| synthesis of oligosaccharide derivatives related to those from sanqi, a chinese herbal medicine from panax notoginseng. | oligosaccharide derivatives from sanqi, a chinese herbal medicine derived from panax notoginseng, methyl beta-d-galactopyranosyl-(1-->3)-[alpha-l-arabinofuranosyl-(1-->6)]-alpha-d-galactopyranoside, diosgenyl beta-d-galactopyranosyl-(1-->3)-[alpha-l-arabinofuranosyl-(1-->6)]-alpha-d-galactopyranoside, and methyl beta-d-galactopyranosyl-(1-->3)-[alpha-l-arabinofuranosyl-(1-->6)]-alpha-d-galactopyranosyl-(1-->4)-beta-d-galactopyranosyl-(1-->3)-[alpha-l-arabinofuranosyl-(1-->6)]-alpha-d-galactopyra ... | 2002 | 11890886 |
| [application study of yangkang biological fertilizer on panax notoginseng cultivation]. | yangkang biological fertilizer was sprayed on panax notoginseng. the result indicated that the yield and the surviving rate can be raised 25.2% and 15.2% respectively. the best spraying practice were: in the unfolding stage of leaves (april) and bloom stage (july), with yangkang biological fertilizer plus water (1:400), at four times every stage. | 1999 | 12571908 |
| estrogen-like activity of ginsenoside rg1 derived from panax notoginseng. | ginsenosides have demonstrated pharmacological effects in the central nervous, cardiovascular, and endocrine systems. we hypothesize that ginsenosides might mediate some of their actions by binding to the estrogen receptor, as they share many of the protective actions of estrogen in various physiological systems. the present study is aimed to determine whether ginsenoside rg1 can act like an estrogen analog in stimulating human breast cancer cell growth as well as in the activation of estrogen r ... | 2002 | 12161497 |
| [cerebral protective effects of some compounds isolated from traditional chinese herbs]. | to study the cerebral protective effects of some compounds isolated from traditional chinese herbs. | 1999 | 12205950 |
| [effects of rg1 on calcium channel of guinea pig ventricular myocytes]. | to evaluate the effect of rg1 (purified saponin of panax notoginseng) on l-ca2+ channel of guinea pig ventricular myocytes. | 1999 | 12205963 |
| [effect of panax notoginseng saponins combined isovolumic haemodilution on the retinal microcirculation of patients with retinal vein occlusion]. | to study the effect of panax notoginseng saponins (pns) or isovolumic haemodilution (ihd) and combination of pns with ihd on retinal microcirculation of patients with retinal vein occlusion (rvo). | 2000 | 12206008 |
| protective effects of trilinolein extracted from panax notoginseng against cardiovascular disease. | trilinolein is a triacylglycerol purified from a commonly used traditional chinese medicine panax notoginseng. trilinolein has been reported to provide a number of beneficial effects including reducing thrombogenicity and arrhythmias and increasing erythrocyte deformability. additionally, trilinolein has been reported to be an antioxidant, which can counteract free radical damage associated with atherogenesis, and myocardial damage seen with ischaemia and reperfusion. these pharmacologic effects ... | 2002 | 12466054 |
| [determination of organochlorine pesticide multi-residues in astragalus membranaceus (fisch.) bge. var. mongholicus (bge.) hsiao, panax notoginseng (burk.) f. h. chen and p. quin quefolium l]. | to determine 15 organochlorine pesticide residues in astragalus membranaceus, panax notoginseng and p. quinquefolium. | 2000 | 12515220 |
| liquid chromatography/mass spectrometry of malonyl-ginsenosides in the authentication of ginseng. | different negative ion electrospray (es) source conditions are required to concentrate the ion current in [m-h](-) for malonylated and non-malonylated ginsenosides. however, both can be ionised optimally in a single liquid chromatography/mass spectrometry (lc/ms) analysis by employing switchable voltages in the post-source ion optics of a quadrupole ion trap mass spectrometer. coupled with automatic ms/ms scanning and post-acquisition neutral loss data analysis, this method provides a means of p ... | 2003 | 12539190 |
| [effects of panax notoginseng saponins on the expression of tumor necrosis factor alpha and secretion phospholipase a2 in rats with liver fibrosis]. | | 2003 | 12546751 |
| [studies on control of root rot on panax notoginseng]. | chemical control tests of pot, plot and field for panax notoginseng root rot were conducted during 1995-1996. the results indicated that the chemical control is a effect measure to control rapidly occurring and spreading of panax notoginseng root rot. it was the best treatment to coordinate use of bactericide and fungicide, obviously better than alone or mixed use of fungicide and also better than alone use of bactericide. in the pot and plot tests, the best coordinate treatment was the treatmen ... | 1998 | 12567944 |
| [analysis of the vestiges of farm chemicals for panax notoginseng]. | the vestiges of farm chemicals in panax notoginseng plants and medicinal materials from main production areas in wenshan district was analyzed in this study. the result showed that the samples from the places contained hexachloro-cyclohexane and ddt. and the vestiges of hexachloro-cyclohexane was about 0.016-0.101 mg/kg, that of ddt was about 0.15-0.143 mg/kg. the content of cu, zn, pb, cd was lower than national standard of p. r. c. | 1999 | 12575102 |
| [effects of cultivating measures on root rot of panax notoginseng]. | to probe into the effects of cultivating measures on root rot of panax notoginseng for the integrate control. | 2000 | 12575264 |
| [seedling quality and root rot on panax notoginseng]. | the experiment was carried on the relationship between seedling quality and root rot on sanqi (panax notoginseng). the result showed that seedlings from new reclamation land, the root rot disease was fewer and existing rate of seedling was higher than that of seedlings from old gardenplot. therefore, selecting ill-free and robust seedling is one of important measure for the control of root rot on sanqi cultivation. | 2002 | 12583185 |
