| induction of apoptosis in an insect cell line, iplb-ld652y, infected with nucleopolyhedroviruses. | ld652y cells derived from the gypsy moth, lymantria dispar, were infected with seven different nucleopolyhedroviruses (npvs) including those from autographa californica, bombyx mori (bmnpv), hyphantria cunea (hycunpv), spodoptera exigua (semnpv), l. dispar, orgyia pseudotsugata (opmnpv) and spodoptera litura (spltmnpv). the results showed that ld652y cells infected with bmnpv, hycunpv, semnpv, opmnpv and spltmnpv underwent apoptosis, displaying apoptotic bodies, characteristic dna fragmentation ... | 2003 | 12604823 |
| silencing of the baculovirus op-iap3 gene by rna interference reveals that it is required for prevention of apoptosis during orgyia pseudotsugata m nucleopolyhedrovirus infection of ld652y cells. | the op-iap3 gene from the baculovirus orgyia pseudotsugata m nucleopolyhedrovirus (opmnpv) inhibits apoptosis induced by a mutant of autographa californica mnpv (acmnpv) that lacks the antiapoptotic gene p35, as well as apoptosis induced by a wide range of other stimuli in both mammalian and insect cells. however, the role of op-iap3 during opmnpv infection has not been previously examined. to determine the function of the op-iap3 protein during opmnpv infection, we used rna interference (rnai) ... | 2003 | 12663755 |
| the lymantria dispar nucleopolyhedrovirus contains the capsid-associated p24 protein gene. | during the course of investigations on a wild-type strain of lymantria dispar multinucleocapsid nucleopolyhedrovirus (ldmnpv), a region of the viral genome was analyzed and found to contain 697 bp that is lacking in the sequenced strain (5-6) of ldmnpv (kuzio et al., virology 253, 17-34, 1999). the sequenced strain of ldmnpv contains a mutation in the 25 k few polyhedra (fp) gene, and exhibits the phenotype of a fp mutant. the additional sequence was located at approximately 81.4 map units withi ... | 2003 | 12680688 |
| molecular cloning and sequence analysis of the anticarsia gemmatalis multicapsid nuclear polyhedrosis virus gp64 glycoprotein. | the gp64 locus of anticarsia gemmatalis multicapsid nucleopolyhedrovirus isolate santa fe (agmnpv-sf) was characterised molecularly in our laboratory. to this end, we have located and cloned a agmnpv-sf genomic dna fragment containing the gp64 gene and sequenced the complete gp64 locus. nucleotide sequence analysis indicated that the agmnpv gp64 gene consists of a 1500 nucleotide open reading frame (orf), encoding a protein of 499 amino acids. of the seven gp64 homologues identified to date, the ... | 2003 | 12680694 |
| identification and classification of the spodoptera littoralis nucleopolyhedrovirus inhibitor of apoptosis gene. | baculoviruses possess two types of genes that suppressed apoptosis, p35 and inhibitor of apoptosis (iap). in this study we report the isolation and identification of an inhibitor of apoptosis gene sliap in the genome of the spodoptera littoralis nucleopolyhedrovirus (sinpv). the sliap sequence predicted a 15 kda polypeptide with only one bir domain and a ring finger, both motifs characteristic of the iap family of proteins, and a third specific acidic-rich motif. these characteristics, shared wi ... | 2003 | 12803466 |
| stable expression of recombinant human alpha3/4 fucosyltransferase iii in spodoptera frugiperda sf9 cells. | human alpha3/4 fucosyltransferase iii (ft3; ec 2.4.1.65) synthesizes fucosylated glycoconjugates, namely the lewis (le) determinants. ft3 is detected in milk, gastric mucosa, kidney and other organs, but is found in very low amounts in these native tissues. in this work, we describe the expression of a soluble secretory form of ft3 (sft3) in spodoptera frugiperda (sf9) insect cells using a non-lytic vector system. the coding sequence was cloned into the expression vector pib/v5-his-topo which co ... | 2003 | 14636711 |
