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a pediatric case of anaphylaxis caused by matsutake mushroom (tricholoma matsutake) ingestion.anaphylaxis is one of the severest forms of allergic diseases. some kinds of mushroom are known as causative allergens in food anaphylaxis. matsutake mushroom (tricholoma matsutake) is a typical edible mushroom available in autumn in japan. we encountered an 8-year-old japanese girl who developed anaphylaxis after ingesting matsutake mushrooms.200617075291
metabolomic discrimination of different grades of pine-mushroom (tricholoma matsutake sing.) using 1h nmr spectrometry and multivariate data analysis.metabolomic analysis of raw and cooked pine-mushrooms (tricholoma matsutake sing.) of different grades was performed using (1)h nuclear magnetic resonance (nmr) spectrometry and principal component analysis (pca). pca of the (1)h nmr spectra of aqueous fractions allowed different grades of raw pine-mushroom to be discriminated by a combination of principal component (pc) 1 and pc 2, which accounted cumulatively for 94.1% of the variation in all variables. the major peaks in the (1)h nmr spectra ...200717029671
characterization of aroma-active compounds in raw and cooked pine-mushrooms (tricholoma matsutake sing.).the characteristic aroma-active compounds in raw and cooked pine-mushrooms (tricholoma matsutake sing.) were investigated by gas chromatography-olfactometry using aroma extract dilution analysis. 1-octen-3-one (mushroom-like) was the major aroma-active compound in raw pine-mushrooms; this compound had the highest flavor dilution factor, followed by ethyl 2-methylbutyrate (floral and sweet), linalool (citrus-like), methional (boiled potato-like), 3-octanol (mushroom-like and buttery), 1-octen-3-o ...200616910727
difference in the volatile composition of pine-mushrooms (tricholoma matsutake sing.) according to their grades.the differences in volatile components of pine-mushrooms (tricholoma matsutake sing.) according to their grades were observed by applying multivariate statistical methods to gc-ms data sets. a total of 35 and 37 volatile components were identified in raw and cooked pine-mushrooms, respectively. the volatile components in pine-mushrooms were primarily composed of c8 species, such as 3-octanol, 1-octen-3-ol, 1-octanol, (e)-2-octen-1-ol, 3-octanone, 1-octen-3-one, (e)-2-octenal, and octanoic acid. ...200616787034
submerged culture of tricholoma matsutake mycelium in bubble column fermentors.submerged culture of tricholoma matsutake mycelium was carried out using two bubble column fermentors, a standard bubble column and an external-loop airlift column. the effects of the aeration rate and column type on culture performance in terms of the mycelia morphology, glucose consumption, cell yield, and growth rate were investigated. morphologically, three types of pellets-large spherical, small spherical and filamentous-were observed depending on the aeration rate. on the whole, the standa ...199916232437
stereochemical correlation between 10-hydroperoxyoctadecadienoic acid and 1-octen-3-ol in lentinula edodes and tricholoma matsutake mushrooms.linoleic acid (la) incubated with a homogenate of lentinula edodes or tricholoma matsutake mushroom significantly increased the amount of (r)-1-octen-3-ol. the alcohol was identified as (s)-10-hode with 90-87% and >99% enantiomeric excess (ee), respectively. during the incubation of la with these homogenates in the presence of glutathione-glutathione peroxidase (gsh-gpx), which can reduce hydroperoxy fatty acids to the corresponding hydroxy acids, the formation of (r)-1-octen-3-ol was significan ...200516116283
intra- and inter-specific variations in the copy number of two types of retrotransposons from the ectomycorrhizal basidiomycete tricholoma matsutake.to explore intra- and inter-specific variations of the ectomycorrhizal basidiomycete tricholoma matsutake that produces the fruit body "matsutake", we carried out real-time pcr analysis based on two types of retrotransposons, one designated mary1, which resembles a retrovirus carrying the long terminal repeat (ltr) and the other mary2n, which resembles mrna carrying the polyadenylated tail. calculation based on the average genome size of homobasidiomycetes (34 mbp) shows that ca. 5.5% of the tot ...200515940467
growth promotion of mycelia of the matsutake mushroom tricholoma matsutake by d-isoleucine.mycelial growth of the matsutake mushroom (tricholama matsutake) was much slower than that of the other mushroom species. we found that the addition of d-isoleucine to the culture medium strikingly promoted mycelia growth. the other amino acids tested had no effect on this growth promotion.200415564685
phylogeography and evolution in matsutake and close allies inferred by analyses of its sequences and aflps.matsutake are commercially important ectomycorrhizal basidiomycetes in the genus tricholoma. despite their importance, the systematics of this species complex have remained elusive and little is known about their origin and biogeography. using dna analyses on a worldwide sample of matsutake, we present here the first comprehensive definition of natural groupings in this species complex. we infer patterns of migration and propose eocene origins for the group in western north america by a transfer ...201021148894
