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analysis of xyloglucan endotransglycosylase/hydrolase (xth) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening.xyloglucan endotransglycosylase/hydrolase (xth) enzymes have played a role in the remodeling of cell wall hemicelluloses. to investigate the function of xths in persimmon (diospyros kaki l.) fruit development and postharvest softening, five cdnas (dkxth1 to dkxth5), whose putative proteins contained the conserved deidfeflg motif of xth, were cloned. real time quantitative pcr analysis revealed that dkxth1, dkxth4, and dkxth5 peaked in immature expanding fruit, and their higher expression was obs ...201525849978
persimmon (diospyros kaki l.) leaves: a review on traditional uses, phytochemistry and pharmacological properties.persimmon (diospyros kaki l.) leaves, known as shi ye (in chinese), have a long history as a chinese traditional medicine for the treatment of ischemia stroke, angina, internal hemorrhage, hypertension, atherosclerosis and some infectious diseases, etc. additionally, persimmon leaves could be used as healthy products, cosmetics and so on, which have become increasingly popular in asia, such as japan, korea and china etc.201525637828
physicochemical characterization of pure persimmon juice: nutritional quality and food acceptability.technological process for production of non-astringent persimmon (diospyros kaki thunb. cv. "rojo brillante") juice was described. the degree of fruit ripening expressed as color index (ci) varied between 12.37 and 16.33. persimmon juice was characterized by determining physicochemical quality parameters as yield, total soluble solids (tss), ph, titratable acidity (ta), organic acids, and main sugars. a thermal treatment of 90 ºc for 10 s was effective in controlling naturally occurring microorg ...201525619747
identification and characterization of micrornas from chinese pollination constant non-astringent persimmon using high-throughput sequencing.micrornas (mirnas) have been shown to play key roles in regulating gene expression at post-transcriptional level, but mirnas associated with natural deastringency of chinese pollination-constant nonastringent persimmon (cpcna) have never been identified.201525604351
persimmon (diospyros kaki) fruit: hidden phytochemicals and health claims.currently, nutrition and health linkages focused on emerging strategy of diet based regimen to combat various physiological threats including cardiovascular disorders, oxidative stress, diabetes mellitus, etc. in this context, consumption of fruits and vegetables is gaining considerable importance as safeguard to maintain human health. likewise, their phytochemicals and bioactive molecules are also becoming popular as promising demulcent against various ailments. the current review is an effort ...201527047315
mineral profile of kaki fruits (diospyros kaki l.).the main objective of this study was the determination of the mineral profile of 167 kaki fruit (diospyros kaki l.) samples produced from different regions of spain, including samples with the protected designation of origin (pdo) 'kaki ribera del xúquer' valencia (spain). samples were analysed by inductively coupled plasma optical emission spectroscopy (icp-oes) and inductively coupled plasma mass spectrometry (icp-ms). consumption of one piece of kaki fruit (200-400 g) would give a mineral int ...201525442556
discovery and molecular characterization of a new cryptovirus dsrna genome from japanese persimmon through conventional cloning and high-throughput sequencing.through the application of next generation sequencing, in synergy with conventional cloning of dop-pcr fragments, two double-stranded rna (dsrna) molecules of about 1.5 kbp in size were isolated from leaf tissue of a japanese persimmon (accession sspi) from apulia (southern italy) showing veinlets necrosis. high-throughput sequencing allowed whole genome sequence assembly, yielding a 1,577 and a 1,491 bp contigs identified as dsrna-1 and dsrna-2 of a previously undescribed virus, provisionally n ...201525315633
rapid screening of low-molecular-weight phenols from persimmon (diospyros kaki) pulp using liquid chromatography/uv-visible/electrospray mass spectrometry analysis.persimmon fruits have been widely used in traditional medicine owing to their phenolic composition. this research aims to perform a rapid, detailed and affordable study of the profile of low-molecular-weight phenols from persimmon pulp.201525103690
effect of deastringency treatment of intact persimmon fruits on the quality of fresh-cut persimmons.the changes in the quality characteristics of the fresh-cut products prepared from intact 'cheongdobansi' persimmons treated with different deastringency methods (nontreated, carbon dioxide gas, warm water, ethanol vapour) have been investigated for 6 days at 10 °c. flesh firmness of the persimmons decreased after ethanol vapour treatment. the decrease in l(∗) value and flesh firmness in the slices prepared from persimmons treated with warm water was retarded. soluble solids content and titratab ...201525053045
studies of the microbial metabolism of flavonoids extracted from the leaves of diospyros kaki by intestinal bacteria.flavonoid glycosides are metabolized by intestinal bacteria, giving rise to a wide range of phenolic acids that may exert systemic effects in the body. the microbial metabolism of flavonoids extracted from the leaves of diospyros kaki (fldk) by intestinal bacteria was investigated in vitro. high-performance liquid chromatography/linear trap quadrupole orbitrap mass spectrometry was performed to analyze the metabolites of flavonoids in vivo using xcalibur2.1 software. the results showed that the ...201525011569