| the gp64 locus of anticarsia gemmatalis multicapsid nucleopolyhedrovirus contains a 3' repair exonuclease homologue and lacks v-cath and chia genes. | anticarsia gemmatalis multicapsid nucleopolyhedrovirus (agmnpv) is one of the most successful biological insecticides. in this study, we cloned and sequenced a 12.5 kbp bamhi-d restriction endonuclease fragment of the agmnpv isolate 2d genome that includes the gp64 gene. we compared this highly conserved region with that of other baculoviruses. agmnpv contained two genes, p22.2 and v-trex, in common with choristoneura fumiferana mnpv (cfmnpv) that were not present in other baculoviruses. the v-t ... | 2004 | 14718636 |
| the acidic activation domains of the baculovirus transactivators ie1 and ie0 are functional for transcriptional activation in both insect and mammalian cells. | the acidic activation domains (aads) of the baculovirus transactivators ie1 and ie0 are essential for transcriptional transactivation. to compare the relative transcriptional activation potentials of ie1 and ie0 aads of autographa californica multiple nucleopolyhedrovirus (acmnpv) and orgyia pseudotsugata mnpv (opmnpv), we constructed two ecdysone receptor (ecr)-based inducible expression systems to analyse six baculovirus aads in two insect cell lines (ld652y and sf9) and two mammalian cell lin ... | 2004 | 14993640 |
| laboratory and field evaluation of tebufenozide, diflubenzuron, and bacillus thurengiensis var. kurstaki for suppression of douglas-fir tussock moth (orgyia pseudotsugata (mcdunnough)) in idaho: a case study. | the insect growth regulator tebufenozide (mimic 2lv) was tested to examine its impact on the douglas-fir tussock moth, orgyia pseudotsugata (mcdunnough) (lepidoptera: lymantriidae). laboratory tests gave an estimated concentration of 1.26 ppm of the compound to achieve 50% population mortality (lc50) for second-instar o. pseudotsugata. at the highest concentration tested, tebufenozide resulted in significant larval mortality within 7 d with an estimated time to 50% population mortality (lt50) of ... | 2003 | 14994806 |
| ubiquitin protein ligase activity of the anti-apoptotic baculovirus protein op-iap3. | the baculovirus inhibitor of apoptosis protein (iap) op-iap3 is required to prevent apoptosis during infection of insect cells by orgyia pseudotsugata m nucleopolyhedrovirus (opmnpv) and inhibits apoptosis when overexpressed in insect and mammalian cells. although previous reports have demonstrated that the ring domain is important for the anti-apoptotic function of op-iap3, the function of this domain in op-iap3 has not been studied. here, the ability of op-iap3 to function as an e3 ubiquitin p ... | 2004 | 15325084 |
| european leucoma salicis npv is closely related to north american orgyia pseudotsugata mnpv. | the satin moth leucoma salicis l. (lepidoptera, lymantriidae) is a frequent defoliator of poplar trees (populus spp.) in europe and asia (china, japan). around 1920 the insect was introduced into the usa and canada. in this paper, a multicapsid nucleopolyhedrovirus isolated from l. salicis larvae in poland (lesanpv) was characterized and appeared to be a variant of orgyia pseudotsugata (op) mnpv. o. pseudotsugata, the douglas fir tussock moth (lepidoptera, lymantriidae), occurs exclusively in no ... | 2005 | 15766926 |
| microarray and rt-pcr screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. | here, we report for the first time the successful use of cycloheximide (chx) as an inhibitor to block de novo viral protein synthesis during wssv (white spot syndrome virus) infection. sixty candidate ie (immediate-early) genes were identified using a global analysis microarray technique. rt-pcr showed that the genes corresponding to orf126, orf242 and orf418 in the taiwan isolate were consistently chx-insensitive, and these genes were designated ie1, ie2 and ie3, respectively. the sequences for ... | 2005 | 15780883 |
