed so far. in this context, we investigated the correlation between glucose metabolism and morphological alterations during a. fluviatilis embryo development in wolbachia-positive (w+) and wolbachia-negative (w ...201424926801
geographic distribution, evolution, and disease importance of species within the neotropical anopheles albitarsis group (diptera, culicidae).the anopheles albitarsis group of mosquitoes comprises eight recognized species and one mitochondrial lineage. our knowledge of malaria vectorial importance and the distribution and evolution of these taxa is incomplete. we constructed ecological niche models (enms) for these taxa and used hypothesized phylogenetic relationships and enms to investigate environmental and ecological divergence associated with speciation events. two major clades were identified, one north (clade 1) and one south (c ...024820570
dna barcoding reveals both known and novel taxa in the albitarsis group (anopheles: nyssorhynchus) of neotropical malaria vectors.mosquitoes belonging to the albitarsis group (anopheles: nyssorhynchus) are of importance as malaria vectors across the neotropics. the group currently comprises six known species, and recent studies have indicated further hidden biodiversity within the group. dna barcoding has been proposed as a highly useful tool for species recognition, although its discriminatory utility has not been verified in closely related taxa across a wide geographic distribution.201222353437
germ band retraction as a landmark in glucose metabolism during aedes aegypti embryogenesis.the mosquito a. aegypti is vector of dengue and other viruses. new methods of vector control are needed and can be achieved by a better understanding of the life cycle of this insect. embryogenesis is a part of a. aegypty life cycle that is poorly understood. in insects in general and in mosquitoes in particular energetic metabolism is well studied during oogenesis, when the oocyte exhibits fast growth, accumulating carbohydrates, lipids and proteins that will meet the regulatory and metabolic n ...201020184739
classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of chaco, argentina.to classify mosquito species based on common features of their habitats, samples were obtained fortnightly between june 2001-october 2003 in the subtropical province of chaco, argentina. data on the type of larval habitat, nature of the habitat (artificial or natural), size, depth, location related to sunlight, distance to the neighbouring houses, type of substrate, organic material, vegetation and algae type and their presence were collected. data on the permanence, temperature, ph, turbidity, ...201121739026
egg maturation in neotropical malaria vectors: one blood meal is usually enough.the requirement of more than one blood meal to complete a gonotrophic cycle is reported here to be infrequent among field-collected anopheles (nyssorhynchus), including anopheles darlingi, south america's most important malaria vector. the overall frequencies of host-seeking females that did not develop eggs after one blood feeding were low for anopheles darlingi (6.6%), anopheles rondoni (5.0%), and anopheles triannulatus (2.2%); the majority of wild-caught females that did not develop eggs aft ...19989879075
an evaluation of the suitability of coi and coii gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (diptera culicidae).we assessed the practicality and effectiveness of using variation in the mitochondrial coi and coii genes to discriminate species and reconstruct the phylogeny of anophelene mosquitoes. phylogenetic relationships among the subfamily anophelinae were inferred from portions of the mitochondrial coi (92 species) and coii genes (108 species). phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood and bayesian methods. the suitability of coi and coii gene variation for id ...201627937065
the phylogenetic relationships of known mosquito (diptera: culicidae) mitogenomes.the known mosquito mitogenomes, containing a total of 34 species, which belong to five genera, were collected from genbank, and the practicality and effectiveness of the variation in the complete mitochondrial dna genome and portions of mitochondrial coi gene were assessed to reconstruct the phylogeny of mosquitoes. phylogenetic trees were reconstructed on the basis of parsimony, maximum likelihood, and bayesian (bi) methods. it is concluded that: (1) both mitogenomes and coi gene support the mo ...201627731651
mitochondrial genomes of anopheles (kerteszia) (diptera: culicidae) from the atlantic forest, brazil.mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, such as the anopheles albitarsis complex. except for a few studies that employed a limited number of nuclear or mitochondrial loci to address the genetic structure and species status of anopheles cruzii, anopheles bellator, and anopheles homunculus, little is known about genetic markers that can be employed in studies focusing on kerteszia species. the complete mitochondria ...201627146682