trait-mediated seed predation, dispersal and survival among frugivore-dispersed plants in a fragmented subtropical forest, southwest china.by tracking the fate of individual seeds from 6 frugivore-dispersed plants with contrasting seed traits in a fragmented subtropical forest in southwest china, we explored how rodent seed predation and hoarding were influenced by seed traits such as seed size, seed coat hardness and seed profitability. post-dispersal seed fates varied significantly among the 6 seed species and 3 patterns were witnessed: large-seeded species with a hard seed coat (i.e. choerospoadias axillaries and diospyros kaki ...201424952965
short communication: development and characterization of novel transcriptome-derived microsatellites for genetic analysis of persimmon.oriental persimmon (diospyros kaki thunb.) (2n = 6x = 90) is a major commercial and deciduous fruit tree that is believed to have originated in china. however, rare transcriptomic and genomic information on persimmon is available. using roche 454 sequencing technology, the transcriptome from rna of the flowers of d. kaki was analyzed. a total of 1,250,893 reads were generated and 83,898 unigenes were assembled. a total of 42,711 ssr loci were identified from 23,494 unigenes and 289 polymerase ch ...201424782136
partial purification and characterization of polyphenol oxidase from persimmon.activity of polyphenol oxidase (ppo) from "rojo brillante" persimmon (diospyros kaki l.) fruits was characterized. crude extracts were used for characterization of enzyme activity and stability at different temperatures (60, 70 and 80 °c), phs (from 3.5 to 7.5) and substrate concentrations (catechol from 0 to 0.5m). maximum enzyme activity was reached at ph 5.5 and 55 °c. enzyme stability was higher than ppo activities found in other natural sources, since above ph 5.5 the minimum time needed to ...201424679782
genome-wide transcriptome analysis of chinese pollination-constant nonastringent persimmon fruit treated with ethanol.the persimmon diospyros kaki thunb. is an important commercial and deciduous fruit tree. the fruits have proanthocyanidin (pa) content of >25% of the dry weight and are astringent. pas cause astringency that is often undesirable for human consumption; thus, the removal of astringency is an important practice in the persimmon industry. soluble pas can be converted to insoluble pas by enclosing the fruit in a polyethylene bag containing diluted ethanol. the genomic resource development of the pers ...201424507483
flavonoids from persimmon (diospyros kaki) leaves (fpl) attenuate h2o2-induced apoptosis in mc3t3-e1 cells via the nf-κb pathway.the leaves of persimmon (diospyros kaki l.) have long been used in chinese medicine for the treatment of paralysis, frostbite, burns, and to stop bleeding. flavonoids of persimmon leaves (fpl) are known for their antioxidant activity in murine osteoblast mc3t3-e1 cells, but their mechanisms in osteoblast cells injured by oxidative stress are unknown. in this study, the effects of fpl on oxidative damage were investigated by addressing their potential therapeutic or toxic effects on h2o2-stimulat ...201424488014
persimmon peel extract attenuates pdgf-bb-induced human aortic smooth muscle cell migration and invasion through inhibition of c-src activity.the unregulated migration and invasion of human aortic smooth muscle cells (hasmcs) into the intima is a crucial step in the development of atherosclerosis. recently, the oriental persimmon extract (diospyros kaki thunb. cv. fuyu) has been investigated for its anti-atherogenic properties, but the molecular mechanisms involved remain unclear. we investigated the inhibitory effects of persimmon peel and flesh extract on the platelet-derived growth factor (pdgf) bb-induced mmp-1 expression using we ...201323993486
inhibitory effects of diospyros kaki in a model of allergic inflammation: role of camp, calcium and nuclear factor-κb.diospyros kaki (d. kaki) has been cultivated throughout eastern asia for hundreds of years. d. kaki contains various biological active compounds, such as amino acids, carotenoids, flavonoids, tannins, catechins and vitamin a. previous studies have shown that d. kaki has beneficial effects on homeostasis, constipation, hypertension, atherosclerosis and allergic dermatitis and is a good source of antioxidants, polyphenols and dietary fiber. however, the anti-allergic and anti-inflammatory effects of ...201323921373
investigation of diospyros kaki l.f husk extracts as corrosion inhibitors and bactericide in oil field.hydrochloric acid is used in oil-well acidizing commonly for improving the crude oil production of the low-permeable reservoirs, while it is a great challenge for the metal instruments involved in the acidification. developing natural products as oilfield chemicals is a straight way to find less expensive, green and eco-friendly materials. the great plant resources in qin-ling and ba-shan mountain area of shannxi province enable the investigating of new green oil field chemicals. diospyros kaki ...201323816431
characterization of carotenoid fatty acid esters from the peels of the persimmon diospyros kaki.separation and structural determination of the chloroform-soluble components obtained from the peels of the persimmon (diospyros kaki thunb.) were performed. β-carotene, lycopene, β-cryptoxanthin mono-myristic acid ester, zeaxanthin di-myristic acid ester, the latter two of which were accompanied by a small amount of palmitoleic acid in the fatty acid moiety, and oleanolic acid were identified. among these components, the mono-fatty acid ester of β-cryptoxanthin and the di-fatty acid ester of ze ...201323727781
