| leptospirosis associated with serovars hardjo and pomona in red deer calves (cervus elaphus). | four red deer calves (cervus elaphus) died with severe nephritis apparently associated with infection by leptospira interrogans serovar pomona. the sera of 12 in-contact red deer calves were examined for leptospiral agglutinins and nine showed titres to pomona consistent with recent infection. two also showed titres of 1:100 to serovar hardjo. the urine of five of these in-contact calves was examined periodically over a period of nine months. all were initially leptospiruric, four being infected ... | 1984 | 16031052 |
| haemolytic disease associated with leptospira interrogans serovar pomona in red deer calves (cervus elaphus). | three red deer calves (cervus elaphus) died with a haemolytic disease associated with infection by leptospira interrogans serovar pomona. infection within the herd was more prevalent than disease. sera from 16 herd mates were tested by the microscopic agglutination test (mat) and 12 had leptospiral titres, the majority to serovar pomona. a few calves had titres to balcunica and hardjo. urine was obtained for culture from six of these calves and serovar pomona was isolated from five with titres t ... | 1986 | 16031298 |
| the epidemiology of leptospira interrogans serovar pomona in grower pig herds. | an epidemiological study on four farms showed that leptospiral infection became apparent in piglets from 12 weeks of age. the intensity of leptospiruria was greatest in the first 3-4 weeks of infection and from then on declined and became intermittent. factors affecting the cultural and serological prevalence of infection of the piglets were found to be the standard of hygiene and variations in the titre of the dam. dams with high titres gave better protection to their young than did those with ... | 1995 | 16031793 |
| geographical dissemination of leptospira interrogans serovar pomona during seasonal migration of california sea lions. | leptospirosis is one of the most widespread bacterial zoonoses in the world and affects most mammalian species. although leptospirosis is well documented and characterized in terrestrial species, less information is available regarding the distribution and impact of leptospirosis in marine mammals. additionally, the role of animal migrations on the geographical spread of leptospirosis has not been reported. periodic epizootic outbreaks of acute leptospirosis among california sea lions (zalophus ... | 2009 | 19186009 |
| are white-spot lesions in kidneys in sheep associated with leptospirosis? | to determine the association between white-spot lesions in kidneys and serological and cultural prevalence of leptospirosis in sheep, and to evaluate the diagnostic value of these lesions in individual sheep and lines of sheep at slaughter as indicators of past or current episodes of leptospirosis. | 2009 | 19252540 |
| differential response of cytokines induced by leptospira interrogans, serogroup pomona, serovar pomona, in mouse and human cell lines. | leptospira interrogans, the causative agent of leptospirosis, is an important zoonotic bacterium. the mechanisms and roles of cytokine induction in both humans and animals remain unclear. therefore, the ifn-gamma, il-6, il-10 and il-12 levels were measured by enzyme-linked immunosorbent assay (elisa) in human thp-1 and mouse raw 264.7 monocyte cell lines following stimulation with heat-killed leptospira interrogans serogroup pomona serovar pomona, l. biflexa, e. coli or salmonella group b. the p ... | 2008 | 19317342 |
| proteome analysis of leptospira interrogans virulent strain. | leptospirosis is a worldwide zoonotic infection of human and veterinary concern. caused by pathogenic spirochetes of the genus leptospira, the disease presents greater incidence in tropical and subtropical regions. the identification of proteins that could be involved in the bacteria host interactions may facilitate the search for immune protective antigens. we report the proteomic analysis of leptospira interrogans serovar pomona virulent strain lpf cultured from kidney and liver of infected ha ... | 2009 | 19590580 |
| a serological survey of leptospirosis in cattle of rural communities in the province of kwazulu-natal, south africa. | a serological survey of leptospirosis in cattle originating from rural communities of the province of kwazulu-natal (kzn) in south africa was carried out between march 2001 and december 2003. the survey was designed as a 2-stage survey, using the local diptank as the primary sampling point. in total, 2021 animals from 379 diptanks in 33 magisterial districts were sampled and tested with the microscopic agglutination test (mat). the apparent prevalence at district level was adjusted for clusterin ... | 2009 | 19653519 |
| immune response and prophylactic efficacy of smegmosomes in a hamster model of leptospirosis. | leptospirosis is an important zoonotic disease worldwide. subunit vaccines are an attractive intervention strategy against this disease, but potent, non-toxic adjuvants are necessary components to any effective vaccine. among various adjuvant candidates, liposomes have garnered recent attention for their capacity as carriers of vaccines. in the present study we prepared novel liposomes using total polar lipids from the nonpathogenic bacterium, mycobacterium smegmatis (designated smegmosomes). th ... | 2009 | 19715780 |
| leptosome-entrapped leptospiral antigens conferred significant higher levels of protection than those entrapped with pc-liposomes in a hamster model. | we prepared novel liposomes from total polar lipids of non-pathogenic leptospira biflexa serovar potac (designated leptosomes) and evaluated their vaccine delivery/adjuvant potential with novel protective antigens (lp0607, lp1118 and lp1454) of l. interrogans serovar pomona in a hamster model. the immune response induced by three individual antigens and protective efficacy were evaluated and compared to those induced by same antigens entrapped with pc-liposomes and e. coli lipid liposomes (esche ... | 2009 | 19729088 |
| identification and characterization of ompa-like proteins as novel vaccine candidates for leptospirosis. | leptospira is an important infectious gram-negative bacterium causing leptospirosis in mammals. outer membrane proteins (omps) are key molecules in the interface between the cell and its environment. a group of putative leptospiral outer membrane proteins with an ompa-like domain, comprising lp0056, lp0222, lp3615, lp3685, lp4337 and lbp328, were identified by bioinformatic methods and expressed as gst-tag fusion proteins. all these recombinant proteins were screened for immune-protective potent ... | 2010 | 20064477 |
