| molecular profiling of lactobacillus, streptococcus, and bifidobacterium species in feces of active racehorses. | diversity and compositions of the lactobacillus, streptococcus, and bifidobacterium group in the feces of six healthy, actively racing horses (thoroughbreds) were analyzed by using pcr-denaturing gradient gel electrophoresis (dgge) and real-time pcr with primer sets specific for each group. pcr-dgge analysis of the feces showed that lactobacillus equi, lactobacillus johnsonii, a phylogenetic relative of lactobacillus salivarius, a phylogenetic relative of lactobacillus gastricus, and weissella c ... | 2007 | 17726300 |
| dietary supplementation with sorbitol results in selective enrichment of lactobacilli in rat intestine. | a potential prebiotic action has been ascribed to sorbitol, but in vivo evidence of this remains scarce. in the present work, the effect of sorbitol was compared to that of fructo-oligosaccharides (fos) in a rat model. microbiota changes, particularly in lactobacilli, were analyzed on fecal, colonic and cecal samples. denaturing gradient gel electrophoresis (dgge) analysis of 16s rrna gene amplicons using universal primers showed that fos and sorbitol diets exerted a strong influence upon gut mi ... | 2007 | 17825531 |
| gene expression of commensal lactobacillus johnsonii strain ncc533 during in vitro growth and in the murine gut. | work with pathogens like vibrio cholerae has shown major differences between genes expressed in bacteria grown in vitro and in vivo. to explore this subject for commensals, we investigated the transcription of the lactobacillus johnsonii ncc533 genome during in vitro and in vivo growth using the microarray technology. during broth growth, 537, 626, and 277 of the 1,756 tested genes were expressed during exponential phase, "adaptation" (early stationary phase), and stationary phase, respectively. ... | 2007 | 17827285 |
| enolases from gram-positive bacterial pathogens and commensal lactobacilli share functional similarity in virulence-associated traits. | enolase occurs as a cytoplasmic and a surface-associated protein in bacteria. enolases of the bacterial pathogens streptococcus pyogenes, streptococcus pneumoniae and staphylococcus aureus, as well as of the commensal lactic acid bacteria, lactobacillus crispatus and lactobacillus johnsonii, were purified as his(6)-fusion proteins from recombinant escherichia coli. the fusion proteins were compared for putative virulence-associated functions, i.e., binding of human plasminogen, enhancement of pl ... | 2007 | 17892475 |
| mosaic tetracycline resistance genes and their flanking regions in bifidobacterium thermophilum and lactobacillus johnsonii. | for the first time, mosaic tetracycline resistance genes were identified in lactobacillus johnsonii and in bifidobacterium thermophilum strains. the l. johnsonii strain investigated contains a complex hybrid gene, tet(o/w/32/o/w/o), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(w/32/o) and tet(o/w). as reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for re ... | 2008 | 17967912 |
| bacterial biodiversity of traditional zabady fermented milk. | the aim of this work was to identify the bacterial biodiversity of traditional zabady fermented milk using pcr-temporal temperature gel electrophoresis (pcr-ttge) and pcr-denaturing gradient gel electrophoresis (pcr-dgge). most of the identified bacterial species in zabady samples belonged to lactic acid bacteria (lab), e.g., streptococcus thermophilus, lactococcus garvieae, lactococcus raffinolactis, lactococcus lactis, leuconostoc citreum, lactobacillus delbrueckii subsp. bulgaricus and lactob ... | 2008 | 18077039 |
| glucosyltransferase a (gtfa) and inulosucrase (inu) of lactobacillus reuteri tmw1.106 contribute to cell aggregation, in vitro biofilm formation, and colonization of the mouse gastrointestinal tract. | members of the genus lactobacillus are common inhabitants of the proximal gastrointestinal tract of animals such as mice, rats, chickens and pigs, where they form epithelial biofilms. little is known about the traits that facilitate biofilm formation and gut colonization. this study investigated the ecological role of a glucosyltransferase (gtfa) and inulosucrase (inu) of lactobacillus reuteri tmw1.106 and a fructosyltransferase (ftfa) of l. reuteri lth5448. in vitro experiments using isogenic m ... | 2008 | 18174127 |
| fecal microflora of greek healthy neonates. | this study aimed to explore, in our geographical region, the development of intestinal microflora and the colonization patterns of lactic acid bacteria and bifidobacteria during the first three months of life and to investigate the effect of the mode of delivery. fecal specimens from 82 healthy, full-term infants were collected prospectively 4, 30 and 90 days after delivery and subcultured on nonselective and selective media. identification of isolates was performed by microbiological and molecu ... | 2008 | 18207437 |
| identification of genes associated with the long-gut-persistence phenotype of the probiotic lactobacillus johnsonii strain ncc533 using a combination of genomics and transcriptome analysis. | lactobacillus johnsonii strains ncc533 and atcc 33200 (the type strain of this species) differed significantly in gut residence time (12 versus 5 days) after oral feeding to mice. genes affecting the long gut residence time of the probiotic strain ncc533 were targeted for analysis. we hypothesized that genes specific for this strain, which are expressed during passage of the bacterium through the gut, affect the phenotype. when the dna of the type strain was hybridized against a microarray of th ... | 2008 | 18223069 |
| modulation of helicobacter pylori colonization with cranberry juice and lactobacillus johnsonii la1 in children. | probiotics and cranberry have been shown to inhibit helicobacter pylori in vitro owing to bacteriocin production and high levels of proanthocyanidins, respectively. these effects have been confirmed in clinical trials with h. pylori-positive subjects. the aim of this study was to evaluate whether regular intake of cranberry juice and the probiotic lactobacillus johnsonii la1 (la1) may result in an additive or synergistic inhibition of h. pylori in colonized children. | 2008 | 18343637 |
| the probiotic lactobacillus johnsonii ncc 533 produces high-molecular-mass inulin from sucrose by using an inulosucrase enzyme. | fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with beta(2-6) and beta(2-1) linkages, respectively. the probiotic bacterium lactobacillus johnsonii strain ncc 533 possesses a single fructansucrase gene (open reading frame aas08734) annotated as a putative levansucrase precursor. however, (13)c nuclear magnetic resonance (nmr) analysis of the fructan product synthesized in situ revealed that this is of the inulin type. the ftf gene of l. johnsonii ... | 2008 | 18408060 |
| prophylactic effect of oral administration of lactobacillus johnsonii ncc533 (la1) during the weaning period on atopic dermatitis in nc/ngatnd mice. | bacterial exposure in infancy may be one of the determinants of atopic dermatitis (ad) morbidity in later life. some clinical studies have shown that an intake of probiotics reduced the risks of ad in children; however, the timing and duration of administration for the prevention of ad still remain unclear. the aim of this study was to evaluate the effects on ad development of the administration of lactobacillus johnsonii nc553 (la1) during the weaning period, using an animal model of human ad, ... | 2008 | 18424371 |