| tight transcriptional regulation of foreign genes in insect cells using an ecdysone receptor-based inducible system. | the use of insect cells has been highly successful for the expression of foreign proteins from baculoviruses or plasmid vectors. here, we describe a tight transcriptional regulation of foreign genes in insect cells using an ecdysone receptor-based inducible system. the system includes the def domains of the spruce budworm (choristoneura fumiferana) ecr (cfecr) fused to the saccharomyces cerevisiae gal4 dna-binding domain and the ef domains of mammalian mus musculus retinoid x receptor (mmrxr) fu ... | 2005 | 15936954 |
| genotypic variation and presence of rare genotypes among douglas-fir tussock moth multicapsid nucleopolyhedrovirus (opmnpv) isolates in british columbia. | the douglas-fir tussock moth (orgyia pseudotsugata) multicapsid nucleopolyhedrovirus (opmnpv) is periodically applied to suppress douglas-fir tussock moth populations in british columbia and in the western united states. the strain of opmnpv in the product currently used for suppression is not genetically distinct from naturally occurring opmnpv. to separate the mortality caused by the applied virus from that caused by the naturally occurring virus, a rare and genetically distinct strain of opmn ... | 2005 | 15955336 |
| genome mapping and gene analysis of antheraea pernyi nucleopolyhedrovirus for improvement of baculovirus expression vector system. | we have constructed a genome dna map of the antheraea pernyi nucleopolyhedrovirus (anpenpv) and used it to identify target genes for deletion in order to improve the newly developed baculovirus expression vector system. initially, 50 independent psti fragments of viral dna were obtained by shotgun cloning, and both termini of each cloned fragment were sequenced. then, the sequence data were used for homology search against both nucleotide and amino acid sequences of other npvs in databases. this ... | 2002 | 16233185 |
| effects of long-term storage on potency of tm biocontrol-1, the registered viral insecticide of orgyia pseudotsugata (lepidoptera: lymantriidae). | the article presents the results of bioassaying 39 samples of tm biocontrol-1, a viral insecticide, from 10 different lots and various sizes of vacuum-sealed packages that were stored at -10 degrees c for 5-15 yr. this is the first study to present potency data for a registered virus product stored for this length of time. laboratory bioassays in insects from the same colony from which the tm biocontrol-1 was produced showed that the stored viral insecticide is still effective, although it lost ... | 2006 | 16573318 |
| comparison of the structure of c- and n-polyhedrins from two occluded viruses pathogenic for orgyia pseudotsugata. | c-and n-polyhedrins from a cytoplasmic polyhedrosis virus (a double-stranded rna virus) and a nuclear polyhedrosis virus (a dna virus), respectively, of orgyia pseudotsugata were compared. although both polyhedrins appear to stabilize their respective virions and have similar molecular weights, they differed in amino acid composition, tryptic peptide elution profiles from a cation-exchange resin, and n-terminal amino acid sequence and showed no antigenic relatedness. this suggests that these two ... | 1980 | 16789195 |
| gene organization and complete sequence of the hyphantria cunea nucleopolyhedrovirus genome. | the whole-genome sequence of the hyphantria cunea nucleopolyhedrovirus (hycunpv) was analysed. the entire nucleotide sequence of the hycunpv genome was 132 959 bp long, with a g+c content of 45.1 mol%. a total of 148 open reading frames (orfs) consisting of more than 50 aa were encoded by the genome. hycunpv shares more than 122 orfs with other lepidopteran group i npvs, including autographa californica mnpv, bombyx mori npv, choristoneura fumiferana mnpv (cfmnpv), choristoneura fumiferana defec ... | 2006 | 16894193 |
| genome of the most widely used viral biopesticide: anticarsia gemmatalis multiple nucleopolyhedrovirus. | the genome of anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2d (agmnpv-2d), which is the most extensively used virus pesticide in the world, was completely sequenced and shown to have 132 239 bp (g+c content 44.5 mol%) and to be capable of encoding 152 non-overlapping open reading frames (orfs). three orfs were unique to agmnpv-2d, one of which (ag31) had similarity to eukaryotic poly(adp-ribose) polymerases. the lack of chia and v-cath may explain some of the success and growth of ... | 2006 | 17030857 |