| panax notoginseng (burk.) effects on fibrinogen and lipid plasma level in rats fed on a high-fat diet. | several studies have shown that notoginsenoides improve diastolic function in hypertensive subjects, induce the fibrinolytic system in in vitro models and act as antiproliferative agents on vessel leiomyocytes. our aim was to evaluate their effect on fibrinogen and lipid plasma levels compared with a well-known hmgcoa reductase inhibitor. seventy wistar male adult rats on a fat-enriched diet were treated orally with p. notoginseng pulverized root (43 mg/kg/day or 86 mg/kg/day; 20 animals per gro ... | 2003 | 12601683 |
| pharmacokinetics and bioavailability of ginsenoside rb1 and rg1 from panax notoginseng in rats. | panax notoginseng is used as a therapeutic agent to stop haemorrhages and a tonic to promote health in chinese medicine. currently saponins of p. notoginseng (pns) are especially given attentions for their hemorheological properties. the pharmacokinetic profiles of the main pns are still not accurately investigated. therefore, our preliminary aim is to elucidate the pharmacokinetic features of ginsenoside rb(1) (rb(1)) and ginsenoside rg(1) (rg(1)), two of the main pns in rats. firstly, quantiti ... | 2003 | 12648814 |
| analysis of saponins in raw and steamed panax notoginseng using high-performance liquid chromatography with diode array detection. | a reversed-phase high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of six saponins (notoginsenoside r1, ginsenosides rg1, re, rb1, rc, rd) in raw and steamed panax notoginseng. linearity (r2 > 0.9988), intra- and inter-day precision (rsd < 4%), limit of detection (0.008-0.013 mg/ml), limit of quantification (0.027-0.042 mg/ml) of the saponins were determined. the method was successfully applied to 11 pairs of raw an ... | 2003 | 14518765 |
| [observation of blooming habits of panax notoginseng]. | this paper reported the whole process of blooming habits of panax notoginseng and pollens scattering regularities in wenshan, yunnan province. it can provide reference for the pollenation of hybridization. | 2003 | 14528688 |
| [study on therapeutic window of opportunity for panax notoginseng saponins following focal cerebral ischemia/reperfusion injury in rats]. | to study the therapeutic window of opportunity for panax notoginseng saponins (pns) following focal cerebral ischemia/reperfusion injury in rats. | 2004 | 15179785 |
| haemolytic activities and immunologic adjuvant effect of panax notoginseng saponins. | to evaluate haemolytic activities and adjuvant effect of panax notoginseng saponins (pns). | 2003 | 14627501 |
| [the research of technique of panax notoginseng seed coating for culturing seedlings]. | to screen seed coating formulation and to investigate culturing seedling technique for panax notoginseng (sanqi). | 2002 | 12685341 |
| [protective effects and its mechanism of panaxatriol saponins isolated from panax notoginseng on cerebral ischemia]. | to study the protective effects and its mechanism of panaxatriol saponins isolated from panax notoginseng (pts) on focal cerebral ischemia in rat brain. | 2002 | 12774330 |
| [effect of dan-shen, san-qi of different proportion on platelet aggregation and adhesion in normal rabbits]. | to observe the effect of the crude extracts of dan-shen root and san-qi of different proportion on platelet aggregation and adhesion in normal rabbits. | 2002 | 12776499 |
| authentication of panax notoginseng by 5s-rrna spacer domain and random amplified polymorphic dna (rapd) analysis. | the great majority of panax species are well-known herbal medicines in the orient, and many of them share a close resemblance in appearance and chemical composition. among these panax species, the root of p. notoginseng (sanqi) is a unique herb that has distinct clinical usage. here, the 5s-rrna spacer domains were isolated from p. notoginseng, p. japonicus var. major, p. stipuleanatus, p. quinquefolius, p. ginseng, p. zingiberensis, and p. wangianus, and four common adulterants of p. notoginsen ... | 2003 | 12865989 |
| structures of new dammarane-type triterpene saponins from the flower buds of panax notoginseng and hepatoprotective effects of principal ginseng saponins. | the saponin fraction from the flower buds of panax notoginseng exhibited protective effect on liver injury induced by d-galactosamine and lipopolysaccharide. from the saponin fraction with hepatoprotective effect, five new dammarane-type triterpene saponins, notoginsenosides-o (1), -p (2), -q (3), -s (4), and -t (5), were isolated together with nine known protopanaxadiol oligoglycosides. the structures of the new saponins were elucidated on the basis of chemical and physicochemical evidence. the ... | 2003 | 12880307 |
| effects of panax notoginseng saponins on myocardial gsalpha mrna expression and atpase activity after severe scald in rats. | the aim of this study was to investigate the changes of myocardial gsalpha mrna expression and atpase and the effects of panax notoginseng saponins (pns) on that after burns in rats. wistar rats were exposed to a 95 degrees c water bath for 10s to produce 30% tbsa skin-full-thickness scalds. myocardial gsalpha mrna level, camp content and adenyl cyclase (ac) activities were determined with dot blotting hybridization, radioimmunoassay and indirect method, respectively. the atpase activities in pl ... | 2003 | 12927977 |
| isolation of dammarane saponins from panax notoginseng by high-speed counter-current chromatography. | the chinese phytomedicinal formulation sanqi zongdai pian, traditionally prepared from crude extracts from roots of panax notoginseng (araliaceae), contains highly polar dammarane saponins which were separated at a preparative scale using high-speed counter-current chromatography (hsccc). in each operation, 283 mg methanolic extract of five tablets was separated and yielded pure 157, 17, 13 and 56 mg of ginsenoside-rb1, notoginsenoside-r1, ginsenoside-re and ginsenoside-rg1, respectively, n-hexa ... | 2003 | 12967182 |
