| crude extracts of marine-derived and soil fungi of the genus neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines. | the crude ethyl acetate extracts of marine-derived fungi neosartorya tsunodae kufc 9213 (e1) and n. laciniosa kufc 7896 (e2), and soil fungus n. fischeri kufc 6344 (e3) were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines. | 2017 | 26941530 |
| comparison of chemical sensitivity of fresh and long-stored heat resistant neosartorya fischeri environmental isolates using biolog phenotype microarray system. | spoilage of heat processed food and beverage by heat resistant fungi (hrf) is a major problem for food industry in many countries. neosartorya fischeri is the leading source of spoilage in thermally processed products. its resistance to heat processing and toxigenicity makes studies about neosartorya fischeri metabolism and chemical sensitivity essential. in this study chemical sensitivity of two environmental neosartorya fischeri isolates were compared. one was isolated from canned apples in 19 ... | 2016 | 26815302 |
| hydrophilins in the filamentous fungus neosartorya fischeri (aspergillus fischeri) have protective activity against several types of microbial water stress. | hydrophilins are proteins that occur in all domains of life and protect cells and organisms against drought and other stresses. they include most of the late embryogenesis abundant (lea) proteins and the heat shock protein (hsp) hsp12. here, the role of a predicted lea-like protein (leama) and two hsp12 proteins (hsp12a and hsp12b) of neosartorya fischeri was studied. this filamentous fungus forms ascospores that belong to the most stress-resistant eukaryotic cells described to date. heterologou ... | 2016 | 26487515 |
| microarray analysis of neosartorya fischeri using different carbon sources, petroleum asphaltenes and glucose-peptone. | asphaltenes are considered as the most recalcitrant petroleum fraction and represent a big problem for the recovery, separation and processing of heavy oils and bitumens. neosartorya fischeri is a saprophytic fungus that is able to grow using asphaltenes as the sole carbon source [1]. we performed transcription profiling using a custom designed microarray with the complete genome from n. fischeri nrrl 181 in order to identify genes related to the transformation of asphaltenes [1]. data analysis ... | 2015 | 26484261 |
| a novel gh7 endo-β-1,4-glucanase from neosartorya fischeri p1 with good thermostability, broad substrate specificity and potential application in the brewing industry. | an endo-β-1,4-glucanase gene, cel7a, was cloned from the thermophilic cellulase-producing fungus neosartorya fischeri p1 and expressed in pichia pastoris. the 1,410-bp full-length gene encodes a polypeptide of 469 amino acids consisting of a putative signal peptide at residues 1-20, a catalytic domain of glycoside hydrolase family 7 (gh7), a short thr/ser-rich linker and a family 1 carbohydrate-binding module (cbm 1). the purified recombinant cel7a had ph and temperature optima of ph 5.0 and 60° ... | 2015 | 26360701 |
| insight into the antifungal mechanism of neosartorya fischeri antifungal protein. | small, cysteine-rich, highly stable antifungal proteins secreted by filamentous ascomycetes have great potential for the development of novel antifungal strategies. however, their practical application is still limited due to their not fully clarified mode of action. the aim of this work was to provide a deep insight into the antifungal mechanism of neosartorya fischeri antifungal protein (nfap), a novel representative of this protein group. within a short exposure time to nfap, reduced cellular ... | 2015 | 25994413 |
| structural analysis of novel trehalose-based oligosaccharides from extremely stress-tolerant ascospores of neosartorya fischeri (aspergillus fischeri). | different fungi, including the genera neosartorya, byssochlamys and talaromyces, produce (asco)spores that survive pasteurization treatments and are regarded as the most stress-resistant eukaryotic cells. here, the nmr analysis of a series of trehalose-based oligosaccharides, being compatible solutes that are accumulated to high levels in ascospores of the fungus neosartorya fischeri, is presented. these oligosaccharides consist of an α,α-trehalose backbone, extended with one [α-d-glcp-(1 → 6)-α ... | 2015 | 25974853 |