| development and evaluation of methods to detect nucleopolyhedroviruses in larvae of the douglas-fir tussock moth, orgyia pseudotsugata (mcdunnough). | various molecular methods are used to detect pathogenic microorganisms and viruses within their hosts, but these methods are rarely validated by direct comparison. southern hybridization, enzyme-linked immunosorbent assay (elisa), and a novel dna extraction/pcr assay were used to detect orgyia pseudotsugata multiple nucleopolyhedrovirus (opmnpv) in douglas-fir tussock moth larvae. pcr was more sensitive than southern hybridization and elisa at detecting semipurified virus. elisa, however, was th ... | 2007 | 17189436 |
| outbreak of lepidopterism at a boy scout camp. | lepidopterism refers to moth- or butterfly-associated illness, including contact dermatitis, urticaria, and occasional systemic reactions. lepidopterism outbreaks are rare. | 2007 | 17368636 |
| development of a dipstick immunoassay to detect nucleopolyhedroviruses in douglas-fir tussock moth larvae. | in this paper, we describe the development of a novel field detection system for the identification of orgyia pseudotsugata nucleopolyhedrovirus (opnpv) and opnpv infections in douglas-fir tussock moth (o. pseudotsugata) (dftm) larvae, utilizing antibodies in a dipstick immunoassay. the dipstick method is sensitive to a minimum of 10ng of extracted virus protein, or approximately 1070 virus occlusion bodies, and is sufficiently sensitive to detect opnpv infections in dftm prior to mortality. add ... | 2007 | 17706298 |
| functional analysis of the transmembrane (tm) domain of the autographa californica multicapsid nucleopolyhedrovirus gp64 protein: substitution of heterologous tm domains. | gp64, the major envelope glycoprotein of the autographa californica multicapsid nucleopolyhedrovirus (acmnpv) budded virion, is important for host cell receptor binding and mediates low-ph-triggered membrane fusion during entry by endocytosis. in the current study, we examined the functional role of the acmnpv gp64 transmembrane (tm) domain by replacing the 23-amino-acid gp64 tm domain with corresponding tm domain sequences from a range of viral and cellular type i membrane proteins, including o ... | 2008 | 18216100 |
| open reading frame 193r of chilo iridescent virus encodes a functional inhibitor of apoptosis (iap). | programmed cell death or apoptosis is a major defense mechanism in insects in response to viral infections. the genome of chilo iridescent virus (civ) has three orfs with homology to baculovirus inhibitor of apoptosis (iap) genes. the proteins encoded by the 157l, 193r, and 332l orfs contain 152, 208 and 234 amino acids, respectively. while all three proteins contain c-terminal ring domains, only the protein encoded by orf 193r contains a baculoviral iap repeat (bir) domain, indicative of a puta ... | 2008 | 18420249 |
| identification, cloning, and r-loop mapping of the polyhedrin gene from the multicapsid nuclear polyhedrosis virus of orgyia pseudotsugata. | polyadenylated rna was isolated from orgyia pseudotsugata larvae 8-10 days postinfection with the multicapsid nuclear polyhedrosis virus. this rna was centrifuged through a sucrose gradient and fractions enriched for polyhedrin mrna were identified by in vitro translation. complementary dna made to this rna hybridized predominantly to a 5-kb fragment of xhoi-digested viral dna. this fragment was cloned into the plasmid pacyc177 and mapped with restriction endonucleases. a sali subclone with a 2. ... | 1982 | 18638748 |
