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phenolic compounds of pinus laricio needles: a bioindicator of the effects of prescribed burning in function of season.fire is a dominant ecological factor in mediterranean-type ecosystems. forest management includes many preventive tools, in particular for fire prevention, such as mechanical treatments and prescribed burning. prescribed burning is a commonly used method for treating fuel loads, but fuel reduction targets for reducing wildfire hazards must be balanced against fuel retention targets in order to maintain habitat and other forest functions. this approach was used on pinus nigra ssp laricio var. cor ...200919446863
flame atomic absorption spectrometric determination of pb, cd, and cu in pinus nigra l. and eriobotrya japonica leaves used as biomonitors in environmental pollution.the assessment of trace metal pollution in gaziantep city-turkey has been studied using plant leaves of pinus nigra l. and eriobotrya japonica as biomonitor. the concentrations up to 3,056 mg pb kg(-1) in the needles of pinus nigra l., and 367 ng cd g(-1) in the leaves of eriobotrya japonica were determined. the observed cu concentrations were in range of 1.6-7.1 mg kg(-1). the pb, cd, and cu levels in soils were determined to be in the range of 17-602, 0.142-0.656, and 12-38 mg kg(-1), respecti ...201019784536
physical, chemical and pedogenetical properties of soil in relation with altitude at kazdagi upland black pine forest.in this study an attempt was made to investigate the physical, chemical and pedogenetical soil properties of black pine forests at over 1400 metre (m) altitude of kazdagi mountainous uplands. soil profiles (37 numbers) were dug, examined and sampled considering the horizons and soil volume weight, fine soil weight, stoniness, ph, total nitrogen, soil organic carbon and pedogenesis alterations were tried to be explored in relation with elevating altitude. while the altitude increased, soil organi ...200920120457
ectomycorrhizal fungal communities of native and non-native pinus and quercus species in a common garden of 35-year-old trees.non-native tree species have been widely planted or have become naturalized in most forested landscapes. it is not clear if native trees species collectively differ in ectomycorrhizal fungal (emf) diversity and communities from that of non-native tree species. alternatively, emf species community similarity may be more determined by host plant phylogeny than by whether the plant is native or non-native. we examined these unknowns by comparing two genera, native and non-native quercus robur and q ...201121573837
effect of thinning on density of pinus nigra tree using x-ray computed tomography.this study was carried out to determine the effect of thinning on density, extractives and lignin content of pinus nigra amold tree. wood density was measured with computed tomography and 540+/-20 kg m(-3) were determined on control samples. density of samples from thinning applied plot was 520+/-20 kg m(-3). the results showed that thinning slightly changes the density of wood and this change is caused by springwood content. in contrast, extractives and lignin content were not changed with thin ...200920120459
studies on antimicrobial activity of pyramidal black pine (pinus nigra ssp. pallasiana var. pyramidata): an endemic plant close to become extinct.turkey is one of the richest areas at middle latitudes in terms of plant diversity. one of turkey's (anatolia) endemic plants is pyramidal black pine (pinus nigra ssp. pallasiana var. pyramidata). but, it was observed that the pyramidal black pine, which is part of turkey's important biological richness, came close to become extinct. this study was carried out to investigate the drying of the pyramidal black pine in the specific vakifkoy area having semi arid to humid climatic conditions on the ...200920121018
plant ribonucleases and nucleases as antiproliferative agens targeting human tumors growing in mice.the antiproliferative and antitumor effect of leaf ribonuclease was tested in vitro on the human ml-2 tumor cell line and in vivo on athymic nude mice bearing human melanoma tumors. the antiproliferative activity of this plant ribonuclease in vitro studies was negligible. in the experiments in vivo a significant decrease of the tumor size, however was observed. from nucleases the mung bean nuclease (pha) was studied first from nucleases. the antitumor effect of this enzyme on ml2 human tumor cel ...201020218957
species-specific responses of pine sesquiterpene synthases to sawfly oviposition.pinus sylvestris (scots pine) is known to respond to eggs laid by the sawfly diprion pini on its needles by releasing a blend of terpenoids, including the sesquiterpene (e)-beta-farnesene. these compounds attract a wasp, closterocerus ruforum, which parasitizes sawfly eggs. d. pini oviposition also enhances the transcription of two sesquiterpene synthases, an (e)-beta-caryophyllene/alpha-humulene synthase (pstps1) and a 1(10),5-germacradiene-4-ol synthase (pstps2). to gain a better understanding ...201020394954