first record of anopheles oryzalimnetes, anopheles argyritarsis, and anopheles sawyeri (diptera: culicidae) in the caatinga biome, semiarid scrubland of sergipe state, brazil.caatinga is one of the least known biomes of brazil in relation to biodiversity. the dry condition of semiarid areas has been associated in the past with low richness of fauna and flora, not encouraging studies in this region. there is a lack of mosquito records including anophelines. thus, to investigate the biodiversity of anopheles mosquitoes in the caatinga biome, we collected immature mosquitoes in aquatic habitats in a conservation reserve located in the northwestern portion of sergipe sta ...201526336257
the influence of the area of the serra da mesa hydroelectric plant, state of goiás, on the frequency and diversity of anophelines (diptera: culicidae): a study on the effect of a reservoir.bioecological aspects of anophelines (diptera: culicidae) near areas under the direct influence of the hydroelectric plant reservoir of serra da mesa in goiás, brazil, were analyzed.201525860461
on the use of classic epidemiological formulae for estimating the intensity of endemic malaria transmission by vectors in the amazon.although various reports have described entomological inoculation rates of malaria vector species, most were limited to providing descriptive field data. here, we report biting rates and survival data for two important malaria vectors in the amazon, anopheles darlingi (root) and anopheles albitarsis e (lynch-arribalzaga) (diptera: culicidae), in the state of roraima, brazil. we calculated theoretical sporozoite infection rates and critical vector biting rates for these species during 1 year, com ...201223950095
wing geometric morphometrics and molecular assessment of members in the albitarsis complex from colombia.malaria parasites are transmitted to humans by female mosquitoes of the genus anopheles. the albitarsis complex harbours at least eight species not readily differentiable by morphology. this complicates the determination of those species involved in malaria transmission and the implementation of targeted and effective vector control strategies. in colombia, there is little information about the identity and distribution of the albitarsis complex members. in this work, coi dna barcoding was used ...201323702155
host range of the parasite strelkovimermis spiculatus (nematoda: mermithidae) in argentina mosquitoes.strelkovimermis spiculatus (poinar and camino 1986) is a nematode parasite of mosquitoes isolated from the neotropical region. we investigated the host range of this parasite in mosquito populations for a better understanding of the dynamics of temporary breeding sites. five grassy-pool habitats filled by rainwater were sampled from the summer 2007 through the fall 2008. eight mosquito species were collected: anopheles albitarsis, culex chidesteri, culex dolosus, culex maxi, aedes albifasciatus, ...201323701609
ecology of anopheline mosquitoes (diptera: culicidae) in the central atlantic forest biodiversity corridor, southeastern brazil.knowledge of the fauna composition of anopheline mosquitoes, their ecological aspects and behavior, and influence of climatic variables on their population dynamics can help in understanding the transmission of plasmodium parasites and thus develop more efficient strategies for the control of malaria. in the central atlantic forest biodiversity corridor, southeastern brazil, foci of introduced malaria have been reported among people returning from the amazon region, north brazil. our objective w ...201323427648
confirmation of the occurrence of anopheles (nyssorhynchus) marajoara in french guiana.throughout the early entomological campaigns in french guiana (1900-1945), the presence of members of the anopheles albitarsis complex was reported in many places across the territory. however, since then no specimen has been caught despite many entomological studies conducted on the littoral and along the main rivers in places where malaria was endemic. we report here the 1st catches in the modern period of specimens of the an. albitarsis complex in the deep rainforest. during a military interv ...201223393754
isolation and characterization of 25 microsatellite dna loci for anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.the anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in brazil. twenty-five polymorphic microsatellite dna loci were isolated and characterized in 24-36 individuals from the neighborhood of puraquequara, manaus, amazonas state, brazil. the number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. eleven loci showed significant deviation from hardy-wein ...201223315804