characterization and comparison of est-ssr and trap markers for genetic analysis of the japanese persimmon diospyros kaki.we developed and characterized expressed sequence tags (ests)-simple sequence repeats (ssrs) and targeted region amplified polymorphism (trap) markers to examine genetic relationships in the persimmon genus diospyros gene pool. in total, we characterized 14 est-ssr primer pairs and 36 trap primer combinations, which were amplified across 20 germplasms of 4 species in the genus diospyros. we used various genetic parameters, including effective multiplex ratio (emr), diversity index (di), and mark ...201323359055
diospyrobezoar as a cause of small bowel obstruction.phytobezoar, a concretion of indigestible fibers derived from ingested vegetables and fruits, is the most common type of bezoar. diospyrobezoar is a subtype of phytobezoar formed after excessive intake of persimmons (diospyros kaki). we report the case of a diabetic man with a 5-day history of abdominal pain after massive ingestion of persimmons who developed signs of complicated small bowel obstruction. the patient had a previous history of billroth ii hemigastrectomy associated with truncal va ...201223271989
isolation and characterization of a laccase gene potentially involved in proanthocyanidin polymerization in oriental persimmon (diospyros kaki thunb.) fruit.proanthocyanidins (pas, condensed tannins) are important health-promoting phytochemicals that are abundant in many plants. oriental persimmon (diospyros kaki thunb.) is an excellent source of pas because of its unique ability to accumulate large quantities of these compounds in its young fruit. there are two different spontaneous mutant phenotypes of oriental persimmons which lose their astringent taste naturally on the tree; while plants without these mutations remain rich in soluble pas until ...201323224657
in vitro propagation of persimmon (diospyros kaki thunb.).persimmon (diospyros kaki thunb.) is a temperate fruit tree species diffused in all continents. the traditional propagation method adopted by the nursery industry is based on budding/grafting scion cultivars on seedlings from d. kaki, diospyros lotus, and diospyros virginiana, the most important species used as rootstock, reproduced by seeds since they are not easy to root. furthermore, most of nonastringent cultivars of persimmon are not compatible with d. lotus, a rootstock largely utilized be ...201323179692
persimmon fruit tannin-rich fiber reduces cholesterol levels in humans.bile acid-binding agents are known to lower blood cholesterol levels and have been clinically used for the treatment of hypercholesterolemia. we previously showed that tannin-rich fiber from young persimmon (diospyros kaki) fruits had bile acid-binding properties. in this study, we performed a randomized, double-blind, placebo-controlled trial to investigate the hypocholesterolemic effects of tannin-rich fiber in humans. the subjects (n = 40, plasma total cholesterol levels 180-259 mg/dl) were d ...201323171573
occurrence of sooty blotch and flyspeck disease on sweet persimmon in korea.sooty blotch and flyspeck (sbfs), a disease caused by a complex of fungi, results in substantial economic losses for commercial growers of sweet persimmon (diospyros kaki l.) in korea. however, many species causing sbfs in korea have not been identified and sources of inoculum are uncertain. based on mycological characteristics, pathogenicity, and molecular data, the causal fungi were identified as dissoconium sp. and zygophiala wisconsinensis. this is the first report of sbfs of sweet persimmon ...201223115517
ethylene-responsive transcription factors interact with promoters of adh and pdc involved in persimmon (diospyros kaki) fruit de-astringency.the persimmon fruit is a particularly good model for studying fruit response to hypoxia, in particular, the hypoxia-response erf (hre) genes. an anaerobic environment reduces fruit astringency by converting soluble condensed tannins (scts) into an insoluble form. although the physiology of de-astringency has been widely studied, its molecular control is poorly understood. both co(2) and ethylene treatments efficiently removed the astringency from 'mopan' persimmon fruit, as indicated by a decrea ...201223095993
sulfation modification and anticoagulant activity of the polysaccharides obtained from persimmon (diospyros kaki l.) fruits.the optimal conditions for sulfation of polysaccharides from persimmon fruits (pfp) with chlorosulfonic acid-pyridine (csa-pyr) method were determined by response surface methodology. box-behnken design was applied to evaluate the effects of three independent variables (volume ratio of pyr to csa, volume ratio of pfp to so(3)pyr and reaction time) on the degree of substitution (ds), molecular weight (mw) and activated partial thromboplastin time (aptt) of sulfated polysaccharides (pfp-s). the ap ...201222960188
a comparative study on the in vitro antioxidant potentials of three edible fruits: cornelian cherry, japanese persimmon and cherry laurel.this study was designed in order to investigate in vitro antioxidant potentials of 80% methanolic extracts prepared from three edible fruits, cornus mas l., diospyros kaki l., laurocerasus officinalis roem. for this purpose, 8 different tests were performed including 2,2-diphenyl-1-picrylhydrazyl (dpph) and superoxide radical scavenging tests, ferric reducing antioxidant power (frap), cupric reducing antioxidant capacity (cuprac), metal-chelating capacity, determination of total antioxidant capa ...201222713712