| yoghurts containing probiotics reduce disruption of the small intestinal barrier in methotrexate-treated rats. | small intestinal permeability was employed to assess the efficacy of commercially available yoghurts containing probiotics in a rat model of methotrexate (mtx)-induced mucositis. male sprague-dawley rats were allocated to four groups (n = 8): mtx + water, mtx + cow's milk yoghurt (cy; fermented with lactobacillus johnsonii), mtx + sheep's milk yoghurt (sy; containing lactobacillus bulgaricus and streptococcus thermophilus), and saline. treatment gavage occurred twice daily for 7 days pre-mtx and ... | 2008 | 18427990 |
| hydrogen peroxide production by lactobacillus johnsonii ncc 533 and its role in anti-salmonella activity. | the human intestinal isolate lactobacillus johnsonii ncc 533 (la1) is a probiotic strain with well-documented antimicrobial properties. previous research has identified the production of lactic acid and bacteriocins as important factors, but that other unidentified factors are also involved. we used the recently published genome sequence of l. johnsonii ncc 533 to search for novel antipathogen factors and identified three potential gene products that may catalyze the synthesis of the known antim ... | 2008 | 18435747 |
| the role of prophage for genome diversification within a clonal lineage of lactobacillus johnsonii: characterization of the defective prophage lj771. | two independent isolates of the gut commensal lactobacillus johnsonii were sequenced. these isolates belonged to the same clonal lineage and differed mainly by a 40.8-kb prophage, lj771, belonging to the sfi11 phage lineage. lj771 shares close dna sequence identity with lactobacillus gasseri prophages. lj771 coexists as an integrated prophage and excised circular phage dna, but phage dna packaged into extracellular phage particles was not detected. between the phage lysin gene and attr a likely ... | 2008 | 18515417 |
| lactobacillus johnsonii provides a dose-dependent protection against uvr-induced immunosuppression. | | 2008 | 18573738 |
| single bioreactor gastrointestinal tract simulator for study of survival of probiotic bacteria. | the aim of the present study was to design an in vitro model system to evaluate the probiotic potential of food. a single bioreactor system-gastrointestinal tract simulator (gits) was chosen for process simulation on account of its considerable simplicity compared to multi-vessel systems used in previous studies. the bioreactor was evaluated by studying the viability of four known probiotic bacteria (lactobacillus acidophilus la-5, lactobacillus johnsonii ncc 533, lactobacillus casei strain shir ... | 2008 | 18581109 |
| complete nucleotide sequence of plasmid plca36 isolated from lactobacillus casei zhang. | the complete 36,487 bp sequence of plasmid plca36 from lactobacillus casei zhang was determined. plca36 contains 44 predicted coding regions, and to 23 of them functions could be assigned. for the first time, we identified a relbe toxin-antitoxin (ta) locus in a lactobacillus genus, perhaps indicating a potential role for plca36 in host survival under extreme nutritional stress. a region encoding a cluster of conjugation genes (tra) was also identified. the cluster showed high similarity and co- ... | 2008 | 18634821 |
| supplementation with oral probiotic bacteria protects human cutaneous immune homeostasis after uv exposure-double blind, randomized, placebo controlled clinical trial. | there is now strong evidence that probiotic bacteria can regulate inflammatory immune responses. here, we analyzed whether oral supplementation with the probiotic bacterial strain lactobacillus johnsonii (la1) could interfere with skin immune status following uv exposure. a randomized, double-blind, placebo controlled clinical trial was conducted with 54 healthy volunteers receiving either la1 or placebo, during six weeks prior to solar-simulated uv irradiation. blister roofs and skin biopsies w ... | 2008 | 18693151 |
| characterization of the amylovorin locus of lactobacillus amylovorus dce 471, producer of a bacteriocin active against pseudomonas aeruginosa, in combination with colistin and pyocins. | lactobacillus amylovorus dce 471 produces amylovorin l, a bacteriocin with an antibacterial activity against some strains of the lactobacillus lineage. based on the sequence of one active peptide, a gene encoding active amylovorin l was cloned and sequenced. genome walking allowed us to sequence a larger fragment of 7577 bp of genomic dna, with 12 predicted orfs. the previously characterized amylovorin l peptide-encoding gene is preceded by another gene encoding a small polypeptide with a typica ... | 2008 | 18803001 |
| prior stimulation of antigen-presenting cells with lactobacillus regulates excessive antigen-specific cytokine responses in vitro when compared with bacteroides. | the development of allergy is related to differences in the intestinal microbiota. therefore, it is suggested that the immune responses induced by different genera of bacteria might be regulated through adaptive as well as innate immunity. in this study, we examined whether antigen-specific immune responses were affected by stimulation with the different genera of intestinal bacteria in vitro. mesenteric lymph node (mln) cells isolated from germ-free ovalbumin (ova)-specific t cell receptor tran ... | 2007 | 19002998 |
| urinary d-lactate excretion in infants receiving lactobacillus johnsonii with formula. | supplementation with certain probiotics can improve gut microbial flora and immune function but should not have adverse effects. this study aimed to assess the risk of d-lactate accumulation and subsequent metabolic acidosis in infants fed on formula containing lactobacillus johnsonii (la1). | 2008 | 19088469 |
| indigestible carbohydrates alter the intestinal microbiota but do not influence the performance of broiler chickens. | prebiotics are a potential alternative to in-feed antimicrobials to improve performance of chickens. we investigated the effects of mannanoligosaccharide (mos) and fructooligosaccharide (fos) on growth, performance and the intestinal microbiota. | 2009 | 19187131 |
| meta-analysis: the effect and adverse events of lactobacilli versus placebo in maintenance therapy for crohn disease. | lactobacilli are used in an attempt to maintain remission for crohn disease. the aim of this study was to evaluate the efficacy and adverse events of lactobacilli compared with placebo in maintenance therapy for crohn disease. | 2009 | 19220543 |
| distribution of receptor glycolipids for lactobacilli in murine digestive tract and production of antibodies cross-reactive with them by immunization of rabbits with lactobacilli. | in the digestive tract of mice (hr-1 strain), glycolipids belonging to the ganglio-series were revealed to be expressed in region-specific manners, i.e. fga1 and fgm1 in the stomach, ga1 in the small intestine, and fga1 and sulphatides in the cecum. the amount of ga1 as a receptor glycolipid for lactobacilli was especially higher in the small intestine than in the other regions, it comprising 1.6-2.8 microg/mg dry weight. on immunization of rabbits with lactobacillus johnsonii and lactobacillus ... | 2009 | 19351707 |
