| expression, crystallization and preliminary x-ray analysis of an anomeric inverting agarase from pseudoalteromonas sp. cy24. | agab from pseudoalteromonas sp. cy24 is a novel agarase that hydrolyzes agarose to generate products with inverted anomeric configuration and that has been proposed to have a larger catalytic cleft than other β-agarases. here, the expression, purification, crystallization and data collection of agab in both wild-type and selenomethionine-substituted forms is described. the crystals of wild-type agab diffracted to 1.97 å resolution and belonged to space group c222(1). the selenomethionine derivat ... | 2010 | 21139213 |
| low-molecular-mass purine nucleoside phosphorylase: characterization and application in enzymatic synthesis of nucleoside antiviral drugs. | purine nucleoside phosphorylase (pnp) that catalyzes the reversible phosphorolysis of various purine nucleosides is widely distributed in prokaryotes and eukaryotes. four pnp genes from bacillus subtilis 168, escherichia coli k-12 and pseudoalteromonas sp. xm2107 were cloned by pcr and expressed in e. coli xl1-blue. recombinant pnps (rpnps) were purified by ni(2+)-nta chromatography. compared with other rpnps, pnp(816) was a low-molecular-mass homotrimer, which exhibited 11-, 4- and 1.5-fold hig ... | 2011 | 21279421 |
| purification and characterization of a bifunctional alginate lyase from pseudoalteromonas sp. sm0524. | an alginate lyase-producing bacterial strain, pseudoalteromonas sp. sm0524, was screened from marine rotten kelp. in an optimized condition, the production of alginate lyase from pseudoalteromonas sp. sm0524 reached 62.6 u/ml, suggesting that strain sm0524 is a good producer of alginate lyases. the bifunctional alginate lyase aly-sj02 secreted by strain sm0524 was purified. aly-sj02 had an apparent molecular mass of 32 kda. the optimal temperature and ph of aly-sj02 toward sodium alginate was 50 ... | 2011 | 21339950 |
| correlation between pigmentation and larval settlement deterrence by pseudoalteromonas sp. sf57. | the red-pigmented marine bacterium pseudoalteromonas sp. sf57 forms a biofilm that deters larval settlement of the tube-building polychaete hydroides elegans. to investigate the correlation between pigmentation and larval settlement deterrence, mutants of sf57 with deficient or altered pigmentation were generated by transposon mutagenesis. five groups of pigmented mutants were obtained, viz. white, yellow, pink, dark red, and white-to-red. the white mutant wm1, which exhibited a substantial incr ... | 2011 | 21390913 |
| characterization of a gene cluster and its putative promoter region for violacein biosynthesis in pseudoalteromonas sp. 520p1. | violacein, a purple pigment produced by some gram-negative bacteria, has various physiological properties, such as antitrypanosomal and antitumoral activities. a gene cluster that encodes five enzymes, vioa-vioe, is responsible for synthesizing violacein. the expression of these enzymes is known to be regulated by a quorum sensing mechanism in chromobacterium violaceum and pseudoalteromonas sp. 520p1. to clarify the molecular mechanism of regulation of violacein synthesis, we cloned and characte ... | 2011 | 21472536 |
| [laser tweezers raman spectroscopy analysis of cold-adapted aromatic hydrocarbons-degradating strains isolated from antarctic sea]. | laser tweezers raman spectroscopy can help with observing and studying individual cells or organelles in a natural state for a relatively long period. in the present experiment, laser tweezers raman spectroscopy (ltrs) was used as a tool to report physiological metabolism such as cells growth and nucleic acid, proteins, lipid and glucose of a single active cold-adapted aromatic hydrocarbons-degradating strains isolated from antarctic sea. after the raman spectrum was collected and analyzed, the ... | 2011 | 21510394 |
| characterization of a cryptic plasmid psm429 and its application for heterologous expression in psychrophilic pseudoalteromonas. | abstract: background: pseudoalteromonas is an important genus widespread in marine environment, and a lot of psychrophilic pseudoalteromonas strains thrive in deep sea and polar sea. by now, there are only a few genetic systems for pseudoalteromonas reported and no commercial pseudoalteromonas genetic system is available, which impedes the study of pseudoalteromonas, especially for psychrophilic strains. the aim of this study is to develop a heterologous expression system for psychrophilic pseud ... | 2011 | 21542941 |
| influence of a bacteriophage on the population dynamics of toxic dinoflagellates by lysis of algicidal bacteria. | a lytic phage (øzcw1) was isolated from an algicidal bacterium pseudoalteromonas sp. strain sp48 that specifically kills the toxic dinoflagellate alexandrium tamarense. we demonstrated that øzcw1 could trigger the growth of a. tamarense by inhibiting the growth of algicidal bacterium sp48. in contrast, the growth of a. tamarense was suppressed when cocultured with either sp48 or the øzcw1-resistant mutant of sp48. this study provides the first evidence of the indirect impact of bacteriophage on ... | 2011 | 21890676 |