acetylcholinesterase and butyrylcholinesterase inhibitory activity of pinus species essential oils and their constituents.this study aimed to investigate the acetylcholinesterase (ache) and butyrylcholinesterase (bche) inhibitory activity of the essential oils from pinus nigra subsp. nigra, p. nigra var. calabrica, and p. heldreichii subsp. leucodermis. this activity is relevant to the treatment of alzheimer's disease (ad), since cholinesterase drugs are currently the only drugs available to treat ad. p. heldreichii subsp. leucodermis exhibited the most promising activity, with ic(50) values of 51.1 and 80.6 microg ...201020429778
cloning and expression of a toxin gene from pseudomonas fluorescens gcm5-1a.pseudomonas fluorescens gcm5-1a was isolated from the pine wood nematode (pwn), bursaphelenchus xylophilus, obtained from wilted japanese black pine, pinus thumbergii, in china. in this paper, a genomic library of the gcm5-1a strain was constructed and a toxin-producing clone was isolated by bioassay. nucleotide sequence analysis revealed an open reading frame of 1,290 bp encoding a protein of 429 amino acids with n-terminal putative signal peptide of 36 amino acids, which shared a similarity of ...201020495785
a fungal pathogen in time and space: the population dynamics of beauveria bassiana in a conifer forest.the fungal entomopathogen beauveria bassiana is ubiquitous in below-ground systems; however, there is a dearth of information on the above-ground diversity, temporal and spatial distribution of this fungus. therefore, we assessed its occurrence in a conifer forest (pseudotsuga monziesii and pinus nigra var. maritima) using selective media to isolate b. bassiana from soil, branch and bark samples collected in october 2005, march and june 2006. fungal density was the highest at all locations in oc ...201020662927
epipactis helleborine shows strong mycorrhizal preference towards ectomycorrhizal fungi with contrasting geographic distributions in japan.epipactis helleborine (l.) crantz, one of the most widespread orchid species, occurs in a broad range of habitats. this orchid is fully myco-heterotrophic in the germination stage and partially myco-heterotrophic in the adult stage, suggesting that a mycorrhizal partner is one of the key factors that determines whether e. helleborine successfully colonizes a specific environment. we focused on the coastal habitat of japanese e. helleborine and surveyed the mycorrhizal fungi from geographically d ...200818661158
use of magnetic resonance microscopy for the nondestructive observation of xylem cavitation caused by pine wilt disease.abstract the development of xylem cavitation caused by pine wilt disease was visualized nondestructively with a compact magnetic resonance (mr) microscope system. a t(1)-weighted spin-echo sequence clearly visualized the water-filled xylem of japanese black pine (pinus thunbergii) as white zones, whereas cavitated xylem was represented as dark areas. cavitated areas in the xylem were first observed 6 to 9 days after inoculation with the pinewood nematode (bursaphelenchus xylophilus), and enlarge ...200518943004
soil properties and root biomass responses to prescribed burning in young corsican pine (pinus nigra arn.) stands.fire is an important tool in the management of forest ecosystems. although both prescribed and wildland fires are common in turkey, few studies have addressed the influence of such disturbances on soil properties and root biomass dynamics. in this study, soil properties and root biomass responses to prescribed fire were investigated in 25-year-old corsican pine (pinus nigra arn.) stands in kastamonu, turkey. the stands were established by planting and were subjected to prescribed burning in july ...201021047013
the strait of gibraltar as a major biogeographic barrier in mediterranean conifers: a comparative phylogeographic survey.the strait of gibraltar (sg) is reputed for being both a bridge and a geographic barrier to biological exchanges between europe and africa. major genetic breaks associated with this strait have been identified in various taxa, but it is unknown whether these disjunctions have been produced simultaneously or by independent biogeographic processes. here, the genetic structure of five conifers distributed on both sides of the sg was investigated using mitochondrial (nad1 b/c, nad5-1, nad5-4 and nad ...201021073588