| studies on the interaction of total saponins of panax notoginseng and human serum albumin by fourier transform infrared spectroscopy. | total saponins of panax notoginseng (tpns), isolated from the roots of panax notoginseng (burk) f.h. chen, have been considered as the main active components of san-chi and have various therapeutical actions. their interactions with human serum albumin have been investigated by fourier transformed infrared spectrometry and fluorescence methods. the results showed that tpns combined with hsa through c=o and c-n groups of polypeptide chain. the drug-protein combination caused the significant loss ... | 2003 | 14499835 |
| effects of san qi on gastric secretion and protective factors of gastric mucosa in the rat with precancerous lesion of stomach. | in the model rat with precancerous lesion of stomach induced by the combined method of insertion of a spring into the pylorus and high salt hot paste, effects of san qi ([symbol: see text] radix notoginseng) on gastric secretion and protective factors of stomach were investigated. the results indicated that gastric secretion, gastric mucosal blood flow (gmbf) and aminohexose content lowered significantly, and malondialdehyde (mda) increased significantly (p < 0.01) in the model group as compared ... | 2003 | 14535198 |
| protection by hot water extract of panax notoginseng on chronic ethanol-induced hepatotoxicity. | the purpose of this study was to investigate the antioxidant effects of a hot water extract of panax notoginseng (png) against chronic ethanol-induced hepatotoxicity. fifty mice were divided into fi ve equal groups with 10 in each group. group 1 (control) received saline, whereas group 2 received ethanol (70%, 0.1 ml, p.o.) once daily for 4 weeks, which induced hepatotoxicity, manifested biochemically by a significant elevation of serum enzyme activities, such as sgot and sgpt. hepatotoxicity wa ... | 2003 | 14595601 |
| chemical assessment of roots of panax notoginseng in china: regional and seasonal variations in its active constituents. | root of panax notoginseng (radix notoginseng, sanqi) is a well-known traditional chinese medicine and is mainly cultivated in wenshan of yunnan, china. the active constituents include saponin, dencichine, flavonoid, and polysaccharide; however, the levels of these components vary in different geographical regions of growth and also show a seasonal variation. by using high-performance liquid chromatography and spectrophotometry, the contents of notoginsenoside r1, ginsenoside r(g1), r(b1), r(d), ... | 2003 | 14705886 |
| inhibitory effect of trilinolein on angiotensin ii-induced cardiomyocyte hypertrophy. | the myocardial protective effects of trilinolein, isolated from the chinese herb sanchi (panax notoginseng), may be related to its antioxidant effects. in the present study, we investigated the effects of trilinolein on angiotensin ii-induced cardiomyocyte hypertrophy. cultured neonatal rat cardiomyocytes were stimulated with angiotensin ii, [3h]leucine incorporation and the beta-myosin heavy chain promoter activity were examined. we also examined the effects of trilinolein on angiotensin ii-ind ... | 2004 | 14729376 |
| the inhibitory effect of trilinolein on norepinephrine-induced beta-myosin heavy chain promoter activity, reactive oxygen species generation, and extracellular signal-regulated kinase phosphorylation in neonatal rat cardiomyocytes. | the myocardial protective effects of trilinolein, isolated from the traditional chinese herb sanchi (panax notoginseng), are thought to be related to its antioxidant activity. however, the intracellular mechanism underlying the protective effect of trilinolein in the heart remains unclear. in the present study, we investigated the effect of trilinolein on norepinephrine (ne)-induced protein synthesis in cardiomyocytes. cultured neonatal rat cardiomyocytes were stimulated with ne, then protein co ... | 2004 | 14730205 |
| [a review of adverse effects of panax notoginseng (burk.) f.h.chen]. | | 2002 | 14748374 |
| [effect of panax notoginseng saponin on procoagulant activity and differentiation induction in nb4 cells]. | to investigate the effect of panax notoginseng saponin (pns) on procoagulant activity (pca) and differentiation induction in nb4 cells. | 2004 | 14976894 |
| [studies on formulations of panax notoginsenosides for intranasal administration]. | to develop high bioavailability preparations without irritation for panax notoginsenosides. | 2003 | 14992002 |
| [study on the anti-apopotosis induced by hypoxia/hypoglycemia and reoxygenation of panax notoginseng saponins in cultured rat hippocampal neurons]. | to study the inhibitory effects of panax notoginseng saponins(pns) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons. | 2003 | 15015268 |
| interactions of a herbal combination that inhibits growth of prostate cancer cells. | pc spes is an eight-component herbal product marketed for the treatment of prostate cancer. the manufacturer of pc spes claims that the herbal combination is a synergistic blend, but the purported synergy has never been tested. we examined the interaction in cell culture of these eight individual herbal components by the use of an isobologram. | 2004 | 15060741 |
| [effects of astragalus and saponins of panax notoginseng on mmp-9 in patients with type 2 diabetic macroangiopathy]. | to investigate the role and mechanism of astragalus (as) and saponins of panax notoginseng (pns) in treating type 2 diabetic macroangiopathy. | 2004 | 15706857 |
| [clinical observation on effect of xuesaitong injection as auxiliary treatment of severe craniocerebral injury]. | to evaluate the effect of xuesaitong injection (xsti, a preparation of panax notoginseng) as auxilliary treatment of severe craniocerebral injury. | 2004 | 15074087 |
| [identification of panax notoginseng and its preparations by lc/ms]. | to develop a method of identifying the existing of panax notoginseng in products of traditional chinese medicine compound danshen. | 2004 | 15127621 |
| [fingerprint construction of panax notoginseng]. | to provide application and identification basis for panax notoginseng by constructing the hplc-fp of panax notoginsing. | 2003 | 15139137 |