| size determination of orgyia pseudotsugata cytoplasmic polyhedrosis virus genomic rna. | molecular-weight estimates were determined for the orgyia pseudotsugata cytoplasmic polyhedrosis virus (cpv) genome. the molecular-weight estimates were obtained from measurements of glyoxal denatured rna molecules following electrophoresis in 1% agarose gels and electron micrographic contour lengths. these estimates were compared to the minimal coding capacity derived from the size of translation products of selected genomic segments. the results obtained from these different experimental appro ... | 1982 | 18638847 |
| serological analysis of 17 baculoviruses from subgroups a and b using protein blot immunoassay. | we used the protein blot radioimmunoassay technique (smith and summers, 1981) to evaluate the immunoreactivity of antisera made against sds-disrupted baculovirus structural proteins. eight antisera (six for npvs and two for gvs) were tested against eleven nuclear polyhedrosis (npv) and six granulosis viruses (gv). homologous reactions revealed that sds-disrupted baculovirus antigens elicited antibodies which reacted with several of the virus proteins. heterologous reactions of each viral antiser ... | 1983 | 18638912 |
| comparison of sequence diversity in several cytoplasmic polyhedrosis viruses. | sequence homology among several different cytoplasmic polyhedrosis virus (cpv) types and the human reovirus (type 1) was examined by northern blot analysis and s, nuclease analysis, using random-primed cdna probes synthesized from total genomic rna. the results show no homology among the cpv type 1, bombyx mori cpv, type 5, orgyia pseudotsugata cpv, type 8, manduca sexta cpv and the human reovirus (type 1). however, there was significant homology among three type 5 cpvs, o. pseudotsugata cpv, eu ... | 1983 | 18639153 |
| hycu-hr6, a large homologous region of the hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems. | we previously identified a large homologous region (hr), hycu-hr6, in the genome of the hyphantria cunea nucleopolyhedrovirus (hycunpv) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. in this study, we examined the enhancement activity of hycu-hr6 against two promoters from constitutive baculovirus immediate early genes, the hycunpv ie1 (hycu-ie1) and the orgyia pseudotsugata multicapsid (m) npv ie2 (op-ie2), and against a promoter from the inducib ... | 2009 | 19809870 |
| identification of a hyphantria cunea nucleopolyhedrovirus (npv) gene that is involved in global protein synthesis shutdown and restricted bombyx mori npv multiplication in a b. mori cell line. | we previously demonstrated that bombyx mori nucleopolyhedrovirus (bmnpv) multiplication is restricted in permissive bmn-4 cells upon coinfection with hyphantria cunea npv (hycunpv). here, we show that hycunpv-encoded hycu-ep32 gene is responsible for the restricted bmnpv multiplication in hycunpv-coinfected bmn-4 cells. the only homologue for hycu-ep32 is in orgyia pseudotsugata npv. hycu-ep32 could encode a polypeptide of 312 amino acids, and it contains no characteristic domains or motifs to s ... | 2010 | 20034650 |
| host insect inhibitor-of-apoptosis sfiap functionally replaces baculovirus iap but is differentially regulated by its n-terminal leader. | the inhibitor-of-apoptosis (iap) proteins encoded by baculoviruses bear a striking resemblance to the cellular iap homologs of their invertebrate hosts. by virtue of the acquired selective advantage of blocking virus-induced apoptosis, baculoviruses may have captured cellular iap genes that subsequently evolved for virus-specific objectives. to compare viral and host iaps, we defined antiapoptotic properties of sfiap, the principal cellular iap of the lepidopteran host spodoptera frugiperda. we ... | 2010 | 20739517 |
| baculovirus iap1 induces caspase-dependent apoptosis in insect cells. | baculoviruses encode inhibitors of apoptosis (iaps), which are classified into five groups, iap1, iap2, iap3, iap4 and iap5, based on their sequence homology. most of the baculovirus iaps with anti-apoptotic function belong to the iap3 group, with certain exceptions. the functional roles of iaps from other groups in the virus infection have not been well-established. we have previously shown that <em>hyphantria cunea</em> multicapsid npv (hycumnpv) encodes three <em>iap</em> genes, <em>hycu-iap1 ... | 2011 | 21795471 |