sigma mary1, the ltr of the gypsy-type retroelement mary1 from the basidiomycete tricholoma matsutake, allows multicopy dna integration in lentinula edodes.sigma mary1 is the ltr of the retroelement mary1 from the homobasidiomycete tricholoma matsutake. upon integration through transformation, plc1-hph carrying a sigma mary1 derivative, sigma* mary1, conferred the hygromycin-resistant phenotype stronger than the vector without sigma* mary1 on lentinula edodes. based on the densitometric analysis after southern hybridization, a copy number of the former construct integrated in the genome is much higher than that of the latter. we conclude that sigma ...200414745187
inhibition of decrease in natural killer cell activity in repeatedly restraint-stressed mice by a biological response modifier derived from cultured mycelia of the basidiomycete tricholoma matsutake.to develop a method to cope with stress-induced reduction in immunocompetence, we evaluated the immunomodulatory activities of a biological response modifier derived from the mycelia of the basidiomycete tricholoma matsutake (cm6271) in mice under repeated restraint stress.200414557678
enhanced recovery of nk cell activity in mice under restraint stress by the administration of a biological response modifier derived from the mycelia of the basidiomycete tricholoma matsutake.some types of stressor act on the immune system via the network comprising the endocrine-immune-nervous systems, and are reportedly responsible for the onset of diseases as well as giving impetus to their advance. it is important for the maintenance and promotion of health to cope with stress-induced changes in immunocompetence. therefore, we studied the effects of administration of a novel biological response modifier (cm6271) derived from the mycelia of the basidiomycete tricholoma matsutake o ...200312775334
[a case of anaphylaxis due to matsutake mushroom]. 200312754421
genetic evidence that two types of retroelements evolved through different pathways in ectomycorrhizal homobasidiomycetes tricholoma spp.we designed a polymerase chain reaction that allows us to clone two types of putative reverse transcriptase genes from 11 species of ectomycorrhizal basidiomycetes that belong to the genus tricholoma. one corresponds to the putative gene of mary1, a long terminal repeat (ltr) retroelement from tricholoma matsutake, and the other, mary2n, a line-like non-ltr (l1-like) retroelement from this fungus. putative protein products predicted from nucleotide sequencing of cloned fragments were phylogeneti ...200212400687
mary2n, a line-like non-long terminal repeat (non-ltr) retroelement from the ectomycorrhizal homobasidiomycete tricholoma matsutake.a line-like non-ltr retroelement designated mary2n was cloned from the ectomycorrhizal homobasidiomycete tricholoma matsutake. mary2n has open reading frames that correspond to gag and pol, and a putative promoter and consensus sequences common to those of the mutators from fruit flies. while it is common to t. matsutake and tricholoma magnivelare, mary2n does not reside in any other species of tricholoma tested.200111758927
the long terminal repeat (ltr) sequence of mary1, a retroelement from the ectomycorrhizal homobasidiomycete tricholoma matsutake, is highly conserved in various higher fungi.mary1 is an ltr-retroelement from the homobasidiomycete tricholoma matsutake. nucleotide sequences that correspond to the putative u3-r region and the r-u5 region of mary1 are highly conserved in various higher fungi. data suggest that the ltr sequence of mary1 originated early in the evolution of higher fungi and has become widely distributed. therefore, it may be useful for the construction of an ltr-mediated transformation system in basidiomycetes.200111758926
mary1, a member of the gypsy group of long terminal repeat retroelements from the ectomycorrhizal basidiomycete tricholoma matsutake.we cloned an intact copy of a long terminal repeat retroelement designated mary1 from the ectomycorrhizal basidiomycete tricholoma matsutake. the reverse transcriptase domain is found in t. matsutake and tricholoma magnivelare worldwide. this finding suggests that retroelements associate with ectomycorrhizal basidiomycetes and may be useful as genetic markers for identification, phylogenetic analysis, and mutagenesis of this fungal group.200010919835
sterol constituents from two edible mushrooms, lentinula edodes and tricholoma matsutake.two new sterols, (22e)-23-methylergosta-5,7,22-trien-beta-ol (1) and 5alpha,6alpha-epoxy-(22e)-ergosta-8,14,22-triene -3beta,7alpha-diol (2), have been isolated from two edible mushrooms, lentinula edodes and tricholoma matsutake, respectively, together with twelve known ones (3-14). the structures of the new compounds were elucidated on the basis of their spectral data.200010823718