vegetation geography of western part of elmacik mountain, turkey.the study examines the western part of elmacik mountain from the perspective of vegetation geography. research area is within the phytogeographical region of euro-siberian that is among the flora and phytogeographical region of turkey. according to turkey's grid square system, the research area is located in the a3 square. the main elements of forest formation consist of fagus orientalis, abies nordmanniana subspp. bommuelleriana, pinus nigra subsp. pallasiana, pinus sylvestris, quercus spp., pl ...201223424832
different karyotype patterns among allopatric pinus nigra (pinaceae) populations revealed by molecular cytogenetics.to examine variation and taxonomic recognition of pinus nigra (european black pine) at the intraspecific level, chromosomal distribution of 5s and 18s-5.8s-26s rdna loci revealed by fluorescent in situ hybridisation (fish) and fluorochrome banding with chromomycin a(3) and dapi were analysed among allopatric populations belonging to different subspecies. despite prevalent opinion on predominantly conserved and homogenous conifer karyotypes, several patterns were observed. surprisingly, interstit ...201121143741
effect of defoliation by the pine processionary moth (ppm) on radial, height and volume growth of crimean pine (pinus nigra) trees in turkey.in this study, we assessed the effects of chronic defoliation on radial, height and volume growth of crimean pine (pinus nigra arnold) trees of the pine processionary moth [thaumetopoea wilkinsoni tams (lepidoptera:thaumetopoeidae)] in western turkey crimean pine tree ring chronologies were analyzed for evidence of the pine processionary moth (ppm). tree ring widths from non-defoliated crimean pine sample trees, which were not defoliated by ppm from 1998 to 2004, were used to estimate potential ...201021186719
differential response of two pinus spp. to avian nitrogen input as revealed by nitrogen isotope analysis for tree rings.temporal variations in n concentration and d(15)n value of annual tree rings (1 year of time resolution) of two japanese black pine (pinus thunbergii) and three japanese red pine (pinus densiflora) trees under current breeding activity of the great cormorant (pharacrocorax carbo) and the black-tailed gull (larus crassirostris), respectively, in central and northeastern japan were studied. both species from control sites where no avian input occurs show negative values (d(15)n = around -4 ‰ to -2 ...201121271423
carbohydrate catabolism in populations of bursaphelenchus xylophilus and in b. mucronatus.genotypically different host specific pathotypes of bursaphelenchus xylophilus have been identified. these pathotypes elicit different responses in pines depending on susceptibility, tolerance, or resistance. continued passage of some of these pathotypes on fungal cultures leads to conversion to nonparasitic populations. these populations metabolize carbon substrates to ethanol by an anaerobic pathway, while operating some level of a phosphoenolpyruvate (pep)-succinate pathway to excrete succina ...198819290209
genomic differences among pathotypes of bursaphelenchus xylophilus.total genomic dna from bursaphelenchus xylophilus pathotypes mpsy-1 and vpst-1 and from b. mucronatus was digested with restriction endonucleases. dna fragments were electrophoretically separated, southern blotted to nitrocellulose, and hybridized to genomic dna from one of the isolates. the resulting hybridization patterns indicate genomic differences in repetitive dna sequences among these populations. greatest differences were seen between b. xylophilus and b. mucronatus, but genomic differen ...198819290214
chemical composition and antimicrobial activity of the essential oil of endemic dalmatian black pine (pinus nigra ssp. dalmatica).the chemical composition and the antimicrobial activity of the essential oil isolated from the needles of endemic dalmatian black pine (pinus nigra ssp. dalmatica) from croatia were investigated. the chemical composition of the essential oil was determined by gc and gc/ms analyses, and the main compounds identified were a-pinene, ß-pinene, germacrene d, and ß-caryophyllene. disc-diffusion and broth-microdilution assays were used for the in vitro antimicrobial screening. the dalmatian black pine ...201121404437
back from a predicted climatic extinction of an island endemic: a future for the corsican nuthatch.the corsican nuthatch (sitta whiteheadi) is red-listed as vulnerable to extinction by the iucn because of its endemism, reduced population size, and recent decline. a further cause is the fragmentation and loss of its spatially-restricted favourite habitat, the corsican pine (pinus nigra laricio) forest. in this study, we aimed at estimating the potential impact of climate change on the distribution of the corsican nuthatch using species distribution models. because this species has a strong tro ...201121464916