| the first case of feline sinonasal aspergillosis due to aspergillus fischeri in japan. | feline upper respiratory tract infection due to aspergillus spp. is considered an emerging disease, with the number of reported cases continuing to rise. in this study, we report the first case of feline sinonasal aspergillosis caused by aspergillus fischeri in japan. the patient presented after 2 months of progressive facial deformity around the nose and nasal discharge. the isolate from this case was susceptible to itraconazole (itz), voriconazole and micafungin, but was resistant to amphoteri ... | 2015 | 25947561 |
| targeted production of secondary metabolites by coexpression of non-ribosomal peptide synthetase and prenyltransferase genes in aspergillus. | prenylated tryptophan-containing cyclic dipeptides are found in different fungi and serve as precursors for the biosynthesis of diverse biologically active secondary metabolites. they show distinct and usually higher biological and pharmacological activities than the respective non-prenylated dipeptides. successful production of such compounds were achieved by a new approach based on the coexpression of ftmps, a non-ribosomal peptide synthetase from neosartorya fischeri, with three cyclic dipept ... | 2015 | 25744649 |
| sartorypyrone d: a new nadh-fumarate reductase inhibitor produced by neosartorya fischeri fo-5897. | | 2015 | 25586023 |
| erratum to: insights into the substrate specificity and synergy with mannanase of family 27 α-galactosidases from neosartorya fischeri p1. | | 2015 | 25573475 |
| discovery and characterization of a group of fungal polycyclic polyketide prenyltransferases. | the prenyltransferase (ptase) gene vrtc was proposed to be involved in viridicatumtoxin (1) biosynthesis in penicillium aethiopicum. targeted gene deletion and reconstitution of recombinant vrtc activity in vitro established that vrtc is a geranyl transferase that catalyzes a regiospecific friedel-crafts alkylation of the naphthacenedione carboxamide intermediate 2 at carbon 6 with geranyl diphosphate. vrtc can function in the absence of divalent ions and can utilize similar naphthacenedione sub ... | 2012 | 22590971 |
| biochemical characterization of a gh53 endo-β-1,4-galactanase and a gh35 exo-β-1,4-galactanase from penicillium chrysogenum. | an endo-β-1,4-galactanase (pcgal1) and an exo-β-1,4-galactanase (pcgalx35c) were purified from the culture filtrate of penicillium chrysogenum 31b. pcgal1 and pcgalx35c cdnas encoding pcgal1 and pcgalx35c were isolated by in vitro cloning. the deduced amino acid sequences of pcgal1 and pcgalx35c are highly similar to a putative endo-β-1,4-galactanase of aspergillus terreus (70% amino acid identity) and a putative β-galactosidase of neosartorya fischeri (72%), respectively. pfam analysis revealed ... | 2013 | 22584433 |
| nfap2, a novel cysteine-rich anti-yeast protein from neosartorya fischeri nrrl 181: isolation and characterization. | the increasing incidence of fungal infections and damages due to drug-resistant fungi urges the development of new antifungal strategies. the cysteine-rich antifungal proteins from filamentous ascomycetes provide a feasible base for protection against molds due to their potent antifungal activity on them. in contrast to this, they show no or weak activity on yeasts, hence their applicability against this group of fungi is questionable. in the present study a 5.6 kda anti-yeast protein (nfap2) is ... | 2016 | 27637945 |
| avenaciolides: potential mura-targeted inhibitors against peptidoglycan biosynthesis in methicillin-resistant staphylococcus aureus (mrsa). | discovery of new antibiotics for combating methicillin-resistant staphylococcus aureus (mrsa) is of vital importance in the post-antibiotic era. here, we report four avenaciolide derivatives (1-4) isolated from neosartorya fischeri, three of which had significant antimicrobial activity against mrsa. the morphology of avenaciolide-treated cells was protoplast-like, which indicated that cell wall biosynthesis was interrupted. comparing the structures and minimum inhibitory concentrations of 1-4, t ... | 2015 | 25521652 |
| insights into the substrate specificity and synergy with mannanase of family 27 α-galactosidases from neosartorya fischeri p1. | thermophilic neosartorya fischeri p1 is an excellent carbohydrate-active enzyme (cazyme) producer. two α-galactosidases of gh (glycoside hydrolase ) family 27 with a very low sequence identity (28.7%), gal27a and gal27b, were identified in strain p1 and functionally expressed in pichia pastoris. in comparison to other characterized gh27 fungal counterparts, rgal27b has a higher temperature optimum (75 °c) and better thermostability (>50% activity at 70 °c for 15 min), and rgal27a shows stability ... | 2015 | 25487894 |