feeding patterns of mosquitoes (diptera: culicidae) in six brazilian environmental preservation areas.feeding patterns of mosquitoes in six brazilian environmental preservation areas were analyzed by the precipitin technique. the mosquito populations were captured using shannon traps during different time periods. bird, cow, dog, horse, opossum, human, and rodent antisera diagnostic tests were employed and results were analyzed by calculating the sørensen similarity index and using the null-model test. of the 647 analyzed specimens, 443 reacted to the utilized antisera, of which 331 reacted to o ...201223181858
analysis of reproductive isolation between sibling species anopheles albitarsis sensu stricto and anopheles deaneorum, two malaria vectors belonging to the albitarsis complex (diptera: culicidae).complexes of sibling species are common among mosquitoes, and their existence within vector species can have important epidemiological consequences. anopheles albitarsis sensu stricto and anopheles deaneorum rosa-freitas are two putative vectors of malaria parasites belonging to the albitarsis species complex (diptera: culicidae). using an induced mating technique, we studied the reproductive isolation between these two closely related species and their reciprocal hybrids. evidence for hybrid ma ...200415535617
temperature influence on embryonic development of anopheles albitarsis and anopheles aquasalis.temperature influence on the embryonic development of anopheles aquasalis and an. albitarsis was investigated. at 26 degrees c, 75% and 60% of respectively an. aquasalis and an. albitarsis eggs hatched, with one peak of eclosion, between the 2nd and 3rd day after oviposition. at 20 +/- 2 degrees c, around 66-70% of an. aquasalis eggs hatched, with one eclosion peak, on the 5th day. on the other hand, an. albitarsis eclosion at 21+/- 2 degrees c decreased to 10-22%, with two eclosion peaks, on th ...200212563476
anopheles albitarsis embryogenesis: morphological identification of major events.anopheles albitarsis embryogenesis was analyzed through confocal microscopy of clarified eggs. using drosophila melanogaster as reference system, the major morphogenetic events (blastoderm, gastrulation, germ band extension, germ band retraction, dorsal closure) were identified. the kinetics of early events is proportionally similar in both systems, but late movements (from germ band retraction on) progress slower in an. albitarsis. major differences in an. albitarsis related to d. melanogaster ...200212118297
[parity and ovarian development of anopheles albitarsis l.s.in irrigated agroecosystem field].to determine the parity, ovarian development and survival rates of anopheles albitarsis species, in order to estimate the potential of malaria transmission.200010769358
observations on the flight and longevity in nature of anopheles albitarsis domesticus. 195014795210
[information concerning the anopheles albitarsis domesticus, one of the transmitters of malaria in brazil (diptera, culicidae)]. 195015405237
composition of anopheline (diptera: culicidae) community and its seasonal variation in three environments of the city of puerto iguazú, misiones, argentina.in order to extend the knowledge of the composition of the anopheline community and the seasonal variation related to anthropogenic modifications in the city of puerto iguazú, adult females were captured between 2009 and 2012. samples were collected in three environments with different degrees of anthropogenic modification: urban, periurban, and wild. alpha diversity was evaluated as the 'true' diversity of the species in each environment. among environments, range-abundance curves were used to ...201729194513
characterization of the complete mitogenome of anopheles aquasalis, and phylogenetic divergences among anopheles from diverse geographic zones.whole mitogenome sequences (mtdna) have been exploited for insect ecology studies, using them as molecular markers to reconstruct phylogenies, or to infer phylogeographic relationships and gene flow. recent anopheles phylogenomic studies have provided information regarding the time of deep lineage divergences within the genus. here we report the complete 15,393 bp mtdna sequences of anopheles aquasalis, a neotropical human malaria vector. when comparing its structure and base composition with ot ...201931479460
species diversity and abundance of anopheles (nyssorhynchus) (diptera: culicidae) in cachoeiras de macacu municipality, rio de janeiro state: an area of the atlantic forest receptive and vulnerable to malaria.the present study evaluated the diversity of anopheles meigen, 1818 belonging to the subgenus nyssorhynchus blanchard, 1902 in four areas of cachoeiras de macacu municipality, rio de janeiro, brazil. the immature collections were carried out during a rainy period and a post-rainy season. during the two periods, 3,932 specimens of anopheles (nyssorhynchus) were collected: 562 in the rainy period (14.29%) and 3,370 in the post-rainy period (85.70%). among 3,932 specimens collected during both peri ...201930649408
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