comparative studies of antioxidant activities and nutritional constituents of persimmon juice (diospyros kaki l. cv. gapjubaekmok).the objectives of this research were to evaluate antioxidant activities and nutritional components, including phenolic acid, catechin, organic acid, sugar, and amino acid, of persimmon juice from persimmons grown in different regions around korea. persimmon (diospyros kaki) exhibits potent antioxidant effects in dpph, abts, reducing power, and frap methods of analysis. the levels of nutritional constituents showed significant differences among all the samples. in particular, tartaric acid, gluco ...201224471076
bioinformatic analysis of fruit-specific expressed sequence tag libraries of diospyros kaki thunb.: view at the transcriptome at different developmental stages.we present here a systematic analysis of the diospyros kaki expressed sequence tags (ests) generated from development stage-specific libraries. a total of 2,529 putative tentative unigenes were identified in the mf library whereas the oyf library displayed 3,775 tentative unigenes. among the two cdna libraries, 325 est-simple sequence repeats (ssrs) in 296 putative unigenes were detected in the mf library showing an occurrence of 11.7% with a frequency of 1 ssr/3.16 kb whereas the oyf library ha ...201122558534
cryopreservation of in vitro grown shoot tips of diospyros kaki thunb. using different methods.the objective of this study was to compare the potential of different cryopreservation strategies for in vitro shoot tips of diospyros kaki thunb. the treatments consisted of three different cryopreservation methods: vitrification, droplet-vitrification and modified droplet-vitrification. the following variables were assessed: cold acclimation, sucrose concentration in the preculture medium and pvs2 treatment time. a higher average survival level was obtained using the modified droplet-vitrifica ...201322434124
water-insoluble condensed tannins content of young persimmon fruits-derived crude fibre relates to its bile acid-binding ability.in this study, we prepared crude fibre samples from young fruits of 10 persimmon (diospyros kaki) cultivars and from young fruits of japanese pear (pyrus pyrifolia var. culta) and peach (prunus persica) and compared their bile acid-binding abilities in vitro. all crude fibre samples from young fruits of persimmon were able to bind to cholic acid in a 4 mm cholic acid solution, but crude fibre samples from young fruits of japanese pear and peach did not show bile acid-binding ability. the bile ac ...201222250751
seasonal abscisic acid signal and a basic leucine zipper transcription factor, dkbzip5, regulate proanthocyanidin biosynthesis in persimmon fruit.proanthocyanidins (pas) are secondary metabolites that contribute to plant protection and crop quality. persimmon (diospyros kaki) has a unique characteristic of accumulating large amounts of pas, particularly in its fruit. normal astringent-type and mutant nonastringent-type fruits show different pa accumulation patterns depending on the seasonal expression patterns of dkmyb4, which is a myb transcription factor (tf) regulating many pa pathway genes in persimmon. in this study, attempts were ma ...201222190340
sulphated modification of a polysaccharide obtained from fresh persimmon (diospyros kaki l.) fruit and antioxidant activities of the sulphated derivatives.free radicals and other reactive oxygen species (ros) are believed to play significant roles in ageing as well as in a number of degenerative or pathological diseases. this paper reports the preparation, characterisation and potential antioxidant activity of a type of chemically sulphated polysaccharide isolated from fresh persimmon (diospyros kaki l.) fruit. three sulphated derivatives with variable degrees of substitution (0.8, 1.7 and 2.5) were obtained by the chlorosulphonic acid-pyridine me ...201125214099
genetic relationships of the japanese persimmon diospyros kaki (ebenaceae) and related species revealed by ssr analysis.simple sequence repeat (ssr) molecular markers based on 18 primers were employed to study the genetic relationship of japanese persimmon (diospyros kaki) specimens. two hundred and sixty-two bands were detected in 30 japanese persimmon samples, including 14 japanese and 10 chinese genotypes of japanese persimmon (diospyros kaki) and six related species, d. lotus, d. glaucifolia, d. oleifera, d. rhombifolia, d. virginiana, and jinzaoshi (unclassified - previously indicated to be d. kaki). all ssr ...201121710456
microbial activity and organic matter dynamics during 4 years of irrigation with treated wastewater.the global changes in rainfall frequency and quantity have subjected arid and semi-arid regions to long periods of drought. as this phenomenon corresponds to increasing trend of water shortage, the use of treated wastewater (tww) has been suggested as an alternative for irrigation of agricultural crops in these areas. the aim of the study was to investigate the short- and middle-term effects of tww irrigation on the soil microbial activities and organic carbon content. the microbial community ac ...201121584754
molecular cloning and expression of squalene synthase and 2,3-oxidosqualene cyclase genes in persimmon (diospyros kaki l.) fruits.oleanolic acid (oa) and ursolic acid (ua) are the main triterpene acids in persimmon fruit, and squalene synthase and 2,3-oxidosqualene cyclases are important enzymes in pentacyclic triterpene biosynthesis. in order to study their relationship, dksqs and dkosc were cloned from persimmon fruits in the present study. the full-length cdna of dksqs was 1647 bp, containing an open reading frame (orf) of 1245 bp that encoded a peptide of 415 amino acids (aa). the 3'-end of dkosc cdna fragment containe ...201221573791