| a chinese rhesus macaque (macaca mulatta) model for vaginal lactobacillus colonization and live microbicide development. | we sought to establish a nonhuman primate model of vaginal lactobacillus colonization suitable for evaluating live microbial microbicide candidates. | 2009 | 19367737 |
| spr1630 is responsible for the lethality of clpx mutations in streptococcus pneumoniae. | the clp protease atpase subunit and chaperone clpx is dispensable in some bacteria, but it is thought to be essential in others, including streptococci and lactococci. we confirm that clpx is essential in the rx strain of streptococcus pneumoniae but show that the requirement for clpx can be relieved by point mutations, frame shifts, or deletion of the gene spr1630, which is found in many isolates of s. pneumoniae. homologs occur frequently in staphylococcus aureus as well as in a few strains of ... | 2009 | 19465654 |
| biochemical properties of two cinnamoyl esterases purified from a lactobacillus johnsonii strain isolated from stool samples of diabetes-resistant rats. | cinnamic acids (i.e., ferulic and caffeic acids) that are esterified to the vegetable cell walls should be enzymatically released to be absorbed in a mammal's intestines. a low dosage of ferulic acid in rodent diets stimulates insulin production and alleviates symptoms caused by diabetes (m. sri balasubashini, r. rukkumani, and v. p. menon, acta diabetol. 40:118-122, 2003). several lactic acid bacteria are able to display ferulic acid esterase (fae) activity, suggesting that their probiotic acti ... | 2009 | 19502437 |
| a mesocosm of lactobacillus johnsonii, bifidobacterium longum, and escherichia coli in the mouse gut. | the relative contribution of competition and cooperation at the microbe-microbe level is not well understood for the bacteria constituting the gut microbiota. the high number and variability of human gut commensals have hampered the analysis. to get some insight into the question how so many different bacterial species can coexist in the mammalian gut, we studied the interaction between three human gut commensals (escherichia coli k-12, lactobacillus johnsonii ncc533, and bifidobacterium longum ... | 2009 | 19534605 |
| lactobacillus taiwanensis sp. nov., isolated from silage. | strain firdi 006t, isolated from silage cattle feed, was characterized by using phenotypic and molecular taxonomic methods. the strain was a homofermentative lactic acid bacterium; the cells stained gram-positive and were catalase-negative, non-motile, facultatively anaerobic rods. 16s rrna gene sequence analysis revealed that the strain belongs phylogenetically to the genus lactobacillus and can be placed within the lactobacillus acidophilus-delbrueckii group. lactobacillus gasseri and lactobac ... | 2009 | 19605711 |
| alteration of the ileal microbiota of weanling piglets by the growth-promoting antibiotic chlortetracycline. | antibiotics such as chlortetracycline (ctc) have been used to promote growth of pigs for decades, but concerns over increased antibiotic-resistant infections in humans have prompted the development of alternative strategies. developing alternatives to antibiotic growth promoters (agps) could be informed by information on the mechanisms of growth promotion, notably, how agps affect the microbial populations of the gastrointestinal tract. pigs from three sows were aseptically delivered by cesarean ... | 2009 | 19617391 |
| hydrolysis of rosmarinic acid from rosemary extract with esterases and lactobacillus johnsonii in vitro and in a gastrointestinal model. | rosmarinic acid (ra) was identified as one of the main components of rosemary extracts and has been ascribed to a number of health benefits. several studies suggested that after ingestion, ra is metabolized by gut microflora into caffeic acid and derivatives. however, only limited information on the microorganisms and enzymes involved in this biotransformation is available. in this study, we investigated the hydrolysis of ra from rosemary extract with enzymes and a probiotic bacterium lactobacil ... | 2009 | 19658402 |
| enhancement of survival of probiotic and non-probiotic lactic acid bacteria by yeasts in fermented milk under non-refrigerated conditions. | the effects of yeasts on the survival of probiotic and non-probiotic lactic acid bacteria (lab) were studied in fermented milk under non-refrigerated conditions (30 degrees c) with a view to develop ambient-stable fermented milk with live lab. five yeasts tested (saccharomyces bayanus, williopsis saturnus var. saturnus, yarrowia lipolytica, candida kefyr and kluyveromyces marxianus) enhanced the survival of lactobacillus bulgaricus (but not streptococcus thermophilus) in a mixed yoghurt culture ... | 2009 | 19666198 |
| the first alpha-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 31. | genome analysis of lactobacillus johnsonii ncc533 has been recently completed. one of its annotated genes, lj0569, encodes the protein having the conserved domain of glycoside hydrolase family 31. its homolog gene (ljag31) in l. johnsonii nbrc13952 was cloned and expressed using an escherichia coli expression system, resulting in poor production of recombinant ljag31 protein due to inclusion body formation. production of soluble recombinant ljag31 was improved with high concentration of nacl in ... | 2009 | 19683032 |
| lactobacillus and bifidobacterium diversity in horse feces, revealed by pcr-dgge. | lactobacillus equi, lactobacillus hayakitensis, lactobacillus johnsonii, and weissella confusa/cibaria were the dominant species in 12 south african horses. the bifidobacterium-group was detected in the feces of only one of the 12 horses. sequencing of the nested-pcr amplicon identified the bifidobacterium-group as parascardovia denticolens. cell numbers of l. equi, l. hayakitensis, and w. confusa/cibaria were consistent in all samples. p. denticolens, bifidodobacterium pseudolongum, and a phylo ... | 2009 | 19730939 |
| peptidoglycan from lactobacilli inhibits interleukin-12 production by macrophages induced by lactobacillus casei through toll-like receptor 2-dependent and independent mechanisms. | we previously showed that lactobacillus strains having a rigid cell wall resistant to intracellular digestion can stimulate macrophages to induce large a quantity of interleukin-12 (il-12). in this study, we examined the influence of lactobacilli and bacterial cell wall components on il-12 production by macrophages that was induced by lactobacillus casei, which has a rigid cell wall. easily digestible lactobacilli such as lactobacillus johnsonii and lactobacillus plantarum or their intact cell w ... | 2009 | 19740347 |
| comparison and utilization of repetitive-element pcr techniques for typing lactobacillus isolates from the chicken gastrointestinal tract. | three repetitive-element pcr techniques were evaluated for the ability to type strains of lactobacillus species commonly identified in the chicken gastrointestinal tract. enterobacterial repetitive intergenic consensus pcr (eric-pcr) produced species- and strain-specific profiles for lactobacillus crispatus, lactobacillus gallinarum, lactobacillus johnsonii, and lactobacillus reuteri isolates. the technique typed strains within these species equally as well as pulsed-field gel electrophoresis. d ... | 2009 | 19749057 |