| [effect of radix notoginseng saponins on platelet activating molecule expression and aggregation in patients with blood hyperviscosity syndrome]. | in order to explore the relationship between the active components and the functional links of chinese herbs, the effect of xuesaitong capsule, a preparation made of multi-component panax notoginseng saponins (pns) on platelet activating molecule expression and aggregation in patients with blood hyperviscosity syndrome (bhs) was observed, with aspirin (asp) as a control. | 2004 | 15143716 |
| the antioxidant effects of aqueous and organic extracts of panax quinquefolium, panax notoginseng, codonopsis pilosula, pseudostellaria heterophylla and glehnia littoralis. | the roots of panax quinquefolium, panax notoginseng, glehnia littoralis, codonopsis pilosula and pseudostellaria heterophylla were extracted with an aqueous extraction method and also with an organic extraction method. the aqueous extracts of glehnia littoralis and codonopsis pilosula were the most potent in inhibiting erythrocyte hemolysis. the aqueous extracts of panax quinquefolium and panax notoginseng had lower potencies while the aqueous extract of pseudostellaria heterophylla and the orga ... | 2004 | 15234766 |
| [the historical condition in the spread of sanqi (panax notoginseng) in the ming dynasty]. | a well-known and extensively applied chinese herbs for promoting blood circulation and resolving blood stasis, san qi (pana notoginseng) is one of the medicinal plants first applied by the minority ethnic groups in southwestern china originally grown in wenshan of yunnan and baise of guangxi, china. the doctors in inland china began to know and apply it in the ming dynasty, indicating that the spread of medicine is restricted and influenced not only by geographical positions but also by social e ... | 2004 | 15555246 |
| liquid chromatographic method for determination of four active saponins from panax notoginseng in rat urine using solid-phase extraction. | four major active saponins (ginsenosides rg1, rb1, rd and notoginsenoside r1) in panax notoginseng were determined in rat urine after oral and intravenous administration of total saponins of p. notoginseng (pns), and the urine samples were treated with solid-phase extraction (spe) prior to liquid chromatography. a reversed-phase liquid chromatography system with ultraviolet detection and a zorbax sb-c18 column was used. the within-day and between-day assay coefficients of variation for the four ... | 2004 | 15261811 |
| [fingerprinting of panax notoginseng by high-performance liquid chromatography]. | fingerprinting of panax notoginseng was performed by high-performance liquid chromatography using agilent hypersil c18 (250.0 mm x 4.0 mm, 5 microm) column with the mobile phase of acetonitrile and water and gradient elution. the detection wavelength was set at 203 nm. the method is simple and reliable to identify and evaluate the quality of panax notoginseng. | 2004 | 15604072 |
| comparative study of the composition of tcm radix notoginseng extract samples before and after acetylation with uv and elsd detection. | to study the derivation, acetylation specifically, as well as the application of evaporative light-scattering detector (elsd) detection of panax notoginseng extract for a better understanding of its components, as well as to study the authentication of this traditional chinese medicine (tcm). | 2004 | 15605098 |
| [industrialization of medicinal plant tissue culture]. | compared with other countries, the industrialization of medicinal plant tissue culture is more important for our country because china is consuming and exporting the most amounts of herb materials in the world. each year, many papers and patents are published on cell cultures of popular medicinal plants, such as taxus sp., catharanthus roseus, and panax ginseng, and, meanwhile, the research on organ cultures of medicinal plants is increasing very quickly, which is deepening the study of medicina ... | 2003 | 15139118 |
| scale-up of centrifugal impeller bioreactor for hyperproduction of ginseng saponin and polysaccharide by high-density cultivation of panax notoginseng cells. | scale-up of a novel centrifugal impeller bioreactor (cib) was demonstrated for production of valuable plant-specific secondary metabolites by high-density cell cultures. initial kla was identified to be a key factor affecting cell growth and production of ginseng saponin and polysaccharide by high-density cultivation of panax notoginseng cells in a 3-l cib. a high level of ginseng saponin and polysaccharide production was obtained at an initial kla value of 30.2 h(-1). a maximum dry cell weight ... | 2004 | 15296432 |
| total saponins of panax notoginseng protected rabbit iliac artery against balloon endothelial denudation injury. | to investigate whether total panax notoginseng saponins (pns) could protect endothelium of rabbit iliac artery against balloon endothelial denudation (bed) injury. | 2004 | 15339390 |
| adjuvant effect of panax notoginseng saponins on the immune responses to ovalbumin in mice. | in this study, the haemolytic activities of panax notoginseng saponins (pns) and its adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (ova) were evaluated. we determined the haemolytic activity of pns using 0.5% rabbit red blood cell. pns showed a slight haemolytic effect, with its haemolytic percents being 11.59 and 3.60% at the concentrations of 500 and 250 microg/ml, respectively. furthermore, the adjuvant potential of pns at three dose levels on ... | 2004 | 15364435 |
| [effect of total saponins of panax notoginseng on transdifferentiation of rats' tubular epithelial cell induced by il-1alpha]. | to study whether total saponins of panax notoginseng (pns) can prevent renal interstitial fibrosis occurrence through blocking the il-1alpha induced tubular epithelial cell and decrease the secretion of extracellular matrix (ecm). | 2004 | 15366598 |