isolation and tyrosinase inhibitory effects of polyphenols from the leaves of persimmon, diospyros kaki.the main polyphenols were isolated from the leaves of six selected persimmon cultivars. seven compounds were obtained by reverse-phase hplc, and their structures were elucidated by multiple nmr measurements. these compounds are hyperoside, isoquercitrin, trifolin, astragalin, chrysontemin, quercetin-3-o-(2''-o-galloyl-β-d-glucopyranoside) (qog), and kaempferol-3-o-(2''-o-galloyl-β-d-glucopyranoside) (kog). their inhibitory activity was tested against tyrosinase for the oxidation of l-dopa, and o ...201121568325
vomifoliol 9-o-α-arabinofuranosyl (1→6)-β-d-glucopyranoside from the leaves of diospyros kaki stimulates the glucose uptake in hepg2 and 3t3-l1 cells.a novel α-glucosidase inhibitor, vomifoliol 9-o-α-arabinofuranosyl (1→6)-β-d-glucopyranoside, was isolated for the first time from leaves of diospyros kaki and its bioactivity analyzed. this inhibitor exhibited strong anti-α-glucosidase activity with an ic50 value of 170.62nm and stimulated a dose-dependent increase in the uptake of a fluorescent d-glucose analog, 2-[n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-nbdg), in hepg2 cells at a rate higher than that of insulin contr ...201121555120
effects of seasonal temperature changes on dkmyb4 expression involved in proanthocyanidin regulation in two genotypes of persimmon (diospyros kaki thunb.) fruit.persimmon fruits accumulate a large amount of proanthocyanidin (pa). fruits of the mutant non-astringent (na) type lose their ability to accumulate pa at an early stage of fruit development, whereas fruits of the normal astringent (a) type sustain pa accumulation until ripening. this allelotype is determined by the genotype of a single astringency (ast) locus. it is possible that the reduction in pa accumulation in na-type fruits is due to phenological down-regulation of dkmyb4 (a pa regulator) ...201121225280
cloning of phytoene desaturase and expression analysis of carotenogenic genes in persimmon (diospyros kaki l.) fruits.persimmon is a commercially important fruit crop, and the fruit is rich in different kinds of bioactive compounds, among which carotenoids contribute significantly to its color and nutritional value. in this study, the cdna of phytoene desaturase gene (pds) was isolated by rapid amplification of cdna ends (race) technique. sequence analysis indicated that the full-length cdna of pds was 2064 bp, encoding 586 amino acids and containing one open reading frame (orf) of 1761 bp. homology analysis sh ...201121116859
variation of oleanolic and ursolic acid in the flesh of persimmon fruit among different cultivars.oleanolic acid (oa) and ursolic acid (ua) are important bioactive components in many plants, including persimmon (diospyros kaki l.). the present work was carried out to determine oa and ua contents in the flesh of persimmon fruit from 32 cultivars, including 23 astringent and 9 non-astringent ones, by applying high performance liquid chromatography (hplc) with uv detection. both oa and ua were present in all of the investigated cultivars, except for three, 'hiratanenashi', 'ribenhongshi' and 'm ...201020877245
high molecular weight persimmon (diospyros kaki l.) proanthocyanidin: a highly galloylated, a-linked tannin with an unusual flavonol terminal unit, myricetin.maldi-tof ms suggested that the high molecular weight proanthocyanidin (condensed tannin) from persimmon (diospyros kaki l.) pulp comprised a heteropolyflavanol series with flavan-3-o-galloylated extenders, flavan-3-ol and flavonol terminal units, and a-type interflavan linkages. thiolysis-hplc-esi-ms with dad, electrochemical, and esi-ms detection confirmed a previously unreported terminal unit, the flavonol myricetin, in addition to the typical flavan-3-ols catechin and epigallocatechin gallat ...201023654234
chemical structure and immunoinhibitory activity of a pectic polysaccharide containing glucuronic acid from the leaves of diospyros kaki.pectin is a family of complex polysaccharides characterized by the presence of 1,4-linked alpha-galacturonic acid residues in the backbone and is more recognized for its potential bioactive properties. herein we reported the structural and biological features of a pectic fraction dl-4oac-1 from the leaves of diospyros kaki. chemical and spectral analysis revealed this polysaccharide possessed a backbone composed of a repeating disaccharide [-->4)-alpha-galap-(1-->2)-alpha-rhap-(1-->], substitute ...201020361992
characterization of a pectic polysaccharide from the leaves of diospyros kaki and its modulating activity on lymphocyte proliferation.pectin is a group of carbohydrate polymers constructing the primary cell walls and the middle lamella of terrestrial plants. herein, we demonstrated the structure and immunomodulatory activity of the major pectic polysaccharide dl-3b(2) isolated from the leaves of diospyros kaki. based on composition analysis, methylation analysis, two-step acid hydrolysis, lithium-mediated selective degradation, (13)c nmr spectroscopy, and electrospray ionization mass spectrometry, dl-3b(2) was found to contain ...201020235231
a novel c-glycosylflavone from the leaves of diospyros kaki.a novel c-glycosylflavone with unusual alpha-orientation at the anomeric center of d-glucose was isolated from the leaves of diospyros kaki. its structure was determined as 8-c-[alpha-l-rhamnopyranosyl-(1 --> 4)]-alpha-d-glucopyranosylapigenin by chemical methods and spectral experiments.200920183282