distribution and sources of pahs using three pine species along the ebro river.needles of three pine species (pinus halepensis, pinus pinea and pinus nigra) were analysed to assess the occurrence of polycyclic aromatic hydrocarbons (pahs) in 34 sites located throughout the ebro river, in northeast spain. overall, the concentration varied between 55 and 808 ng g(?-?1) (dry weight). the three- and four-ring pahs were the most representative, with phenanthrene having 43% of the total pah load and naphthalene showing a high incidence in rural areas. despite matrix apparent sim ...201121465131
buffered climate change effects in a mediterranean pine species: range limit implications from a tree-ring study.within-range effects of climatic change on tree growth at the sub-regional scale remain poorly understood. the aim of this research was to use climate and radial-growth data to explain how long-term climatic trends affect tree growth patterns along the southern limit of the range of pinus nigra ssp. salzmannii (eastern baetic range, southern spain). we used regional temperature and precipitation data and measured sub-regional radial growth variation in p. nigra forests over the past two centurie ...201121562865
distribution of bacterial species in soil with a vegetational change from japanese black pine (pinus thunbergii) to black locust (robinia pseudoacacia).the effects of a nitrogen-fixing tree, black locust (robinia pseudoacacia), on the distribution of bacterial species were examined in a japanese black pine (pinus thunbergii) and black locust-dominated area. dna was extracted from the soil at depths of 0-5 and 5-10 cm, collected at the border between a japanese black pine-dominated forest and a black locust-dominated forest, and the distribution of bacterial species was investigated by denaturing gradient gel electrophoresis (dgge). the bacteria ...200921566380
climate change impacts and vulnerability of the southern populations of pinus nigra subsp. salzmannii.the understanding of regional vulnerability to climate change in mediterranean mountain forests is not well developed. climate change impacts on tree growth should be strongly related to the steep environmental gradients of mountainous areas, where a temperature-induced upward shift of the lower elevation limit is expected, particularly amongst drought-sensitive species. trees will adapt not only to changes in mean climate variables but also to increased extreme events such as prolonged drought. ...201020522420
the melliferous potential of forest and meadow plant communities on mount tara (serbia).the apiflora of 34 forest and meadow plant communities in tara national park was studied with the aim of assessing their melliferous potential and their contribution to bee pasture during the vegetation period. the melliferous plants were analyzed individually from the aspect of their flowering phenology, abundance, and the intensity of nectar and pollen production, as well as the production of honeydew. the melliferous potential of each investigated plant community was theoretically assessed on ...201323905735
quantification of hydrogen peroxide in plant tissues using amplex red.reactive oxygen species (ros) are by-products of photosynthesis and respiration in plant tissues. abiotic and biotic stressors also induce the production and temporary accumulation of ros in plants, including hydrogen peroxide (h2o2), whereby they can act as secondary messengers/chemical mediators in plant defense signaling and lead to programmed cell death. h2o2 acts as a hub for critical information flow in plants. despite such key roles in fundamental cellular processes, reliable determinatio ...201627476009
predicting the above-ground biomass of crimean pine (pinus nigra) stands in turkey.in this study, biomass equations are presented for crimean pine (pinus nigra arnold) stands within the zonguldak forest region. a total of 44 sample plots, each of 0.04 ha were chosen in order to define the biomass equations of black pine, the most common needle leaved species in turkey. a tree which is the most similar to mean free according to basal area was cut in each sample area as a sample tree. various models were tested, utilizing the diameter (d) and the height (h) as independent variab ...201020648821
anatomical basis for biophysical differences between pinus nigra and p. resinosa (pinaceae) leaves.differences in the flexibility of pinus nigra and p. resinosa leaves can be used to discriminate these two similarly looking pine species from one another. when bent along the longitudinal axis, p. resinosa leaves snap, while p. nigra leaves appear flexible. this useful field test has had no known biophysical or anatomical explanation until now. analysis of the first order mechanics of bending and buckling of the pine needles was used to elucidate any important anatomical differences between the ...200821632324