| hplc determination of four active saponins from panax notoginseng in rat serum and its application to pharmacokinetic studies. | four main active saponins (ginsenosides rg1, rb1, rd and notoginsenoside r1) in panax notoginseng in rat serum after oral and intravenous administration of total saponins of p. notoginseng (pns) to rats were determined using a simple and sensitive high-performance chromatographic method. the serum samples were pretreated with solid-phase extraction before analysis. the calibration curves for the four saponins were linear in the given concentration ranges. the intra-day and inter-day assay coeffi ... | 2004 | 15484230 |
| [effect of total panax notoginseng saponins on the activity of human endothelial nitric oxide synthase gene promoter]. | to study the mechanism of total panax notoginseng saponin (tpns) in regulating the transcription activity of human endothelial nitric oxide synthase (henos) gene promoter. | 2004 | 15485778 |
| [inhibiting effects of panax notoginseng extracts on proliferation of ges-1 cells and mnng-transformed ges-1 cells]. | through cell cultivation, we studied the inhibiting effects of the serum of the dog fed with panax notoginseng extracts on precancerous gastric cells, trying to find the best time points or periods when the extracts' function was the strongest after administration of the extracts to the dog. | 2004 | 15539025 |
| [isolation and identification of minor bioactive saponins from the leaves of panax notoginseng]. | the minor bioactive saponins of the total saponin from the leaves of panax notoginseng (burk.) f. h. chen. were separated and purified by column chromatographies on silica gel and thin layer gel. four compounds were identified as: ginsenoside c-k (i) [20 (s) -protopanaxadiol 20-o-beta-d-glucopyranoside], ginsenoside-rh1 (ii) [20 (s) -protopanaxatriol-6-o-beta-d-glucopyranoside], ginsenoside-mc (iii) [20 (s)-protopanaxadiol 20-o-alpha-l-arabinofuranosyl (1 --> 6) -d-glucopyranoside] and notoginse ... | 2004 | 15551962 |
| panax notoginseng supplementation enhances physical performance during endurance exercise. | the purpose of this study was to investigate whether a single 1,350-mg dose of panax notoginseng (png) could enhance aerobic capacity, endurance, and mean blood pressure (map) in young adults. we randomly assigned 29 untrained adults, aged 20-35 years, to an experimental (exp, n = 13) or a control (con, n = 16) group. for 30 days, the exp took 1,350 mg per day of png capsule and the con consumed 1,350 mg per day of starch capsule. measurement variables were taken before and after 30 days of eith ... | 2005 | 15744902 |
| [effects of panax notoginseng saponins on rat cardiomyocytes apoptosis induced by angiotengin ii in vitro]. | to study the protective effects of panax notoginseng saponins (pns) on angiotensin ii (ang ii)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. | 2005 | 16075720 |
| [apoptosis-promoting effect of panax notoginseng extracts on mnng-transformed ges-1 cells]. | to study the apoptosis-promoting effect of the serum from panax notoginseng extracts-fed dog on precancerous gastric cells by means of flow cytometry. | 2005 | 15763060 |
| identification of panax species in the herbal medicine preparations using gradient pcr method. | in order to identify the existence of panax species in herbal medicine preparations, the ginseng specific marker primer was selected and created based on the sequence of korean ginseng dna fragment, 359 bp. the gradient pcr was performed on 40 types of the herbal medicines including the 7 types of araliaceae that are in the same family with the panax ginseng using the created ginseng maker primer. as result, panax notoginseng (chinese), panax japonicus (japanese) and panax quinquefolius (america ... | 2005 | 15802808 |
| high-performance liquid chromatographic method with quantitative comparisons of whole chromatograms of raw and steamed panax notoginseng. | a high-performance liquid chromatographic (hplc) method coupled with chromatographic pattern matching was developed to differentiate whole chromatograms of raw and steamed panax notoginseng objectively and quantitatively. the major peaks differentiating chromatograms of raw and steamed samples were also identified for the first time in this herb. the raw and steamed p. notoginseng roots and its products were successfully differentiated. the quantitative differences between the chromatograms were ... | 2004 | 15584233 |
| [analysis on the geologic background and physicochemical properties of soil for the cultivation of panax notoginseng in yunnan province]. | to study the relationship between the geo-authentic and geologic background system (gbs) and physicochemical properties of soil for the cultivation of panax notoginseng. | 2005 | 15806962 |
| hydrophilic interaction liquid chromatography with tandem mass spectrometry for the determination of underivatized dencichine (beta-n-oxalyl-l-alpha,beta-diaminopropionic acid) in panax medicinal plant species. | dencichine (beta-n-oxalyl-l-alpha,beta-diaminopropionic acid) is a haemostatic agent present in important chinese medicinal herbs such as panax notoginseng, as well as other panax species. it is also a reported neurotoxic agent found in lathyrus sativus (grass pea seed). a selective analytical method incorporating hydrophilic interaction chromatography with positive electrospray ionization tandem mass spectrometry (hilic/esi-ms/ms), for the analysis of dencichine in panax plant species, was deve ... | 2005 | 15838924 |
| [effects of lingfasu on embryoid and plantlet formation of panax notoginseng in vitro]. | on the medium ms added the right amount of 2,4-d and lfs (angustmycin) and cultured under dark condition, the callus from stem segments of panax notoginseng could induce a lot of embryoids. in 2-3 months, the ratio of embryoid formation reached about 90%. then transplanted on ms + 2,4-d 1.5 mg/l + lfs 2 mg/l and cultured under light 20001x, near 30% embryoids could grow and develop as robust plantlets. | 2004 | 15850351 |