| diversity and specificity of microsatellites within aspergillus section fumigati. | microsatellites (or short tandem repeats, strs) are the genetic markers of choice for studying aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. however, the specificity of these markers must be investigated in a group of isolates from closely related species. the aim of this work was to test a microsatellite-based pcr multiplex previously designed for a. fumigatus in a set of species belonging to section fumigati, namely aspergillus fumigatia ... | 2012 | 22838495 |
| a thermophilic β-mannanase from neosartorya fischeri p1 with broad ph stability and significant hydrolysis ability of various mannan polymers. | a new β-mannanase gene, man5p1, was cloned from the thermophilic fungus neosartorya fischeri p1, and successfully expressed in pichia pastoris. the predicted amino acid sequence of man5p1 consists of a putative 19-residue signal peptide at the n-terminus and a catalytic domain of glycoside hydrolase family 5. the purified recombinant man5p1 (rman5p1) was optimally active at ph 4.0 and 80 °c, and was acid and alkali tolerant, exhibiting >20% of the maximal activity at ph 2.0 and 9.0. rman5p1 had ... | 2015 | 25466024 |
| modeling the growth of byssochlamys fulva and neosartorya fischeri on solidified apple juice by measuring colony diameter and ergosterol content. | byssochlamys fulva and neosartorya fischeri are heat-resistant fungi which are a concern to food industries (e.g. apple juice industry) since their growth represents significant economic liabilities. although the most common method used to assess fungal growth in solid substrates is by measuring the colony's diameter, it is difficult to apply this method to food substrates. alternatively, ergosterol contents have been used to quantify fungal contamination in some types of food. the current study ... | 2015 | 25462919 |
| loop 3 of fungal endoglucanases of glycoside hydrolase family 12 modulates catalytic efficiency. | glycoside hydrolase (gh) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. however, the important roles of the loop regions of gh12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. this study examined how the loop 3 region affects the enzymatic properties of gh12 glucanases using nfeg12a from neosartorya fischeri p1 and eg (pdb 1ks4) from aspergillus niger acidophilic and thermophilic nfeg12a had the hig ... | 2017 | 28039140 |
| occurrence of azole-resistant species of aspergillus in the uk environment. | the aim of this study was to survey environmental isolates of aspergillus resistant to azoles in azole-treated and naïve areas to determine whether resistance could be related to azole treatment history. aspergillus fumigatus was sampled from the centre of a large city and from fields with known azole history. azole resistance was determined and sequencing was performed to identify strains and mutations in the cyp51a gene. azole resistance was detected in azole-treated field isolates but not in ... | 2014 | 27873687 |
| the human ku autoantigen shares amino acid sequence homology with fungal, but not bacterial and viral, proteins. | context: molecular mimicry between autoantigens and microbial antigens is a possible triggering mechanism of autoimmunity. human ku is a dna-associated autoantigen targeted by autoantibodies in patients with systemic lupus erythematosus and related disorders; available data are consistent with a role of molecular mimicry in the pathogenesis of anti-ku autoimmunity, but no research exist on this topic. objective: we aimed to define the most probable microbial triggers of anti-ku autoimmunity via ... | 2011 | 20718578 |
| a penicillium chrysogenum-based expression system for the production of small, cysteine-rich antifungal proteins for structural and functional analyses. | small, cysteine-rich and cationic antifungal proteins (aps) from filamentous ascomycetes, such as nfap from neosartorya fischeri and paf from penicillium chrysogenum, are promising candidates for novel drug development. a prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. technologies for structure analyses, such as electronic circular dichroism (ecd) ... | 2016 | 27835989 |