| complete genome sequence of lactobacillus johnsonii fi9785, a competitive exclusion agent against pathogens in poultry. | lactobacillus johnsonii is a member of the acidophilus group of lactobacilli. because of their probiotic properties, including attachment to epithelial cells, immunomodulation, and competitive exclusion of pathogens, representatives of this group are being intensively studied. here we report the complete annotated genome sequence of lactobacillus johnsonii fi9785, a strain which prevents the colonization of specific-pathogen-free chicks by clostridium perfringens. | 2009 | 19767436 |
| reconstitution conditions for dried probiotic powders represent a critical step in determining cell viability. | resuscitation of dried cultures represents a critical control point in obtaining active and effective probiotic strains. this study examined the effects of various rehydration conditions on the viability of bifidobacterium longum ncc3001 and lactobacillus johnsonii la1. | 2010 | 19796125 |
| structural and immunochemical studies of neutral exopolysaccharide produced by lactobacillus johnsonii 142. | this paper describes the structure of neutral exopolysaccharide (eps) produced by lactobacillus johnsonii 142, strain of the lactic acid bacteria isolated from the intestine of mice with experimentally induced inflammatory bowel disease (ibd). sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including two-dimensional (1)h,(1)h cosy, tocsy, noesy, and (1)h,(13)c hsqc experiments revealed that the repeating unit of the eps is a pentasaccharide: -->3)-alpha-d-galp-(1-->3)- ... | 2010 | 19897181 |
| a randomized double-blind trial on perioperative administration of probiotics in colorectal cancer patients. | to investigate whether probiotic bacteria, given perioperatively, might adhere to the colonic mucosa, reduce concentration of pathogens in stools, and modulate the local immune function. | 2010 | 20066735 |
| individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain lactobacillus johnsonii ncc933 and vaginal strain lactobacillus gasseri ks120.1 against enteric, uropathogenic and vaginosis-associated pathogens. | the mechanism underlying the killing activity of lactobacillus strains against bacterial pathogens appears to be multifactorial. here, we investigate the respective contributions of hydrogen peroxide and lactic acid in killing bacterial pathogens associated with the human vagina, urinary tract or intestine by two hydrogen peroxide-producing strains. in co-culture, the human intestinal strain lactobacillus johnsonii ncc933 and human vaginal strain lactobacillus gasseri ks120.1 strains killed ente ... | 2010 | 20082639 |
| properties of different lactic acid bacteria isolated from apis mellifera l. bee-gut. | eight strains belonging to lactobacillus spp. and five to enterococcus spp. were isolated from the gut of worker apis mellifera l. bees. studies based on 16s rrna sequencing revealed that aj5, ig9, a15 and crl1647 strains had a 99% identity with lactobacillus johnsonii, while sm21 showed a 99% similarity with enterococcus faecium. l. johnsonii crl1647, aj5 and ig9 were high lactic acid producers (values were between 177 and 275 mm), and in vitro they inhibited different human food-borne pathogen ... | 2011 | 20116222 |
| lactobacillus johnsonii n6.2 mitigates the development of type 1 diabetes in bb-dp rats. | background: the intestinal epithelium is a barrier that composes one of the most immunologically active surfaces of the body due to constant exposure to microorganisms as well as an infinite diversity of food antigens. disruption of intestinal barrier function and aberrant mucosal immune activation have been implicated in a variety of diseases within and outside of the gastrointestinal tract. with this model in mind, recent studies have shown a link between diet, composition of intestinal microb ... | 2010 | 20463897 |
| comparison of alternatives to in-feed antimicrobials for the prevention of clinical necrotic enteritis. | the capacity for lactobacillus johnsonii and an organic acid (oa) blend to prevent clostridium perfringens-induced clinical necrotic enteritis (ne) in chickens was studied. | 2010 | 20497278 |
| clinical evidence of benefits of a dietary supplement containing probiotic and carotenoids on ultraviolet-induced skin damage. | lactobacillus johnsonii (la1) has been reported to protect skin immune system homeostasis following ultraviolet (uv) exposure. | 2010 | 20545689 |
| probiotics for photoprotection. | specific strains of probiotic, have been identified as beneficial to influence the composition and/or metabolic activity of the endogenous microbiota and some of these strains have been also shown to inhibit the growth of a wide range of enteropathogens. the first aim of using probiotics has been to improve the composition of the intestinal microbiota from a potentially harmful composition towards a composition that would be beneficial to the host.beyond their capacity to influence positively th ... | 2009 | 20808516 |
| dying in yoghurt: the number of living bacteria in probiotic yoghurt decreases under exposure to room temperature. | while probiotic bacteria are successfully used in the treatment of ulcerative colitis, the effect of commercially available probiotic products is still controversial. here, we study whether the number of living probiotic bacteria in yoghurts is altered by an interruption of the cold chain. | 2011 | 20838050 |
| characterization of a novel lactobacillus species closely related to lactobacillus johnsonii using a combination of molecular and comparative genomics methods. | comparative genomic hybridization (cgh) constitutes a powerful tool for identification and characterization of bacterial strains. in this study we have applied this technique for the characterization of a number of lactobacillus strains isolated from the intestinal content of rats fed with a diet supplemented with sorbitol. | 2010 | 20849602 |
| diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores. | the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ... | 2010 | 21034840 |
| efficacy of combined or single use of lactobacillus crispatus lt116 and l. johnsonii lt171 on broiler performance. | 1. the objective of this research was to investigate the efficacy of combined or single use of lactobacillus crispatus lt116 and lactobacillus johnsonii lt171 on broiler performance. 2. a total of 320 one-d-old male ross broiler chicks were allocated in 4 experimental treatments for 6 weeks. the experimental treatments received a maize-soybean meal basal diet that was supplemented as follows: 'control', with no other additions; 'lj', 1 × 10(6) cfu of l. johnsonii lt171; 'lc', 1 × 10(6) cfu of l. ... | 2010 | 21058059 |
| excretion into feces of asialo gm1 in the murine digestive tract and lactobacillus johnsonii exhibiting binding ability toward asialo gm1. a possible role of epithelial glycolipids in the discharge of intestinal bacteria. | in the digestive tract of mice (hr-1, 5 months old, ♀), asialo gm1 (ga1) exhibiting receptor activity toward several intestinal bacteria was preferentially expressed in the small intestine. also, less than 10% of ga1 in the small intestine was converted into fucosylated and sulfated derivatives, but it was completely converted to fucosyl ga1 (fga1) in the stomach, cecum and colon. among the lipid components in these tissues, glycolipids other than forssman antigen and cholesterol sulfate (cs) we ... | 2010 | 21174147 |