| genotypic variation among douglas-fir tussock moth nucleopolyhedrovirus (opnpv) isolates in the western united states. | periodic outbreaks of the douglas-fir tussock moth (orgyia pseudotsugata) in forests of western north america generally end with a sudden collapse due primarily to an epizootic caused by a nucleopolyhedrovirus (npv) that occurs naturally within o. pseudotsugata populations. we genotypically characterized npv populations from washington state, oregon, idaho, new mexico and california for the first time. of 159 infected tussock moth samples, restriction fragment length polymorphism (rflp) analysis ... | 2011 | 21684287 |
| quantification of two viruses in technical preparations of orgyia pseudotsugata baculovirus by means of buoyant density centrifugation of viral deoxyribonucleic acid. | a reliable method was developed for the quantitative determination of two nuclear polyhcdrosis viruses present in commercially prepared viral insecticides used against orgyia pseudotsugata. deoxyribonucleic acids, from nuclear polyhedrosis bundle virus and nuclear polyhedrosis single-rod virus, were separated on cscl gradients according to their respective buoyant densities, 1.715 and 1.704 g/ml. the proportions of the two viruses were quantified by measuring the relative absorbance at 254 nm of ... | 1983 | 348107 |
| the genome of dasychira pudibunda nucleopolyhedrovirus (dapunpv) reveals novel genetic connection between baculoviruses infecting moths of the lymantriidae family. | dapunpv (dasychira pudibunda nucleopolyhedrovirus), presented in this report, belongs to alphabaculovirus group ib. its full, newly sequenced genome shows close relationship to baculovirus opmnpv isolated from douglas-fir tussock moth orgyia pseudotsugata. baculovirus dapunpv is a natural limiter of pale tussock moth dasychira pudibunda l. (syn. calliteara pudibunda l.)(lepidoptera, lymantriidae), which can occur in a form of an outbreak on many species of deciduous trees and may cause significa ... | 2015 | 26449402 |
| gene organization and sequencing of the choristoneura fumiferana defective nucleopolyhedrovirus genome. | two distinct nucleopolyhedrovirus species of the eastern spruce budworm, choristoneura fumiferana, exist in a symbiont-like relationship. c. fumiferana defective nucleopolyhedrovirus (cfdefnpv) only infects c. fumiferana larvae per os in the presence of c. fumiferana nucleopolyhedrovirus ireland strain (cfmnpv), but is infective when injected into the haemolymph. cfdefnpv synergizes cfmnpv in per os infections and cfmnpv is always the predominant progeny. this study was undertaken to report the ... | 2005 | 15784888 |
| effect of bbx-b8 overexpression on development, body weight, silk protein synthesis and egg diapause of bombyx mori. | bombyxin (bbx) is an insulin-like peptide exists in the silkworm bombyx mori. our previous studies on the effects of inhibiting bbx-b8 expression found that bbx-b8 is important for the development of organ, reproduction and trehalose metabolism in the silkworms. in this paper, we investigated the expression profile of the bbx-b8 gene and effect of bbx-b8 overexpression on the development, body weight, silk protein synthesis and egg diapause of b. mori to further understand bbx-b8 functions. bbx- ... | 2016 | 26951193 |
| baculovirus lymantria dispar multiple nucleopolyhedrovirus iap2 and iap3 do not suppress apoptosis, but trigger apoptosis of insect cells in a transient expression assay. | ld652y cells derived from the gypsy moth, lymantria dispar, are permissive for productive infection with l. dispar multiple nucleopolyhedrovirus (ldmnpv), but undergo apoptosis upon infection with various other npvs, including those isolated from bombyx mori, hyphantria cunea, spodoptera exigua, orgyia pseudotsugata, and spodoptera litura. in this study, we examined whether ldmnpv-encoded inhibitor of apoptosis 2 (ld-iap2) and 3 (ld-iap3) are involved in apoptosis suppression in ldmnpv-infected ... | 2012 | 22798056 |