| survey of molds, yeast and alicyclobacillus spp. from a concentrated apple juice productive process. | bacteria and molds may spoil and/or contaminate apple juice either by direct microbial action or indirectly by the uptake of metabolites as off-flavours and toxins. some of these microorganisms and/or metabolites may remain in the food even after extensive procedures. this study aim to identify the presence of molds (including heat resistant species) and alicyclobacillus spp., during concentrated apple juice processing. molds were isolated at different steps and then identified by their macrosco ... | 2014 | 24948913 |
| a promiscuous prenyltransferase from aspergillus oryzae catalyses c-prenylations of hydroxynaphthalenes in the presence of different prenyl donors. | prenyltransferases of the dimethylallyltryptophan synthase (dmats) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. most members of this superfamily accepted as prenyl donor exclusively dimethylallyl diphosphate (dmapp). one enzyme, anapt from neosartorya fischeri, was reported recently to use both dmapp and geranyl diphosphate (gpp) as prenyl do ... | 2014 | 24430210 |
| a thermophilic α-galactosidase from neosartorya fischeri p1 with high specific activity, broad substrate specificity and significant hydrolysis ability of soymilk. | an extracellular α-galactosidase (gal27a) with high specific activity of 423umg(-1) was identified in thermophilic neosartorya fischeri p1. its coding gene (1680bp) was cloned and functionally expressed in pichia pastoris. sequence analysis indicated that deduced gal27a contains a catalytic domain of glycoside hydrolase family 27. the native and recombinant enzymes shared some similar properties, such as ph optima at 4.5, temperature optima at 60-70°c, resistance to most chemicals and saccharide ... | 2014 | 24360500 |
| genome mining for new α-amylase and glucoamylase encoding sequences and high level expression of a glucoamylase from talaromyces stipitatus for potential raw starch hydrolysis. | mining fungal genomes for glucoamylase and α-amylase encoding sequences led to the selection of 23 candidates, two of which (designated tsgam-2 and nfamy-2) were advanced to testing for cooked or raw starch hydrolysis. tsgam-2 is a 66-kda glucoamylase recombinantly produced in pichia pastoris and originally derived for talaromyces stipitatus. when harvested in a 20-l bioreactor at high cell density (od600 > 200), the secreted tsgam-2 enzyme activity from p. pastoris strain gs115 reached 800 u/ml ... | 2014 | 24046254 |
| geranylation of cyclic dipeptides by the dimethylallyl transferase anapt resulting in a shift of prenylation position on the indole ring. | the dimethylallyl transferase anapt from neosartorya fischeri is involved in the biosynthesis of acetylaszonalenin and catalyses the regioselective and stereospecific c3α-prenylation of (r)-benzodiazepinedione in the presence of dimethylallyl diphosphate. this enzyme also converts several tryptophan-containing cyclic dipeptides to c3α-prenylated indolines. in this study, we demonstrate the geranylation of (r)-benzodiazepinedione and five other cyclic dipeptides by anapt in the presence of gerany ... | 2013 | 24014429 |
| clinical significance and molecular characterization of nonsporulating molds isolated from the respiratory tracts of bronchopulmonary mycosis patients with special reference to basidiomycetes. | nonsporulating molds (nsms), especially basidiomycetes, have predominantly been reported as human pathogens responsible for allergic and invasive disease. their conventional identification is problematic, as many isolates remain sterile in culture. thus, inconclusive culture reports might adversely affect treatment decisions. the clinical significance of nsms in pulmonary mycoses is poorly understood. we sequenced the internal transcribed spacer (its) region and d1/d2 domain of the larger subuni ... | 2013 | 23903552 |
| functional properties of a manganese-activated exo-polygalacturonase produced by a thermotolerant fungus aspergillus niveus. | a thermotolerant fungus identified as aspergillus niveus was isolated from decomposing materials and it has produced excellent levels of hydrolytic enzymes that degrade plant cell walls. a. niveus germinated faster at 40 °c, presenting protein levels almost twofold higher than at 25 °c. the crude extract of the a. niveus culture was purified by diethylaminoethyl (deae)-cellulose, followed by biogel p-100 column. polygalacturonase (pg) is a glycoprotein with 37.7 % carbohydrate, molecular mass of ... | 2013 | 23613286 |