| inhibition of type 1 diabetes correlated to a lactobacillus johnsonii n6.2-mediated th17 bias. | although it is known that resident gut flora contribute to immune system function and homeostasis, their role in the progression of the autoimmune disease type 1 diabetes (t1d) is poorly understood. comparison of stool samples isolated from bio-breeding rats, a classic model of t1d, shows that distinct bacterial populations reside in spontaneous bio-breeding diabetes-prone (bbdp) and bio-breeding diabetes-resistant animals. we have previously shown that the oral transfer of lactobacillus johnson ... | 2011 | 21317395 |
| an in vitro protocol for direct isolation of potential probiotic lactobacilli from raw bovine milk and traditional fermented milks. | a method for isolating potential probiotic lactobacilli directly from traditional milk-based foods was developed. the novel digestion/enrichment protocol was set up taking care to minimize the protective effect of milk proteins and fats and was validated testing three commercial fermented milks containing well-known probiotic lactobacillus strains. only probiotic bacteria claimed in the label were isolated from two out of three commercial fermented milks. the application of the new protocol to 1 ... | 2011 | 21318359 |
| impact of ecological factors on the stability of microbial associations in sourdough fermentation. | the limits for the stability of the microbial association 1 (lactobacillus sanfranciscensis and candida humilis) and association 2 (lactobacillus reuteri, lactobacillus johnsonii and issatchenkia orientalis) during sourdough fermentation were evaluated by investigating the effects of the ecological factors substrate, refreshment time, temperature, amount of backslopping and competing species in different combinations on their growth. sourdoughs were fermented in 28 batches under different condit ... | 2010 | 21356468 |
| klebsiella pneumoniae increases the risk of inflammation and colitis in a murine model of intestinal bowel disease. | intrarectal treatment with 2,4,6-trinitrobenzene sulfonic acid (tnbs), an inducer of intestinal inflammation, in mice increases the population size of enterobacteriaceae, particularly klebsiella pneumoniae, while reducing the population size of lactic acid bacteria. therefore, we investigated the effects of these bacteria in tnbs-induced colitis in mice. | 2011 | 21410316 |
| influence of antimicrobial feed additives on broiler commensal posthatch gut microbiota development and performance. | the effects of avilamycin, zinc bacitracin, and flavophospholipol on broiler gut microbial community colonization and bird performance in the first 17 days posthatch were investigated. significant differences in gut microbiota associated with gut section, dietary treatment, and age were identified by terminal restriction fragment length polymorphism (t-rflp), although no performance-related differences between dietary treatments were detected. similar age-related shifts in the gut microbiota wer ... | 2011 | 21441326 |
| group-specific comparison of four lactobacilli isolated from human sources using differential blast analysis. | lactic acid bacteria (lab) have been used in fermentation processes for centuries. more recent applications including the use of lab as probiotics have significantly increased industrial interest. here we present a comparative genomic analysis of four completely sequenced lactobacillus strains, isolated from the human gastrointestinal tract, versus 25 lactic acid bacterial genomes present in the public database at the time of analysis. lactobacillus acidophilus ncfm, lactobacillus johnsonii ncc5 ... | 2010 | 21484153 |
| lactobacillus johnsonii n6.2 stimulates the innate immune response through toll-like receptor 9 in caco-2 cells and increases intestinal crypt paneth cell number in biobreeding diabetes-prone rats. | lactobacillus johnsonii (ljo) n6.2 has been shown to mitigate the development of type 1 diabetes when administered to diabetes-prone rats. the specific mechanisms underlying this observed response remain under investigation. the objective of this study was to assess the effect of ljo n6.2 on mucosal inflammatory response using differentiated caco-2 monolayers. the mrna expression levels of ccl20, cxcl8, and cxcl10 chemokines were determined by qrt-pcr. ljo at 10(11) cfu/l induced a strong respon ... | 2011 | 21490291 |
| host specific diversity in lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms. | genetic diversity and genomic rearrangements are a driving force in bacterial evolution and niche adaptation. we sequenced and annotated the genome of lactobacillus johnsonii dpc6026, a strain isolated from the porcine intestinal tract. although the genome of dpc6026 is similar in size (1.97mbp) and gc content (34.8%) to the sequenced human isolate l. johnsonii ncc 533, a large symmetrical inversion of approximately 750 kb differentiated the two strains. comparative analysis among 12 other strai ... | 2011 | 21533100 |
| the effect of lactobacillus johnsonii ncc533 (la1) on the balance of th1/th2 cells in balb/c mice. | purpose: to determine the effect of lactobacillus johnsonii ncc533 (la1) on th1/th2 balance, the production of il-4 and ifn-? by splenocytes was evaluated following its administration to mice from newborn to adult. changes in il-4 and ifn-? expression and serum levels of ova-specific-ige were then investigated in an asthma model. methods: using flow cytometry (fcm) and elisa, the percentage of il-4 and ifn-? expressing splenocytes and serum levels of ova-specific-ige were measured in different g ... | 2011 | 21810385 |
| predominant effect of host genetics on levels of lactobacillus johnsonii in mouse gut. | the gut microbiota is strongly associated with the well-being of the host. its composition is affected by environmental factors, such as food and maternal inoculation while the relative impact of the host's genetics is recently uncovered. here we studied the effect of host genetic background on the composition of intestinal bacteria in a murine model, focusing on lactic acid bacteria (lab) as an important group that includes many probiotic strains. based on 16s-rdna genotyping, variation was obs ... | 2011 | 21803912 |
| crystal structure of inulosucrase from lactobacillus: insights into the substrate specificity and product specificity of gh68 fructansucrases. | fructansucrases (fss) catalyze a transfructosylation reaction with sucrose as substrate to produce fructo-oligosaccharides and fructan polymers that contain either ß-2,1 glycosidic linkages (inulin) or ß-2,6 linkages (levan). levan-synthesizing fss (levansucrases) have been most extensively investigated, while detailed information on inulosucrases is limited. importantly, the molecular basis of the different product specificities of levansucrases and inulosucrases is poorly understood. we have e ... | 2011 | 21801732 |
| lactobacillus johnsonii hy7042 ameliorates gardnerella vaginalis-induced vaginosis by killing gardnerella vaginalis and inhibiting nf-?b activation. | hydrogen peroxide-producing lactic acid bacteria (lab) were isolated from women's vaginas and their anti-inflammatory effects against gardnerella vaginalis-induced vaginosis were examined in ß-estradiol-immunosuppressed mice. oral and intravaginal treatment with five labs significantly decreased viable g. vaginalis numbers in vaginal cavities and myeloperoxidase activity in mouse vaginal tissues. of the labs examined, lactobacillus johnsonii hy7042 (lj) most potently inhibited g. vaginalis-induc ... | 2011 | 21798373 |