[effects of climate change on photosynthesis of diospyros kaki under different soil moisture conditions].taking two-year-old diospyros kaki as test material, this paper studied the effects of high co2 concentration (700 micromol x mol(-1)), high temperature (5 degrees c higher than the mean daily temperature); and their combination on the net photosynthesis rate (pn), evapotranspiration (tr), stomatal conductance (gs), water use efficiency (wue), chlorophyll content, fv/fm, and fv/fo under different soil moisture conditions, with the ambient air temperature and co2 concentration (380 micromol x mol ...200920030133
preparing a carotenoid polyphenol-enriched extract from the peel of persimmon, diospyros kaki l.f.the use of persimmon (diospyros kaki l.f.) as a possible functional food material has not garnered widespread popularity. we conducted the present study and found that a fat-soluble extract obtained from the peel contained a large amount of carotenoids; beta-cryptoxanthin accounted for more than 50% of the total carotenoids identified. the extract also contained 6 polyphenolic aglycons.200919966462
effect of volatile emissions, especially alpha-pinene, from persimmon trees infested by japanese wax scales or treated with methyl jasmonate on recruitment of ladybeetle predators.persimmon, diospyros kaki l., is an important fruit tree in northern china. japanese wax scale, ceroplastes japonicus green (hemiptera: coccoidea: coccidae), is the most destructive pest in persimmon orchards and is difficult to control using chemical pesticides. this paper studied the variety of volatile emissions from persimmon trees attacked by wax scale or induced by applications of methyl jasmonate (meja), an exogenous signaling material. we also studied the role of volatiles in recruiting ...200919825299
expression balances of structural genes in shikimate and flavonoid biosynthesis cause a difference in proanthocyanidin accumulation in persimmon (diospyros kaki thunb.) fruit.persimmon fruits accumulate a large amount of proanthocyanidin (pa) during development. fruits of pollination-constant and non-astringent (pcna) type mutants lose their ability to produce pa at an early stage of fruit development, while fruits of the normal (non-pcna) type remain rich in pa until fully ripened. to understand the molecular mechanism for this difference, we isolated the genes involved in pa accumulation that are differentially expressed between pcna and non-pcna, and confirmed the ...200919669159
three minor novel triterpenoids from the leaves of diospyros kaki.two novel 18, 19-secoursane triterpenoids, kakisaponin b (1) and kakisaponin c (2), an ursane type 28-nortriterpene, kakidiol (3) and one known triterpenoid rosamultin (4), were isolated from the leaves of diospyros kaki. the structures of compounds 1 and 2 were determined as 28-o-beta-d-glucopyranosyl-3alpha,19,24-trihydoxy-18,19-secours-11,13(18)-dien-28-oic acid (1) and 28-o-beta-d-glucopyranosyl-2alpha,3alpha,19-trihydoxy-18,19-secours-11,13(18)-dien-28-oic acid (2) by chemical methods and s ...200919420791
persimmon cv. hachiya (diospyros kaki thunb.) fruit: some physical, chemical and nutritional properties.the persimmon cv. hachiya (diospyros kaki) fruits were analysed for some physical properties (fruit dimensions, fruit mass, fruit volume, fruit density, aspect ratio, geometric mean diameter, sphericity, spread area, projected area, bulk density, skin and flesh firmness, skin and flesh colour as l, a and b values, coefficient of static friction on different surfaces and porosity), chemical properties (moisture, ash, ph, acidity, vitamin c, total soluble solids) and nutritional properties (phosph ...200919382348
gastric outlet syndrome caused by persimmon (diospyros kaki) bezoars. 200919330741
pharmacological activity of compounds extracted from persimmon peel (diospyros kaki thunb.).persimmon peels (diospyros kaki thunb.) are discarded during the production of dried fruit. the 2-methoxy-4-vinylphenol (8) which is component of persimmon peel had high antioxidant activity on the dpph (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay and sod (superoxide dismutase) assay. and (8) had higher tyrosinase inhibiting activity than that of arbutin using both l-tyrosine and l-dopa as substrates. in addition, tyrosinase inhibiting activity of synthesized 2-methoxy-4-vinylphenol ...200919282644
triterpenoids from the leaves of diospyros kaki (persimmon) and their inhibitory effects on protein tyrosine phosphatase 1b.phytochemical study on a methanol-soluble extract of the leaves of persimmon (diospyros kaki) resulted in the isolation of two new ursane-type triterpenoids, 3alpha,19alpha-dihydroxyurs-12,20(30)-dien-24,28-dioic acid (1) and 3alpha,19alpha-dihydroxyurs-12-en-24,28-dioic acid (2), together with 12 known ursane- and oleanane-type triterpenoids (3-14). triterpenoids with a 3beta-hydroxy group were found to inhibit protein tyrosine phosphatase 1b (ptp1b) activity, with ic50 values ranging from 3.1+ ...200818798681
microsatellite isolation and characterization in japanese persimmon (diospyros kaki). 200818392932
expressed sequence tags from persimmon at different developmental stages.persimmon (diospyros kaki thunb.) is an important fruit in asian countries, where it is eaten as a fresh fruit and is also used for many other purposes. to understand the molecular mechanism of fruit development and ripening in persimmon, we generated a total of 9,952 expressed sequence tags (ests) from randomly selected clones of two different cdna libraries. one cdna library was derived from fruit of "saijo" persimmon at an early stage of development, and the other from ripening fruit. these e ...200818301901