| effect of different fruit components, aeration and cold storage at 5°c on the germination of ascospores of neosartorya fischeri. | germination is the process by which a spore is transformed from a dormant state of metabolic activity to one of high activity. effects of different fruit components, aeration and cold storage at 5°c on the germination of ascospores of neosartorya fischeri after heat treatment were investigated by measuring rate and percentage germination and outgrowth of fungal biomass. some activated spores reverted back to dormancy after 12 and 24 h of storage at 5°c. ascospores germinated easily in acetate bu ... | 2013 | 23530777 |
| genome mining of a prenylated and immunosuppressive polyketide from pathogenic fungi. | activation of the polycyclic polyketide prenyltransferase (pcptase)-containing silent clusters in aspergillus fumigatus and neosartorya fischeri led to isolation of a new metabolite neosartoricin (3). the structure of 3 was solved by x-ray crystallography and nmr to be a prenylated anthracenone. 3 exhibits t-cell antiproliferative activity with an ic(50) of 3 μm, suggestive of a physiological role as an immunosuppressive agent. | 2013 | 23368997 |
| complexity generation in fungal peptidyl alkaloid biosynthesis: a two-enzyme pathway to the hexacyclic mdr export pump inhibitor ardeemin. | ardeemins are hexacyclic peptidyl alkaloids isolated from aspergillus fischeri as agents that block efflux of anticancer drugs by multidrug resistance (mdr) export pumps. to evaluate the biosynthetic logic and enzymatic machinery for ardeemin framework assembly, we sequenced the a. fischeri genome and identified the ardabc gene cluster. through both genetic deletions and biochemical characterizations of purified arda and ardb we show this ardab enzyme pair is sufficient to convert anthranilate ( ... | 2013 | 23330675 |
| sequencing of mitochondrial genomes of nine aspergillus and penicillium species identifies mobile introns and accessory genes as main sources of genome size variability. | the genera aspergillus and penicillium include some of the most beneficial as well as the most harmful fungal species such as the penicillin-producer penicillium chrysogenum and the human pathogen aspergillus fumigatus, respectively. their mitochondrial genomic sequences may hold vital clues into the mechanisms of their evolution, population genetics, and biology, yet only a handful of these genomes have been fully sequenced and annotated. | 2012 | 23234273 |
| investigation of the antimicrobial effect of neosartorya fischeri antifungal protein (nfap) after heterologous expression in aspergillus nidulans. | neosartorya fischeri antifungal protein (nfap) is a β-defensin-like peptide produced by the n. fischeri nrrl 181 isolate. in this study, we investigated the manifestation of the antimicrobial effect of nfap via heterologous expression of the nfap gene in an nfap-sensitive fungus, aspergillus nidulans. heterologous expression of the nfap gene was carried out in a. nidulans cs2902 using a pama1-based autonomous replicative vector construct. the effect of the produced nfap on the germination of a. ... | 2013 | 23197172 |
| identification of the verruculogen prenyltransferase ftmpt3 by a combination of chemical, bioinformatic and biochemical approaches. | previous studies showed that verruculogen is the end product of a biosynthetic gene cluster for fumitremorgin-type alkaloids in aspergillus fumigatus and neosartorya fischeri. in this study, we isolated fumitremorgin a from n. fischeri. this led to the identification of the responsible gene, ftmpt3, for o-prenylation of an aliphatic hydroxy group in verruculogen. this gene was found at a different location in the genome of n. fischeri than the identified cluster. the coding sequence of ftmpt3 wa ... | 2012 | 23109474 |
| detection and purification of non-ribosomal peptide synthetase products in neosartorya fischeri. | non-ribosomal peptide synthetases (nrpss) are important enzymes of production machinery for natural products including clinically used antibiotics, antifungal, and anticancer agents. nrps products are usually further modified by tailoring enzymes, resulting in the formation of diverse structures. we demonstrate here the production and isolation of metabolites produced by two bi-modular nrpss, i.e., acetylaszonalenin and fumitremorgin-type alkaloids in neosartorya fischeri nrrl181. | 2012 | 23065611 |