| ginsenoside-rd from panax notoginseng enhances astrocyte differentiation from neural stem cells. | neural stem cells cultured as neurospheres were used to assess the effects of p. notoginseng on the production of neurons and glia. the crude saponins (pns) and ginsenoside-rd promote the differentiation of neurospheres into astrocytes. ginsenoside-rd increases the production of astrocytes in a dose-dependent manner. on the other hand, both pns and ginsenoside-rd induce a weak but significant effect by decreasing the number of neurons. the other ginsenosides do not induce any differentiation on ... | 2005 | 15607328 |
| impact of external calcium and calcium sensors on ginsenoside rb1 biosynthesis by panax notoginseng cells. | the effects of external calcium concentrations on biosynthesis of ginsenoside rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of panax notoginseng cells. it was observed that the synthesis of intracellular ginsenoside rb1 in 3-day incubation was dependent on the medium ca2+ concentration (0-13 mm). at an optimal ca2+ concentration of 8 mm, a maximal ginsenoside rb1 content of 1.88 +/- 0.03 mg g(-1) dry weight was reached, which was about 60% and 25% ... | 2005 | 15627250 |
| enhancement of ginsenoside biosynthesis in high-density cultivation of panax notoginseng cells by various strategies of methyl jasmonate elicitation. | a single addition of 200 microm methyl jasmonate (mja) to high-density cell cultures of panax notoginseng enhanced ginsenoside production in both shake-flask (250 ml) and airlift bioreactor (alr; 1 l working volume). repeated elicitation with two additions of 200 microm mja during cultivation further induced the ginsenoside biosynthesis in both cultivation vessels. the content of ginsenosides rg1, re, rb1 and rd in the alr was increased from, respectively, 0.18+/-0.01, 0.21+/-0.01, 0.21+/-0.02 a ... | 2005 | 15630581 |
| a dammarane glycoside derived from ginsenoside rb3. | a dammarane glycoside, designated compound mx (c-mx), was isolated from the hydrolysate of 20(s)-protopanaxadiol type ginsenosides containing g-rb(3) from panax notoginseng leaves with crude snailase. its chemical structure was elucidated to be 20-o-beta-d-xylopyranosyl(1-->6)-beta-d-glucopyranosyl-20(s)-protopanaxadiol on the basis of spectral analysis. its cytotoxicity against breast cancer cell line mcf-7 and effects on the sensitivity to doxocubicin of doxocubicin-resistant mcf-7 cells were ... | 2005 | 15684516 |
| a korean herbal medicine, panax notoginseng, prevents liver fibrosis and hepatic microvascular dysfunction in rats. | we assessed the prevention of hepatic fibrogenesis by water-extract of panax notoginseng buck f.h. chen. (arialiaceae) root (pns) in long-evans rats with cinnamon coat color (lec rats). lec rats were divided into three groups a, fed on a basal diet (bd); b, fed on bd plus 1% pns; and c), fed on bd plus 0.005% lycopene as a control. all rats were sacrificed at 26 weeks of age. the percentage of the total area involved by fibrosis was 1.46 +/- 0.47 in group a, 0.83 +/- 0.10 in b (p=0.0030, b vs a) ... | 2005 | 15698847 |
| [study on qualitative and quantitative methods for yinhuang liangxiao granule]. | to establish the quality standard of yinhuang langxiao granule. | 2004 | 15706894 |
| [correlation and path analysis of agronomic characters of panax notoginseng]. | to select the main directions and the objects in breeding the high yield of panax notoginseng by the correlation and path analysis of main agronomic character of p. notoginseng. | 2004 | 15709377 |
| [influence of compatibility ratio of fufang danshen on the dissolution of danshen compositions]. | a high performance liquid chromatographic (hplc) method has been developed using peak ratio to compare the danshen ingredients with varying peak ratios of danshen (salvia miltiorrhiza bunge.) to sanqi (panax notoginseng) from 10:0 to 1:10 in fufang danshen which is composed of danshen and sanqi. the experimental results show that the contents of major components of all samples decocted together are higher than those of danshen. the contents of danshen major components are the highest in classic ... | 2004 | 15712872 |
| [apoptosis of rat hepatic stellate cells induced by panax notoginseng saponins]. | | 2005 | 15727717 |
| structure and biological activity of protopanaxatriol-type saponins from the roots of panax notoginseng. | the further purification of the total saponins from the roots of panax notoginseng by using ordinary and reversed-phase silica-gel, as well as sephadex lh-20 chromatography afford seven adjuvant active protopanaxatriol-type saponins (pts), ginsenosides-rh1 (rh1),-rh4 (rh4),-rg1 (rg1),-re (re), notoginsenosides-r1 (r1),-r2 (r2),-u (u). these saponins were evaluated for their haemolytic activities and adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (o ... | 2006 | 16332509 |
| solid-phase extraction and liquid chromatography-electrospray mass spectrometric analysis of saponins in a chinese patent medicine of formulated salvia miltiorrhizae and panax notoginseng. | in this paper, a method using solid-phase extraction (spe) and hplc/esi-ms(n) for the identification of the major saponins in "danshen dripping pill", a chinese patent medicine consisting of salvia miltiorrhizae and panax notoginseng, is described. through solid-phase extraction process, the saponins in "danshen dripping pill" were separated from the phenolic constituents of s. miltiorrhizae and, meanwhile, efficiently concentrated. subsequently, these saponins were characterized by hplc/esi-ms( ... | 2006 | 16111853 |
| relationship between haemolytic and adjuvant activity and structure of protopanaxadiol-type saponins from the roots of panax notoginseng. | four protopanaxadiol-type saponins (pds), ginsenosides-rb(1), -rd, notoginsenosides-k, -r(4) isolated from the roots of panax notoginseng were evaluated for their haemolytic activities and adjuvant potentials on the cellular and humoral immune responses of icr mice against ovalbumin (ova). the effect of the substitution pattern of these pds on their biological activities was investigated and structure-activity relationships were established. among four pds, the ranking of the haemolytic activity ... | 2005 | 16115706 |