| characterization of novel glycolipid antigens with an {alpha}-galactose epitope in lactobacilli detected with rabbit anti-lactobacillus antisera and occurrence of antibodies against them in human sera. | anti-lactobacillus johnsonii (lj) antisera generated by immunization of rabbits with lj reacted with glyceroglycolipids in lj, i.e. dihexaosyl diacylglycerol (dh-dg), trihexaosyl dg (th-dg), and tetrahexaosyl dg (teth-dg), whose reactivities with antisera increased proportionally with longer carbohydrate chains of glycolipids. structural analyses of glycolipids from lj revealed that dh-dg was gala1-2glca1-3'dg, and th-dg and teth-dg were novel derivatives of it with a-gal at the nonreducing term ... | 2011 | 21784785 |
| lactobacillus johnsonii crl1647, isolated from apis mellifera l. bee-gut, exhibited a beneficial effect on honeybee colonies. | lactobacillus johnsonii crl1647, isolated from the intestinal tract of a honeybee and selected due to its high lactic acid production, was assayed as a monoculture on bee colony performance. it was delivered to the bees on a one litre of 125 g/l sugar-cane syrup with a final concentration of 105 cfu/ml lactobacilli. the bees accepted the new nourishment, which was consumed within 24-48 h and was administered in two independent trials (every 14-15 days for 3 consecutive months in one case, and on ... | 2011 | 21831787 |
| modification of technical properties of lactobacillus johnsonii ncc 533 by supplementing growth medium with unsaturated fatty acids. | the aim of this study was to investigate the influence of supplementing growth media with unsaturated fatty acids on the technical properties of the probiotic strain lactobacillus johnsonii ncc 533, such as heat and acid tolerance, and inhibition of salmonella typhimurium infection. our results showed that the membrane composition and morphology of l. johnsonii ncc 533 was significantly changed by supplementing a minimal lactobacillus media with oleic, linoleic and linolenic acids. the ratio of ... | 2011 | 21821758 |
| genome sequence of lactobacillus johnsonii pf01, isolated from piglet feces. | lactobacillus johnsonii pf01, an autochthonous bacterium of the gastrointestinal tract, was isolated from a fecal sample from a piglet. the strain adhered specifically to the duodenal and jejunal epithelial cells of the piglet and had high bile resistance activity. here we report the genomic sequence of l. johnsonii pf01. | 2011 | 21742886 |
| lactic acid bacteria from chicken carcasses with inhibitory activity against salmonella spp. and listeria monocytogenes. | this study was conducted to isolate psychrotrophic lactic acid bacteria (lab) from chicken carcasses with inhibitory activity against strains of salmonella spp. and listeria monocytogenes. a total of 100 broiler samples were examined for the presence of lab. ninety-two lab isolates that showed antimicrobial effects against salmonella spp. and l. monocytogenes were further analysed to examine their lab (gram-positive, catalase negative, oxidase negative) and psychrotrophic characteristics (abilit ... | 2011 | 21983108 |
| An inserted a/ß subdomain shapes the catalytic pocket of Lactobacillus johnsonii cinnamoyl esterase. | Microbial enzymes produced in the gastrointestinal tract are primarily responsible for the release and biochemical transformation of absorbable bioactive monophenols. In the present work we described the crystal structure of LJ0536, a serine cinnamoyl esterase produced by the probiotic bacterium Lactobacillus johnsonii N6.2. | 2011 | 21876742 |
| resource partitioning in relation to cohabitation of lactobacillus species in the mouse forestomach. | phylogenetic analysis of gut communities of vertebrates is advanced, but the relationships, especially at the trophic level, between commensals that share gut habitats of monogastric animals have not been investigated to any extent. lactobacillus reuteri strain 100-23 and lactobacillus johnsonii strain 100-33 cohabit in the forestomach of mice. according to the niche exclusion principle, this should not be possible because both strains can utilise the two main fermentable carbohydrates present i ... | 2011 | 22094343 |
| Cloning and expression of a gene with phospholipase B activity from Pseudomonas fluorescens in Escherichia coli. | A gene from Pseudomonasfluorescens BIT-18 encoding a protein with phospholipase B activity (Pf-PLB) was cloned in E. coli BL21 (DE3). The open reading frame consists of 1272bp and potentially encodes a protein of 423 amino acid residues with a calculated molecular mass of 45.8kDa. The nucleotide sequence of Pf-PLB is 45%, 42%, 41%, 40%, 33%, and 31% identical to that of Bifidobacteriumanimals, Mycobacteriumparascrofulaceum, Acidobacteriumcapsulatum, Lactobacillusjohnsonii, Moraxellabovis, and Mo ... | 2012 | 22078969 |
| mrna profiling for vaginal fluid and menstrual blood identification. | the detection and identification of human biological fluids, including vaginal secretions and menstrual blood, are highly important in forensic biology. previous studies have proposed a few mrna and bacterial markers for vaginal fluid detection, but they have not proven to be specific and reliable. the aim of this project was to develop, validate and evaluate a reliable, specific test for vaginal fluid identification that would combine detection of vaginal mrnas and lactobacilli. we have develop ... | 2012 | 23273817 |
| estdz3: a new esterolytic enzyme exhibiting remarkable thermostability. | lipolytic enzymes that retain high levels of catalytic activity when exposed to a variety of denaturing conditions are of high importance for a number of biotechnological applications. in this study, we aimed to identify new lipolytic enzymes, which are highly resistant to prolonged exposure to elevated temperatures. to achieve this, we searched for genes encoding for such proteins in the genomes of a microbial consortium residing in a hot spring located in china. after performing functional gen ... | 2016 | 27899916 |
| h2o2 production rate in lactobacillus johnsonii is modulated via the interplay of a heterodimeric flavin oxidoreductase with a soluble 28 kd pas domain containing protein. | host and commensals crosstalk, mediated by reactive oxygen species (ros), has triggered a growing scientific interest to understand the mechanisms governing such interaction. however, the majority of the scientific studies published do not evaluate the ros production by commensals bacteria. in this context we recently showed that lactobacillus johnsonii n6.2, a strain of probiotic value, modulates the activity of the critical enzymes 2,3-indoleamine dioxygenase via h2o2 production. l. johnsonii ... | 2015 | 26236298 |
| h(2)o(2) production in species of the lactobacillus acidophilus group: a central role for a novel nadh-dependent flavin reductase. | hydrogen peroxide production is a well-known trait of many bacterial species associated with the human body. in the presence of oxygen, the probiotic lactic acid bacterium lactobacillus johnsonii ncc 533 excretes up to 1 mm h(2)o(2), inducing growth stagnation and cell death. disruption of genes commonly assumed to be involved in h(2)o(2) production (e.g., pyruvate oxidase, nadh oxidase, and lactate oxidase) did not affect this. here we describe the purification of a novel nadh-dependent flavin ... | 2014 | 24487531 |