| characterisation of the microbiota of atlantic mackerel (scomber scombrus). | in this study the microbiota of atlantic mackerel (scomber scombrus) collected by a commercial purse seiner was examined. fish were collected directly from the purse seine and from the refrigerated sea water (rsw) transport tank after loading. the culturable microbiota and specific spoilage bacteria (ssb) were quantified on iron agar lyngby (ial) and identified using commercially available biochemical api® kits on pure cultured isolates. these kits showed to be sub-optimal in characterising the ... | 2011 | 21914558 |
| the anti-biofilm activity secreted by a marine pseudoalteromonas strain. | bacterial biofilms occur on all submerged structures in marine environments. the authors previously reported that the marine bacterium pseudoalteromonas sp. 3j6 secretes antibiofilm activity. here, it was discovered that another pseudoalteromonas sp. strain, d41, inhibited the development of strain 3j6 in mixed biofilms. confocal laser scanning microscope observations revealed that the culture supernatant of strain d41 impaired biofilm formation of strain 3j6 and another marine bacterium. a micr ... | 2011 | 21895460 |
| isolation and characterization of a novel agarase-producing pseudoalteromonas spp. bacterium from the guts of spiny turban shells. | an agar-degrading bacterium was isolated from the guts of spiny turban shells. it was identified as a pseudoalteromonas species and named pseudoalteromonas sp. jybcl 1. the viscosity of the inoculated agar medium decreased by more than 60% after 20 h cultivation. the agarase produced by the isolate had optimal activities at 35 degrees c and ph 7. the enzyme had extremely strong resistance to ionic stress compared with other known agarases. its molecular mass was estimated at about 60 kda by sodi ... | 2011 | 21876371 |
| structure and activity of exo-1,3/1,4-β-glucanase from marine bacterium pseudoalteromonas sp. bb1 showing a novel c-terminal domain. | following the discovery of an exo-1,3/1,4-β-glucanase (glycoside hydrolase family 3) from a seaweed-associated bacterium pseudoalteromonas sp. bb1, the recombinant three-domain protein (exop) was crystallized and its structure solved to 2.3 å resolution. the first two domains of exop, both of which contribute to the architecture of the active site, are similar to those of the two-domain barley homologue β-d-glucan exohydrolase (exoi) with a distinctive trp-trp clamp at the +1 subsite, although e ... | 2011 | 22129429 |
| marine bacteria from danish coastal waters show antifouling activity against the marine fouling bacterium pseudoalteromonas sp. strain s91 and zoospores of the green alga ulva australis independent of bacteriocidal activity. | the aims of this study were to determine if marine bacteria from danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. we further assess if antibacterial effect is a good proxy for antifouling activity. we isolated 110 bacteria with anti-vibrio activity from different sample types and locations during a 1-year sampling from danish coastal waters. the strains were identified as pseudoalteromonas, phaeobacter, and vibrionac ... | 2011 | 22003011 |
| coastal seawater bacteria harbor a large reservoir of plasmid-mediated quinolone resistance determinants in jiaozhou bay, china. | diversity and prevalence of plasmid-mediated quinolone resistance determinants were investigated in environmental bacteria isolated from surface seawater of jiaozhou bay, china. five qnr gene alleles were identified in 34 isolates by pcr amplification, including qnra3 gene in a shewanella algae isolate, qnrb9 gene in a citrobacter freundii isolate, qnrd gene in 22 proteus vulgaris isolates, qnrs1 gene in 1 enterobacter sp. and 4 klebsiella spp. isolates, and qnrs2 gene in 1 pseudomonas sp. and 4 ... | 2012 | 22252223 |
| probiont niche specialization contributes to additive protection against vibrio owensii in spiny lobster larvae. | the development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen-probiont-host interactions. the probiont combination of pseudoalteromonas sp. pp107 and vibrio sp. pp05 provides additive protection against vectored vibrio owensii dy05 infection in larvae (phyllosomas) of ornate spiny lobster, panulirus ornatus. here, fluorescently tagged strains were used to demonstrate niche specialization of these probionts in both th ... | 2012 | 23757129 |
| [nonribosomal peptides synthetases gene clusters and core domain in pseudoalteromonas sp. nj631]. | we studied nonribosomal peptides synthetases (nrpss) gene clusters and the core module of nrpss in pseudoalteromonas sp. nj631 using genome mining approach. | 2012 | 23457803 |