| [effects of the panax notoginseng saponins on the level of synaptophysin protein in brain in rat model with lesion of meynert]. | to observe the protective effect of panax notoginseng saponins (pns) on the level of synaptophysin ptotein in brain in rat model with alzheimer's disease (ad). | 2005 | 16124609 |
| [relationship between the inhibitory effects of pns on cardiac hypertrophy and its action on neurohormonal factor]. | to study the effects of total saponins of panax notoginseng (pns) on the overall model and vitro model of cardiac hypertrophy, and investigate the mechanism of its action. | 2005 | 16124610 |
| [effect of panax notoginseng saponins on intercellular adhesion molecule-1 expression and neutrophil infiltration in cerebral infarction tissue of rats]. | to investigate the effect of panax notoginseng saponins (pns) on the expression of intercellular adhesion molecule-1 (icam-1) and brain neutrophil infiltration induced by transient focal cerberal ischemia and reperfusion in rats. | 2005 | 16131033 |
| high-performance liquid chromatographic assay for the active saponins from panax notoginseng in rat tissues. | a reversed-phase liquid chromatographic method was used to determine the ginsenosides rg1, rb1 and rd of panax notoginseng in rat tissues (kidney, liver, heart, spleen and lung) after the administration of total saponins of p. notoginseng. the tissue samples were treated with solid-phase extraction prior to hplc. the calibration curves for the three saponins were linear in the given concentration ranges. the intra-day and inter-day assay coefficients in tissues were between 76 and 120% respectiv ... | 2006 | 16138297 |
| orthogonal array design in optimizing the extraction efficiency of active constituents from roots of panax notoginseng. | the root of panax notoginseng (radix notoginseng, sanqi) is a commonly used traditional chinese medicine, which is mainly cultivated in wenshan of yunnan china. the identified active constituents in radix notoginseng include saponin, ssavonoid and polysaccharide; however, the levels of these active constituents vary greatly with different extraction processes. this variation causes a serious problem in standardizing the herbal extract. by using hplc and spectrophotometry, the contents of notogin ... | 2005 | 16177971 |
| inhibitory effect of trilinolein on endothelin-1-induced c-fos gene expression in cultured neonatal rat cardiomyocytes. | trilinolein, isolated from the traditional chinese herb sanchi (panax notoginseng), has been shown to have myocardial protective effects via its antioxidant ability. however, the cellular and molecular mechanisms of the protective effect of trilinolein in the heart remain to be elucidated. oxidative mechanisms have been implicated in neonatal cardiomyocyte hypertrophy. we previously reported that et-1 induces ros generation via the et(a) receptor and ros modulates c-fos gene expression. we have ... | 2005 | 16184402 |
| determination of four active saponins of panax notoginseng in rat feces by high-performance liquid chromatography. | a method is developed for the determination of ginsenoside rg1, rb1, rd, and notoginsenoside r1 of panax notoginseng (pns) in rat feces after oral and intravenous administration of total saponins of pns. the fecal samples are treated with organic extraction and solid-phase extraction prior to high-performance liquid chromatography. the calibration curves for the four saponins are linear in the given concentration ranges. the precision of the method is in the range of 1.0-10.0% (relative standard ... | 2005 | 16212783 |
| panaxynol induces neurite outgrowth in pc12d cells via camp- and map kinase-dependent mechanisms. | panaxynol, a polyacetylene ((3r)-heptadeca-1,9-diene-4,6-diyn-3-ol; syn. falcarinol), was isolated from the lipophilic fractions of panax notoginseng, a chinese traditional medicinal plant. in the present study, we reported the neurotrophic effects of panaxynol on pc12d cells and mechanism involved in neurite outgrowth of the cells. panaxynol could morphologically promote neurite outgrowth in pc12d cells, concentration-dependently reduce cell division and up-regulate molecular marker (map1b) exp ... | 2006 | 16219303 |
| effect of bottom clearance on performance of airlift bioreactor in high-density culture of panax notoginseng cells. | a fed-batch cultivation of panax notoginseng cells in a concentric-tube airlift reactor was performed to study the effects of bottom clearance on cell growth and the production of ginseng saponin and polysaccharide. at a bottom clearance of 4.0 cm, the highest cell density of 29.1+/-1.6 g/l by dry weight was obtained, and the accumulation of saponin and polysaccharide also reached a maximum, i.e., 2.39+/-0.43 and 2.73+/-0.40 g/l, respectively. cell growth and metabolite production were limited a ... | 2001 | 16233116 |
| manipulation of ginsenoside heterogeneity in cell cultures of panax notoginseng by addition of jasmonates. | manipulation of the heterogeneity of ginsenosides with different pharmacological activities was attempted by adding methyl jasmonate (mja) or dihydro-methyl jasmonate (hmja) to suspension cultures of a high-saponin-producing cell line of panax notoginseng. compared with hmja, mja not only increased ginsenoside content to a large degree but also significantly altered the ratio of rb to rg groups of ginsenosides. the ginsenoside rd was not detected in either control cultures (without mja addition) ... | 2002 | 16233164 |
| genetic diversity and variation of saponin contents in panax notoginseng roots from a single farm. | radix notoginseng, the root of panax notoginseng (burk.) f. h. chen, has been widely used in traditional chinese medicine. its main components, saponins, have been reported to have many pharmacological activities. to test the general assumption that herbs of a single species planted and harvested from a single location are uniform in chemical and genetic makeup, chemical analysis and dna fingerprinting were carried out. high-performance tlc together with hplc analysis were used to analyze 17 ran ... | 2005 | 16248538 |