| biotransformation of caffeoyl quinic acids from green coffee extracts by lactobacillus johnsonii ncc 533. | the potential of lactobacillus johnsonii ncc 533 to metabolize chlorogenic acids from green coffee extract was investigated. two enzymes, an esterase and a hydroxycinnamate decarboxylase (hcd), were involved in this biotransformation. the complete hydrolysis of 5-caffeoylquinic acid (5-cqa) into caffeic acid (ca) by l. johnsonii esterase occurred during the first 16 h of reaction time. no dihydrocaffeic acid was identified in the reaction mixture. the decarboxylation of ca into 4-vinylcatechol ( ... | 2013 | 23692950 |
| molecular analysis and clinical significance of lactobacillus spp. recovered from clinical specimens presumptively associated with disease. | lactobacillus spp. are part of the normal human flora and are generally assumed to be nonpathogenic. we determined the genotypic identification of >100 lactobacillus isolates from clinical specimens in the context of presumed pathogenic potential (e.g., recovered as the single/predominant isolate from a sterile site or at ≥10(5) cfu/ml from urine). this study assessed the clinical significance and the frequency of occurrence of each lactobacillus sp. we identified 16 species of lactobacillus by ... | 2014 | 24131686 |
| inhibition of bacillus cereus strains by antimicrobial metabolites from lactobacillus johnsonii crl1647 and enterococcus faecium sm21. | bacillus cereus is an endospore-forming, gram-positive bacterium able to cause foodborne diseases. lactic acid bacteria (lab) are known for their ability to synthesize organic acids and bacteriocins, but the potential of these compounds against b. cereus has been scarcely documented in food models. the present study has examined the effect of the metabolites produced by lactobacillus johnsonii crl1647 and enterococcus faecium sm21 on the viability of select b. cereus strains. furthermore, the ef ... | 2014 | 25305011 |
| gram-positive bacteria with probiotic potential for the apis mellifera l. honey bee: the experience in the northwest of argentina. | apis mellifera l. is one of the most important natural pollinators of significant crops and flowers around the world. it can be affected by different types of illnesses: american foulbrood, nosemosis, varroasis, viruses, among others. such infections mainly cause a reduction in honey production and in extreme situations, the death of the colony. argentina is the world's second largest honey exporter and the third largest honey producer, after china and turkey. given both the prominence of the ho ... | 2017 | 27655068 |
| the role of conserved inulosucrase residues in the reaction and product specificity of lactobacillus reuteri inulosucrase. | the probiotic bacterium lactobacillus reuteri 121 produces two fructosyltransferase enzymes, a levansucrase and an inulosucrase. although these two fructosyltransferase enzymes share high sequence similarity, they differ significantly in the type and size distribution of fructooligosaccharide products synthesized from sucrose, and in their activity levels. in order to examine the contribution of specific amino acids to such differences, 15 single and four multiple inulosucrase mutants were desig ... | 2012 | 22823473 |
| influence and effect of the human microbiome in allergy and asthma. | studies have illustrated that the healthy human microbiome (i.e. the communities of microbes, their genomic content and interaction with the host) plays a role in the maintenance of immune homeostasis. perturbation of these communities is an emerging characteristic of an increasing number of inflammatory diseases. the goal of this article is to review the current literature on both respiratory and gut microbiomes and their established relationship with allergy and asthma. | 2015 | 26002029 |
| discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis. | under conventional conditions, mice deficient in core 1-derived o-glycans (tm-iec c1galt1(-/-)), which have a defective mucus layer, experienced spontaneous inflammation of the colon. analysis of fecal bacterial populations by pyrosequencing of 16s rrna gene showed that disease in conventional tm-iec c1galt1(-/-) was associated with shifts in the microbiota manifested by increases in lactobacillus and clostridium species, and decreases in unclassified ruminococcaceae and lachnospiraceae. under g ... | 2016 | 24662098 |
| immune system stimulation by probiotic microorganisms. | probiotic organisms are claimed to offer several functional properties including stimulation of immune system. this review is presented to provide detailed informations about how probiotics stimulate our immune system. lactobacillus rhamnosus gg, lactobacillus casei shirota, bifidobacterium animalis bb-12, lactobacillus johnsonii la1, bifidobacterium lactis dr10, and saccharomyces cerevisiae boulardii are the most investigated probiotic cultures for their immunomodulation properties. probiotics ... | 2014 | 24499072 |
| administration of lactobacillus johnsonii fi9785 to chickens affects colonisation by campylobacter jejuni and the intestinal microbiota. | 1. campylobacter jejuni is the most common bacterial cause of human foodborne gastroenteritis in the world largely from contaminated poultry meat. new control measures to reduce or eliminate this pathogen from the animal gastrointestinal tract are urgently required, and the use of probiotics as competitive exclusion agents is a promising biocontrol measure to reduce c. jejuni in the food chain. 2. in this study, we assessed the potential of lactobacillus johnsonii fi9785, which has shown efficac ... | 2017 | 28318296 |
| a highly acid-resistant novel strain of lactobacillus johnsonii no. 1088 has antibacterial activity, including that against helicobacter pylori, and inhibits gastrin-mediated acid production in mice. | a novel strain of lactobacillus johnsonii no. 1088 was isolated from the gastric juice of a healthy japanese male volunteer, and characterized for its effectiveness in the stomach environment. lactobacillus johnsonii no. 1088 was found to have the strongest acid resistance among several lactobacilli examined (>10% of cells survived at ph 1.0 after 2 h), and such a high acid resistance property was a specific characteristic of this strain of l. johnsonii. when cultured with various virulent bacte ... | 2015 | 25771812 |
| controlling of growth performance, lipid deposits and fatty acid composition of chicken meat through a probiotic, lactobacillus johnsonii during subclinical clostridium perfringens infection. | meat is considered as a major source of polyunsaturated fatty acid (pufa) which is essential for humans, therefore its lipid level and fatty acid composition have drawn great attention. as no clinical sign can be found in chicks subclinically infected by clostridium perfringens (cp), the meat may be purchased and eaten. the objective of the present study was to determine whether lactobacillus johnsonii (lj) can control the cp-caused impact on growth, lipid levels, fatty acid composition and othe ... | 2017 | 28183305 |