high-resolution melting analysis: a new molecular approach for the early detection of diplodia pinea in austrian pine.the differentiation of diplodia pinea from closely related species, such as diplodia scrobiculata and diplodia seriata, and its detection in plant tissue, represented a critical issue for a long time. molecular screening tools have recently been developed to address this topic. in this study we applied one of the most sensitive and rapid diagnostic screening method so far developed, called high-resolution melting analysis (hrma), to detect d. pinea in austrian pine (pinus nigra). hrma exploits d ...201121802051
pine needles as passive bio-samplers to determine polybrominated diphenyl ethers.eight polybrominated diphenyl ethers (pbdes) were determined in pine needles of three species (pinus halepensis, pinus pinea and pinus nigra) collected in the ne spain in an attempt to use this matrix for the biomonitoring of airborne pbdes. the method used was based in ultrasonic extraction followed by alumina and florisil clean-up and determination by gas chromatography coupled to mass spectrometry in negative chemical ionization. recoveries were between 99% and 138%, limits of detection betwe ...201121742367
Effects of pre-treatment on the nitrogen isotope composition of Japanese black pine (Pinus thunbergii) tree-rings as affected by high N input.Temporal changes in the acquisition of nitrogen (N) are recorded in tree-rings together with unique N isotopic values. Some debate continues regarding the importance of wood pre-treatment in isotope analysis and, thus, this study focuses on the removal of labile components to determine the intrinsic nature of N in tree-rings. The total concentration and stable isotopic value of N in annual tree-rings were determined for two cores from Japanese black pine (Pinus thunbergii) from areas colonized b ...201122006393
effect of environmental factors on the productivity of crimean pine (pinus nigra ssp. pallasiana) in sutculer, turkey.this study was conducted on pinus nigra sub sp. pallasiana commonly known as crimean pine. the purpose of this study was to determine the important site factors affecting the development of these forests in sutculer district. this district experiences a transitional climate between mediterranean and continental regions. there is a strong water deficit in summer. the data was collected from 37 sample plots. it was analyzed statistically by using upper stand height as a dependent variable and some ...200920329391
effect of pine mistletoe on radial growth of crimean pine (pinus nigra) in turkey.in this study, the influence of infection by pine mistletoe (viscum album l. subsp. austriacum (wiesb.) volmann) on the radial growth of crimean pine (pinus nigra amold) in turkey was investigated. we built local residual tree-ring-width chronologies using dendrochronogical techniques. tree ring chronologies of uninfected (control) crimean pine were used to estimate potential radial growth characteristics in the "infected" crimean pine (light, moderate and severe infection groups). in 2005, incr ...201122167935
identification of volatiles from pinus silvestris attractive for monochamus galloprovincialis using a spme-gc/ms platform.a myriad of volatile organic compounds (vocs) released by terrestrial vegetation plays an important role in environmental sciences. a thorough chemical identification of these species at the molecular level is essential in various fields, ranging from atmospheric chemistry to ecology of forest ecosystems. in particular, the recognition of vocs profiles in a context of plant-insect communication is a key issue for the development of forest protection tools.201122322293
the role of wood-inhabiting bacteria in pine wilt disease.the pathogenicity of the pine wood nematode (pwn), bursaphelenchus xylophilus together with the bacteria isolated from black pine (pinus thunbergii) bark inoculated to axenic black pine seedlings, significantly exceeded that of the axenic pwns alone, demonstrating that the bacteria play an important role in pine wilt disease. inoculation of seedlings with bacteria-free culture filtrates of the seven isolates from the dead seedlings from the above experiment showed that all isolate filtrates kill ...201123430766
ecological attributes and distribution of anatolian black pine [pinus nigra arnold. subsp. pallasiana lamb. holmboe] in turkey.the aim of this study is to determine ecoregions and the effect of ecological properties on natural distribution of anatolian black pine [pinus nigra arnold. subsp. pallasiana lamb. (holmboe)] in turkey. the results suggest that 6 ecoregions exist and climate, parent material, topography, anthropogenic factors, floristic composition, competition are ecological factors that determine the distribution of black pine in turkey. but, climatic elements such as precipitation and temperature are the dom ...201223424857