| cytopathic effect in bhk 21 (c13) cells inoculated with leptospira interrogans serovar pomona isolated from a porcine abortion. | | 2009 | 20085192 |
| [ultramicroscopic alterations in placental tissue from infected animals with leptospira interrogans serovar pomona]. | | 2011 | 21491071 |
| serosurvey of leptospirosis and investigation of a possible novel serovar arborea in farmed deer in new zealand. | to investigate the prevalence of leptospira spp. and possible novel serovar arborea infection in farmed deer in new zealand. | 2011 | 21541888 |
| growth response and shedding of leptospira spp. in urine following vaccination for leptospirosis in young farmed deer. | abstract | 2012 | 22175424 |
| immunoprotection of recombinant leptospiral immunoglobulin-like protein a against leptospira interrogans serovar pomona infection. | we previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins liga and ligb of leptospira interrogans. liga and ligb are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. here, we evaluate the potential of recombinant liga (rliga) as a vaccine candidate against infection by l. interrogans serovar pomona in a hamster model. rliga was truncated into conserved (rligacon) and variable (rligavar) regions and expressed in esc ... | 2006 | 16495547 |
| [immunogenicity and protective capacity of leptospiral whole-cell monovalent serogroup ballum vaccines in hamsters]. | leptospira serogroup ballum is at present the first cause of human leptospirosis in cuba. killed whole-cell vaccines were formulated with two clinical isolates of leptospira interrogans serogroup ballum using aluminum hydroxide as adjuvant. agglutinins levels induced by each vaccine in hamsters were estimated by microscopic agglutination test and specific igg activities were quantified by a whole cell-based enzyme-linked immunosorbent assay. homologous and cross protective capacity against letha ... | 2005 | 16502635 |
| multiple-locus variable-number tandem repeat analysis (mlva) of leptospira interrogans serovar pomona from argentina reveals four new genotypes. | outbreaks of leptospirosis occur regularly in argentina, but little is known about their epidemiological relationships. we have analyzed the genetic diversity of a collection of 16 strains of leptospira interrogans serovar pomona isolated from animals and humans in argentina during the past 45 years. genotyping was performed by multiple-locus variable-number tandem repeat analysis (mlva) using the loci vntr4, vntr7, vntr9, vntr10, vntr19, vntr23 and vntr31, as described by majed et al. [identifi ... | 2008 | 17531318 |
| leptospirosis in farmed deer in new zealand : a review. | current knowledge of leptospirosis in farmed deer in new zealand is reviewed. over the past 25 years, leptospirosis has been reported to occur in individual cases as well as in herd outbreaks in farmed deer and in human cases linked to farmed deer. serological studies and evidence from bacterial culture suggest infection is widespread. mixing of young stock from several sources appears to be a significant risk factor for outbreaks. the culture of leptospira interrogans serovars hardjobovis, pomo ... | 2007 | 17534410 |
| [development of a method to determine the minimum inhibitory concentration in reference strains from leptospiras]. | a method to determine the minimum inhibitory concentration for leptospiras was developed, since there is not a standard method to measure it at the international level. reference strains from the pathogenic complex l. interrogans and l. biflexa were used against penicillin, cyprofloxacine, chloramphenicol, rifampicine and tetracycline. the minimum inhibitory concentration was defined as the lowest concentration of antibiotic where it was observed the inhibition of the bacterial mobility by direc ... | 2005 | 17966469 |
| comparison of different dna extraction and polymerase chain reaction methods to detect leptospira spp. on field samples. | it was carried out a comparison on two reference leptospira strains dna between different extraction methods and two polymerase chain reaction protocol. the dna was quantified and serial dilutions were tested by polymerase chain reaction. the results showed difference in terms of recovery and sensitivity between this methods. | 2005 | 17966479 |
| [seropositivity to leptospiras in sera from patients suspected of viral hepatitis negative to serological markers]. | the prevalence of leptospirosis found in icteric patients negative to serological markers for hepatitis was described. 15.99 % of them were positive patients. it was observed a predominance of serovars canicola, pomona and icterohaemorrhagiae. | 2005 | 17966482 |
| [evaluation of the vaccine potentiality of 2 strains from leptospira interrogans serogroup ballum]. | two vacine candidate strains from ballum serogroup were characterized and their immunogenicity and protective power were studied in hamster. the monovalent vaccine formulations obtained showed a high immunogenicity and capacity of homologous protection and, in a lesser degree, of cross protection. | 2005 | 17966485 |
| immunogenicity and protective efficacy of recombinant leptospira immunoglobulin-like protein b (rligb) in a hamster challenge model. | leptospiral immunoglobulin-like protein (ligb) was truncated into conserved (ligbcon) and variable (varb1, varb2) fragments and expressed as gst/his-tag fusion proteins. four-week-old hamsters were immunized with equal amounts of each fragment individually or combined in alum adjuvant at days 0 and 21 and subsequently challenged three weeks after the booster with 2.5 ld(50) live virulent leptospira interrogans serovar pomona. our results demonstrate that immunization with ligb produced strong hu ... | 2009 | 19070678 |
| expression of major histocompatibility complex class ii antigens in porcine leptospiral nephritis. | class ii major histocompatibility complex (mhcii) is required for the presentation of antigens to cd4 helper t cells. during nephritis, not only primary antigen presenting cells such as histiocytes and lymphocytes, but also cytokine-stimulated tubular epithelial cells express mhcii. leptospirosis in fattening pigs is characterized by several degrees of nephritis, from absence of lesions to severe multifocal tubulo-interstitial inflammation. renal tissue from 20 8-month-old pigs with spontaneous ... | 2009 | 19179617 |