acute renal failure associated with suspected amanita smithiana mushroom ingestions: a case series.mushroom poisoning leading to acute renal failure is extremely rare in north america. cortinarius species and amanita smithiana both can cause acute renal failure and inhabit the pacific northwest. this article describes 4 patients who presented in acute renal failure and who ingested mushrooms described as resembling a. smithiana. two patients (a 74-year-old korean couple) described eating mushrooms with an approximately 6 x 0.5-inch stipe with a white cap, 1.25-1.5 inches in diameter. the othe ...19989715243
small-subunit ribosomal dna of an ectomycorrhizal fungus tricholoma matsutake: sequence, structure and phylogenetic analysis.a 2861 bp nucleotide sequence containing tricholoma matsutake ssu rrna gene and its flanking regions was determined and analyzed. comparison with known ssu rdna sequences and primer extension analysis revealed that the ssu rrna coding region and intergenic spacer 2 (igs2) are 1805 bp and 1043 bp in length, respectively. the igs2 has an imperfect direct repeat (type 1) homologous to the region downstream to the 5s rrna gene and the three imperfect tandem direct repeats (type 2) upstream to the ss ...19989666460
food-dependent exercise-induced anaphylaxis due to matsutake mushrooms. 19979205535
renal failure caused by mushroom poisoning.in the pacific northwest region of the us and in southwestern british columbia, canada, isolated cases of renal failure have occurred following ingestion of wild mushrooms. we report four cases in which toxic mushrooms were mistaken for the edible pine mushroom or matsutake (tricholoma magnivelare).19979022655
local-level genetic diversity and structure of matsutake mushroom (tricholoma matsutake) populations in nagano prefecture, japan, revealed by 15 microsatellite markers.the annual yield of matsutake mushrooms (tricholoma matsutake) has consistently decreased in japan over the past few decades. we used 15 polymorphic and codominant simple sequence repeat (ssr) markers, developed using next-generation sequencing, to carry out genetic analyses of 10 populations in nagano, japan. using the ssrs, we identified 223 genotypes, none of which was observed in more than one population. the mean expected heterozygosity and standardized allelic richness values were 0.67 and ...201729371541
ectomycorrhization of tricholoma matsutake with quercus aquifolioides affects the endophytic microbial community of host plant.tricholoma matsutake (s. ito et imai) is an ectomycorrhizal basidiomycete associated with pinaceae and fagaceae trees in the northern hemisphere. it is still unknown whether the symbiotic relationship with this ectomycorrhiza could affect the host plant's endophytic microbial community. in this study, we used high throughput sequencing to analyze the endophytic microbial communities of different quercus aquifolioides tissues with or without t. matsutake partner. about 35,000 clean reads were obt ...201829359810
applied mycology can contribute to sustainable rural livelihoods: building upon china's matsutake management initiatives.matsutake mushrooms are an important part of rural livelihoods and forest ecosystems across large parts of china, as well as elsewhere in east asia, northern europe and north america. mushroom harvesters have developed sophisticated understandings of matsutake ecology and production, and are applying this knowledge in various innovative management strategies. at the same time, chinese government agencies and scientists are promoting matsutake-based livelihoods to support development and conserva ...201729273995
heavy-ion beam mutagenesis of the ectomycorrhizal agaricomycete tricholoma matsutake that produces the prized mushroom "matsutake" in conifer forests.tricholoma matsutake is an ectomycorrhizal agaricomycete that produces the prized mushroom "matsutake" in pinaceae forests. currently, there are no available cultivars or cultivation methods that produce fruiting bodies. heavy-ion beams, which induce mutations through double-stranded dna breaks, have been used widely for plant breeding. in the present study, we examined whether heavy-ion beams could be useful in isolating t. matsutake mutants. an argon-ion beam gave a suitable lethality curve in ...201829164316
artificial 137cs and natural 40k in mushrooms from the subalpine region of the minya konka summit and yunnan province in china.a study on 137cs pollution and activity concentrations of 40k in mushrooms of the genera cortinarius, leccinum, russula, tricholoma, tylopilus, and xerocomus from two neighboring regions in southwest china in 2010-2013 revealed different patterns of pollution with 137cs, which seemed to be highly dependent on climate conditions. tricholoma matsutake was collected in yunnan before and after the fukushima dai-ichi nuclear accident and showed similar contamination with 137cs. mushrooms from the ele ...201829052148
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