odor, gaseous and pm10 emissions from small scale combustion of wood types indigenous to central europe.in this study, we investigated the emissions, including odor, from log wood stoves, burning wood types indigenous to mid-european countries such as austria, czech republic, hungary, slovak republic, slovenia, switzerland, as well as baden-württemberg and bavaria (germany) and south tyrol (italy). the investigations were performed with a modern, certified, 8 kw, manually fired log wood stove, and the results were compared to emissions from a modern 9 kw pellet stove. the examined wood types were ...201223471123
increase of root induction in pinus nigra explants using agrobacteria.wounding of explanted pinus nigra primary explants followed by infection with agrobacterium rhizogenes wild strains 8196, 15834, or with the pria4abc transconjugant strain of a. tumefaciens (c58 chromosomal background) resulted in adventitious root induction. roots were formed in 60-97% of explants (1-3 roots/explant) but without a hairy root phenotype. the presence of t-dna of pri8196 or pria4abc in regenerated roots was confirmed by the opine (mannopinic acid) content. transformation response ...199624178528
biological activity of pinus nigra terpenes--evaluation of ftsz inhibition by selected compounds as contribution to their antimicrobial activity.in the current work, in vitro antioxidant, antibacterial, and antifungal activites of the needle terpenes of three taxa of pinus nigra from serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed. the black pine essential oils showed generally weak antioxidative properties tested by two methods (dpph and abts scavenging assays), where the highest activity was identified in p. nigra var. banatica (ic50=25.08 mg/ml and vitc=0.67 mg (vitamin c)/g when tested with the dpph and abts rea ...201425217763
pathogenicity of the pinewood nematode, bursaphelenchus xylophilus, to japanese larch, larix kaempferi, seedlings.pathogenicity of the pine wood nematode, bursaphelenchus xylophilus, to japanese larch, larix kaempferi, seedlings was tested with inoculation experiments under nursery conditions. water suspensions of nematodes (mixed stages cultured on botrytis cinerea or dispersal fourth-stage juveniles (dj4) extracted from the adult japanese pine sawyer, monochamus alternatus) were injected into the stems of 2- and 3-year-old japanese larch and japanese black pine, pinus thunbergii, seedlings growing in a nu ...200922661789
fungi isolated from pine wood nematode, its vector japanese pine sawyer, and the nematode-infected japanese black pine wood in korea.pine tree death caused by pine wood nematode (pwn) involves phoretic relationships between pwn and its vector japanese pine sawyer beetle (jps). in an effort to understand the diversity of fungi involved in pwn life cycle, a total of 176 fungal isolates were collected from pwns, adults and larvae of jps, pwn-diseased japanese black pine that was cut down in 2005 at jinju, korea. based on microscopic observation and colony morphology, and sequence analysis of the its rdna, the fungal isolates wer ...200724015089
a demographic analysis of mortality caused by the pine wood nematode (bursaphelenchus xylophilus) and pine sawyer beetles (monochamus alternatus) in pine forests in the seto inland sea-side, japan.demographic characteristics were examined in 51 study sites in naturally occurring pine populations in the seto inland sea-side. the pine forests consisted on average of 51.4% japanese red pine,pinus densiflora, 28.9% japanese black pine,pinus thunbergii, and 19.7% hybrids of the two species, and occurred along an elevatinal gradient from denser, younger secondary forest regrowing after disturbance, to more mature stands at higher elevations. the proportion of dead trees did not vary much among ...198628311773
reducing exposure to pathogens in the horse: a preliminary study into the survival of bacteria on a range of equine bedding types.to compare the rate of growth of four microbial strains that cause disease in the horse, on four commonly used types of bedding. the moisture-holding capacity of each bedding type was also tested.201727659898
austrian pine phenolics are likely contributors to systemic induced resistance against diplodia pinea.the molecular basis of the systemic induced resistance (sir) phenotype known to occur in austrian pine (pinus nigra j.f. arnold) in response to the tip blight and canker pathogen diplodia pinea (desm.) remains unclear. specialized metabolites such as phenolics are considered to be an important component of plant defense, including sir, but the antimicrobial activity of many of these putative defensive chemicals remains untested at realistic concentrations and in conjunction with each other. here ...201323999139
mechanisms of induced susceptibility to diplodia tip blight in drought-stressed austrian pine.plants experiencing drought stress are frequently more susceptible to pathogens, likely via alterations in physiology that create favorable conditions for pathogens. common plant responses to drought include the production of reactive oxygen species (ros) and the accumulation of free amino acids (aas), particularly proline. these same phenomena also frequently occur during pathogenic attack. therefore, drought-induced perturbations in aa and ros metabolism could potentially contribute to the obs ...201525900028