| distinct gene arrangement in the buzura suppressaria single-nucleocapsid nucleopolyhedrovirus genome. | the genome organization of the buzura suppressaria single-nucleocapsid nucleopolyhedrovirus (busunpv) was largely elucidated and compared to those of other baculoviruses. a detailed physical map was constructed for the restriction enzymes bamhi, bgli, bglii, ecori, hindiii, kpni, psti, xbai and xhoi. the 120.9 kbp viral genome was cloned as restriction fragments into a plasmid library from which about 43.5 kbp of dispersed sequence information was generated. fifty-two putative open reading frame ... | 1998 | 9820162 |
| n-terminal polyhedrin sequences and occluded baculovirus evolution. | a phylogenetic tree for occluded baculoviruses was constructed based on the n-terminal amino acid sequence of occlusion body proteins from six baculoviruses including three lepidopteran nuclear polyhedrosis viruses (npvs), [two unicapsid (bombyx mori and orgyia pseudotsugata) and one multicapsid (orgyia pseudotsugata)]; one granulosis virus (pieris brassicae); and npvs from a hymenopteran (neodiprion sertifer) and a dipteran (tipula paludosa). amino acid sequence data for the b. mori npv were fr ... | 1981 | 7026796 |
| tryptic peptide analysis and nh2-terminal amino acid sequences of polyhedrins of two baculoviruses from orgyia pseudotsugata. | comparative analysis of the tryptic peptides and terminal amino acid sequence was made on polyhedrins from two genetically different baculoviruses that are naturally pathogenic for the same insect host. comparison of the tryptic peptides of the nucleopolyhedrosis bundle virus and nucleopolyhedrosis single-rod virus of orgyia pseudotsugata by means of cation-exchange resins indicated that the proteins have a closely related amino acid sequence. the nh(2)-terminal amino acid sequence of polyhedrin ... | 1979 | 291914 |
| analysis of the choristoneura fumiferana nucleopolyhedrovirus genome. | the double-stranded dna genome of choristoneura fumiferana nucleopolyhedrovirus (cfmnpv) was sequenced and analysed in the context of other group i nucleopolyhedroviruses (npvs). the genome consists of 129,593 bp with a g + c content of 50.1 mol%. a total of 146 open reading frames (orfs) of greater than 150 bp, and with no or minimal overlap were identified. in addition, five homologous regions were identified containing 7-10 repeats of a 36 bp imperfect palindromic core. comparison with other ... | 2005 | 15784887 |
| molecular analysis of the p48 gene of choristoneura fumiferana multicapsid nucleopolyhedroviruses cfmnpv and cfdefnpv. | attempts were made to linearize the dna of choristoneura fumiferana (cf) multicapsid nucleopolyhedrovirus (mnpv), in order to improve the efficiency of generation of recombinant viruses after transfection. a unique site for the restriction enzyme sse83871 was found in orf p48. the requirement for this orf during virus replication was investigated by molecular analyses including sequencing, transcriptional analysis and inactivation by insertion of marker genes. sequence analysis showed that orf p ... | 1999 | 10423153 |
| the baculovirus anti-apoptotic protein op-iap does not inhibit drosophila caspases or apoptosis in drosophila s2 cells and instead sensitizes s2 cells to virus-induced apoptosis. | the op-iap protein from the baculovirus orgyia pseudotsugata m nucleopolyhedrovirus (opmnpv) is highly effective at inhibiting apoptosis triggered by a variety of different stimuli in lepidopteran cells as well as in several different mammalian cell types, suggesting that it functions at a highly conserved step in the apoptotic pathway. however, the mechanism by which op-iap inhibits apoptosis is unclear. since some iap proteins can bind and inhibit caspases, we tested whether op-iap could inhib ... | 2005 | 15823606 |