| microbial shifts associated with necrotic enteritis. | an outbreak of necrotic enteritis (ne) is a complex process requiring one or a number of predisposing factors rather than just the presence of pathogenic clostridium perfringens. examples are dietary influences, such as high levels of non-starch polysaccharides and fishmeal, and factors that evoke epithelial cell damage, such as fusarium mycotoxins in feed and eimeria infections. recent studies have shown that different predisposing factors induce similar shifts in the intestinal microbiota comp ... | 2016 | 26950294 |
| screening of lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics. | lactobacilli are natural inhabitants of human and animal mucous membranes, including the avian gastrointestinal tract. recently, increasing attention has been given to their probiotic, health-promoting capacities, among which their antagonistic potential against pathogens plays a key role. a study was conducted to evaluate probiotic properties of lactobacillus strains isolated from feces or cloacae of domestic geese. among the 104 examined isolates, previously identified to the species level by ... | 2014 | 25104766 |
| application of lactobacillus johnsonii expressing phage endolysin for control of clostridium perfringens. | clostridium perfringens is frequently found in food and the environment and produces potent toxins that have a negative impact on both human and animal health and particularly on the poultry industry. lactobacillus johnsonii fi9785, isolated from the chicken gastrointestinal tract, has been demonstrated to exclude cl. perfringens in poultry. we have investigated the interaction of wild-type lact. johnsonii fi9785 or an engineered strain expressing a cell wall-hydrolysing endolysin with cl. perfr ... | 2014 | 24961379 |
| structure and biosynthesis of two exopolysaccharides produced by lactobacillus johnsonii fi9785. | exopolysaccharides were isolated and purified from lactobacillus johnsonii fi9785, which has previously been shown to act as a competitive exclusion agent to control clostridium perfringens in poultry. structural analysis by nmr spectroscopy revealed that l. johnsonii fi9785 can produce two types of exopolysaccharide: eps-1 is a branched dextran with the unusual feature that every backbone residue is substituted with a 2-linked glucose unit, and eps-2 was shown to have a repeating unit with the ... | 2013 | 24019531 |
| expression and delivery of an endolysin to combat clostridium perfringens. | clostridium perfringens is a cause for increasing concern due to its responsibility for severe infections both in humans and animals, especially poultry. to find new control strategies to treat c. perfringens infection, we investigated the activity and delivery of a bacteriophage endolysin. we identified a new endolysin, designated cp25l, which shows similarity to an n-acetylmuramoyl-l-alanine amidase domain and is distinct from other c. perfringens endolysins whose activity has been demonstrate ... | 2014 | 23942878 |
| effects of the probiotic enterococcus faecium ncimb 10415 on selected lactic acid bacteria and enterobacteria in co-culture. | enterococcus faecium ncimb 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. however, the mode of action of this probiotic modification is still unclear. one possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. reference strain ... | 2015 | 25519527 |
| enhanced expression of fucosyl ga1 in the digestive tract of immune-deficient scid, nude and pigr(-/-) mice. | fucosylation of ga1 in murine intestinal epithelia occurs through transcriptional induction of α1,2-fucosyltransferase along with bacterial infection, but the mechanism has not been clearly characterized as to whether it is induced as a result of an immune response to bacteria or of genetic manipulation of the host by bacteria. accordingly, we analysed the expression of fucosyl ga1 (fga1) and fucosyltransferase activity in the digestive tracts of immune-deficient scid, nude and pigr(-/-) mice. i ... | 2013 | 24089533 |
| design of a beneficial product for newborn calves by combining lactobacilli, minerals, and vitamins. | diarrhea is one of the most frequent diseases affecting newborn calves in intensive systems. several strategies were proposed to protect and improve health, such as probiotics. this work was directed to design a product containing freeze-dried bacteria, vitamins, and minerals, as well as to optimize conditions with lyoprotectors, combine strains and add vitamins, minerals, and inulin to the product. the lyoprotectors were milk, milk-whey, and actose, and products were stored for 6 months at 4°c. ... | 2016 | 26675304 |
| indication for co-evolution of lactobacillus johnsonii with its hosts. | the intestinal microbiota, composed of complex bacterial populations, is host-specific and affected by environmental factors as well as host genetics. one important bacterial group is the lactic acid bacteria (lab), which include many health-promoting strains. here, we studied the genetic variation within a potentially probiotic lab species, lactobacillus johnsonii, isolated from various hosts. | 2012 | 22827843 |
| carbohydrate-binding specificities of potential probiotic lactobacillus strains in porcine jejunal (ipec-j2) cells and porcine mucin. | bacterial lectins are carbohydrate-binding adhesins that recognize glycoreceptors in the gut mucus and epithelium of hosts. in this study, the contribution of lectin-like activities to adhesion of lactobacillus mucosae lm1 and lactobacillus johnsonii pf01, which were isolated from swine intestine, were compared to those of the commercial probiotic lactobacillus rhamnosus gg. both lm1 and pf01 strains have been reported to have good adhesion ability to crude intestinal mucus of pigs. to confirm t ... | 2016 | 27350617 |
| optimizing production of two potential probiotic lactobacilli strains isolated from piglet feces as feed additives for weaned piglets. | two probiotic strains, lactobacillus johnsonii x-1d-2 and lactobacillus mucosae x-4w-1, originally isolated from piglet feces, have been demonstrated to possess antimicrobial activities, antibiotic resistances and interleukin-6 induction ability in raw 267.4 macrophages in our previous study. these characteristics make l. johnsonii x-1d-2 and l. mucosae x-4w-1 good candidates for application in feed probiotics. in this study, soybeal meal, molasses and sodium acetate were selected to optimize th ... | 2015 | 26104525 |
| effects of lactobacillus johnsonii and lactobacillus reuteri on gut barrier function and heat shock proteins in intestinal porcine epithelial cells. | heat shock proteins (hsps) are a set of highly conserved proteins that can serve as intestinal gate keepers in gut homeostasis. here, effects of a probiotic, lactobacillus rhamnosus gg (lgg), and two novel porcine isolates, lactobacillus johnsonii strain p47-hy and lactobacillus reuteri strain p43-huv, on cytoprotective hsp expression and gut barrier function, were investigated in a porcine ipec-j2 intestinal epithelial cell line model. the ipec-j2 cells polarized on a permeable filter exhibited ... | 2015 | 25847917 |
| in vitro evaluation of the mucin-adhesion ability and probiotic potential of lactobacillus mucosae lm1. | in this report, we characterized the probiotic potential of lactobacillus mucosae lm1, focusing on its in vitro mucin-adhesion abilities. | 2014 | 24807045 |