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molecular detection of bartonella species in fleas collected from dogs and cats from costa rica.the bacterial genus bartonella includes several species with zoonotic potential, some of which are common in domestic dogs and cats, as well as in their fleas. because there is no previous information about the presence of bartonella species in fleas from central america, this study aimed at evaluating the presence of bartonella spp. in fleas collected from dogs and cats in costa rica. a total 72 pools of ctenocephalides felis and 21 pools of pulex simulans were screened by conventional pcr to d ...201526393956
analysis of seroreactivity against cell culture-derived bartonella spp. antigens in dogs.little is known about the specificity of bartonella spp. immunofluorescent antibody (ifa) assays in dogs. bacteremia in sick dogs most often has been associated with bartonella henselae (bh), bartonella vinsonii subspecies berkhoffii (bvb), and bartonella koehlerae (bk). clarification of the diagnostic utility of ifa serology when testing against these organisms is needed.201724341682
experimental infection of dogs with bartonella henselae and bartonella vinsonii subsp. berkhoffii.the lack of a suitable infection model remains an important obstacle for the pathophysiological understanding of bartonella spp. the following pilot study was designed to determine whether cell culture-grown bartonella henselae sa2 and bartonella vinsonii subsp. berkhoffii genotype iii would cause persistent bacteremia in dogs. pre-inoculation screening established that two laboratory-raised golden retrievers were naturally-infected with bartonella koehlerae. despite prior infection, one dog eac ...201324120155
identification of bartonella species isolated from rodents from yucatan, mexico, and isolation of bartonella vinsonii subsp. yucatanensis subsp. nov.bartonella species are highly endemic among wild rodents in many parts of the world. blood and/or blood clot cultures from 38 rodents, including 27 yucatan deer mouse (peromyscus yucatanicus), 7 gaumer's spiny pocket mouse (heteromys gaumeri), 2 black rats (rattus rattus) and 2 big-eared climbing rats (ototylomys phyllotis) captured near merida, yucatan, mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as bartonella spp. based on colony morphology. d ...201627626126
hepatic granulomatous lesions caused by systemic bartonella vinsonii subsp. arupensis infection in a child. 201526780031
molecular survey of bartonella species and yersinia pestis in rodent fleas (siphonaptera) from chihuahua, mexico.rodent fleas from northwestern chihuahua, mexico, were analyzed for the presence of bartonella and yersinia pestis. in total, 760 fleas belonging to 10 species were tested with multiplex polymerase chain reaction analysis targeting the glta (338-bp) and pla genes (478-bp) of bartonella and y. pestis, respectively. although none was positive for y. pestis, 307 fleas were infected with bartonella spp., resulting in an overall prevalence of 40.4%. a logistic regression analysis indicated that the p ...201626576933
zoonotic bartonella species in wild rodents in the state of mato grosso do sul, brazil.several rodent-associated bartonella species cause disease in humans but little is known about their epidemiology in brazil. the presence of bartonella spp. in wild rodents captured in two municipalities of the mato grosso do sul state was assessed by polymerase chain reaction (pcr). fragments of heart tissue from 42 wild rodents were tested using primers targeting the bartonella 16s-23s intergenic transcribed spacer (its) region and citrate synthase glta gene. the wild rodents were identified b ...201626344604
increased erythrocytic osmotic fragility in anemic domestic shorthair and purebred cats.increased erythrocytic osmotic fragility and splenomegaly have been reported in anemic abyssinian and somali cats. here we report on this condition in anemic domestic shorthair cats and two other breeds, and describe common features of the clinicopathological profiles, management and outcomes.201626023192
prevalence and genetic diversity of bartonella strains in rodents from northwestern mexico.bartonella infections were investigated in wild rodents from northwestern chihuahua, mexico. a total of 489 rodents belonging to 14 species were surveyed in four areas. bartonella bacteria were cultured from 50.1% of rodent samples (245/489). infection rates ranged from 0% to 83.3% per rodent species, with no significant difference between sites except for cynomys ludovicianus. phylogenetic analyses of the citrate synthase gene (glta) of the bartonella isolates revealed 23 genetic variants (15 n ...201425514119
[real-time pcr-based detection of bartonella vinsonii sub sp. berkhoffii by taqman minor groove binder probe].bartonella vinsonii subsp. berkhoffii is a fastidious haemotropic gram-negative bacterium that has been identified as an emerging causative agent for zoonotic diseases of human and dogs. this study aimed to develop a taqman-mgb probe based, highly sensitive and species-specific fluorescence quantitative pcr assay for rapid detection of b. vinsonii subsp. berkhoffii.201324377250
spontaneous onset of complex regional pain syndrome type i in a woman infected with bartonella koehlerae.after a short-term fever, complex regional pain syndrome, characterized by hyperalgesia, intermittent swelling, erythema and cyanosis of both feet, was diagnosed in a female veterinarian. the woman was infected with bartonella koehlerae and she was also bartonella vinsonii subsp. berkhoffii seroreactive. having failed other treatments, symptoms resolved following initiation of antibiotics.201424323298
bartonella vinsonii subsp. berkhoffii in free-ranging white-tailed deer (odocoileus virginianus).bartonella vinsonii subsp. berkhoffii has not been detected previously in white-tailed deer (odocoileus virginianus). we tested whole blood from 60 white-tailed deer for bartonella spp. dna; three (5%) were positive for bartonella vinsonii subsp. berkhoffii. this is the first detection of bartonella vinsonii subsp. berkhoffii in white-tailed deer.201323568932