radical scavenging activity and phenolic compounds in persimmon (diospyros kaki l. cv. mopan).the mopan persimmon (diospyros kaki l. cv. mopan) is the major cultivar of astringent persimmon in northern china. this study investigates the radical scavenging activity against abts and dpph radical, and the content of total and individual phenolics (catechin, epicatechin, epigallocatechin, chlorogenic acid, caffeic acid, and gallic acid) with apple, grape, and tomato as controls. the radical scavenging activities against abts and dpph radicals of the mopan persimmon are 23.575 and 22.597 micr ...200818211345
determination of field-incurred pyrimethanil residues in persimmon (diospyros kaki linn) by liquid chromatography.field trials have been carried out to determine the residues of the fungicide pyrimethanil in persimmon (diospyros kaki linn) after field treatment. three treatments using pyrimethanil (pyr 30% wp) were carried out on persimmon trees at a recommended dose rate: the former was sprayed at two different times at 30 and 21 days prior to harvesting; the second was sprayed three times at 40, 30 and 21 days prior to harvesting; and the third was sprayed four times at 40, 30, 21 and 14 days prior to har ...200717642069
chemical constituents of the leaves of diospyros kaki and their cytotoxic effects.isolation and structure elucidation of two new compounds, kakispyrone (1) and kakisaponin a (2), together with 11 known compounds, from the leaves of diospyros kaki l. are described. their cytotoxic effects against several cancer cell lines (a549, hepg2 and ht29) are also reported.200917613620
carotenoids extraction from japanese persimmon (hachiya-kaki) peels by supercritical co(2) with ethanol.the extraction of carotenoids from japanese persimmon peels by supercritical fluid extraction (sfe), of which the solvent was co(2), was performed. in order to enhance the yield and selectivity of the extraction, some portion of ethanol (5 - 20 mol%) was added as an entrainer. the extraction temperature ranged from 313 to 353 k and the pressure was 30 mpa. the effect of temperature on the extraction yield of carotenoids was investigated at 10 mol% of the ethanol concentration in the extraction s ...200617099276
involvement of negative feedback regulation in wound-induced ethylene synthesis in 'saijo' persimmon.wounding is one of the most effective stress signals to induce ethylene synthesis in persimmon (diospyros kaki thunb.). we found that wound-induced ethylene biosynthesis is subjected to negative feedback regulation in mature 'saijo' persimmon fruit since ethylene production was enhanced by 1-methylcyclopropene (1-mcp) (an inhibitor of ethylene perception) pretreatment, which was approximately 1.8 fold of that in control tissues (without 1-mcp pretreatment). wound-induced 1-aminocyclopropane-1-ca ...200616881689
simultaneous quantification of three major bioactive triterpene acids in the leaves of diospyros kaki by high-performance liquid chromatography method.the leaf of diospyros kaki, which is a traditional chinese medicine, has been used for the treatment of various diseases. in order to improve the quality assurance of the leaves of d. kaki, derived extracts and phytomedicines, a simple, rapid and accurate high-performance liquid chromatography (hplc) method was developed to simultaneously assess the three bioactive triterpene acids: barbinervic acid (ba) and its epimer, rotungenic acid (ra), along with 24-hydroxy ursolic acid (ha). this hplc ass ...200616527439
flavonoids from the leaves of diospyros kaki reduce hydrogen peroxide-induced injury of ng108-15 cells.flavonoids from the leaves of diospyros kaki l (fldk-p70) are main therapeutic components of naoxingqing, which is a novel and patented traditional chinese medicine drug used for the treatment of syndrome of apoplexy for years in china. the present study investigated the effects of fldk-p70 on hydrogen peroxide (h2o2)-induced apoptosis-like damage of ng108-15 cells. pretreatment of cells with fldk-p70 alleviated h2o2-induced cell injury and apoptosis by upregulating bcl-2 expression and improvin ...200515707880
kakispyrol, a new biphenyl derivative from the leaves of diospyros kaki.a new biphenyl derivative, 4',5-dimethoxy-3-beta-d-glucopyranosyloxy-4-hydroxy-biphenyl, named kakispyrol (1), has been isolated from the leaves of diospyros kaki, together with three known compounds, vitexin (2), 2'-o-rhamnosyl vitexin (3) and isorhamnetin-3-o-beta-d-glucopyranoside (4). the structure of compound 1 has been determined on the basis of spectroscopic evidence.200515621636
[effects of flavone from leaves of diospyros kaki on adventitial fibroblasts proliferation by advanced oxidation protein products in vitro].to observe the effects of flavone from leaves of diospyros kaki on adventitial fibroblasts proliferation by advanced oxidation protein products (aopp) in vitro.200415272781
structural analysis of a pectic polysaccharide from the leaves of diospyros kaki.a pectic polysaccharide dl-2a with a molar mass of 8.5 x 10(5), was obtained from the boiling water extract of diospyros kaki leaves. it had [alpha]20d -21.8 degrees (c 0.22, h2o) and consisted of rhamnose, arabinose, galactose, xylose and galacturonic acid units in the molar ratio of 0.4:3.4:2.4:1.0:0.8, along with traces of glucuronic acid. about 16.7% of galacturonic acid existed as the methyl ester. a combination of linkage analyses, periodate oxidation, partial acid hydrolysis, selective li ...200415003425
[effects of flavone from leaves of diospyros kaki on adventitial fibroblast proliferation induced by advanced glycation end-products in vitro].to observe the effects of flavone from the leaves of diospyros kaki on proliferation of adventitial fibroblasts induced by advanced glycation end-products (ages) in vitro.200314678884