ophiostomatoid fungi including two new fungal species associated with pine root-feeding beetles in northern spain.many bark beetles live in a symbiosis with ophiostomatoid fungi but very little is known regarding these fungi in spain. in this study, we considered the fungi associated with nine bark beetle species and one weevil infesting two native tree species (pinus sylvestris and pinus nigra) and one non-native (pinus radiata) in cantabria (northern spain). this included examination of 239 bark beetles or their galleries. isolations yielded a total of 110 cultures that included 11 fungal species (five sp ...201425253585
greek pinus essential oils: larvicidal activity and repellency against aedes albopictus (diptera: culicidae).the needle volatiles metabolites of seven pinus spp.: pinus nigra (3 samples), pinus stankewiczii, pinus brutia, pinus halepensis, pinus canariensis, pinus pinaster and pinus strobus from greece were determined by gas chromatography and gas chromatography-mass spectrometry. p. nigra and p. canariensis essential oils were dominated by α-pinene (24.9-28.9 % and 15 %, respectively) and germacrene d (20.3-31.9 % and 55.8 %, respectively), whereas p. brutia and p. strobus by α-pinene (20.6 % and 31.4 ...201525399814
relationship of aluminium and calcium to net co2 exchange among diverse scots pine provenances under pollution stress in poland.light-saturated net photosynthesis (asat), dark respiration (rd), and foliar nutrient content of eight european scots pine (pinus sylvestris l.) provenances were measured at experimental sites in western poland. two-year-old seedlings were planted in 1984 at two sites with similar soils in areas of contrasting air pollution. one site was near a point source of so2 and other pollutants, and another 12 km to the southeast in an area free of acute air pollution was treated as a control. the eight p ...199428313592
physiological influence of silica on germinating pollen as shown by raman spectroscopy.the process of silicification in plants and the biochemical effects of silica in plant tissues are largely unknown. to study the molecular changes occurring in growing cells that are exposed to higher than normal concentration of silicic acid, raman spectra of germinating pollen grains of three species (pinus nigra, picea omorika, and camellia japonica) were analyzed in a multivariate classification approach that takes into account the variation of biochemical composition due to species, plant t ...201727174545
indicator species of essential forest tree species in the burdur district.the forests of burdur district for long have been subjected to over grazing and individual selection. as a result of this, majority of the forest areas in the district were degraded. in the district, afforestation efforts included majority of forestry implementations. it is well known that selecting suitable species plays an important role for achieving afforestation efforts. in this context, knowing the indicator species among the target species would be used in afforestation efforts, studies o ...201526591889
Phenotypic plasticity facilitates resistance to climate change in a highly variable environment.Increased summer drought will exacerbate the regeneration of many tree species at their lower latitudinal and altitudinal distribution limits. In vulnerable habitats, introduction of more drought-tolerant provenances or species is currently considered to accelerate tree species migration and facilitate forest persistence. Trade-offs between drought adaptation and growth plasticity might, however, limit the effectiveness of assisted migration, especially if introductions focus on provenances or s ...201122081261
appraisal on the wound healing and anti-inflammatory activities of the essential oils obtained from the cones and needles of pinus species by in vivo and in vitro experimental models.according to ethnobotanical data, pinus species have been used against rheumatic pain and for wound healing in turkish folk medicine. essential oils from the cones and needles of five different pinus species (pinus brutia ten., pinus halepensis mill., pinus nigra arn., pinus pinea l. and pinus sylvestris l.) were evaluated for their in vivo wound healing and anti-inflammatory activities.201122155393
trees as bioindicator of heavy metal pollution in three european cities.concentrations of four heavy metals were determined in tree leaves and bark collected from polluted and non-polluted areas of three european cities (salzburg, belgrade and thessaloniki) for a comparative study. platanus orientalis l. and pinus nigra arn., widespread in urban northern and southern europe, were tested for their suitability for air quality biomonitoring. leaves and barks were collected uniformly of an initial quantity of about 30 g of each sample. analysis was accomplished by elect ...201121907471