| induction and characterization of adventitious roots directly from the explants of panax notoginseng. | adventitious roots from leafstalks and lateral roots were obtained directly from explants of panax notoginseng. the lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. hplc analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside rd was missing, compared with the saponins extracted from the raw herbs. the dry weight of primary adventitious root culture of pana ... | 2005 | 16314969 |
| simultaneous determination of nine saponins from panax notoginseng using hplc and pressurized liquid extraction. | a hplc and pressurized liquid extraction (ple) method was developed for simultaneous determination of nine saponins, including notoginsenoside r1, ginsenoside rg1, re, rf, rb1, rc, rb2, rb3 and rd in panax notoginseng. the analysis was performed on c18 column with water-acetonitrile gradient elution and the investigated saponins were authenticated by comparing retention time and mass spectra with their reference compounds. several methods including ple, ultrasonication, soxhlet extraction and im ... | 2006 | 16316736 |
| characterization of cell wall polysaccharides from the medicinal plant panax notoginseng. | panax notoginseng is a commonly used medicinal plant in south-western china. recent studies indicate that wall polysaccharides are responsible for some of the immunostimulatory activity. fractionation of the p. notoginseng root powder alcohol insoluble residue (air) and its compositional analysis enabled us to deduce the polysaccharide and protein composition of the root cell walls. p. notoginseng walls are composed primarily of polysaccharide (approximately 97% w/w) and some protein. the polysa ... | 2005 | 15896377 |
| simultaneous quantification of six major active saponins of panax notoginseng by high-performance liquid chromatography-uv method. | a simple, sensitive and specific high-performance liquid chromatography-uv (hplc-uv) method has been developed for the first time to simultaneously quantify the six major active saponins of panax notoginseng, namely notoginsenoside r1, ginsenoside rg1, rb1, rg2, rh1 and rd. astragaloside iv is used as the internal standard. this hplc assay was performed on a reversed-phase c18 column with gradient elution of acetonitrile and 0.01% formic acid in 30 min. the method provided good reproducibility a ... | 2005 | 15907618 |
| panax notoginseng saponins preconditioning protects rat liver grafts from ischemia/reperfusion injury via an antiapoptotic pathway. | ischemia/reperfusion (i/r) injury is a major cause of primary graft dysfunction and renders an allograft more immunogenic in orthotopic liver transplantation (olt). panax notoginseng saponins (pns) has been reported to exert protective effects against i/r injury to various organs. the objective of this study is to investigate whether pns preconditioning protects rat liver grafts from i/r injury via an antiapoptotic pathway. | 2005 | 15908317 |
| [effects of panax notoginseng saponins on the process of renal interstitial fibrosis after unilateral ureteral obstruction in rats]. | to evaluate the effects of panax notoginseng saponins (pns) on the process of renal interstitial fibrosis after unilateral ureteral obstruction in rats. | 2005 | 15931871 |
| up-regulation of m1 muscarinic receptors expressed in chom1 cells by panaxynol via camp pathway. | loss of cholinergic neurons along with muscarinic acetylcholine receptors (machrs) in cerebral cortex and hippocampus is closely associated with alzheimer's disease (ad). recent drug development for ad treatment focuses heavily on identifying m(1) receptor agonists. however, machrs undergo down-regulation in response to agonist-induced sustained activation. therefore, therapeutic effectiveness wanes during continuous use. thus, another potentially effective approach, which overcomes this drawbac ... | 2005 | 15936523 |
| molecular mechanism of the inhibitory effect of trilinolein on endothelin-1-induced hypertrophy of cultured neonatal rat cardiomyocytes. | trilinolein, isolated from the traditional chinese herb sanchi ( panax notoginseng), has been shown to have myocardial protective effects via its antioxidant ability. however, the cellular and molecular mechanisms of the protective effect of trilinolein in the heart remain to be elucidated. oxidative mechanisms have been implicated in neonatal cardiomyocyte hypertrophy. we therefore have examined whether trilinolein attenuates reactive oxygen species (ros) production and thus et-1-induced hypert ... | 2005 | 15971123 |
| [protective effects of panax notoginseng saponins against pathological lesion of cholinergic neuron in rat model with alzheimer' s disease]. | to observe the protective effect of panax notoginseng saponins (pns) against pathological lesion of cholinergic neuron in rat model with alzheimer' s disease (ad). | 2005 | 15981887 |
| [the pharmacokinetics and pharmacodynamics of intranasal preparation of panax notoginseng saponins]. | to investigate the pharmacokinetic course of intranasal powders of panax notoginseng saponins (pns) in a rat model and its protective effects against cardio-cerebrovascular diseases administrated in the form of its suspension. | 2005 | 16011272 |
| [effects of ethanol extracts of panax notoginseng on liver metastasis of b16 melanoma grafted in mice]. | to observe the effects of ethanol extracts of panax notoginseng on the tumor and the liver metastasis in experimental mice grafted with b16 melanoma. | 2006 | 16965745 |
| efficient induction of ginsenoside biosynthesis and alteration of ginsenoside heterogeneity in cell cultures of panax notoginseng by using chemically synthesized 2-hydroxyethyl jasmonate. | chemically synthesized 2-hydroxyethyl jasmonate (heja) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. the dose response and timing of heja elicitation were investigated in cell suspension cultures of panax notoginseng. the optimal concentration and timing of heja addition for both cell growth and ginsenoside accumulation was identified to be 200 microm added on day 4. it was interestingly found that heja ... | 2006 | 16041570 |