quantitative magnetic resonance imaging of 'fuyu' persimmon fruit during development and ripening.qualitative and quantitative proton magnetic resonance imaging techniques were applied to persimmon (diospyros kaki cv 'fuyu') fruit during development and post-harvest ripening. spin-lattice (t(1)) relaxation times in mesocarp parenchyma and vascular tissue exhibited a sigmoidal pattern of increase leading to commercial harvest, but declined abruptly during ripening, 2.5 weeks after picking. t(1) times in parenchyma tissue were 1000 and 2100 ms in fruitlets, and at commercial harvest, respectiv ...200312915200
structural features of a pectic arabinogalactan with immunological activity from the leaves of diospyros kaki.a water-soluble acidic heteroglycan, dl-3bb, isolated from the leaves of diospyros kaki, had [alpha](d)(20) -19.9 degrees (c 0.30, water), and contained rhamnose, arabinose, xylose, galactose and galacturonic acid in the molar ratio of 1.0:4.5:0.7:1.5:1.0. about 44% of the galacturonic acid existed as its methyl ester, and o-acetyl groups (approx 5.7%) were also identified. its molecular weight was determined to be 9.0x10(5) da by high-performance gel-permeation chromatography. its structural fe ...200312791282
beta-galactosidase and its significance in ripening of "saijyo" japanese persimmon fruit.the fruit extracts of ripening cv. japanese persimmon, "saijyo", contained a number of glycosidases and glycanases. among them, beta-galactosidase appeared to be the most significant, and the activity increased in parallel with tissue ripening. persimmon beta-galactosidase was presented in at least three isoforms, beta-galactosidase-i (pi = 4.88), beta-galactosidase-ii (pi = 6.76), and beta-galactosidase-iii (pi = 7.05). beta-galactosidase-iii had exo-type galactanase activity, while the others ...200312619675
free radical scavenging effect of diospyros kaki, laminaria japonica and undaria pinnatifida.diospyros kaki folium, laminaria japonica thallus and undaria pinnatifida thallus have been used traditionally in korea to promote maternal health. the scavenging activity against dpph (1,1-diphenyl-2-picrylhydrazyl) radicals of the methanol extracts of these plants were investigated. the extract of d. kaki was found to be the most potent, with an ic(50) value of 0.11 mg/ml.200212490237
ty1-copia group retrotransposons in persimmon (diospyros kaki thunb.).we cloned and characterized ty1-copia group retrotransposons in persimmon (diospyros kaki thunb.). genomic dna or methyl jasmonate (mja)-treated cdna were used as templates to amplify the reverse transcriptase region of ty1-copia group retrotransposons. about 280 bp fragments were amplified and cloned, and 97 clones were sequenced. forty-nine clones included frameshift or the stop codon, or both. among 48 clones containing complete reading frames, 42 clones had unique nucleotide sequences. align ...200212087196
effect of shitei-to, a traditional chinese medicine formulation, on pentylenetetrazol-induced kindling in mice.this study measured the effects of shitei-to (stt), a traditional chinese medicine, which is a mixture of extracts from three medicinal herbs, shitei (si, kaki calyx; calyx of diospyros kaki l. f.), shokyo (sk, zingiberis rhizoma; rhizome of zingiber officinale roscoe) and choji (cj, caryophylli flos; flowerbud of syzygium aromaticum [l.] merrill et. perry), has long been used for the treatment of hiccups in japan and china, against fully pentylenetetrazol-kindled seizures and on the development ...200010782493
production of dodecaploid plants of japanese persimmon (diospyros kaki l.) by colchicine treatment of protoplasts.dodecaploid plants of japanese persimmon (diospyros kaki l.) were obtained by colchicine treatment of protoplasts. callus protoplasts of 'jiro' (2n=90, x=15) were cultured in modified km8p medium with 0.1% colchicine for 3-9 days. after colchicine treatment, they were cultured using agarose bead culture. microcalli were recovered from the protoplasts after 3 months. flow cytometric measurement showed that nine of 31 callus lines obtained from 6 days of colchicine treatment had twice the nuclear ...199624178455
plants with a reputation against snakebite.many plants are recommended in traditional medicine as active against various effects of snakebite. few attempts have been made to investigate the veracity of these assertions in controlled experiments. several workers, mainly oriental, have investigated the reputation of such plants by performing in vitro and in vivo experiments in order to demonstrate whether there was any protective effect, using drugs or mixtures of drugs prepared using traditional formulae. in some studies, these extracts w ...19921440620
novel pathways of oleic and cis-vaccenic acid biosynthesis by an enzymatic double-bond shifting reaction in higher plants.the novel pathways of oleic acid formation from cis-vaccenic (cis-11-octadecenoic) acid and of cis-vaccenic acid formation from oleic acid by enzymatic positional isomerization have been proposed in higher plants, based on stable-isotope experiments using [2,2-2h2]cis-vaccenate or [2,2-2h2]oleate as an immediate precursor. a pulp homogenate and also pulp slices prepared from developing kaki (diospyros kaki) fruit could catalyze these hitherto unknown isomerizations. this suggests the presence of ...19902358068
[studies on the constituents of the useful plants. iv. the constituents of calyx of diospyros kaki. (1) (author's transl)]. 1977559751
[studies on the components of the leaves of diospyros kaki thunb]. 19715165001
[juice from fruits of diospyros kaki]. 20095882998
[vitamin c content in japanese persimmon]. 201313636144
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