studies on the food and feeding habits of soil oribatei in a black pine plantation.food preferences and feeding behaviour of abundant species of oribatid mites in a black pine plantation have been studied by soil sectioning technique. it is suggested that microtritia minima, rhysotritia duplicata and phthiracarus sp. play an important role in the mechanical breakdown of decaying litter and, by contributing to the humification process, help in the accumulation of plant nutrients. due to poor mobility their contribution to fungal spore dissemination is insignificant. chamobates ...197328308239
improved aflp protocol using dual-suppression pcr and its application to species with large genomes.to improve the amplified fragment length polymorphism assay, dual-suppression pcr was introduced into the preamplification step of the assay. the dual-suppression pcr blocked completely the amplification of fragments with the same sequence (bsp1407i-bsp1407i or nlaiii-nlaiii) at both ends and amplified selectively fragments with different adaptor sequences (bsp1407i-nlaiii) at each end. two protocols, referred to as a and b, were established for species with medium- and large-sized genomes, resp ...201121676205
acute larvicidal toxicity of five essential oils (pinus nigra, hyssopus officinalis, satureja montana, aloysia citrodora and pelargonium graveolens) against the filariasis vector culex quinquefasciatus: synergistic and antagonistic effects.mosquito vector control is facing a number of important and timely challenges, mainly due to the rapid development of pesticide resistance and environmental concerns. in this scenario, screening of botanical resources for their mosquitocidal activity may offer effective and eco-friendly tools against culicidae vectors. culex quinquefasciatus say (diptera: culicidae) is a vector of lymphatic filariasis and of dangerous arboviral diseases, such as west nile and st. louis encephalitis. in this stud ...201728110082
decomposition of beech (fagus sylvatica) and pine (pinus nigra) litter along an alpine elevation gradient: decay and nutrient release.litter decomposition is an important process for cycling of nutrients in terrestrial ecosystems. the objective of this study was to evaluate direct and indirect effects of climate on litter decomposition along an altitudinal gradient in a temperate alpine region. foliar litter of european beech (fagus sylvatica) and black pine (pinus nigra) was incubated in litterbags during two years in the hochschwab massif of the northern limestone alps of austria. eight incubation sites were selected followi ...201526240437
description of cryptaphelenchus iranicus n. sp. (nematoda: ektaphelenchinae) recovered from bark samples of pinus nigra from iran.cryptaphelenchus iranicus n. sp., recovered from bark and wood samples of a weakened pinus nigra in kermanshah province, western iran, is described. the new species has females with body length of 250-330 µm and males 230-275 µm long, lip region set-off from body contour, 7-8 μm long stylet with small basal swellings, excretory pore located at 1.5-2.0 body diam. posterior to median bulb, post-vulval uterine sac short, 7-10 µm long and conical female posterior body end (tail) ending to a pointed ...201627470789
<i>lluciapomaresius</i> <i>nisae</i>, a new species of ephippigerini (orthoptera: tettigoniidae: bradyporinae) from the northeast of the iberian peninsula.a new species of the genus lluciapomaresius barat, 2012 is described from serra de llaberia in catalonia (in the northeast of iberian peninsula). lluciapomaresius nisae n. sp. was collected in a mediterranean pine forest dominated by european black pine (pinus nigra) and secondarily by calcicolous rosemary scrub. l. nisae is compared to l. panteli (navàs, 1899) from which it can be separated mainly by the shape of the male cerci, the titillators and the male calling song. also in the females by ...201728187677
description of phloeosinus laricionis sp. n. (coleoptera: curculionidae, scolytinae), a new bark beetle species from southern europe.we describe a new species of pine bark beetle, phloeosinus laricionis, associated with a pine decline recently affecting young plantations of pinus nigra ssp. laricio poiret growing on the etna volcano, sicily (south italy). the new species is morphologically close to the group species of p. cedri brisout, p. acatayi schedl, and p. pfefferi knížek, having all odd interstriae on the declivity of elytra bearing small, individual, sparse, more or less sharply pointed tubercles in males, or with sma ...201326171518
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