prevalence and potential risk factors for bartonella infection in tunisian stray dogs.bartonellae are blood-borne and vector-transmitted pathogens, some are zoonotic, which have been reported in several mediterranean countries. transmission from dogs to humans is suspected, but has not been clearly demonstrated. our objectives were to determine the seroprevalence of bartonella henselae, bartonella vinsonii subsp. berkhoffii, bartonella clarridgeiae, and bartonella bovis (as a proxy for candidatus bartonella merieuxii) in stray dogs from tunisia, identify the bartonella species in ...201728346866
bartonella vinsonii subsp. arupensis in humans, thailand.we identified bartonella vinsonii subsp. arupensis in pre-enriched blood of 4 patients from thailand. nucleotide sequences for transfer-messenger rna gene, citrate synthase gene, and the 16s-23s rrna internal transcribed spacer were identical or closely related to those for the strain that has been considered pathogenic since initially isolated from a human in wyoming, usa.201222607728
bartonella vinsonii endocarditis in an adolescent with congenital heart disease.we report the second human case of bartonella vinsonii subsp. berkhoffii endocarditis in a 19-year-old male with congenital heart disease. diagnosis was established by bartonella antibodies detected by immunofluorescence, and polymerase chain reaction amplification and sequencing from blood.201222301474
seroepidemiology of bartonella infection in gray foxes from texas.gray foxes (urocyon cinereoargenteus) were shown to be naturally infected with bartonella rochalimae, a bartonella species similar to bartonella clarridgeiae (b.c.), and bartonella vinsonii subspecies berkhoffii (b.v.berkhoffii) in northern california. a serological survey was performed to investigate the presence of bartonella infection in 132 gray foxes from west/central texas. using an immunofluorescence antibody test directed against b.v.berkhoffii and b.c., the antibody prevalence was 50% ( ...201222217178
zoonotic pathogens in atlantic forest wild rodents in brazil: bartonella and coxiella infections.zoonotic pathogens comprise a significant and increasing fraction of all emerging and re-emerging infectious diseases that plague humans. identifying host species is one of the keys to controlling emerging infectious diseases. from march 2007 until april 2012, we collected a total of 131 wild rodents in eight municipalities of rio de janeiro, brazil. we investigated these rodents for infection with coxiella burnetii, bartonella spp. and rickettsia spp. in total, 22.1% (29/131) of the rodents wer ...201728077317
prevalence of bartonella spp. in canine cutaneous histiocytoma.canine cutaneous histiocytoma (cch) is a common, benign neoplastic proliferation of histiocytes of langerhans cell origin that often ulcerate, become secondarily infected and regress spontaneously. bartonella is a fastidious genus of facultative intracellular pathogens that can be transmitted through arthropod bites and epidermal animal scratches and has been identified previously in the cytoplasm of histiocytes within granulomatous lesions and in skin biopsy samples of inflammatory pustules and ...201525980841
bartonella vinsonii subsp. berkhoffii and bartonella henselae as potential causes of proliferative vascular diseases in animals.bartonella species are highly fastidious, vector borne, zoonotic bacteria that cause persistent intraerythrocytic bacteremia and endotheliotropic infection in reservoir and incidental hosts. based upon prior in vitro research, three bartonella sp., b. bacilliformis, b. henselae, and b. quintana can induce proliferation of endothelial cells, and each species has been associated with in vivo formation of vasoproliferative tumors in human patients. in this study, we report the molecular detection o ...201222450733
death of military working dogs due to bartonella vinsonii subspecies berkhoffii genotype iii endocarditis and myocarditis.as a result of extensive field-related activities, military working dogs (mwds) have an increased occupational risk for acquiring vector-borne infectious diseases.201728290975
prevalence and phylogenetic analysis of bartonella species of wild carnivores and their fleas in northwestern mexico.the host-parasite-vector relationship of bartonella spp. system in wild carnivores and their fleas from northwestern mexico was investigated. sixty-six carnivores belonging to eight species were sampled, and 285 fleas belonging to three species were collected during spring (april-may) and fall (october-november) seasons. we detected bartonella species in 7 carnivores (10.6%) and 27 fleas (9.5%) through either blood culture or pcr. of the 27 bartonella-positive fleas, twenty-two were pulex simula ...201728197898
bartonella rochalimae and b. vinsonii subsp. berkhoffii in wild carnivores from colorado, usa.spleen samples from 292 wild carnivores from colorado, us were screened for bartonella infection. bartonella dna was detected in coyotes ( canis latrans ) (28%), striped skunks ( mephitis mephitis ) (23%), red foxes ( vulpes vulpes ) (27%), and raccoons ( procyon lotor ) (8%) but not in black bears ( ursus americanus ), gray foxes ( urocyon cinereoargenteus ), and mountain lions ( puma concolor ). two bartonella species, b. vinsonii subsp. berkhoffii and b. rochalimae, were identified. all 10 in ...201627529290
detection of bartonella spp. in wild carnivores, hyraxes, hedgehog and rodents from israel.bartonella infection was explored in wild animals from israel. golden jackals (canis aureus), red foxes (vulpes vulpes), rock hyraxes (procavia capensis), southern white-breasted hedgehogs (erinaceus concolor), social voles (microtus socialis), tristram's jirds (meriones tristrami), cairo spiny mice (acomys cahirinus), house mice (mus musculus) and indian crested porcupines (hystrix indica) were sampled and screened by molecular and isolation methods. bartonella-dna was detected in 46 animals: 9 ...201627210612
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