| [effects of hormone treatment, light and temperatures on sprouting characteristics of bupleurum chinense]. | seeds of bupleurum chinense cultivar, zhongchai no. 1, were sowed in plastic pots which used the arable layer soil as the nursery bed and putted in the artificial climate incubator at various temperatures (15, 20, 25, 15-25 degrees c) and light (8,12 h) to germinate, respectively. the lower constant temperature (15 degrees ) and the higher constant temperature (25 "c) were not conducive to the sprouting characteristics of b. chinese. while they were able to enhance root activity to some extent; ... | 2014 | 25039172 |
| cell type-specific qualitative and quantitative analysis of saikosaponins in three bupleurum species using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry. | cell type-specific metabolite analysis is a promising method for understanding plant metabolite production, function, transport and storage. in the present study, laser microdissection (lmd) and ultra-high performance liquid chromatography quadrupole/time of flight-mass spectrometry are combined to determine where secondary metabolites are accumulated in the roots of bupleurum scorzonerifolium willd, bupleurum chinense dc. and bupleurum falcatum l. four tissues, namely cork, cortex, phloem and x ... | 2014 | 24863374 |
| inhibition of inflammatory injure by polysaccharides from bupleurum chinense through antagonizing p-selectin. | p-selectin-mediated adhesion between endothelium and neutrophils is a crucial process leading to acute inflammatory injure. thus, p-selectin has been considered as promising target for therapeutics of acute inflammatory-related diseases. in the present study, the water-soluble polysaccharides (bcps) were isolated from bupleurum chinense, and we evaluated their therapeutical effects on acute inflammatory injure and antagonistic function against p-selectin-mediated neutrophil adhesion. our results ... | 2014 | 24708947 |
| [studies on high efficient plant regeneration system from anther callus of bupleurum chinense]. | the callus of bupleurum chinense with anthers at the stage of uninucleate was induced. after several subcultures, anther calli of b. chinense were cultured at 20 ms culture mediums with different plant hormones to differentiate into plantlets. differentiation of callus was detected after 21 and 49 days to select the most effective medium. there were 19 culture mediums in which anther callus could differentiate into plantlets with differentiation rate range from 3% to 60% , and most less than 20% ... | 2013 | 24494550 |
| [induction of hairy roots and plantlet regeneration of bupleurum chinense dc]. | in this study, the induction of hairy roots of bupleurum chinense dc. was explored and established after experiments at different conditions: a. rhizogenes a4 was used to infect the leaves bases of b. chinense tube seedlings. the explants were co-cultured on phytagel-solidified media for 3 days and then, were turned into solid media, similar with the co-culture media except that bacteriostat was added. after 10 days, rootlets began to appear and after 4 to 5 weeks, rootlets can be converted into ... | 2013 | 24358786 |
| herbal medicines for fatty liver diseases. | fatty liver disease is potentially a reversible condition that may lead to end-stage liver disease. since herbal medicines such as crataegus pinnatifida and salvia miltiorrhiza have increasingly been used in the management of fatty liver disease, a systematic review on herbal medicine for fatty liver disease is needed. | 2013 | 23975682 |
| herb-induced autoimmune-like hepatitis in c57bl/6j mice. | animal model suitable for studying herb-induced experimental autoimmune hepatitis (aih) remains a challenging problem. a medicinal herb containing scutellaria baicalensis georgi (sb) and bupleurum chinense dc (bc) has been sporadically reported to be related to liver fibrosis. the aim of this study was to investigate the effects of sb and bc on experimental aih in mice. | 2014 | 23890230 |
| isolation of an anti-complementary polysaccharide from the root of bupleurum chinense and identification of its targets in complement activation cascade. | to isolate and characterize the anti-complementary polysaccharide from the root of bupleurum chinense. | 2013 | 23787186 |
| an anti-complementary polysaccharide from the roots of bupleurum chinense. | bioactivity-guided fractionation of hot-water extract from the roots of bupleurum chinense dc led to the isolation of a homogeneous polysaccharide bc-ps1 composed of gal, gala, glc, ara and man in the ratio of 1.6:1.1:1.8:1.7:1.0, along with trace of rha, fuc and xyl. the main linkages of the residues of bc-ps1 include terminal, 1,4-linked and 1,3-linked galp (gala), terminal and 1,4-linked glcp, terminal and 1,5-linked araf, 1,4,6-linked manp. bioassays revealed that the concentrations of bc-ps ... | 2013 | 23570674 |
| saikosaponin a enhances transient inactivating potassium current in rat hippocampal ca1 neurons. | saikosaponin a (ssa), a main constituent of the chinese herb bupleurum chinense dc., has been demonstrated to have antiepileptic activity. recent studies have shown that ssa could inhibit nmda receptor current and persistent sodium current. however, the effects of ssa on potassium (k(+)) currents remain unclear. in this study, we tested the effect of ssa on 4ap-induced epileptiform discharges and k(+) currents in ca1 neurons of rat hippocampal slices. we found that ssa significantly inhibited ep ... | 2013 | 23554830 |
| osteoclast-inhibiting saikosaponin derivatives from bupleurum chinense. | five new saikosaponins, saikosaponin w (1), 21β-hydroxysaikosaponin b2 (2), 6″-o-acetylsaikosaponin e (3), 6″-o-acetylsaikosaponin b1 (4), and 6″-o-acetylsaikosaponin b3 (5), along with twenty-two known ones (6-27), have been isolated from the roots of bupleurum chinense. their structures were elucidated on the basis of detailed spectroscopic analysis, including mainly 1d and 2d nmr and hresi-ms, qualitative chemical methods, and comparison with the literatures. osteoclast-inhibiting activity of ... | 2013 | 23333582 |
| antioxidant activity and hepatoprotective effect of a polysaccharide from bei chaihu (bupleurum chinense dc). | chinese medicine plays a pivotal role in hepatoprotective treatment. in the present study, a water-soluble polysaccharide fraction (wbcp) was fractioned from the roots of bupleurum chinense and purified by deae-cellulose and surperdex 200 hr chromatography. the physicochemical properties, antioxidative and hepatoprotective activities of wbcp were evaluated in a rat model of hepatic injury caused by d-galactosamine (galn). hepatoprotective effect was evaluated by measuring aspartate transaminase ... | 2012 | 24750742 |
| [cloning of ugt gene of bupleurum chinense and construction of over expressing and rnai transgenic vectors]. | to clone the full-length cdna of a uridine diphosphate glycosyltransferase (ugt) gene which may be involved in saikosaponin biosynthesis of bupleurum chinense, and construct the transgenic vectors for over expression and rnai of the cloned ugt. these works will provide foundation for further its function analysis by transgene study. | 2012 | 22693893 |
| discovery of gaba(a) receptor modulator aristolactone in a commercial sample of the chinese herbal drug "chaihu" (bupleurum chinense roots) unravels adulteration by nephrotoxic aristolochia manshuriensis roots. | in a two-microelectrode voltage clamp assay using xenopus laevis oocytes, a petroleum ether extract prepared from a commercial sample of the traditional chinese herbal drug labelled as " chaihu" (bupleurum chinense dc. roots) enhanced the i(gaba) by 156 % ± 22 % when tested at 100 µg/ml. by means of hplc-based activity profiling combined with high-resolution lc-ms and microprobe nmr, the germacranolide aristolactone was identified as one of the main active compounds (ec₅₀ 56.02 µm ± 5.09 µm). ho ... | 2012 | 22271081 |