| production of a defensin-like antifungal protein nfap from neosartorya fischeri in pichia pastoris and its antifungal activity against filamentous fungal isolates from human infections. | neosartorya fischeri nrrl 181 isolate secretes a defensin-like antifungal protein (nfap) which has a remarkable antifungal effect against ascomycetous filamentous fungi. this protein is a promising antifungal agent of biotechnological value; however in spite of the available knowledge of the nature of its 5'-upstream transcriptional regulation elements, the bulk production of nfap has not been resolved yet. in this study we carried out its heterologous expression in the yeast pichia pastoris and ... | 2014 | 24269762 |
| cdpc2pt, a reverse prenyltransferase from neosartorya fischeri with a distinct substrate preference from known c2-prenyltransferases. | a putative prenyltransferase gene, nfia_043650, was amplified from neosartorya fischeri nrrl 181 and cloned into the expression vector pqe60. the deduced polypeptide consisting of 445 amino acids with a molecular mass of 51 kda was overproduced in escherichia coli and purified as his6-tagged protein to near homogeneity. the purified soluble protein was subsequently assayed with potential aromatic substrates in the presence of dimethylallyl diphosphate. hplc analysis of the reaction mixtures reve ... | 2013 | 23845975 |
| cloning and characterization of f3pyc gene encoding pyruvate carboxylase in aspergillus flavus strain (f3). | pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and l-malic acid. these organic acids play very important role for biological remediation of heavy metals. in this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (f3pyc) from heavy metal resistant indigenous fungal isolate aspergillus flavus (f3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a c ... | 2017 | 28710744 |
| aspergillus fischeri mediated biosynthesis of gold nanoparticles and their beneficially comparative effect on normal and cancer cell lines. | background along with the intensified use of metal nanoparticles, growing concern of their adverse outcome on human health has also expanded, indicating that this work is an integral part of nanobioscience study. objective the aim of this study was to evaluate varied effect of biosynthesized gold nanoparticles (aunps) on normal and cancerous mammalian cells. methods aunp synthesized and characterized by different characterization methods, here are produced by specifically isolated aspergillus sp ... | 2017 | 28530537 |
| structural determinants of neosartorya fischeri antifungal protein (nfap) for folding, stability and antifungal activity. | the recent global challenges to prevent and treat fungal infections strongly demand for the development of new antifungal strategies. the structurally very similar cysteine-rich antifungal proteins from ascomycetes provide a feasible basis for designing new antifungal molecules. the main structural elements responsible for folding, stability and antifungal activity are not fully understood, although this is an essential prerequisite for rational protein design. in this study, we used the neosart ... | 2017 | 28512317 |
| molecular characterization of a highly-active thermophilic β-glucosidase from neosartorya fischeri p1 and its application in the hydrolysis of soybean isoflavone glycosides. | isoflavone occurs abundantly in leguminous seeds in the form of glycoside and aglycone. however, isoflavone glycoside has anti-nutritional effect and only the free type is beneficial to human health. in the present study we identified a β-glucosidase from thermophilic neosartorya fischeri p1, termed nfbgl1, capable of efficiently converting isoflavone glycosides into free isoflavones. the gene, belonging to glycoside hydrolase family 3, was successfully overexpressed in pichia pastoris at high c ... | 2014 | 25188254 |
| a decrease in bulk water and mannitol and accumulation of trehalose and trehalose-based oligosaccharides define a two-stage maturation process towards extreme stress resistance in ascospores of neosartorya fischeri (aspergillus fischeri). | fungal propagules survive stresses better than vegetative cells. neosartorya fischeri, an aspergillus teleomorph, forms ascospores that survive high temperatures or drying followed by heat. not much is known about maturation and development of extreme stress resistance in fungal cells. this study provides a novel two-step model for the acquisition of extreme stress resistance and entry into dormancy. ascospores of 11- and 15-day-old cultures exhibited heat resistance, physiological activity, acc ... | 2015 | 25040022 |